263 results about "Virus diseases" patented technology

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention relates to the field of aquaculture, in particular to a preparation method and application of a larimichthys crocea iridovirus recombinant live vector vaccine. According to the invention, replication-deficient type 5 adenovirus is selected as a target gene expression vector, a recombinant adenovirus vector comprising main capsid protein of iridovirus is constructed, recombinant virus is obtained, and the safe and effective large yellow croaker iridovirus disease recombinant live vector vaccine is created. According to the vaccine, the larimichthys crocea is immunized in a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 12.0%, and the CPM of the fish without being immunized by the vaccine is 62.0%. In addition, the RPS of the inoculated fish and the RPS of the empty carrier control group fish are 80.65% and 9.68% respectively. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

The invention discloses a short peptide PRP1 as well as a preparation method and application thereof. Belongs to the technical field of gene engineering. According to the application, an oligopeptide which is derived from unknown protein and can regulate and control the resistance of tobacco to the potato Y virus disease is obtained through research and identification, the oligopeptide is named as PVY residual peptide (PRP1), the amino acid sequence of the oligopeptide is ATKHIVGSIVAS, the oligopeptide PRP1 is prepared through a chemical synthesis method, and the oligopeptide is injected into the tobacco at the seedling stage, so that the PVY virus quantity can be effectively reduced, and the resistance of the tobacco to the potato Y virus disease can be improved.

The invention provides a compound disinfectant for preventing and treating aquaculture virus diseases and a preparation method thereof. The composite disinfectant is prepared by introducing polyhexamethylene guanidine or a salt thereof, a quaternary ammonium salt compound, metal nanoparticles and an auxiliary agent into a water system as main raw materials, and is prepared by the following steps: adding a quaternary ammonium salt compound solution and a penetrating agent into a polyhexamethylene guanidine or salt solution thereof, and uniformly stirring and mixing to obtain a mixed solution A; slowly dropwise adding the metal nanoparticle dispersion liquid into the mixed solution A, starting ultrasonic treatment at the same time, and uniformly stirring and mixing to obtain a mixed solution B; dropwise adding the pH regulator solution into the mixed solution B, regulating the pH, adding the preservative solution, supplementing water, uniformly mixing, standing and curing to obtain the composite disinfectant. The compound disinfectant aims at achieving synergistic interaction, finally achieves the comprehensive prevention and treatment effects of being efficient, low in toxicity, broad in spectrum and not prone to inducing drug resistance, and particularly achieves efficient and safe prevention and control on fish, shrimp and crab virus diseases so as to meet the urgent requirement for healthy aquaculture.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention discloses application of GSK2606414 in preparation of a medicine for inhibiting duck tembusu virus infection, and belongs to the technical field of veterinary biological products. The research finds that the GSK2606414 can effectively inhibit the replication of the duck tembusu virus for the first time, provides a new therapeutic drug for the prevention and treatment of the duck tembusu virus, and is of great significance to the actual production of the poultry breeding industry.

A lighting fixture containing both visible light sources and UV light sources is disclosed herein. In one embodiment, the fixture is an overhead fixture. UV light can sanitize / disinfect the surrounding environment over a period of about 30-45 minutes. In this way, viruses, germs, bacteria, mold, and the like can be destroyed to clean the air and / or surfaces (e.g., furniture) of the room exposed to UV light. Accordingly, embodiments of the present invention include a physical input device, such as a wall-mounted button, switch, or wireless device, that can safely and efficiently activate a lighting assembly to produce UV light in a way that protects occupants from unintentional exposure to UV light. This configuration is especially useful in situations where the use of UV light is regulated, such as in a hospital setting.

The invention relates to the technical field of plant disease control, in particular to application of a quinazoline carboxylic acid compound in plant virus resistance. The invention discovers that 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid (DHCA) has the effect of resisting plant viruses for the first time. In the specific embodiment of the invention, the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid is used for preventing and treating the cucumber virus disease and the pepper virus disease, and the result shows that the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid and the 1, 5-dioxo-2, 3, 4, 5-tetrahydropyrrolo [1, 2-a] quinazoline-3a (1H)-carboxylic acid are used for preventing and treating the cucumber virus disease and the pepper virus disease. And the composition has a good control effect by combining the 2, 2-a] quinazoline-3a (1H)-carboxylic acid with wuyiencin.

