237results about How to "Eliminate side effects" patented technology

The invention belongs to the field of molecular biology and immunology, and particularly relates to an anti-CD3 nano antibody capable of activating the activity of T cells as well as a preparation method and application of the anti-CD3 nano antibody. The invention provides a preparation method of the anti-CD3 nano antibody. The preparation method comprises the following steps: constructing a CD3 plasmid and an overexpression cell line, and immunizing alpaca to obtain peripheral blood mononuclear cells (PBMC) of the alpaca; extracting RNA (Ribonucleic Acid) and carrying out reverse transcription to obtain cDNA; carrying out PCR amplification on the antibody gene segment and cloning the antibody gene segment to a phage display vector; and electrically transforming the vector into SS320 competent cells to obtain the nano antibody library. After two rounds of panning, detecting and screening an antibody clone capable of being combined with the CD3 protein through ELISA (Enzyme-Linked Immunosorbent Assay); an antibody sequence is selected through sequencing, and an antibody protein is expressed by using a mammalian cell high-throughput expression system; and then detecting and screening the antibody which is only specifically combined with the CD3. The method disclosed by the invention is simple, convenient and rapid to operate, and can be used for preparing active antibodies for treating potential tumors and proliferative diseases.

The invention relates to the technical field of biological medicine, and provides a targeting exosome capable of delivering nucleic acid drugs and a preparation method of the targeting exosome. The exosome comprises a fusion polypeptide, the fusion polypeptide comprises a targeting protein molecule, an exosome transmembrane protein and a nucleic acid binding domain, and the exosome can load a nucleic acid molecule through binding of the nucleic acid binding domain. According to the invention, engineering modification is carried out on the exosome, so that nucleic acid molecules can be efficiently loaded into the exosome, and meanwhile, the exosome is endowed with the capability of targeting to specific target cells, tissues and organs.

The invention relates to the field of biomedical materials, discloses a hydrogen sulfide / nitric oxide synergistic anti-inflammatory and anti-coagulation repair promoting coating as well as a preparation method and application thereof, and provides a coating with anti-inflammatory, anti-coagulation and tissue repair promoting functions through the synergistic effect of hydrogen sulfide and nitric oxide. The coating comprises a hydrogen sulfide donor which can spontaneously release hydrogen sulfide or a precursor thereof or release hydrogen sulfide under external stimulation; the coating further comprises a component capable of catalyzing the release of nitric oxide from the endogenous nitric oxide donor. The coating is covalently connected with the surface of a base material through a chemical means to form firm interface bonding; and the coating has a multi-cross-linked network structure, so that the coating is endowed with good stability, and long-term functional application of the coating on the surface of a biomedical material is ensured. The method can be widely applied to surface modification of biomedical materials, especially coating functionalization of implanted instruments such as valve clips, intravascular stents, heart valves, occluders, vascular grafts and myocardial patches.

The invention belongs to the technical field of biological medicines, and particularly relates to a pyruvate preparation for treating asthma and a preparation method of the pyruvate preparation. The pyruvate preparation is prepared from the following components in parts by weight: 10 to 12 parts of pyruvate, 10 to 12 parts of sodium chloride, 0.5 to 1 part of citric acid and 500 parts of water for injection. The pH value of the pyruvate preparation is 6 to 7. The pyruvate preparation is used for daily management of asthma patients, the purpose of inhibiting asthma is achieved by controlling increase of the number of inflammatory cells such as eosinophilic granulocytes and reducing inflammatory reaction, the effect of the pyruvate preparation is close to that of similar hormone medicine dexamethasone, and the effect of the pyruvate preparation is improved while the treatment purpose is achieved. The dependence and side effects of patients on hormone drugs are reduced.

