Method for simultaneously detecting multiple anti-tumor drugs in blood sample

A technology for anti-tumor drugs and blood samples, applied in the field of blood drug concentration detection, can solve problems such as indistinguishability, inability to predict curative effects, assess adverse reactions, and drug concentration deviations, and achieve reliable results and high throughput. , to ensure specific effects

Inactive Publication Date: 2020-03-27
JINAN YING SHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunological approach has certain limitations when the clinical test drug is tested together with other interferents with similar structures
Because drugs have similar chemical structures and have similar antigen surface regions, antigen-antibody reactions can occur, and it is difficult to completely distinguish them by immunological methods, resulting in deviations between the detected drug concentration and the actual situation, which cannot be better Predict efficacy and assess adverse reactions

Method used

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  • Method for simultaneously detecting multiple anti-tumor drugs in blood sample
  • Method for simultaneously detecting multiple anti-tumor drugs in blood sample
  • Method for simultaneously detecting multiple anti-tumor drugs in blood sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0076] (1) Prepare the sample to be tested

[0077] Add methanol:acetonitrile (volume ratio 1:1) solution to the serum sample and quality control sample, vortex and centrifuge, take the supernatant into a sample bottle, blow dry with nitrogen, and redissolve the sample in 70% v / v In methanol aqueous solution, filter through a 0.2 μm filter membrane to obtain the sample to be tested.

[0078] Wherein, the volume ratio of serum, methanol, and acetonitrile is 1:1:1, and the volume ratio of serum to 70% methanol aqueous solution is 1:1.

[0079] (2) Ultra-high performance liquid chromatography-mass spectrometry detection

[0080] The sample to be tested enters the chromatographic column for separation through the gradient elution mode, and the reference conditions of liquid chromatography are:

[0081] Chromatographic column: C18 column (Waters ACQUITY HPLC BEH C18 100mm×2.1mm, 1.7μm)

[0082] Column temperature: 40°C

[0083] Injection volume: 20μl

[0084] Flow rate: 250μl...

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Abstract

The invention discloses a method for simultaneously detecting multiple anti-tumor drugs in a blood sample. A pretreated sample to be detected is detected by adopting ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The pretreatment process comprises the following steps: adding serum into a mixed solution of methanol and acetonitrile, oscillating and centrifuging,taking out the centrifuged supernatant, drying, dissolving the dried powder into a methanol aqueous solution, and filtering to obtain a sample to be detected. The method can be used for simultaneously detecting 13 kinds of anti-tumor drugs such as methotrexate, 5-fluorouracil, apatinib, busulfan, carboplatin, cyclophosphamide, docetaxel, gemcitabine, imatinib, illinotecan, lenalidomide, oxaliplatin, paclitaxel and the like in blood.

Description

technical field [0001] The invention belongs to the detection field of blood drug concentration, and relates to a method for simultaneously detecting multiple antitumor drugs in blood samples. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The efficacy and toxicity of most clinical antineoplastic drugs are related to the concentration of the parent drug or its metabolites in body fluids or tissues, so monitoring the pharmacokinetic characteristics of clinical antineoplastic drugs (absorption-distribution-metabolism-elimination, ADME) , that is, absorption, distribution, metabolism and excretion, and then it becomes very important to guide clinical rational drug use. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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