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68 results about "Vomitoxin" patented technology

Vomitoxin, also known as deoxynivalenol (DON), is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale. The occurrence of deoxynivalenol is associated primarily with Fusarium graminearum (Gibberella zeae) and F. culmorum, both of which are important plant pathogens which cause fusarium head blight in wheat and gibberella or fusarium ear blight in corn. A direct relationship between the incidence of fusarium head blight and contamination of wheat with deoxynivalenol has been established. The incidence of fusarium head blight is strongly associated with moisture at the time of flowering (anthesis), and the timing of rainfall, rather than the amount, is the most critical factor. However, increased amount of moisture towards harvest time has been associated with lower amount of vomitoxin in wheat grain due to leaching of toxins. Furthermore, deoxynivalenol contents are significantly affected by the susceptibility of cultivars towards Fusarium species, previous crop, tillage practices, and fungicide use. It occurs abundantly in grains in Norway due to heavy rainfall.

Preparation method and application of bacterium-enzyme-magnetic nano-cluster composite detoxification agent

PendingCN121406630AHydrolasesWater contaminantsBacillus amyloliquefaciensAflatoxin degradation
The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.
Owner:HENAN QIULE SEEDS TECH CO LTD

Bacteroides ovatus L2 for relieving vomitoxin toxicity and application of bacteroides ovatus L2

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Apparatus for dynamic degradation of cereal vomitoxin based on ozone circulation

The utility model relates to grain safety processing equipment especially based on the equipment of dynamic degradation grain vomit toxin of ozone circulation, this equipment contains vertical treatment tower (1), ozone gas device contains the middle part cloth gas pipe (17) of middle part arrangement in vertical treatment tower (1), and the middle part cloth gas pipe (17) contains ozone entrance and cloth gas pipe with hole, grain circulation flow device contains the lower screw conveyer (5) for transportation, bucket elevator (8) and upper screw conveyer (18), bucket elevator (8) is arranged in the side of vertical treatment tower (1), and upper screw conveyer (18) is located above vertical treatment tower (1), and lower screw conveyer (5) is located below vertical treatment tower (1), and lower screw conveyer (5), bucket elevator (8) and upper screw conveyer (18) and vertical treatment tower (1) are combined as a whole material conveying circulation link.
Owner:ZHEJIANG SHANNONG MODERN AGRICULTURAL SCIENCE & TECHNOLOGY DEVELOPMENT CO LTD +2

Bacillus subtilis, complex microbial inoculant and application of bacillus subtilis and complex microbial inoculant in degradation of mycotoxin

PendingCN121379856AFungiBacteriaAspergillus ochraceopetaliformisAntagonism
The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.
Owner:SHANDONG BAOLAI-LEELAI BIOENGINEERING CO LTD (CN)

DDS pulp capable of degrading vomitoxin and glycerol and preparation method of DDS pulp

The invention relates to the technical field of deep processing of agricultural and sideline products and feed additives, in particular to DDS pulp capable of degrading vomitoxin and glycerol and a preparation method of the DDS pulp. According to the method, the DDS pulp is inoculated with the specifically screened complex microbial inoculants, liquid fermentation is carried out under proper conditions, microorganisms preferentially utilize free glycerol in the DDS pulp as a carbon source for metabolism, the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the method, when the complex microbial inoculant is inoculated, the DDS pulp is inoculated with a functional strain with a vomitoxin degradation function and a special enzyme preparation, the functional strain is colonized in a system and continuously produces enzyme, and the added enzyme preparation can quickly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the degradation efficiency of the vomitoxin is improved. And the degradation product is converted into a non-toxic or low-toxicity degradation product.
Owner:HAILUN BAIZUN BIOENGINEERING CO LTD +1

A labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection

ActiveCN115541870BMaterial analysisCarboxyl radicalBiological toxin
The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.
Owner:GUANGZHOU INST FOR FOOD INSPECTION(GUANGZHOU INSPECTION CENT FOR WINE & SPIRITS)

Microbial composition for preventing and treating vomitoxin as well as preparation method and application of microbial composition

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.
Owner:CHENGDU HUAHONG ECOLOGICAL AGRI TECH CO LTD

