The invention relates to a glitter powder sequin membrane and a preparation method thereof. The glitter powder sequin membrane comprises a PET base material layer, a first water glue layer and a glitter powder layer, wherein the water glue of the first water glue layer is obtained by processing the following raw materials in parts by weight: 20-28 parts of ethylene vinyl acetate, 30-45 parts of PU resin copolymer emulsion, 15-23 parts of isooctoate and 15-25 parts of water. The preparation method of the glitter powder sequin membrane comprises the following steps: (I) preparation of glitter powder: selecting a PET base material, aluminizing and coloring, and slitting and powdering to obtain the glitter powder; and (II) preparation of the glitter powder sequin membrane: selecting a PET base material, uniformly coating the PET base material with appropriate water glue, uniformly spreading appropriate glitter powder, and performing extrusion, powder absorption, drying, rolling and slitting processes to obtain the glitter powder sequin membrane. In the invention, oily glue is replaced by waterborne glue; the glitter powder sequin membrane is obtained by gluing, automatic powder spreading, extrusion, powder absorption, protection layer coating and drying; the defect that the oily glue is not environment-friendly is overcome; and the product has the advantages of brightness, beauty, easiness in powdering without tool penetration, boiling resistance, scratch resistance and no falling.