66 results about "Leucine" patented technology

The invention provides a sustained-release pellet containing gamma-aminobutyric acid, the sustained-release pellet comprises a pellet core and a coating layer wrapping the pellet core, the pellet core contains a physiologically active substance, and the physiologically active substance is gamma-aminobutyric acid; the pellet core comprises the following components in parts by weight: 15-40 parts of gamma-aminobutyric acid, 30-55 parts of a filling agent, 1-30 parts of a lubricating agent and 0.5-5 parts of an anti-caking agent; the filling agent comprises at least one of microcrystalline cellulose, powdery cellulose, starch and derivatives thereof, hydroxypropyl cellulose, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sugar or sugar alcohol and fruit and vegetable powder; the lubricant comprises at least one of talcum powder, magnesium stearate, stearic acid, glycerol and leucine; the anti-caking agent comprises at least one of magnesium carbonate, tricalcium phosphate, calcium hydrophosphate and silicon dioxide.

The application belongs to the technical field of protein extraction, and provides a method for improving water holding property and oil holding property of peony seed protein, the core of the method is that before the extraction of the peony seed protein, the peony seed is pretreated by the following method: the peony seed meal is subjected to steam explosion treatment under the condition of 0.4-1.2 Mpa and 60-150 s, preferably 0.8 Mpa and 100 s, to obtain the steam exploded peony seed, and the sample after the steam explosion is subjected to drying treatment; preferably heat pump drying. The peony seed protein prepared by the method has obviously improved water holding property, oil holding property, foaming property, foam stability, emulsifying property and emulsion stability; the valine is increased by 8.58%, the leucine is increased by 6.63%, and the tyrosine is increased by 10.65%; the extraction rate of the seed protein is 76.82%, and the extraction effect is excellent.

The invention relates to the technical field of fermentation engineering and wine brewing, and discloses a low-alcohol fruit wine brewing process based on compound microorganism synergistic fermentation, which comprises the following steps: inoculating Meijie yeast into clarified fruit juice, adding L-leucine, culturing under controlled temperature, and removing free iron ions by biological chelation until the liquid level becomes red; then inoculating saccharomyces cerevisiae, starting a trace oxygen introduction and ORP (oxidation-reduction potential) system after main fermentation is started, and controlling potential to carry out metabolism redirected fermentation in a micro-aerobic interval until residual sugar reaches the standard; after the fermentation is finished, adding an alkaline regulator to increase the pH value, inducing the haematochrome-iron compound to flocculate and settle, separating to remove the precipitate, and adding an acidic regulator to adjust the pH value to obtain the low-alcohol fruit wine. According to the method disclosed by the invention, a brewer's yeast respiratory chain is blocked and oxidative stress is applied by utilizing a metabolic redirection strategy under precursor-induced biological iron removal and micro-aerobic environment, so that a carbon flux is promoted to flow to a glycerol synthesis path, the ethanol yield is reduced, and the glycerol content is greatly increased.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

This invention provides a novel raw material that, for cats, which exhibit unique characteristics in their kidneys compared to other mammals, can inhibit the decline in feline kidney function and improve coat condition while maintaining the form of cat food or other feeds. When using a poorly soluble soybean protein raw material with a characteristic amino acid composition of less than a glutamic acid (Glu) to leucine (Leu) mass ratio of 2, and an NSI of 10%–60%, the decline in feline kidney function can be inhibited and coat condition improved through small-scale intake. This raw material can also be obtained as undigested residue from the soybean peptide manufacturing process.

This invention discloses a *Lactococcus faecium* strain, YS-IM01. This strain can efficiently hydrolyze sheep colostrum proteins and specifically release a large amount of leucine. This invention also provides a polypeptide composition comprising multiple active peptides with potential for muscle synthesis, immunomodulation, and antioxidant activity. This invention further provides a fermented sheep colostrum product obtained by fermenting sheep colostrum with the aforementioned *Lactococcus faecium*. This invention also provides a method for preparing the fermented sheep colostrum product, comprising steps including fermentation, low-temperature vacuum concentration, and vacuum freeze-drying. Simultaneously, this invention also provides a method for preparing a drug using *Lactococcus faecium* and the polypeptide composition to prevent and / or improve age-related muscle loss.

