129 results about "Human papillomavirus" patented technology

The invention discloses an HPV whole genome stable strain as well as a preparation method and application thereof, and relates to the field of gene recombination. The invention provides a method for constructing an HPV (human papillomavirus) whole genome stable strain by utilizing a PiggyBac transposon system. The sequence of an HPV whole genome is shown as SEQ ID NO. 2. The HPV whole genome stable strain obtained by the method has high stability and high copy number, and can be used as an HPV genome cell quality control product.

The invention discloses pyridone endoperoxide with antibacterial and antiviral activity and application thereof, and belongs to the fields of organic compound synthesis and medical technology, food engineering and the like. The invention proves that the series of endoperoxides can effectively kill pathogenic bacteria, fungi and viruses, including propionibacterium acnes, escherichia coli, staphylococcus aureus, candida albicans, human papilloma viruses, herpes simplex viruses and the like. The internal peroxide designed by the invention has the capability of treating skin diseases, is expected to be developed into a first-line medicine for treating skin diseases caused by pathogen invasion, and mainly relates to bacterial skin diseases, fungal skin diseases and viral skin diseases caused by pathogen infection. Meanwhile, the series of endoperoxides can be used for treating oral infection, periodontitis, infection of shoulder and other joint operations, and bacterial infection such as pressure sores. In addition, the peroxides in the series can also be used in the fields of food preservation, disinfectant preparation and the like.

The utility model discloses a medical instrument for treating cervicitis and cervical HPV (human papillomavirus) infection, which comprises a liquid medicine storage bag in which treatment medicine is stored, a conveying pipeline is fixedly arranged outside the front end of the liquid medicine storage bag, and a spraying head is arranged at the top end of the conveying pipeline. The spraying head is used for spraying out treatment liquid medicine to achieve the purpose of treating cervicitis and cervical HPV infection, handheld finger rings are installed on the left side and the right side of the root of the conveying pipeline, an installation ring is installed outside the conveying pipeline, and expansion mechanisms are installed on the left side and the right side of the installation ring. According to the medical instrument for treating cervicitis and cervical HPV infection, a novel structural design is adopted, an expansion mechanism is arranged, expansion plates on the left side and the right side of a conveying pipeline enter the vagina of a patient along with the device in the using process of the device, then the expansion plates on the left side and the right side rotate outwards, the vagina of the patient is expanded, and therefore the vagina of the patient is expanded. Therefore, friction between the conveying pipeline and the body of a patient when the conveying pipeline extends into the body is reduced, and discomfort of the patient is relieved.

The present invention relates to a biological sample self-collection device that houses a collector (such as a “veil”, buffer, swab, brush, etc.) for any biological material, preferably of vaginal origin. At the same time, it is designed to combine a reactive liquid with said substances for subsequent analysis thereof in a watertight manner. Its use includes the detection of pathogens, such as human papillomavirus, and the diagnosis of cervical cancer, among others.

The invention belongs to the technical field of medicines, and particularly relates to anti-HPV (human papillomavirus) earthworm extract carrier gel as well as a preparation method and application thereof. The preparation method comprises the following steps: stirring and hydrating an earthworm extract and a lipid layer to obtain lipid vesicles, then carrying out expansion, ultrasonic treatment and extrusion to obtain an earthworm extract carrier, and finally adding pH-adjusted carbomer and uniformly mixing to obtain the anti-HPV earthworm extract carrier gel. The Carbomer gel is compounded with the earthworm extract carrier, so that the inhibiting effect on HPV (human papillomavirus) is improved, cervical cancer cells can be promoted to shrink and die, the gel is combined with the earthworm extract carrier, the viscosity of the earthworm extract carrier is further improved, the fitting property of the earthworm extract carrier and the inner wall of the vagina is improved, and the effect of treating cervical cancer is achieved. Therefore, the effect of preventing and treating HPV virus infection is improved.