The present invention relates to a composition for treatment of coronavirus disease 2019 (COVID-19) containing taurodeoxycholic acid (TDCA) or a pharmaceutically acceptable salt thereof as an active ingredient. Specifically, an early full recovery effect has been confirmed through the efficacy of treating clinical symptoms caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, alleviating inflammation, and inhibiting inflammatory cytokines using a pharmaceutically acceptable salt of taurodeoxycholic acid, and thus the taurodeoxycholic acid or a pharmaceutically acceptable salt thereof can be used as an active ingredient in a composition for prevention or treatment of lower respiratory tract infectious diseases, including coronavirus disease 2019.

The invention discloses application of TBR2 protein as well as a coding gene and a molecular marker thereof in resisting tomato brown wrinkled fruit virus diseases, and belongs to the technical field of gene engineering. According to the invention, a novel anti-disease gene TBR2 for resisting the tomato brown wrinkled fruit virus disease is identified on a chromosome 4 of tomatoes; the disease-resistant gene has stable disease resistance, and the resistance of the variety to the tomato brown crinkled fruit virus disease can be obviously improved by backcrossing and transbreeding the disease-resistant gene into a sensitive cultivar. Based on the anti-disease gene TBR2, the invention further develops molecular markers related to the tomato brown wrinkled fruit virus disease resistance, and the molecular markers can be used for identifying plant materials resistant to the tomato brown wrinkled fruit virus disease and cultivating plant varieties resistant to the tomato brown wrinkled fruit virus disease. The method is of great significance in accelerating the breeding process of disease-resistant tomatoes.

The invention discloses an anti-disease gene, an anti-disease protein, a molecular marker and application of tomato brown crinkled fruit virus disease, and belongs to the technical field of gene engineering. According to the invention, through a map-based cloning method, a new anti-disease gene TBR3 of the tomato brown fruit wrinkling virus disease is identified in a tomato chromosome 2 for the first time. The TBR3 gene is over-expressed in a plant body, so that the disease resistance of the plant to the tomato brown crinkled fruit virus disease is remarkably improved. Aiming at the disease-resistant gene TBR3, the invention also designs a molecular marker related to tomato brown crinkled fruit virus resistance, and tomato plants carrying a disease-resistant genotype and a susceptible genotype can be accurately distinguished, so that the molecular marker can be widely applied to early disease-resistant breeding and screening, and the problems of difficult early judgment and low breeding efficiency in a breeding process are solved.

The invention relates to a preparation method of a Vero cell influenza virus vaccine, and belongs to the technical field of virus culture. In the culture of the influenza virus, red algae protein and soybean protein hydrolysate are added into a culture medium at the same time, the red algae protein and soybean protein hydrolysate are subjected to low-temperature ammonium sulfate precipitation and PD-10 desalination column purification, natural conformation is kept, integrin-FAK-Src and IGF-1R signal channels can be activated, and cell adhesion and proliferation are promoted; the latter contains soybean protein isolate, polysaccharide and RGD motif peptide, and can form an adhesion film on a substrate and mediate adhesion through integrin. According to the invention, the Vero cells and the recombinant plasmid are combined to improve cell adherence, metabolic homeostasis and oxidation resistance, so that the Vero cells grow at high density and efficiently replicate influenza viruses under a serum-free condition, the virus titer (TCID50) reaches 8.5 log10 / mL, and the Vero cells and the recombinant plasmid are suitable for industrial vaccine production.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

A biocontrol agent of the present invention, comprising one or more of the selected, isolated bacteria disclosed herein, is useful in methods of protecting plants against disease or damage by plant pathogens. A method comprises applying a biocontrol agent to plants, plant seeds, or media or soil surrounding plants provide disease protection to plants or plants produced from the contacted plant seeds. Biocontrol agents of the present invention are useful in horticulture, aquaculture, aeroculture, hydroponic culture, agriculture and all types of plant growing conditions to protect plants from a variety of plant bacterial, fungal, and viral diseases. The compositions and methods of the present invention are effective against plant pathogens.