The invention relates to the technical field of amino measurement, in particular to a method for measuring the content of reactive primary amino in sulfydryl-containing protein and application of the method. The determination method comprises the following steps: S1, weighing a proper amount of L-leucine solid, dissolving the L-leucine solid in ultrapure water, preparing the L-leucine solid into different concentrations by adopting a NaHCO3 solution, then determining the content of the reactive primary amino group by adopting a TNBS method, and establishing a standard curve according to the relationship between an OD value at the maximum absorption wavelength and the concentration of the reactive primary amino group; s2, carrying out sealing treatment on the to-be-detected protein containing the sulfydryl by adopting N-ethyl maleimide; and S3, determining the content of the primary amino group of the protein by adopting a TNBS method, and substituting the OD value at the maximum absorption wavelength into the standard curve to obtain the concentration of the reactive primary amino group. According to the method, reactive sulfydryl in the protein is blocked in advance through N-ethylmaleimide to eliminate interference of sulfydryl, and the content of reactive primary amino groups in the protein can be measured more accurately.

The invention discloses a Lactobacillus paracasei 1029 and application thereof, the preservation number of the Lactobacillus paracasei 1029 is CCTCC (China Center for Type Culture Collection) NO: M20251441, and the Lactobacillus paracasei 1029 is preserved in the China Center for Type Culture Collection on June 23, 2025. The fermentation product contains a plurality of active metabolites, such as pyruvic acid, pantothenic acid, proline and leucine, and the components synergistically enhance the effects of oxidation resistance, bacteriostasis and cell melanin inhibition. Compared with the prior art, the fermented product disclosed by the invention realizes integration of multiple effects, is high in safety (free of cytotoxicity) and simple in preparation process (standing for 6 days at 30 DEG C, filtering, centrifuging, freezing and drying), has wide application prospects including food additives, health-care products, cosmetics and pharmaceutical preparations, and can remarkably improve the bioavailability and market competitiveness of products.

This invention provides the application of a cyclic dipeptide in inhibiting the TRPV1 protein, wherein the cyclic dipeptide is a cyclic (D-leucine-L-proline) dipeptide. This invention also relates to the application of the cyclic dipeptide in the preparation of topical skin agents or pharmaceuticals with TRPV1 protein inhibitory activity, wherein the topical skin agent is selected from: creams, lotions, gels, toners, serums, masks, eye creams, aerosol cleansing foams, sprays, shower gels, or facial cleansers.

Delayed-release micropellet particles containing γ-aminobutyric acid, characterized in that it comprises a pellet core and a coating layer surrounding the pellet core, wherein the pellet core contains a physiologically active substance, the physiologically active substance being gamma-aminobutyric acid; the pellet core comprises, calculated in parts by weight, the following components: 15 to 40 parts of gamma-aminobutyric acid, 30 to 55 parts of a filler, 1 to 30 parts of a lubricant, 0.5 to 5 parts of an anti-caking agent; wherein the filler comprises at least one of microcrystalline cellulose, powdered cellulose, starch and its derivatives, hydroxypropyl cellulose, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sugar or sugar alcohols and solid fruit and vegetable drinks; wherein the lubricant comprises at least one of talc, magnesium stearate, stearic acid, glycerol and leucine; wherein the anti-caking agent comprises at least one of magnesium carbonate, tricalcium phosphate, dicalcium phosphate and silicon dioxide.

