530 results about "Drug concentration" patented technology

The invention discloses a method for in vitro slow release performance evaluation of a slow and controlled release preparation based on the overflow principle. A container with an upper outlet and a lower outlet is adopted to serve as a release tank, a to-be-detected medicine and a dissolution medium are placed in the release tank, a stirring device is adopted in the release tank to perform stirring, and the dissolution medium is added from the lower outlet of the release tank through a peristaltic pump; the dissolution medium dissolved with the medicine is taken away through the overflow from the upper outlet so as to offset medicine absorption or metabolism, and the slow and controlled release speed of the medicine, the total medicine overflow quantity and the total release amount are calculated by measuring the concentration of the medicine contained in the dissolution medium flowing out, the volume of the release tank and the overflow quantity. According to the method, the releasing degree of the slow and controlled release medicine can be evaluated accurately, the effective medicine release time of the slow and controlled release preparation is evaluated, and slow release performances of the slow and controlled release preparation are evaluated comprehensively.

The invention discloses an intelligent dosing system and method based on machine learning, and the method comprises the steps: S1, collecting fluidized bed operation parameters and water quality characteristic data in real time, including water flow dynamic parameters, water quality index parameters and particle morphology parameters, and constructing a time sequence characteristic database; s2, constructing a dynamic dosing model based on the fluidized bed by using data in the time sequence characteristic database, and performing joint prediction on the medicament type, the dosing amount and the operation parameters; s3, according to a prediction result output by the model, controlling the agent dosage, the agent concentration, the water flow speed and the aeration intensity; and S4, monitoring the fluidized bed and dosing control parameters in real time, and dynamically correcting the fluidized bed parameters in combination with the effluent turbidity of the fluidized bed and the particle sedimentation efficiency, so as to solve the problems that the sewage treatment effect in the prior art is poor, and medicament waste, substandard treatment and secondary environmental pollution are possibly caused.

The invention relates to the technical field of anesthesia, and discloses a personalized anesthesia scheme generation method, which comprises the following steps: S1, extracting and coding patient features; s2, modeling a pharmacokinetic model; s3, drug concentration prediction; s4, applying a PD model; s5, performing uncertainty quantification; s6, predicting and outputting; s7, updating and feeding back parameters; s8, verifying the model; in the personalized anesthesia scheme generation process, firstly, effective feature extraction and coding are carried out on multi-modal data of a patient; according to the personalized anesthesia scheme generation method, efficient feature extraction is carried out on multi-modal data (such as electronic health records, genome information and real-time monitored vital signs) through VAE and other deep learning methods, and the differences of patients in the aspects of physique, drug metabolism capability, gene background and the like can be fully described; therefore, the optimal anesthetic medication strategy is customized for each patient.

The invention provides a therapeutic drug monitoring kit and method and system, a magnetic bead extraction method is combined with LC-MS / MS detection to be applied to human blood drug concentration monitoring, a mixed magnetic bead solution containing phospholipid removal magnetic beads and to-be-detected drug adsorption magnetic beads is utilized to perform adsorption and extraction pretreatment on a to-be-detected drug in a sample, automatic treatment equipment is matched, and the concentration of the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug in the to-be-detected drug is detected. The pretreatment efficiency is high, the phospholipid content in the supernate is low, and the matrix effect and the chromatographic column washing pressure are greatly reduced. According to the therapeutic drug monitoring method provided by the invention, a unified pretreatment process is used, simultaneous extraction of multiple drugs is realized, and through a magnetic bead automatic extraction method, the pretreatment step of a sample can be rapidly completed, a therapeutic drug monitoring result can be rapidly obtained, the overall detection time is shortened, and the detection efficiency is improved. Therefore, a reliable laboratory examination basis is provided for individualized treatment.

