21results about How to "Guaranteed specificity" patented technology

The invention provides a BMS upper computer system, and relates to the technical field of upper computer control, the BMS upper computer system comprises a BMS upper computer and a BMS, the BMS upper computer determines key data of the BMS according to a connection instruction, and sends a request seed message to the BMS; the BMS receives the request seed message; based on the request seed message, determining seed data according to the random factor, and sending the seed data; the BMS upper computer receives seed data returned by the BMS; based on a preset encryption algorithm, determining a first encryption seed result according to the key data and the seed data; the BMS determines a second encryption seed result based on a preset encryption algorithm according to the key data and the seed data, and verifies the first encryption seed result and the second encryption seed result; and when the first encryption seed result and the second encryption seed result are the same, connecting a BMS upper computer, and sending an identification binding success message to the BMS. According to the invention, the safety can be improved.

The invention provides a multi-adapter fine-tuning automobile question and answer method, computer equipment and a storage medium. The method comprises the following steps: establishing and pre-training a base large model; performing fine tuning on the base large model based on the maintenance fault problem data set to obtain a maintenance fault adapter; performing fine adjustment on the base large model based on the vehicle use problem data set to obtain a vehicle use problem adapter; performing fine tuning on the base large model based on the general field problem data set to obtain a general field problem adapter; and performing fine adjustment on the base large model based on the intention recognition task data set to obtain an intention recognition adapter. According to the invention, fine adjustment can be carried out according to problems in different fields, and the specificity of each field is ensured, so that the precision of field adaptation is improved, and consistent performance is ensured. The method is an important improvement on an existing single fine adjustment method, and the problem of field deviation can be effectively solved.

The invention discloses a head and neck squamous cell carcinoma organ as well as a culture method, a culture medium and application thereof. The culture medium is prepared from the following components based on Advanced DMEM / F12: 1 percent of Antibiotic-Antibiotic, 1 percent of GlutaMAX, 1 percent of HEPES buffer solution, 50ng / ml of FGF-10, 50ng / ml of EGF, 1nM of bFGF, 500ng / ml of Wnt3a recombinant protein, 500ng / ml of Noggin recombinant protein, 0.1 mu g / ml of R-spondin-1 recombinant protein, 1mM of N-acetylcysteine, 10mM / ml of nicotinamide and 1x of B27 supplement. The culture medium and the culture method disclosed by the invention are particularly suitable for culturing head and neck squamous cell carcinoma organoid, and the obtained organoid has important significance for researching tumor generation, a unique invasive growth mechanism, prognosis, therapeutic targets, drug screening and the like, and has wide application prospects.

The invention belongs to the technical field of glass processing, and relates to an acid-resistant protective film liquid for ultrathin glass edge processing, and a preparation method and application thereof. The acid-resistant protective film liquid comprises the following components in parts by mass: 100 parts of phenolic resin with different molecular weights; 25 to 35 parts of acrylic resin; 5.1-10 parts of an additive; the additive comprises a flatting agent and a tackifier; and 520-560 parts of a solvent. The preparation method comprises the following steps: mixing phenolic resins with different molecular weights, mixing with a solvent, and heating and stirring until the phenolic resins are completely dissolved to obtain a phenolic resin solution; mixing acrylic resin with a solvent, heating and stirring until the acrylic resin is completely dissolved to obtain an acrylic resin solution; mixing the phenolic resin solution and the acrylic resin solution to obtain a mixed resin solution; and mixing the additive with the mixed resin solution, and uniformly stirring to obtain the acid-resistant protective film solution. The problems of film thickness uniformity and poor base material adhesion of the acid-resistant protective film liquid are effectively solved, and the stability of the acid-resistant protective film liquid is improved.

The application discloses a circRNA detection method based on Argonaute protein and rolling circle amplification. The method comprises the following steps: mixing a sample containing target circRNA to be detected with a specific reverse transcription primer and reverse transcriptase to obtain long-chain cDNA; mixing the obtained long-chain cDNA with pfAgo, gDNA and a DNA nucleic acid probe to carry out a reaction, and calculating the content of the target circRNA according to the fluorescence signal of the reaction mixture; the nucleotide sequence of the gDNA is specifically complementary to the cDNA sequence obtained by reverse transcription of the circRNA; the sequence of the DNA nucleic acid probe is specifically complementary to the sequence of the cDNA after one cutting of the cDNA by the pfAgo; and the DNA nucleic acid probe is a single-stranded DNA probe with a fluorescent label. The application can realize high-sensitivity, high-specificity and multiple target simultaneous analysis and detection of potential diagnostic biomarkers of liver cancer such as hsa_circ_0001445 and hsa_circ_0001141.

