158 results about "Virus diseases" patented technology

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention provides a compound disinfectant for preventing and treating aquaculture virus diseases and a preparation method thereof. The composite disinfectant is prepared by introducing polyhexamethylene guanidine or a salt thereof, a quaternary ammonium salt compound, metal nanoparticles and an auxiliary agent into a water system as main raw materials, and is prepared by the following steps: adding a quaternary ammonium salt compound solution and a penetrating agent into a polyhexamethylene guanidine or salt solution thereof, and uniformly stirring and mixing to obtain a mixed solution A; slowly dropwise adding the metal nanoparticle dispersion liquid into the mixed solution A, starting ultrasonic treatment at the same time, and uniformly stirring and mixing to obtain a mixed solution B; dropwise adding the pH regulator solution into the mixed solution B, regulating the pH, adding the preservative solution, supplementing water, uniformly mixing, standing and curing to obtain the composite disinfectant. The compound disinfectant aims at achieving synergistic interaction, finally achieves the comprehensive prevention and treatment effects of being efficient, low in toxicity, broad in spectrum and not prone to inducing drug resistance, and particularly achieves efficient and safe prevention and control on fish, shrimp and crab virus diseases so as to meet the urgent requirement for healthy aquaculture.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention relates to the technical field of image processing, and discloses an improved StarNet-YOLOv13-based unmanned aerial vehicle field tobacco virus disease lightweight detection method, which comprises the following steps: acquiring a to-be-detected image of a field tobacco plant, and then inputting the to-be-detected image into a trained tobacco virus detection model to obtain a detection result of the tobacco plant; wherein the tobacco virus detection model is obtained by replacing a backbone network with a star network, replacing a C3K2 module in a neck network with a feature fusion module and replacing a detection head with a DetectMBConv detection head in a YOLOv13 model; the feature fusion module is constructed by introducing a partial convolution mechanism into the C3K2 module; thus, through the star network (StarNet), the feature fusion module (DSC3k2PConv module) and the DetectMBConv detection head, the small target scab detection capability and the complex scene robustness can be improved while the parameter and calculation overhead can be reduced, so that the tobacco virus detection model can meet the deployment requirement of the computing power limited equipment side, such as an unmanned aerial vehicle, and the detection precision is ensured at the same time.

The invention discloses application of TBR2 protein as well as a coding gene and a molecular marker thereof in resisting tomato brown wrinkled fruit virus diseases, and belongs to the technical field of gene engineering. According to the invention, a novel anti-disease gene TBR2 for resisting the tomato brown wrinkled fruit virus disease is identified on a chromosome 4 of tomatoes; the disease-resistant gene has stable disease resistance, and the resistance of the variety to the tomato brown crinkled fruit virus disease can be obviously improved by backcrossing and transbreeding the disease-resistant gene into a sensitive cultivar. Based on the anti-disease gene TBR2, the invention further develops molecular markers related to the tomato brown wrinkled fruit virus disease resistance, and the molecular markers can be used for identifying plant materials resistant to the tomato brown wrinkled fruit virus disease and cultivating plant varieties resistant to the tomato brown wrinkled fruit virus disease. The method is of great significance in accelerating the breeding process of disease-resistant tomatoes.

The invention discloses an anti-disease gene, an anti-disease protein, a molecular marker and application of tomato brown crinkled fruit virus disease, and belongs to the technical field of gene engineering. According to the invention, through a map-based cloning method, a new anti-disease gene TBR3 of the tomato brown fruit wrinkling virus disease is identified in a tomato chromosome 2 for the first time. The TBR3 gene is over-expressed in a plant body, so that the disease resistance of the plant to the tomato brown crinkled fruit virus disease is remarkably improved. Aiming at the disease-resistant gene TBR3, the invention also designs a molecular marker related to tomato brown crinkled fruit virus resistance, and tomato plants carrying a disease-resistant genotype and a susceptible genotype can be accurately distinguished, so that the molecular marker can be widely applied to early disease-resistant breeding and screening, and the problems of difficult early judgment and low breeding efficiency in a breeding process are solved.

The invention discloses insect lysozyme capable of preventing and treating rice virus diseases and application of the insect lysozyme. According to the invention, three pieces of lysozyme (RdLYZI1, RdLYZC1 and RdLYZC2) with remarkable inhibitory activity on various rice viruses are innovatively excavated from the body of a natural transmission vector insect of the viruses, and the amino acid sequences of the lysozyme are shown as SEQ ID NO.13, SEQ ID NO.14 and SEQ ID NO.15. The invention further discloses a preparation method of the lysozyme. Experimental verification shows that the lysozyme RdLYZI1, the lysozyme RdLYZC1 and the lysozyme RdLYZC2 can effectively inhibit replication and accumulation of various rice viruses in plants and vector insects, have a remarkable prevention and treatment effect on related virus diseases and relieve plant growth obstacles caused by the viruses. The invention provides a novel biological prevention and control strategy which is environment-friendly and high in targeting property for green prevention and control of rice virus diseases, and has an important application prospect.

