32 results about "Human papillomavirus" patented technology

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

The invention discloses an oral composition for preventing and treating HPV (human papillomavirus) based on enhancement of lower jiao immunity and application of the oral composition. The traditional Chinese medicine composition is prepared from, by weight, 1-3 parts of cinnamon, 3-10 parts of radix paeoniae rubra, 3-10 parts of peach kernels, 3-10 parts of cortex moutan, 3-10 parts of poria cocos, 5-15 parts of raw radix astragali, 1-3 parts of ginseng, 3-10 parts of rhizoma atractylodis macrocephalae, 1-3 parts of liquorice, 3-10 parts of rhizoma atractylodis, 3-10 parts of cortex phellodendri, 3-10 parts of radix cyathulae, 3-10 parts of semen coicis, 3-10 parts of thunberg fritillary bulb, 3-10 parts of honeysuckle, 3-10 parts of rhizoma smilacis glabrae, 3-10 parts of herba lycopi, 3-10 parts of herba leonuri and 3-10 parts of eucommia ulmoides. 3-10 parts of semen cuscutae, 3-10 parts of fennel, 3-10 parts of Chinese yam, 3-10 parts of radix linderae and 3-10 parts of rhizoma cyperi; through dual mechanisms of supporting healthy energy and eliminating evil and detoxifying, HPV viruses are effectively eliminated, the progress of lesion is blocked, and the traditional Chinese medicine composition has the advantages of various dosage forms, safety and mildness.

Methods for treating human papillomavirus (HPV) type 16- or HPV type 18-associated high-grade squamous intraepithelial lesions (HSIL) of the cervix are described

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

The present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine. In particular, the present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine, which is useful for topical application. The present invention also provides the use of such compositions for medical conditions in the keratinizing and non-keratinizing epithelium / skin, such as the treatment of human papillomavirus (HPV)-related pre-cancerous conditions.

Disclosed herein is an immunotherapy for human papillomavirus. More particular, disclosed is a therapeutic composition that includes one or more polypeptides that comprise a plurality of epitopes derived from multiple human papillomavirus antigens, which, when used in immunotherapy is capable of eliciting a protective and durable cell mediated immune response, without being limited thereto. Also disclosed are methods of treating or preventing HPV and / or cancer.

Provided are lipid particles encapsulating a nucleic acid capable of expressing an E6 antigen and an E7 antigen of human papillomavirus, whereby a vaccine for preventing and / or treating infection with human papillomavirus type 6 and / or type 11 can be provided. The lipid particles comprise a lipid that is a cationic lipid having the general formula (Ia), or a pharmaceutically acceptable salt thereof.[In the formula, R1, R2, p, L1 and L2 are as defined in the specification.]

The invention discloses a plant composite antibacterial liquid composition aiming at HPV (human papillomavirus) infection and a preparation method thereof, and belongs to the technical field of biological medicines. The composition is prepared from the following components in parts by weight: 5 to 20 parts of brucea javanica extract, 3 to 15 parts of senna extract, 2 to 10 parts of anhydridized beta-lactoglobulin, 1 to 8 parts of dandelion extract, 1 to 5 parts of penetration enhancer, 0.1 to 1 part of pH (Potential of Hydrogen) regulator, 0.1 to 0.5 part of preservative and the balance of phosphate buffer solution. According to the invention, anhydridized beta-lactoglobulin is innovatively taken as a carrier, so that targeted delivery of active ingredients is realized; the anti-HPV effect is enhanced through the synergistic effect of the brucea javanica and the kalii extract; the anti-inflammatory repair function of the dandelion extract is combined, and a compound penetration enhancer and a specific preparation process are matched, so that the product has the characteristics of efficient HPV resistance, anti-inflammatory repair, mildness, no irritation and good stability.

This invention relates to antisense oligonucleotides targeting different subtypes of human papillomavirus (HPV) oncogenes and their applications. The ASO molecules are 14–40 bases in length and specifically pair with specific regions of target genes. This invention also relates to the following applications of the ASO molecules: (1) preparing preparations to inhibit HPV virus expression; or (2) preparing drugs or drug compositions for treating diseases related to HPV infection; or (3) treating diseases related to HPV infection; or (4) inhibiting the expression of HPV virus genes.

The invention relates to a recombinant bioactive protein for preventing or treating human papilloma virus infection as well as a preparation method and application thereof, and belongs to the technical field of medicines. The recombinant bioactive protein disclosed by the invention is anhydridized recombinant lactoferrin, anhydridized recombinant lactoglobulin or anhydridized recombinant histone; the recombinant bioactive protein is obtained by carrying out anhydridation modification on recombinant lactoferrin, recombinant lactoglobulin or recombinant histone by using 3-hydroxyphthalic anhydride or a derivative thereof, 4-hydroxyphthalic anhydride or a derivative thereof, succinic anhydride or maleic anhydride. The bioactive protein disclosed by the invention has the advantages of preventing HPV virus from invading cells, preventing virus from expanding infection, resisting bacteria and oxidation, being safe, stable, convenient to store and transport, low in cost and the like.

