14 results about "Potato virus Y" patented technology

The present application discloses a short peptide PRPl, and a preparation method and a use thereof, belonging to the technical field of genetic engineering. Through research, the present application identifies a short peptide derived from an unknown protein that regulates tobacco resistance to potato virus Y (PVY) disease, named PVY resistance peptide (PRP 1). Its amino acid sequence is ATKHIVGSIVAS, and the short peptide PRPl is prepared by chemical synthesis. The use of the short peptide injection during the seedling stage of tobacco may effectively reduce the amount of PVY virus and improve the resistance of tobacco to PVY disease.

This application discloses a short peptide PRP1, its preparation method, and its application. It belongs to the field of genetic engineering technology. This application identifies a short peptide derived from an unknown protein that can regulate tobacco resistance to Potato Virus Y (PVY), named PVY resistance peptide (PRP1). Its amino acid sequence is ATKHIVGSIVAS. The short peptide PRP1 was prepared through chemical synthesis. Injecting this short peptide into tobacco seedlings can effectively reduce PVY virus levels and improve tobacco resistance to PVY.

This invention provides a molecular marker for detecting the PVR4 gene, a resistance gene against Potato Virus Y, and its application, belonging to the field of genetic engineering technology. The molecular marker is the TC base at 4714-4715 bp of the CM334 pepper variety (NCBI accession number KT359375.1). This invention also provides primers and a kit based on the molecular marker. PCR amplification and electrophoresis detection enable rapid and accurate screening of pepper varieties carrying the PVR4 gene against Potato Virus Y. The molecular marker described in this invention plays an important role in detecting the PVR4 gene against Potato Virus Y in peppers or in breeding disease-resistant pepper varieties.

The present application provides a virus detoxification method of Chinese yam. The present application can effectively prevent Japanese yam mosaic virus (JYMV), potato virus Y (PVY), potato virus X (PVX) and potato leaf roll virus (PLRV) by peeling the stem tip of Chinese yam and using heat treatment and ribavirin detoxification. The optimal virus removal method is to peel the stem tip to 0.3-0.5mm, heat treatment at 40℃ for 8h, and ribavirin 50mg / L.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an NtSGRL1-related biological product in tobacco antivirus, and the nucleotide sequence of the NtSGRL1 is shown as SEQ ID NO.1. The invention also relates to an application of the NtSGRL1-related biological product in tobacco antivirus. According to the invention, a CRISPR-Cas9 mediated common tobacco NtSGRL1 knockout strain Ntsgrl1 is created through genetic transformation, and four main tobacco viruses of tomato yellow leaf curl virus, tobacco mosaic virus, potato Y virus and tomato spotted wilt virus are respectively adopted to inoculate the obtained Ntsgrl1 function deletion mutant strain. Disease resistance identification results show that compared with wild and overexpression strains, the Ntsgrl1 mutant strain shows broad-spectrum and significantly enhanced resistance to four tested viruses, which is specifically embodied in that the system symptom development is significantly inhibited, and the virus accumulation in the plant is significantly reduced.

The present invention relates to the use of triazole compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an NbPPO-related biological product in improving the antiviral capacity of plants. According to the invention, through fluorescent quantification, the expression quantity of NbPPO is obviously increased after nicotiana benthamiana is infected by potato Y virus and capsicum vein mottle virus; after NbPPO expression is silenced, the RNA accumulation amount of the virus in nicotiana benthamiana infected by the potato Y virus is obviously increased; when the recombinant agrobacterium containing the NbPPO recombinant plasmid is used for infecting nicotiana benthamiana, nicotiana benthamiana with transient overexpression of NbPPO is obtained, and it is found that nicotiana benthamiana with transient overexpression of NbPPO can effectively inhibit virus infection. Therefore, overexpression of the NbPPO can improve the antiviral ability of the plants, and genetic improvement can be performed on the antiviral infection ability of the plants by using NbPPO-related biological products.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

The present invention relates to the use of triazole compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The present invention relates to the use of benzo[B][1,8]naftiridine-based compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The application belongs to the field of agricultural chemicals, and particularly relates to a compound with anti-potato virus Y virus activity and a preparation method and application thereof. 16 H 23 N5O7, the compound provided by the application has good inhibiting effect on viruses of the potato virus Y, such as potato virus Y, sugarcane mosaic virus and turnip mosaic virus, and can be used for preparation of anti-plant virus pesticides, and has wide application prospect.

This invention discloses a hybridoma cell line secreting monoclonal antibody PVY-2, its monoclonal antibody, and its applications. The hybridoma cell line 4F1B2G11 secretes a monoclonal antibody against potato virus Y. The preservation number of the hybridoma cell line is CCTCC NO: C2022281. The monoclonal antibody PVY-2 has a specific immunoreactivity with the coat protein of potato virus Y. The nucleotide sequence of the heavy chain variable region of monoclonal antibody PVY-2 is shown in SEQ ID NO.4, and the nucleotide sequence of the light chain variable region is shown in SEQ ID NO.5. The sensitivity of the direct ELISA method for detecting potato virus Y-infected leaves reaches a 1:5120 dilution. The monoclonal antibody PVY-2 can also be used to prepare ELISA kits, colloidal gold kits, or nano-enzyme kits, enabling rapid, sensitive, economical, and accurate detection of potato virus Y disease.

The application discloses an application of Terminalia chebula gallae in resisting potato virus Y, and belongs to the technical field of biotechnology. The application takes the potato virus Y VPg protein as a target, and successfully obtains the candidate compound Terminalia chebula gallae through virtual screening, and verifies high affinity binding of the candidate compound with the potato virus Y VPg and an antiviral effect from three levels of a molecular level, an in-vitro interaction and a living body level. The results show that the Terminalia chebula gallae can effectively inhibit the spread and infection of the potato virus Y on plants, and relieve the plant disease process. The application provides a theoretical basis and an experimental basis for antiviral drug design based on the VPg target, and lays a foundation for subsequent structure optimization of the Terminalia chebula gallae, development of green pesticides and in-depth analysis of an action mechanism.