68 results about "Human papillomavirus" patented technology

The invention discloses an HPV whole genome stable strain as well as a preparation method and application thereof, and relates to the field of gene recombination. The invention provides a method for constructing an HPV (human papillomavirus) whole genome stable strain by utilizing a PiggyBac transposon system. The sequence of an HPV whole genome is shown as SEQ ID NO. 2. The HPV whole genome stable strain obtained by the method has high stability and high copy number, and can be used as an HPV genome cell quality control product.

The invention discloses pyridone endoperoxide with antibacterial and antiviral activity and application thereof, and belongs to the fields of organic compound synthesis and medical technology, food engineering and the like. The invention proves that the series of endoperoxides can effectively kill pathogenic bacteria, fungi and viruses, including propionibacterium acnes, escherichia coli, staphylococcus aureus, candida albicans, human papilloma viruses, herpes simplex viruses and the like. The internal peroxide designed by the invention has the capability of treating skin diseases, is expected to be developed into a first-line medicine for treating skin diseases caused by pathogen invasion, and mainly relates to bacterial skin diseases, fungal skin diseases and viral skin diseases caused by pathogen infection. Meanwhile, the series of endoperoxides can be used for treating oral infection, periodontitis, infection of shoulder and other joint operations, and bacterial infection such as pressure sores. In addition, the peroxides in the series can also be used in the fields of food preservation, disinfectant preparation and the like.

The present invention relates to a biological sample self-collection device that houses a collector (such as a “veil”, buffer, swab, brush, etc.) for any biological material, preferably of vaginal origin. At the same time, it is designed to combine a reactive liquid with said substances for subsequent analysis thereof in a watertight manner. Its use includes the detection of pathogens, such as human papillomavirus, and the diagnosis of cervical cancer, among others.

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

The invention discloses an oral composition for preventing and treating HPV (human papillomavirus) based on enhancement of lower jiao immunity and application of the oral composition. The traditional Chinese medicine composition is prepared from, by weight, 1-3 parts of cinnamon, 3-10 parts of radix paeoniae rubra, 3-10 parts of peach kernels, 3-10 parts of cortex moutan, 3-10 parts of poria cocos, 5-15 parts of raw radix astragali, 1-3 parts of ginseng, 3-10 parts of rhizoma atractylodis macrocephalae, 1-3 parts of liquorice, 3-10 parts of rhizoma atractylodis, 3-10 parts of cortex phellodendri, 3-10 parts of radix cyathulae, 3-10 parts of semen coicis, 3-10 parts of thunberg fritillary bulb, 3-10 parts of honeysuckle, 3-10 parts of rhizoma smilacis glabrae, 3-10 parts of herba lycopi, 3-10 parts of herba leonuri and 3-10 parts of eucommia ulmoides. 3-10 parts of semen cuscutae, 3-10 parts of fennel, 3-10 parts of Chinese yam, 3-10 parts of radix linderae and 3-10 parts of rhizoma cyperi; through dual mechanisms of supporting healthy energy and eliminating evil and detoxifying, HPV viruses are effectively eliminated, the progress of lesion is blocked, and the traditional Chinese medicine composition has the advantages of various dosage forms, safety and mildness.

Methods for treating human papillomavirus (HPV) type 16- or HPV type 18-associated high-grade squamous intraepithelial lesions (HSIL) of the cervix are described

The invention discloses an anti-HPV (human papillomavirus) gel dressing and a preparation method thereof, and belongs to the technical field of dressings, and the preparation method comprises the following steps: dissolving lopinavir in methanol to obtain an organic phase, adding poloxamer 407 into deionized water to obtain a water phase, dropwise adding the organic phase into the water phase at a constant speed while stirring under a room temperature condition, and after dropwise adding is finished, performing suction filtration and drying to obtain the anti-HPV gel dressing. Stirring in a fuming cupboard to volatilize methanol, and carrying out particle size treatment on the obtained solution by using a high-pressure homogenizer to obtain a lopinavir nanoparticle solution; the preparation method comprises the following steps: adding a chitosan-based functional component, poloxamer 407 and poloxamer 188 into a lopinavir nanoparticle solution, stirring at the temperature of 4 DEG C and the speed of 100-200r / min for 2-4 hours, and treating in a refrigerator at the temperature of 4 DEG C for 48 hours to obtain the anti-HPV gel dressing, the anti-HPV gel dressing has good drug sustained release performance, mucous membrane adhesion and viscosity permeability, and the bioavailability of lopinavir is greatly improved.

