472 results about "Virus diseases" patented technology

The invention discloses a melon vegetable virus disease identification method and system constructed based on a sensor array. The method comprises the following steps: generating a dynamic thermodynamic diagram by collecting insect activity data of a target planting area, constructing a virus disease propagation distribution diagram in combination with an entomophilous propagation probability model, and deploying an initial sensor array according to the virus disease propagation distribution diagram; plant physiological parameters and environmental data are obtained in real time through multiple sensors, a virus disease recognition model fused with entomophilous activity characteristics is constructed, and early diagnosis of diseases is achieved; and evaluating the efficiency of the sensor array based on a recognition result, and dynamically adjusting the array layout by adopting a self-adaptive optimization algorithm to form an optimized network with balanced monitoring precision and resource efficiency. The virus disease detection sensitivity and accuracy are remarkably improved, the monitoring cost is reduced, and a digital solution is provided for prevention and control of diseases of melon crops.

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

The invention discloses an anti-pestivirus broad-spectrum neutralizing monoclonal antibody and application thereof, and belongs to the field of immune globulin. The anti-pestivirus monoclonal antibody or the antigen binding fragment thereof comprises a heavy chain variable region (SEQ ID No.1) and a light chain variable region (SEQ ID No.3). The monoclonal antibody provided by the invention can identify hog cholera virus and all known plague virus members (BVDV1, BVDV2, BDV, HoBiPeV, AydinPeV and OVPV) capable of infecting cattle and sheep at present, has good neutralizing ability, and can be prepared into a diagnostic product for plague virus antigen-antibody detection. The monoclonal antibody and a diagnostic product developed based on the monoclonal antibody can be used for serological diagnosis and immune effect evaluation of pestivirus, pestivirus pathogen detection of experimental serum products and the like, and have wide application prospects in the fields of clinical diagnosis and the like.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention relates to the field of aquaculture, in particular to a preparation method and application of a larimichthys crocea iridovirus recombinant live vector vaccine. According to the invention, replication-deficient type 5 adenovirus is selected as a target gene expression vector, a recombinant adenovirus vector comprising main capsid protein of iridovirus is constructed, recombinant virus is obtained, and the safe and effective large yellow croaker iridovirus disease recombinant live vector vaccine is created. According to the vaccine, the larimichthys crocea is immunized in a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 12.0%, and the CPM of the fish without being immunized by the vaccine is 62.0%. In addition, the RPS of the inoculated fish and the RPS of the empty carrier control group fish are 80.65% and 9.68% respectively. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

The invention discloses a short peptide PRP1 as well as a preparation method and application thereof. Belongs to the technical field of gene engineering. According to the application, an oligopeptide which is derived from unknown protein and can regulate and control the resistance of tobacco to the potato Y virus disease is obtained through research and identification, the oligopeptide is named as PVY residual peptide (PRP1), the amino acid sequence of the oligopeptide is ATKHIVGSIVAS, the oligopeptide PRP1 is prepared through a chemical synthesis method, and the oligopeptide is injected into the tobacco at the seedling stage, so that the PVY virus quantity can be effectively reduced, and the resistance of the tobacco to the potato Y virus disease can be improved.

