8 results about "Vaginal mucosa" patented technology

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The patent discloses a kind of vaginal injection devices, it is related to medical injection field, device includes base and expansion board, expansion board can slide along the sliding slot on base, reach the function of vaginal speculum by the expansion of expansion board, make expansion board adhere to vaginal mucosa;Base has handle for fixing entire injection device;Expansion board has several needle holes, injection needle can be worn from needle hole and carry out drug injection;Needle and medicine bin are connected, medicine bin and needle are closed space, fill the medicine needed to be injected in;The length of needle that entire medicine bin is adjusted to be worn needle hole is adjusted by the movement of gear and gear sliding slot, the depth of drug injection into vaginal mucosa;The new type of patent does not need to use vaginal speculum to assist injection in vagina during use process, can accurately control the depth of drug injection, complete the injection of entire vaginal mucosa once, shorten operation time, improve injection efficiency, reduce drug waste, improve curative effect, reduce patient pain.

This invention discloses a compound preparation of plant-fermented lactic acid bacteria lysate for nourishing and protecting the vaginal pelvic floor muscles. By weight, it comprises the following components: kidney bean-honeysuckle fruit compound ferment: 4-8 parts; pomegranate peel-derived lactic acid bacteria lysate: 5-9 parts; bamboo fungus mycelium extract: 3-7 parts; compound sophorolipid: 6-8 parts; sodium hyaluronate: 2-5 parts; total glycosides of Centella asiatica: 1-3 parts; glycerin: 5-10 parts; lactic acid: 1-2 parts; purified... Water: 36-45 parts; Carbomer 940: 2-4 parts; Trehalose: 1-3 parts; Chitosan: 1-3 parts; Methylchloroisothiazolinone: 0.1-0.3 parts; Sodium hydroxide: 0.1-0.2 parts; This invention can regulate the vaginal microecological balance, nourish the vaginal mucosa, enhance the toughness of pelvic floor muscle fibers, and promote the repair of pelvic floor muscle damage, solving the technical problems of existing preparations having single function, weak targeted pelvic floor muscle care, and easy disruption of the vaginal microecology.

This invention discloses a gynecological antibacterial hydrogel, its preparation method, and its application. The hydrogel comprises a water extract of traditional Chinese medicines including Verbena officinalis, Salvia miltiorrhiza, Portulaca oleracea, Sophora flavescens, Viola yedoensis, Phellodendron chinense, and Artemisia argyi, as well as lactic acid, chitosan, and water. The preparation involves a water extraction and alcohol precipitation purification process to obtain the herbal extract, which is then mixed with a chitosan-lactic acid swelling matrix, adjusted to pH 3.8-4.4, and then filled and sterilized. Based on the principles of traditional Chinese medicine (TCM) formulation and combined with modern pharmaceutical technology, this invention's gel possesses the effects of clearing heat and drying dampness, detoxifying and killing parasites, and promoting blood circulation and removing blood stasis. It exhibits strong inhibitory activity against common gynecological pathogens, restores the acidic environment of the vagina, regulates local immunity and microenvironment, has an adjunctive therapeutic effect on HPV infection, is non-irritating to the vaginal mucosa, and has high safety and good stability.

This application discloses an in vitro reconstructed vaginal mucosa model, its construction method, and its application. The construction method includes an improved culture medium: HVU medium is added to immortalized vaginal epithelial cell line VK2 / E6E7, which is then seeded into Transwell chambers and cultured. The HVU medium on the surface of the culture in the Transwell chambers is aspirated, and the Transwell chambers are raised to the air-liquid interface for air-liquid surface culture. The culture medium is then replaced with HVA medium to obtain the vaginal mucosa model. This method improves the stability of cells during in vitro culture and constructs an in vitro reconstructed vaginal mucosa model with a tissue structure highly similar to human vaginal mucosa (possessing a complete stratified squamous epithelial structure and normal intercellular connections), thereby enhancing the model's physiological functional simulation.

This invention discloses a strain of *Lactobacillus johnsonii* and its metabiotic that simultaneously improves vaginal mucosal damage and inhibits vaginal inflammation, belonging to the field of microbial technology. The *Lactobacillus johnsonii* strain of this invention was isolated from vaginal secretions of healthy women. It has the effects of improving vaginal mucosal damage and inhibiting vaginal inflammation, specifically manifested in: promoting the migration and repair of vaginal epithelial cells; reducing the content of soluble cadherin E in the vaginal mucosa of mice and increasing the expression of vaginal tight junction protein OCLN; reducing the shedding of vaginal mucosal epithelial cells; reducing the secretion of MPO and IL-1β in mice with vaginitis; and (6) improving the pathological characteristics of the vagina in mice. Therefore, this *Lactobacillus johnsonii* has great application potential in products that improve vaginal mucosal damage and inhibit vaginal inflammation.

The invention provides a female external nursing product with antibacterial, anti-inflammatory and antipruritic effects and a preparation method thereof, and relates to the technical field of biological medicine nursing products. The invention provides an external nursing product for women, which is prepared from the following pure traditional Chinese medicinal materials: cortex ailanthi, saururus chinensis, pericarpium granati, chingma abutilon seeds, red halloysite, lysimachia clethroides, gynura plicata, evodia lepta leaves, peach leaves, pine needles, water gossypium, solanum lyratum and cyrtomium fortunei as raw materials. The traditional Chinese medicine raw materials in the product are scientific in compatibility and have a synergistic effect, the product shows an ultra-excellent antibacterial effect on staphylococcus aureus, escherichia coli and candida albicans, meanwhile, the product has good anti-inflammatory and anti-inflammatory effects, can achieve rapid itching relieving, and in addition, the product has no stimulation effect on vaginal mucosa of female white rabbits and has good safety. The product disclosed by the invention can be used for effectively improving the symptoms such as itching and causalgia, peculiar smell and abnormal secretion caused by colpitis mycotica, bacterial vaginitis, trichomonas vaginitis, senile vaginitis and the like.