27 results about "Potato virus Y" patented technology

The present application discloses a short peptide PRPl, and a preparation method and a use thereof, belonging to the technical field of genetic engineering. Through research, the present application identifies a short peptide derived from an unknown protein that regulates tobacco resistance to potato virus Y (PVY) disease, named PVY resistance peptide (PRP 1). Its amino acid sequence is ATKHIVGSIVAS, and the short peptide PRPl is prepared by chemical synthesis. The use of the short peptide injection during the seedling stage of tobacco may effectively reduce the amount of PVY virus and improve the resistance of tobacco to PVY disease.

This application discloses a short peptide PRP1, its preparation method, and its application. It belongs to the field of genetic engineering technology. This application identifies a short peptide derived from an unknown protein that can regulate tobacco resistance to Potato Virus Y (PVY), named PVY resistance peptide (PRP1). Its amino acid sequence is ATKHIVGSIVAS. The short peptide PRP1 was prepared through chemical synthesis. Injecting this short peptide into tobacco seedlings can effectively reduce PVY virus levels and improve tobacco resistance to PVY.

This invention provides a molecular marker for detecting the PVR4 gene, a resistance gene against Potato Virus Y, and its application, belonging to the field of genetic engineering technology. The molecular marker is the TC base at 4714-4715 bp of the CM334 pepper variety (NCBI accession number KT359375.1). This invention also provides primers and a kit based on the molecular marker. PCR amplification and electrophoresis detection enable rapid and accurate screening of pepper varieties carrying the PVR4 gene against Potato Virus Y. The molecular marker described in this invention plays an important role in detecting the PVR4 gene against Potato Virus Y in peppers or in breeding disease-resistant pepper varieties.

The present application provides a virus detoxification method of Chinese yam. The present application can effectively prevent Japanese yam mosaic virus (JYMV), potato virus Y (PVY), potato virus X (PVX) and potato leaf roll virus (PLRV) by peeling the stem tip of Chinese yam and using heat treatment and ribavirin detoxification. The optimal virus removal method is to peel the stem tip to 0.3-0.5mm, heat treatment at 40℃ for 8h, and ribavirin 50mg / L.

The invention discloses an immune receptor-single-chain antibody fusion protein and application of the immune receptor-single-chain antibody fusion protein in prevention and treatment of potato virus Y (pota virus Y, PVY) and tomato brown fruit ruffle virus (ToBRFV), and belongs to the technical field of biological prevention and treatment of viral diseases. According to the invention, a modified immune receptor Sr35 (Sr3527) and a single-chain antibody scFvM1 or scFv16 are fused to create an immune receptor-single-chain antibody fusion protein, and the immune receptor-single-chain antibody fusion protein can specifically recognize PVY coat protein (CP) or CP of ToBRFV, activate host resistance and generate hypersensitive response (HR); after being expressed in plants, the bacillus subtilis can inhibit infection of PVY or ToBRFV, and has a remarkable prevention and treatment effect on PVY or ToBRFV.

The invention discloses miR159a related to plant antivirus and application of the miR159a in improvement of virus resistance of plants, and belongs to the technical field of genetic engineering. The miR159a related to the plant antivirus is nbe-miR159a, and the nucleotide sequence of the miR159a related to the plant antivirus is shown as SEQ ID NO: 1. The target gene of the nbe-miR159a is a CPR5 gene, an overexpression vector of the nbe-miR159a is constructed, the overexpression vector is converted into a tobacco plant, the expression quantity of the nbe-miR159a in tobacco is increased, and the relative expression quantity of CPR5 can be reduced, so that the resistance of the tobacco to the potato Y virus is improved. Therefore, the nbe-miR159a has an important application prospect in prevention and treatment of plant virus diseases and improvement of virus resistance of plants.