Efficient degradation method for mycotoxin in corn steep liquor

The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor, and belongs to the technical field of corn deep processing and detoxification. The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor. The method comprises the following steps: adjusting the pH value of thick corn steep liquor by using an alkaline solution; adding a solid catalyst into the corn steep liquor; carrying out detoxification treatment by combining heterogeneous ozone oxidation reaction with microbubbles; and separating and recovering the solid catalyst to obtain the detoxified corn steep liquor. The vomitoxin content of the treated concentrated corn pulp is smaller than 1000 g / kg, the degradation rate is larger than 93%, the zearalenone toxin content is smaller than 33 g / kg, and most of the zearalenone toxin is removed. According to the method disclosed by the invention, the content of fungaltoxin in the corn steep liquor is reduced, so that the economic value of corn byproducts is improved, the economic benefit of grain trade is favorably improved, and the storage period and value of produced products are improved.
Owner:SHANDONG FUYANG BIO-TECH CO LTD

Devosia sp. D-G15 and application thereof

The application isolates a new strain of Devosia genus bacteria from the soil of wheat field infected by Fusarium, and names it as Devosia sp. D-G15 (D-G15). The ANI value of D-G15 and other strains in the Devosia genus is less than 80%, which indicates that it is a new species of Devosia strain. The strain has been preserved in Guangdong Microbial Culture Collection Center on March 26, 2024, and the preservation number is GDMCC NO: 64453. The strain D-G15 can degrade vomitoxin (DON) into low-toxicity products, and the degradation of the strain to DON is beneficial within the range of pH value of 4-10 and temperature of 15-35 DEG C. The degradation activity of D-G15 is stable, the metabolic capacity is strong, and it is safe in the animal body, and can be used for the preparation of DON detoxification preparation and applied to the fields of feed processing and food processing.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Use of armillaria in degrading vomitoxin

The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.
Owner:JILIN AGRICULTURAL UNIV

A method for constructing a yeast surface display of a two-enzyme system and its application in cascade detoxification of vomitoxin

The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION

Quality safety evaluation method for non-grain type protein source feed

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.
Owner:NINGXIA UNIVERSITY +1

Application of Fuzhuan tea extract in prevention of piglet health injury caused by vomitoxin

The invention belongs to the technical field of animal nutrition and feed, discloses an application of a Fuzhuan tea extract in prevention of vomitoxin-caused piglet health injury, and particularly relates to an application of the Fuzhuan tea extract as a feed additive in prevention or improvement of piglet health injury. The feed additive can effectively reduce negative effects on weaned piglets caused by excessive vomitoxin in the feed, wherein the negative effects comprise growth performance reduction, diarrhea rate increase, intestinal morphology damage, intestinal cell proliferation inhibition, intestinal epithelial barrier injury, immune disorder, abnormal liver function and excessive oxidative stress and inflammatory response.
Owner:HUNAN AGRI UNIV +1

Silage additive for degrading mycotoxin and application thereof

The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.
Owner:CHINA AGRI UNIV

A vomitoxin detoxification enzyme, its encoding gene, and its applications

ActiveCN116064448BGibellina cerealisGene
This invention discloses a vomitoxin detoxification enzyme, its encoding gene, and its applications, belonging to the field of molecular biology. This invention discovered a wheat SOT gene, TaSOT2b. The TaSOT2b protein was obtained through in vitro induced expression, and its amino acid sequence is shown in SEQ ID NO.1. This invention found that this protein possesses in vitro enzymatic activity, capable of modifying DON into SON-3-sulfate, and can act as a vomitoxin detoxification enzyme. It has potential application value and reference significance for the scientific prevention and control of wheat scab and DON toxin contamination, and has broad application prospects in agriculture, feed, and industry.
Owner:山东国仓健生物科技有限公司

Application of glucuronolactone in the preparation of feed to alleviate spleen damage caused by combined PRRSV and vomitoxin

This invention belongs to the field of animal feed technology and discloses the application of glucuronolactone in the preparation of feed that alleviates spleen damage caused by the combined effects of PRRSV and DON. This invention discovers that glucuronolactone can effectively alleviate spleen damage in piglets caused by co-exposure to PRRSV and DON. It exerts its protective effect by activating the Nrf2 antioxidant signaling pathway, improving spleen tissue structure, alleviating oxidative stress, inhibiting inflammatory responses, and inhibiting apoptosis. This invention also provides a feed containing the aforementioned effective amount of glucuronolactone, with the basic feed tailored to the nutritional needs of piglets. This invention fills the technological gap in effective intervention methods for spleen damage under PRRSV and DON co-exposure. The glucuronolactone addition process is simple, the effect is significant, and it is suitable for large-scale feed production. It can effectively solve the problem of immunosuppression in pig herds caused by the combined harm of PRRSV and DON in the pig industry, and has important practical value.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Treatment system for reducing toxin content in corn water-soluble protein