Provided herein are immunogenic compositions comprising a mosaic influenza B virus hemagglutinin (HA) and a CpG oligonucleotide adjuvant. Also provided herein are immunogenic compositions comprising an inactivated influenza B virus described herein or inactivated split influenza B virus described herein and a CpG oligonucleotide adjuvant described herein, wherein the inactivated influenza B virus or inactivated split influenza B virus comprises a mosaic HA described herein. The immunogenic compositions may be used in a sequential immunization regimen. Also provided herein are methods of immunizing a subject against influenza virus disease (e.g., influenza virus disease caused by influenza B virus) using an immunogenic composition described herein. Further, provided herein are methods of preventing influenza virus disease (e.g., influenza virus disease caused by influenza B virus) in a subject using an immunogenic composition described herein.

The invention discloses insect lysozyme capable of preventing and treating rice virus diseases and application of the insect lysozyme. According to the invention, three pieces of lysozyme (RdLYZI1, RdLYZC1 and RdLYZC2) with remarkable inhibitory activity on various rice viruses are innovatively excavated from the body of a natural transmission vector insect of the viruses, and the amino acid sequences of the lysozyme are shown as SEQ ID NO.13, SEQ ID NO.14 and SEQ ID NO.15. The invention further discloses a preparation method of the lysozyme. Experimental verification shows that the lysozyme RdLYZI1, the lysozyme RdLYZC1 and the lysozyme RdLYZC2 can effectively inhibit replication and accumulation of various rice viruses in plants and vector insects, have a remarkable prevention and treatment effect on related virus diseases and relieve plant growth obstacles caused by the viruses. The invention provides a novel biological prevention and control strategy which is environment-friendly and high in targeting property for green prevention and control of rice virus diseases, and has an important application prospect.

Herpesviridae glycoprotein B (gB) polypeptides comprising a modified ectodomain is stabilized in the prefusion state, enabling development of inhibitors and vaccines directed against these viral pathogens. Modifications to DI, DII, and DV subdomains are important to stabilization of gB in the prefusion state, and additional modifications result in a further improved stabilization of gB in the prefusion state. The gB can be derived from an Epstein Barr Virus (EBV), a Human Cytomegalovirus (CMV), a Human Herpesvirus 6 (HHV6), a Herpes Simplex Virus 1 (HSV1), or a Varicella Zoster Virus (VZV). Polypeptides, nucleic acid constructs, compositions, and methods of using same to elicit an immune response are described.

The invention relates to application of RING type rice E3 ubiquitin ligase OsCOP1 in prevention and control of rice virus diseases, and belongs to the field of plant genetic engineering technology and disease prevention and control.

The invention discloses a recombinant salmonella choleraesuis nuclease regulation vector as well as a construction method and application thereof. After oral administration, the recombinant salmonella choleraesuis nuclease regulatory vector rSC0140 enters host immune cells, c-di-AMP is released, a host STING pathway is activated, and a broad-spectrum antiviral effect can be achieved; the recombinant strain rSC0140 is proved to be capable of activating STING pathways in macrophages and mice, causing antiviral states of the macrophages and the mice and resisting challenge of various influenza viruses; the recombinant strain has broad-spectrum antiviral efficacy and is suitable for preventing and treating various virus diseases clinically.

Vaccine compositions for protecting a subject against diseases caused by viral pathogens of the Picornaviridae family are disclosed. These vaccines comprise nucleic acids encoding the capsid polyprotein (P1 polyprotein) mixed with the viral 3C protease that processes the P1 polyprotein, and encapsulated within nanoparticle carriers. Methods of preventing, reducing, inhibiting, or delaying the symptoms of an infection caused by a viral pathogen, or of inducing an immune response against a viral pathogen in a subject are provided. Methods of making the vaccines are also described.

This invention discloses a method for inhibiting Tamdy virus infection and the use of TCP1 inhibitors. Specifically, this invention discloses a method for inhibiting Tamdy virus (TAMV) infection of host cells, the method comprising the step of reducing or inhibiting the expression level and / or activity of the TCP1 gene or its encoded protein in host cells, wherein the reduction or inhibition of the expression level and / or activity of the TCP1 gene or its encoded protein is achieved by methods selected from the group consisting of: gene mutation, gene knockout, gene interruption, RNA interference technology, gene editing technology, introduction of inhibitors of genes or proteins, or combinations thereof.

