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90 results about "Vomitoxin" patented technology
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Vomitoxin, also known as deoxynivalenol (DON), is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale. The occurrence of deoxynivalenol is associated primarily with Fusarium graminearum (Gibberella zeae) and F. culmorum, both of which are important plant pathogens which cause fusarium head blight in wheat and gibberella or fusarium ear blight in corn. A direct relationship between the incidence of fusarium head blight and contamination of wheat with deoxynivalenol has been established. The incidence of fusarium head blight is strongly associated with moisture at the time of flowering (anthesis), and the timing of rainfall, rather than the amount, is the most critical factor. However, increased amount of moisture towards harvest time has been associated with lower amount of vomitoxin in wheat grain due to leaching of toxins. Furthermore, deoxynivalenol contents are significantly affected by the susceptibility of cultivars towards Fusarium species, previous crop, tillage practices, and fungicide use. It occurs abundantly in grains in Norway due to heavy rainfall.
Preparation method and application of bacterium-enzyme-magnetic nano-cluster composite detoxification agent
The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.
Owner:HENAN QIULE SEEDS TECH CO LTD
Aspergillus niger recombinant strain for degrading vomitoxin and application thereof
The invention discloses an aspergillus niger recombinant strain for degrading vomitoxin and application thereof, and belongs to the field of gene engineering. In order to solve the problem that a method for efficiently degrading vomitoxin is lacked in the prior art, the invention provides an aspergillus niger recombinant strain for degrading vomitoxin, the aspergillus niger strain for co-expressing FsTRI12 is taken as a recipient bacterium, a vomitoxin acetyltransferase gene segment is selected, an intracellular gene expression vector is constructed, and a homozygous aspergillus niger recombinant strain is screened. The aspergillus niger recombinant strain provided by the invention can be applied to degradation of vomitoxin, provides a new choice for effectively solving the vomitoxin pollution problem in cereal raw materials and feeds, reducing grain loss and guaranteeing food and feed safety, and has potential industrial application prospects.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Bacteroides ovatus L2 for relieving vomitoxin toxicity and application of bacteroides ovatus L2
The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Apparatus for dynamic degradation of cereal vomitoxin based on ozone circulation
The utility model relates to grain safety processing equipment especially based on the equipment of dynamic degradation grain vomit toxin of ozone circulation, this equipment contains vertical treatment tower (1), ozone gas device contains the middle part cloth gas pipe (17) of middle part arrangement in vertical treatment tower (1), and the middle part cloth gas pipe (17) contains ozone entrance and cloth gas pipe with hole, grain circulation flow device contains the lower screw conveyer (5) for transportation, bucket elevator (8) and upper screw conveyer (18), bucket elevator (8) is arranged in the side of vertical treatment tower (1), and upper screw conveyer (18) is located above vertical treatment tower (1), and lower screw conveyer (5) is located below vertical treatment tower (1), and lower screw conveyer (5), bucket elevator (8) and upper screw conveyer (18) and vertical treatment tower (1) are combined as a whole material conveying circulation link.
Owner:ZHEJIANG SHANNONG MODERN AGRICULTURAL SCIENCE & TECHNOLOGY DEVELOPMENT CO LTD +2
Bacillus subtilis, complex microbial inoculant and application of bacillus subtilis and complex microbial inoculant in degradation of mycotoxin
The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.
Owner:SHANDONG BAOLAI-LEELAI BIOENGINEERING CO LTD (CN)
Method for improving nutritional quality of wheat and reducing vomitoxin by combining cold plasma with lactobacillus fermentation
The invention discloses a method for improving the nutritional quality of wheat and reducing vomitoxin by combining cold plasma with lactic acid bacteria fermentation. The method comprises the following specific steps: (1) putting wheat grains polluted by DON into a reaction cavity of cold plasma equipment; (2) preparing a single or compound lactic acid bacteria starter; (3) mixing the wheat subjected to cold plasma treatment with a lactic acid bacteria leavening agent for fermentation; and (4) drying, grinding and sieving the fermented wheat to prepare the wheat flour meeting the standard. Through progressive cooperative treatment of cold plasma pretreatment and compound lactobacillus fermentation, the final degradation rate of DON reaches 91.04%, functional components (resistant starch reaches 13.71% and dietary fiber reaches 13.14%) are synchronously increased, and phytic acid (lowest to 0.42%) is greatly degraded to improve the bioavailability of mineral substances. Besides, the method can optimize the wheat processing performance, the prepared noodles are moderate in hardness, good in elasticity and stretch-proof, no chemical reagent is added in the whole process, collaborative optimization of efficient detoxification, nutrient enrichment and processing performance improvement is achieved, and a green and economical solution is provided for safe utilization of polluted wheat.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
DDS pulp capable of degrading vomitoxin and glycerol and preparation method of DDS pulp
The invention relates to the technical field of deep processing of agricultural and sideline products and feed additives, in particular to DDS pulp capable of degrading vomitoxin and glycerol and a preparation method of the DDS pulp. According to the method, the DDS pulp is inoculated with the specifically screened complex microbial inoculants, liquid fermentation is carried out under proper conditions, microorganisms preferentially utilize free glycerol in the DDS pulp as a carbon source for metabolism, the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the method, when the complex microbial inoculant is inoculated, the DDS pulp is inoculated with a functional strain with a vomitoxin degradation function and a special enzyme preparation, the functional strain is colonized in a system and continuously produces enzyme, and the added enzyme preparation can quickly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the degradation efficiency of the vomitoxin is improved. And the degradation product is converted into a non-toxic or low-toxicity degradation product.
