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122 results about "Vomitoxin" patented technology
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Vomitoxin, also known as deoxynivalenol (DON), is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale. The occurrence of deoxynivalenol is associated primarily with Fusarium graminearum (Gibberella zeae) and F. culmorum, both of which are important plant pathogens which cause fusarium head blight in wheat and gibberella or fusarium ear blight in corn. A direct relationship between the incidence of fusarium head blight and contamination of wheat with deoxynivalenol has been established. The incidence of fusarium head blight is strongly associated with moisture at the time of flowering (anthesis), and the timing of rainfall, rather than the amount, is the most critical factor. However, increased amount of moisture towards harvest time has been associated with lower amount of vomitoxin in wheat grain due to leaching of toxins. Furthermore, deoxynivalenol contents are significantly affected by the susceptibility of cultivars towards Fusarium species, previous crop, tillage practices, and fungicide use. It occurs abundantly in grains in Norway due to heavy rainfall.
Composite functional bacterial agent for preventing and controlling wheat scab and vomitoxin and application of composite functional bacterial agent
The invention discloses a composite functional bacterial agent for preventing and controlling wheat scab and vomitoxin and application of the composite functional bacterial agent. The composite functional bacterial agent is applied to wheat scab and deoxynivalenol pollution caused by fusarium graminearum. The composite functional bacterial agent comprises a biocontrol strain bacillus subtilis DC-1 (Bacillus subtilis DC-1) and a degradation strain nocardia sp. MOZ-1 (Nocardia sp. MOZ-1), and the composite functional bacterial agent is prepared from the biocontrol strain bacillus subtilis DC-1, the degradation strain nocardia sp. MOZ-1 and the degradation strain nocardia sp. MOZ-1. The complex microbial inoculant can significantly inhibit infection of fusarium graminearum and reduce accumulation of DON in wheat, is low in production cost, reduces use of chemical pesticides and pollution of biotoxins in agricultural products, and can be effectively applied to the field of agricultural biological control.
Owner:ZHEJIANG UNIV
Preparation method and application of bacterium-enzyme-magnetic nano-cluster composite detoxification agent
The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.
Owner:HENAN QIULE SEEDS TECH CO LTD
Bacterial strain for reducing vomitoxin function of wheat and corn and application of bacterial strain
The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.
Owner:SHANGHAI XINNONG YUNFU AGRI TECH CO LTD
Aspergillus niger recombinant strain for degrading vomitoxin and application thereof
The invention discloses an aspergillus niger recombinant strain for degrading vomitoxin and application thereof, and belongs to the field of gene engineering. In order to solve the problem that a method for efficiently degrading vomitoxin is lacked in the prior art, the invention provides an aspergillus niger recombinant strain for degrading vomitoxin, the aspergillus niger strain for co-expressing FsTRI12 is taken as a recipient bacterium, a vomitoxin acetyltransferase gene segment is selected, an intracellular gene expression vector is constructed, and a homozygous aspergillus niger recombinant strain is screened. The aspergillus niger recombinant strain provided by the invention can be applied to degradation of vomitoxin, provides a new choice for effectively solving the vomitoxin pollution problem in cereal raw materials and feeds, reducing grain loss and guaranteeing food and feed safety, and has potential industrial application prospects.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Enterococcus faecium capable of simultaneously degrading three mycotoxins and application of enterococcus faecium
The invention discloses enterococcus faecium capable of degrading three mycotoxins at the same time and application of the enterococcus faecium, and belongs to the technical field of microorganisms. The enterococcus faecium JBHBIO-BSC1 disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the enterococcus faecium JBHBIO-BSC1 is CCTCC (China Center for Type Culture Collection) NO: M 20241918. The enterococcus faecium disclosed by the invention has the most remarkable characteristic that the enterococcus faecium has an obvious degradation effect on the aflatoxin, the vomitoxin and the zearalenone, and the degradation rates of the aflatoxin, the vomitoxin and the zearalenone can respectively reach 74.67%, 85.83% and 82.12%. The enterococcus faecium disclosed by the invention is natural, safe, acid-resistant, alkali-resistant and high-temperature-resistant, and has relatively strong organic acid production capacity. In addition, the strain has an obvious inhibition effect on escherichia coli, salmonella and staphylococcus aureus, and is expected to be applied to the fields of food processing and microbial pharmacy.