This invention provides a non-classical crRNA, reagent, kit, and application for one-step RPA-Cas12a nucleic acid detection. Based on classic crRNA, the non-classical crRNA shortens the 5' end by two bases and / or adjusts the stem-loop base structure to one of UCUUUU, UCUUU, UGUUU, UGUU, or UAAGU. This non-classical crRNA structure reduces the affinity of the Cas12a-crRNA complex for the target DNA, weakens the cis-cleavage activity of Cas12a, and increases the trans-cleavage activity of Cas12a, without requiring additional base modifications. This invention achieves a detection limit of 1 fM in the detection of Group B Streptococcus and Human Papillomavirus 16, with a sensitivity approximately 10-fold higher than before optimization.

The present invention relates to a pharmaceutical composition comprising an immunoglobulin Fc region and an IL-7 fusion protein. Specifically, when a fusion protein comprising the immunoglobulin Fc region and IL-7 is administered to an affected area, a strong immune response is induced in the body and thus allows human papillomavirus-caused diseases to be prevented or treated.

The invention relates to traditional Chinese medicine external gel for preventing or treating HPV (human papillomavirus) infection as well as a preparation method and application of the traditional Chinese medicine external gel. The traditional Chinese medicine external gel comprises an active composition and a gel matrix, the active composition comprises a traditional Chinese medicine extract and menthol, and the traditional Chinese medicine extract comprises the following raw materials in parts by weight: 9-15 parts of radix sophorae flavescentis, 9-15 parts of cortex phellodendri, 12-20 parts of rhizoma smilacis glabrae, 9-12 parts of honeysuckle, 9-12 parts of fructus forsythiae, 6-12 parts of safflower, 12-20 parts of fructus cnidii, 6-12 parts of rhizoma bletillae, 6-12 parts of radix aucklandiae and 12-20 parts of cockscomb; 5 to 10 parts by weight of menthol; the weight part of the gel matrix is 260 to 800 parts. The traditional Chinese medicine external gel provided by the invention can effectively inhibit HPV virus replication, treat cervical precancerous lesions caused by HPV infection and prevent cancers caused by HPV infection, and the traditional Chinese medicine external gel is convenient to use, stable in action effect and high in safety, and has important clinical significance.

The application discloses an application of a His-SUMO tag in preparation of a recombinant HPV18 E7 protein and belongs to the technical field of recombinant protein expression. The application is high-efficiency expression of a soluble HPV18 E7 fusion protein (His-SUMO-HPV18 E7) induced by a His+SUMO tag, and preparation of a recombinant HPV18 E7 protein (No tag HPV18 E7) without redundant tag amino acid sequences by affinity chromatography purification and tag removal technology, so as to solve the problems of low soluble expression amount and / or large molecular tag in the prior art. The prepared recombinant human papillomavirus HPV18 E7 protein has clear and single SDS-PAGE electrophoresis bands, good repeatability, high yield (up to 20.5 mg / L), and a protein purity of up to 99%, and can be effectively applied to large-scale production of the recombinant human papillomavirus HPV18 E7 protein.

The invention discloses a purification device with an HPV inactivation effect for hawthorn kernel tar lotion production, and relates to the technical field of grease production, the purification device comprises a rack, a discharging frame is arranged on one side of the rack, a feeding frame is arranged on the other side of the rack, a rotating cylinder is rotatably connected in the rack, and a rotating shaft is arranged in the rotating cylinder. The two ends of the rotating cylinder communicate with the discharging frame and the feeding frame correspondingly. According to the invention, the first heating rod is used as an external heat source, and the second heating rods are circumferentially arranged at the center, so that a bidirectional synergistic heat radiation pattern from outside to inside and from inside to outside is formed, and the inherent defects of edge overheating carbonization and insufficient center pyrolysis of a traditional external heating type retort are effectively overcome. The hawthorn seed material is heated more uniformly, lignocellulose is cracked more sufficiently, the yield of light phenol components such as guaiacol with an HPV inactivation effect is remarkably improved, the consistency of batch-to-batch m-distillate components is enhanced, and the strict requirement of medicinal lotion for effect stability is met.