The invention discloses primers, a kit and a method for detecting four viruses in lentinus edodes. According to the invention, LAMP (loop-mediated isothermal amplification) primer groups are respectively designed and screened according to four viruses in the lentinula edodes, and the four viruses are respectively a Lentinula edodes dominant virus 1 virus, a Lentinula edodes dominant virus HKB virus, a Lentinula edodes dominant gene-like virus 1-Hunong-1 virus and a Lentinula edodes dominant virus 5 virus. The detection system is micro-quantized, the local concentration of the target sequence is improved from the side, the detection limit is greatly improved, and the lower detection limit can reach 10 copy / microliter.

The present invention relates to a pharmaceutical composition for preventing or treating coronavirus disease 2019, and more specifically to a pharmaceutical composition for preventing or treating coronavirus disease 2019 found by drug repositioning technology using drug virtual screening technology. The pharmaceutical composition for preventing or treating coronavirus disease 2019 according to the present invention is a composition obtained by finding new uses for drugs, which have already been proven effective, for preventing or treating coronavirus disease 2019 by drug repositioning technology. The pharmaceutical composition is useful because it has significantly lower side effects than new drugs and can be rapidly applied to clinical practice.

The invention provides an oligopeptide with a fish virus resisting effect, and the oligopeptide is used as a feed additive or a liquid protein preparation for injection to kill viruses or inhibit the proliferation of the viruses, so that the treatment of the fish virus diseases is realized. The amino acid sequence of the oligopeptide is SEQ ID NO: 1. The Pt5-1c oligopeptide provided by the invention reduces the cytopathic effect degree of GCRV virus infection on EPC, and shows that the Pt5-1c oligopeptide has an important antiviral effect. By adding the Pt5-1c oligopeptide into the feed or injecting the Pt5-1c oligopeptide into the fish, the purpose of preventing and treating viral diseases of the fish can be achieved.

The invention relates to the technical field of aquatic product culture, and particularly discloses application of zinc in preparation of a product for preventing and treating siniperca chuatsi rhabdovirus infection. By adding a proper amount of zinc ions into a mandarin fish cell culture system or culture environment, the application of zinc after infection in forms of zinc sulfate and the like can significantly inhibit protein expression and virus proliferation of the mandarin fish rhabdovirus, and has no toxic or side effect on host cells within a certain concentration range. The novel nutritional immune prevention and control strategy which is safe, economical and easy to operate provides an effective means for prevention and control of siniperca chuatsi virus diseases, and has important application prospects and economic values.

The invention discloses a method for detecting RPA (recombinase polymerase amplification) combined Cas12b of strawberry vein banding virus in strawberry leaves and a special kit thereof. The detection method comprises the following steps: extracting total DNA of a strawberry leaf sample to be detected; carrying out RPA (recombinase polymerase amplification) combined Cas12b amplification by taking DNA (deoxyribonucleic acid) as a template and utilizing a primer and reagent combination; and fluorescence observation or test strip detection can be carried out on a reaction product by using different probes so as to judge whether a sample contains the strawberry vein banding virus or not. The primer combination comprises an RPA (recombinase polymerase amplification) primer group, sgRNA (single guide ribonucleic acid) and a probe, the RPA primer group is composed of an upstream primer as shown in a sequence 1 in a sequence table and a downstream primer as shown in a sequence 2 in the sequence table; the sequence of the sgRNA is shown as a sequence 3 in the sequence table. The detection method disclosed by the invention has the characteristics of rapidness, accuracy, strong specificity, high sensitivity, easiness in distinguishing a detection result, cost saving and the like, provides technical support for early diagnosis and monitoring of the strawberry vein banding virus, and has very important significance on prevention and control of field virus diseases.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention provides a fusion strain RH-0203 and application of the fusion strain RH-0203 to prevention and treatment of tomato brown wrinkled fruit virus diseases, and belongs to the technical field of microorganisms. The fusion strain is named as RH-0203 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 33388. The fusion strain is used for preparing a microbial agent, and the microbial agent is obtained by inoculating the fusion strain RH-0203 into a photosynthetic bacterium liquid culture medium and culturing at 30 DEG C under anaerobic illumination conditions. By means of microbial control, the tomato brown wrinkled fruit virus disease is effectively controlled, chlorophyll synthesis is promoted and defensive enzyme activity is enhanced while crop disease resistance is improved, and good application prospects and popularization value are achieved.