The invention belongs to the technical field of medicines, and particularly discloses application of moss phenol glucoside in preparation of medicines for preventing and / or treating tumors. According to the method, activated CD8 + T cells, MC38-OVA cells and traditional Chinese medicine monomeric compounds are co-cultured, the traditional Chinese medicine monomeric compounds capable of effectively promoting the anti-tumor activity of the CD8 + T cells are selected by utilizing a high-content screening technology, and finally, the experiment is carried out by taking the moss phenol glucoside as a research object. In-vitro experiment results show that the moss phenol glucoside can significantly promote T cell activation, promote release of cell factors such as IFN-gamma and TNF-alpha, and enhance the killing effect of T cells on tumors. A mouse in-vivo tumor-bearing experiment result shows that the moss phenol glucoside can promote the anti-tumor activity of T cells so as to inhibit tumor growth, has no obvious toxic or side effect, can be used for preparing the medicine for preventing and / or treating tumors, and has a potential clinical application prospect and a relatively high economic value.

A method for depositing a metal compound coating on the surface of a polymer base material at low temperature comprises the following steps: placing the polymer base material in an atomic layer deposition chamber, vacuumizing, and adjusting the temperature and pressure of the chamber; activating the surface of the polymer base material by using plasma; injecting a metal organic precursor into the reaction cavity in a pulse manner at the temperature lower than 300 DEG C, and forming a uniform metal compound coating through plasma enhanced atomic layer deposition; and the redundant precursor is purged through gas, and the steps are repeated until the coating with the target thickness is obtained. According to the method, the surface of the plastic base material is modified under the condition that the temperature is lower than 300 DEG C through a plasma enhanced atomic layer deposition (PEALD) technology, the metal compound coating which is uniform in thickness and controllable in component is obtained, and the requirement for improving the biocompatibility of the surface of the implant in the medical field is met.

The invention belongs to the field of medicine, and particularly discloses a deuterated tetrahydrofuran NaV1.8 inhibitor and application thereof, and the NaV1.8 inhibitor comprises a compound shown as a formula I or pharmaceutically acceptable salt and solvate thereof; wherein R1 and R2 are respectively and independently selected from H and deuterated C1-3 alkyl groups. The NaV1.8 inhibitor adopts a deuterated tetrahydrofuran medicine, the pharmacokinetic property of the NaV1.8 inhibitor can be remarkably improved, the administration dosage and toxic and side effects can be reduced, specifically, the channel activity IC50 of the NaV1.8 is lower than 0.1 nmol / L, the inhibitory activity IC50 of the NaV1.8 is lower than 43 nmol / L, the oral pharmacokinetic parameter t1 / 2 is 5.2 + / -1.2 h, and the NaV1.8 inhibitor is remarkably superior to a commercially available VX-548 preparation. # imgabs0 #

The invention relates to the technical field of medical instruments, in particular to an electromagnetic ultrasonic device for treating osteoporosis, which comprises a bed body, an electromagnetic ultrasonic probe, a data acquisition module, a data processing module and an artificial intelligence analysis module, an ultrasonic treatment assembly and an electromagnetic treatment assembly are integrated in the electromagnetic ultrasonic probe; the ultrasonic treatment assembly emits ultrasonic waves and transmits the ultrasonic waves to bones through soft tissues for ultrasonic treatment; the electromagnetic treatment assembly generates an alternating electromagnetic field to stimulate bone cells; physiological signal data are analyzed in real time through an artificial intelligence algorithm, physiological state changes are predicted, output parameters of PEMF and LIPUS are automatically adjusted to adapt to individual physiological differences, and personalized treatment is achieved.

The invention relates to the field of hydrogel materials, in particular to a hydrogel dressing as well as a preparation method and application thereof. The deferoxamine mesylate is loaded on the gelatin microsphere material constructed by the gelatin and the genipin, so that the loading dispersion of the deferoxamine mesylate can be benefited, and the added gelatin can be in contact with matrix proteinase-9 in a diabetic foot ulcer microenvironment for degradation so as to induce the release of the deferoxamine mesylate. A first cross-linked network is formed by the 3-carboxyphenylboronic acid modified chitosan and the oxidized hyaluronic acid, and the gelatin microsphere material loaded with the deferoxamine mesylate and the epigallocatechin gallate are coated with the first cross-linked network. The hydrogel dressing realizes controllable release of the deferoxamine mesylate and the epigallocatechin gallate, so that the technical problem of uncontrollable release of the deferoxamine mesylate is solved.