Owner:HENAN JINBAIHE BIOTECH CO LTD
Bacteroides ovatus L2 for relieving vomitoxin toxicity and application of bacteroides ovatus L2
The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Apparatus for dynamic degradation of cereal vomitoxin based on ozone circulation
The utility model relates to grain safety processing equipment especially based on the equipment of dynamic degradation grain vomit toxin of ozone circulation, this equipment contains vertical treatment tower (1), ozone gas device contains the middle part cloth gas pipe (17) of middle part arrangement in vertical treatment tower (1), and the middle part cloth gas pipe (17) contains ozone entrance and cloth gas pipe with hole, grain circulation flow device contains the lower screw conveyer (5) for transportation, bucket elevator (8) and upper screw conveyer (18), bucket elevator (8) is arranged in the side of vertical treatment tower (1), and upper screw conveyer (18) is located above vertical treatment tower (1), and lower screw conveyer (5) is located below vertical treatment tower (1), and lower screw conveyer (5), bucket elevator (8) and upper screw conveyer (18) and vertical treatment tower (1) are combined as a whole material conveying circulation link.
Owner:ZHEJIANG SHANNONG MODERN AGRICULTURAL SCIENCE & TECHNOLOGY DEVELOPMENT CO LTD +2
Application of LW6 in resisting vomitoxin
The invention discloses application of LW6 in resisting vomitoxin, and belongs to the field of biological agents. Animal experiments are carried out on male C57BL mice, LW6 and DON are injected in a gavage mode to explore the DON tolerance influence of the mice, it is found that the LW6 can reduce harm to animal bodies caused by DON toxicity, and the DON tolerance of animals is improved. Therefore, the LW6 can be applied to a feed additive or developed into an anti-DON drug so as to reduce the toxic influence of DON on animals. The invention provides a potential solution way for preventing adverse reaction caused by DON, and provides a theoretical basis for preventing and controlling harm of DON to pig industry.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY +1
Bacillus subtilis, complex microbial inoculant and application of bacillus subtilis and complex microbial inoculant in degradation of mycotoxin
The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.
Owner:SHANDONG BAOLAI-LEELAI BIOENGINEERING CO LTD (CN)
Method for improving nutritional quality of wheat and reducing vomitoxin by combining cold plasma with lactobacillus fermentation
The invention discloses a method for improving the nutritional quality of wheat and reducing vomitoxin by combining cold plasma with lactic acid bacteria fermentation. The method comprises the following specific steps: (1) putting wheat grains polluted by DON into a reaction cavity of cold plasma equipment; (2) preparing a single or compound lactic acid bacteria starter; (3) mixing the wheat subjected to cold plasma treatment with a lactic acid bacteria leavening agent for fermentation; and (4) drying, grinding and sieving the fermented wheat to prepare the wheat flour meeting the standard. Through progressive cooperative treatment of cold plasma pretreatment and compound lactobacillus fermentation, the final degradation rate of DON reaches 91.04%, functional components (resistant starch reaches 13.71% and dietary fiber reaches 13.14%) are synchronously increased, and phytic acid (lowest to 0.42%) is greatly degraded to improve the bioavailability of mineral substances. Besides, the method can optimize the wheat processing performance, the prepared noodles are moderate in hardness, good in elasticity and stretch-proof, no chemical reagent is added in the whole process, collaborative optimization of efficient detoxification, nutrient enrichment and processing performance improvement is achieved, and a green and economical solution is provided for safe utilization of polluted wheat.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
DDS pulp capable of degrading vomitoxin and glycerol and preparation method of DDS pulp
The invention relates to the technical field of deep processing of agricultural and sideline products and feed additives, in particular to DDS pulp capable of degrading vomitoxin and glycerol and a preparation method of the DDS pulp. According to the method, the DDS pulp is inoculated with the specifically screened complex microbial inoculants, liquid fermentation is carried out under proper conditions, microorganisms preferentially utilize free glycerol in the DDS pulp as a carbon source for metabolism, the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the method, when the complex microbial inoculant is inoculated, the DDS pulp is inoculated with a functional strain with a vomitoxin degradation function and a special enzyme preparation, the functional strain is colonized in a system and continuously produces enzyme, and the added enzyme preparation can quickly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the degradation efficiency of the vomitoxin is improved. And the degradation product is converted into a non-toxic or low-toxicity degradation product.
