40 results about "Human papillomavirus" patented technology

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

The invention discloses an oral composition for preventing and treating HPV (human papillomavirus) based on enhancement of lower jiao immunity and application of the oral composition. The traditional Chinese medicine composition is prepared from, by weight, 1-3 parts of cinnamon, 3-10 parts of radix paeoniae rubra, 3-10 parts of peach kernels, 3-10 parts of cortex moutan, 3-10 parts of poria cocos, 5-15 parts of raw radix astragali, 1-3 parts of ginseng, 3-10 parts of rhizoma atractylodis macrocephalae, 1-3 parts of liquorice, 3-10 parts of rhizoma atractylodis, 3-10 parts of cortex phellodendri, 3-10 parts of radix cyathulae, 3-10 parts of semen coicis, 3-10 parts of thunberg fritillary bulb, 3-10 parts of honeysuckle, 3-10 parts of rhizoma smilacis glabrae, 3-10 parts of herba lycopi, 3-10 parts of herba leonuri and 3-10 parts of eucommia ulmoides. 3-10 parts of semen cuscutae, 3-10 parts of fennel, 3-10 parts of Chinese yam, 3-10 parts of radix linderae and 3-10 parts of rhizoma cyperi; through dual mechanisms of supporting healthy energy and eliminating evil and detoxifying, HPV viruses are effectively eliminated, the progress of lesion is blocked, and the traditional Chinese medicine composition has the advantages of various dosage forms, safety and mildness.

Methods for treating human papillomavirus (HPV) type 16- or HPV type 18-associated high-grade squamous intraepithelial lesions (HSIL) of the cervix are described

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

The utility model relates to the technical field of hypochlorous acid gel production, in particular to a stirring mechanism for anti-HPV (human papillomavirus) carbomer hypochlorous acid gel production, which comprises a mixing box, a top plate, a feeding pipe, a discharging pipe and a staged quantitative stirring component, the staged quantitative stirring component comprises an object placing plate, a weighing column, a spring and a storage box, the top plate is detachably connected with the mixing box, and the top plate is detachably connected with the storage box. The feeding pipe is detachably connected with the top plate and located on the upper portion of the interior of the top plate, the discharging pipe is detachably connected with the mixing box, the storage plate is fixedly connected with the feeding pipe, the weighing column is detachably connected with the storage plate, the spring is arranged on the outer surface of the weighing column, and the storage box is detachably connected with the weighing column. Therefore, on the premise that the stirring and mixing quality is guaranteed, the problems that when carbomer hypochlorous acid gel is produced, graded input cannot be carried out, various materials are prone to being left in a pipeline of the feeding port at the same time, and the production quality is affected are effectively solved.

The present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine. In particular, the present invention provides a composition comprising a cytidine analog, in particular decitabine or azacitidine, which is useful for topical application. The present invention also provides the use of such compositions for medical conditions in the keratinizing and non-keratinizing epithelium / skin, such as the treatment of human papillomavirus (HPV)-related pre-cancerous conditions.

Disclosed herein is an immunotherapy for human papillomavirus. More particular, disclosed is a therapeutic composition that includes one or more polypeptides that comprise a plurality of epitopes derived from multiple human papillomavirus antigens, which, when used in immunotherapy is capable of eliciting a protective and durable cell mediated immune response, without being limited thereto. Also disclosed are methods of treating or preventing HPV and / or cancer.

The present invention provides a primer-probe combination for detecting HPV and / or HPV typing, belonging to the field of human papillomavirus (HPV) detection technology. The primer-probe combination includes one or more of HPV16E7, HPV18E7, HPV31E7, HPV45E7, HPV33E7, HPV35E7, HPV59E6, HPV68E6, HPV58E7, HPV51E6, HPV52E7, HPV56E6, HPV66E6, and HPV39E6, and can distinguish different types of HPV, with good specificity, accuracy, and low detection limit. The present invention uses a digital PCR method (dPCR) to detect the fluorescent signal of each droplet, and calculates the copy number of the target gene fragment in the entire reaction system based on the Poisson distribution principle, so that absolute quantification can be achieved without relying on a standard curve.