The invention provides a drug combination composition for tumor chemotherapy. The drug combination composition comprises a safe and effective dose of a platinum chemotherapeutic drug and a safe and effective dose of an NAMPT inhibitor. Compared with a single platinum chemotherapeutic drug, the combined drug composition provided by the invention has higher sensitivity to cancers such as liver cancer, gastric cancer, colorectal cancer, lung cancer, cervical cancer, kidney cancer, pancreatic cancer, breast cancer and the like, the effective rate is remarkably improved, and the toxic and side effects are remarkably reduced, so that the combined drug composition has a wide application prospect.

The invention provides an antifouling developing coating material and a preparation method and application thereof.The antifouling developing coating material is of a structure shown in the formula I. The antifouling developing coating material combines antifouling and developing through a covalent binding technology, has excellent antifouling performance and developing performance, and can be applied to the field of chemical engineering. According to the invention, the stability and durability of the developing effect can be ensured, the safety is good, the application range is wider, and powerful support is provided for the safety of medical equipment and the accuracy of medical images.

The invention belongs to the technical field of biological medicine, and particularly relates to an iron ion carrier and a preparation method and application thereof. The iron ion carrier disclosed by the invention has the advantages that the iron ion carrier disclosed by the invention can have relatively good binding activity with iron ions and ferrous ions, and has relatively low cytotoxicity and relatively long in-vivo drug half-life period.

The invention discloses a microneedle patch loaded with a sorafenib and copper-doped Prussian blue nano material and application of the microneedle patch in in-situ triple-negative breast cancer treatment. The sorafenib and the copper-doped Prussian blue nano material are loaded in a needle body of the microneedle patch. The microneedle patch can accurately deliver nanoparticles and sorafenib to tumor tissues, the chemical kinetics effect is enhanced through an efficient and mild photothermal therapy, the ferroptosis of triple negative breast cancer cells is enhanced through ferroptosis induced by the nanoparticles and sorafenib in cooperation with drugs, then cell death is induced, and the curative effect is improved. And a brand-new and promising treatment method is provided for in-situ triple-negative breast cancer treatment.

The invention discloses chitosan / poly (p-dioxanone) composite hydrogel as well as a preparation method and application thereof, the hydrogel is prepared by repeatedly freezing and thawing a cross-linked polymer swelling solution formed by chitosan with the deacetylation degree of more than or equal to 95% and poly (p-dioxanone) with the viscosity average molecular weight of 1 * 10 < 4 >-1 * 10 < 6 >; wherein the use amount of the poly (p-dioxanone) is 1 wt%-18 wt% of the weight of the chitosan; according to the chitosan / poly (p-dioxanone) composite hydrogel, a poly (p-dioxanone) molecular chain with excellent flexibility and mechanical property is introduced into chitosan hydrogel with a network structure to form hydrogel with a network interpenetrating structure, so that the mechanical strength of the chitosan hydrogel is greatly improved; meanwhile, the hydrogel has good degradability and biocompatibility, and due to the good biocompatibility, the hydrogel can be applied to tissue engineering; in addition, the preparation method of the hydrogel is simple, conditions are easy to control, and the hydrogel has a wide application prospect in the field of biomedicine.

The invention relates to the technical field of prediction and evaluation of lung cancer samples, and discloses a prediction and evaluation method of a lung cancer sample, which comprises the following steps: collecting clinical information of a lung cancer patient, acquiring biological data of the lung cancer sample, sorting medical image data, extracting key genes, proteins or pathway characteristics from the biological data, important features related to the lung cancer are screened by using a statistical method or a machine learning algorithm, a proper machine learning or deep learning model is selected according to data characteristics and a prediction target, and the interpretability, stability and calculation complexity of the model are considered. According to the method, tumors with high-risk characteristics, such as tumors with high invasiveness and high metastasis potential, can be found and intervened in time through prediction and evaluation of lung cancer samples, so that the disease progress is delayed, the treatment effect is improved, the treatment effect and disease change can be monitored through regular prediction and evaluation of the lung cancer samples in the treatment process, and the treatment effect is improved. The treatment scheme is adjusted in time, and the maximization of the treatment effect is ensured.