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

The utility model relates to the technical field of kits, in particular to an efficient HPV (human papillomavirus) typing rapid detection kit which comprises a kit body, and a kit cover is mounted on the outer wall of the kit body; the arc-shaped convex edge drives the sealing adhesive tape to be torn off, the sampling brush in the clamping groove is taken, after the sampling brush extracts a sample, the liquid storage bottle body is opened, the extracted sample is mixed with liquid in the liquid storage bottle body, and then the dropper is used for dropping the mixed liquid onto the pyrophosphoric acid test plate and the DNA test plate for determination. DNA bar code technology detection and pyrosequencing technology detection can be directly carried out, operation is easy and convenient, the step of taking packaging bags is omitted, and the whole detection process is faster; the medical waste in the inner cavity of the sealing groove is sealed by inserting the sealing rubber plug into the end opening of the sealing groove, the box body is not easily punctured by the sharp part of the sampling brush, and then the box body is placed in the inner cavity of the recycling bag, so that the medical waste is further sealed, and the pollution to the environment is prevented.

The invention discloses an oral composition for preventing and treating HPV (human papillomavirus) based on enhancement of lower jiao immunity and application of the oral composition. The traditional Chinese medicine composition is prepared from, by weight, 1-3 parts of cinnamon, 3-10 parts of radix paeoniae rubra, 3-10 parts of peach kernels, 3-10 parts of cortex moutan, 3-10 parts of poria cocos, 5-15 parts of raw radix astragali, 1-3 parts of ginseng, 3-10 parts of rhizoma atractylodis macrocephalae, 1-3 parts of liquorice, 3-10 parts of rhizoma atractylodis, 3-10 parts of cortex phellodendri, 3-10 parts of radix cyathulae, 3-10 parts of semen coicis, 3-10 parts of thunberg fritillary bulb, 3-10 parts of honeysuckle, 3-10 parts of rhizoma smilacis glabrae, 3-10 parts of herba lycopi, 3-10 parts of herba leonuri and 3-10 parts of eucommia ulmoides. 3-10 parts of semen cuscutae, 3-10 parts of fennel, 3-10 parts of Chinese yam, 3-10 parts of radix linderae and 3-10 parts of rhizoma cyperi; through dual mechanisms of supporting healthy energy and eliminating evil and detoxifying, HPV viruses are effectively eliminated, the progress of lesion is blocked, and the traditional Chinese medicine composition has the advantages of various dosage forms, safety and mildness.

Methods for treating human papillomavirus (HPV) type 16- or HPV type 18-associated high-grade squamous intraepithelial lesions (HSIL) of the cervix are described

The invention discloses an anti-HPV (human papillomavirus) gel dressing and a preparation method thereof, and belongs to the technical field of dressings, and the preparation method comprises the following steps: dissolving lopinavir in methanol to obtain an organic phase, adding poloxamer 407 into deionized water to obtain a water phase, dropwise adding the organic phase into the water phase at a constant speed while stirring under a room temperature condition, and after dropwise adding is finished, performing suction filtration and drying to obtain the anti-HPV gel dressing. Stirring in a fuming cupboard to volatilize methanol, and carrying out particle size treatment on the obtained solution by using a high-pressure homogenizer to obtain a lopinavir nanoparticle solution; the preparation method comprises the following steps: adding a chitosan-based functional component, poloxamer 407 and poloxamer 188 into a lopinavir nanoparticle solution, stirring at the temperature of 4 DEG C and the speed of 100-200r / min for 2-4 hours, and treating in a refrigerator at the temperature of 4 DEG C for 48 hours to obtain the anti-HPV gel dressing, the anti-HPV gel dressing has good drug sustained release performance, mucous membrane adhesion and viscosity permeability, and the bioavailability of lopinavir is greatly improved.

The utility model provides an HPV (human papillomavirus) detection kit, which belongs to the technical field of kits and comprises a first kit body. A mounting frame is mounted at the upper end of the first box body through bolts, a groove is formed in the mounting frame, a top plate is mounted in the groove through bolts, two sliding grooves are formed in the top plate, two toothed plates are mounted at the lower end of the top plate through screws, two sliding seats are mounted at the upper end of the top plate, and two round rods are fixed to the lower ends of the sliding seats; the positioning groove is formed in the positioning plate, the upper end of the storage pipe is positioned through the positioning groove, the supporting block is installed on the connecting base through the bolt, the groove is formed in the supporting block, the lower end of the storage pipe is located in the groove in the supporting block, the lower end of the storage pipe is positioned through the groove in the supporting block, and the storage pipe is prevented from shaking; and the stability of the storage pipe is improved.