The application discloses a novel insect short neuropeptide sNPF analogue and application thereof in pests, and relates to the technical field of agriculture; the application provides a structural general formula of the novel insect short neuropeptide sNPF analogue, and the novel insect short neuropeptide sNPF analogue is applied to the prevention and treatment of aphids, thrips, Plutella xylostella and Spodoptera frugiperda. The terminal tyrosine Y, arginine R or leucine L residue is substituted and modified by using hydrophilic, hydrophobic, steric hindrance natural amino acids or unnatural amino acids and other groups on a lead compound, and the obtained novel insect short neuropeptide sNPF analogue can be used in the prevention and treatment of various pests.

The invention discloses application of a soybean GmCDF3 gene in regulating and controlling the content of seed protein, amino acid and raffinose, and relates to application of soybean GmCDF3. According to the application of the soybean GmCDF3 gene in regulating and controlling the contents of seed protein, amino acid and raffinose, the application method is to culture plant seeds by using the soybean GmCDF3 gene, and further improve the plant quality. According to the invention, a GmCDF3 overexpression vector is constructed by utilizing a molecular means, and the soybean GmCDF3 gene is overexpressed in soybean through an agrobacterium-mediated genetic transformation technology, so that the content of 12 amino acids such as cysteine, leucine, lysine, phenylalanine and threonine in soybean seeds can be remarkably increased, and the yield of soybean seeds is increased. The protein content of the seeds is obviously improved, the oil content and the grain weight are not influenced, and the raffinose content is obviously reduced. Meanwhile, the soybean GmCDF3 gene is over-expressed in arabidopsis thaliana, and the protein content of arabidopsis thaliana seeds is also remarkably increased.

The present application relates to a kind of compositions for regulating autophagy of eukaryotic cell, improving mitochondrial dysfunction and its preparation method and application.It includes the following weight parts of raw materials: 10-30 parts of broccoli powder, 10-30 parts of wheat germ microcapsule powder, 20-40 parts of raspberry extract, 20-40 parts of banana powder, 2-6 parts of leucine, 0.1-1 parts of pyrroloquinoline quinone disodium salt.The present application also provides the preparation method of broccoli powder, wheat germ microcapsule powder, raspberry extract and banana powder, and the use of the composition.The composition combines the efficacy of the above 6 ingredients, reasonable compatibility, safe and effective, various components activate mitochondria through regulating protein, PINK1 / Parkin-dependent pathway and PINK1 / Parkin-independent pathway;At the same time, by activating PGC-1 alpha signal pathway, promote mitochondrial DNA replication and new mitochondria generation;Stabilize mitochondrial membrane potential, improve ATP synthesis efficiency;Synergistically activate mitochondrial autophagy, improve mitochondrial dysfunction and then relieve aging.

The invention discloses a method for improving the stable activity and antioxidant activity of chicken peptide ADH, and a polypeptide and application thereof. The ADH stable activity and the antioxidant activity of the chicken peptide can be improved by performing the plastein reaction on the chicken peptide, and it is proved for the first time that the ADH stable activity and the antioxidant activity of the chicken peptide can be further remarkably improved by adding exogenous amino acid in the plastein reaction; compared with the unreacted chicken peptide and the chicken peptide subjected to plastein reaction, the ADH stable activity and the oxidation resistance of the prepared product are remarkably improved by at least 105%; wherein under the plastein reaction action of alkaline protease, cysteine, leucine, tryptophan and lysine, the ADH stable activity and antioxidant activity of the chicken peptide are remarkably improved. The method provided by the invention is simple, convenient and rapid, the prepared polypeptide product has a remarkable effect, and more product raw material sources with better effects are provided.

We provide foods or pet food ingredients, foods, pet food, and supplements that have anti-glycation effects. [Solution] The present invention provides an anti-glycation agent comprising a dipeptide or salt thereof made of Lys-Leu as an active ingredient, a food or pet food material containing the same, a food, a pet food, and a supplement.