The invention discloses a nucleic acid detection kit for typhoid fever, paratyphoid fever, cholera, shigella and dysentery. The nucleic acid detection kit comprises a multiple fluorescent PCR detection system, and the multiple fluorescent PCR detection system comprises a typhoid fever salmonella primer probe, a paratyphoid fever salmonella primer probe, a vibrio cholera primer probe, a dysentery amoeba primer probe and a shigella primer probe; according to the kit, specific and conservative characteristic genes of different bacteria species are selected for primer probe design, so that the specificity, the accuracy and the sensitivity of detection are ensured.

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for mycoplasma detection, a kit and a use method of the kit. The detection kit comprises a PCR (Polymerase Chain Reaction) specific primer probe, a buffer solution, Taq enzyme and an internal quality control (IC), wherein the primer probe comprises forward and reverse amplification primers aiming at mycoplasma and an internal standard and a detection probe; the sequences of the primers are shown as SEQ ID NO.1-SEQ ID NO.7 respectively. The kit disclosed by the invention can be used for detecting mycoplasmas and accurately identifying whether various biological samples such as cell banks, production raw materials, virus seeds, cell cultures and the like are polluted by the mycoplasmas, and has high sensitivity, specificity and detection accuracy. Meanwhile, by adding internal quality control (IC), whether the to-be-detected sample inhibits the amplification reaction or not can be judged, and a false negative result is prevented from being generated.

The present invention provides a cross-linked spore phenol adsorbent based on a nucleic acid aptamer functionalized magnetic hydrophobic polymer and its application, which is obtained by coupling a nucleic acid aptamer with a magnetic hydrophobic polymer, wherein the magnetic hydrophobic polymer is a copolymer of magnetic nanoparticles, methyl methacrylate and vinyl ethylene carbonate, wherein the nucleic acid aptamer can specifically identify AOH, and the magnetic hydrophobic polymer is designed according to the molecular structure of AOH, and can increase the hydrophobic interaction with AOH, and under the synergistic effect of the nucleic acid aptamer and the magnetic hydrophobic polymer, the efficient and highly specific adsorption of AOH is achieved. The cross-linked spore phenol adsorbent based on a nucleic acid aptamer functionalized magnetic hydrophobic polymer provided by the present invention can be used for the efficient and highly specific adsorption of toxin target molecules having a hydrophobic class under the premise of changing the corresponding aptamer to which it is bonded.

This invention discloses a method for identifying pet breast cancer lesions based on abnormal sample DBT images in the field of pet medical imaging technology. The method acquires and preprocesses 3D images of abnormal DBT in pet breast tissue with lesion annotations, generating a 2D section sequence and a three-branch sample set. A pet breast lesion feature encoder is obtained through lesion prior comparison self-supervised training. Human breast DBT anatomical knowledge is aligned with pet data to achieve cross-species knowledge transfer. The 2D section sequence is input into the encoder and detection head to obtain 2D lesion detection results. A 3D lesion body is generated through lesion spatial trajectory growth and aggregation, and the final lesion identification result is output. This invention achieves high-precision detection relying solely on abnormal samples, eliminating the need for normal samples, diffusion models, and knowledge distillation. It adapts to the physiological structural characteristics of pet breast tissue, improves the detection effect of small lesions and the accuracy of 3D localization, and is suitable for rapid clinical screening of pet breast cancer.

The application provides a Septin9 methylation detection composition and application thereof, and the composition comprises a methylation-dependent restriction enzyme, a capture oligonucleotide, a universal primer and a Septin9 methylation-specific primer; the capture oligonucleotide comprises a first universal sequence, a folding sequence and a binding capture sequence from 5' end to 3' end in sequence; the folding sequence is at least partially identical to a 5' end sequence of a Septin9 methylation site after the methylation-dependent restriction enzyme is cut; and the binding capture sequence is specifically bound to a fragment region where the detected Septin9 methylation site is located. The application is based on a methylation-dependent restriction enzyme and a universal primer fluorescence quantitative PCR technology, does not need bisulfite conversion, and realizes accurate and specific Septin9 methylation detection.

The invention belongs to the technical field of biology, and discloses a monoclonal antibody specifically combined with clostridium perfringens (C.p) beta 2 toxin recombinant protein, a hybridoma cell strain and application. The invention provides a monoclonal antibody which is specifically combined with beta 2 toxin protein and a hybridoma cell strain CPB2-2A4 which secretes the monoclonal antibody and has the preservation number of CCTCC NO: C2024312, the monoclonal antibody only reacts with the C.p beta 2 toxin protein and does not have cross reaction with other pathogens which infect cattle and sheep, and the specificity is extremely high; according to the kit, a competitive ELISA technology is utilized, a mouse monoclonal antibody specifically combined with C.p beta2 toxin recombinant protein is taken as a competitive antibody, C.p beta2 toxin antibodies in serum of various animal sources can be detected, and species limitation of sample sources is avoided. In addition, the kit is good in repeatability. The kit has low technical requirements during operation, can be widely popularized and used in production, and is applied to C.p epidemiological investigation and vaccine immune level monitoring.

