290 results about "Precancerous lesion" patented technology

A non-invasive tool for skin disease diagnosis would be a useful clinical adjunct. The purpose of this study was to determine whether visible / near-infrared spectroscopy can be used to non-invasively characterize skin diseases. In-vivo visible- and near-infrared spectra (400-2500 nm) of skin neoplasms (actinic keratoses, basal cell carcinomata, banal common acquired melanocytic nevi, dysplastic melanocytic nevi, actinic lentigines and seborrheic keratoses) were collected by placing a fiber optic probe on the skin. Paired t-tests, repeated measures analysis of variance and linear discriminant analysis were used to determine whether significant spectral differences existed and whether spectra could be classified according to lesion type. Paired t-tests showed significant differences (p<0.05) between normal skin and skin lesions in several areas of the visible / near-infrared spectrum. In addition, significant differences were found between the lesion groups by analysis of variance. Linear discriminant analysis classified spectra from benign lesions compared to pre-malignant or malignant lesions with high accuracy. Visible / near-infrared spectroscopy is a promising non-invasive technique for the screening of skin diseases.

The invention belongs to the fields of gene engineering and oncology, and discloses a serum / plasma miRNA serum marker related to cervical carcinoma and precancerous lesions thereof and application thereof. The marker is single miR-21 or the combination of miR-21 and miR-29a. The marker and the primer thereof can be used for preparing diagnosis kits which are used for assistant early diagnosis of cervical carcinoma and precancerous lesions thereof.

The invention discloses a colposcopy image-based cervical cancer detection method, device and terminal equipment and a computer readable storage medium. The method comprises the following steps of: ina bidirectional convolutional neural network-based cervical cancer detection model, positioning and extracting a cervical opening position of an obtained colposcopy image so as to generate an ROI image comprising a cervical cancer disease area; extracting a cutting edge of the ROI image through a bidirectional convolutional neural network so as to generate a cut image; and carrying out cancer grade classification on the cut image through a classification convolutional neural network so as to output a cervical cancer lesion grade and then rapidly and correctly obtain a cervical cancer detection result. According to the method, doctors that are lack of experiences can be helped to rapidly judge diseased regions, discover atypical diseased regions and judge the disease degrees and sampling regions, so that help and auxo-action are provided for discovering cervical cancer and precancerous lesions.

The invention relates to a sieving and checking method for cancers, comprising the following steps: (1), providing a test body to be checked; (2), checking the CpG sequence methylation state of at least one target gene in the genome DNA of the test body to be checked, wherein at least one of the target genes comprises SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1; and (3), judging whether the test body has cancers or precancerous lesions or not according to the existence or inexistence of the methylation state of the target genes. The methylation state checking method comprises a methylation specific polymerase chain reaction, a quantitative methylation specific polymerase chain reaction, sulphite sequencing, micro-arrays, mass spectrograph analysis or denaturation high-efficiency liquid chromatography, and the like.

The invention discloses a high-throughput multi-index protein chip for screening esophageal squamous carcinoma specific protein markers, and discloses a kit supporting the use. 55 types of esophageal squamous carcinoma differential proteins in the serum or blood plasma are selected and used as the esophageal squamous carcinoma protein markers and protein contrast prepare the protein chip; and the protein chip comprises a substrate, protein detection indicators and a contrast detection indicator coating, wherein the protein detection indicators are distributed as arrays on the substrate. Detection liquid of a supplementary experiment reagent is filled in the kit. By adopting the protein chip, the protein profiles of three types of people, namely, normal people, people subjected to esophageal squamous cell carcinoma precancerous lesions, and people subjected to the esophageal squamous carcinoma, can be determined; and means are provided for screening the esophageal squamous carcinoma in the early stage, diagnosing in mid stage and late stage, as well as monitoring the state of illness.

Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and / or late stage HPV-associated or HPV-specific cancers. The anti-HPV antibodies are used in performing immunological assays on clinical samples. Various immunological assays and kits for detecting HPV infection, cervical cancer, other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression are also provided.