The invention relates to the technical field of HPV (human papillomavirus) detection, and discloses an HPV rapid detection method based on an optimized CRISPR-Cas13a platform, which comprises the following steps: step S1, splitting and purifying a sample to obtain an HPV DNA (deoxyribonucleic acid) template; s2, performing amplification and transcription on the HPV target nucleic acid to obtain an amplification product; step S3, mixing an amplification product with a detection system of CRISPR-Cas13a protein, crRNA and reporter molecules, and then carrying out a cleavage reaction; and step S4, detecting the state change of the reporter molecule through a lateral flow chromatography test strip so as to judge whether the HPV exists in the sample or not. According to the invention, the recombinase polymerase is combined with CRISPR-Cas13a, so that the process from nucleic acid amplification to signal detection is completed at a single and mild constant temperature, the operation complexity and environmental requirements are remarkably reduced, and the HPV can be rapidly detected.

The invention relates to the related technical field of anti-HPV bioprotein gel production, in particular to an anti-HPV bioprotein gel preparation method and an anti-HPV bioprotein gel preparation device, the anti-HPV bioprotein gel preparation device comprises a preparation machine, and a fixing mechanism is arranged in the preparation machine. According to the preparation method and the preparation device of the anti-HPV biological protein gel, the fixing mechanism is arranged, and a sealing ring, a first fixing block, a nut, a second fixing block and a threaded rod are matched for use, so that a firmer fixing effect is realized, various problems caused by untight fixing are effectively prevented, and the cleaning mechanism is arranged, so that the anti-HPV biological protein gel can be conveniently cleaned. Through cooperative work of a water pump, a water inlet, a water outlet pipe, a fixing groove, an annular pipe and a nozzle, cleaning of the interior of the stirring tank is achieved, through a sealing mechanism, efficient sealing of the stirring tank is achieved through cooperative work of a first fixing plate, a limiting plate, a bolt, a first sealing plate, a second fixing plate, a sealing ring and a second sealing plate, and the sealing effect of the stirring tank is improved. The cleanliness and the safety of a preparation environment are greatly improved.

Provided are lipid particles encapsulating a nucleic acid capable of expressing an E6 antigen and an E7 antigen of human papillomavirus, whereby a vaccine for preventing and / or treating infection with human papillomavirus type 6 and / or type 11 can be provided. The lipid particles comprise a lipid that is a cationic lipid having the general formula (Ia), or a pharmaceutically acceptable salt thereof.[In the formula, R1, R2, p, L1 and L2 are as defined in the specification.]

The invention discloses a plant composite antibacterial liquid composition aiming at HPV (human papillomavirus) infection and a preparation method thereof, and belongs to the technical field of biological medicines. The composition is prepared from the following components in parts by weight: 5 to 20 parts of brucea javanica extract, 3 to 15 parts of senna extract, 2 to 10 parts of anhydridized beta-lactoglobulin, 1 to 8 parts of dandelion extract, 1 to 5 parts of penetration enhancer, 0.1 to 1 part of pH (Potential of Hydrogen) regulator, 0.1 to 0.5 part of preservative and the balance of phosphate buffer solution. According to the invention, anhydridized beta-lactoglobulin is innovatively taken as a carrier, so that targeted delivery of active ingredients is realized; the anti-HPV effect is enhanced through the synergistic effect of the brucea javanica and the kalii extract; the anti-inflammatory repair function of the dandelion extract is combined, and a compound penetration enhancer and a specific preparation process are matched, so that the product has the characteristics of efficient HPV resistance, anti-inflammatory repair, mildness, no irritation and good stability.