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

The utility model relates to the technical field of hypochlorous acid gel production, in particular to a stirring mechanism for anti-HPV (human papillomavirus) carbomer hypochlorous acid gel production, which comprises a mixing box, a top plate, a feeding pipe, a discharging pipe and a staged quantitative stirring component, the staged quantitative stirring component comprises an object placing plate, a weighing column, a spring and a storage box, the top plate is detachably connected with the mixing box, and the top plate is detachably connected with the storage box. The feeding pipe is detachably connected with the top plate and located on the upper portion of the interior of the top plate, the discharging pipe is detachably connected with the mixing box, the storage plate is fixedly connected with the feeding pipe, the weighing column is detachably connected with the storage plate, the spring is arranged on the outer surface of the weighing column, and the storage box is detachably connected with the weighing column. Therefore, on the premise that the stirring and mixing quality is guaranteed, the problems that when carbomer hypochlorous acid gel is produced, graded input cannot be carried out, various materials are prone to being left in a pipeline of the feeding port at the same time, and the production quality is affected are effectively solved.

The present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine. In particular, the present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine, which is useful for topical application. The present invention also provides the use of such compositions for medical conditions in the keratinizing and non-keratinizing epithelium / skin, such as the treatment of human papillomavirus (HPV)-related pre-cancerous conditions.

The invention discloses a construction method and application of an animal model for HPV (human papillomavirus) pathogenic mechanism research, and belongs to the technical field of animal models. According to the construction method of the HPV fixed-point integration animal model, HPV16 gene knock-in is carried out on the Col1a1 gene locus of a C57BL / 6J mouse through CRISPR / Cas mediated genome engineering. The method comprises the following steps: (1) designing specific gRNA; (2) constructing a knock-in vector Donor vector; and (3) uniformly mixing Cas9 protein, gRNA and a targeting vector Donor vector to obtain an injection compound, performing micro-injection on the injection compound into a mouse fertilized egg, and sending the injection compound back to a pregnant mouse fallopian tube to cultivate offspring so as to generate a gene knock-in mouse. The animal model prepared by the method can be used as a contrast of a cervical cancer precancerous lesion animal model for scientific research.

The utility model discloses an HPV (human papillomavirus) detection sampling tube and belongs to the technical field of HPV detection. Comprising a sampling pipe, the top of the sampling pipe is provided with a push rod, the bottom of the push rod is provided with a rubber block, and the circumferential outer wall of the rubber block is provided with a plurality of sealing rings. After the swab is used for sampling, the fixed ball at the top of the swab is embedded into the bottom of the mounting seat, then the swab is inserted into the sampling tube and is fully mixed with detection liquid in the sampling tube, the rubber plug is separated from the liquid outlet nozzle by rotating the sealing block, then the push rod is pushed, the detection liquid in the sampling tube is squeezed out, and detection is directly carried out; the detection liquid can be prevented from being in contact with air to cause pollution to the detection liquid; the symmetrically arranged rubber sheets can buffer the shell, the rubber sheets are arc-shaped, so that the buffering effect of the shell is better, and meanwhile, the spring arranged at the top of the base can buffer the shell again, so that the sampling tube can be prevented from being damaged when the sampling tube falls off.

