The invention belongs to the technical field of
plant genetic engineering, and particularly relates to application of an NtSGRL1-related
biological product in tobacco antivirus, and the
nucleotide sequence of the NtSGRL1 is shown as SEQ ID NO.1. The invention also relates to an application of the NtSGRL1-related
biological product in tobacco antivirus. According to the invention, a
CRISPR-Cas9 mediated common tobacco NtSGRL1 knockout strain Ntsgrl1 is created through genetic transformation, and four main tobacco viruses of
tomato yellow leaf curl virus,
tobacco mosaic virus, potato Y
virus and
tomato spotted wilt virus are respectively adopted to inoculate the obtained Ntsgrl1 function
deletion mutant strain.
Disease resistance identification results show that compared with wild and overexpression strains, the Ntsgrl1
mutant strain shows broad-spectrum and significantly enhanced resistance to four tested viruses, which is specifically embodied in that the
system symptom development is significantly inhibited, and the
virus accumulation in the
plant is significantly reduced.