The present invention relates to a pharmaceutical composition comprising an immunoglobulin Fc region and an IL-7 fusion protein. Specifically, when a fusion protein comprising the immunoglobulin Fc region and IL-7 is administered to an affected area, a strong immune response is induced in the body and thus allows human papillomavirus-caused diseases to be prevented or treated.

This invention relates to antisense oligonucleotides targeting different subtypes of human papillomavirus (HPV) oncogenes and their applications. The ASO molecules are 14–40 bases in length and specifically pair with specific regions of target genes. This invention also relates to the following applications of the ASO molecules: (1) preparing preparations to inhibit HPV virus expression; or (2) preparing drugs or drug compositions for treating diseases related to HPV infection; or (3) treating diseases related to HPV infection; or (4) inhibiting the expression of HPV virus genes.

The utility model discloses a sampling test tube for human papilloma virus inhibitor detection and analysis, which comprises a test tube body, the outer surface of the test tube body is fixedly connected with a connecting table at the upper edge position, the bottom end of the connecting table is in threaded connection with a protective jacket, and the outer surface of the protective jacket is fixedly communicated with an exhaust tube. And the bottom end of the exhaust pipe fixedly communicates with an adjusting pipe. Through the arrangement of the protective jacket, the test tube body can be effectively protected, so that the test tube body can be effectively prevented from being directly damaged when collision occurs, the service life is prolonged, and furthermore, a vacuum pump can be connected with an exhaust pipe to extract air between the test tube body and the protective jacket to form a vacuum environment; a sample in the test tube body is difficult to be interfered by external air temperature, and the quality of the sample is improved.

The invention relates to a recombinant bioactive protein for preventing or treating human papilloma virus infection as well as a preparation method and application thereof, and belongs to the technical field of medicines. The recombinant bioactive protein disclosed by the invention is anhydridized recombinant lactoferrin, anhydridized recombinant lactoglobulin or anhydridized recombinant histone; the recombinant bioactive protein is obtained by carrying out anhydridation modification on recombinant lactoferrin, recombinant lactoglobulin or recombinant histone by using 3-hydroxyphthalic anhydride or a derivative thereof, 4-hydroxyphthalic anhydride or a derivative thereof, succinic anhydride or maleic anhydride. The bioactive protein disclosed by the invention has the advantages of preventing HPV virus from invading cells, preventing virus from expanding infection, resisting bacteria and oxidation, being safe, stable, convenient to store and transport, low in cost and the like.

This invention provides a non-classical crRNA, reagent, kit, and application for one-step RPA-Cas12a nucleic acid detection. Based on classic crRNA, the non-classical crRNA shortens the 5' end by two bases and / or adjusts the stem-loop base structure to one of UCUUUU, UCUUU, UGUUU, UGUU, or UAAGU. This non-classical crRNA structure reduces the affinity of the Cas12a-crRNA complex for the target DNA, weakens the cis-cleavage activity of Cas12a, and increases the trans-cleavage activity of Cas12a, without requiring additional base modifications. This invention achieves a detection limit of 1 fM in the detection of Group B Streptococcus and Human Papillomavirus 16, with a sensitivity approximately 10-fold higher than before optimization.

The invention discloses a cervix sampler, an operation method thereof and a kit, the sampler comprises a shell, the shell comprises a shell side wall and a closed shell far-end wall, the shell side wall comprises a first notch, and the first notch is arranged close to the shell far-end wall; the sampling brush is configured to be accommodated in the shell, and at least one part of the sampling brush is exposed to the position of the first notch in at least one use state; the shaft comprises a shaft far end and a shaft near end, the shaft far end is connected with the sampling brush, the shaft near end further comprises an operator, and the operator drives the shaft and the sampling brush to rotate in the circumferential direction under the operation of external force, so that the sampling brush makes contact with a target sample near the first notch in the using state, and friction force is generated to obtain biological tissue. The sampler can be conveniently placed at a target position and sample tissue can be conveniently obtained. The collected biological tissues can be cervical cells, and can be used for double detection (PAP / HPV Co-Testing) of cervical smear (PAP) and human papilloma virus (HPV), and can be used for screening cervical cancer.