The invention discloses Chinese wolfberry fruit composite health wine and a preparation process thereof, and belongs to the field of fermented wine preparation processes, the Chinese wolfberry fruit composite health wine is prepared from the following raw materials according to the total solid-liquid ratio: 15-25 g / L of Chinese wolfberry fruit, 6-10 g / L of cistanche, 6-10 g / L of Chinese yam, 6-10 g / L of eucommia ulmoides leaf, 4-8 g / L of dogwood pulp, 4-8 g / L of astragalus membranaceus and 1-3 g / L of liquorice; the raw materials are added in a staged material supplementing mode for liquid fermentation, specifically, fructus lycii, Chinese yam, folium cortex eucommiae, fructus corni and liquorice are added in the ingredient adjusting stage, herba cistanche and part of radix astragali are added within 6 h after yeast fermentation, and the remaining radix astragali is added after fermentation. The obtained Chinese wolfberry fruit composite health-preserving wine is obvious in fermentation fragrance, mild in medicine flavor, high in functional component content and unique in taste.

The invention is applicable to the technical field of biological medicines, and provides a frozen cell as well as a preparation method and application thereof. According to the invention, frozen human umbilical cord mesenchymal stem cells (CK-MSCs) are adopted as a carrier of mitoxantrone (MTX), so that accurate delivery and pH-sensitive controlled release of drugs at tumor sites are realized, the treatment effect is remarkably improved, and the side effects of traditional chemotherapy are greatly reduced. The freezing treatment enables the human umbilical cord mesenchymal stem cells to lose the multiplication capacity, so that the tumor formation risk is eliminated, and the treatment safety is ensured. By adjusting the immune response in the tumor microenvironment, the CK-MTX not only directly inhibits the tumor, but also enhances the anti-tumor immune defense of the body. The freezing treatment method disclosed by the invention can be applied to other types of cells, and the frozen cells can be compatible with other medicines, so that revolutionary breakthrough is brought to tumor treatment, and a new way is opened up for treatment of other diseases.

The invention provides a preparation method of a hybrid cell membrane modified nano-drug for inhibiting interment of macrophages. According to the invention, a multifunctional bionic nano-drug with a'core-shell 'structure is prepared from liver cancer cell membrane and bone marrow-derived macrophage membrane hybrid double-drug-loading carrier nanoparticles. In the application, the bionic function of macrophages is firstly realized, so that the drug is prevented from being swallowed by mononuclear macrophages in vivo, and the side effect of the drug is reduced; secondly, by utilizing the homologous targeting effect of hepatoma carcinoma cells of Hepa1-6, the aggregation of the medicine in tumors is enhanced, and the utilization efficiency of the medicine is improved; finally, the two drugs including sorafenib and Xkr8siRNA are co-delivered, so that the pharmacokinetics is simplified, and the immunotherapy effect of the liver cancer can be promoted by utilizing the inhibition of the Xkr8siRNA on the intercellular effect.

The invention provides an etomidate derivative, a preparation method thereof and application of the etomidate derivative in preparation of anesthetic drugs, and belongs to the field of chemical medicines. The structure of the etomidate derivative is shown as a formula I in the specification. Compared with etomidate, the compound disclosed by the invention has the advantage that the anesthesia time is remarkably shortened. The anesthesia time is shortened, so that some side effects after anesthesia can be relieved; rapid recovery of the autonomous regulation ability of important organs of a patient and postoperative nursing are facilitated; and the risk of wake-up delay is reduced. The etomidate derivative provided by the invention has a good application prospect in preparation of anesthetic drugs for shortening anesthesia time. And # imgabs0 #.

The invention provides a high-voltage electrolyte and a lithium ion battery, and relates to the technical field of lithium ion batteries. The high-voltage electrolyte comprises a lithium salt, a non-aqueous solvent, a first additive and a second additive, the first additive comprises a phthalic acid derivative containing a benzene ring functional group and / or a phenylboronic acid pinacol ester compound containing a cyano functional group. The electrolyte provided by the invention is beneficial to prolonging the cycle life of the battery under high voltage; meanwhile, the initial impedance of the battery cell is improved, the impedance growth rate is reduced, the battery capacity recovery rate is improved, the gas production problem is inhibited, and the high-temperature storage performance and long-term cycle stability of the battery are improved.