The invention relates to the technical field of pharmaceutical preparations, and provides a minoxidil film coating agent as well as a preparation method and application thereof. The minoxidil film coating agent provided by the invention comprises 40-60% of propylene glycol, 3-7% of polyvinyl alcohol, 20-30% of ethanol, 1-7% of minoxidil and the balance of water. According to the invention, propylene glycol is taken as a solvent, a plasticizer and a transdermal absorption enhancer, polyvinyl alcohol is taken as a film-forming material, and ethanol is taken as a volatile agent, after administration, ethanol is volatilized to promote film formation of polyvinyl alcohol, and propylene glycol enhances the plasticity of the formed film, so that the effect of reducing liquid medicine loss is achieved, the local medicine concentration is increased, and the bioavailability is improved. The minoxidil liniment disclosed by the invention has excellent skin permeability, can effectively convey a medicine to a required part, and can ensure that the medicine is released at a fixed position of the skin and is accurately administered in a medicine release process, so that the stimulation of liquid medicine flow to other parts is reduced, and a good treatment effect is achieved.

The invention discloses an administration agent in-vivo release and absorption visualization method based on a CFD-PBM coupling model. The method comprises the following steps: constructing a CFD model of a physiological environment of an injection site; establishing a population balance model (PBM) of the drug particles; the PBM is embedded into a CFD model solver, multi-scale coupling simulation is carried out, and a CFD-PBM coupling model is obtained; the three-dimensional visualization engine dynamically displays drug concentration distribution and particle behaviors; reversely adjusting preparation prescription parameters by using a multi-parameter optimization algorithm; and calibrating model parameters, and generating a key data report. According to the method, the prediction precision and the research and development efficiency can be remarkably improved, and the method is suitable for development of long-acting preparations such as microspheres and implants.

The art does not provide systematic and reproducible methods to personalize dosing of multiple immunosuppressive medications after transplantation. This invention provides a method to systematize multi-drug immuno suppression management in tissue and organ transplantation using an artificial intelligence-based complex systems approach. In embodiments of this invention, immunosuppression drug dose, blood drug concentrations, donor-derived fraction of cell free DNA (dd-cfDNA %), and aspartate aminotransferase are used to indicate allograft status or a proxy for allograft status, to generate a phenotypic response surface to produce individual treatment modalities and dosages using empirically determined unique coefficients. This surface is used to calculate appropriate immunosuppression drug doses associated with the desired outcome for that patient. Embodiments of this disclosure are directed to identifying optimized combinations of inputs for the complex system of the immunosuppressed transplant patient in order to avoid transplant rejection while avoiding unnecessary toxicity and maintaining a robust enough immune response to fight infection.

The invention provides a micro-fluidic chip capable of culturing oral squamous cell carcinoma organoid and a preparation method thereof, and relates to the technical field of micro-fluidic chips, the chip comprises a four-layer structure: the first layer is a closed layer, the second layer is a culture solution / drug pool layer (containing a concentration gradient injection channel and a slow flow channel), the third layer is a matrix culture pool layer, and the fourth layer is a matrix culture pool layer. The fourth layer is a matrigel inflow layer (containing an S-shaped flow channel); the second layer and the third layer are integrally formed, and all the layers are connected through oxygen plasma welding; the problems that in the prior art, matrigel is prone to shifting, drug gradient regulation and control is poor, operation is tedious, and adaptability is insufficient are solved, the matrigel and organoids can be stably fixed, the drug concentration gradient can be precisely regulated and controlled, the nutrition supply efficiency is enhanced, the structural integration level and operation convenience are improved, and the application prospect is wide. The method is suitable for oral squamous cell carcinoma organoid culture, drug screening and tumor mechanism research.

The invention discloses an SERS (Surface Enhanced Raman Scattering) detection method for paclitaxel drug concentration in blood of a breast cancer patient, and belongs to the technical field of detection. The method comprises the following steps: performing acetonitrile two-step precipitation centrifugal treatment on a blood sample to obtain a detection sample; the method comprises the following steps: constructing a high-sensitivity SERS substrate (the volume ratio of gold nanoparticles to ethanol is 1: (90-100), and a film is formed on a water-cyclohexane interface) through self-assembly of gold nanoparticles on a liquid-liquid interface; the method comprises the following steps: dropwise adding a sample into a substrate, and detecting characteristic peaks of 1024cm <-1 > and 1053cm <-1 > by using a portable Raman spectrometer (785nm, 30mW, 0.5 s); and calculating the paclitaxel concentration in combination with a pre-established quantitative curve. The method is easy and convenient to operate and suitable for clinical bedside rapid monitoring.