Owner:HAILUN BAIZUN BIOENGINEERING CO LTD +1
Nitrate reduction oceanobacter sp. And application thereof
The invention relates to nitrate reduction pelagic bacteria and application thereof. The nitrate reductive ocean bacillus can be fermented to produce pyrroloquinoline quinone (PQQ) and trichothecenes toxin degrading enzyme, the trichothecenes toxin degrading enzyme mainly exists in bacterial cell lysis buffer, and the degradation rate of the trichothecenes toxin degrading enzyme to vomitoxin is 83%. The bacterial strain fermentation liquor can be used for degrading 39.3 percent of nivalenol and 100 percent of 15-acetyl deoxynivalenol. The strain or the trichothecene toxin degrading enzyme produced by the strain can be used for complete feed, concentrated feed, premixed feed, feed raw materials such as grain, corn, peanut meal, wheat and oat, silage, grain, grain and oil, grain processing and agricultural and sideline products, aged grain, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour and corn flour. Through biodegradation of industrial ethanol processing byproducts and trichothecenes in food, the quality safety of feed and food can be improved.
Owner:CHINA AGRI UNIV
Polypeptide derived from rapeseeds and capable of relieving injury of vomitoxin to piglet intestines and application of polypeptide
The invention discloses a rapeseed-derived polypeptide capable of relieving injury of vomitoxin to piglet intestines and application of the rapeseed-derived polypeptide. The rapeseed polypeptide provided by the invention is prepared from the following peptide fragments: YLKDPSKF, IVGI, KGFF, GHQYIVT, YYF, FHLY, STFSEY, SVDKQIAK, FYSI and ALRAPLGQN. The rapeseed polypeptide has the advantages that the rapeseed polypeptide is prepared from the following peptide fragments; the method comprises the following steps: extracting rapeseed protein isolate from fat rapeseed dregs, and carrying out enzymolysis, dialysis and ultrafiltration to obtain the micromolecular rapeseed polypeptide with the molecular weight of less than 1kDa. Researches show that the polypeptide can relieve piglet weight loss caused by vomitoxin intestinal injury, can reduce the concentration of proinflammatory factors in serum, and can relieve inflammatory factor infiltration; meanwhile, the rapeseed polypeptide can protect the jejunum tissue of the piglet and reverse jejunum tissue crypt hypertrophy and intestinal villus shortening caused by a DON model group, has an intestinal tissue injury recovery effect, and can be applied to deep processing of rapeseed meal and relieving vomitoxin-induced piglet intestinal injury.
Owner:GUANGDONG UNIV OF TECH
A labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection
The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.
Owner:GUANGZHOU INST FOR FOOD INSPECTION(GUANGZHOU INSPECTION CENT FOR WINE & SPIRITS)
Application of wheat TaALIX protein in reduction of vomitoxin accumulation and wheat gibberellic disease resistance
The invention relates to the field of biotechnology and plant protection, and discloses application of ESCRT related protein in prevention and treatment of wheat scab. It is found for the first time that by improving the expression quantity of the wheat ESCRT III protein TaALIX, the resistance of wheat to gibberellic disease is enhanced, the content of deoxynivalenol (DON) is reduced, and the application has practical significance in plant disease control.
Owner:NANJING AGRICULTURAL UNIVERSITY
Microbial composition for preventing and treating vomitoxin as well as preparation method and application of microbial composition
The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.