Owner:HAILUN BAIZUN BIOENGINEERING CO LTD +1
A labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection
The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.
Owner:GUANGZHOU INST FOR FOOD INSPECTION(GUANGZHOU INSPECTION CENT FOR WINE & SPIRITS)
Microbial composition for preventing and treating vomitoxin as well as preparation method and application of microbial composition
The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.
Owner:CHENGDU HUAHONG ECOLOGICAL AGRI TECH CO LTD
Application of modified montmorillonite in the adsorption of mycotoxins
The present invention discloses a modified montmorillonite for use in mycotoxin adsorption. The invention utilizes a specific organic solvent for thermal swelling and exfoliation treatment of the montmorillonite, exposing more adsorption active sites on the montmorillonite's two-dimensional surface. This increases the specific surface area of the montmorillonite nanosheets and enhances their adsorption capacity for weakly polar compounds. Combined with thermal activation and ultrasonic treatment, the modified montmorillonite further enhances its surface area, ultra-thin montmorillonite nanosheets, and a high proportion of coordinatively unsaturated surface sites. This increased number of active sites enables the modified montmorillonite to exhibit strong adsorption capacity in mycotoxin adsorption applications, particularly significantly improving adsorption efficiency for weakly polar mycotoxins such as zearalenone and vomitoxin by at least 93.17% and 49.31%, respectively, compared to unmodified sodium montmorillonite.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Efficient degradation method for mycotoxin in corn steep liquor
The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor, and belongs to the technical field of corn deep processing and detoxification. The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor. The method comprises the following steps: adjusting the pH value of thick corn steep liquor by using an alkaline solution; adding a solid catalyst into the corn steep liquor; carrying out detoxification treatment by combining heterogeneous ozone oxidation reaction with microbubbles; and separating and recovering the solid catalyst to obtain the detoxified corn steep liquor. The vomitoxin content of the treated concentrated corn pulp is smaller than 1000 g / kg, the degradation rate is larger than 93%, the zearalenone toxin content is smaller than 33 g / kg, and most of the zearalenone toxin is removed. According to the method disclosed by the invention, the content of fungaltoxin in the corn steep liquor is reduced, so that the economic value of corn byproducts is improved, the economic benefit of grain trade is favorably improved, and the storage period and value of produced products are improved.
Owner:SHANDONG FUYANG BIO-TECH CO LTD
Devosia sp. D-G15 and application thereof
The application isolates a new strain of Devosia genus bacteria from the soil of wheat field infected by Fusarium, and names it as Devosia sp. D-G15 (D-G15). The ANI value of D-G15 and other strains in the Devosia genus is less than 80%, which indicates that it is a new species of Devosia strain. The strain has been preserved in Guangdong Microbial Culture Collection Center on March 26, 2024, and the preservation number is GDMCC NO: 64453. The strain D-G15 can degrade vomitoxin (DON) into low-toxicity products, and the degradation of the strain to DON is beneficial within the range of pH value of 4-10 and temperature of 15-35 DEG C. The degradation activity of D-G15 is stable, the metabolic capacity is strong, and it is safe in the animal body, and can be used for the preparation of DON detoxification preparation and applied to the fields of feed processing and food processing.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Composite mildew removing agent as well as preparation method and application thereof
The invention relates to a composite mildew removing agent as well as a preparation method and application thereof, relates to the technical field of mildew removing agents, and aims at solving at least one of the problems that an existing mildew removing agent is low in vomitoxin and zearalenone adsorption rate, low in degradation capacity, high in cost, unstable in performance and the like. The composite mildew removing agent is prepared from the following raw materials in percentage by mass: 30 to 35 percent of calcium-based montmorillonite, 32 to 42.5 percent of sodium-based montmorillonite, 10 to 15 percent of zinc-loaded montmorillonite, 10 to 15 percent of yeast cell wall, 2 to 5 percent of L2-ascorbic acid, 1 to 2 percent of chitosan oligosaccharide, 0.5 to 1 percent of lactobacillus plantarum and 0.0005 to 0.002 percent of mangiferin. According to the composite mildew removing agent, the calcium-based montmorillonite, the sodium-based montmorillonite and the zinc-loaded montmorillonite have a synergistic effect, so that a multi-stage adsorption network is formed, the space barrier effect is enhanced, two toxins DON and ZEN in feed can be effectively removed, the loss that the feed cannot be used or needs to be degraded for use due to toxin pollution is reduced, the utilization rate of the feed is increased, and the feed quality is improved. The production cost of feed enterprises is reduced.
