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Improved histiocyte staining method and application thereof

A staining method and cell technology, applied in the preparation of test samples, etc., can solve the problems of high false positive rate and false negative rate of Papanicolaou staining

Active Publication Date: 2013-04-10
ANHUI NEW MARK SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to many factors, the false positive rate and false negative rate of Pap staining are too high

Method used

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  • Improved histiocyte staining method and application thereof
  • Improved histiocyte staining method and application thereof
  • Improved histiocyte staining method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1 Fixed

[0103] regular fixed :

[0104] Fix the smear (when wet) in 95% alcohol or an equal mixture of 95% alcohol and ether for at least 15 minutes.

[0105] Place them in 80%, 70%, and 50% alcohol for half a minute. Then put in and distilled water for more than 2 minutes.

[0106] special fixative

[0107] Specimen smear fixation: take 7.5ml A solution and 42.5ml B solution and mix to form a special fixative solution. Quickly put the specimen smear into the fixative solution for 50 seconds, take it out and immediately rinse it up and down 10 times in a purified water tank. Can be stored in the refrigerator at 4°C for 2 weeks.

Embodiment 2

[0108] Example 2 Dyeing

[0109] 1. Traditional Pap staining steps

[0110] (1) Stain the fixed sample smear with hematoxylin for 5-10 minutes until the nucleus coloring is obvious, and then rinse it with distilled water.

[0111] (2) Use 0.25% or 0.5% hydrochloric acid alcohol to remove excess hematoxylin in the cytoplasm, until the smear turns light red, rinse it with distilled water immediately, and the operation must be rapid, usually within a few seconds. If the decolorization time is too long, all the hematoxylin will disappear.

[0112] (3) Alkaline in a saturated lithium carbonate solution for about 1 minute until the smear turns light blue, then wash with distilled water.

[0113] (4) Place them in 50%, 70%, and 80% alcohol for half a minute. Then place in 95% alcohol for at least 2 minutes to dehydrate.

[0114] (5) Dye in orange G-6 staining solution for 3 minutes.

[0115] (6) Rinse up and down 6 times in two 95% alcohol tanks respectively.

[0116] (7) Dye i...

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Abstract

The invention relates to an improved cervical squamous epithelial cell staining method, a cervical squamous epithelial cell staining kit and application of the method and the kit to diagnosis of individual cervical squamous epithelial cell cancers. The improved cervical squamous epithelial cell staining method comprises the steps of staining cervical squamous epithelial cells by using cell acid phosphatase staining reagent and staining the cervical squamous epithelial cells by using Pap staining reagent. The cervical squamous epithelial cell staining kit comprises the cell acid phosphatase staining reagent and the improved Pap staining reagent. The invention additionally relates to a kit for diagnosing individual cervical squamous epithelial cell cancers or precancerous lesions.

Description

technical field [0001] The application relates to histological and cytological staining methods and applications thereof, in particular to cervical squamous epithelial cell staining methods and applications thereof. Background technique [0002] Cervical cancer is one of the common gynecological malignancies, and its incidence ranks the second among female malignancies in the world. There are 500,000 new cases of cervical cancer every year, 80% of which occur in developing countries. There are 131,500 new cases of cervical cancer every year in China, accounting for 28.8% of the total number of new cases of cervical cancer in the world. In recent years, studies have found that the number of young cases of cervical cancer is increasing year by year, the age of onset is younger, and the occurrence and development of cervical cancer has a process of quantitative change, gradual change and mutation. Therefore, early detection, early diagnosis and Early treatment of cervical canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 李戈赵艳梅
Owner ANHUI NEW MARK SCI TECH
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