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38 results about "Penicillin binding" patented technology
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Interacting selectively and non-covalently with penicillin, any antibiotic that contains the condensed beta-lactamthiazolidine ring system. [GOC:ai]
Kit for detecting beta-lactam antibiotic ligand in milk by receptor method and detection method thereof
ActiveCN101813698AShort detection timeHigh detection throughputDepsipeptidesTesting dairy productsPenicillin bindingDiluent
The invention discloses a kit for detecting beta-lactam antibiotic ligand in milk by a receptor method and a detection method thereof. The kit comprises the following components: (1) an enzyme-labeled plate which is coated by penicillin-binding protein PBP2xa or PBP2xb recombinant protein; (2) an enzyme-labeled marker; (3) standard solution of ampicillin sodium; (4) 20 times concentrated cleaning buffer solution; (5) enzyme-labeled diluent; (6) 20 times concentrated sample extract; (7) developing solution; and (8) reaction stop solution. The kit of the invention can simultaneously screen eight beta-lactam antibiotic residues in the milk; the detection limit is lower than the minimum residue limit of China and main developed countries in the world; the detection time is within 50 minutes; and the kit has the characteristics of simple, rapid and accurate operation, low cost and the like and is suitable for large-scale popularization and application.
Owner:上海溯源生物技术有限公司
Method, special kit and test paper strip for detecting beta-lactam antibiotics based on penicillin-binding protein
ActiveCN102253216AHigh sensitivityImprove accuracyBiological testingAmpicillinBeta lactam antibiotic
The invention discloses a method, a special kit and test paper strips for detecting beta-lactam antibiotics based on penicillin-binding protein. The receptor kit used for detecting beta-lactam antibiotics comprises: protein represented by SEQ ID NO: 1, enzyme labeled ampicillin and a standard substance solution, wherein the standard substance is penicillin G. The kit and colloidal gold test paper strips provided by the invention have advantages of high sensitivity, high accuracy, high precision, low cost, simple operation, short detection period, simple storage, and long shelf-life. The kit and colloidal gold test paper strips are suitable for various work units. With the kit and colloidal gold test paper strips, simultaneous and rapid detections of large batches of samples can be realized, and on-site high flux rapid detections can be realized. Therefore, the kit, the colloidal gold test paper strips and the method provided by the invention play an important role in the detections of beta-lactam antibiotics.
Owner:北京维德维康生物技术有限公司 +1
Therapeutic treatment for sexual dysfunction
InactiveUS7166626B2Improve sexual functionNormal and enhanced sexual functionBiocideAnimal repellantsSexual functionPenicillin binding
A method for improving sexual function is described. A mammal suffering from sexual dysfunction or otherwise in need of enhanced sexual function is administered a compound selected from those that are capable of inhibiting the activity of β-lactams, penicillin-binding protein, carboxypeptidase,. Such compounds, including particularly β-lactam ring-containing compounds, can be used to formulate pharmaceutical formulations useful for improving sexual function.
Owner:REVAAX PHARMA LLC
Method for preparing detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay and detection method thereof
ActiveCN104215775AHigh sensitivityHigh detection sensitivityBiological testingAntibiotic YMacrolide resistance
The invention aims to provide a method for preparing a rapid detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay as well as a used detection method, aiming at solving the problem of sieve leakage in the process of detecting beta-lactam medicines in a traditional ELISA method. The invention discloses a method for preparing a detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay as well as an indirect competitive receptor assay method for detecting the content of beta-lactam drugs in milk by adopting the detection kit based on penicillin-binding protein PBP6 beta-lactam antibiotic receptor assay. The methods have the beneficial effects that by utilizing the characteristic that the penicillin-binding protein 6 can be specifically bound to the beta-lactam drugs, the drugs can be specifically identified in a buffer liquid system or a sample matrix extracting solution and are not influenced by other antibiotics such as macrolides, quinolones and tetracyclines.
Owner:北京纳百生物科技有限公司
Method for preparing penicillin binding protein combined with beta-lactam antibiotic and special recombinant bacterium
InactiveCN102181394AHigh yieldBacteriaMicroorganism based processesPenicillin bindingRecombinant escherichia coli
The invention discloses a method for preparing a penicillin binding protein combined with beta-lactam antibiotic and a special recombinant bacterium. The recombinant bacterium is prepared by the following steps of: importing the coding gene of the penicillin binding protein into initial escherichia coli to obtain recombinant escherichia coli. Experiments prove that the method for preparing the penicillin binding protein has high yield, and the problems of low yield and difficulty in preparation in the prior art are solved. Therefore, the method has significance.