The invention provides a compound disinfectant for preventing and treating aquaculture virus diseases and a preparation method thereof. The composite disinfectant is prepared by introducing polyhexamethylene guanidine or a salt thereof, a quaternary ammonium salt compound, metal nanoparticles and an auxiliary agent into a water system as main raw materials, and is prepared by the following steps: adding a quaternary ammonium salt compound solution and a penetrating agent into a polyhexamethylene guanidine or salt solution thereof, and uniformly stirring and mixing to obtain a mixed solution A; slowly dropwise adding the metal nanoparticle dispersion liquid into the mixed solution A, starting ultrasonic treatment at the same time, and uniformly stirring and mixing to obtain a mixed solution B; dropwise adding the pH regulator solution into the mixed solution B, regulating the pH, adding the preservative solution, supplementing water, uniformly mixing, standing and curing to obtain the composite disinfectant. The compound disinfectant aims at achieving synergistic interaction, finally achieves the comprehensive prevention and treatment effects of being efficient, low in toxicity, broad in spectrum and not prone to inducing drug resistance, and particularly achieves efficient and safe prevention and control on fish, shrimp and crab virus diseases so as to meet the urgent requirement for healthy aquaculture.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention provides a tobacco virus disease detection method based on multispectrum and improved BPNN, and belongs to the technical field of electrical digital data processing. Tobacco vegetation data is collected through a multispectral unmanned aerial vehicle, 19 vegetation indexes and a feature set thereof are calculated, and classification is carried out by adopting an improved BPNN model. Feature optimization equations including feature significance, time sequence correlation, space consistency and a comprehensive contribution degree equation are introduced innovatively, and multi-dimensional optimization of vegetation index features is achieved. Through division of regular features and irregular features, a correlation mechanism of feature changes and environmental factors is established in combination with a feature time correlation change rate. And finally, based on the characteristic comprehensive contribution degree score, selecting the optimal characteristic variable to perform binary and ternary classification, thereby realizing accurate identification and degree evaluation of the tobacco virus disease. The technical problem that in the prior art, time sequence change rules, spatial correlation and environmental factor influences of multispectral features cannot be effectively fused to accurately recognize tobacco virus diseases is solved.

The invention provides a larimichthys crocea iridovirus inactivated vaccine as well as a preparation method and application thereof, and belongs to the technical field of preparation of viral vaccines. The larimichthys crocea iridovirus inactivated vaccine comprises an inactivated larimichthys crocea iridovirus and an adjuvant, and the strain of the larimichthys crocea iridovirus is an FD201807 strain. After the larimichthys crocea iridovirus inactivated vaccine is injected, the phenomena of ingestion and abnormal behaviors of fishes are avoided, the death phenomenon is avoided, the safety is high, the relative immune protection rate of the inactivated vaccine to immunized fishes is 51.20% or above, the immune effect is good, and the larimichthys crocea iridovirus inactivated vaccine can be applied to prevention and treatment of larimichthys crocea iridovirus diseases. The preparation method of the larimichthys crocea iridovirus inactivated vaccine is simple and convenient, easy to operate, low in cost and suitable for industrial production.

The invention discloses an alternative virus infection model capable of being used for simulating human Marburg virus disease symptoms under BSL-2 as well as an establishment method and application of the alternative virus infection model, and belongs to the technical field of biology. According to the invention, firstly, a recombinant vesicular stomatitis virus (VSV-MARV / GP) for expressing Marburg virus glycoprotein is constructed, and the VSV-MARV / GP is used as an alternative virus to cause lethal infection of a narrative hamster. The pathogenicity of the VSV-MARV / GP has species specificity, age correlation, gender correlation and attack pathway dependency. After infection of VSV-MARV / GP, female narrative hamsters of 5 weeks old show weight loss, multiple organ failure, high virus load and other symptoms and develop into serious systemic diseases similar to those observed by human MARV patients, and all animals die within two to three days after infection. The model provided by the invention can be used as an effective tool for carrying out rapid preclinical evaluation on medical countermeasures of MARV under the BSL-2 condition.

The present disclosure relates to field of lyophilized / freeze-dried viral vaccine composition and methods for manufacturing and obtaining the vaccine composition comprising live attenuated flavivirus as vaccine antigen selected from a group of yellow fever virus, dengue virus, Japanese encephalitis (JE) virus, Kunjin virus, West Nile (WN) virus, tick-borne encephalitis (TBE) virus, St. Louis encephalitis virus, Murray Valley encephalitis virus, Zika virus vaccine antigen; and stabilizers comprising sugar or sugar alcohol; amino acids; lactalbumin hydrolysate and gelatin. Post reconstitution, the composition preserves the desired characteristics of the virus, including immunogenicity and stability. The composition of the present disclosure can be safely administered subcutaneously or intramuscularly such that the vaccine composition induces complete immune response to yellow fever virus infectious agent and meets the criterion for the seroprotection for the said immunogenic components comprising yellow fever virus.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The present disclosure provides recombinant adeno-associated virus (AAV) virions with altered capsid protein, where the recombinant AAV (rAAV) virions exhibit greater ability to cross barriers between intravitreal fluid and retinal cells, and thus greater infectivity of a retinal cell compared to wild-type AAV, and where the rAAV virions comprise a heterologous nucleic acid. The present disclosure provides methods of delivering a gene product to a retinal cell in an individual.