The utility model relates to the technical field of HPV (human papillomavirus) detection devices, in particular to an HPV detection sample storage device which comprises a storage box and a storage mechanism, quick release structures are respectively arranged at the central positions of the inner walls of the front side and the rear side of the storage box, and the storage box is connected with the storage mechanism in a quick release manner through the two quick release structures. Clamping mechanisms are symmetrically arranged on the upper portions of the outer walls of the left side and the right side of the storage box correspondingly, limiting assemblies are symmetrically arranged on the lower portions of the outer walls of the left side and the right side of the storage box correspondingly, and during use, storage mechanisms containing sponge blocks of different sizes can be rapidly installed in the storage box through mutual cooperation of all structures in a rapid disassembly structure; and the stability of the storage mechanism mounted in the inner cavity of the storage box is ensured.

The invention provides an HPV (human papillomavirus) neutralizing antibody pseudovirus as well as a preparation method and application thereof, and belongs to a transgenic technology. The method comprises the following steps: firstly, constructing a 293TT cell strain capable of stably and highly expressing SV40 large T antigen, wherein the 293TT cell strain is used as an HPV (human papillomavirus) neutralizing antibody pseudovirus packaging tool cell; then fusing a 6X His tag at the N end or the C end of the L1 and L2 genes of the main capsid protein of the HPV virus to construct plasmids; the constructed plasmids and reporter gene plasmids jointly transfect 293TT cells, and after transfection, a Ni column is combined with a His tag for purification to obtain the neutralizing antibody pseudovirus. The pseudovirus is purified by using the Ni column, which is simple and efficient; the purified pseudovirus is high in purity, high in infection activity and complete in structure, and the particle size of the pseudovirus is consistent with that of the HPV live virus, and the structure is similar to that of the HPV live virus.

Disclosed herein is an immunotherapy for human papillomavirus. More particular, disclosed is a therapeutic composition that includes one or more polypeptides that comprise a plurality of epitopes derived from multiple human papillomavirus antigens, which, when used in immunotherapy is capable of eliciting a protective and durable cell mediated immune response, without being limited thereto. Also disclosed are methods of treating or preventing HPV and / or cancer.

This invention is directed to immunogenic composition, conjugates, virus-like particles (VLP) compositions, vaccines and methods directed to the treatment and / or prevent of infection by Human Papillomavirus.

The present invention provides a primer-probe combination for detecting HPV and / or HPV typing, belonging to the field of human papillomavirus (HPV) detection technology. The primer-probe combination includes one or more of HPV16E7, HPV18E7, HPV31E7, HPV45E7, HPV33E7, HPV35E7, HPV59E6, HPV68E6, HPV58E7, HPV51E6, HPV52E7, HPV56E6, HPV66E6, and HPV39E6, and can distinguish different types of HPV, with good specificity, accuracy, and low detection limit. The present invention uses a digital PCR method (dPCR) to detect the fluorescent signal of each droplet, and calculates the copy number of the target gene fragment in the entire reaction system based on the Poisson distribution principle, so that absolute quantification can be achieved without relying on a standard curve.

The invention relates to the technical field of HPV (human papillomavirus) detection, and discloses an HPV rapid detection method based on an optimized CRISPR-Cas13a platform, which comprises the following steps: step S1, splitting and purifying a sample to obtain an HPV DNA (deoxyribonucleic acid) template; s2, performing amplification and transcription on the HPV target nucleic acid to obtain an amplification product; step S3, mixing an amplification product with a detection system of CRISPR-Cas13a protein, crRNA and reporter molecules, and then carrying out a cleavage reaction; and step S4, detecting the state change of the reporter molecule through a lateral flow chromatography test strip so as to judge whether the HPV exists in the sample or not. According to the invention, the recombinase polymerase is combined with CRISPR-Cas13a, so that the process from nucleic acid amplification to signal detection is completed at a single and mild constant temperature, the operation complexity and environmental requirements are remarkably reduced, and the HPV can be rapidly detected.

