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Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger

A technology of Bacillus subtilis and fermentation liquid, which is applied in the direction of plant growth regulators, methods based on microorganisms, chemicals for biological control, etc., to achieve good inhibitory effect, short cycle, and reduce residue effects

Inactive Publication Date: 2013-10-23
DALIAN SANYI ANIMAL MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far there is no relevant report about the inhibition of Penicillium and Aspergillus niger by Bacillus subtilis fermentation broth

Method used

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  • Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger
  • Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger
  • Application of fermentation liquor of bacillus subtilis in inhibition of growth of penicillium and aspergillus niger

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Embodiment Construction

[0014] Preparation of Bacillus subtilis ( Bacillus subtilis ) strain (CICCNo.10321) fermentation broth:

[0015] a. Scraping Bacillus subtilis ( Bacillus subtilis ) bacterial strain (CICCNo.10321) was streaked on an agar plate, and after 24 hours of cultivation, a single colony was picked and inoculated in a liquid medium for 24 hours. The liquid medium contained: tryptone 18-20g, yeast powder per liter of water 8~10g, glucose 6~8, dipotassium hydrogen phosphate 4~6g, pH value 6.5~7.5;

[0016] b. Inoculate the bacterial liquid again in the same liquid medium as in step a, with an inoculum volume of 5-10%, at 37°C, 180-220r / min, and cultivate for 24-48 hours;

[0017] c. Centrifuge the obtained culture solution at 8000r / min for 5min, filter and sterilize with a 0.22μm membrane filter to obtain the supernatant, namely the fermentation broth.

[0018] Bacillus subtilis ( Bacillus subtilis ) strain (CICCNo.10321) fermentation broth to penicillium drug susceptibility test (pun...

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Abstract

The invention discloses application of fermentation liquor of bacillus subtilis (CICC No. 10321) in inhibition of growth of penicillium and aspergillus niger. The bacillus subtilis (CICC No. 10321) plays a role in inhibiting penicillium and aspergillus niger (the penicillium and aspergillus niger inhibiting circle is greater than 20 cm), the inhibition effect is good, and the duration is long. The bacillus subtilis is simple in culture, short in cycle and low in cost, is suitable for serving as a mold inhibitor and is used for inhibiting the growth of penicillium and aspergillus niger in easily-mildewed goods, such as feed and the like, and the residues of mycotoxins in animal foods are reduced effectively.

Description

technical field [0001] The invention relates to a new application of a Bacillus subtilis fermentation liquid, in particular to a use of the Bacillus subtilis fermentation liquid for inhibiting the growth of Penicillium and Aspergillus niger. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by molds during their growth, mainly including aflatoxins, trichothecenes (such as T-2 toxin, fusarium solanifolia and deoxynivalenol), gibberella zea enone, ochratoxin A and fumonisin, etc. According to the statistics of relevant departments, the pollution rates of vomitoxin, T-2 toxin and zearalenone in 194 feeds and raw materials such as corn and wheat bran are 50.0%, 18.1% and 26% respectively (mycotoxin organisms in feed Research progress of degradation [J]. Chinese Agricultural Sciences 2012, 45 (1): 153-158). Mycotoxins are mainly toxic to the liver and kidneys of animals. After the animals ingest mildewed feed, they can cause a series of body reac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/125
Inventor 庄国宏宋涛王效禹张琪
Owner DALIAN SANYI ANIMAL MEDICINE CO LTD
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