Recombinant E protein ELISA kit for detecting antibody against Japanese encephalitis in pig

A kit and protein technology, applied in the field of recombinant E protein ELISA kits, to achieve good application prospects, easy coating concentration, and easy purification methods

Inactive Publication Date: 2006-05-03
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there is no technology to establish an ELISA kit for detecting porcine Japanese encephalitis and its detection method using the expression product

Method used

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Experimental program
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Embodiment

[0062] 1. Recombinant E protein ELISA kit for detecting porcine Japanese encephalitis antibody, including the following components:

[0063] 1) ELISA strips: Purified recombinant E protein is used as the coated antigen, and each kit contains 2 strips sealed with 1% (M / V) BSA (bovine serum albumin), sealed and frozen in packaging bags. Store dry at -20°C;

[0064] 2) Washing solution: 20 times the concentration of pH 7.4 PBS solution (containing 0.05% Tween-20);

[0065] 3) Serum diluent: 1% (M / V) BSA (bovine serum albumin) 100mL;

[0066] 4) Chromogenic substrate: H has been added 2 o 2 100 mL of OPD (o-phenylenediamine) solution;

[0067] 5) Stop solution: 2M H 2 SO 4 Solution 100mL;

[0068] 6) JEV positive serum: 5mL;

[0069] 7) JEV-negative serum: 5mL;

[0070] 8) Goat anti-pig enzyme-labeled secondary antibody: 5mL.

[0071] 2. Recombinant E protein ELISA kit is used to detect porcine Japanese encephalitis antibody, the main operation method is as follows:

[...

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Abstract

The invention relates to a recombinant E protein agent box for detecting Japanese cerebritis antibody in the field of biology technology. The agent comprises: ELISA batten which uses the recombinant E protein as the coated antibody, PBS solution, blood dilution solution, color developing base material, ending solution, JEV anodic serum and JEV cathodic serum and so on. The method can be used in other serum sample detection after peculiar, sensitive and repeating detection.

Description

a technical field [0001] The invention relates to a recombinant E protein ELISA kit for detecting porcine Japanese encephalitis antibody, belonging to the field of biotechnology. It is used for the antibody detection of porcine Japanese encephalitis antibody, so as to monitor the antibody level and the distribution and prevalence of the epidemic situation. Two background technology [0002] Japanese encephalitis (Japanese encephalitis) is an acute zoonotic infectious disease transmitted by mosquitoes that infects the central nervous system caused by Japanese encephalitis virus (JEV). It mainly causes abortion and stillbirth in pregnant sows , orchitis occurs in boars, continuous high fever in fattening pigs, and encephalitis in newborn piglets. This disease not only causes huge economic losses to the pig industry, but also exists widely in many countries in the world, seriously threatening human health. In recent years, with global warming, the prevalence of Japanese enceph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/27
Inventor 陈溥言郑其升曹瑞兵周斌杨耀武
Owner NANJING AGRICULTURAL UNIVERSITY
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