The present application belongs to the technical field of organic waste resource utilization, and particularly relates to a composite nutrient agent for regulating and controlling product spectrum of organic waste anaerobic fermentation, a method and application, the composite nutrient agent comprising energy substrate, organic nitrogen source, B group vitamins and mineral buffer elements, wherein: the energy substrate comprises lactic acid, acetic acid, glucose, xylose and arabinose; the organic nitrogen source comprises crude protein containing full-spectrum amino acids, the full-spectrum amino acids comprising aspartic acid, glutamic acid, alanine and leucine; the B group vitamins comprising vitamin B1, vitamin B2 and vitamin B6; and the mineral buffer elements comprising potassium, calcium and magnesium elements. The present application introduces the composite nutrient agent into a chain extension system with organic waste as a substrate, and provides a new regulation strategy and special additive for high-value utilization of organic waste.

The application discloses selenium-rich rice with multiple amino acids and a preparation method and application thereof, and belongs to the technical field of cross between agricultural planting and deep processing of agricultural products. The rice meets four core indexes: the proportion of organic selenium is greater than or equal to 80% (of which the proportion of selenomethionine is greater than or equal to 70%), the index of human essential amino acids is greater than or equal to 0.95, the content of leucine and arginine is significantly strengthened, and the content of characteristic aroma substance 2-AP is greater than or equal to 200 mu g / kg. The preparation method comprises the following steps: at the booting stage of rice, a first nutrient solution with a specific ratio of four kinds of amino acids is sprayed to optimize the metabolic basis; at the early stage of grain filling, a second nutrient solution containing selenomethionine and proline is sprayed to realize synergistic transport; and finally, the harvested rice is subjected to specific temperature and humidity after-ripening treatment. Through unique time sequence design and material combination, the method improves the selenium nutrition, amino acid quality and "roasted nut aroma" flavor of the product, and provides technical support for the development of high-quality rice.

The preparation method comprises the following steps: adding copper sulfate into a PBS (Phosphate Buffer Solution) containing BSA (Bovine Serum Albumin), standing for 2 hours, carrying out centrifugal separation, and drying to prepare BSA-Cu3 (PO4) 2 hybrid nanoflowers, as a nano-enzyme for simulating a binuclear copper active center structure of tyrosinase, the nano-enzyme has the advantages of high surface area, efficient catalytic activity and high stability, then a solid salt hydrate is added to provide active water similar to the action of a cofactor in enzyme catalysis, natural polyphenol is catalyzed to be oxidized in an organic phase to generate an o-benzoquinone compound to serve as a hydrophobic part, and the activity of the o-benzoquinone compound is improved. A primary product obtained by ethanol separation is subjected to a Michael addition reaction with leucine, melanogenesis is simulated, the amino acid surfactant is biomimetic synthesized, and the obtained product has excellent surface performance.

The utility model discloses a filter device, which belongs to the technical field of amino acid production equipment, and comprises a filter box body, a filter cartridge is arranged in the filter box body, one side of the filter box body is provided with a sliding chute I, one side of the filter box body is fixedly connected with a mounting plate, one side of the mounting plate is fixedly connected with an air cylinder, and the air cylinder is fixedly connected with a sliding chute II. By arranging the filter cartridge driven by the air cylinder, the gear and the rack, solid impurities can be poured after being accumulated in the filter cartridge, automatic pouring of the solid impurities in the filter cartridge is achieved, the situation that the filtering effect is affected due to accumulation of the solid impurities is avoided, the automation degree is high, and the labor cost is saved; according to the L-leucine filtering device, the stirring blades driven by the motor and the stirring shaft are arranged, L-leucine can be continuously stirred in the filtering process, the filtering efficiency and effect are improved, solid impurities are prevented from sinking to the bottom and further blocking the filtering cylinder, and when the solid impurities are poured out, the stirring blades can also rotate to improve the discharging efficiency of the solid impurities.