The invention discloses a circRNA (Ribonucleic Acid) detection method based on an Argonaute protein and rolling circle amplification. The method comprises the following steps: mixing a to-be-detected sample containing target circRNA with a specific reverse transcription primer and reverse transcriptase, and reacting to obtain long-chain cDNA (complementary deoxyribonucleic acid); mixing the obtained long-chain cDNA with pfAgo, gDNA and a DNA nucleic acid probe for reaction, and calculating the content of the target circRNA according to a fluorescence signal of a reaction mixed solution; the nucleotide sequence of the gDNA is specifically complementary to the cDNA sequence obtained by circRNA reverse transcription, the sequence of the DNA nucleic acid probe is specifically complementary to the sequence of the cDNA subjected to primary cutting by pfAgo, and the DNA nucleic acid probe is a single-stranded DNA probe with a fluorescent label. According to the invention, high-sensitivity, high-specificity and multi-target simultaneous analysis and detection of potential liver cancer diagnosis biomarkers such as hsacirc0001445, hsacirc0001141 and the like can be realized.

The present invention discloses a blood hepatitis B virus (HBV) DNA and RNA dual-channel real-time fluorescence quantitative PCR detection system, and a method for simultaneously detecting HBV DNA and RNA using the detection system. The detection system includes oligonucleotide primers, reverse anchor primers and probes for amplifying DNA and RNA. The detection system and method of the present invention can be used to prepare HBV diagnostic reagents or kits. The present invention can simultaneously detect HBV DNA and RNA in one detection, and the method is simple and fast.

The invention provides a specific marker gene lhh2a. 2.2 of spermatogonium of larimichthys crocea as well as a screening method and application of the specific marker gene lhh2a. 2.2, and belongs to the field of molecular markers and the field of germline stem cell research. According to the invention, the nucleotide sequence of the lhh2a. 2.2 gene is as shown in SEQ ID NO: 1; through screening and analysis of a single cell transcription sequencing technology, and through in-situ hybridization, Vasa gene and PCNA gene co-localization, it is determined that the lhh2a. 2.2 gene can specifically identify spermatogonium in a proliferation state; a core tool and a prerequisite condition can be provided for establishment of a high-efficiency germ cell transplantation technology, purification of spermatogonial stem cells for in-vitro culture and construction of a transgenic pseudosciaena crocea strain.

The invention relates to a nucleic acid amplification and chromatographic detection kit based on ureaplasma urealyticum in a totally-enclosed card box and application of the kit, the kit is a kit based on an RPA isothermal amplification chromatography technology, a freeze-drying ball reagent, a liquid reagent and a nucleic acid detection chromatographic test strip are arranged in the kit, the amplification mode is RPA isothermal amplification, and the detection sensitivity is high. The detection mode is a chromatographic test strip, the 5'end of a probe of the ureaplasma urealyticum RPA is marked with digoxin, and the 5 'end of a downstream primer is marked with biotin. The 5'end of the RPA probe of the internal standard substance bacillus is marked with FAM, and the 5 'end of the downstream primer is marked with biotin. Two indexes of UU and reference genes can be simultaneously amplified in the same tube through an RPA isothermal amplification method, an amplification product is double-stranded DNA with molecular markers at two ends, compared with fluorescent PCR, the kit integrates nucleic acid extraction, amplification and detection, the nucleic acid extraction, amplification and detection are all carried out in a card box in a sealed mode, a sample adding step is not needed, operation is simple, pollution can be effectively prevented, and the kit is suitable for large-scale popularization and application. And automation of the whole process is realized.

The present invention belongs to the field of nano-biosensing, and specifically relates to a DNA tetrahedron combination and its application in preparing a fluorescent probe for circular RNA imaging. The DNA tetrahedron combination consists of TDN1 and TDN2, wherein: the TDN1 is formed by base complementary pairing of the DNA single strands shown in SEQ ID NO.1‑7; the TDN2 is formed by base complementary pairing of the DNA single strands shown in SEQ ID NO.1‑4 and SEQ ID NO.8, the 3′ end of the SEQ ID NO.6 is modified with a fluorescent quenching group, and the 53rd base at the 5′ end of the SEQ ID NO.5 is modified with a fluorescent reporter group. The DNA tetrahedron combination of the present invention uses entropy increase to drive the catalytic cycle reaction to accumulate fluorescent signals, and as a fluorescent probe, it has the advantages of high sensitivity, high specificity and high stability in circular RNA imaging.