Owner:CHENGDU HUAHONG ECOLOGICAL AGRI TECH CO LTD
Application of modified montmorillonite in the adsorption of mycotoxins
The present invention discloses a modified montmorillonite for use in mycotoxin adsorption. The invention utilizes a specific organic solvent for thermal swelling and exfoliation treatment of the montmorillonite, exposing more adsorption active sites on the montmorillonite's two-dimensional surface. This increases the specific surface area of the montmorillonite nanosheets and enhances their adsorption capacity for weakly polar compounds. Combined with thermal activation and ultrasonic treatment, the modified montmorillonite further enhances its surface area, ultra-thin montmorillonite nanosheets, and a high proportion of coordinatively unsaturated surface sites. This increased number of active sites enables the modified montmorillonite to exhibit strong adsorption capacity in mycotoxin adsorption applications, particularly significantly improving adsorption efficiency for weakly polar mycotoxins such as zearalenone and vomitoxin by at least 93.17% and 49.31%, respectively, compared to unmodified sodium montmorillonite.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Efficient degradation method for mycotoxin in corn steep liquor
The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor, and belongs to the technical field of corn deep processing and detoxification. The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor. The method comprises the following steps: adjusting the pH value of thick corn steep liquor by using an alkaline solution; adding a solid catalyst into the corn steep liquor; carrying out detoxification treatment by combining heterogeneous ozone oxidation reaction with microbubbles; and separating and recovering the solid catalyst to obtain the detoxified corn steep liquor. The vomitoxin content of the treated concentrated corn pulp is smaller than 1000 g / kg, the degradation rate is larger than 93%, the zearalenone toxin content is smaller than 33 g / kg, and most of the zearalenone toxin is removed. According to the method disclosed by the invention, the content of fungaltoxin in the corn steep liquor is reduced, so that the economic value of corn byproducts is improved, the economic benefit of grain trade is favorably improved, and the storage period and value of produced products are improved.
Owner:SHANDONG FUYANG BIO-TECH CO LTD
Devosia sp. D-G15 and application thereof
The application isolates a new strain of Devosia genus bacteria from the soil of wheat field infected by Fusarium, and names it as Devosia sp. D-G15 (D-G15). The ANI value of D-G15 and other strains in the Devosia genus is less than 80%, which indicates that it is a new species of Devosia strain. The strain has been preserved in Guangdong Microbial Culture Collection Center on March 26, 2024, and the preservation number is GDMCC NO: 64453. The strain D-G15 can degrade vomitoxin (DON) into low-toxicity products, and the degradation of the strain to DON is beneficial within the range of pH value of 4-10 and temperature of 15-35 DEG C. The degradation activity of D-G15 is stable, the metabolic capacity is strong, and it is safe in the animal body, and can be used for the preparation of DON detoxification preparation and applied to the fields of feed processing and food processing.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Application of bacillus subtilis in relieving growth performance reduction and tissue and organ injury of yellow feather broiler chicken caused by vomitoxin
The invention discloses application of bacillus subtilis in relieving growth performance reduction and tissue and organ damage of yellow-feathered broilers caused by vomitoxin, and belongs to the field of feed additives. The bacillus subtilis is used for treating the yellow feather broilers eating DON polluted feed, and the bacillus subtilis is found to be capable of remarkably reducing the vomitoxin content in cecum mucosa, cecum content, serum, liver and excrement of the vomitoxin infected yellow feather broilers; the growth performance reduction, liver injury and intestinal injury caused by vomitoxin toxicity of the yellow feather broilers are relieved. The bacillus subtilis plays an important role in early discovery and treatment of yellow feather broilers infected by vomitoxin.