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

The utility model relates to the technical field of hypochlorous acid gel production, in particular to a stirring mechanism for anti-HPV (human papillomavirus) carbomer hypochlorous acid gel production, which comprises a mixing box, a top plate, a feeding pipe, a discharging pipe and a staged quantitative stirring component, the staged quantitative stirring component comprises an object placing plate, a weighing column, a spring and a storage box, the top plate is detachably connected with the mixing box, and the top plate is detachably connected with the storage box. The feeding pipe is detachably connected with the top plate and located on the upper portion of the interior of the top plate, the discharging pipe is detachably connected with the mixing box, the storage plate is fixedly connected with the feeding pipe, the weighing column is detachably connected with the storage plate, the spring is arranged on the outer surface of the weighing column, and the storage box is detachably connected with the weighing column. Therefore, on the premise that the stirring and mixing quality is guaranteed, the problems that when carbomer hypochlorous acid gel is produced, graded input cannot be carried out, various materials are prone to being left in a pipeline of the feeding port at the same time, and the production quality is affected are effectively solved.

The present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine. In particular, the present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine, which is useful for topical application. The present invention also provides the use of such compositions for medical conditions in the keratinizing and non-keratinizing epithelium / skin, such as the treatment of human papillomavirus (HPV)-related pre-cancerous conditions.

The invention discloses a construction method and application of an animal model for HPV (human papillomavirus) pathogenic mechanism research, and belongs to the technical field of animal models. According to the construction method of the HPV fixed-point integration animal model, HPV16 gene knock-in is carried out on the Col1a1 gene locus of a C57BL / 6J mouse through CRISPR / Cas mediated genome engineering. The method comprises the following steps: (1) designing specific gRNA; (2) constructing a knock-in vector Donor vector; and (3) uniformly mixing Cas9 protein, gRNA and a targeting vector Donor vector to obtain an injection compound, performing micro-injection on the injection compound into a mouse fertilized egg, and sending the injection compound back to a pregnant mouse fallopian tube to cultivate offspring so as to generate a gene knock-in mouse. The animal model prepared by the method can be used as a contrast of a cervical cancer precancerous lesion animal model for scientific research.

The invention discloses application of a traditional Chinese medicine composition for strengthening body resistance, eliminating evil and treating HPV infection, and belongs to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises the following raw materials: 40-50 parts of astragalus membranaceus, 40-50 parts of codonopsis pilosula slices, 40-50 parts of rhizoma smilacis glabrae, 25-45 parts of rehmannia, 10-20 parts of fructus cnidii and 10-20 parts of radix sophorae flavescentis. The traditional Chinese medicine composition is mainly applied in the form of a traditional Chinese medicine external preparation, and can be used for treating one of low-risk HPV infection, high-risk HPV infection and mixed HPV infection and various diseases caused by HPV infection. The traditional Chinese medicines such as astragalus membranaceus, codonopsis pilosula and the like for tonifying healthy qi are matched with the traditional Chinese medicines such as rhizoma smilacis glabrae, fructus cnidii, sophora flavescens, rehmannia glutinosa and the like for clearing away heat and toxic materials and eliminating evil, so that the traditional Chinese medicine composition can be exactly used for the symptoms of qi deficiency and exogenous infection such as HPV (human papillomavirus) infection or qi and blood deficiency caused by interior excess and heat accumulation, and the principle of strengthening healthy qi and eliminating evil is adopted, so that pathogenic factors are attacked without damaging healthy qi, the curative effect can be improved, and disease relapse can be inhibited; and side effects are reduced.

The utility model discloses an HPV (human papillomavirus) detection sampling tube and belongs to the technical field of HPV detection. Comprising a sampling pipe, the top of the sampling pipe is provided with a push rod, the bottom of the push rod is provided with a rubber block, and the circumferential outer wall of the rubber block is provided with a plurality of sealing rings. After the swab is used for sampling, the fixed ball at the top of the swab is embedded into the bottom of the mounting seat, then the swab is inserted into the sampling tube and is fully mixed with detection liquid in the sampling tube, the rubber plug is separated from the liquid outlet nozzle by rotating the sealing block, then the push rod is pushed, the detection liquid in the sampling tube is squeezed out, and detection is directly carried out; the detection liquid can be prevented from being in contact with air to cause pollution to the detection liquid; the symmetrically arranged rubber sheets can buffer the shell, the rubber sheets are arc-shaped, so that the buffering effect of the shell is better, and meanwhile, the spring arranged at the top of the base can buffer the shell again, so that the sampling tube can be prevented from being damaged when the sampling tube falls off.