The present invention provides a trivalent attenuated vaccine against PVY, PVX and CMV and its application, and the present invention belongs to the field of plant virology and molecular biology. The trivalent attenuated vaccine contains a mutant plasmid pCB3 of cucumber mosaic virus (CMV) RNA2. F R2‑2bPT‑PVY 8093‑8292 PVX 85‑284 The sequence of the mutant plasmid is shown in SEQ ID NO.3. Fny Plasmid pCB from isolate RNA1 F R1 and CMV Fny Isolate RNA3 aphid-transmitted site mutation plasmid pCB F R3‑△apno is pre-mixed with mutant plasmids for inoculation to prevent potato virus Y, potato virus X and cucumber mosaic virus. At the same time, the vaccine can prevent aphids from transmitting the virus and avoid biosafety risks, providing vaccine materials and effective measures for field control of PVY, PVX and CMV.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an NtSGRL1-related biological product in tobacco antivirus, and the nucleotide sequence of the NtSGRL1 is shown as SEQ ID NO.1. The invention also relates to an application of the NtSGRL1-related biological product in tobacco antivirus. According to the invention, a CRISPR-Cas9 mediated common tobacco NtSGRL1 knockout strain Ntsgrl1 is created through genetic transformation, and four main tobacco viruses of tomato yellow leaf curl virus, tobacco mosaic virus, potato Y virus and tomato spotted wilt virus are respectively adopted to inoculate the obtained Ntsgrl1 function deletion mutant strain. Disease resistance identification results show that compared with wild and overexpression strains, the Ntsgrl1 mutant strain shows broad-spectrum and significantly enhanced resistance to four tested viruses, which is specifically embodied in that the system symptom development is significantly inhibited, and the virus accumulation in the plant is significantly reduced.

The invention discloses an application of a terpenoid natural product containing an isoprene unit as an agricultural bactericide in plant diseases, the terpenoid natural product containing the isoprene unit is composed of two or more isoprene units, and the terpenoid natural product containing the isoprene unit aims at plant viral diseases such as tobacco mosaic virus, cucumber mosaic virus or potato Y virus; aiming at plant fungal diseases such as cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab, potato late blight, blueberry root rot, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum capsici; the compound has a good inhibition effect on plant bacterial diseases such as rice bacterial leaf blight, citrus canker, tobacco bacterial wilt, kiwifruit canker or rice bacterial leaf streak. The invention provides new application of terpenoid natural products, and the terpenoid natural products can be used for preventing and treating plant bacterial diseases, fungal diseases and plant viral diseases in the agricultural field.

The present invention relates to the use of triazole compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The present invention relates to a 2,2-dimethyl-2H-chromene derivative containing a sulfonamide unit, a preparation method thereof and an application. The compound of the present invention has the structure of the following formula (I), which has excellent inhibitory activity against plant viruses such as potato virus Y, and can be used as a drug or medicament for preventing and treating plant viral diseases such as potato virus Y disease. It has a novel structure, a simple preparation process and low production cost.

The present invention discloses a nano-mimetic enzyme test strip containing two pairable PVY monoclonal antibodies and its application. The two pairable PVY monoclonal antibodies are PVY-2 and PVY-5; PVY-2 is secreted by the hybridoma cell line 4F1B2G11 with a preservation number of CCTCC NO: C2022281, and PVY-5 is secreted by the hybridoma cell line 4B4D8C3 with a preservation number of CCTCC NO: C2022282; PVY-5 is used as a labeled antibody and PVY-2 is used as a coating antibody to prepare a PVY nano-mimetic enzyme test strip. The test strip has a detection sensitivity of 10 for leaves infected with potato virus Y. ‑4 (w / v, g / mL). The research and development of this product will fill the gap in the field of nano-mimic enzymes in potato plant virus detection.

The invention discloses a medicine for preventing and treating plant diseases as well as a preparation method and application thereof, and belongs to the technical field of biopesticides. The medicine for preventing and treating the plant diseases, disclosed by the invention, is prepared from hydrogel and nbe-miR159a; the nucleotide sequence of the nbe-miR159a is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The preparation method comprises the following steps: coating nbe-miR159a by using a pH responsive isoGPBG hydrogel; the plant miRNA transmembrane transport obstacle is broken through, efficient delivery and controllable release of the antiviral nucleic acid medicine are achieved, and miRNA can be protected from being degraded. The drug has a good inhibition effect on potato virus Y and ralstonia solanacearum, and has an important application prospect in prevention and treatment of plant bacterial diseases and viral diseases.