Uses of topical compositions comprising a 2-hydroxycarboxylic acid or related compound to alleviate or improve signs of skin, nail and hair changes associated with intrinsic or extrinsic aging are disclosed. 2-Hydroxycarboxylic acids and their related compounds include, for example, 2-hydroxyethanoic acid, hydroxypropanoic acid, 2-methyl 2-hydroxypropanoic acid, 2-phenyl 2-hydroxyethanoic acid, 2-phenyl 2-methyl 2-hydroxyethanoic acid, 2-phenyl 3-hydroxypropanoic acid, 2,2-diphenyl 2-hydroxyethanoic acid, 2-hydroxybutane-1,4-dioic acid, 2,3-hihydroxybutane-1,4-dioic acid, 2-carboxy 2-hydroxypentane-1,5-dioic acid, 2-ketopropanoic acid, methyl 2-ketopropanoate, ethyl 2-ketopropanoate, and gluconolactone. Topical application of compositions comprising 2-hydroxycarboxylic acid and / or related compounds has been found to alleviate or improve skin lines; blotches; blemishes; nodules; wrinkles; pigmented spots; atrophy; precancerous lesions; elastotic changes characterized by leathery, coarse, rough, dry and yellowish skin; and other skin changes associated with intrinsic aging or skin damages caused by extrinsic factors such as sunlight, radiations, air pollution, wind, cold, dampness, heat, chemicals, smoke and cigarette smoking. Topical applications of such compositions have also been found to improve the overall qualities of nail and hair affected by intrinsic aging or damaged by extrinsic factors.

The invention discloses an esophageal cancer pathological image processing method based on depth learning, which comprises the following steps: a) pathological slice scanning; B) Encircling the epithelial region type, encircling the normal region of the epithelial region, the low-grade and high-grade precancerous lesion region; (c) image preprocess to obtain small epithelial images; (d) divide each epithelial small image into n image blocks along its longitudinal direction by a convolution neural network, and carry out feature extraction on each image block; E) a long-term and short-term memory network LSTM for acquiring feature vectors of small epithelial images; F) Classifier classification; G) Modeling and tuning, h) Accuracy calculation. The esophageal cancer pathological image processing method of the invention After processing by CNN, LSTM network and classifier, the probability of each epithelial small image being normal, low-grade and high-grade precancerous lesions is obtained, which provides an effective digital image processing method for the scientific utilization of pathological esophageal cancer slices. The method has remarkable beneficial effect and is suitable for application and popularization.

The present invention relates to methods for treating and preventing cancer and for treating precancerous lesions by administering a therapeutically effective dose of a vaccine comprising fusion cells formed by fusion of antigen presenting cells and non-dendritic cells that contain genomic DNA or cDNA derived from a tumor cell or a pre-cancerous cell to a cancer patient or patient with a precancerous lesion. In certain embodiments, such vaccines are administered in combination with a cytokine or other molecule that stimulates a cytotoxic T cell (CTL) response and / or a humoral immune response. The present invention also relates to methods for treating and preventing an infectious disease by administering a therapeutically effective dose of a vaccine comprising fusion cells formed by fusion of antigen presenting cells and non-dendritic cells that contain genomic DNA or cDNA derived from the infectious agent that causes the infectious disease to be treated or prevented to a subject. The present invention also related to methods for producing the fusion cells to be used with the methods of the invention. The present invention also provides compositions comprising the fusion cells to be used with the methods of the invention.

The invention belongs to the field of biomedicine and relates to a primer probe set for early diagnosis, detection or screening of colorectal cancer and precancerous lesions thereof, comprising at least two groups selected from the following groups of primers and probes: a) a forward primer, a reverse primer, a blocker primer and a probe for a methylated SDC2 gene, b) a forward primer, a reverse primer, a blocker primer and a probe for a methylated SFRP2 gene, c) a forward primer, a reverse primer, a blocker primer and a probe for a methylated SFRP1 gene, d) a forward primer, a reverse primer,a blocker primer and a probe for a methylated SEPT9 gene, and e) a forward primer, a reverse primer, a blocker primer and a probe for a methylated PRIMA1 gene. In the invention, the primer probe setand kit simultaneously detect a plurality of methylation markers specific to colorectal cancer and colorectal advanced adenoma, which can improve the detection sensitivity of colorectal cancer and precancerous lesions thereof and minimize the complexity of operation to the greatest extent.

Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and DNA chips were produced by using the oligonucleotide probes. The synthesis of the oligonucleotide probes is based on clones of Ll and E6 / E7 genes of 35 types of HPV obtained from cervical cell specimens from 4,898 Korean adult women and tissue specimens from 68 cervical cancer cases in addition to information based on American and European cases. The DNA chips can analyze the 40 types of HPV found in cervical, diagnose complex infection by at least one type of HPV, and have excellent diagnostic sensitivity and specificity on HPV genetic type up to 100% and reproducibility. Also, the DAN chips are superior to conventional analytic method, and very economical, since they can analyze numerous specimens in shortest time. Accordingly, the DNA chips are useful for predicting cervical cancer and precancerous lesion.

The invention relates to a human papilloma virus capsid protein L1 (HPVL1) peptide and preparation and application thereof. The HPVL1 peptide is a peptide segment of which the amino acid of the sequence is N end-Cys Thr Leu Thr Ala Asp Val Met listed Thr Tyr Ile His-C end, or is a peptide segment comprising the amino acid of the sequence and with a length less than 50 amino acids. In the invention, the peptide which is a very conservative peptide segment located on the surface of protein is sieved out by performing epitope forecasting and multi-sequencing comparison on the L1 proteins of 18 low-risk types and high-risk type HPV subtypes (6, 11, 16, 18, 52, 58 and the like); the antibody generated from induction can react with multiple types of HPVL1, and can be used for the detection of the multiple types of HPVL1, in particular to the application of precancerous lesions detection of human cervical carcinoma; and the antibody aiming at the peptide segment also can be used for purifying and preparing the multiple types of HPVL1. In addition, the invention also provides the applications of the peptide segment used as multiple types of HPV antiserum and detected matters of monoclonal antibody.

The present invention relates to a pharmaceutical composition for treating a neoplasm, a preneoplasm, a proliferative disorder and / or a precancerous lesion, the use of such compositions for the treatment of the listed conditions, and methods of treating the listed conditions. The composition of the present invention comprises an inhibitor of eIF4E, a methltransferase inhibitor, and a pharmaceutically acceptable carrier.

In part, the present invention relates to a method of treating lung diseases and pre-lung disease conditions such as precancerous lesions comprising administering to a patient in need a therapeutic agent comprising lipid composition. The present invention also relates to an inhalation device for administering lipid complexes comprising therapeutic agents. The inhalation device may be disposable. In one embodiment, the lung diseases pretreated by the methods of the present invention are those diseases associated with tobacco related products. The present invention also relates to a method of preparing liposomes by an infusion method that yields high entrapment percentages.

The invention relates to an application of microRNA in preparation of a kit for diagnosis of cervical carcinoma or precancerous lesions. According to the invention, obvious low expression of miR-375 and miR-424 in tissues and cells of cervical carcinoma or precancerous lesions is revealed, and the low expression is obviously relative to the cervical carcinoma or precancerous lesions. The invention also provides a cervical carcinoma or precancerous lesions diagnosis method and the kit for determining existence amount of miR-375 and / or miR-424.

A optical Biopsy method and an apparatus are used in the diagnosis of precancerous lesion for locating the place and determining the level of malignant tumor. The apparatus comprises light source (1, 10) a light channel system, an endoscope (21) and a circuit system. The light sources include an excited light (1) and a cold light source (10). The cold light source and the excited light in the light channel system go through the end of the light guide of the endoscope via optical fiber bundle and irradiate the tested living tissue (22). The white light image signal and the intrinsic fluorescence image signal reflected from the tested living tissue (22) are received by a weak fluorescence CCD (6) that tightly connects to the end of the endoscope (21) and then transmit to the circuit system via a signal wire (9) to produce the image in the display (17). The weak fluorescence signal reflected from the tested living tissue (22) is transmitted to the circuit system via the weak fluorescence fiber bundle (4) protruding from the forceps hole of the endoscope to produce the spectrum image (16).

