9 results about "Rectal epithelium" patented technology

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The three-dimensional propaganda and education model comprises a model main body, an identification assembly and a supporting base, the model main body is made of a transparent material, and an unqualified sputum specimen simulation unit (such as saliva and a polluted sputum specimen) and a qualified sputum specimen simulation unit (such as white viscous sputum, yellow viscous sputum and bloody sputum) are arranged in the model main body; each unit is of an independent cavity structure, and the sputum character is visually displayed by accurately controlling the color and viscosity and simulating particles (such as squamous epithelial cells or inflammatory cells); the identification assembly is used for clearly marking the type and the qualified standard through an identification board and a fluorescent coating; the supporting base provides stable placement. Through materialized comparison, the patient can quickly distinguish the sputum type, so that the retention quality is improved from the source, the percent of pass is remarkably improved, the nursing load is reduced, and the method has the advantages of intuition, standardization, high efficiency, safety and the like.

This utility model discloses a protective anal tube for rectal anastomosis after surgery, relating to the field of medical device technology. It solves the initial problem of the need to develop a protective anal tube for rectal anastomosis after surgery that has three-dimensional isolation, facilitates negative pressure drainage, and has a biological barrier function. The utility model includes a tube body with two support members on it for supporting the intestine. Both support members are capable of mechanical deformation, and the contact surfaces between the two support members and the intestine are annular, which can support both sides of the intestinal anastomosis. Thus, a cavity is formed between the two support members and the anastomotic segment of the intestine. The purpose is that the two support members form a physical barrier through the annular contact surfaces, completely preventing the leakage of intestinal contents into the anastomotic area, avoiding contamination of the wound by feces and digestive juices, maintaining a moist environment, and promoting the migration of epithelial cells and the growth of granulation tissue.

The present invention relates to a medium composition for forming a laryngeal organoid and a method for preparing a laryngeal organoid by using same. The method for preparing a laryngeal organoid in which laryngeal epithelial cells are three-dimensionally cultured using the medium composition for forming a laryngeal organoid according to the present invention enables stable long-term serial passaging for two or more generations, so that a large quantity of cells can be obtained from a small amount of tissue. In addition, the laryngeal organoid prepared by the method includes a stratified squamous epithelium structure or pseudostratified ciliated columnar epithelium structure that is histologically highly similar to the larynx, and thus may be used as a graft material for laryngeal tissue or as a model capable of replacing cell and animal experiments in disease modeling and drug screening.

Provided is a method for producing renal collecting duct principal cells, including culturing ureteric bud cells or cells that have been differentiated from ureteric bud cells in a medium comprising a TGFβ inhibitor, a Wnt inhibitor, and a phosphodiesterase inhibitor. Also, provided is a method for producing a renal collecting duct principal cell-containing organoid, including culturing a ureteric bud organoid or an organoid that has been differentiated from a ureteric bud organoid in a medium comprising a TGFβ inhibitor, a Wnt inhibitor, and a phosphodiesterase inhibitor.

The application discloses a human head and neck squamous cell carcinoma clinical diagnosis reagent, which is a reagent for detecting the expression amount of a human NCAPH gene. The expression level of the human NCAPH gene is negatively correlated with the clinical prognosis of human head and neck squamous cell carcinoma. Experimental results show that the expression of the human NCAPH gene in a human head and neck squamous cell carcinoma cell line is higher than that in normal nasopharyngeal epithelial cells. Knocking down the expression of the human NCAPH gene can significantly inhibit the proliferation and colony sphere formation ability of the head and neck squamous cell carcinoma cells. The application also provides a drug for inhibiting the expression of the human NCAPH gene or a NPIDP small peptide drug for inhibiting the expression of the human PD-L1 gene. The NPIDP small peptide drug is used on human head and neck squamous cell carcinoma cells, can significantly compete to block the combination of NCAPH and HIP1R, promote the degradation of the PD-L1 protein, and thus enhance the T cell killing effect. The application provides a new diagnosis and treatment drug for the treatment of human head and neck squamous cell carcinoma.

The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation / maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

The invention provides application of C. sporogenes or supernate thereof in preparation of a medicine for preventing and / or treating Graves disease, and belongs to the technical field of biological medicine. The research finds that after intervention of C.sporogenes and supernate thereof, thyroid hormones FT4 and TRAb of a mouse suffering from Graves disease are obviously reduced, TSH is increased, serum inflammation anti-inflammatory factors are increased, proinflammatory factors are reduced, body weight is increased, and food intake is reduced; the colon tissue structure is complete, columnar epithelial cells of the epithelial layer are arranged in order, cup-shaped cells are evenly distributed and moderate in number, colon villus is complete, a small amount of panesian cells can be seen in crypts, thyroid tissue follicles are normal in structure and are oval or round, the follicles are different in size, the epithelial cells are in a single-layer flat shape and are arranged in order, and the follicles contain rich colloid. The Graves disease can be effectively treated.