Owner:CHINA AGRI UNIV
Carbapenem compounds
A compound or its pharmaceutically acceptable salt represented by the following formula:The invention is a carbapenem compound which has a potent antibacterial activity over a broad range of Gram negative and Gram positive bacteria, especially penicillin-resistant Streptococcus pneumoniae (PRSP) which has been isolated at an elevated frequency in recent years and thus causes a serious clinical problem, and Haemophilus influenzae which has acquired resistance against the existing β-lactam antibiotics over a wide scope due to penicillin-binding protein (PBP) mutations such as β-lactamase non-producing ampicillin-resistant (BLNAR) Haemophilus influenzae, and has excellent oral absorbability.
Owner:SUMITOMO DAINIPPON PHARMA CO LTD
High-affinity penicillin binding protein and application thereof
InactiveCN102627693ABacteriaMicroorganism based processesStreptococcus agalactiaeBeta lactam antibiotic
The invention provides a high-affinity penicillin binding protein. The invention is characterized in that the high-affinity penicillin binding protein is obtained by respectively transforming isoleucine 526 and glutamine 547 in a streptococcus agalactiae penicillin binding protein (PBP 1b) into lysine and arginine, so that the affinity of beta-lactam antibiotics is improved. The invention furtherprovides a quick detection test strip produced based on the protein, which can be used for quickly detecting beta-lactam antibiotic residues in agricultural and sideline products.
Owner:PINGYUAN COUNTY WEIFENG YONGZHU TECH
Neurotherapeutic treatment for sexual dysfunction
InactiveUS20070093466A1Normal and enhanced sexual functionSufficient blood-brain barrier transport propertyBiocideAnimal repellantsSexual functionPenicillin binding
Owner:REVAAX PHARMA LLC
Molecular modified penicillin-binding protein (PBP) capable of detecting beta-lactam antibiotic residues
The invention provides a molecular modified penicillin-binding protein (PBP) capable of detecting beta-lactam antibiotic residues. The molecular modified PBP is characterized by being obtained by respectively modifying leucine on the 147th position of the PBP of actinomadura yumaense R39 and histidine on the 351st position of the PBP of the actinomadura yumaense R39 into histidine and aspartic acid, so that the affinity of beta-lactam antibiotics is improved. The invention also provides a rapid detecting test strip based on production of the protein, and the rapid detecting test strip can be used for rapidly detecting the beta-lactam antibiotic residues of agricultural and sideline products.
Owner:JILIN ACAD OF AGRI SCI
Novel carbapenem compounds
InactiveUS20050020566A1High antibacterial activityPromote oral absorptionAntibacterial agentsBiocideAcquired resistanceAmpicillin
A compound or its pharmaceutically acceptable salt represented by the following formula: The invention is a carbapenem compound which has a potent antibacterial activity over a broad range of Gram negative and Gram positive bacteria, especially penicillin-resistant Streptococcus pneumoniae (PRSP) which has been isolated at an elevated frequency in recent years and thus causes a serious clinical problem, and Haemophilus influenzae which has acquired resistance against the existing β-lactam antibiotics over a wide scope due to penicillin-binding protein (PBP) mutations such as β-lactamase non-producing ampicillin-resistant (BLNAR) Haemophilus influenzae, and has excellent oral absorbability.
Owner:SUMITOMO DAINIPPON PHARMA CO LTD
Enzyme-linked immunoassay kit for detecting methicillin-resistant staphylococcus aureus
ActiveCN105315367AGrowth inhibitionEasy to operateAntibacterial agentsImmunoglobulins against bacteriaElisa kitPenicillin binding
The invention discloses a monoclonal antibody against a penicillin-binding protein PBP2[alpha], wherein the monoclonal antibody is secreted from a hybridoma cell strain PBP2[alpha]-4B8-G7-H7 with an accession number of CCTCC C2014128. The invention further discloses an enzyme-linked immunoassay kit for detecting methicillin-resistant staphylococcus aureus. A coating antibody is the anti-PBP2[alpha] monoclonal antibody disclosed by the invention. A detection antibody is an HRP labeled PBP2[alpha] polyclonal antibody. The kit disclosed by the invention can be used for detecting methicillin-resistant staphylococcus aureus in a detection sample through the anti-PBP2[alpha] monoclonal antibody and has the characteristics of being simple and convenient, rapid, practical, sensitive and efficient.