The invention relates to the related technical field of anti-HPV bioprotein gel production, in particular to an anti-HPV bioprotein gel preparation method and an anti-HPV bioprotein gel preparation device, the anti-HPV bioprotein gel preparation device comprises a preparation machine, and a fixing mechanism is arranged in the preparation machine. According to the preparation method and the preparation device of the anti-HPV biological protein gel, the fixing mechanism is arranged, and a sealing ring, a first fixing block, a nut, a second fixing block and a threaded rod are matched for use, so that a firmer fixing effect is realized, various problems caused by untight fixing are effectively prevented, and the cleaning mechanism is arranged, so that the anti-HPV biological protein gel can be conveniently cleaned. Through cooperative work of a water pump, a water inlet, a water outlet pipe, a fixing groove, an annular pipe and a nozzle, cleaning of the interior of the stirring tank is achieved, through a sealing mechanism, efficient sealing of the stirring tank is achieved through cooperative work of a first fixing plate, a limiting plate, a bolt, a first sealing plate, a second fixing plate, a sealing ring and a second sealing plate, and the sealing effect of the stirring tank is improved. The cleanliness and the safety of a preparation environment are greatly improved.

Provided are lipid particles encapsulating a nucleic acid capable of expressing an E6 antigen and an E7 antigen of human papillomavirus, whereby a vaccine for preventing and / or treating infection with human papillomavirus type 6 and / or type 11 can be provided. The lipid particles comprise a lipid that is a cationic lipid having the general formula (Ia), or a pharmaceutically acceptable salt thereof.[In the formula, R1, R2, p, L1 and L2 are as defined in the specification.]

The invention discloses a plant composite antibacterial liquid composition aiming at HPV (human papillomavirus) infection and a preparation method thereof, and belongs to the technical field of biological medicines. The composition is prepared from the following components in parts by weight: 5 to 20 parts of brucea javanica extract, 3 to 15 parts of senna extract, 2 to 10 parts of anhydridized beta-lactoglobulin, 1 to 8 parts of dandelion extract, 1 to 5 parts of penetration enhancer, 0.1 to 1 part of pH (Potential of Hydrogen) regulator, 0.1 to 0.5 part of preservative and the balance of phosphate buffer solution. According to the invention, anhydridized beta-lactoglobulin is innovatively taken as a carrier, so that targeted delivery of active ingredients is realized; the anti-HPV effect is enhanced through the synergistic effect of the brucea javanica and the kalii extract; the anti-inflammatory repair function of the dandelion extract is combined, and a compound penetration enhancer and a specific preparation process are matched, so that the product has the characteristics of efficient HPV resistance, anti-inflammatory repair, mildness, no irritation and good stability.

The present invention relates to a pharmaceutical composition comprising an immunoglobulin Fc region and an IL-7 fusion protein. Specifically, when a fusion protein comprising the immunoglobulin Fc region and IL-7 is administered to an affected area, a strong immune response is induced in the body and thus allows human papillomavirus-caused diseases to be prevented or treated.

This invention relates to antisense oligonucleotides targeting different subtypes of human papillomavirus (HPV) oncogenes and their applications. The ASO molecules are 14–40 bases in length and specifically pair with specific regions of target genes. This invention also relates to the following applications of the ASO molecules: (1) preparing preparations to inhibit HPV virus expression; or (2) preparing drugs or drug compositions for treating diseases related to HPV infection; or (3) treating diseases related to HPV infection; or (4) inhibiting the expression of HPV virus genes.

The present invention provides a method of treating an infectious disease. The method comprises the step of administering to a subject in need thereof an effective amount of (i) a polymer-flavonoid conjugate, (ii) a flavonoid oligomer, or (iii) a micelle having a shell formed from one or more polymer-flavonoid conjugates or one or more flavonoid oligomers, or a combination thereof and having an agent encapsulated within the shell. The methods of the present invention can effectively treat viral infections, such as, for example; severe acute respiratory syndrome coronavirus (SARS-CoV), enterovirus, HIV, hepatitis B virus, MERS-CoV, influenza virus, dengue fever virus, respiratory syncytial virus, hepatitis C virus, monkey pox virus, human papilloma virus, methicillin-resistant staphylococcus aureus, pseudomonas, tuberculosis, bacillus anthracis, tetanus, streptococcus pneumoniae, meningococcus, escherichia coli, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, bacillus subtilis, , Legionella genus, Neisseria gonorrhoeae, Neisseria meningitidis, and Salmonella genus.