The utility model relates to the technical field of corn soaking water treatment, in particular to a treatment system for reducing toxin content in corn water-soluble protein, which comprises a first filtering device communicated with a corn soaking water conveying pipeline, and a second filtering device communicated with a filtrate outlet of the first filtering device. A liquid outlet of the second filtering device is communicated with toxin adsorption equipment, a liquid outlet of the toxin adsorption equipment is communicated with a concentration device, a concentrated solution outlet of the concentration device is communicated with a third filtering device, a liquid outlet of the third filtering device is communicated with a drying device, and a discharge port of the drying device is communicated with a product storage tank. The treatment system is reasonable in design, the content of vomitoxin and fumonisins in the protein product can be effectively reduced through the toxin adsorption equipment, and the safety of the protein product is guaranteed.
Owner:ZHUCHENG HAOTIAN PHARMA CO LTD

Construction method and application of SERS (Surface Enhanced Raman Scattering) biosensor for detecting vomitoxin

The invention provides a construction method and application of an SERS (Surface Enhanced Raman Scattering) biosensor for detecting vomitoxin. The SERS biosensor is used for rapidly detecting the vomitoxin (DON) in real time. Ag is sputtered on the surface of the filter paper through a magnetron sputtering method, so that the surface of the filter paper has a Raman signal enhancement effect. An aptamer (Apt) of vomitoxin is selected as a recognition sensing element to specifically capture DON, Apt is connected to filter paper through an Ag-S bond, Au-4-ATP-cDNA is further modified and connected to the filter paper through complementation of Apt and cDNA, and 4-ATP serves as a signal molecule to provide a Raman signal. When the DON exists, the Apt is combined with the DON and is separated from the cDNA, the Raman signal of the 4-ATP is weakened after the Au-4-ATP-cDNA falls off, and the DON is quantitatively analyzed by detecting the Raman signal change of the 4-ATP. The sensor has high sensitivity, specificity and stability, provides a new idea for on-site rapid detection and real-time monitoring, and has application value and application prospect.
Owner:NANJING NORMAL UNIVERSITY

Composition for relieving vomitoxin harm of weaned piglets as well as preparation method and application of composition

The invention relates to the technical field of breeding, in particular to a composition for relieving vomitoxin harm of weaned piglets and a preparation method and application of the composition. The composition is prepared from the following components in parts by weight: 50 to 70 parts of glucurolactone, 10 to 15 parts of cynarin, 20 to 40 parts of scutellaria baicalensis flavone, 10 to 20 parts of stevia rebaudiana extract and 80 to 120 parts of a carrier. The composition can be used for effectively relieving the symptoms of diarrhea, weight loss and intestinal dysfunction of weaned piglets.
Owner:ZHUCHENG HAOTIAN PHARMA CO LTD

Vomitoxin hydrolase and method for degrading vomitoxin

The invention provides a vomitoxin hydrolase and a method for degrading vomitoxin. The vomitoxin hydrolase has an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence having more than 70% of homology with the SEQ ID NO: 1. The problem that in the prior art, a hydrolase preparation for degrading vomitoxin is poor in DON degradation effect can be solved, and the hydrolase preparation is suitable for the field of vomitoxin degradation.
Owner:ANGEL YEAST CO LTD +1

Efficient deoxynivalenol degrading enzyme mutant and application thereof

The invention discloses a sorbose dehydrogenase mutant capable of efficiently degrading vomitoxin, and belongs to the technical field of agricultural biology. The mutant is based on wild type SDH, through rational design and a directed evolution strategy, the mutant comprises three mutation sites F103A, T492E and N427A, the amino acid sequence and the nucleotide sequence of the mutant are SEQ ID NO.1 and SEQ ID NO.2 respectively, the mutant SDHF103A / T492E / N427A can efficiently degrade DON without depending on phenazine methyl sulfate (PMS), and the degradation activity of the mutant SDHF103A / T492E is improved by about two times compared with that of a double mutant SDHF103A / T492E under the condition that PMS does not exist. The method can be applied to preparation of the vomitoxin detoxification enzyme, and has a good application prospect in the aspect of biological detoxification of DON in feeds, grains and food.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Dvorstia and method for fermenting corn steep liquor using the same