The invention relates to the technical field of blood concentration detection by optical means, in particular to a clinical blood concentration detection method. The method comprises the following steps: acquiring the delay responsivity of spectral data to drug concentration and the change complexity of other wavebands except a preset characteristic waveband; further obtaining the interfered change credibility of the drug concentration; according to the morphological characteristics of the spectrum curve corresponding to each drug concentration in the preset characteristic wave band and the distribution difference characteristics of the data points between the corresponding spectrum curve and the preset spectrum curve in the preset characteristic wave band, obtaining the spectrum shape distortion of the spectrum curve corresponding to each drug concentration in the preset characteristic wave band; obtaining the processing beneficial confidence of the spectral data; filtering the spectral data to obtain filtered spectral data; and detecting the blood concentration. According to the method, interference components in spectrum detection are removed, so that the quality of spectrum data and the detection precision of the blood concentration are improved.

The embodiment of the invention provides a multi-dimensional data processing method and system for intelligent scoring of emergency patients. The method comprises the following steps: acquiring dynamic temperature data and humidity fluctuation data of a burn wound surface through a flexible wearable monitoring device; inputting the time sequence change of the dynamic temperature data into a temperature-drug release model to generate drug concentration distribution; constructing an infection diffusion map taking the anatomical structure as a topological reference, wherein the infection diffusion map comprises an edge attribute representing the flow direction of tissue fluid and a node attribute representing a necrotic region; combining the metabolic rate parameter in the node attribute and the fluid resistance parameter in the edge attribute, iteratively updating the infection propagation intensity, and generating a weight distribution diagram; matching burn depth grading standards according to geometric deformation characteristics and gradient attenuation trends of the weight distribution map, and generating infection risk dynamic scores integrated with drug concentration distribution feedback. According to the technical scheme provided by the embodiment of the invention, the multi-dimensional data processing efficiency and precision can be improved.

The application discloses a bacteriostatic and acne-removing microemulsion containing plant extracts and a preparation method thereof. The bacteriostatic and acne-removing microemulsion contains a microemulsion loaded with quercetin alone, a microemulsion loaded with sasanquasapernin alone, and a microemulsion loaded with quercetin and sasanquasapernin simultaneously. The bacteriostatic and acne-removing microemulsion containing plant extracts prepared by the application not only solves the problems of stickiness of camellia oil and poor solubility of quercetin, but also has a synergistic inhibitory effect on propionibacterium acnes, achieves the same high-efficiency bacteriostatic effect with a lower drug concentration, saves drug cost, reduces drug irritation, and expands the application of quercetin and sasanquasapernin in the cosmetic and pharmaceutical fields due to the rich pharmacological effect of the plant extracts, low side effect and safety. The microemulsion system prepared by the application is clear, transparent, uniform and stable, the preparation process is simple, the required energy is low, the cost is low, and the system is beneficial to commercial production.

The invention discloses a capsule medicament for endoscopic treatment of ulcerative colitis and a preparation method thereof, and belongs to the technical field of biological medicine, the capsule medicament is composed of a capsule matrix and a drug matrix; by adopting a dual control mechanism of an enteric layer coating and a pH response layer coating, accurate release of a medicine at a colon diseased region is ensured, medicine waste of the stomach and the small intestine is reduced, the medicine concentration at a focus is improved, mesalazine or budesonide is combined with the composite composition for treatment, inflammatory response is rapidly inhibited, acute symptoms are controlled, and the curative effect is good. A multi-mechanism synergistic treatment mode is adopted, the anti-inflammatory and repairing effects are enhanced, the clinical remission rate and the mucous membrane healing rate are remarkably improved, the pH response layer is dissolved in a colon alkaline environment, and the MMP-9 peptide cross-linking agent is subjected to enzymolysis at an inflammation part, so that the medicine is continuously released at a focus, the administration frequency is reduced, the compliance of a patient is improved, and the curative effect is good. In addition, the spherical gel particles in the medicament can prevent the medicament from being disintegrated too early in the intestinal tract, and the long-acting effect can be maintained.