The utility model discloses a portable HPV (human papillomavirus) detection rod which comprises a contraction shell, a telescopic rod body used for enabling a sampled sample to contract into the contraction shell for positioning is arranged in the contraction shell in a contraction mode, and a detection head enabling the telescopic rod body to be pushed in a reciprocating mode is arranged at one end of the telescopic rod body. A telescopic rod body slides in a strip-shaped positioning groove in a strip-shaped positioning part in a positioning manner by utilizing a pushing block, so that a protection plate piece and a detection head extend out of one end of a telescopic shell, and can extend into the cervix uteri to obtain a sample, and after the sample is obtained, the telescopic rod body is quickly retracted into the telescopic shell to be positioned and kept stable by utilizing retraction of the telescopic rod body; the position of the detection head in the telescopic shell is kept stable, the problem that the detection head touches the inner wall and a port in the storage process is avoided, meanwhile, the detection head can be precisely retracted into the storage shell for protection after sampling, and the safety of a detection sample is further improved.

The present invention provides compositions and methods for treating HPV infections, including pre-malignant and cancerous infections. Compounds that specifically bind to and inactivate the HPV E6 protein are disclosed.

The invention relates to application of a vaccinia virus inflammatory rabbit skin extract in preparation of an HPV (human papillomavirus) inhibitor.

Provided herein are varicella-zoster virus vaccine, human papillomavirus vaccine, and Rabies vaccine antigens, compositions thereof, and uses thereof in cancer immunotherapy and cancer treatment.

This invention provides a mutant human papillomavirus (HPV) type 16 E2 protein and its application. The mutant E2 protein is obtained by the following three mutations based on the wild-type HPV type 16 E2 protein: (1) the serine at position 317 of the wild-type HPV type 16 E2 protein is mutated to proline; (2) the threonine at position 17 of the wild-type HPV type 16 E2 protein is mutated to arginine, and the asparagine at position 55 is mutated to arginine; (3) the histidine at position 218 of the wild-type HPV type 16 E2 protein is mutated to proline. This invention obtains the target protein through amino acid mutation and codon optimization, resulting in a more stable protein structure that is more conducive to expression, thereby significantly improving the yield and preparation efficiency of the HPV type 16 E2 protein. This antigen can be used for the detection of HPV type 16 E2 antibodies or to evaluate the humoral immunogenicity of therapeutic vaccines targeting HPV type 16 E2.

The invention relates to the technical field of medical treatment and public health disinfection, and relates to a pH-sensitive medical wet tissue for preventing or treating human papilloma virus and a preparation method and application thereof, the pH-sensitive medical wet tissue comprises the following raw materials by weight: 0.1-3 parts of an inorganic antibacterial material, 0.1-1 part of an antibacterial peptide material, 0.1-1 part of a quaternary ammonium salt antibacterial material, 0.1-0.5 part of a gel and 0.1-0.5 part of a pH regulator. In the formula, the antibacterial peptide material is combined with antiviral components of the inorganic antibacterial material and the quaternary ammonium salt antibacterial material, and the antibacterial effect of the antibacterial peptide material and the quaternary ammonium salt antibacterial material can be enhanced due to the antibacterial property of the inorganic antibacterial material; the damage effect of the quaternary ammonium salt antibacterial material on viruses also helps the antibacterial peptide material and the inorganic antibacterial material to better deal with possible virus-bacteria mixed pollution, the three components have a synergistic effect, HPV viruses and germs can be synergistically and efficiently killed, and the safety is improved while the cost is reduced.

The invention belongs to the technical field of HPV (human papillomavirus) detection, and particularly relates to a primer probe group and a kit for specific recognition and rapid detection of fourteen high-risk HPVs based on an RPA (recombinase polymerase amplification) isothermal amplification method, and application of the primer probe group and the kit. The primer probe group comprises a primer group and a probe group, wherein the primer group comprises a peripheral upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1, a peripheral downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a peripheral downstream primer with a nucleotide sequence as shown in SEQ ID NO: 3; the nucleotide sequence of the peripheral downstream primer is as shown in SEQ ID NO: 2. The primer probe set and the kit have high sensitivity and high specificity, the detection sensitivity of the primer probe set and the kit to HPV high-risk viruses can reach 100 copies / mL, and the performance is superior to that of existing detection products in the market; the design of the specific probe can accurately detect fourteen high-risk HPV types, and does not generate cross reaction with low-risk HPV types or other viruses. The whole detection process is carried out at room temperature, laboratory operation is not needed, the detection time is about 30 minutes, and the detection can be completed in one detection rod.

In one aspect, the disclosure relates to proteolysis targeting chimeras (PROTACs) targeting SMYD3, methods of making the same, pharmaceutical compositions comprising same, and methods of treating squamous cell carcinomas (SCC) and other cancers using the same. In some aspects, the cancers are human papillomavirus (HPV) negative. In another aspect, disclosed are new SMYD3 inhibitors. Also disclosed are methods of killing cancer cells.