Provided herein are immunogenic compositions comprising a chimeric influenza A virus HA and a CpG oligonucleotide adjuvant. Also provided herein are immunogenic compositions comprising an inactivated influenza A virus described herein or inactivated split influenza A virus described herein and a CpG oligonucleotide adjuvant described herein, wherein the inactivated influenza A virus or inactivated split influenza A virus comprises a chimeric hemagglutinin described herein. The immunogenic compositions may be used in a sequential immunization regimen. Also provided herein are methods of immunizing a subject against influenza virus disease (e.g., influenza virus disease caused by influenza A virus, such as, e.g., group 2 influenza A virus) using an immunogenic composition described herein. Further, provided herein are methods of preventing influenza virus disease (e.g., influenza virus disease caused by influenza A virus, such as, e.g., group 2 influenza A virus) in a subject using an immunogenic composition described herein.

The invention discloses a tomato disease-resistant gene mutant and application thereof in prevention and treatment of ToBRFV, and belongs to the technical field of biological prevention and treatment of viral diseases. According to the invention, the 1927th nucleotide of the coding region sequence of the tomato Tm-22 gene is mutated from G to A; or the 643th amino acid of the LRR structural domain of the tomato Tm-22 gene encoding protein is mutated from glycine to arginine, so that the accumulation level of ToBRFV capsid protein (CP) can be obviously reduced. The gene (named as Tm-22-Mut5) not only can be used as a new ToBRFV resistance gene, but also retains the resistance to TMV, ToMV and ToMMV. The method comprises the following steps: carrying out combined mutation on Tm-22-Mut5 and a Tm-22-Mut3-1 mutant (tyrosine at the 767th site in an LRR structural domain is mutated into phenylalanine) screened in an earlier stage, so as to obtain a mutant Tm-22-Mut6. According to the present invention, the analysis results show that the accumulation level of the ToBRFV capsid protein can be significantly reduced by using the Tm-22-Mut6; compared with a mutant pFGCTm-22-Mut3-1 obtained through early research and screening and a newly obtained mutant Tm-22-Mut5, the resistance of the Tm-22-Mut6 to the ToBRFV is further improved, and the resistance of the Tm-22-Mut6 to the ToBRFV is further improved.

The invention discloses application of GSK2606414 in preparation of a medicine for inhibiting duck tembusu virus infection, and belongs to the technical field of veterinary biological products. The research finds that the GSK2606414 can effectively inhibit the replication of the duck tembusu virus for the first time, provides a new therapeutic drug for the prevention and treatment of the duck tembusu virus, and is of great significance to the actual production of the poultry breeding industry.

A lighting fixture containing both visible light sources and UV light sources is disclosed herein. In one embodiment, the fixture is an overhead fixture. UV light can sanitize / disinfect the surrounding environment over a period of about 30-45 minutes. In this way, viruses, germs, bacteria, mold, and the like can be destroyed to clean the air and / or surfaces (e.g., furniture) of the room exposed to UV light. Accordingly, embodiments of the present invention include a physical input device, such as a wall-mounted button, switch, or wireless device, that can safely and efficiently activate a lighting assembly to produce UV light in a way that protects occupants from unintentional exposure to UV light. This configuration is especially useful in situations where the use of UV light is regulated, such as in a hospital setting.

The invention discloses an application of an NbFKBP13 gene in resisting tomato yellow leaf curl virus. Belongs to the technical field of plant disease control. The overexpression NbFKBP13 transgenic nicotiana benthamiana which can be stably inherited is obtained through a gene engineering technology. Phenotypic observation shows that the NbFKBP13 transgene has no significant influence on the growth and development of nicotiana benthamiana; it is found through artificial inoculation of TYLCV that after overexpression of the NbFKBP13 gene, the resistance of nicotiana benthamiana to TYLCV can be significantly enhanced. The research provides an important gene pool and a new germplasm resource for virus disease resistance breeding.