The present application provides a kind of modulator that can improve the expression and yield of Bt cotton insecticidal protein under adversity, ingredients include: glutamic acid, aspartic acid, valine, leucine, alkyl polyglucoside and 6-phosphofructose or water-soluble chitooligosaccharide;The modulator is prepared with deionized water before application, and the preparation method is as follows: alkyl polyglucoside is dissolved in water, 6-phosphofructose / water-soluble chitooligosaccharide is added to dissolve, then aspartic acid, valine and leucine are added to obtain solution A;Glutamic acid is dissolved in water, and alkali is added dropwise to neutral, to obtain solution B;Solution A and solution B are mixed uniformly and diluted with water to obtain the prepared modulator.The growth regulator provided by the present application can increase the content of Bt cotton insecticidal protein after adversity stress, increase the insect resistance of Bt cotton itself, and improve the growth and development of cotton under adversity, realize high yield of cotton under the premise of reducing pesticide use, and provide practical guidance and technical support for improving the economic and ecological benefits of Bt cotton planting.

The invention relates to the technical field of medical treatment, in particular to herbal gynecological inflammation clear water gel, which is prepared from the following components in percentage by mass: 14 percent of traditional Chinese medicine extracting solution, 47.5 percent of matrix and 38.5 percent of matrix, the matrix 1 is N, O-carboxymethylated chitosan, and the matrix 2 is L-leucine. The hydrogel preparation adopted by the invention can be tightly attached to the surface of a vaginal mucosa by virtue of a specific three-dimensional network structure and good elasticity, so that more uniform coating and more lasting retention are realized, the loss of medicinal components caused by the self-cleaning effect of the vagina is reduced, and the effect that the medicine can continuously act on an inflammation part is ensured; the hydrogel prepared by the method is soft in texture, high in biocompatibility, almost free of irritation and foreign body sensation after being used and smoother and milder in administration process, and the comfortable use experience is beneficial to relieving psychological burdens of patients and enhancing trust and cooperation of the patients with treatment schemes.

The invention relates to the field of microorganisms, and discloses recombinant escherichia coli, a construction method and application thereof and a method for producing L-leucine. The recombinant escherichia coli simultaneously contains a coding gene of an overexpressed pyruvic acid transport protein, a coding gene of an overexpressed glucose transport protein and a coding gene of an overexpressed 6-phosphofructokinase, and does not contain a coding gene of fructose-1, 6-diphosphatase. The recombinant Escherichia coli provided by the invention can significantly improve the utilization rate of the raw material glucose, improve the yield of L-leucine and inhibit the generation of byproducts. In a preferred embodiment of the invention, when the recombinant escherichia coli is fermented for 20 hours in a 5L fermentation tank, the yield of L-leucine reaches 70 g / L, the saccharic acid conversion rate reaches 36%, the daily yield is remarkably increased, and the production cost is greatly saved.

The application discloses an Escherichia coli genetically engineered strain for producing pyruvic acid derivative compounds, and discloses a method for improving the yield of pyruvic acid derivative compounds in the Escherichia coli, and discloses the application of the Escherichia coli genetically engineered strain in biosynthesis of poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene. The application can widely enhance the synthetic metabolic flow with pyruvic acid as a key node by knocking out a leucine response regulator gene, and the modification can effectively strengthen the synthesis capacity of the engineered strain for the four target products, i.e., poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene under the condition of various carbon sources. Moreover, the knockout of the gene can specifically promote the lactic acid precursor flux, thereby improving the molar proportion of lactic acid monomers in the product P(3HB-LA). lrp co ​​

The present disclosure relates to methods of treating cancer, such as lung cancer (e.g., non-small cell lung cancer) by administering a dry powder to the respiratory tract of a subject in need thereof (e.g., by oral inhalation), the dry powder comprising respirable dry particles that comprise an angiogenesis inhibitor (e.g., itraconazole), and optionally a stabilizer (e.g., polysorbate 80) and / or one or more excipients (e.g., leucine and a sodium salt, such as sodium sulfate).