The present invention discloses the application of plant 14-3-3h protein and its coding gene in resisting Potato virus Y, belonging to the field of biotechnology. The present invention for the first time studies and proves that plant 14-3-3h protein can interact with 6K1 protein participating in the replication process in Potato virus Y; silencing the 14-3-3h gene in plants will promote the infection of PVY; while overexpressing the 14-3-3h gene will inhibit the infection of PVY. Therefore, plant 14-3-3h protein can be used as a new target for resisting Potato virus Y, providing a new idea for the prevention and control of Potato virus Y disease.

The invention discloses a potato Y virus NIb protein attenuated mutant and application thereof, and belongs to the technical field of plant genetic engineering. The 233 lysine of NIb protein in PVY is mutated into glutamine, a mutant with obviously reduced pathogenicity is obtained, and the accumulation levels of CP and GFP in the inoculated mutant NIbK233Q nicotiana rubensis are lower than those of wild type PVY. Moreover, the mutant has hereditary stability, does not generate reverse mutation after subculture, and has use safety. The mutant (NIbK233Q) provided by the invention can be used as an attenuated vaccine, can prevent plants such as tobacco and the like from being damaged by PVY virulent strains through cross protection, and has important significance in the aspect of PVY prevention and treatment.

The present application discloses a monoclonal antibody for detecting potato virus Y and its application. The heavy chain variable region comprises: the heavy chain CDR1 shown in SEQ ID No. 1, the heavy chain CDR2 shown in SEQ ID No. 2, and the heavy chain CDR3 shown in SEQ ID No. 3; the light chain variable region comprises: the light chain CDR1 shown in SEQ ID No. 4, the light chain CDR2 shown in SEQ ID No. 5, and the light chain CDR3 shown in SEQ ID No. 6. The monoclonal antibody can be directly made into a detection device, such as a colloidal gold test strip, for rapid detection of large quantities of samples. The colloidal gold test strip made with the antibody has a detection limit of up to 300 ng / mL for PVY-CP protein; and has good specificity, with negative detection results for viruses such as PVS, PLRV, and PVM.

The invention belongs to the technical field of plant virus detection, and particularly relates to a multiplex PCR (Polymerase Chain Reaction) detection method for three potato Y viruses on passionflower, which is a multiplex RT-PCR detection method for simultaneously detecting the three potato Y viruses of TeMV, EAPV and PFSMoV. The multiplex PCR detection method comprises the following steps: genome extraction: extracting total RNA of a sample to be detected, and then performing reverse transcription to obtain cDNA; multiple RT-PCR amplification reaction: taking the obtained cDNA as a detection template, and carrying out multiple RT-PCR amplification on the obtained cDNA and specific primers of TeMV, EAPV and PFSMoV viruses under the condition of a reaction system; and result detection: determining the result of the amplification product by means of agarose gel electrophoresis. The invention successfully establishes a multiplex PCR detection method for three potato Y viruses on passionflower, and the detection method can simultaneously detect three potato Y viruses of TeMV, EAPV and PFSMoV.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an NbPPO-related biological product in improving the antiviral capacity of plants. According to the invention, through fluorescent quantification, the expression quantity of NbPPO is obviously increased after nicotiana benthamiana is infected by potato Y virus and capsicum vein mottle virus; after NbPPO expression is silenced, the RNA accumulation amount of the virus in nicotiana benthamiana infected by the potato Y virus is obviously increased; when the recombinant agrobacterium containing the NbPPO recombinant plasmid is used for infecting nicotiana benthamiana, nicotiana benthamiana with transient overexpression of NbPPO is obtained, and it is found that nicotiana benthamiana with transient overexpression of NbPPO can effectively inhibit virus infection. Therefore, overexpression of the NbPPO can improve the antiviral ability of the plants, and genetic improvement can be performed on the antiviral infection ability of the plants by using NbPPO-related biological products.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