The invention provides a neutron transport simulation method and device, a medium, equipment and a BNCT treatment system. The method comprises the following steps: setting a plurality of voxel grids; the voxel grids are various human tissue materials corresponding to different boron drug concentrations; performing off-line coding on each voxel grid to obtain a corresponding physical coding layer, wherein the physical coding layer is used for predicting a corresponding neutron outgoing flow and neutron flux distribution based on the neutron incoming flow of the voxel grid; acquiring a CT image of the target object, and matching the voxel grid according to the CT image and the boron medicine planned concentration to acquire a voxel model of the target object; the physical coding layers of all voxel grids in the voxel model are coupled online. According to the method, the physical coding layers of various voxel grids are generated in an off-line manner, and the corresponding neutron outgoing flow and neutron flux distribution can be quickly predicted based on the given neutron incoming flow; and coupling a plurality of voxel grids based on a patient individualized voxel model to quickly obtain neutron flux distribution in the voxel model.

The present invention belongs to the field of drug research and development, and specifically discloses a method, storage chip and device for efficiently evaluating the efficacy of in vitro combined drug therapy. The present invention directly solves the problems of complexity and high cost of experiments and data processing in the existing drug development process by adopting an AI model and a simplified experimental mode. The AI ​​model optimizes concentration selection based on historical drug data, thereby reducing redundant concentrations and unnecessary number of experiments in the experiment, and improving the sensitivity and reliability of the evaluation. By simplifying the experimental design to single drug concentration point testing and a 3x3 combined drug matrix, the complexity and cost of the experiment are significantly reduced, while the speed of data processing and analysis is accelerated. The method disclosed in the present invention avoids excessive combined drug pre-testing by directly conducting single drug and 3x3 matrix combined drug testing, which can not only more effectively identify potential synergies between drugs, but also provide more accurate and practical data to support future clinical research.

The invention provides a stem cell exosome for overexpressing A beta degrading enzyme as well as a preparation method and application of the stem cell exosome. Specifically, the invention provides an application of the stem cell exosome overexpressing A beta degrading enzyme in preparation of drugs, and the drugs are used for at least one of the following: prevention, improvement or treatment of cognitive impairment related diseases; neuronal cell damage is improved; wherein the stem cells for overexpressing the A beta degrading enzyme are prepared through lentivirus transfection. According to the stem cell exosome overexpressing the A beta degrading enzyme, the content of the A beta degrading enzyme is obviously higher than that of the exosome prepared by a traditional method. Therefore, when the compound is prepared into a medicine for treating or preventing cognitive impairment related diseases, the medicine can be efficiently conveyed to the brain, and the concentration of the medicine in the brain is remarkably improved, so that the curative effect is enhanced, the dosage of the medicine can be reduced, the side effect is reduced, and the prognosis of brain diseases is effectively improved.

The invention relates to the technical field of traditional Chinese medicine plasters, in particular to a traditional Chinese medicine ointment for preventing relapse of infantile asthma and a preparation method thereof.The traditional Chinese medicine ointment is characterized by sequentially comprising a protective backing layer, a medicine storage layer, a controlled release film layer and an application bonding layer from top to bottom; the traditional Chinese medicine plaster provided by the invention overcomes the defect that the traditional oral traditional Chinese medicine is easily influenced by a first-pass effect and individual differences through an external transdermal administration mode, can continuously maintain efficient medicine concentration at an application part and realize long-acting slow release, and the synergistic effect of the nano micro-emulsion medicine carrying system and the controlled release film layer can realize the sustained release effect of the traditional Chinese medicine. According to the traditional Chinese medicine plaster disclosed by the invention, the stable release of effective components of the medicine is ensured, the penetration rate of the medicine on a skin barrier is also remarkably improved, so that the treatment depth and the action durability of the plaster are enhanced, and meanwhile, the traditional Chinese medicine plaster aims at the pathological mechanism of infantile asthma, relieves airway inflammation, reduces airway hyperresponsiveness and mucus secretion, and has a remarkable curative effect on infantile asthma. The acute attack frequency of asthma can be obviously reduced, and the remission period is prolonged.

