9 results about "Ampicillin Sodium" patented technology

The invention belongs to the technical field of pharmacy, and relates to a method for recovering effective components from ampicillin sodium crystallization mother liquor, which comprises the steps of diisopropylamine recovery, ampicillin trihydrate acid recovery and sodium isooctanoate recovery. According to the method, the diisopropylamine, the ampicillin trihydrate and the sodium isooctanoate are effectively recovered from the solvent-method ampicillin sodium crystallization mother liquor, the recovery rates of the diisopropylamine, the ampicillin trihydrate and the sodium isooctanoate recovered by the method are all higher than 80%, and the quality of the recovered product is good. According to the method, various effective components of the ampicillin sodium crystallization mother liquor are effectively recovered, the production cost can be greatly reduced, meanwhile, the discharge amount of sewage is reduced, and the economic benefits and social benefits of enterprises are improved.

The invention belongs to the technical field of microorganisms, and particularly relates to salt-tolerant and high-temperature-resistant saccharomyces rouxii and application thereof.The method comprises the following steps that S1, screening of salt-tolerant saccharomycetes is conducted, specifically, 25 g of soy sauce mash samples fermented for 30 days, 45 days and 60 days are taken and added into 225 mL of normal saline, and oscillation elution is conducted through a centrifugal machine under the normal-temperature condition; the method comprises the following steps: firstly, performing gradient elution on an eluent, then performing gradient dilution on the eluent to 10 <-3 >, 10 <-4 > and 10 <-5 >, sucking 100 microliters of each gradient diluent, uniformly coating on a YPD agar solid culture medium containing 18 mg / 100 mL of table salt and 100 mg / mL of ampicillin sodium antibiotic, standing and culturing at 30 DEG C, performing repeated separation and purification, and finally selecting a single colony with typical saccharomycetes colony characteristics and maximum diameter; compared with the first soy sauce of the Cantonese-style brewed soy sauce prepared by a normal process, the yeast adding process has the advantages of more glossy color, richer sauce fragrance, ester fragrance, mellow fragrance, more prominent delicate flavor, more palatable salty, fresh and sweet taste, clarification and better wall hanging property.

The invention relates to the technical field of biological medicines, and particularly discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli and a fermentation culture method. The invention discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli. The culture medium comprises a basic culture medium and a fed-batch culture medium, the basic culture medium is prepared from tryptone, a yeast extract, glycerol, a stabilizer, ampicillin sodium, NaCl, K2HPO4, MgSO4 and CaCO3; the solvent is water; the fed-batch culture medium is prepared from glucose, peptone, yeast extract, glycerol, ammonium sulfate, a stabilizer and ampicillin sodium; the solvent is water; the stabilizer is prepared from polydimethylsiloxane aqueous emulsion and palmarosa oil. By utilizing the technical scheme provided by the invention, the fermentation culture time in the fermentation culture method for expressing the FMDV 3ABC antigen by escherichia coli can be shortened, and the obtained antigen product is high in yield and high in purity.

The invention belongs to the field of medicine product crystallization, and discloses a novel ampicillin sodium crystal form and a preparation method thereof. In an X-ray diffraction pattern of the novel crystal form of ampicillin sodium, characteristic peaks exist at 2theta diffraction angles of 6.1 + / -0.2 degrees, 9.4 + / -0.2 degrees, 11.9 + / -0.2 degrees, 12.2 + / -0.2 degrees, 12.7 + / -0.2 degrees, 15.1 + / -0.2 degrees, 16.1 + / -0.2 degrees, 17.6 + / -0.2 degrees, 18.3 + / -0.2 degrees, 18.8 + / -0.2 degrees, 19.7 + / -0.2 degrees, 19.9 + / -0.2 degrees, 21.3 + / -0.2 degrees, 21.5 + / -0.2 degrees, 23.4 + / -0.2 degrees, 24.0 + / -0.2 degrees, 25.2 + / -0.2 degrees, 26.1 + / -0.2 degrees, 27.9 + / -0.2 degrees and 30.2 + / -0.2 degrees. The preparation process of the novel ampicillin sodium crystal form provided by the invention is simple to operate, low in energy consumption, high in economical efficiency and good in reproducibility. The obtained ampicillin sodium new crystal form product is high in crystallinity, good in stability, excellent in morphology, large and uniform in particle size (larger than or equal to 230 microns) and high in content (larger than or equal to 88.0% in terms of C16H19N3O4S, and the sodium content is 5.9%-6.5%).