The present invention relates to the use of triazole compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The invention discloses application of naringin in resisting potato virus Y of plants, and belongs to the technical field of biology. According to the invention, a candidate compound naringin is successfully found from a ZINC database by adopting a virtual screening method and taking PVYHC-Pro as a target, and then the inhibition effect of the naringin on PVY is detected by adopting methods such as protein expression and purification, trace thermophoresis and plant living body experiments. Experiments show that after the naringin is sprayed to the nicotiana benthamiana, obvious green fluorescence does not appear on system leaves on the 6th day, and a small amount of fluorescence appears on the 8th day and the 10th day. The nicotiana benthamiana sprayed by contrast DMSO shows a large amount of fluorescence in the 6th day, and the fluorescence is continuously enhanced in the 8th and 10th days. Therefore, the naringin has good inhibitory activity on PVY, that is, the naringin can target PVY HC-Pro protein to interact with the PVY HC-Pro protein, and the accumulation of PVY (potato Y virus) in plants is inhibited.

The present invention relates to the use of benzo[B][1,8]naftiridine-based compounds for controlling potato Y virus, and the agrochemical composition according to the present invention can be usefully utilized for controlling potato Y virus.

The application belongs to the field of agricultural chemicals, and particularly relates to a compound with anti-potato virus Y virus activity and a preparation method and application thereof. 16 H 23 N5O7, the compound provided by the application has good inhibiting effect on viruses of the potato virus Y, such as potato virus Y, sugarcane mosaic virus and turnip mosaic virus, and can be used for preparation of anti-plant virus pesticides, and has wide application prospect.

This invention discloses a hybridoma cell line secreting monoclonal antibody PVY-2, its monoclonal antibody, and its applications. The hybridoma cell line 4F1B2G11 secretes a monoclonal antibody against potato virus Y. The preservation number of the hybridoma cell line is CCTCC NO: C2022281. The monoclonal antibody PVY-2 has a specific immunoreactivity with the coat protein of potato virus Y. The nucleotide sequence of the heavy chain variable region of monoclonal antibody PVY-2 is shown in SEQ ID NO.4, and the nucleotide sequence of the light chain variable region is shown in SEQ ID NO.5. The sensitivity of the direct ELISA method for detecting potato virus Y-infected leaves reaches a 1:5120 dilution. The monoclonal antibody PVY-2 can also be used to prepare ELISA kits, colloidal gold kits, or nano-enzyme kits, enabling rapid, sensitive, economical, and accurate detection of potato virus Y disease.

The application discloses an application of Terminalia chebula gallae in resisting potato virus Y, and belongs to the technical field of biotechnology. The application takes the potato virus Y VPg protein as a target, and successfully obtains the candidate compound Terminalia chebula gallae through virtual screening, and verifies high affinity binding of the candidate compound with the potato virus Y VPg and an antiviral effect from three levels of a molecular level, an in-vitro interaction and a living body level. The results show that the Terminalia chebula gallae can effectively inhibit the spread and infection of the potato virus Y on plants, and relieve the plant disease process. The application provides a theoretical basis and an experimental basis for antiviral drug design based on the VPg target, and lays a foundation for subsequent structure optimization of the Terminalia chebula gallae, development of green pesticides and in-depth analysis of an action mechanism.

The invention discloses a botanical antiviral preparation as well as a preparation method and application thereof, and belongs to the technical field of preparation of botanical antiviral agents. The invention discloses a botanical antiviral preparation, which is prepared from the following components in percentage by weight: 5 to 9 percent of (12R, 13R)-8, 12-epoxy-14-labden-13-ol, 20 to 30 percent of ethanol, 15 to 25 percent of sucrose ester, 6 to 8 percent of lignosulfonate, 0.2 to 0.4 percent of magnesium aluminum silicate, 2 to 4 percent of glycerol and the balance of deionized water. The botanical antiviral preparation disclosed by the invention is high in antiviral activity, the prevention effect of the botanical antiviral preparation on tobacco mosaic virus, potato Y virus and cucumber mosaic virus is obviously superior to that of botanical pesticide amino-oligosaccharin, and the preparation does not contain any toxic organic solvent and is safe to the environment, people and livestock.