The present invention provides an alkylthioaryl clavudine phosphoramide compound, as shown in formula (I). Compared with the prior art, the alkylthioaryl clavudine phosphoramide compound provided by the present invention retains a unique anti-HBV activity while reducing the systemic exposure of clavudine, eliminating possible toxic side effects, providing a new option for combined drug use, and may provide a new functional cure solution for HBV patients in the future.

The invention relates to the technical field of food processing, in particular to an efficient processing method for rhodomyrtus tomentosa functional food.The efficient processing method comprises the following steps that S1, raw material smashing is conducted, specifically, raw materials are washed with clear water for 5-10 min, and the washed raw materials are added into a processing device to be smashed for 10-15 min; s2, grinding the raw materials: grinding the crushed raw materials for 10-15 minutes; s3, filtering the raw materials: filtering the ground raw materials according to the filtering standard of a 150-200-mesh sieve to obtain raw material juice; s4, enzymolysis of the raw material juice: adding a mixed degrading enzyme into the raw material juice according to a mass ratio of 1: 10 to obtain a semi-finished product; s5, semi-finished product fermentation: the semi-finished product is sealed and placed for 1-2 days for fermentation, and fermentation liquor is obtained; s6, semi-finished product distillation: after fermentation is completed, heating fermentation liquor to 50-52 DEG C under the pressure of 1500-1600 Pa, and evaporating guaiacol glyceryl ether in the fermentation liquor; and S7, obtaining a finished product. The processing efficiency of the rhodomyrtus tomentosa functional food can be effectively improved, and the stirring, grinding and fermentation processes are integrated.

The invention discloses a preparation method of amphotericin B sustained release microspheres, which comprises the following steps: S1, suspending AmB in methanol to prepare an AmB suspension; s2, dissolving BSA in water to prepare a BSA aqueous solution; s3, uniformly mixing the AmB suspension with the BSA aqueous solution to obtain a water phase; s4, taking liquid paraffin as an oil phase, adding an emulsifier, then adding the water phase into the oil phase under a mechanical stirring condition, emulsifying to obtain W / O type primary emulsion, and naturally volatilizing to remove methanol; s5, a glutaraldehyde solution with the concentration being 8 wt% is added into the primary emulsion for curing; s6, the cured substance is washed and freeze-dried, and the amphotericin B sustained release microspheres are prepared. The amphotericin B sustained-release microsphere can reduce the administration frequency, alleviate the dose-dependent toxic and side effects and reduce the cost of pharmaceutic adjuvants and preparations.

The invention discloses an application of an intestinal strain in preparation of a cholesterol-lowering product, and belongs to the technical field of microbial medicines. The intestinal bacterial strain is Oscillibacter ruminant, the viable bacteria concentration of the intestinal bacterial strain is controlled to be (4.8-5.2) * 10 < 9 > CFU / mL, the intestinal bacterial strain can be prepared into bacterial liquid, freeze-dried powder, capsules or tablets and other dosage forms, and pharmaceutically acceptable auxiliary materials such as lactose and microcrystalline cellulose can be added. According to the cholesterol-lowering product, the total cholesterol, triglyceride and low-density lipoprotein cholesterol level of an object can be remarkably reduced under the action of peste des petits ruminants, and meanwhile, the high-density lipoprotein cholesterol level is increased; in addition, abnormal blood glucose induced by high-fat diet can be improved, arterial lipid accumulation is reduced, and the plaque area proportion of atherosclerotic lesions is improved.

The invention discloses a preparation method of drug delivery nanoparticles based on hydrogel microneedles, and relates to the technical field of biological medicine, the technical key points are as follows: step S1, preparing a white suspension solution; step S2, preparing a white powdery porous hollow nano microsphere A; step S3, preparing A nano microsphere powder loaded with TP; step S4, preparing a CaCO3 (at) Lip (at) DEC205 nano microsphere preparation; and S5, preparing the PVP microneedle: dropwise adding a proper amount of polyvinylpyrrolidone solution into the mold, keeping the weights of the two six-hole culture plates equal, centrifuging, putting the obtained culture plates into a drying oven, evaporating the solution to dryness, and taking down the prepared microneedle from the mold. The liposome is added, so that the TP drug loading efficiency is higher, and the toxic and side effects of the whole body are reduced.