Owner:RAYBIOTECH INC GUANGZHOU
Method for secretory production of protein
A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that activity of a penicillin-binding protein is reduced and in which activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.
Owner:AJINOMOTO CO INC
Method for efficiently synthesizing mequinomycin A in streptomyces albus
ActiveCN112410278AImprove toleranceIncrease productionBacteriaMicroorganism based processesHeterologousMutant
The invention discloses a method for efficiently synthesizing mequinomycin A in streptomyces albus. The invention is based on the method of introducing site-directed mutant A penicillin binding protein gene into the heterologous expression host Streptomyces.albus of the mequinomycin A biosynthesis gene cluster to optimize the heterologous host, and the mequinomycin A acts on peptidoglycan transferase structural domain active sites of bacterial cell walls aPBP to effectively inhibit the synthesis of the cell walls of Gram-positive bacteria. Large-fragment assembly and promoter optimization arecarried out on the mequinomycin biosynthesis gene cluster by means of genetic engineering, and the mequinomycin A resistance gene MaPBP is introduced into the optimized strain II to further improve the mequinomycin resistance of the strain II. Finally, the heterologous expression yield of the mequinomycin A in the strain LX03 is increased by 110% compared with that of the original strain I. According to the invention, not only the genetic engineering modification method of large-segment gene clusters is described, but also a precedent that a Streptomyces. Albus host is optimized by a resistance gene to improve the yield of a target compound is started.
Owner:SHANGHAI JIAO TONG UNIV
Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus
ActiveUS20120009572A1Easy to markMicrobiological testing/measurementStaphylococcus cohniiCommunity associated
The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and / or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.
Owner:MEDICAL DIAGNOSTIC LAB
Single nucleotide polymorphisms and community-associated methicillin-resistant Staphylococcus aureus
The present invention is based on the discovery of polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and / or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.
Owner:MEDICAL DIAGNOSTIC LAB
Penicillin-binding protein and application thereof
ActiveCN106591265AEasy to handleLow skill level requiredBacteriaMicrobiological testing/measurementBiotechnologyPenicillin binding
The invention provides a novel penicillin-binding protein Bt-PBP3 and encoding genes thereof. The protein contains an amino acid sequence shown in SEQ ID No.2 or the protein is obtained by substitution, deletion and / or addition of one or more amino acids on the amino acid sequence shown in the SEQ ID No.2 and has the equal activity. The protein can be used for preparing a penicillin detection reagent, and used for penicillin detection, no expensive instrument equipment is needed, the sample pretreatment process is simple, and the requirement for the technological level of analysis personnel is low, and the protein is suitable for rapid on-site detection.
Owner:WUHAN POLYTECHNIC
Monoclonal antibody against pbp2a derived from mrsa with dual binding activities
Mouse monoclonal antibodies specifically recognizing the Penicillin Binding Protein 2a (PBP2a) derived from a strain of Methicillin-Resistant Staphylococcus aureus (MRSA) were produced and characterized. The immunogen used to generate an immune response in a mouse was a PBP2a recombinant protein derived from a strain of Methicillin-Resistant Staphylococcus aureus (MRSA). The data showed that both monoclonal antibodies of the disclosure were able to distinguish MRSA from MSSA bacteria. The monoclonal antibodies have distinct recognition patterns for the regions of the PBP2a protein sequence. Epitope mapping has localized regions of the PBP2a protein specifically recognized by one or both of the monoclonal antibodies. The monoclonal antibodies of the present disclosure having the ability to distinguish between MRSA and MSSA strains can be useful as the basis for a diagnostic assay useful in the clinical setting for determining whether and which antibiotics to administer to a patient.
