46 results about "Bacterial lysate" patented technology

The invention discloses a kit for extracting nucleic acid from bacteria by using a paramagnetic particle method and an extracting method. The kit comprises a bacteria lysate, a magnetic bead binding solution, a magnetic bead scrubbing solution and a nucleic acid eluent. The bacteria lysate comprises sodium dodecyl sulfate, ethylenediaminetetraacetic acid, tri-hydroxymethyl aminomethane and sodium chloride; the magnetic bead binding solution comprises polyethylene glycol-8000 and sodium chloride; the magnetic bead scrubbing solution comprises ethanol; and the nucleic acid eluent comprises tri-hydroxymethyl aminomethane and ethylenediamine tetraacetic acid. The kit comprises the unique bacteria lysate which has a strong lysis function for the bacteria; and the kit can be used for manual extraction and instrument extraction by using a commercially available nucleic acid isolation machine, high-purity and high-concentration bacteria nucleic acid can be extracted.

Disclosed herein are compositions and methods for regulating redox status and/or reducing oxidative stress in a subject, the methods and compositions comprising TLR agonists comprising bacterial lysates and/or lysate fractions. Also disclosed are compositions and methods comprising bacterial lysates and/or lysate fractions formulated or administered in combination with one or more other therapeutic or pharmaceutical agents.

The invention relates to a flocculant production method, in particular to a production method for microbial flocculants, and aims to solve the problems of long production cycle of existing flocculant production methods and poor flocculation effect of flocculants. The method includes 1, picking acinetobacter and inoculating the same into a rich culture medium to obtain an active bacterium solution, and inoculating the bacterium solution into a flocculation culture medium; 2, performing fermentation cultivation to obtain a fermentation broth; 3, centrifuging the fermentation broth to collect thallus sediments, adding a bacteria lysate into the sediments, performing ultrasonic disruption, collecting a supernatant through centrifugation, adding CTAB (cetyltrimethyl ammonium bromide), collecting sediments after standing and centrifuging, adding absolute ethyl alcohol and acetone, collecting a supernatant through centrifugation, adding ethyl alcohol, collecting sediments through centrifugation, and washing the sediments which are the microbial flocculants. The production method applied to the field of waste water treatment is short in production cycle, and the flocculation effect of the microbial flocculants is good.

The invention relates to a double-magnetic-bead method based centrifugation-free extraction kit for plasmid DNA (deoxyribonucleic acid). The kit accommodates an impurity-removing magnetic bead suspension liquid, an extraction magnetic bead suspension liquid, a bacterium lysis solution, a binding buffer solution, a cleaning solution and an eluent which are separately subpackaged, wherein impurity-removing magnetic beads are dispersed in the impurity-removing magnetic bead suspension liquid and surfaces of the impurity-removing magnetic beads are at least modified with styrene, divinyl benzene and iminodiacetic acid; extraction magnetic beads are dispersed in the extraction magnetic bead suspension liquid and surfaces of the extraction magnetic beads are modified with silicon hydroxyl. According to development of the impurity-removing magnetic beads, impurities such as cell walls, protein and the like are removed after bacterium lysis, the plasmid DNA in a liquid supernatant is reserved, so that the centrifugation step in a traditional process is replaced, and the operation is more efficient and faster; when the kit is in use, the buffer solution, the magnetic bead suspension liquid and the like can be subpackaged in corresponding pore plates of a nucleic acid extraction instrument, and requirements for automation and high throughput are met; the extracted plasmid DNA has high purity and complete fragments.

The invention discloses a method for extracting bacterium genomic DNA by using magnetic nanoparticles, belonging to the technical field of nanomaterials and molecular biology. The method comprises the steps of: preparing a magnetic nanoparticle aggregate, preparing a bacterium lysing solution, preparing and adding an absorption buffer solution, absorbing with a magnet, rinsing with 70 percent alcohol, and eluting with a TE buffer solution to obtain a bacterium genomic DNA solution. Compared with the traditional phenol/chloroform DNA extracting method, the method for extracting the bacterium genomic DNA by using magnetic nanoparticles has the advantages of short experiment time, simpleness in operation, high extraction efficiency, rapidness, convenience, sensitivity, high yield and the like; and the extracted bacterium genomic DNA can be completely used for subsequent experiments, such as DNA hybridization, PCR (Polymerase Chain Reaction), and the like, and provides the basis for the subsequent research and analysis.

The invention provides a reagent and a method for extracting plasmids. The reagent for extracting plasmids comprises a bacteria lysis solution, an endotoxin removing grain solution, an endotoxin removing solution, a plasmid combining solution, a cleaning solution, an eluant and the like. According to the reagent and the method for extracting the plasmids provided by the invention, the operation steps are simple, the time for extracting is short, the endotoxin content in the extracted plasmids can be effectively reduced, the content of endotoxin in the extracted plasmids can be reduced to be lower than 0.1EU/mu g to satisfy the clinical criteria and the obtained plasmids have high DNA (Deoxyribonucleic Acid) yield and good purity.