The present application relates to the technical field of microorganism, and particularly relates to a Dvorst's bacteria and a method for fermenting corn steep liquor by using the same.The present application provides a Dvorst's bacteria capable of efficiently degrading vomitoxin in corn steep liquor, and corn steep liquor is fermented by using the Dvorst's bacteria and Candida krusei in cooperation, so that the pH of the corn steep liquor can be increased, the content of vomitoxin and anti-nutritional factors in the corn steep liquor can be reduced, and the content of choline in the corn steep liquor can be significantly increased, the quality of the corn steep liquor is obviously improved, the corn steep liquor after the cooperative fermentation can be better applied in the field of animal feed, and has a wide application prospect.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Detection device for mycotoxin in buffalo milk

The utility model discloses a buffalo milk fungaltoxin detection device, which relates to the technical field of buffalo milk detection, and comprises a detection table, a detection support plate and an electron microscope metal spraying instrument, the detection support plate is arranged on the outer side of the detection table, the detection support plate is used for accommodating parts for detection, and the electron microscope metal spraying instrument is arranged on the detection table. The detection supporting plate is arranged on the detection table, the detection supporting plate is moved on the detection table according to use requirements, a detection instrument can be conveniently stored and taken, a configuration mechanism is arranged over the detection table, the configuration mechanism is used for rotating and changing positions of a plurality of groups of glass beakers, and then a configured detection solution can be conveniently subjected to split charging treatment. Aiming at the current situation that the sensitivity of a product for screening vomitoxin in buffalo milk on site on the market is not high, an SERS technology with high sensitivity as a main characteristic is introduced into LFIA, and an SERS immunochromatographic analysis method based on a colorimetric and Raman dual-signal mode is developed and used for rapidly detecting DON in buffalo milk.
Owner:GUANGXI UNIV +1

Liquid chromatography / mass spectrometry detection method of 3-keto-vomitoxin

The invention provides a liquid chromatography-mass spectrometry method capable of efficiently detecting a deoxynivalenol (DON) metabolic intermediate product, namely 3-keto-DON (3-keto-DON). The detection method comprises a liquid phase elution procedure and a mass spectrum acquisition mode. According to the method, the detection time is 10 min, the retention time of 3-keto-DON is 6.99 min, the detection limit is 4 ng / ml, the quantitation limit is 10 ng / ml, the linear range is 10-1800 ng / ml, the correlation coefficient R2 is equal to 0.9995, and the relative standard deviation RSD is 1.99%.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

A compound preparation of traditional Chinese medicine and additive for treating adenitis of laying hens and application thereof

The application relates to a traditional Chinese medicine and additive compound preparation for treating gizzarditis of laying hens and application, and relates to the technical field of veterinary drugs. The compound preparation is prepared by mixing a traditional Chinese medicine extract and a functional feed additive. The raw material composition of the traditional Chinese medicine extract comprises torrefied malt, torrefied medicated leaven, radix codonopsis, rhizoma atractylodis macrocephalae, rhizoma coptidis, andrographis, dried tangerine or orange peel, white arillus, and liquorice. The additive comprises neutral protease, a mould inhibitor and probiotics. The mould inhibitor is selected from aspergillus flavus B1 degrading enzyme, corn strobukenone degrading enzyme, fumonisin degrading enzyme and vomitoxin degrading enzyme. The probiotics are selected from lactobacillus plantarum, bacillus subtilis and fructo-oligosaccharide. The application is based on the traditional Chinese veterinary theory of food digestion and spleen health, dampness expelling and qi regulation, heat clearing and toxin resolving and mucosa repairing. In combination with the effects of the additive on digestion, mould expelling and intestinal micro-ecological regulation, the application has a remarkable treatment effect on gizzarditis of laying hens, can improve the feed intake and weight gain, reduce the gizzard index, promote the repair of gastric mucosa, and has no antibiotic residue and no toxic side effect.
Owner:XINXIANG HEXIE ANIMAL PHARM CO LTD

Vomitoxin hydrolyzing enzyme and method of degrading vomitoxin

ActiveCN121380020BHydrolaseAmino acid
The application provides a vomitoxin hydrolytic enzyme and a method for degrading vomitoxin. The vomitoxin hydrolytic enzyme has an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence with more than 70% homology with SEQ ID NO: 1. The problem that the effect of the hydrolytic enzyme preparation for degrading vomitoxin in the prior art is poor in degrading DON can be solved, and the application is suitable for the field of degrading vomitoxin.
Owner:ANGEL YEAST CO LTD +1

Establishment of an animal model of poult proventriculitis induced by vomitoxin

The present application belongs to the technical field of disease model construction, and discloses establishment of a vomiting toxin-mediated gosling adenomygatitis animal model, and specifically discloses a method for constructing a chicken adenomygatitis model, which comprises the following steps: 0-day-old goslings are infected with a mixed solution of mycoplasma gallisepticum and escherichia coli, and then the goslings are fed with starter feed containing vomiting toxin and full-price feed containing vomiting toxin, so that the chicken adenomygatitis model is constructed. The method for constructing the chicken adenomygatitis model is simple and fast, the constructed model has obvious clinical symptoms and typical autopsy characteristics, is suitable for research on drugs for preventing and treating chicken adenomygatitis, can be used for exploring the pathogenesis of chicken adenomygatitis, and can be used for screening and optimizing a treatment scheme, evaluating the effectiveness and safety of the treatment scheme, thereby providing an experimental basis for controlling the disease.
Owner:GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST

Feed additive for degrading vomitoxin and application thereof

The application relates to the technical field of feed additives, in particular to a feed additive for degrading vomitoxin and application, wherein the feed additive for degrading vomitoxin is composed of, in percentage by weight, Bacillus altitudinis G-1 bacterial liquid: 10-30%; solid carrier: 70-90%. Efficient and stress-resistant Bacillus altitudinis G-1 is obtained through screening, the fermentation liquor is mixed with the treated solid carrier, and low-temperature or spray drying is adopted for shaping. The strain can secrete specific degradation enzyme system to efficiently convert vomitoxin, and can maintain activity in processing and digestive tract environment by virtue of wide temperature range and wide pH tolerance characteristics; in combination with the protection and slow-release effect of the carrier, efficient degradation of vomitoxin and synchronous inhibition of mold growth are realized, and the problems of limited efficiency of existing degradation strains, unstable activity in complex environment and lack of mold regeneration inhibition capacity are solved.
Owner:HEBEI JIWEI MODERN BIOTECHNOLOGY CO LTD +1

Rapid electrochemical detection method for vomitoxin based on enzymatic electropolymerization signal

The invention discloses a rapid electrochemical detection method for vomitoxin based on an enzymatic electropolymerization signal. The rapid electrochemical detection method comprises the following steps: mixing a to-be-detected sample solution with enzyme-labeled vomitoxin with a preset concentration to form a competitive reaction system; incubating the working electrode in a competitive reaction system to obtain a target working electrode; after uncombined substances on the target working electrode are removed, the target working electrode is placed in a detection solution containing an electro-polymerization monomer and an enzyme-labeled substrate, and a polymer film is formed; the electrochemical signal change before and after the formation of the polymer film is measured by adopting an electrochemical method, and the vomitoxin concentration in the sample solution to be detected is quantitatively detected based on the electrochemical signal change. The method ingeniously fuses the advantages of high selectivity of molecular imprinting, enzyme catalysis signal amplification and signal conversion of in-situ electropolymerization, does not need to depend on biological antibodies, and has the remarkable advantages of high sensitivity, good stability, simplicity and convenience in operation, rapidness in detection and the like.
Owner:HUIZHOU DINGSHUO IND CO LTD

Method for detecting vomitoxin in noodle product

The invention relates to a method for detecting vomitoxin in a noodle product, and belongs to the technical field of food inspection and detection. The invention relates to a method for detecting vomitoxin in a noodle product. The method mainly utilizes thin layer chromatography for rapid screening. The method is simple, rapid, high in sensitivity and suitable for rapid preliminary screening of initial quality inspection in the noodle product processing procedure.
Owner:临沂市粮食质量检测中心