Owner:ZHEJIANG HUIJIA BIOTECH CO LTD +2
Composite mildew removing agent as well as preparation method and application thereof
The invention relates to a composite mildew removing agent as well as a preparation method and application thereof, relates to the technical field of mildew removing agents, and aims at solving at least one of the problems that an existing mildew removing agent is low in vomitoxin and zearalenone adsorption rate, low in degradation capacity, high in cost, unstable in performance and the like. The composite mildew removing agent is prepared from the following raw materials in percentage by mass: 30 to 35 percent of calcium-based montmorillonite, 32 to 42.5 percent of sodium-based montmorillonite, 10 to 15 percent of zinc-loaded montmorillonite, 10 to 15 percent of yeast cell wall, 2 to 5 percent of L2-ascorbic acid, 1 to 2 percent of chitosan oligosaccharide, 0.5 to 1 percent of lactobacillus plantarum and 0.0005 to 0.002 percent of mangiferin. According to the composite mildew removing agent, the calcium-based montmorillonite, the sodium-based montmorillonite and the zinc-loaded montmorillonite have a synergistic effect, so that a multi-stage adsorption network is formed, the space barrier effect is enhanced, two toxins DON and ZEN in feed can be effectively removed, the loss that the feed cannot be used or needs to be degraded for use due to toxin pollution is reduced, the utilization rate of the feed is increased, and the feed quality is improved. The production cost of feed enterprises is reduced.
Owner:BEIJING TAOGUTU NEW MATERIAL CO LTD
Application of kynurenic acid in product for preventing and treating vomitoxin toxicity
The invention discloses kynurenic acid with an effect of relieving vomitoxin toxicity, and belongs to the technical field of agricultural animals. The application proves that the kynurenic acid can effectively relieve the toxic effect induced by vomitoxin, and the kynurenic acid mainly has the effects of effectively promoting the weight gain of animals, improving the intestinal motility function, inhibiting the release of inflammatory factors, regulating intestinal flora and the like. The invention determines the effect of kynurenic acid in relieving vomitoxin toxicity, and the kynurenic acid has a wide application prospect in preparation of products for preventing and treating vomitoxin toxicity.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Composite vomitoxin detoxification agent as well as preparation method and application thereof
The invention relates to a compound vomitoxin detoxification agent as well as a preparation method and application thereof, and belongs to the field of feed additives. The compound vomitoxin detoxification agent comprises the following components: detoxification bacteria, glucose oxidase, an adsorbent and a eucommia ulmoides extract, the detoxification bacteria are lactobacillus plantarum, pediococcus pentosaceus and saccharomyces cerevisiae. The compound vomitoxin detoxification agent can effectively reduce DON toxins in feed, improve the antioxidant capacity of animals, improve immunity, regulate intestinal flora and promote animal growth.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Use of armillaria in degrading vomitoxin
The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.
Owner:JILIN AGRICULTURAL UNIV
A Bacillus cereus vomitoxin separation device and culture method
ActiveCN119432563BBioreactor/fermenter combinationsBiological substance pretreatmentsBiotechnologyCulture fluid
The present invention relates to the technical field of Bacillus cereus, and particularly relates to a separation device and a culture method for Bacillus cereus vomitoxin, including a culture hopper, with support columns arranged at the four corners of the bottom end of the culture hopper, and a cover plate arranged at the top of the culture hopper; a culture assembly, which is arranged inside the culture hopper and the cover plate; a separation assembly, which is arranged below the culture hopper; a gap opening and closing assembly, which is arranged between the culture hopper and the separation assembly, and the gap opening and closing assembly is used to send the preliminarily separated Bacillus cereus vomitoxin into the separation assembly; compared with the prior art, by setting a culture assembly, the present invention avoids the phenomenon of liquid culture medium stratification or precipitation. The preliminary screening module preliminarily separates the produced vomitoxin during the stirring process, effectively separates the smaller particle toxins from the bacteria, reduces the processing pressure of the subsequent separation assembly, and improves the separation efficiency.