The utility model relates to the technical field of injectors, and discloses an injector for injection of anti-HPV biological protein dressing, which comprises a tube body, one end of the surface of the tube body is in threaded connection with a protective cover, one end of the tube body is provided with an injection hole, the inner wall of the tube body is in sliding connection with a push rod, one end of the push rod is fixedly connected with a piston, and the surface of the tube body is provided with round holes. A limiting ring and a recycling pipe are fixedly connected to the surface of the pipe body, a recycling groove and a discharging hole are formed in the surface of the recycling pipe, a shell is fixedly connected to one side of the limiting ring, connecting holes matched with the recycling groove and the discharging hole are formed in the surface of the shell, and an adjusting ring is rotationally connected between the shell and the recycling pipe. Via holes are formed in the surfaces of the adjusting rings, and limiting mechanisms are fixedly installed on the surface of the shell. According to the injector for injection of the anti-HPV biological protein dressing, the anti-HPV biological protein dressing can be conveniently and evenly smeared on the inner wall of the vagina, meanwhile, secretions in the vagina can be cleaned, and the drug effect of the anti-HPV biological protein dressing is improved.

The utility model discloses a sampling test tube for human papilloma virus inhibitor detection and analysis, which comprises a test tube body, the outer surface of the test tube body is fixedly connected with a connecting table at the upper edge position, the bottom end of the connecting table is in threaded connection with a protective jacket, and the outer surface of the protective jacket is fixedly communicated with an exhaust tube. And the bottom end of the exhaust pipe fixedly communicates with an adjusting pipe. Through the arrangement of the protective jacket, the test tube body can be effectively protected, so that the test tube body can be effectively prevented from being directly damaged when collision occurs, the service life is prolonged, and furthermore, a vacuum pump can be connected with an exhaust pipe to extract air between the test tube body and the protective jacket to form a vacuum environment; a sample in the test tube body is difficult to be interfered by external air temperature, and the quality of the sample is improved.

The invention relates to a recombinant bioactive protein for preventing or treating human papilloma virus infection as well as a preparation method and application thereof, and belongs to the technical field of medicines. The recombinant bioactive protein disclosed by the invention is anhydridized recombinant lactoferrin, anhydridized recombinant lactoglobulin or anhydridized recombinant histone; the recombinant bioactive protein is obtained by carrying out anhydridation modification on recombinant lactoferrin, recombinant lactoglobulin or recombinant histone by using 3-hydroxyphthalic anhydride or a derivative thereof, 4-hydroxyphthalic anhydride or a derivative thereof, succinic anhydride or maleic anhydride. The bioactive protein disclosed by the invention has the advantages of preventing HPV virus from invading cells, preventing virus from expanding infection, resisting bacteria and oxidation, being safe, stable, convenient to store and transport, low in cost and the like.

This invention provides a non-classical crRNA, reagent, kit, and application for one-step RPA-Cas12a nucleic acid detection. Based on classic crRNA, the non-classical crRNA shortens the 5' end by two bases and / or adjusts the stem-loop base structure to one of UCUUUU, UCUUU, UGUUU, UGUU, or UAAGU. This non-classical crRNA structure reduces the affinity of the Cas12a-crRNA complex for the target DNA, weakens the cis-cleavage activity of Cas12a, and increases the trans-cleavage activity of Cas12a, without requiring additional base modifications. This invention achieves a detection limit of 1 fM in the detection of Group B Streptococcus and Human Papillomavirus 16, with a sensitivity approximately 10-fold higher than before optimization.

The invention provides an HPV (human papillomavirus) typing primer probe composition and application thereof, and relates to the technical field of biology. The HPV typing primer probe composition comprises specific primer pairs aiming at the following 23 types of HPV types: HPV6, HPV11, HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV42, HPV43, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV81 and HPV82, and the nucleotide sequences of the specific primer pairs are shown as SEQ ID NO.1-15 and SEQ ID NO.18-40. According to the HPV typing primer probe composition, the defects that an existing HPV typing product is limited in HPV type distinguishing capacity and tedious in operation are overcome.