Immunochromatography test strip for detecting carbapenemase in bacteria and detection method

Abstract

The invention discloses an immunochromatography test strip for detecting carbapenemase in bacteria and a detection method. The immunochromatography test strip for detecting carbapenemase in bacteria comprises a sample pad, an NC film, a water absorption pad and a bottom plate which are sequentially pasted on the bottom plate, the NC film is provided with a detection line and a quality control line, the detection line is coated with penicillin-BSA, and the quality control line is coated with a His fusion tag antibody. The invention also provides a kit for detecting carbapenemase in bacteria. The kit comprises a bacterial lysis solution, reaction holes I and II and the test strip, the reaction hole I is coated with meropenem, and the reaction hole II is coated with a PBP 4'receptor protein compound marked by colloidal gold. According to the method, meropenem with stable properties is selected as a substrate, gold-labeled penicillin binding protein PBP 4'is taken as a receptor, and whether carbapenemase exists in a sample or not is indirectly detected by detecting the consumption condition of the substrate, so that a powerful technical means is provided for rapid detection of carbapenemase in bacteria.

Description

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AI Technical Summary

Benefits of technology

This technology uses an enzyme called Penicilast that binds specifically to certain types of beta lactam antibiotics (carbaoxyphenylpenoid). By measuring these interactions between them, it allows us to determine whether there are any specific chemical structures associated with those substances. These chemistry indicate how effective they fight against drug resistant microorganisms such as Escherichia coli.

Problems solved by technology

This patents discusses how different types of drug resistant microorganisms may produce similar substances called beta lactam compounds or other chemical structures known collectively as metabolites. These chemistries cause cross talk between certain organism cells when exposed to specific agents like those used against infectious diseases. To diagnose this problem, it becomes necessary to identify any changes made due to their presence. Current techniques involve genetic tests involving DNA polymerization technology, immunological assays, histochemistry analysis, etc., making diagnosis difficult at times unpredictable because they require trained professionals who often lack advanced technical expertise.

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