The invention discloses a method for detecting aflatoxin B1 by using an on-site rapid test strip. According to the invention, a CRISPR / Cas12a system is combined with commercial early pregnancy test paper, the aflatoxin B1 extracted from food is specifically recognized by adopting a nucleic acid lock structure, a nucleic acid sequence in the lock structure is released to be combined with crRNA, the activity of CRISPR / Cas12a is started, and the concentration information of the aflatoxin B1 is converted into color information on the early pregnancy test paper by virtue of an hCG probe. And pictures are collected by photographing through the smart phone, and picture information is processed by software, so that the quantitative detection of the aflatoxin B1 in the food can be completed. According to the detection method, the detection cost and operation steps are greatly reduced, the sensitivity is high, the specificity is good, the lowest detection limit can reach 2.6 pg / mL, and the national detection standard is reached.

The present application provides a method, device, equipment and storage medium for generating a model verification sample, which relates to the field of artificial intelligence technology and can be applied to various scenarios such as cloud technology, artificial intelligence, smart transportation, and assisted driving. The method includes: obtaining a target model, and a sample training set and a proxy model corresponding to the target model; performing a non-directional attack on the target model based on the original training sample and the corresponding initial adversarial perturbation to obtain a first adversarial sample, and the first output result of the target model for the original training sample and the second output result for the first adversarial sample meet the first adversarial condition; taking the sample label of the original training sample as the expected output, performing a directional attack on the proxy model based on the first adversarial sample to obtain a second adversarial sample; performing an adversarial verification test on the second adversarial sample, and determining the second adversarial sample as a model verification sample when the adversarial verification result is a verification pass. The present application can effectively reduce the sample generation cost.

The invention relates to the technical field of organoid culture, in particular to a culture medium and a method for culturing head and neck squamous cell carcinoma organoids through the culture medium. The culture medium provided by the invention comprises 1 * B27, 150 to 500 ng / mL of R-spondin1, 50 to 200 ng / mL of Noggin, 1 to 3 mM of N-acetylcysteine, 5 to 20 mM of Nicotinamide, 30 to 100 ng / mL of EGF (Epidermal Growth Factor), 0.1 to 1 [mu] M of A83-01, 10 ng / mL of FGF10 (Fibroblast Growth Factor), 5 ng / mL of FGF2 (Fibroblast Growth Factor 2), 0.2 to 0.5 [mu] M of MCHIR99021 and 10 [mu] M of Y27632. And a DMEM / F12 culture medium is used as a basic culture medium. The culture medium provided by the invention can be used for establishing an in-vitro model of a head and neck squamous cell carcinoma patient, storing biological information and researching a tumor recurrence and metastasis mechanism. In the aspect of individual treatment of a patient, the head and neck squamous cell carcinoma organ can provide an efficient and effective model fitting the actual situation, and can help the patient to screen new drugs and comprehensively evaluate the most effective treatment mode; the successful establishment of the in-vitro model brings a new comprehensive treatment hope for clinical medication guidance of a patient.

The invention belongs to the technical field of molecular biology, and particularly relates to an RT-PCR (reverse transcription-polymerase chain reaction) detection kit for various aspergillus and candida and a use method of the RT-PCR detection kit, specifically, the detection kit comprises an RT-PCR primer probe, a buffer solution and Taq enzyme, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at aspergillus fumigatus, aspergillus niger, aspergillus flavus, aspergillus terreus, aspergillus nidulans, candida auricula and candida albicans; the sequences of the primers are respectively shown as SEQ ID NO.1-SEQ ID NO.17. The invention also discloses a method for The kit disclosed by the invention can be used for simultaneously detecting and identifying the five types of aspergillus and the two types of candida and accurately identifying pathogens, has excellent sensitivity and detection accuracy, and has a guiding effect on further treatment and infection prevention.

The invention develops a dual-mode optical sensor based on CuO2 nanodots. The dual-mode optical sensor is mainly applied to detection and research of a Warburg effect of gastric cancer. The CuO2 nanodots can catalyze the reaction of o-phenylenediamine (OPD) and hydrogen peroxide (H2O2), so that the color of the solution is changed from colorless to yellow along with the increase of absorbance and fluorescence intensity. Based on the reaction, a dual-mode sensor is designed, and the activity of lactic acid and lactate oxidase is synchronously detected through colorimetry and fluorescence. In the process of catalyzing lactic acid to generate H2O2 by lactic acid oxidase, the generated H2O2 promotes the color of a solution to change remarkably, so that the sensitive detection of the activity of lactic acid and oxidase thereof is indirectly realized. As the Warburg effect of the gastric cancer causes rise of the lactic acid level, the sensor can effectively associate the lactic acid level with the metabolic characteristics of the gastric cancer. In addition, in combination with an RGB colorimetric analysis technology, a smart phone is used for capturing color changes and carrying out image quantitative analysis, a portable, low-cost and high-sensitivity detection system is formed, and the application blank of the CuO2 nanodots in the field is filled up.