The present invention relates to a pharmaceutical composition comprising an immunoglobulin Fc region and an IL-7 fusion protein. Specifically, when a fusion protein comprising the immunoglobulin Fc region and IL-7 is administered to an affected area, a strong immune response is induced in the body and thus allows human papillomavirus-caused diseases to be prevented or treated.

The invention relates to traditional Chinese medicine external gel for preventing or treating HPV (human papillomavirus) infection as well as a preparation method and application of the traditional Chinese medicine external gel. The traditional Chinese medicine external gel comprises an active composition and a gel matrix, the active composition comprises a traditional Chinese medicine extract and menthol, and the traditional Chinese medicine extract comprises the following raw materials in parts by weight: 9-15 parts of radix sophorae flavescentis, 9-15 parts of cortex phellodendri, 12-20 parts of rhizoma smilacis glabrae, 9-12 parts of honeysuckle, 9-12 parts of fructus forsythiae, 6-12 parts of safflower, 12-20 parts of fructus cnidii, 6-12 parts of rhizoma bletillae, 6-12 parts of radix aucklandiae and 12-20 parts of cockscomb; 5 to 10 parts by weight of menthol; the weight part of the gel matrix is 260 to 800 parts. The traditional Chinese medicine external gel provided by the invention can effectively inhibit HPV virus replication, treat cervical precancerous lesions caused by HPV infection and prevent cancers caused by HPV infection, and the traditional Chinese medicine external gel is convenient to use, stable in action effect and high in safety, and has important clinical significance.

The application discloses an application of a His-SUMO tag in preparation of a recombinant HPV18 E7 protein and belongs to the technical field of recombinant protein expression. The application is high-efficiency expression of a soluble HPV18 E7 fusion protein (His-SUMO-HPV18 E7) induced by a His+SUMO tag, and preparation of a recombinant HPV18 E7 protein (No tag HPV18 E7) without redundant tag amino acid sequences by affinity chromatography purification and tag removal technology, so as to solve the problems of low soluble expression amount and / or large molecular tag in the prior art. The prepared recombinant human papillomavirus HPV18 E7 protein has clear and single SDS-PAGE electrophoresis bands, good repeatability, high yield (up to 20.5 mg / L), and a protein purity of up to 99%, and can be effectively applied to large-scale production of the recombinant human papillomavirus HPV18 E7 protein.

The invention discloses a purification device with an HPV inactivation effect for hawthorn kernel tar lotion production, and relates to the technical field of grease production, the purification device comprises a rack, a discharging frame is arranged on one side of the rack, a feeding frame is arranged on the other side of the rack, a rotating cylinder is rotatably connected in the rack, and a rotating shaft is arranged in the rotating cylinder. The two ends of the rotating cylinder communicate with the discharging frame and the feeding frame correspondingly. According to the invention, the first heating rod is used as an external heat source, and the second heating rods are circumferentially arranged at the center, so that a bidirectional synergistic heat radiation pattern from outside to inside and from inside to outside is formed, and the inherent defects of edge overheating carbonization and insufficient center pyrolysis of a traditional external heating type retort are effectively overcome. The hawthorn seed material is heated more uniformly, lignocellulose is cracked more sufficiently, the yield of light phenol components such as guaiacol with an HPV inactivation effect is remarkably improved, the consistency of batch-to-batch m-distillate components is enhanced, and the strict requirement of medicinal lotion for effect stability is met.