Herpesviridae glycoprotein B (gB) polypeptides comprising a modified ectodomain is stabilized in the prefusion state, enabling development of inhibitors and vaccines directed against these viral pathogens. Modifications to DI, DII, and DV subdomains are important to stabilization of gB in the prefusion state, and additional modifications result in a further improved stabilization of gB in the prefusion state. The gB can be derived from an Epstein Barr Virus (EBV), a Human Cytomegalovirus (CMV), a Human Herpesvirus 6 (HHV6), a Herpes Simplex Virus 1 (HSV1), or a Varicella Zoster Virus (VZV). Polypeptides, nucleic acid constructs, compositions, and methods of using same to elicit an immune response are described.

The invention relates to application of RING type rice E3 ubiquitin ligase OsCOP1 in prevention and control of rice virus diseases, and belongs to the field of plant genetic engineering technology and disease prevention and control.

The invention discloses a recombinant salmonella choleraesuis nuclease regulation vector as well as a construction method and application thereof. After oral administration, the recombinant salmonella choleraesuis nuclease regulatory vector rSC0140 enters host immune cells, c-di-AMP is released, a host STING pathway is activated, and a broad-spectrum antiviral effect can be achieved; the recombinant strain rSC0140 is proved to be capable of activating STING pathways in macrophages and mice, causing antiviral states of the macrophages and the mice and resisting challenge of various influenza viruses; the recombinant strain has broad-spectrum antiviral efficacy and is suitable for preventing and treating various virus diseases clinically.

Vaccine compositions for protecting a subject against diseases caused by viral pathogens of the Picornaviridae family are disclosed. These vaccines comprise nucleic acids encoding the capsid polyprotein (P1 polyprotein) mixed with the viral 3C protease that processes the P1 polyprotein, and encapsulated within nanoparticle carriers. Methods of preventing, reducing, inhibiting, or delaying the symptoms of an infection caused by a viral pathogen, or of inducing an immune response against a viral pathogen in a subject are provided. Methods of making the vaccines are also described.

This invention discloses a method for inhibiting Tamdy virus infection and the use of TCP1 inhibitors. Specifically, this invention discloses a method for inhibiting Tamdy virus (TAMV) infection of host cells, the method comprising the step of reducing or inhibiting the expression level and / or activity of the TCP1 gene or its encoded protein in host cells, wherein the reduction or inhibition of the expression level and / or activity of the TCP1 gene or its encoded protein is achieved by methods selected from the group consisting of: gene mutation, gene knockout, gene interruption, RNA interference technology, gene editing technology, introduction of inhibitors of genes or proteins, or combinations thereof.

The invention discloses primers, a kit and a method for detecting four viruses in lentinus edodes. According to the invention, LAMP (loop-mediated isothermal amplification) primer groups are respectively designed and screened according to four viruses in the lentinula edodes, and the four viruses are respectively a Lentinula edodes dominant virus 1 virus, a Lentinula edodes dominant virus HKB virus, a Lentinula edodes dominant gene-like virus 1-Hunong-1 virus and a Lentinula edodes dominant virus 5 virus. The detection system is micro-quantized, the local concentration of the target sequence is improved from the side, the detection limit is greatly improved, and the lower detection limit can reach 10 copy / microliter.

The present invention relates to a pharmaceutical composition for preventing or treating coronavirus disease 2019, and more specifically to a pharmaceutical composition for preventing or treating coronavirus disease 2019 found by drug repositioning technology using drug virtual screening technology. The pharmaceutical composition for preventing or treating coronavirus disease 2019 according to the present invention is a composition obtained by finding new uses for drugs, which have already been proven effective, for preventing or treating coronavirus disease 2019 by drug repositioning technology. The pharmaceutical composition is useful because it has significantly lower side effects than new drugs and can be rapidly applied to clinical practice.

The invention relates to the technical field of aquatic product culture, and particularly discloses application of zinc in preparation of a product for preventing and treating siniperca chuatsi rhabdovirus infection. By adding a proper amount of zinc ions into a mandarin fish cell culture system or culture environment, the application of zinc after infection in forms of zinc sulfate and the like can significantly inhibit protein expression and virus proliferation of the mandarin fish rhabdovirus, and has no toxic or side effect on host cells within a certain concentration range. The novel nutritional immune prevention and control strategy which is safe, economical and easy to operate provides an effective means for prevention and control of siniperca chuatsi virus diseases, and has important application prospects and economic values.