The invention discloses an ELISA (Enzyme-linked Immuno Sorbent Assay) antigen detection kit of animal echinococcosis granulosa. The detection kit is prepared from an anti-EgAgB8/2 protein monoclonal antibody pre-coated ELISA plate, a sample diluting solution, a positive control solution, a negative control solution, an enzyme conjugate, a washing solution, a substrate color developing solution anda stopping solution. An anti-EgAgB8/2 protein monoclonal antibody is prepared through the following method: firstly, amplifying EgAgB8/2 gene cDNA (complementary Deoxyribonucleic Acid) by utilizing RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and cloning an EgAgB8/2 gene; constructing a prokaryotic expression vector pET/EgAgB8/2 and expressing in escherichia coli BL21(DE3); inducing the expression of the prokaryotic expression vector by utilizing IPTG (isopropyl-beta-d-thiogalactoside); centrifuging and collecting thalli; after dissolving with a bacterium lysis solution and carrying out ultrasonic crushing; centrifuging and collecting supernatant; purifying by utilizing Ni-NTA glue; immunizing a BALB/c mouse by utilizing the purified EgAgB8/2 protein, so as to obtain a hybridoma cell line capable of stably secreting the anti-EgAgB8/2 protein monoclonal antibody; selecting the excellent monoclonal antibody to produce an antibody and purifying. The kit disclosed by the invention can be used for qualitative detection of an animal echinococcosis granulosa antigen and has the advantages of stable antibody source, good specificity, high sensitivity and good repeatability.

The invention discloses an echinococcosis granulosa ELISA antibody detection kit. The detection kit comprise an EgAgB8/2 recombinant antigen pre-coating enzyme label plate, a sample weak solution, positive contrast liquid, negative contrast liquid, an enzyme conjugate, washing liquid, a substrate coloring solution and a stopping solution. A method for preparing the EgAgB8/2 recombinant antigen comprises the following steps: using RT-PCR for amplifying EgAgB8/2 gene cDNA, cloning an EgAgB8/2 gene, constructing a prokaryotic expression vector pET-EgAgB8/2, and performing expression in escherichia coli BL21(DE3), using the IPTG-induced prokaryotic expression vector for expression, performing centrifugation to collect thalline, using a bacteria lysate for dissolving, and performing ultrasonicfragmentation, performing centrifugal collection of a supernatant, and using a Ni-NTA glue for purification. The kit takes recombinant protein EgAgB8/2 as antigen to detect an animal echinococcosis granulosa antibody, can be used for qualitative detection of the animal echinococcosis granulosa antibody, and has the advantages of stable antigen source, good specificity, high sensitivity, and good repeatability.

RNA, preferably messenger RNA, is purified by use of selective precipitation, preferably by addition of compaction agents. Also included is a scaleable method for the liquid-phase separation of DNA from RNA. RNA may also be recovered by fractional precipitation according to the invention. We have discovered that specific classes of compounds are unexpectedly potent in causing selective precipitation of DNA away from RNA, at low concentrations and in the presence of relatively elevated ionic strength. Modification of the selective removal of DNA can also remove both RNA and DNA, leaving behind a mixture which is advantageous for the further purification of, e.g., proteins. Additional aspects of the invention include mini-preps, preferably of RNA or of plasmid and chromosomal DNA to obtain sequenceable and restriction digestible DNA in high yields in multiple simultaneous procedures. Still further aspects disclose enhanced stripping of the compaction agent by a stripping method comprising high salt addition and pH shift, and combinations of these techniques. RNA Abstract Additionally, a new approach to the isolation of RNA from bacterial lysates employs selective precipitation by compaction agents. The above techniques can be enhanced by use of phase transfer catalysts (PTCs), most preferably selected polyamines of U.S. Pat. No. 6,617,108 polyamines which are quaternary compounds. (The use of PTCs in biotechnical processes is not evident in the literature, see e.g. www./ptorganics.com as of 27 Jan. 2005.)

The present invention concerns a method for the cosmetic caring of the skin and/or mucosa, comprising applying a postbiotic composition comprising at least one postbiotic and at least one bacteriocin and/or endolysin, wherein said postbiotic is preferably a bacterial lysate preferably obtained from bacteria heterologously expressing said at least one bacteriocin and/or endolysin and wherein said postbiotic and said at least one bacteriocin and/or endolysin have a synergistic effect in the cosmetic caring method.

The present invention is to obtain a novel gene that encodes a protein having a function of promoting acetic acid bacterial growth in the presence of a high concentration of acetic acid; to enhance acetic acid bacteria's function of promoting growth and thereby to develop acetic acid bacteria with an improved fermentation ability in the presence of a high concentration of acetic acid; and to provide a method for efficiently producing vinegar that contains a high concentration of acetic acid in a short time using the acetic acid bacterium. A novel gene having a function of improving the function of promoting growth at a practical level was cloned from acetic acid bacteria for practical use, belonging to the genus Gluconacetobacter, by a method comprising exposing acetic acid bacterial lysate to the presence of a high concentration of acetic acid; obtaining a gene of a protein that was not insolubilized but remained soluble; and thereby obtaining a gene having a function of promoting acetic acid bacterial growth in the presence of acetic acid. When cultured in the presence of acetic acid and ethanol, a transformant of acetic acid bacteria transformed with the gene was confirmed to have a significant effect of promoting growth.