The application discloses a preparation method of low-moisture ampicillin acid, and belongs to the field of ampicillin acid, and comprises the following steps: step one, stirring and heating after mixing ampicillin trihydrate and isopropyl alcohol in a reaction kettle; step two, starting vacuumizing after heating; step three, carrying out reflux operation after vacuumizing; step four, after refluxing for a certain period of time, leading out distillate, and synchronously supplementing isopropyl alcohol; step five, stopping distillation after leading out distillate, cooling and breaking vacuum; and step six, filtering out solid after cooling, washing, drying and obtaining low-moisture ampicillin acid. The water and isopropyl alcohol of ampicillin trihydrate are slowly distilled out of the system by azeotropic distillation, so that low-moisture ampicillin acid is obtained. The low-moisture ampicillin acid can slow down the degradation of ampicillin by water, effectively reduce the impurity content, and improve the yield of ampicillin sodium produced by ampicillin acid.

The present invention discloses an ampicillin sodium and sulbactam sodium pharmaceutical composition for injection and a preparation method thereof. The preparation method includes Step 1: uniformly mixing ampicillin sodium and sulbactam sodium; Step 2: uniformly mixing water-soluble chitosan microspheres with the mixture obtained in Step 1; Step 3: uniformly mixing sodium carboxymethyl cellulose with the mixture obtained in Step 2. In the present invention, by mixing water-soluble chitosan microspheres with ampicillin sodium and sulbactam sodium, the water-soluble chitosan microspheres can be uniformly dispersed in ampicillin sodium and sulbactam sodium. Then, the added sodium carboxymethyl cellulose forms a hydrogen bond with the water-soluble chitosan microspheres, so that a film is formed on the outside of the water-soluble chitosan microspheres by sodium carboxymethyl cellulose, and ampicillin sodium and sulbactam sodium can be dispersed in the dispersion system formed by sodium carboxymethyl cellulose and water-soluble chitosan microspheres, thereby providing long-term and stable protection for ampicillin sodium and sulbactam sodium.

The invention discloses a comprehensive recovery method of ampicillin sodium crystallization mother liquor, which comprises the following steps: enabling the ampicillin sodium crystallization mother liquor to pass through oxyhydrogen anion exchange resin, adsorbing ampicillin acid radical ions and isooctanoic acid radical ions on the resin through ion exchange, the ampicillin hydrochloride and the isocaprylic acid desorption solution obtained through desorption are separated through macroporous adsorption resin, and then the ampicillin and the isocaprylic acid salt are obtained through the processes of desorption, crystallization and drying. According to the method, the oxyhydrogen anion exchange resin and the macroporous adsorption resin are combined to separate and recover the active ingredients ampicillin and isocaprylic acid in the mother liquor, and the ion exchange and adsorption process is mild in condition, can be carried out at normal temperature, and has high recovery purity and recovery rate; and the cost and difficulty of the subsequent refining process are reduced.

The invention belongs to the technical field of pharmacy, and relates to a preparation method of ampicillin sodium. The preparation method comprises the following steps: mixing ampicillin acid and sodium type cation exchange resin in water according to a certain proportion, stirring and dissolving, filtering to obtain an ampicillin sodium solution, and concentrating and freeze-drying to obtain the ampicillin sodium with the quality meeting the standard. According to the preparation method, the raw material ampicillin acid does not need to be dehydrated, any organic solvent does not need to be used, the ampicillin acid can be directly converted into the ampicillin sodium only through the cation exchange resin, the process design is innovative, the operation is simple and convenient, the energy consumption is low, and the obtained ampicillin sodium product is high in quality and stable.

The invention provides a human mycoplasma pneumoniae culture medium, a mycoplasma culture method and application, and relates to the technical field of microbial materials. The human mycoplasma pneumoniae culture medium comprises a basic culture medium, fetal calf serum, ampicillin sodium and an additive, and the additive comprises the following components based on the total volume of the human mycoplasma pneumoniae culture medium: 0.8-1.6 mg / L of glutamic acid, 0.4-1.2 mg / L of cysteine, 0.6-1.4 mg / L of glutathione, 50-150 mM / L of sodium pyruvate and 50-200 mL / L of a yeast extract. And the pH value of the human mycoplasma pneumoniae culture medium after sterilization is 7.6-8.0. According to the invention, the growth and reproduction speed of the mycoplasma pneumoniae is accelerated through the synergistic effect of the specific additive composition and content and the whole culture medium, a large number of mycoplasma pneumoniae wet thalli can be obtained in a short time, the survival rate of the human mycoplasma pneumoniae is obviously improved, and the method can be widely applied to large-scale production of the human mycoplasma pneumoniae natural antigen.