The utility model relates to the technical field of HPV (human papillomavirus) detection devices, in particular to an HPV detection sample storage device which comprises a storage box and a storage mechanism, quick release structures are respectively arranged at the central positions of the inner walls of the front side and the rear side of the storage box, and the storage box is connected with the storage mechanism in a quick release manner through the two quick release structures. Clamping mechanisms are symmetrically arranged on the upper portions of the outer walls of the left side and the right side of the storage box correspondingly, limiting assemblies are symmetrically arranged on the lower portions of the outer walls of the left side and the right side of the storage box correspondingly, and during use, storage mechanisms containing sponge blocks of different sizes can be rapidly installed in the storage box through mutual cooperation of all structures in a rapid disassembly structure; and the stability of the storage mechanism mounted in the inner cavity of the storage box is ensured.

The invention provides an HPV (human papillomavirus) neutralizing antibody pseudovirus as well as a preparation method and application thereof, and belongs to a transgenic technology. The method comprises the following steps: firstly, constructing a 293TT cell strain capable of stably and highly expressing SV40 large T antigen, wherein the 293TT cell strain is used as an HPV (human papillomavirus) neutralizing antibody pseudovirus packaging tool cell; then fusing a 6X His tag at the N end or the C end of the L1 and L2 genes of the main capsid protein of the HPV virus to construct plasmids; the constructed plasmids and reporter gene plasmids jointly transfect 293TT cells, and after transfection, a Ni column is combined with a His tag for purification to obtain the neutralizing antibody pseudovirus. The pseudovirus is purified by using the Ni column, which is simple and efficient; the purified pseudovirus is high in purity, high in infection activity and complete in structure, and the particle size of the pseudovirus is consistent with that of the HPV live virus, and the structure is similar to that of the HPV live virus.

A microwave antenna device includes a conductive ground element defining a hole, a conductive elongated element extending through the hole and terminating at a distal end, and one or more dielectric elements. One or more of the dielectric elements electrically insulate the elongated element and the ground element from each other. The microwave antenna device can be configured to radiate microwave energy into surface tissue of the cervix to provide a therapeutic effect in one or more areas of the cervix (e.g., one or more areas of the cervix infected with human papillomavirus (HPV) and / or diagnosed with cervical intraepithelial neoplasia (CIN)), or to produce a correct biological response in one or more such areas. The microwave antenna device can be configured for local non-ablative thermal therapy of surface tissue of the cervix, local ablation of surface tissue of the cervix, and / or cauterization of surface tissue of the cervix.

The invention relates to the technical field of biomedicine, and discloses a method for constructing and culturing a normal cervical organ, which provides powerful technical support for exploring the pathogenesis of HPV (human papillomavirus) infected normal cervical epithelium.

Disclosed herein is an immunotherapy for human papillomavirus. More particular, disclosed is a therapeutic composition that includes one or more polypeptides that comprise a plurality of epitopes derived from multiple human papillomavirus antigens, which, when used in immunotherapy is capable of eliciting a protective and durable cell mediated immune response, without being limited thereto. Also disclosed are methods of treating or preventing HPV and / or cancer.

The invention provides a polypeptide and application thereof to treatment of HPV (human papillomavirus). Furthermore, the invention provides the effects of specifically inhibiting the HPV activity and promoting the apoptosis of HPV positive cells. After the polypeptide is used for treating an HPV (human papillomavirus) mouse model, the growth of tumors can be obviously inhibited, and the application prospect is relatively good.

This invention is directed to immunogenic composition, conjugates, virus-like particles (VLP) compositions, vaccines and methods directed to the treatment and / or prevent of infection by Human Papillomavirus.

The present invention provides a vaccine for preventing and / or treating infections with human papillomavirus. The present invention relates to a lipid particle encapsulating a nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus, wherein the lipid comprises a cationic lipid represented by general formula (Ia) or a pharmaceutically acceptable salt thereof:wherein R1 and R2 each independently represent a C1-C3 alkyl group;L1 represents a C17-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups;L2 represents a C10-C19 alkyl group which may have one or a plurality of C2-C4 alkanoyloxy groups or a C10-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups; andp is 3 or 4.