Methods of reducing hepatitis delta virus (HDV) viral loads in a patient are provided. In some embodiments, the method comprises treating the patient with lonafarnib-ritonavir co-therapy. In some embodiments, the method further comprises treating the patient with an interferon.

The invention provides a probe library for high-throughput detection of mouse and hamster related species-specific viruses in a CHO cell library and application of the probe library. The probe library is used for detecting a mouse pox virus, a hantavirus, a K virus, a lactic dehydrogenase virus, a lymphocytic choriomeningitis virus, a mouse microvirus, a mouse adenovirus, a mouse cytomegalovirus, a mouse encephalomyelitis virus, a mouse hepatitis virus, a mouse rotavirus, a mouse pneumovirus, a multiple oma virus, a reovirus type 3 and a sendai virus; sV5 and thymoviruses and variant viruses thereof. The high-throughput sequencing method based on the probe library can be used for distinguishing viral nucleic acid with infection risk and quickly checking whether a sample is polluted by specific live viruses or not. The detection limit reaches the level of a traditional PCR method, the capability of detecting all known variants of to-be-detected species-specific viruses is kept, 17 viruses can be detected only by implementing one detection item, and the method has the advantages of high efficiency, rapidness and high sensitivity.

The invention relates to the technical field of plant disease control, in particular to application of a quinazoline carboxylic acid compound in plant virus resistance. The invention discovers that 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid (DHCA) has the effect of resisting plant viruses for the first time. In the specific embodiment of the invention, the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid is used for preventing and treating the cucumber virus disease and the pepper virus disease, and the result shows that the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid and the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid are used for preventing and treating the cucumber virus disease and the pepper virus disease. And the composition has a good control effect by combining the 2, 2-a] quinazoline-3a (1H)-carboxylic acid with wuyiencin.

The invention provides a method for removing capsicum light mottle viruses in capsicum seeds. The method comprises the following steps: (1) carrying out protease hydrolysis treatment on the capsicum seeds by adopting a protease aqueous solution capable of hydrolyzing virus protein shells; and (2) the pepper seeds treated in the step (1) are subjected to light irradiation in a solution containing a photosensitizer to excite the photosensitizer, so that the pepper seeds without the pepper mild mottle viruses are obtained, and the photosensitizer is a visible light excited photosensitizer. Compared with an existing PMMoV virus resisting method, the virus removing method has the advantages that PMMoV viruses of the pepper seeds can be effectively removed, the germination potential and the germination rate of the pepper seeds are not reduced, and the method is more efficient, economical, environmentally friendly and convenient to popularize; the method can promote the prevention and control of pepper virus diseases and the breeding of virus-free pepper seedlings, and guarantee the long-distance transportation of seeds.

The invention relates to the technical field of tobacco virus disease distinguishing, and discloses a tobacco virus disease remote sensing distinguishing method and system based on multi-source data, and the method comprises the following steps: carrying out chlorophyll content and leaf area index determination on a tobacco sample to obtain chlorophyll characteristics and leaf area characteristics; fusing the spectral wavelet features, the chlorophyll features and the leaf area features, and preprocessing the fused data to form a data set; calling a historical tobacco classification database, and constructing a tobacco virus disease distinguishing model; inputting the collected spectral wavelet features, chlorophyll features and leaf area features into a tobacco virus disease classification model for classification and identification; and evaluating data classified by the tobacco virus disease classification model, and adjusting the tobacco virus disease classification model according to an evaluation result. The system comprises a tobacco sample collecting module, a distinguishing model constructing module and a distinguishing evaluating module. According to the invention, accurate distinguishing and identification of tobacco virus diseases in a complex field environment are realized.