The invention discloses an isatis root compound preparation and a method for preventing and controlling tomato mosaic virus, and relates to the technical field of agricultural planting, the isatis root compound preparation is prepared from the following raw materials in parts by weight: 15-20 parts of isatis root, 15-20 parts of radix sophorae flavescentis, 10-15 parts of scutellaria baicalensis, 10-15 parts of szechwan chinaberry fruit, 8-12 parts of radix bupleuri, 8-12 parts of rheum officinale, 8-12 parts of astragalus membranaceus, 8-12 parts of radix stemonae, 5-8 parts of fructus forsythiae and 5-8 parts of saponin. The preparation method comprises the following steps: weighing the traditional Chinese medicine decoction pieces, fully mixing and crushing; adding clear water according to a mass ratio, uniformly stirring, heating and decocting; cooling, and adding a microbial agent for fermentation; according to the method, the traditional Chinese medicine compound preparation with the radix isatidis and the radix sophorae flavescentis as monarch drugs is adopted, the tomato mosaic virus is prevented and controlled in a green mode, the occurrence of virus diseases in the tomato growth period can be effectively prevented and controlled, and chemical pesticides are reduced or not used. And the traditional Chinese medicines are used for replacing chemical pesticides, so that the method is green and safe, no chemical pesticide is used in the whole tomato production process, and real zero pesticide residue is realized.

The invention provides a fusion strain RH-0203 and application of the fusion strain RH-0203 to prevention and treatment of tomato brown wrinkled fruit virus diseases, and belongs to the technical field of microorganisms. The fusion strain is named as RH-0203 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 33388. The fusion strain is used for preparing a microbial agent, and the microbial agent is obtained by inoculating the fusion strain RH-0203 into a photosynthetic bacterium liquid culture medium and culturing at 30 DEG C under anaerobic illumination conditions. By means of microbial control, the tomato brown wrinkled fruit virus disease is effectively controlled, chlorophyll synthesis is promoted and defensive enzyme activity is enhanced while crop disease resistance is improved, and good application prospects and popularization value are achieved.

This provides methods for treating coronavirus disease 2019 (COVID-19). [Solution] This disclosure provides a chimeric adenovirus vector comprising nucleic acids encoding coronavirus disease 2019 (COVID-19) protein and an adjuvant, and a method of using the vector to induce an immune response to the SARS-CoV-2 protein for the purpose of treating COVID-19.

The application discloses a short peptide PSK3 and a preparation method and application thereof, and belongs to the technical field of genetic engineering. The application discloses a short peptide PSK3 belonging to the phytosulfokine (PSK) family, and the short peptide PSK3 is prepared by a chemical synthesis method. The short peptide PSK3 is applied to tobacco seedlings, and the PVY virus content in the tobacco can be significantly reduced compared with a control group, indicating that the short peptide PSK3 can be used for improving the resistance of the tobacco to the potato Y virus disease.

The present disclosure provides recombinant adeno-associated virus (rAAV) virions with an engineered capsid protein. In particular, the disclosure provides AAV9 virions with engineered AAV9 capsid, AAV5 / 9 chimeric capsid or combinatory capsid that achieves increased transduction efficiency in cardiac cells, increased cell-type selectivity, and / or other desirable properties.

The invention relates to the technical field of plant growth regulation, in particular to application of berberine based on ACAA targets in resisting plant viruses and promoting plant growth. The invention reveals that berberine plays a role by specifically targeting acetyl coenzyme A acyltransferase and up-regulating the expression of the acetyl coenzyme A acyltransferase for the first time. The clear compound-target-pathway mechanism overcomes the defect that the existing plant source product mechanism is unknown. As the target spot is clear, the application dosage can be accurately controlled, so that the stability and predictability of field antiviral and growth promoting effects are ensured, and a foundation is laid for large-scale agricultural application. An ACAA target is activated by berberine, and an antiviral defense mechanism and an energy metabolism growth pathway of a plant can be synchronously started, so that the plant growth is effectively promoted while virus diseases are prevented and treated. The disease resistance-growth negative correlation problem existing in agricultural production for a long time is solved, and the dual requirements of modern agriculture for precise prevention and control and yield increase and quality improvement are met.

This invention discloses a resistance gene, resistance protein, molecular marker, and their applications against tomato brown fruit wrinkling virus disease, belonging to the field of genetic engineering technology. Using map-based cloning, this invention identifies for the first time a novel resistance gene against tomato brown fruit wrinkling virus disease in tomato chromosome 2. TBR3 By overexpressing in plants TBR3 Genes that significantly enhance plant resistance to tomato brown fruit wrinkling virus disease. Targeting disease resistance genes. TBR3 This invention also designed molecular markers related to resistance to tomato brown wrinkled fruit virus disease, which can accurately distinguish tomato plants carrying disease-resistant genotypes and disease-susceptible genotypes. Therefore, it can be widely used for early disease-resistant breeding screening, solving the problems of difficult early judgment and low breeding efficiency in the breeding process.

Described are optimized RNAi agents, compositions that include RNAi agents, and methods for inhibition of coronavirus (CoV) viral genome. The optimized CoV RNAi agents and RNAi agent conjugates disclosed herein inhibit the expression of a SARS-CoV-2 viral genome, and the targeted portions of the genome are conserved across a variety of known coronaviruses. Pharmaceutical compositions that include one or more optimized CoV RNAi agents, optionally with one or more additional therapeutics, are also described. Delivery of the described CoV RNAi agents to pulmonary cells, in vivo, provides for inhibition of CoV viral genome expression, including SARS-CoV-2, which can provide a therapeutic benefit to subjects, including human subjects, for the treatment of various diseases including COVID-19.

Provided herein are antibodies that bind to neuraminidase (NA) of different strains of influenza B virus, host cells for producing such antibodies, and kits comprising such antibodies. Also provided herein are compositions comprising antibodies that bind to NA of different strains of influenza B virus and methods of using such antibodies to diagnose, prevent or treat influenza virus disease.

The invention discloses preparation and application of furoic acid and a bactericidal composition thereof, and relates to the technical field of biopesticides, the furoic acid comprises furoic acid, the chemical structural formula of the furoic acid is shown in the specification, the purity is greater than or equal to 98%, and the furoic acid is prepared by the following steps: mixing furfural, a sodium hydroxide aqueous solution and copper oxide, introducing oxygen at 50-60 DEG C, stirring and reacting for 4-6 hours, and filtering to obtain a filtrate; extracting, acidifying, recrystallizing and drying to obtain furoic acid; according to the preparation and application of the furoic acid and the bactericidal composition thereof, the furoic acid with a specific structure is scientifically compounded with screened specific biopesticides (kasugamycin, boscalid and ningnanmycin), and the ratio range (for example, the ratio of furoic acid to kasugamycin is 1: 10-10: 1) with a remarkable synergistic effect is optimized and determined; according to the composition disclosed by the invention, the components are effectively complemented on the action mechanism, and the defects that the traditional single pesticide is single in action target and easy to induce drug resistance are overcome, so that the overall control effect of the composition on various plant diseases (including bacteria, fungi and virus diseases) is remarkably improved.

Disclosed herein are compositions and methods for improving pancreatitis through pharmacological manipulation of calcium signaling. Such compositions and methods can be used to improve symptoms of acute or chronic pancreatitis or to reduce the likelihood or severity of pancreatitis in individuals at risk for the condition. In other embodiments, disclosed herein are compositions and methods for improving viral disease through pharmacological manipulation of calcium signaling. In further embodiments, disclosed herein are compositions and methods for improving Thl7-induced disease through pharmacological manipulation of calcium signaling.

The invention discloses an antiviral polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1 or 2. The invention discovers that the antiviral polypeptide shows remarkable broad-spectrum inhibitory activity on DNA (deoxyribonucleic acid) virus (HSV-1), RNA (ribonucleic acid) virus (H1N1) and enveloped virus (VSV), and the inhibition rates of the antiviral polypeptide on the DNA virus (HSV-1), the RNA virus (H1N1) and the enveloped virus (VSV) respectively reach 50.54%-75.18%, 73.06%-96.30% and 64.78%-98.68% under the concentration of 10 mu M. Meanwhile, the polypeptide has excellent safety, the half toxicity concentration in each cell line is higher than 50 mu M, and the cell survival rate under the effective antiviral concentration exceeds 90%. The invention effectively overcomes the technical defects of narrow spectrum, easy generation of drug resistance and insufficient safety of the existing antiviral drug, and provides an important candidate molecule for developing a new generation of antiviral drugs.

The application discloses a hybridoma cell strain secreting monoclonal antibody PVY-5, the monoclonal antibody and application, the hybridoma cell strain 4B4D8C3 can secrete anti-potato Y virus monoclonal antibody, the preservation number of the hybridoma cell strain is CCTCC NO: C2022282, the monoclonal antibody PVY-5 has specific immunoreaction with the coat protein of potato Y virus, the heavy chain variable region amino acid sequence of the monoclonal antibody PVY-5 is as shown in SEQ ID NO.6, the light chain variable region amino acid sequence is as shown in SEQ ID NO.7, the sensitivity reaches 1:5120 times dilution by using direct ELISA method detection to the leaf of potato Y virus disease infection, the monoclonal antibody PVY-5 can also be used to prepare ELISA kit, or colloidal gold kit, or nano analog enzyme kit, and the potato Y virus disease can be detected quickly, sensitively, economically and accurately.

The present invention provides a novel variant AAV capsid protein, a recombinant adeno-associated virus (rAAV) virion comprising same, and a pharmaceutical composition for the prevention or treatment of retinal or liver disease, comprising the rAAV virion.