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the content of soybean leucine and application of the SNP marker. The SNP marker is located at the S16-18737366 site of the No.16 chromosome of soybean, the nucleotide type is T or TC, and the TC type is significantly related to the content of homoleucine. The invention also provides a primer group for detecting the SNP site and a sequence of the primer group, a detection method for PCR amplification, sequencing or enzyme digestion analysis is established based on the primer group, and a matched kit is constructed. The SNP marker can be used for rapid detection of soybean leucine content and molecular marker-assisted breeding of homoleucine varieties, has the advantages of rapid detection, high accuracy, strong applicability and the like, and provides reliable technical support for improvement of soybean nutritional quality.

The application discloses a genetically engineered bacterium with high yield of gallic acid and application thereof, and belongs to the technical field of gene recombination.The genetically engineered bacterium with high yield of gallic acid is prepared from Yarrowia lipolytica ATCC MYA-2613 as a starting strain, and the ku70 gene is knocked out; the gene coding 4-hydroxyphenylpyruvate dioxygenase is expressed; the shikimic acid pathway is strengthened; and three copies of tHMGR and the lysine defect are integrated.In 2L fermenter fed-batch fermentation, the yield of gallic acid is 33.71g / L, which is more than 10 times higher than the highest yield of gallic acid of 2.8g / L of the genetically engineered Yarrowia lipolytica reported at present in 2L fermenter fed-batch fermentation, and the application lays a solid foundation for industrialization of Yarrowia lipolytica for producing gallic acid, and has an industrial application prospect.

Provided herein is a compound having the structure of formula (I), wherein X1 is an acidic β-homo-amino acid or an acidic α-methyl-amino acid; X2 is valine (V) or leucine (L); X3 is citrulline, alanine (A) or lysine (K); A1, A2 and A3 are independently nil or a polyethylene glycol (PEG) moiety comprising 1 to 24 repeats of ethylene glycol (EG) unit; and A3 is nil when X3 is not lysine. Also provided herein are linker units including the present compound of formula (I) for linking effector elements, and antibody-drug conjugates (ADCs) including the present linker units.

The invention relates to the technical field of beer preparation, in particular to high-activity probiotic soybean whey beer and a preparation method thereof, and aims to increase the viable count of probiotics in beer. The preparation method comprises the following steps: fermenting lactic acid bacteria in soybean whey to prepare soybean whey probiotic bacteria base liquid, performing maltolization and boiling wort; and carrying out primary fermentation on the wort, adding the soybean whey and the soybean whey probiotic base solution, and carrying out secondary fermentation to obtain the soybean whey craft beer containing high-activity probiotics. According to the invention, the competitive inhibition effect of saccharomycetes on lactic acid bacteria is reduced through the domestication growth and activity protection effect of the soybean whey on the lactic acid bacteria, so that the activity of probiotics in the beer is fully retained, and the beer has a fermented mellow flavor, also contains rich viable bacteria nutrition and active substances such as leucine, raffinose and stachyose, and has a good taste. The effect of relieving the burdens after drinking is exerted.

The invention discloses application of a soybean GmbZIP104 gene in adaptation of plants to high-salt environmental stress, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the cDNA of the GmbZIP104 gene is as shown in SEQ ID NO. 1; as shown in SEQ ID NO. 2, the amino acid sequence of the GmbZIP104 is shown in SEQ ID NO. The soybean GmbZIP104 gene disclosed by the invention is used for coding a transcription factor consisting of an alkaline region and a leucine zipper structure. The expression quantity is reduced under the condition of soybean salt stress, and by knocking out the GmbZIP104 gene in a soybean body, the symptom represented by the soybean subjected to the salt stress can be relieved; overexpression of the GmbZIP104 gene (GmbZIP104-OE) can cause the salt stress symptom aggravation of soybeans, and the GmbZIP104 gene is a negative regulation salt stress gene. The gene provides a new target gene for breeding a high-salt soybean variety, and provides a theoretical basis for cultivating a high-quality salt-tolerant soybean variety.