The utility model discloses a portable HPV (human papillomavirus) detection rod which comprises a contraction shell, a telescopic rod body used for enabling a sampled sample to contract into the contraction shell for positioning is arranged in the contraction shell in a contraction mode, and a detection head enabling the telescopic rod body to be pushed in a reciprocating mode is arranged at one end of the telescopic rod body. A telescopic rod body slides in a strip-shaped positioning groove in a strip-shaped positioning part in a positioning manner by utilizing a pushing block, so that a protection plate piece and a detection head extend out of one end of a telescopic shell, and can extend into the cervix uteri to obtain a sample, and after the sample is obtained, the telescopic rod body is quickly retracted into the telescopic shell to be positioned and kept stable by utilizing retraction of the telescopic rod body; the position of the detection head in the telescopic shell is kept stable, the problem that the detection head touches the inner wall and a port in the storage process is avoided, meanwhile, the detection head can be precisely retracted into the storage shell for protection after sampling, and the safety of a detection sample is further improved.

The present invention provides compositions and methods for treating HPV infections, including pre-malignant and cancerous infections. Compounds that specifically bind to and inactivate the HPV E6 protein are disclosed.

Compositions and methods for treating a viral condition. The composition is administered to the subject in a therapeutic amount. The composition includes a first medicament effective for up-regulating disulfide bond donation and exchange, and a second medicament comprising a DNA polymerase inhibitor, a protease inhibitor, a DNA / RNA inhibitor, an intron splicer, or combinations thereof. The first medicament is effective for causing viruses to dissociate from integration sites and actively replicate with availability of disulfide bonds. The second medicament is effective for supporting or improving degradation, slicing, and / or inhibition of viral RNA, viral DNA, and / or viral proteins. The virus is Coronavirus, Epstein Barr virus, human papillomavirus, or herpes simplex virus.

The invention relates to application of a vaccinia virus inflammatory rabbit skin extract in preparation of an HPV (human papillomavirus) inhibitor.

Provided herein are varicella-zoster virus vaccine, human papillomavirus vaccine, and Rabies vaccine antigens, compositions thereof, and uses thereof in cancer immunotherapy and cancer treatment.

This invention provides a mutant human papillomavirus (HPV) type 16 E2 protein and its application. The mutant E2 protein is obtained by the following three mutations based on the wild-type HPV type 16 E2 protein: (1) the serine at position 317 of the wild-type HPV type 16 E2 protein is mutated to proline; (2) the threonine at position 17 of the wild-type HPV type 16 E2 protein is mutated to arginine, and the asparagine at position 55 is mutated to arginine; (3) the histidine at position 218 of the wild-type HPV type 16 E2 protein is mutated to proline. This invention obtains the target protein through amino acid mutation and codon optimization, resulting in a more stable protein structure that is more conducive to expression, thereby significantly improving the yield and preparation efficiency of the HPV type 16 E2 protein. This antigen can be used for the detection of HPV type 16 E2 antibodies or to evaluate the humoral immunogenicity of therapeutic vaccines targeting HPV type 16 E2.

The invention relates to the technical field of medical treatment and public health disinfection, and relates to a pH-sensitive medical wet tissue for preventing or treating human papilloma virus and a preparation method and application thereof, the pH-sensitive medical wet tissue comprises the following raw materials by weight: 0.1-3 parts of an inorganic antibacterial material, 0.1-1 part of an antibacterial peptide material, 0.1-1 part of a quaternary ammonium salt antibacterial material, 0.1-0.5 part of a gel and 0.1-0.5 part of a pH regulator. In the formula, the antibacterial peptide material is combined with antiviral components of the inorganic antibacterial material and the quaternary ammonium salt antibacterial material, and the antibacterial effect of the antibacterial peptide material and the quaternary ammonium salt antibacterial material can be enhanced due to the antibacterial property of the inorganic antibacterial material; the damage effect of the quaternary ammonium salt antibacterial material on viruses also helps the antibacterial peptide material and the inorganic antibacterial material to better deal with possible virus-bacteria mixed pollution, the three components have a synergistic effect, HPV viruses and germs can be synergistically and efficiently killed, and the safety is improved while the cost is reduced.