Owner:RAYBIOTECH INC GUANGZHOU
Quinazolinone antibiotics
ActiveUS9776975B2High in vitro potencyHigh activityAntibacterial agentsOrganic chemistryStaphylococcusPenicillin binding
Owner:UNIV OF NOTRE DAME DU LAC
Preparation and New Application of Penicillin Binding Protein Antibacterial Agent
ActiveCN105238830BImprove compatibilityGood development resourceBiocideMicroorganism based processesEcological environmentAnion-exchange chromatography
The present invention discloses a penicillin binding protein extract and novel application thereof. The antibacterial function of the protein has not been reported yet. The penicillin binding protein extract passes through a DEAE-cellulose anion exchange chromatography to obtain a high purity penicillin binding protein; and a flat coating punching method is used for determination of the influence of penicillin binding protein in different concentrations on Monilinia fructicola. Through antibacterial spectrum determination, the protein has the advantages of broad-spectrum antibacterial property, strong antibacterial activity, safety to mammals, no cause of pesticide injury, toxicity residue, safety for people, livestock, and ecological environment. At the same time, the protein is conducive to industrial production, and provides a scientific basis for biological control of agricultural pest.
Owner:BEIJING GUANGHE BIOTECH CO LTD
Quinazolinone antibiotics
ActiveUS20160031832A1High in vitro potencyHigh activityAntibacterial agentsBiocideBacteroidesStaphylococcus cohnii
A new class of antibiotics effective against methicillin-resistant Staphylococcus aureus (MRSA) is disclosed. Compounds of this class can impair cell-wall biosynthesis by binding to both the allosteric and the catalytic domains of penicillin-binding protein (PBP) 2a. This class of antibiotics holds promise in reversing obsolescence of staphylococcal PBPs as important targets for antibiotics. Embodiments of the invention thus provide novel antibacterial compounds that target penicillin-binding proteins and / or other important cellular targets. Methods for inhibiting the growth and / or replication of bacteria using the compounds described herein are also provided. Various embodiments exhibit activity against gram positive bacteria, including drug-resistant strains of Staphylococcus aureus.
Owner:UNIV OF NOTRE DAME DU LAC
Kit for detecting beta-lactam antibiotic ligand in milk by receptor method and detection method thereof
ActiveCN101813698BLow detection limitHigh sensitivityTesting dairy productsDepsipeptidesPenicillin bindingDiluent
Owner:上海溯源生物技术有限公司
Immunochromatography test strip for detecting carbapenemase in bacteria and detection method
ActiveCN114487404ABiological material analysisAgainst vector-borne diseasesReceptorPenicillin binding
Owner:CHINA AGRI UNIV
A kind of penicillin binding protein and its application
ActiveCN106591265BEasy to handleLow skill level requiredBacteriaMicrobiological testing/measurementPenicillin bindingPenicillin K
The invention provides a novel penicillin-binding protein Bt-PBP3 and encoding genes thereof. The protein contains an amino acid sequence shown in SEQ ID No.2 or the protein is obtained by substitution, deletion and / or addition of one or more amino acids on the amino acid sequence shown in the SEQ ID No.2 and has the equal activity. The protein can be used for preparing a penicillin detection reagent, and used for penicillin detection, no expensive instrument equipment is needed, the sample pretreatment process is simple, and the requirement for the technological level of analysis personnel is low, and the protein is suitable for rapid on-site detection.
Owner:WUHAN POLYTECHNIC
Detection card for rapidly and quantitatively detecting penicillin and application thereof
PendingCN110579606AQuick checkQuantitative detectionBiological testingFluorescencePenicillin binding
The embodiment of the invention discloses a detection card for rapidly and quantitatively detecting penicillin and an application thereof. The detection card comprises a bottom plate, a sample pad, acombination pad, a base membrane and a water absorption pad, and is characterized in that the combination pad contains quantum dot labeled penicillin binding protein. The detection card disclosed by the invention uses quantum dots to label penicillin binding protein, so that the sensitivity of fluorescence detection can be greatly improved. Besides, the detection card can be used for rapidly, sensitively and quantitatively detecting penicillin, a result can be rapidly obtained by adopting a quantum dot quantitative reading instrument, the detection time is greatly shortened, and convenience isprovided for field detection.
Owner:武玉香
Penicillin binding protein from Streptococcus pneumoniae
The invention provides isolated nucleic acid compounds encoding a novel high molecular weight PBP of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the PBP and a method for identifying compounds that bind and / or inhibit the enzymatic activity of the PBP.