The present invention provides a primer-probe combination for detecting HPV and / or HPV typing, belonging to the field of human papillomavirus (HPV) detection technology. The primer-probe combination includes one or more of HPV16E7, HPV18E7, HPV31E7, HPV45E7, HPV33E7, HPV35E7, HPV59E6, HPV68E6, HPV58E7, HPV51E6, HPV52E7, HPV56E6, HPV66E6, and HPV39E6, and can distinguish different types of HPV, with good specificity, accuracy, and low detection limit. The present invention uses a digital PCR method (dPCR) to detect the fluorescent signal of each droplet, and calculates the copy number of the target gene fragment in the entire reaction system based on the Poisson distribution principle, so that absolute quantification can be achieved without relying on a standard curve.

The invention relates to the technical field of HPV (human papillomavirus) detection, and discloses an HPV rapid detection method based on an optimized CRISPR-Cas13a platform, which comprises the following steps: step S1, splitting and purifying a sample to obtain an HPV DNA (deoxyribonucleic acid) template; s2, performing amplification and transcription on the HPV target nucleic acid to obtain an amplification product; step S3, mixing an amplification product with a detection system of CRISPR-Cas13a protein, crRNA and reporter molecules, and then carrying out a cleavage reaction; and step S4, detecting the state change of the reporter molecule through a lateral flow chromatography test strip so as to judge whether the HPV exists in the sample or not. According to the invention, the recombinase polymerase is combined with CRISPR-Cas13a, so that the process from nucleic acid amplification to signal detection is completed at a single and mild constant temperature, the operation complexity and environmental requirements are remarkably reduced, and the HPV can be rapidly detected.

The invention relates to the related technical field of anti-HPV bioprotein gel production, in particular to an anti-HPV bioprotein gel preparation method and an anti-HPV bioprotein gel preparation device, the anti-HPV bioprotein gel preparation device comprises a preparation machine, and a fixing mechanism is arranged in the preparation machine. According to the preparation method and the preparation device of the anti-HPV biological protein gel, the fixing mechanism is arranged, and a sealing ring, a first fixing block, a nut, a second fixing block and a threaded rod are matched for use, so that a firmer fixing effect is realized, various problems caused by untight fixing are effectively prevented, and the cleaning mechanism is arranged, so that the anti-HPV biological protein gel can be conveniently cleaned. Through cooperative work of a water pump, a water inlet, a water outlet pipe, a fixing groove, an annular pipe and a nozzle, cleaning of the interior of the stirring tank is achieved, through a sealing mechanism, efficient sealing of the stirring tank is achieved through cooperative work of a first fixing plate, a limiting plate, a bolt, a first sealing plate, a second fixing plate, a sealing ring and a second sealing plate, and the sealing effect of the stirring tank is improved. The cleanliness and the safety of a preparation environment are greatly improved.

Provided are lipid particles encapsulating a nucleic acid capable of expressing an E6 antigen and an E7 antigen of human papillomavirus, whereby a vaccine for preventing and / or treating infection with human papillomavirus type 6 and / or type 11 can be provided. The lipid particles comprise a lipid that is a cationic lipid having the general formula (Ia), or a pharmaceutically acceptable salt thereof.[In the formula, R1, R2, p, L1 and L2 are as defined in the specification.]

The invention relates to the field of HPV (human papillomavirus) kits, in particular to an isothermal amplification rapid HPV detection kit which comprises a kit body, a kit cover, a refrigerator, a cooling cylinder and the like. A battery is arranged in the box body; the box body is detachably connected with a box cover; a plurality of refrigerators are fixedly connected in the box body; the box body is connected with cooling cylinders matched with the refrigerators in number. Through cooperation of a refrigerator, a cooling cylinder, a plug and a pushing system, only the refrigerator corresponding to the cooling cylinder filled with samples works, so that the problem that a large amount of energy is wasted due to the fact that an existing kit can only cool the whole interior and cannot independently cool test tubes filled with samples is solved, and meanwhile, the energy consumption is reduced. According to the kit, the prompt plate is matched with the pushing system, so that only the prompt plate corresponding to the cooling cylinder filled with the sample is exposed outside the kit body, and the problem that when an existing kit is used, a medical worker easily takes a test tube filled with the sample, namely the medical worker easily takes the test tube repeatedly is solved.