The invention discloses a drug targeted delivery device and method, and belongs to the technical field of intelligent medical treatment. The device comprises the following modules: an intelligent targeted recognition module for accurately recognizing a gastric cancer specific molecular marker and improving the selectivity and specificity of targeted recognition in combination with a dynamic surface modification strategy; the microenvironment real-time monitoring module is used for monitoring key physiological parameters of the gastric cancer microenvironment in real time; the intelligent path planning module is used for dynamically calculating and optimizing a drug delivery path and realizing accurate drug positioning; the personalized drug administration regulation and control module is used for accurately predicting the drug dosage demand of an individual and dynamically and finely adjusting the drug administration amount in real time; the drug resistance risk management module is used for early warning a drug resistance risk in advance and automatically generating a personalized alternative drug administration strategy through multi-dimensional drug resistance analysis and real-time early warning; and the intelligent drug release module adopts a temperature-sensitive / pH-sensitive polymer shell layer and a nano valve to control drug release, so that precise gradient release of drug concentration is realized.

The invention relates to the field of drug concentration detection, in particular to a rapid tacrolimus drug concentration detection device based on a micro-fluidic chip. The detection device comprises a blood sampling area, a separation area, a liquid medicine storage area, a waste liquid storage area and a spectrograph, the separation area is used for separating a tacrolimus medicine in a blood sample solution, and a liquid medicine sample and a waste liquid sample are obtained; the spectrograph is used for collecting liquid medicine spectral data of the liquid medicine sample in the liquid medicine storage area and waste liquid spectral data of the waste liquid sample in the waste liquid storage area, and detecting the concentration of the tacrolimus medicine in the blood sample solution according to the liquid medicine spectral data and the waste liquid spectral data. According to the invention, the accuracy of detecting the concentration of the tacrolimus drug in the blood can be improved.

The present invention provides a method and a detection device for detecting drugs and their metabolites in hair, which relates to the technical field of drug detection. The present invention collects hair samples, prepares samples with known drug concentration values, collects spectral data through a Raman spectrometer, inputs the spectral data matrix and the response variable matrix, analyzes them, generates a relationship matrix reflecting the correlation between the spectral data matrix and the response variable matrix, and further processes it to generate a weight vector reflecting the weight coefficients of the detection sample concentration at corresponding wavelengths. By analyzing the correlation between the weight vector and the spectral data matrix, a confidence vector that maximally reflects the directional relationship between the response variable matrix Y and the spectral data matrix X is generated, a back-projection matrix is generated after back-projection, and a confidence index is output. By comparing the confidence index with a confidence threshold, it is output whether the concentration detected by the Raman spectrometer in the current environment is valid.

The invention discloses a plant extract transdermal absorption dynamic regulation and control method based on artificial intelligence. The method comprises the following steps: S1, forming a structured data sequence; s2, generating preliminary prediction data; s3, forming an optimized GRU prediction model; s4, performing real-time prediction on a future transdermal absorption state by utilizing the optimized GRU prediction model, and generating prediction data of an absorption rate, a drug concentration in skin and an accumulated absorption amount; and S5, according to the prediction data and a preset drug release target, carrying out global search and optimization on drug release control parameters through the firefly population optimization algorithm, generating a corresponding dynamic regulation and control instruction, and adjusting the release rate and carrier diffusion characteristics of a drug release device. The release rhythm can be actively adjusted according to the predicted data, so that the concentration stability and the curative effect persistence of the plant active ingredients in the target cortex are ensured.

The invention relates to a flotation powdery medicament preparation mechanism and a use method, the flotation powdery medicament preparation mechanism comprises an automatic control system, a medicament preparation tank, a water inlet pipe, a stirrer, a buoy heavy hammer counterweight type liquid level meter and a medicament outlet pipe, and is characterized by further comprising a powdery medicament feeding system, the powdery agent feeding system comprises an agent box, a feeding pipe, a spiral conveyor, a discharging pipe, a metering belt, a conveying header pipe, two branch feeding pipes and an agent feeding electric control valve. The liquid level of the stirring tank is recognized through cooperation of the laser distance measuring sensor and the heavy hammer float type liquid level meter, the dosage of chemicals in the stirring tank is obtained through the calculation module, the chemicals are fed into the dispensing tank through the anti-blocking spiral ore feeder, the metering belt and the spiral conveyor to achieve quantitative conveying, and automatic water supplementing and stirring are matched. Full-process management and control of agent concentration, liquid level, dosage, recording and storage and the like are achieved, the labor cost of enterprises is reduced, the labor intensity of workers is reduced, the agent concentration is accurately controlled, and flotation quality fluctuation accidents caused by agent interruption are avoided.