The present invention relates to a composition for treatment of coronavirus disease 2019 (COVID-19) containing taurodeoxycholic acid (TDCA) or a pharmaceutically acceptable salt thereof as an active ingredient. Specifically, an early full recovery effect has been confirmed through the efficacy of treating clinical symptoms caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, alleviating inflammation, and inhibiting inflammatory cytokines using a pharmaceutically acceptable salt of taurodeoxycholic acid, and thus the taurodeoxycholic acid or a pharmaceutically acceptable salt thereof can be used as an active ingredient in a composition for prevention or treatment of lower respiratory tract infectious diseases, including coronavirus disease 2019.

The invention provides a largemouth bass frog iridovirus, a vaccine prepared from the largemouth bass frog iridovirus and application of the largemouth bass frog iridovirus, and belongs to the technical field of biological The largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth The strain and the vaccine prepared from the strain can effectively prevent attack of the largemouth bass frog iridovirus, and have relatively good protection force on the largemouth bass frog iridovirus disease.

In some aspects, cardiac-specific expression cassettes are provided herein. In some aspects, provided herein is an expression cassette comprising a polynucleotide sequence encoding a gene product for therapy of a heart disease, wherein the polynucleotide sequence is operably linked to promoter (e.g., a cardiac-specific promoter), and optionally an enhancer (e.g., a cardiac-specific enhancer). In some aspects, the disclosure provides recombinant adeno-associated virus (rAAV) virions, comprising a capsid protein and a viral genome comprising an expression cassette comprising a polynucleotide sequence encoding a therapeutic gene product, e.g., dwarf open reading frame (DWORF) polypeptide, operably linked to a promoter, the expression cassette flanked by inverted terminal repeats. The disclosure further provides pharmaceutical compositions and methods of treating or preventing heart disease.

The invention relates to the technical field of biological medicine, and discloses a titer determination method of a specific immune globulin neutralizing antibody, which comprises the following steps: step 1, preparing a cell maintenance fluid; 2, preparation of a virus solution for neutralization: diluting viruses (cytomegalovirus, syncytial virus and EV71 virus) to 100CCID50 by using a diluent according to the virus titer; step 3, diluting the test sample: carrying out double series dilution on the intravenous injection human immune globulin, the tetanus human immune globulin, the rabies human immune globulin and the human immune globulin until no cytotoxicity exists; 4, diluting a standard substance; 5, virus back dripping; step 6, neutralizing viruses; step 7, preparing cells for the test; step 8, infection; and 9, judging a result. According to the present invention, the accurate detection on the titer of the neutralizing antibody of the specific immune globulin can be achieved, and the cytotoxicity influence of the test sample and the fringe effect during the detection process can be removed so as to ensure the detection accuracy.

Provided are a replicable RNA molecule and uses thereof, the present invention relates to a replicable RNA molecule comprising, from the 5'end to the 3 'end, a 5' cap, a 5 'UTR, an open reading frame encoding an RNA replicase, a promoter, a sequence of interest, a 3' UTR, and a poly (A) tail wherein the RNA replicase is capable of amplifying the replicable RNA molecule and an RNA molecule containing the sequence of interest and the 3 'UTR wherein the RNA replicase is derived from the group consisting of the group consisting of the MosdPedras virus, MDPV, EVEV, Riogro virus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus, Mycovirus and Mycovirus. RnV, Muslim virus, MUCV, Highland J virus, PIXV, TROV, Cabassu virus, TONV, Bebaru virus, BEBV, Formun virus, GETV, or Nnumu virus, and NDUV can be used in the treatment of a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, a disease, and a disease.

The invention discloses a screening method of application of a mysorethorn cold mixture to treatment of respiratory syncytial virus. According to the method, network pharmacology and a molecular docking technology are combined, and active ingredients, action targets and action mechanisms of the mysorethorn cold mixture for treating respiratory syncytial virus diseases are explored; an experimental basis is provided for treating the respiratory syncytial virus disease by the mysorethorn cold mixture, and the mysorethorn cold mixture is of great significance in promoting clinical application of traditional Chinese medicines in treating the respiratory syncytial virus disease.