7results about How to "Guaranteed specificity" patented technology

The application discloses a circRNA detection method based on Argonaute protein and rolling circle amplification. The method comprises the following steps: mixing a sample containing target circRNA to be detected with a specific reverse transcription primer and reverse transcriptase to obtain long-chain cDNA; mixing the obtained long-chain cDNA with pfAgo, gDNA and a DNA nucleic acid probe to carry out a reaction, and calculating the content of the target circRNA according to the fluorescence signal of the reaction mixture; the nucleotide sequence of the gDNA is specifically complementary to the cDNA sequence obtained by reverse transcription of the circRNA; the sequence of the DNA nucleic acid probe is specifically complementary to the sequence of the cDNA after one cutting of the cDNA by the pfAgo; and the DNA nucleic acid probe is a single-stranded DNA probe with a fluorescent label. The application can realize high-sensitivity, high-specificity and multiple target simultaneous analysis and detection of potential diagnostic biomarkers of liver cancer such as hsa_circ_0001445 and hsa_circ_0001141.

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for mycoplasma detection, a kit and a use method of the kit. The detection kit comprises a PCR (Polymerase Chain Reaction) specific primer probe, a buffer solution, Taq enzyme and an internal quality control (IC), wherein the primer probe comprises forward and reverse amplification primers aiming at mycoplasma and an internal standard and a detection probe; the sequences of the primers are shown as SEQ ID NO.1-SEQ ID NO.7 respectively. The kit disclosed by the invention can be used for detecting mycoplasmas and accurately identifying whether various biological samples such as cell banks, production raw materials, virus seeds, cell cultures and the like are polluted by the mycoplasmas, and has high sensitivity, specificity and detection accuracy. Meanwhile, by adding internal quality control (IC), whether the to-be-detected sample inhibits the amplification reaction or not can be judged, and a false negative result is prevented from being generated.

This invention discloses a method for identifying pet breast cancer lesions based on abnormal sample DBT images in the field of pet medical imaging technology. The method acquires and preprocesses 3D images of abnormal DBT in pet breast tissue with lesion annotations, generating a 2D section sequence and a three-branch sample set. A pet breast lesion feature encoder is obtained through lesion prior comparison self-supervised training. Human breast DBT anatomical knowledge is aligned with pet data to achieve cross-species knowledge transfer. The 2D section sequence is input into the encoder and detection head to obtain 2D lesion detection results. A 3D lesion body is generated through lesion spatial trajectory growth and aggregation, and the final lesion identification result is output. This invention achieves high-precision detection relying solely on abnormal samples, eliminating the need for normal samples, diffusion models, and knowledge distillation. It adapts to the physiological structural characteristics of pet breast tissue, improves the detection effect of small lesions and the accuracy of 3D localization, and is suitable for rapid clinical screening of pet breast cancer.

The application provides a Septin9 methylation detection composition and application thereof, and the composition comprises a methylation-dependent restriction enzyme, a capture oligonucleotide, a universal primer and a Septin9 methylation-specific primer; the capture oligonucleotide comprises a first universal sequence, a folding sequence and a binding capture sequence from 5' end to 3' end in sequence; the folding sequence is at least partially identical to a 5' end sequence of a Septin9 methylation site after the methylation-dependent restriction enzyme is cut; and the binding capture sequence is specifically bound to a fragment region where the detected Septin9 methylation site is located. The application is based on a methylation-dependent restriction enzyme and a universal primer fluorescence quantitative PCR technology, does not need bisulfite conversion, and realizes accurate and specific Septin9 methylation detection.

The invention provides a specific marker gene lhh2a. 2.2 of spermatogonium of larimichthys crocea as well as a screening method and application of the specific marker gene lhh2a. 2.2, and belongs to the field of molecular markers and the field of germline stem cell research. According to the invention, the nucleotide sequence of the lhh2a. 2.2 gene is as shown in SEQ ID NO: 1; through screening and analysis of a single cell transcription sequencing technology, and through in-situ hybridization, Vasa gene and PCNA gene co-localization, it is determined that the lhh2a. 2.2 gene can specifically identify spermatogonium in a proliferation state; a core tool and a prerequisite condition can be provided for establishment of a high-efficiency germ cell transplantation technology, purification of spermatogonial stem cells for in-vitro culture and construction of a transgenic pseudosciaena crocea strain.

The invention relates to a nucleic acid amplification and chromatographic detection kit based on ureaplasma urealyticum in a totally-enclosed card box and application of the kit, the kit is a kit based on an RPA isothermal amplification chromatography technology, a freeze-drying ball reagent, a liquid reagent and a nucleic acid detection chromatographic test strip are arranged in the kit, the amplification mode is RPA isothermal amplification, and the detection sensitivity is high. The detection mode is a chromatographic test strip, the 5'end of a probe of the ureaplasma urealyticum RPA is marked with digoxin, and the 5 'end of a downstream primer is marked with biotin. The 5'end of the RPA probe of the internal standard substance bacillus is marked with FAM, and the 5 'end of the downstream primer is marked with biotin. Two indexes of UU and reference genes can be simultaneously amplified in the same tube through an RPA isothermal amplification method, an amplification product is double-stranded DNA with molecular markers at two ends, compared with fluorescent PCR, the kit integrates nucleic acid extraction, amplification and detection, the nucleic acid extraction, amplification and detection are all carried out in a card box in a sealed mode, a sample adding step is not needed, operation is simple, pollution can be effectively prevented, and the kit is suitable for large-scale popularization and application. And automation of the whole process is realized.

The invention develops a dual-mode optical sensor based on CuO2 nanodots. The dual-mode optical sensor is mainly applied to detection and research of a Warburg effect of gastric cancer. The CuO2 nanodots can catalyze the reaction of o-phenylenediamine (OPD) and hydrogen peroxide (H2O2), so that the color of the solution is changed from colorless to yellow along with the increase of absorbance and fluorescence intensity. Based on the reaction, a dual-mode sensor is designed, and the activity of lactic acid and lactate oxidase is synchronously detected through colorimetry and fluorescence. In the process of catalyzing lactic acid to generate H2O2 by lactic acid oxidase, the generated H2O2 promotes the color of a solution to change remarkably, so that the sensitive detection of the activity of lactic acid and oxidase thereof is indirectly realized. As the Warburg effect of the gastric cancer causes rise of the lactic acid level, the sensor can effectively associate the lactic acid level with the metabolic characteristics of the gastric cancer. In addition, in combination with an RGB colorimetric analysis technology, a smart phone is used for capturing color changes and carrying out image quantitative analysis, a portable, low-cost and high-sensitivity detection system is formed, and the application blank of the CuO2 nanodots in the field is filled up.