Owner:BEIJING TAOGUTU NEW MATERIAL CO LTD
Use of armillaria in degrading vomitoxin
The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.
Owner:JILIN AGRICULTURAL UNIV
Vomitoxin detoxification enzyme DH9-2 as well as coding gene and application thereof
The invention discloses a vomitoxin detoxification enzyme DH9-2 derived from Devosia sp. SD9-2 previously separated from yellow mealworm excrement, the amino acid sequence of the vomitoxin detoxification enzyme DH9-2 is shown as SEQ ID NO: 2 in a sequence table, and the nucleotide sequence of the coding gene of the vomitoxin detoxification enzyme DH9-2 is shown as SEQ ID NO: 1 in the sequence table. The invention also discloses a recombinant expression vector containing the coding gene, a recombinant host cell transformed with the vector, and a method for producing the detoxification enzyme by using the host cell. The detoxification enzyme can efficiently oxidize vomitoxin into a metabolite 3-keto-vomitoxin with low toxicity by depending on the existence of Ca < 2 + > ions and PQQ coenzyme under the conditions that the temperature is 35 DEG C and the pH value is 9.0, and the degradation rate of 50ug / mL DON in vitro can reach 100%. The invention particularly discloses application of the enzyme to degradation of vomitoxin in tenebrio molitor wheat bran feed and the like, and a new and effective biotechnology tool is provided for solving the problem of DON pollution in tenebrio molitor breeding and guaranteeing feed safety and tenebrio molitor health.
Owner:宁夏回族自治区粮油产品质量检测中心 +1
A method for constructing a yeast surface display of a two-enzyme system and its application in cascade detoxification of vomitoxin
The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.
Owner:ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
Quality safety evaluation method for non-grain type protein source feed
PendingCN122022567AChemical property predictionChemical analysis using combustionNutritionAmino acid composition
The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.
Owner:NINGXIA UNIVERSITY +1
Application of Fuzhuan tea extract in prevention of piglet health injury caused by vomitoxin
The invention belongs to the technical field of animal nutrition and feed, discloses an application of a Fuzhuan tea extract in prevention of vomitoxin-caused piglet health injury, and particularly relates to an application of the Fuzhuan tea extract as a feed additive in prevention or improvement of piglet health injury. The feed additive can effectively reduce negative effects on weaned piglets caused by excessive vomitoxin in the feed, wherein the negative effects comprise growth performance reduction, diarrhea rate increase, intestinal morphology damage, intestinal cell proliferation inhibition, intestinal epithelial barrier injury, immune disorder, abnormal liver function and excessive oxidative stress and inflammatory response.
Owner:HUNAN AGRI UNIV +1
Silage additive for degrading mycotoxin and application thereof
The invention relates to the technical field of silage, and provides a silage additive for degrading mycotoxin and application of the silage additive. The composite microbial inoculant is composed of lactobacillus paracasei and bacillus subtilis; or the microbial agent is a composite microbial agent composed of lactobacillus paracasei, bacillus subtilis and pediococcus pentosaceus. By screening degrading bacteria aiming at ochratoxin, aflatoxin, zearalenone and vomitoxin for compounding and combination, the degrading effects of different lactic acid bacteria on various toxins are respectively exerted, the various mycotoxins are effectively degraded, and particularly, the degrading rate of the ochratoxin can reach 100%. The degradation rate of the mixed bacteria to other mycotoxins can exceed that reported in the prior art, and faster degradation or higher degradation rate can be achieved by adjusting the addition amount.
Owner:CHINA AGRI UNIV
A vomitoxin detoxification enzyme, its encoding gene, and its applications
This invention discloses a vomitoxin detoxification enzyme, its encoding gene, and its applications, belonging to the field of molecular biology. This invention discovered a wheat SOT gene, TaSOT2b. The TaSOT2b protein was obtained through in vitro induced expression, and its amino acid sequence is shown in SEQ ID NO.1. This invention found that this protein possesses in vitro enzymatic activity, capable of modifying DON into SON-3-sulfate, and can act as a vomitoxin detoxification enzyme. It has potential application value and reference significance for the scientific prevention and control of wheat scab and DON toxin contamination, and has broad application prospects in agriculture, feed, and industry.