Owner:TAIZHOU FOOD INSPECTION INST
Vomitoxin detoxification enzyme DH9-2 as well as coding gene and application thereof
The invention discloses a vomitoxin detoxification enzyme DH9-2 derived from Devosia sp. SD9-2 previously separated from yellow mealworm excrement, the amino acid sequence of the vomitoxin detoxification enzyme DH9-2 is shown as SEQ ID NO: 2 in a sequence table, and the nucleotide sequence of the coding gene of the vomitoxin detoxification enzyme DH9-2 is shown as SEQ ID NO: 1 in the sequence table. The invention also discloses a recombinant expression vector containing the coding gene, a recombinant host cell transformed with the vector, and a method for producing the detoxification enzyme by using the host cell. The detoxification enzyme can efficiently oxidize vomitoxin into a metabolite 3-keto-vomitoxin with low toxicity by depending on the existence of Ca < 2 + > ions and PQQ coenzyme under the conditions that the temperature is 35 DEG C and the pH value is 9.0, and the degradation rate of 50ug / mL DON in vitro can reach 100%. The invention particularly discloses application of the enzyme to degradation of vomitoxin in tenebrio molitor wheat bran feed and the like, and a new and effective biotechnology tool is provided for solving the problem of DON pollution in tenebrio molitor breeding and guaranteeing feed safety and tenebrio molitor health.
Owner:宁夏回族自治区粮油产品质量检测中心 +1
A method for constructing a yeast surface display of a two-enzyme system and its application in cascade detoxification of vomitoxin
The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Kit and detection method for detecting vomitoxin
The present invention belongs to the technical field of mycotoxin detection, and particularly relates to a kit and a detection method for detecting vomitoxin. The kit includes an antigen, a primary antibody, an enzyme-labeled secondary antibody, a chromogenic regulator, a phosphate buffer solution, and a detection reagent; the antigen is bovine serum albumin conjugated with vomitoxin; the primary antibody is a monoclonal antibody against vomitoxin; the enzyme-labeled secondary antibody is a goat anti-mouse IgG secondary antibody labeled with horseradish peroxidase; the chromogenic regulator is ethylenediaminetetraacetic acid or its sodium salt, or vitamin C; the detection reagent includes a TMB chromogenic solution. The present invention uses ethylenediaminetetraacetic acid or its sodium salt as a chromogenic regulator to change the chemical microenvironment around the chromogenic substance, enhance the optical properties of the chromogenic substance, and thereby reduce IC 50 , and improve the detection sensitivity.
Owner:QIQIHAR UNIVERSITY
A vomitoxin adsorbent, its preparation method and application
The present invention discloses a vomitoxin adsorbent, its preparation method and application, belonging to the field of feed additives. The vomitoxin adsorbent includes a carbonized intercalation composite material and an antibacterial component; the carbonized intercalation composite material uses montmorillonite powder as the main material, and chitosan is intercalated and compounded on the main material; the antibacterial component includes Bacillus subtilis, yeast cell wall, Isatis indigotica, Citrus reticulata Blanco, Lycium barbarum, Bupleurum chinense and Schisandra chinensis. The vomitoxin adsorbent prepared by the present invention integrates the characteristics of chitosan, sodium-based montmorillonite powder and Chinese herbal medicines in terms of antibacterial, anti-bacterial, astringent, mucosal repair, immune enhancement and adsorption of mycotoxins and forms a synergistic effect, which has good preventive and therapeutic effects on the contamination of vomitoxin in feed, can significantly reduce the negative effects brought by vomitoxin contamination, improve the growth efficiency, and can be widely applied in the field of feed additives. Moreover, the raw materials used are low-cost and easily available, have no residue, no drug resistance and no toxic and side effects, and have higher economic value in actual production.
Owner:SHENZHEN INST OF GUANGDONG OCEAN UNIV
Bacillus velezensis DSHBC4 and application thereof
The invention discloses bacillus velezensis DSHBC4 and application thereof, and belongs to the technical field of microorganisms. The invention aims to provide the bacillus velezensis capable of degrading vomitoxin, aflatoxin or zearalenone and inhibiting the growth of fusarium graminearum and aspergillus flavus. The invention provides bacillus velezensis DSHBC4, the bacillus velezensis is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M20242144, and the preservation time is October 8, 2024. A new solution is provided for biological control of corn crops, and the application potential is wider.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY