206 results about "Vomitoxin" patented technology

The invention provides Bacillus subtilis ANSB471 capable of effectively degrading vomitoxin in feed and an application of the Bacillus subtilis. The invention also relates to a cultural method of the Bacillus subtilis strain and a method for applying fermentation liquor of the strain to degradation on vomitoxin in the feed. After reacting for 72 hours, degradation rate of the bacillus subtilis ANSB471 on the vomitoxin in the feed is 95%, and the degradation rate on the vomitoxin is still about 90% after subculture. The strain also can secrete amylase and is high in enzyme activity, and utilization rate of animals on starch in the feed can be increased. The Bacillus subtilis ANSB471 has high vomitoxin degradation activity, strong specificity and mild effect and can not damage nutritional ingredients in the feed.

Methods are provided for isolating a nutrient from coffee cherries or for producing a food product that comprises a coffee cherry or portion thereof. It is particularly preferred that coffee cherries will have an extremely low concentration of mycotoxins, including various aflatoxins, fumonisins, ochratoxins, and / or vomitoxin (DON, deoxynivalenol).

Relating to the field of microorganisms, the invention discloses an inoculant, a feed or additive and a removal method for vomitoxin. The inoculant includes yeast and bacillus in a dry weight ratio of1:(0.5-15). According to the invention, the combined use of yeast and bacillus can efficiently remove vomitoxin, and the inoculant provided by the invention can be easily processed or added into vomitoxin contaminated cereals and / or feed, and then can effectively lower the content of vomitoxin in vomitoxin contaminated cereals and / or feed, can increase the utilization rate of cereals and / or feed,is beneficial to the health of raised animals, and can ensure the safety production of animal husbandry.

Methods are provided for isolating a nutrient from coffee cherries or for producing a food product that comprises a coffee cherry or portion thereof (FIG. 3). It is particularly preferred that coffee cherries will have an extremely low concentration of mycotoxins, including various aflatoxins, fumonisins, ochratoxins, and / or vomitoxin (DON, deoxynivalenol).

The present invention discloses a detoxification agent for vomitoxin in a biodegradable feed and a preparation technology thereof. The detoxification agent comprises the following raw materials: 40-60 parts of bentonite, 20 parts of immune polysaccharides, 10-20 parts of a free radical scavenger, and 20-30 parts of a vomitoxin degrading bacillus subtilis powder agent. A preparation method of the bacillus subtilis powder agent comprises the following steps: 1) original strains are subjected to a seed liquid activation and a mother culture is conducted in a primary fermentation tank loaded with a culture medium; 2) a mother culture liquid is subjected to an amplification culture in a second fermentation tank loaded with a LB culture medium; and 3) the bacterium body and the fermentation liquid are separated, the separated fermentation liquid is concentrated, and the concentrated fermentation liquid is dried to prepare the vomitoxin degrading bacillus subtilis powder agent; and the powder agent is combined with the two raw material mixtures in the above parts by weight to obtain the finished products. The detoxification agent has the degradation rate of the vomitoxin at 95% or more, is high in degradation efficiency, and avoids the defects of adsorbents. Besides, the immune polysaccharides and free radical scavenging components are added in the products, so that the detoxification agent can play the functions of protecting liver and improving the immunity of body, and is obvious in effects.

The invention discloses a various mycotoxin quantifying detection protein chip and a kit thereof. The chip comprises a substrate and a point coating layer of arrayed mycotoxin antibodies which contains seven mycotoxin antibodies formed by uniformly distributing anti-aflatoxin B1, anti-ochratoxin A, anti-fumonisins, anti-vomitoxin, anti-zearalenone, anti-T-2 toxin and anti-patulin on the substrate. A detecting result with various indications is acquired by reacting once by using the protein chip of the invention. The method can be widely applied to the safety sanitation detection for products such as popular food, grain and oil food, feeding stuff, juice beverage and the like and can meet the requirement of the modern food industry to rapidly, simply and efficiently detect the safety of food.

Relating to the field of removal of fungal toxins, the invention discloses a compound enzyme, an additive, application thereof and a method for removal of fungal toxins. Specifically, the invention relates to a compound enzyme, which contains amidase and esterase and can remove fungal toxins, especially fumonisins, ochratoxins and T2 toxin, additives containing the compound enzyme and applicationthereof in removal of fungal toxins, especially fumonisins, ochratoxins and T2 toxin, and a method for removal of fungal toxins. According to the technical scheme, combined use of the amidase and esterase can achieve simultaneous removal of ochratoxin A, fumonisins and T2 toxin, the removal efficiency is greatly improved than single use of one enzyme, and vomitoxins, aflatoxins and zearalenone toxin can be removed to certain degree.

A livestock feed supplement in which a core particle containing sodium metabisulfite and at least one binder is enrobed with an enteric coating, wherein the thickness and composition of the coating protects the sodium metabisulfite from decomposition to sulfur dioxide in an aqueous acid stomach environment. Also disclosed are a method of delivering sodium metabisulfite to the lower gastrointestinal tract of an animal, and a method of delivering an antidote to relieve the toxic effect of vomitoxin in an animal, by administering to the animal the livestock feed supplement.

The invention relates to a test paper card for synchronously detecting ochratoxin, vomitoxin and T-2 toxin, a preparation method and a detection method, and belongs to the field of immunological detection. The test paper card comprises a card shell and a test paper strip, wherein the test paper strip comprises a base plate, as well as an absorbent pad, a detection pad, a conjugate pad and a samplepad which are sequentially lapped and stuck to the base plate; the detection pad is an NC film provided with a quality control line, a detection line T1, a detection line T2 and a detection line T3;the quality control line coats a goat anti-mouse secondary antibody; the detection line T1 coats OTA-BSA; the detection line T2 coats DON-BSA; the detection line T3 coats T-2-BSA; the conjugate pad isa glass cellulose film, labeled by embedding time-resolved fluorescent microspheres, of an ochratoxin monoclonal antibody, a vomitoxin monoclonal antibody and a T-2 toxin monoclonal antibody; the sample pad is a dried glass cellulose film soaked by sample pad treating fluid. The invention provides a novel method for systematic, convenient and normalized quantitative synchronous detection of at least two fungaltoxins. The novel method has the advantages of good stability and high sensitivity.

The invention relates to a hybridoma cell strain AFM1B7, a monoclonal antibody thereof and an aflatoxin M1 flow lag immunization time-resolved fluorescence quick test kit. The hybridoma cell strain AFM1B7 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No.C201020. The monoclonal antibody secreted by the hybridoma cell strain AFM1B7 has high sensitivity and good specificity, the 50% inhibition concentration against aflatoxin M1 is 52pg / mL, and the cross reaction rate with aflatoxins B1, B2, G1 and G2, vomitoxin, zearalenone and fumonisin is less than 0.3%. The aflatoxin M1 flow lag immunization time-resolved fluorescence quick test kit prepared from the monoclonal antibody can be used for quantitatively measuring the content of aflatoxin M1, is simple and quick to operate, and has high accuracy.

The invention discloses a method for detecting vomitoxin and zearalenone in grains and feeds. The method comprises the following steps: A, adding 25g of a to-be-detected sample into a 250ml conical flask; B, adding 100ml of an extracting solution into the conical flask, and shaking the conical flask on a shaker for 20 minutes to obtain a mixed solution; C, adding 8ml of the mixed solution into a glass test tube, adding 80mul of acetic acid into the test tube, and performing column purification by virtue of a multifunctional purification column to obtain a purification solution; D, adding 250 microlitres of the purification solution into a container, adding deionized water containing 0.05 percent of acetic acid into the container for making up to volume to 1ml, thereby obtaining a test sample; and E, extracting the solution from the test sample, detecting by utilizing a multi-reaction detection mode of a liquid chromatography-tandem mass spectrometer, thereby obtaining data of the vomitoxin and the zearalenone respectively. According to the method, the operation steps have the time / labor-saving effect, the production and processing cost is reduced, and the detection result is relatively accurate and sensitive.

Relating to the field of microorganisms, the invention discloses a Bacillus licheniformis, an inoculant containing the same and application thereof, a vomitoxin degradation method and a kit. Specifically, the invention provides a Bacillus licheniformis strain with a preservation number of CGMCC NO.13314. The Bacillus licheniformis provided by the invention can efficiently and quickly degrade vomitoxin in grain and oil and / or feed, especially, when the vomitoxin content of grain and oil and / or feed is up to 50ppm or above, the Bacillus licheniformis also can efficiently and quickly degrade vomitoxin, and has no influence to the palatability of rain and oil and / or feed. At the same time, degradation of vomitoxin with the Bacillus licheniformis has no generation of toxic product, and is safeand environment-friendly. Therefore, the Bacillus licheniformis has good application prospects.

The invention relates to a production method for a livestock feed additive capable of adsorbing vomitoxin and protecting the intestinal tract. The production method comprises the following steps of 1, air-drying, mixing and coarsely crushing attapulgite clay, bentonite and zeolite ores, adding sodium propionate and sodium carbonate, and performing full mixing to obtain mixed small particles; 2, spraying water, and extruding the mixed small particles into a flaky clay mixture; 3, performing air-drying and beating, raising the temperature to 80 to 90 DEG C, performing high-speed shearing by using an emulsifier, and performing treatment to obtain emulsion by using a high-pressure homogenizer; 4, removing impurities by using a vibrating screen, performing grading by using a cyclone, and performing pressure filtration dehydration to obtain a cake; 5, performing air-drying, crushing and grinding to obtain the livestock feed additive capable of adsorbing vomitoxin and protecting the intestinal tract. According to the production method, the adopted ores are readily available, the production cost is low in cost, easy to control and suitable for industrial application, and the operating steps are simple; moreover, the obtained livestock feed additive has a good adsorption effect on vomitoxin, and the intestinal tract and the health of livestock can be protected.

A coffee cherry is harvested, preferably in a sub-ripe state, and quick-dried to provide a basis for numerous nutritional products. Such coffee cherries and portions thereof may be particularly characterized by their extremely low concentration of mycotoxins, including various aflatoxins, fumonisins, ochratoxins, and / or vomitoxin (DON, deoxynivalenol).

The invention discloses vomitoxin hybridoma, a monoclonal antibody, and a preparation method and application of the monoclonal antibody. The preparation method comprises the steps of: immunizing a Balb / c mouse with a vomitoxin and bovine serum albumin conjugate as an immunizing antigen; preparing a hybridoma by the spleen cells and the myeloma cell SP2 / 0 of the mouse; obtaining a monoclonal antibody hybridoma strain D-2-1CGMCC No. 5161 through an indirect ELISA (Enzyme-Linked Immunosorbent Assay) screening and a limiting dilution method; and injecting the hybridoma strain into the abdominal cavity of the mouse to produce a monoclonal antibody, wherein the monoclonal antibody is used for indirect competitive ELISA testing of the vomitoxin. The monoclonal antibody is capable of specifically detecting the vomitoxin, and high in sensitivity; and when an ELISA kit prepared by the monoclonal antibody is used for detecting the vomitoxin, the cost is low, large equipment is not needed, the operation is simple and convenient, and the preparation method is convenient for popularization and utilization.

The invention relates to a veterinary medicine composition for preventing and treating mycotoxin pathopoiesia. The veterinary medicine composition is prepared from the follow ingredients in parts by weight: 5 to 25 parts of mildew preventive, 5 to 25 parts of sodium humate, 5 to 25 parts of montmorillonite, 5 to 25 parts of rheum officinale, 5 to 25 parts of scutellaria baicalensis, 5 to 25 parts of coptis chinensis, 5 to 15 parts of liquorice and 5 to 25 parts of radix isatidis. The veterinary medicine composition has the advantages that the specific adsorption capacity is realized, various mycotoxins can be directionally and effectively eliminated, the veterinary medicine composition effectively aims at zearalenone, vomitoxin, aflatoxin, ochratoxin, fumitremorgin, T-2 toxin and the like, the detoxification capability of the liver per se on the toxin can be improved, and the toxin injury to animal bodies is eliminated.

The invention provides a method for reducing vomitoxin of wheat bran through extrusion. The method mainly comprises the following steps: 1, putting the wheat bran in water, adding alkali to adjust the wheat bran to be alkaline; 2, drying the alkaline wheat bran, then compounding the wheat bran and starch according to a certain proportion, and mixing evenly; 3, extruding and detoxifying a mixture of the wheat bran and the starch in an extruder; 4, neutralizing the alkali. The wheat bran powder prepared according to the method is high in content of dietary fibers and low in vomitoxin, can serve as an additive to be mixed with various other food base materials to prepare various foods with rich dietary fibers, and has a quite extensive scope of application.

The invention discloses a pantoea agglomerans strain and application thereof. The pantoea agglomerans strain is named as Pantoea agglomerans ZJU23, with a preservation number of CGMCC No.16174 and a preservation date of July 30, 2018. According to the pantoea agglomerans strain, the control effect on wheat scab is up to 50-70%, vomitoxin is reduced by 50-80%, diseases caused by plant pathogenic fungi such as gibberellic disease, gray mold, rice blast, colletotrichum destructivum and rice sheath blight disease can also be simultaneously controlled, and the range of controlling is wide.

The invention relates to a feed additive for prevention and treatment of livestock and poultry production performance decline, product quality decline, digestive system damage and easy infection of diseases caused by vomitoxin poisoning and application thereof. The active ingredients include, by weight, 10-20 parts of a DL-methionine hydroxy analogue, 6-10 parts of glutathione, 10-15 parts of immune polysaccharide, and 60-70 parts of bacillus mixed powder for degrading vomitoxin. The active ingredients are dried, crushed and mixed to obtain the feed additive product. The product is soluble inwater, and has no toxic or side effect, no drug resistance and no pollution. Feeding experiments verify that the feed additive product provided by the invention can effectively prevent and treat livestock and poultry production performance decline caused by vomitoxin, and also can lower the livestock and poultry mortality rate, improve the intestinal tract barrier function of livestock and poultry, and improve the livestock and poultry immunity.