Owner:ELI LILLY & CO
Preparation and application of a penicillin-tremorin binding antigen and its antibody
ActiveCN106526167BHigh potencyIncreased sensitivityOvalbuminSerum albuminElisa kitCyclopiazonic acid
The invention discloses a penitrem A combined antigen as well as preparation and an application of an antibody of the antigen. The median inhibitory dose (IC50), detected by a penitrem A enzyme-linked immunosorbent assay kit, of penitrem A for a reaction between a specific antibody of the penitrem A and penitrem A coating antigens is 100 ng / mL, the lowest detection limit of penitrem A is 30 ng / mL, and the cross reaction rate of penitrem A and an aflatoxin B1 sample, the cross reaction rate of penitrem A and a cyclopiazonic acid sample, the cross reaction rate of penitrem A and a vomitoxin and the cross reaction rate of penitrem A and a citreoviridin sample are all smaller than 0.1%. The specific antibody of penitrem A in the penitrem A enzyme-linked immunosorbent assay kit has the characteristics of high potency, high sensitivity and high specificity and can be used for quickly, sensitively and easily detecting the residual amount of penitrem A in animal-origin food.
Owner:CHINA AGRI UNIV
Penicillin-binding protein bt-pbp2x and its application
ActiveCN106085982BEasy to handleLowering techniqueBacteriaMicrobiological testing/measurementPenicillin bindingPenicillin K
Owner:岩璟生物科技武汉有限公司
Penicillin-binding protein Bt-pbp2X and application thereof
ActiveCN106085982AEasy to handleLowering techniqueBacteriaMicrobiological testing/measurementPenicillin bindingPenicillin K
The invention provides a novel penicillin-binding protein Bt-pbp2X and encoding genes thereof. The protein has an amino acid sequence shown in SEQ ID No.2, or has the amino acid sequence obtained through substitution, deletion and / or increase of one or more amino acids from the amino acid sequence shown in SEQ ID No.2 with equal activity. The protein can be used for preparing a penicillin antibiotic detection reagent and can be applied to rapid detection of penicillin antibiotic residues. A complex instrument is not needed, the sample pretreatment process is very simple, specific requirements are made for technological and knowledge level of detection people, and the protein is very suitable for spot rapid detection.
Owner:岩璟生物科技武汉有限公司
Enzyme-linked immunosorbent assay kit for the detection of methicillin-resistant Staphylococcus aureus
ActiveCN105315367BGrowth inhibitionEasy to operateAntibacterial agentsImmunoglobulins against bacteriaElisa kitPenicillin binding
The invention discloses a monoclonal antibody against a penicillin-binding protein PBP2[alpha], wherein the monoclonal antibody is secreted from a hybridoma cell strain PBP2[alpha]-4B8-G7-H7 with an accession number of CCTCC C2014128. The invention further discloses an enzyme-linked immunoassay kit for detecting methicillin-resistant staphylococcus aureus. A coating antibody is the anti-PBP2[alpha] monoclonal antibody disclosed by the invention. A detection antibody is an HRP labeled PBP2[alpha] polyclonal antibody. The kit disclosed by the invention can be used for detecting methicillin-resistant staphylococcus aureus in a detection sample through the anti-PBP2[alpha] monoclonal antibody and has the characteristics of being simple and convenient, rapid, practical, sensitive and efficient.
Owner:RAYBIOTECH INC GUANGZHOU
Method for improving expression level of bacillus subtilis recombinant protein based on low PAM-dependent CRISPR regulation
The invention discloses a method for improving the expression level of bacillus subtilis recombinant protein based on low PAM (Polyacrylamide)-dependent CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) regulation, and belongs to the field of gene engineering. According to the invention, the CRISPRa / i system VcaQCas-RpoZ capable of mediating gene expression activation and inhibition regulation in bacillus subtilis at the same time is obtained by adding an activation module RpoZ. After the system is integrated and expressed into a bacillus subtilis genome, transcription of chaperonin PrsA and penicillin binding protein PonA is activated, meanwhile, the transcriptional level of a regulatory factor MurR is inhibited, and the expression level of recombinant protein is remarkably improved. The enzyme activity of the ultrahigh-temperature amylase is improved to 2.46 times of that of a control strain. The bacillus subtilis provided by the invention provides a new gene expression regulation strategy as a foreign protein expression host, and also provides reference for site selection of gene regulation.
Owner:JIANGNAN UNIV