The invention discloses a method for improving production of a lignocellulosic
enzyme line from filamentous fungi and an application of the filamentous fungi in
fermentation of microbial bio-based chemicals, and belongs to the field of
genetic engineering. According to the method, high-affinity
glucose transporter of the filamentous fungi is transformed by virtue of such
genetic engineering approaches as knocking-out,
mutation, importing and the like, and an obtained
engineering strain has the advantages. The filamentous fungi include, but not limited in,
neurospora, myceliophthora,
trichoderma,
aspergillus,
penicillin,
fusarium and the like; genes of the
transporter include NCU10021 (HGT-1) and a
homologous gene thereof as well as NCU04963 (HGT-2) and a
homologous gene thereof; any one or two genes are knocked out, or bases of any one or two genes are mutated, or expression of any one or two genes is decreased. The
engineering strain, which is obtained by importing the
transporter into a microbial strain in an exogenous mode or by changing endogenous expression of the
transporter, can
gain or enhance a glucose transporting and utilizing capacity, and subsequently, a capacity of producing such bio-based chemicals as
organic acid, organic
alcohol and the like through
fermentation can be gained or improved.