The invention relates to a formula feed for mixedly breeding shrimps and a preparation method thereof. In the formula, fermented soybean meal is used, and anti-nutritional factors contained in soybean meal vegetable protein are effectively decreased; the advantage of improving palatability is achieved, and nutrient digestion and absorption and intestinal health are improved; by adding wall-broken yeast powder, the shrimp immunity can be improved, and bacteriotoxin, aflatoxin, vomitoxin and the like in the feed can be adsorbed; by adding the raw materials such as fish meal, domestic fish meal, shrimp meal, the fermented soybean meal and squid liver paste, nutrition arrangement is reasonable, the attractant effect is good, the absorption and utilization rate of the feed is effectively increased, and the feed safety is effectively improved; by adding tributyrin, epithelial cells of the shrimp intestines can be repaired, the intestinal health is maintained, and occurrence of enteritis is reduced; by adding bile acid and an agent beneficial for the liver and the gall, the shrimp liver health is effectively guaranteed, and the shrimp constitution is improved.

The invention discloses an immunoaffinity column for detecting vomitoxin and application of the immunoaffinity column. The immunoaffinity column comprises a solid-phase extraction column hollow column (1), an upper sieve plate (2), an upper cover (3), a lower cover (4), a solid-phase medium (5) and a lower sieve plate (6), wherein the solid-phase medium is coupled with a vomitoxin antibody. The invention further provides a method for detecting vomitoxin residues in grains and feed by adopting the immunoaffinity column for detecting vomitoxin. The immunoaffinity column disclosed by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening a lot of samples and monitoring in the field.

The invention belongs to the technical field of a biological antidote, and particularly relates to a biological antidote for relieving or treating harm of mycotoxin to cells. The biological antidote consists of 1*105 to 1*107 CFU / mL of bacillus licheniformis, 1*105 to 1*107 CFU / mL of bacillus coagulans and 1*105 to 1*107 CFU / mL of clostridium butyricum; the bacillus licheniformis, the bacillus coagulans and the clostridium butyricum are purchased from Guangdong Microbial Culture Collection Center; the preservation number of the bacillus licheniformis is GDMCC1.11, the preservation number of the bacillus coagulans is GDMCC1.645, and the preservation number of the clostridium butyricum is GDMCC1.676; and the mycotoxin is aflatoxin B1, zearalenone and vomitoxin. Based on a total inventive concept, the invention further discloses application of chlorogenic acid and composite probiotics which are used as the biological antidote for relieving or treating harm of the mycotoxin. the Accordingto the invention, the chlorogenic acid, composite probiotics or a composition of chlorogenic acid and composite probiotics is used for relieving the damage of mycotoxin to cells, so that the combineddetoxification of aflatoxin B1, the zearalenone and vomitoxin is implemented.

The invention relates to a mycotoxin biodegradation agent containing glucose oxidase and saccharomyces cerevisia and an application of the mycotoxin biodegradation agent. The problems of production ofa mycotoxin biodegradation agent, degradation of aflatoxin B1, zearalenone, vomitoxin and T-2 toxin, and realization of the application of the mycotoxin biodegradation agent to mildew and rot feeds for piggies and broilers can be effectively solved. The mycotoxin biodegradation agent is prepared from glucose oxidase and saccharomyces cerevisia yk18 fermentation cultures in a mass ratio of the glucose oxidase to the saccharomyces cerevisia yk18 fermentation cultures being 1 to (1-9), wherein the glucose oxidase is a powdery feed additive namely glucose oxidase, the enzyme activity is greater than or equal to 2000 u / g and the enzyme activity is greater than or equal to 5000 u / g, or the glucose oxidase is a liquid feed additive namely glucose oxidase, and the enzyme activity is greater thanor equal to 10000 u / mL; and the saccharomyces cerevisia yk18 fermentation cultures are prepared through the steps of activating saccharomyces cerevisia yk18 on a flat seed culture medium, then inoculating 2% by v / v of the activated saccharomyces cerevisia yk18 in liquid fermentation mediums, performing culturing, then adding 5% by v / v of the liquid fermentation mediums to obtain a saccharomyces cerevisia yk18 liquid fermentation culture solution, then performing sealed culture with solid culture mediums, and performing air drying. The method is simple, the mycotoxin biodegradation agent is easy to produce and prepare, and the degradation agent is high in enzyme activity and is a creation in the mycotoxin biodegradation agent.