The invention provides an airway atomization flow intelligent drug delivery control method and system, and relates to the technical field of medical instruments.The method comprises the steps that basic information of the age, the weight and the illness state severity of a patient is obtained, and physiological parameters of the respiratory rate and the tidal volume of the patient are collected in real time through a sensor; inputting the basic information and the physiological parameters into a fuzzy adaptive control algorithm, and initializing an individual administration rule base according to the basic information; based on the real-time change trend of the respiratory rate and the tidal volume, three initial control index values including an atomization flow value, a drug concentration value and a time interval are dynamically generated through a membership function. According to the invention, through the full-process design of data acquisition, intelligent modeling, accurate execution and data closed loop, intelligentization of atomization treatment is realized.

The invention relates to the technical field of medical instruments, in particular to an inhalation device for monitoring drug concentration in real time and a dose regulation and control method.The inhalation device comprises a bottom plate and a dry powder storage tank installed on the bottom plate, a fixing block is installed at the top end of the dry powder storage tank, and an inhalation pipe connected into the dry powder storage tank is embedded into the fixing block; a gas storage tank is installed on one side of the top end of the bottom plate, a connecting pipeline is connected between the gas storage tank and the dry powder storage tank, a gas inlet pipe connected to the rear end of the suction pipe is embedded in the top end of the gas storage tank, and a gas pump is installed on the side, close to the fixing block, of the top end of the dry powder storage tank. The air guide pipe is connected to a flow dividing pipe at the inner end of the fixing block, a plurality of injection pipes extending to the inner pipe wall of the suction pipe are embedded in the flow dividing pipe, an air bag cavity is formed in the suction pipe, and a plurality of reinforcing ribs are fixedly connected to the inner end of the air bag cavity. The pipe diameter of the suction pipe is enlarged, and concentration is reduced.

The invention provides a human bladder cancer vedicetumab drug-resistant cell strain as well as a construction method and application thereof. The cell strain is named as a human bladder cancer drug-resistant cell strain T24-RC48, and the preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024180. During construction, a method of continuously inducing at low concentration and gradually increasing the drug concentration and the treatment time of the RC48 is adopted, so that the formation of the RC48 drug-resistant cells is effectively induced, and the construction method is simple to operate and can effectively make up for the defects of the existing method. The human bladder transitional cell carcinoma RC48 drug-resistant cell strain T24-RC48 constructed by the invention can be used for researching a bladder cancer drug-resistant mechanism, promoting the development of predictive biomarkers, evaluating the antitumor activity of drugs and preparing a tumor cell model or a tumor animal model; a cell research model is provided for screening, researching and developing effective drugs and methods for overcoming bladder cancer drug resistance, and good application prospects are achieved.

The invention discloses an optical fiber sensor for drug concentration detection and a drug concentration detection system, and belongs to the technical field of sensors, the optical fiber sensor comprises a first single-mode optical fiber, a seven-core optical fiber, a second single-mode optical fiber and a multi-layer functional film; the right end of the first single-mode optical fiber, the left end of the second single-mode optical fiber and the left end and the right end of the seven-core optical fiber are respectively provided with a microsphere cavity; the microsphere cavity at the right end of the first single-mode optical fiber is welded with the microsphere cavity at the left end of the seven-core optical fiber, and the microsphere cavity at the left end of the second single-mode optical fiber is welded with the microsphere cavity at the right end of the seven-core optical fiber; the seven-core optical fiber is of a micro-conical structure with the diameter gradually increasing from the middle to the two ends. The multiple layers of functional films are stacked on the surface of the seven-core optical fiber, the lower layer is a biocompatible adsorption film, and the upper layer is an anesthetic drug specific ligand film. According to the scheme, the optical fiber sensor is simple in design structure and easy to implement, the detection limit, the sensing sensitivity and the detection stability of the optical fiber sensor are comprehensively improved, and accurate detection of the drug concentration is achieved.