The invention relates to a non-invasive detection system of precancerous lesions of the digestive tract. The system comprises a human-computer interface, a controller, a wireless energy supply sub system and a robot located in human body, wherein the robot comprises a white light / fluorescent image collecting module which can collect digestive tract image information of the patient detected, the human-computer interface is respectively connected with the controller and the wireless energy supply sub system through connects, the wireless energy supply sub system provides energy to the robot by generating an alternating magnetic field through an energy transmitting ring, the energy is received by the energy receiving ring of the robot, and the controller outputs a control command to the robot through wireless communication and receives data of the robot. The operating mechanism of the robot autonomously crawl in the intestinal tract, a radial operating mechanism of the robot can expand the intestinal tract to reduce the omission factor of the inspection process. The closed radial operating mechanism does not clamp the intestinal structure, so that the safety of the robot is improved.

Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and / or late stage HPV-associated or HPV-specific cancers. Various monoclonal antibodies recognizing specific epitope for specific HPV protein or HPV type, common epitope for various HPV proteins or HPV types are obtained. These obtained monoclonal antibodies are useful tools in early clinical detection of HPV infection and general detection of HPV related diseases, specific detection of invasive cervical cancer, detection of other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression.

The invention discloses a method for inducing a rat liver cancer model by injecting diethyl nitrosamine DEN in coordination with microcystin MC-LR into a rat body through intraperitoneal injection. Indexes such as liver weight to body weight ratio, liver appearance, liver histopathological slicing and GST-Pi (Glutathione S-transferase) protein expression level clearly show liver cell injury phase, liver cell hyperplasia-hepatocirrhosis phase and liver cell carcinomatous change process in a rat liver cancer occurring process, and find that the liver cancer model induced by the DEN in coordination with the microcystin MC-LR has a more obvious effect at the same time than that induced by DEN independently; the tumor formation time is shorter; and the model is an ideal model for researching occurrence of the liver cancer of a human body. The model has the characteristic of clearly showing each stage for occurrence of the liver cancer and contributing to research of a liver cancer occurrence process mechanism, detection of precancerous lesion and development of treatment medicaments.

The invention discloses a Chinese medicinal formula for treating atrophic gastritis and precancerous lesion. The effective ingredients comprise the following Chinese medicinal herbs by weight: 10 to 50 grams of sauted pilose asiabell root, 10 to 50 grams of sauted large-headed atractylodes rhizome, 10 to 30 grams of tuckahoe, 5 to 20 grams of pinellia tuber, 5 to 20 grams of dried orange peel, 3 to 10 grams of raw licorice root, 5 to 15 grams of root powder of pseudo-ginseng, 5 to 20 grams of zedoary, 5 to 20 grams of Zhejiang fritillaria bulb, 15 to 60 grams of spreading hedyotis herb, 10 to 60 grams of hairy-vein agrimonia herb and bud, 10 to 60 grams of barbed skullcap herb, 5 to 20 grams of fruit of princesplume ladysthumb and 5 to 20 grams of creeping fig fruit. The Chinese medicinal formula has the effects of tonifying spleen, regulating qi, dissipating blood stasis and detoxifying, treats both principal and secondary aspects of diseases aiming at the pathogenesis of the chronic atrophic gastritis and precancerous lesion, has low recurrence rate after healing and good short-term and long-term curative effect, and has not toxic or side effect.

The invention belongs to the field of prognosis estimation and canceration risk prediction on clinic of molecular biology, and particularly relates to application of a molecule of a detection ANO1 protein in the preparation of a kit or a detection agent for the prognosis estimation and precancerous disease progression risk prediction of esophageal squamous carcinoma. The molecule expressed by the detection ANO1 protein can be a nucleic acid, a protein or a compound, thus showing that the ANO1 protein can be used as a molecular marker for the prognosis estimation and precancerous disease progression risk prediction of the esophageal squamous carcinoma.

