35 results about "Penicillin binding" patented technology

The invention discloses a method, a special kit and test paper strips for detecting beta-lactam antibiotics based on penicillin-binding protein. The receptor kit used for detecting beta-lactam antibiotics comprises: protein represented by SEQ ID NO: 1, enzyme labeled ampicillin and a standard substance solution, wherein the standard substance is penicillin G. The kit and colloidal gold test paper strips provided by the invention have advantages of high sensitivity, high accuracy, high precision, low cost, simple operation, short detection period, simple storage, and long shelf-life. The kit and colloidal gold test paper strips are suitable for various work units. With the kit and colloidal gold test paper strips, simultaneous and rapid detections of large batches of samples can be realized, and on-site high flux rapid detections can be realized. Therefore, the kit, the colloidal gold test paper strips and the method provided by the invention play an important role in the detections of beta-lactam antibiotics.

A method for improving sexual function is described. A mammal suffering from sexual dysfunction or otherwise in need of enhanced sexual function is administered a compound selected from those that are capable of inhibiting the activity of β-lactams, penicillin-binding protein, carboxypeptidase,. Such compounds, including particularly β-lactam ring-containing compounds, can be used to formulate pharmaceutical formulations useful for improving sexual function.

The invention provides a high-affinity penicillin binding protein. The invention is characterized in that the high-affinity penicillin binding protein is obtained by respectively transforming isoleucine 526 and glutamine 547 in a streptococcus agalactiae penicillin binding protein (PBP 1b) into lysine and arginine, so that the affinity of beta-lactam antibiotics is improved. The invention furtherprovides a quick detection test strip produced based on the protein, which can be used for quickly detecting beta-lactam antibiotic residues in agricultural and sideline products.

The invention provides a molecular modified penicillin-binding protein (PBP) capable of detecting beta-lactam antibiotic residues. The molecular modified PBP is characterized by being obtained by respectively modifying leucine on the 147th position of the PBP of actinomadura yumaense R39 and histidine on the 351st position of the PBP of the actinomadura yumaense R39 into histidine and aspartic acid, so that the affinity of beta-lactam antibiotics is improved. The invention also provides a rapid detecting test strip based on production of the protein, and the rapid detecting test strip can be used for rapidly detecting the beta-lactam antibiotic residues of agricultural and sideline products.

A compound or its pharmaceutically acceptable salt represented by the following formula:

The invention is a carbapenem compound which has a potent antibacterial activity over a broad range of Gram negative and Gram positive bacteria, especially penicillin-resistant Streptococcus pneumoniae (PRSP) which has been isolated at an elevated frequency in recent years and thus causes a serious clinical problem, and Haemophilus influenzae which has acquired resistance against the existing β-lactam antibiotics over a wide scope due to penicillin-binding protein (PBP) mutations such as β-lactamase non-producing ampicillin-resistant (BLNAR) Haemophilus influenzae, and has excellent oral absorbability.

The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.

The invention provides a novel penicillin-binding protein Bt-PBP3 and encoding genes thereof. The protein contains an amino acid sequence shown in SEQ ID No.2 or the protein is obtained by substitution, deletion and/or addition of one or more amino acids on the amino acid sequence shown in the SEQ ID No.2 and has the equal activity. The protein can be used for preparing a penicillin detection reagent, and used for penicillin detection, no expensive instrument equipment is needed, the sample pretreatment process is simple, and the requirement for the technological level of analysis personnel is low, and the protein is suitable for rapid on-site detection.

A new class of antibiotics effective against methicillin-resistant Staphylococcus aureus (MRSA) is disclosed. Compounds of this class can impair cell-wall biosynthesis by binding to both the allosteric and the catalytic domains of penicillin-binding protein (PBP) 2a. This class of antibiotics holds promise in reversing obsolescence of staphylococcal PBPs as important targets for antibiotics. Embodiments of the invention thus provide novel antibacterial compounds that target penicillin-binding proteins and/or other important cellular targets. Methods for inhibiting the growth and/or replication of bacteria using the compounds described herein are also provided. Various embodiments exhibit activity against gram positive bacteria, including drug-resistant strains of Staphylococcus aureus.

The invention discloses an immunochromatography test strip for detecting carbapenemase in bacteria and a detection method. The immunochromatography test strip for detecting carbapenemase in bacteria comprises a sample pad, an NC film, a water absorption pad and a bottom plate which are sequentially pasted on the bottom plate, the NC film is provided with a detection line and a quality control line, the detection line is coated with penicillin-BSA, and the quality control line is coated with a His fusion tag antibody. The invention also provides a kit for detecting carbapenemase in bacteria. The kit comprises a bacterial lysis solution, reaction holes I and II and the test strip, the reaction hole I is coated with meropenem, and the reaction hole II is coated with a PBP 4'receptor protein compound marked by colloidal gold. According to the method, meropenem with stable properties is selected as a substrate, gold-labeled penicillin binding protein PBP 4'is taken as a receptor, and whether carbapenemase exists in a sample or not is indirectly detected by detecting the consumption condition of the substrate, so that a powerful technical means is provided for rapid detection of carbapenemase in bacteria.

The embodiment of the invention discloses a detection card for rapidly and quantitatively detecting penicillin and an application thereof. The detection card comprises a bottom plate, a sample pad, acombination pad, a base membrane and a water absorption pad, and is characterized in that the combination pad contains quantum dot labeled penicillin binding protein. The detection card disclosed by the invention uses quantum dots to label penicillin binding protein, so that the sensitivity of fluorescence detection can be greatly improved. Besides, the detection card can be used for rapidly, sensitively and quantitatively detecting penicillin, a result can be rapidly obtained by adopting a quantum dot quantitative reading instrument, the detection time is greatly shortened, and convenience isprovided for field detection.

The invention provides a novel penicillin-binding protein Bt-pbp2X and encoding genes thereof. The protein has an amino acid sequence shown in SEQ ID No.2, or has the amino acid sequence obtained through substitution, deletion and/or increase of one or more amino acids from the amino acid sequence shown in SEQ ID No.2 with equal activity. The protein can be used for preparing a penicillin antibiotic detection reagent and can be applied to rapid detection of penicillin antibiotic residues. A complex instrument is not needed, the sample pretreatment process is very simple, specific requirements are made for technological and knowledge level of detection people, and the protein is very suitable for spot rapid detection.

The invention provides isolated nucleic acid compounds encoding a novel high molecular weight PBP of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the PBP and a method for identifying compounds that bind and/or inhibit the enzymatic activity of the PBP.