The invention discloses a photo-thermal and hypoxia dual-sensitive nano carrier material and a preparation method and application thereof, and relates to the field of nano materials, the preparation method comprises the following steps: S1, preparing a carboxymethyl chitosan grafted azobenzene product CMCS-Azo; s2, respectively preparing an L-glutamic acid solution and a carboxymethyl chitosan grafted azobenzene nanoparticle solution, then stirring the L-glutamic acid solution and the carboxymethyl chitosan grafted azobenzene nanoparticle solution for reaction, and performing post-treatment on an obtained reaction solution b to obtain L-Gr-CMCS-Azo; s3, adding the L-Gr-CMCS-Azo into the DMF solution in which the metal compound is dissolved, carrying out ultrasonic treatment and stirring, carrying out post-treatment on the obtained reaction solution c, and finally, carrying out vacuum drying to obtain a solid L-Gr-CMCS-Azo (at) M; and S4, dissolving the L-Grub-CMCS-Azo (at) M material in a DMSO solution, carrying out ultrasonic assisted dissolution, slowly dropwise adding into deionized water, stirring at a low speed, filtering with a microfiltration membrane, and freeze-drying to obtain the nano-carrier material. Therefore, the photo-thermal and hypoxia dual-sensitive nano-carrier material as well as the preparation method and the application thereof are of great significance to the development of anti-cancer therapy.

The invention belongs to the technical field of medicine, and provides an external pharmaceutical composition for waist training injury pain and a patch thereof, the external pharmaceutical composition is prepared from scutellaria baicalensis, angelica sinensis, poria cocos, salvia miltiorrhiza, radix angelicae pubescentis, golden cypress, ligusticum wallichii, frankincense, garden balsam stem, safflower, galangal, liquorice and diclofenac sodium. According to the external medicine composition, the medicines generate a mutual synergistic effect in a manner of combined application of traditional Chinese medicines and western medicines, so that the purpose of enhancing the medicine effect is achieved, and the analgesic effect is remarkable. The combined action of Chinese and western medicines alleviates the side effect of pure western medicines and improves the problem of unstable curative effect of pure traditional Chinese medicines. The external medicine composition is high in safety, free of stimulation, exact in curative effect, free of pain in treatment, high in clinical cure rate and easy to accept by patients after being used for a long time.

The invention particularly relates to a glycosylated dendrimer for co-delivery of small interfering RNA (Ribonucleic Acid) and small molecular drugs as well as a preparation method and application of the glycosylated dendrimer. The nano platform provided by the invention is characterized in that an anti-inflammatory drug resveratrol (Res) and electrostatic adsorption small interfering RNA (si-Cp2e1) are loaded in a glycosylated dendrimer G5-Gal. The preparation method and the application comprise the following steps: preparation of G5-Gal, preparation of Res (at) G5-Gal, and preparation of Res (at) G5-Gal / si-Cp2e1. The preparation method is simple, the required raw materials are easy to obtain, the synthesis process is simple and convenient, separation and purification are easy, and the synthesized nano-drug has a liver targeting capability, can realize dual treatment of small molecule drug-small interfering RNA, and has a potential application prospect in the aspect of treatment of alcohol-related liver diseases.

The invention discloses a choline polymer as well as a preparation method and application thereof. The copolymer comprises an ethylenically unsaturated monomer unit with choline and / or a derivative thereof and a hydrophilic (methyl) acrylic acid monomer unit. According to the invention, choline and a derivative monomer thereof are introduced into the copolymer, so that the copolymer has cationic polymer properties and liver efficient specific targeting properties. Meanwhile, the choline polymer can be modified on the surface of drug-loaded nanoparticles (such as lipid nanoparticles), and can also form a cation-drug compound through a cation unit of choline and drug molecules, so that drug leakage in the storage process and early release of the drug in blood circulation are greatly reduced, and the bioavailability of the drug is improved. Toxic and side effects of the medicine are reduced, and the medicine is efficiently targeted and delivered to the liver.

The present invention relates to glucagon and GLP-1 co-agonist compounds that are useful in the treatment of type 2 diabetes, obesity, non-alcoholic fatty liver disease (NAFLD) and / or non-alcoholic steatohepatitis (NASH) p-20637