A method is disclosed for treating hyperproliferative body surface lesions, including cancerous or precancerous lesion, such as warts or anogenital cancers, by applying a polyaryl mononitro- or dinitrophenylhydrazone such as (I) wherein R1 is hydrogen, hydroxy, 2- or 4-hydroxyphenyl, acetate, nitroso, phosphate, azido, nitrile, amino, dimethylamino, sulfate, methylsulfonate, phosphate, succinate or another water soluble electrophilic group capable of hydrogen bonding; R2 is C6H4OH, C6H4N3, C6H4CN, 4-HO—C6H4—C6H4, C6H4OPO2OH, C6H4OSO2H, C6H4NH2, C6H4NHMe2, C6H4OSO2Me, C6H4OCO(CH2)xCO2H, or C6H5Cl; and X is C6H3-2,4(NO2)2, C6H4-4(NO2), C6H4-3(NO2), or C6H3-2,4(NO2)2. In a particular example, R1 is OH, R2 is C6H4OH and X is C6H3-2,4(NO2)2.

The invention relates to an improved cervical squamous epithelial cell staining method, a cervical squamous epithelial cell staining kit and application of the method and the kit to diagnosis of individual cervical squamous epithelial cell cancers. The improved cervical squamous epithelial cell staining method comprises the steps of staining cervical squamous epithelial cells by using cell acid phosphatase staining reagent and staining the cervical squamous epithelial cells by using Pap staining reagent. The cervical squamous epithelial cell staining kit comprises the cell acid phosphatase staining reagent and the improved Pap staining reagent. The invention additionally relates to a kit for diagnosing individual cervical squamous epithelial cell cancers or precancerous lesions.

The invention discloses a target cell staining kit for displaying the proliferative activity of a cell and a use method thereof. The kit comprises I type and II type which respectively comprise an enzyme substrate, test paper, enzyme stationary liquid, phosphate buffer solution, Mayer hematoxylin and the like. The staining method comprises the steps of preparing pieces, fixing, staining and the like. The method can display acid phosphatase in exfoliative cells on smears such as exfoliative cervical cells, esophagus brush sheet cells and the like and search abnormal proliferated cells according to the target. Compared with the traditional Pasteur smear method, the method has the advantages of higher sensitivity for detecting cancer and precancerous lesions and similar specificity, therefore, the invention can be used for cytology screening of cervical carcinoma, esophageal cancer, lung cancer and other malignant tumors.

The invention provides a noninvasive examination device for gastrointestinal precancerous lesions. The noninvasive examination device comprises a gastrointestinal intracavity white light / fluorescence image acquisition and wireless transmission micro-robot examination subsystem, a lying-in-bed driving subsystem, a human-computer interface control subsystem and a wireless energy supply subsystem. Through a wireless energy supply and active control system, the noninvasive examination device can be controllable in a gastrointestinal tract of a testee and can be used for examination for a long time, examination results can be sent outside the body of the testee in a wireless mode, and consequently noninvasive and painless examination of the whole digestive tract can be achieved.

Embodiments of the invention provide methods, polyclonal antibodies, monoclonal antibodies, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and / or late HPV-associated or HPV-specific proteins or antibodies. Mononoclonal antibodies are used to detect oncogenic high risk and low risk HPV types in a single assay, which is not limited to assay type or format. Useful tools for specific detection of various HPV associated cancers are provided. HPV associated cancer biomarkers are identified and can be used in a screening method for early stage precancerous lesions as well as late stage cancer progression.

The invention discloses a multi-spectrum nasal cavity endoscope real-time image enhancement method and an endoscope imaging system. Bluetooth channel information is used for enhancing the effective information of an image on the basis of airspace filtering, ineffective information is weakened so as to intensify a tumor lesion phenomenon, the endoscope diagnosis accuracy of precancerous lesions and early tumors can be subjected to leap improvement, and missed diagnosis is avoided. Meanwhile, on a premise that a processing effect is not affected, the image is subjected to multi-scale airspace filtering so as to achieve an effect on low-time-delay output and real-time operation.