The present invention discloses a nano-thermosensitive assembled gel for nasal administration, a preparation method thereof and its use, namely, mitochondrion-targeted puerarin nanoparticles, a thermosensitive gel, a preparation method thereof and its use. The nano-thermosensitive assembled gel for nasal administration of the present invention uses chitosan modified with SS-31 peptide having mitochondrion-targeting and transmembrane properties as a carrier material, and uses a thioketal cross-linking agent sensitive to reactive oxygen species (ROS) to cross-link the modified chitosan to prepare nanoparticles loaded with puerarin. Through a poloxamer thermosensitive gel system, a thermosensitive nanogel drug reservoir encapsulating the nanoparticles is constructed, and its application in the preparation of drugs for treating nervous system diseases realizes the targeting of the drug to the mitochondria of cerebral ischemic stroke injury, responsive cleavage in an environment rich in ROS, release of the drug, increase of the drug concentration in the mitochondria, and improvement of the brain bioavailability of puerarin, providing a more suitable and effective new dosage form for the treatment of stroke.

The invention belongs to the technical field of medical materials and medicine, and discloses a microneedle patch for accelerating oral mucosa wound healing and a method and application thereof, and the specific preparation method comprises the following steps: (1) preparing a substrate material; (2) preparing a photoactive initiator; (3) loading HS-38 on methacrylated gelatin; and (4) manufacturing a microneedle to obtain the HS-38 loaded microneedle patch for accelerating oral mucosa wound healing. The preparation process of the microneedle patch does not need to depend on expensive instruments and equipment, operation is easy and convenient, and the microneedle patch has the advantages of being accurate in local delivery, noninvasive and painless, controllable in local drug concentration, stable in local drug concentration and the like. The strategy not only provides an effective means for treating chronic oral wounds, but also lays a foundation for realizing clinical transformation and application of medicines, and has wide popularization and application prospects.

The invention belongs to the field of antibiotic analysis and detection, and discloses a quinolone drug SERS (Surface Enhanced Raman Scattering) detection method based on an improved silver nano-substrate and a spectral angle matching model. An improved Lee-Meisel method is adopted to synthesize silver nano sol with precisely controlled particle size, molecular adsorption is realized by utilizing electrostatic interaction of a positively charged quinolone drug and negatively charged silver nano particles, and a high-density Raman enhanced hot spot is formed after an agglomeration agent is added; the SERS spectrum is collected at the excitation wavelength of 785 nm and the integral time of 10 s, qualitative distinguishing of drugs is achieved by combining a spectrum angle matching model, and quantitative detection is achieved by constructing a standard curve through the linear relation between the characteristic peak intensity and the drug concentration logarithm. The detection limit of the four drugs is as low as 1.03-1.16 ppb, the classification accuracy rate reaches 100%, the method is obviously superior to existing similar methods, the detection matrix range is wide, such as animal food and dairy products, and universality and high sensitivity are both considered.

The invention discloses CD63 aptamer driven DNA hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel materials. In order to solve the technical problems of relatively low delivery efficiency and targeting property, short drug retention and relatively poor permeability of hydrogel in the prior art, the invention provides a CD63 aptamer driven type DNA hydrogel, the DNA hydrogel is composed of three Y-type DNA chains, L1 + L2 (SEQ ID NO.4), a CD63 aptamer (SEQ ID NO.5) and L2 (SEQ ID NO.6), and the nucleotide sequences of the three Y-type DNA chains are shown as SEQ ID NO.1-3. The DNA hydrogel provided by the invention can efficiently load the exosome, has good biocompatibility and mechanical properties, and reduces side effects caused by overlarge local drug concentration; the exosome-loaded hydrogel can be applied to exosome-loaded promotion of skin wound healing and treatment of osteoarthritis.