The invention is a composition for the treatment of HPV infections, which, with its components containing [3-caryophyllene, geranyl acetate, geraniol, and nerol, modulates the E7 and E2 protein pathways to provide a natural supportive therapeutic effect in managing HPV infections, exhibits antiviral, antibacterial, and immunomodulatory activity, significantly reduces viral replication, and suppresses protein expression, Contains Eugenia aromaticum (clove oil), which prevents viral replication by disrupting the structural integrity of cell membranes, exhibiting antimicrobial and antiviral activity with its high eugenol content, and Contains Cymbopogon martinii (palmarosa oil), which disrupts viral cell walls and inhibits the replication process, exhibiting antiviral and antibacterial properties with its high geraniol and citronellal content, and Contains Melaleuca viridiflora (niaouli oil), which strengthens cellular immune mechanisms to limit the intracellular spread of viruses and exhibits antiviral and immunomodulatory effects with its viridiflorol content.

The invention belongs to the technical field of biological medicine, and particularly discloses an extracellular vesicle and application thereof in prevention and treatment of HPV infection. The preparation method comprises the following steps: carrying out co-fermentation on composite probiotics, collecting a fermentation supernatant, and carrying out ultrafiltration to obtain a composite probiotic fermentation active substance; adding into a mesenchymal stem cell complete culture medium to prepare a treatment culture medium; culturing the mesenchymal stem cells by using a treatment culture medium, collecting a culture solution, centrifuging, and collecting supernate; and sequentially carrying out differential centrifugation, filtering with a 0.22 mu m filtering membrane, and purifying to obtain the extracellular vesicles. The extracellular vesicle loaded antiviral component derived from mesenchymal stem cells has excellent biocompatibility, low immunogenicity and targeting property, can effectively neutralize HPV virus and inhibit proliferation of infected cells, and provides a novel stem cell derived treatment strategy for prevention and treatment of HPV.

The invention discloses application of dextran sulfate sodium in preparation of a medicine for treating and preventing human papilloma virus infection. According to the application, the action effect of the dextran sulfate on inhibiting human papilloma virus type 6, type 16, type 18 and type 58 pseudoviruses from entering 293FT cells is evaluated at the cellular level, and the result shows that the dextran sulfate can effectively inhibit the human papilloma virus type 6, type 16, type 18 and type 58 from infecting the 293FT cells. Results of inhibition of human papilloma virus 16 pseudovirus infection by sodium dextran sulfate according to animal level evaluation show that sodium dextran sulfate can effectively inhibit infection of human papilloma virus 16 pseudovirus on mouse genitals. Sodium dextran sulfate has the advantages of blocking HPV virus from invading cells, preventing virus from expanding infection, being safe, stable, low in cost and the like, and the medicine has the prospect of being developed into a preparation for treating or preventing human papilloma virus infection related diseases.

The invention discloses a preparation method of a detoxification and leucorrhea arresting mixture, the mixture prepared by the method, and a quality control method and application of the mixture. The mixture is characterized in that in every 1mL of the mixture, the content of psoralen (C11H6O3) is not less than 14.89 [mu] g, and the content of astilbin (C21H22O11) is not less than 0.54 mg. The preparation method comprises the integrated steps of extracting medicinal materials, concentrating an extracting solution until the relative density is 1.06-1.10 at the temperature of 60 DEG C, standing and purifying, and adding stevioside and sodium benzoate in a specific proportion to prepare liquid and form. The invention also establishes a comprehensive quality control system covering thin-layer chromatography identification of multiple medicines including ovateleaf holly bark, pericarpium citri reticulatae, liquorice and plantain herb and content determination of the two-index components. In-vitro and clinical evidences show that the mixture has antibacterial and anti-inflammatory effects and has a good application prospect in treating gynecological diseases related to human papilloma virus (HPV) infection.

Multiantigenic human papillomavirus (HPV) molecular vaccine constructs for use and treatment of HPV-related disorders and conditions, e.g., HPV molecular vaccines targeting HPV16, HPV18, and HPV-45-related conditions.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a kit and a detection method for normal-temperature nucleic acid detection of HPV (human papillomavirus). The detection reagent is a freeze-drying reagent prepared from an RPA / CRISPR detection system, and the detection system comprises an RPA isothermal amplification reagent, a Buffer A, a front primer, a rear primer, Cas12a, gRNA, ssDNA reporter molecules, a Buffer 2.1 and a mixed freeze-drying protective additive. The composition of the mixed freeze-drying protective agent is optimized according to the composition of a detection system and a specific detection method. According to the invention, the problems of normal-temperature storage and transportation of the one-stop RPA / CRISPR detection reagent of the HPV virus are solved, the detection performance is ensured, an efficient and accurate solution is provided for large-scale screening, and the kit has a very good application prospect.