The invention discloses a suspension chip detection method for simultaneously detecting various mycotoxins (aflatoxin B1, vomitoxin, T-2 toxin, and zearalenone). Complete antigens of the mycotoxins and carboxylated microspheres with different codes are coupled through an EDC method, and covalent binding is allowed. With a competition method, a suspension chip technology research is carried out. During detection, the conjugate and monoclonal antibodies of the mycotoxins are oscillated; standard products are added, such that corresponding small molecules of a detection target and the complete antigens compete for the limited monoclonal antibodies; centrifugation is carried out, and supernatant is removed; a biotinylated secondary antibody is added; centrifugation is carried out, and supernatant is removed; SA-PE is added, such that a composition of detection target antigen-monoclonal antibody-secondary antibody-SA-PE is obtained; a median fluorescence value is reduced with the increasing of the concentration of the small molecules, such that a multichannel competition standard curve is drawn. Methanol / water with a certain ratio is used for pre-treating peanut and corn, and multichannel standard curves in corn and peanut are respectively drawn with a negative treatment liquid as a diluting liquid. Through the researches on matrix effect, method accuracy, and method repeatability, various mycotoxins in corn and peanut can be simultaneously detected with the suspension chip method. With the process, a sensitivity reaches a pg-ng level, the result is stable and reliable, sample dose is low, and the method is simple and fast. According to the invention, a novel method is provided for the detection of various mycotoxins.

The invention discloses a method for simultaneously detecting multiple fungal toxins in wheat, and concretely relates to a method for detecting vomitoxin (DON), aflatoxin B1 (AFB1) and zearalenone (ZEN) in wheat through immunoaffinity purification-high performance liquid chromatography. The method comprises the following steps: extracting a sample by using an acetonitrile-water solution having a certain ratio, purifying the obtained sample solution by a composite immunoaffinity column, simultaneously detecting the three fungal toxins through adopting the high performance liquid chromatography,preparing a standard working solution to qualitatively and quantitatively detect the three fungal toxins. Compared with the prior art, the method adopting the immunoaffinity chromatography to greatlyimprove the purifying degree of the sample and adopting the high performance liquid chromatography to realize simultaneous detection of multiple toxins has the advantages of operating time saving, high sensitivity, accuracy in quantification and good repeatability.

The invention discloses a microorganism having a detoxifizyme activity and with fermentation residues applicable to feed. When a recombinant microorganism disclosed by the invention is fermented by taking mildewed grains or grains containing mycotoxins as fermentation raw materials, a starch utilization rate is at least 75%, and the recombinant microorganism contains exogenous mycotoxin detoxifizyme gene expression cassettes, wherein mycotoxin detoxifizyme is selected from the following detoxifizymes: aflatoxin detoxifizyme, zearalenone detoxifizyme, vomitoxin detoxifizyme, T-2 toxin detoxifizyme or a combination thereof; and in addition, compared with a wild strain or an original strain which is free from the exogenous mycotoxin detoxifizyme gene expression cassettes, the recombinant microorganism can increase a detoxifying activity for at least one mycotoxin by an increased range [delta] T which is more than or equal to 50%. According to the microorganism disclosed by the invention, a substrate using range is widened and cost of raw materials is reduced; an existing fermentation technology is adopted in the whole course of a degrading process and the addition of additional cost is avoided; and in addition, the value of a fermentation product can be also increased.

The invention discloses a Talaromyces purpureogenus strain, a biocontrol agent and application of the Talaromyces purpureogenus strain. The classification name of the Talaromyces purpureogenus strain is Talaromyces purpureogenus TP18, and the accession number of the Talaromyces purpureogenus strain is CGMCC No. 22403. The strain has a good antagonistic effect on fusarium graminearum, and can be applied to prevention and treatment of wheat scab; and the biocontrol agent prepared from the fermentation broth of the strain has a strong inhibition effect on spore germination, hypha growth and vomitoxin yield of Fusarium graminearum, and has good application prospects.

The invention discloses a kit for detecting vomitoxin in food. The kit is prepared from an enzyme-labelled antigen, a thinning liquid of the enzyme-labelled antigen, a vomitoxin monoclonal antibody, a vomitoxin series standard product solution, a chemical fluorescent primer solution A, a chemical fluorescent primer solution B, a concentration compound solution, and a concentration detergent. The kit has the advantages that the sensitivity and specificity are higher, and the detection sensitivity on the vomitoxin is 0.25mg / L.