74 results about "Japanese encephalitis" patented technology

The invention relates to a mosquito-repelling bactericide. The raw material formula comprises main ingredients, wherein the main ingredients comprise the following components: clove, folium artemisiae argyi, honeysuckle, elsholtzia, radix angelicae, purple perilla, calamus, mint and agastache rugosus. The mosquito-repelling bactericide disclosed by the invention has the beneficial effects that according to the common sense and principle that mosquitoes bite to suck blood and spread diseases, the bactericide has the main effect of repelling mosquitoes, and harmful pathogenic bacteria and bacteria in air can be killed by virtue of the flavor volatilized by the traditional Chinese medicines, so that the family members are prevented from being troubled by the mosquitoes and are even prevented from suffering from various diseases due to mosquito bites, and people are kept away from diseases such as malaria, filariasis, epidemic encephalitis B and dengue fever.

The invention discloses a kit for detecting a plurality of mosquito borne pathogens and a detection method thereof. The plurality of mosquito-borne infectious pathogens are detected by using multiplex polymerase chain reaction (PCR) combined with a liquid chip, and a yellow fever virus (YFV), dengue fever viruses (DV) I-IV type, epidemic encephalitis B (japanese encephalitis) virus (JEV), plasmodium (Plasmodium), (including plasmodium vivax (PV), plasmodium falciparum (PF), plasmodium malariae (PM), plasmodium ovale (PO)), a west nile virus (MNV) and a chikungunya virus (CHIKV) can be detected in parallel one time. The kit has the advantages of being large in flux, high in specificity and sensitivity, stable in result, good in repeatability, simple to operate and fast in detection speed.

The invention discloses a pharmaceutical composition for treating diseases including epidemic type B encephalitis and hepatic coma, which has the advantages of wherein the bolus has the advantages of high biological availability, quick-speed medicine release, quick-speed effect, less toxic and side effects, higher medicinal content, smaller amount of administration, accurate administration dosage, easy administration, low price, and facilitated carrying. The preparation is prepared through the conventional injection extracting processes.

The invention provides a method for industrially producing porcine Japanese encephalitis (JE) vaccines by utilizing a bioreactor. The method comprises the following steps: (1) sterilizing a microcarrier and the bioreactor, then inoculating cells for vaccine preparation for culturing, inoculating JE viruses when dense monolayers are formed on the cells on the microcarrier, and continuing culturing to reproduce the viruses; (2) stopping culturing and harvesting virus suspension when cytopathy reaches more than 80%; (3) carrying out ultrafilter concentration as well as virus inactivation on the harvested virus suspension; and (4) purifying the inactivated viruses by adopting column chromatography to prepare inactivated vaccines. In the method, the technology of using the microcarrier in the bioreactor for culture is adopted to carry out high density culture of the cells to produce the porcine JE vaccines. Compared with the traditional spinner bottle production method, the method has the following advantages: the automatic control degree is high, so production can be monitored in real time; the labour is saved, thus reducing the cost; the land for production is few, thus being easy to enlarge the scale of production; and the produced viruses have high titer, so the batch-to-batch variation is small, the product quality is stable and the side reaction is low.

The invention provides a Japanese encephalitis/dengue chimeric virus, which is obtained by replacing a prM/E gene in Japanese encephalitis cDNA clone with the prM/E gene of dengue virus type II. IFA and Western blot confirm that the chimeric virus can express E protein of a dengue virus and can stabilize passage in C6/36 cells. The invention lays a foundation for developing a dengue virus vaccine. The chimeric virus can be taken as a vaccine to immunize animals so as to enable the animals to obtain immune protection against the dengue virus.

The invention provides Japanese encephalitis (JE) vaccine composition. The JE vaccine composition comprises a JE virus antigen of immunization effective quantity and water based vaccine adjuvant. The invention further provides a preparing method and application of the JE vaccine composition. The vaccine composition has good immunogenicity and immunizing protection. The immune effect of the JE vaccine composition is remarkably superior to that of single pig JE live vaccine and other live vaccine diluted by immunologic adjuvant. The clinical application prospects are good.

The invention relates to element fabric health-improving protective clothing capable of killing and repelling mosquitoes, which belongs to the technical field of the production of healthy textile multifunctional textile fabric clothing. More than 360 types of mosquitoes are recorded in China. The mosquitoes are fond of sucking blood of warm-blooded animals, infect malaria, filariasis, yellow fever, dengue, epidemic encephalitis B and even H1N1 flu and threaten human health. The element fabric health-improving protective clothing, which is produced by performing nanometered disposal on mixed materials of a plurality of trace elements needed by a human body, a broad-spectrum powerful silver antibacterial agent, an anti-mosquito finishing agent SCJ-951, mosquito-killing pyrethrin and the like and adding the mixture into spinning solution of cotton, flax, fur, polypropylene fiber, polyamide fiber, acrylic fiber and the like, kills and repels mosquitoes and improves health.

The invention provides a method for preparing a swine epidemic encephalitis B inactivated vaccine. According to the method, a Chinese hamster lung cell adaptive virus screened by bacteriophage plaque cloning and purification is capable of generating cytopathic effect, the virulence is stabilized at 107.0-108.0TCID50/mL, and the immunogenicity is stable; the swine epidemic encephalitis B virus is multiplied by utilizing the hamster pulmonary cell system has good virus adaptability and high virus virulence of prepared vaccine. According to the method, a virus concentrating process technology which sequentially performs separating and concentrating is adopted, and virus solution and cell debris can be separated by utilizing a hollow fibrous membrane clarifying and purifying system, so that the problems that the membrane is easily blocked, is sensitive to shearing force and easily aggregated when virus particles are concentrated can be solved, side effects of an inactivated vaccine finished product can be effectively reduced, and the safety of the inactivated vaccine can be improved. Binary ethyleneimine is used as an inactivator instead of formaldehyde and has small destruction on virus antigen, so that the completeness of effective immunogen in the antigen can be guaranteed.

The invention relates to a sweet wormwood ointment with anti-mosquito and anti-malaria effects. The ointment is mainly prepared from the following raw materials: nepeta oil, menthol, dementholized peppermint oil, sweet wormwood oil, blumea oil, lemongrass oil, eugenol, artesunate, alpha-bisabolol and an ointment excipient, prevent mosquito bite well, and further prevent rapid spread of diseases which mainly take mosquitos as media such as malaria, dengue fever, filariasis or epidemic encephalitis B. The sweet wormwood ointment further has effect of rapidly relieving itching and removing swelling on itching and swelling which are caused by mosquito bite.

The invention discloses a gene chip and a kit for detecting a pig epidemic type B encephalitis virus and/or pig porcine reproductive and respiratory syndrome virus. The gene chip and the detection kit disclosed by the invention can accurately and effectively detect the pig epidemic type B encephalitis virus and the pig porcine reproductive and respiratory syndrome virus and are high in specificity, high in sensitivity, short in time, fast in detection speed and good in application prospect.

The invention discloses a gene chip and kit for detecting swine epidemic encephalitis B viruses (SEEBVs) and/or swine fever viruses (SFVs). The gene chip comprises a solid phase carrier and a probe immobilized on the solid phase carrier, wherein the probe comprises any one or two oligonucleotide fragments shown in SEQ ID NO:1 or 2 and/or any one or two oligonucleotide fragments shown in SEQ ID NO:3 or 4. The kit comprises the gene chip and reagents for amplifying genes of the SEEBVs and/or the SFVs. The gene chip and the detection kit can be used for accurately and effectively detecting the SEEBVs and the SFVs, have strong specificity, high sensitivity and good application prospects, consume short time and are fast in detection.

The invention relates to an oral preparation of a drug combination of heartleaf houttuynia and honeysuckle, including the volatile component, the water soluble component and the spirit soluble component of the heartleaf houttuynia and the honeysuckle. The drug of the invention is mainly used for the symptoms of the upper respiratory infection, the bronchial pneumonia, the acute epidemic type-B encephalitis, the viral infection, the gynecological inflammation, the fever after the operation etc. The invention also relates to the extraction method of the main components which contain the drug combination and the preparation method of the drug preparation. The drug nature test indicates that compared with the market injection preparation, the drug of the invention has the advantages of good curative effect, low side effect, convenient use, cheap price etc.

The invention relates to a preparation method of a vaccine for epidemic encephalitis B, which comprises the processes of cell culture, virus propagation and vaccine preparation. After cells overgrow with a single layer, a seed culture of viruses and a culture solution are inoculated, and the use quantity of the seed culture of the viruses is that every 100cm<2> of cells is inoculated with 1ml of seed culture of the viruses. The culture solution of the viruses mainly comprises 90-95% of MEM culture solution, 3-5% of calf serum and 7.5% of sodium bicarbonate solution the pH of which is regulated to 7.2-7.6. The vaccine is prepared by adding human serum albumin with the final concentration of 0.3% after being clarified and filtered. The invention adopts an SA14-14-2 attenuated strain to produce an encephalitis purified vaccine. Compared with a vaccine produced by a P3 strain, the attenuated strain is safer and more effective. The invention has the advantages that: (1) the attenuated strain is used for preparing the purified vaccine, which has good safety; (2) the inactivation effect of inactivating the viruses by using beta-propiolactone is good, and an inactivator is naturally degraded and has no residue; and (3) the column chromatography purification property is mild, and the injury to the viruses is small.

The invention discloses phlegm eliminating and convulsion arresting powder and application thereof. The phlegm eliminating and convulsion arresting powder is prepared from roasted scorpion, centipede, defatted croton seed powder, bezoar, borax, realgar, arisaema cum bile, unibract fritillary bulb, tabasheer and musk. The formula is reasonable, and the powder has a good treatment effect, and can effectively treat diseases such as Japanese encephalitis, pneumonia, epidemic cerebrospinal meningitis, toxic bacillary dysentery, pertussis encephalopathy and the like.

The invention relates to a recombinant E protein agent box for detecting Japanese cerebritis antibody in the field of biology technology. The agent comprises: ELISA batten which uses the recombinant E protein as the coated antibody, PBS solution, blood dilution solution, color developing base material, ending solution, JEV anodic serum and JEV cathodic serum and so on. The method can be used in other serum sample detection after peculiar, sensitive and repeating detection.

The invention discloses a primer and a probe for detecting Nipah viruses by an RAA fluorescence method as well as a detection method, wherein the primer and the probe are suitable for RAA fluorescencemethod detection, Nipah virus plasmid can be detected, cross reaction with Hendra virus plasmid, foot and mouth disease viruses, porcine parvoviruses, pseudorabies viruses, porcine circoviruses, epidemic type b encephalitis viruses, reproductive and respiratory syndrome viruses, hog cholera viruses and infectious gastroenteritis viruses can be avoided, and the specificity reaches to 100 percent;the detection method is rapid, high flux is easy to realize, the detection time and the detection cost are reduced; and according to the method for rapidly detecting Nipah virus DNA based on the RAA fluorescence method, the sensitivity is high, and 74 copies/reaction is realized under the probability that the reaction detection sensitivity is 95 percent.

The invention discloses a test strip for quickly detecting a swine Japanese encephalitis antibody and a preparation method of the test strip. The test strip consists of absorbent paper, a nitrocellulose membrane, a glass cellulose membrane, a sample pad and a support object, wherein the nitrocellulose membrane contains a detection line coated by JEV E protein and a control line coated by a goat anti-JEV antibody; and the glass cellulose membrane is combined with colloidal gold marked JEV E protein. According to the invention, by adopting a membrane chromatography indirect sandwich method, a specific antibody in Japanese encephalitis immune pig or infected pig serum can be detected. When detecting a swine Japanese encephalitis antibody, the test strip disclosed by the invention is simple, convenient and quick to operate, special instrument or professional training is not required, and the result is clear and easy to distinguish; and the test strip is easy to popularize and particularly suitable for base field test and epidemiological investigation, and plays an important auxiliary role in the diagnosis of swine Japanese encephalitis.

The invention relates to a structure and applications of antisense oligonucleotide, in particular to targeting cyclin-dependent kinase 1, a structure of antisense oligonucleotide of Enterovirus 71, Dengue virus and Japanese encephalitis resistance and applications of the antisense oligonucleotide in preparing medicines for treating Enterovirus 71, Dengue virus, Japanese encephalitis, flu virus and related diseases.

The invention discloses a traditional Chinese medicine, in particular to a traditional Chinese medicine for treating epidemic encephalitis B. The medicine comprises the following raw materials in certain weight ratio: mix-fried licorice, chrysanthemum, oriental waterplantain rhizome, amur corktree bark, turmeric root-tuber, platycladi seed, juncus effuses, fructus forsythiae, bile, vietnamese sophora root, white stiff silkworm, cynanchum glaucescens, platycodon root, Chinese magnoliavine fruit, scorpion, bamboo juice, lalang grass rhizome and common bletilla pseudobulb. The medicine is simple in preparation, good in absorption effect, obvious in curative effect, free of side effects and high in safety, and is beneficial to timely alleviating symptoms of the disease.

The invention discloses a visual gene chip and kit used for detecting porcine epidemic encephalitis b viruses and/or porcine reproductive and respiratory syndrome viruses. With the visual gene chip prepared by using a specific method, detection results can be visually seen with naked eyes, and an expensive laser confocal scanner is not needed; and the visual gene chip and kit have the advantages of low cost, high sensitivity which is almost equal to sensitivity of conventional fluorescence labeling methods, good specificity, short detection time, rapid detection and good application prospects.

The invention relates to a drug used for treating Japanese encephalitis and a preparation method thereof, belonging to the field of blood products. The invention provides a drug which can effectively treat Japanese encephalitis, thus solving the technical problem. The drug for treating Japanese encephalitis of the invention is a preparation prepared by taking Japanese encephalitis human immunoglobulin as an active ingredient and adding medicinally acceptable accessories. The drug can effectively cure Japanese encephalitis and provides a novel drug choice for Japanese encephalitis patients. In addition, the Japanese encephalitis human immunoglobulin intravenous injection prepared by the method of the invention has the advantages of better quality, high yield and purification, high virus safety and great application prospect.

The invention discloses a visual gene chip and kit used for detecting porcine epidemic encephalitis b viruses and/or hog cholera viruses. With the visual gene chip prepared by using a specific method, detection results can be visually seen with naked eyes, and an expensive laser confocal scanner is not needed; and the visual gene chip and kit have the advantages of low cost, high sensitivity which is almost equal to sensitivity of conventional fluorescence labeling methods, good specificity, short detection time, rapid detection and good application prospects.