Owner:山东国仓健生物科技有限公司
Application of glucuronolactone in the preparation of feed to alleviate spleen damage caused by combined PRRSV and vomitoxin
This invention belongs to the field of animal feed technology and discloses the application of glucuronolactone in the preparation of feed that alleviates spleen damage caused by the combined effects of PRRSV and DON. This invention discovers that glucuronolactone can effectively alleviate spleen damage in piglets caused by co-exposure to PRRSV and DON. It exerts its protective effect by activating the Nrf2 antioxidant signaling pathway, improving spleen tissue structure, alleviating oxidative stress, inhibiting inflammatory responses, and inhibiting apoptosis. This invention also provides a feed containing the aforementioned effective amount of glucuronolactone, with the basic feed tailored to the nutritional needs of piglets. This invention fills the technological gap in effective intervention methods for spleen damage under PRRSV and DON co-exposure. The glucuronolactone addition process is simple, the effect is significant, and it is suitable for large-scale feed production. It can effectively solve the problem of immunosuppression in pig herds caused by the combined harm of PRRSV and DON in the pig industry, and has important practical value.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Treatment system for reducing toxin content in corn water-soluble protein
ActiveCN223646423UWater/sewage treatment by irradiationFood processingAgricultural sciencePulp and paper industry
The utility model relates to the technical field of corn soaking water treatment, in particular to a treatment system for reducing toxin content in corn water-soluble protein, which comprises a first filtering device communicated with a corn soaking water conveying pipeline, and a second filtering device communicated with a filtrate outlet of the first filtering device. A liquid outlet of the second filtering device is communicated with toxin adsorption equipment, a liquid outlet of the toxin adsorption equipment is communicated with a concentration device, a concentrated solution outlet of the concentration device is communicated with a third filtering device, a liquid outlet of the third filtering device is communicated with a drying device, and a discharge port of the drying device is communicated with a product storage tank. The treatment system is reasonable in design, the content of vomitoxin and fumonisins in the protein product can be effectively reduced through the toxin adsorption equipment, and the safety of the protein product is guaranteed.
Owner:ZHUCHENG HAOTIAN PHARMA CO LTD
Bacillus velezensis ATC-AL and application thereof
The invention discloses a bacillus velezensis ATC-AL and application thereof. The preservation number of the strain is CCTCC (China Center For Type Culture Collection): M20251280. A genome of the bacillus velezensis ATC-AL has a synthetic gene cluster for synthesizing various antifungal active substances, the active substances of the bacillus velezensis ATC-AL can destroy cell surface structures of pathogenic bacteria, induce cell apoptosis and inhibit spore development of pathogenic fungi, and the bacillus velezensis ATC-AL has a broad-spectrum inhibition effect on various pathogenic fungi such as sweet potato coracocystis canaliculata, fusarium graminearum and aspergillus flavus; the generation inhibition rate of vomitoxin reaches 95%, and the generation inhibition rate of aflatoxin B1 can reach 100%; fungus infection experiments on sweet potatoes, corn and wheat prove that active substances in a strain cell fermentation broth can completely inhibit the decay effect of sweet potato coracocystis longirostris, fusarium graminearum and aspergillus flavus on the food crops; according to the invention, a microbial control resource library for biological control of pathogenic fungi and toxin pollution thereof is enriched.
Owner:XUZHOU NORMAL UNIVERSITY
Construction method and application of SERS (Surface Enhanced Raman Scattering) biosensor for detecting vomitoxin
PendingCN121954835AGood Raman enhancement effectImprove hydrophobicityRaman scatteringBiological testingChemical physicsSignalling molecules
The invention provides a construction method and application of an SERS (Surface Enhanced Raman Scattering) biosensor for detecting vomitoxin. The SERS biosensor is used for rapidly detecting the vomitoxin (DON) in real time. Ag is sputtered on the surface of the filter paper through a magnetron sputtering method, so that the surface of the filter paper has a Raman signal enhancement effect. An aptamer (Apt) of vomitoxin is selected as a recognition sensing element to specifically capture DON, Apt is connected to filter paper through an Ag-S bond, Au-4-ATP-cDNA is further modified and connected to the filter paper through complementation of Apt and cDNA, and 4-ATP serves as a signal molecule to provide a Raman signal. When the DON exists, the Apt is combined with the DON and is separated from the cDNA, the Raman signal of the 4-ATP is weakened after the Au-4-ATP-cDNA falls off, and the DON is quantitatively analyzed by detecting the Raman signal change of the 4-ATP. The sensor has high sensitivity, specificity and stability, provides a new idea for on-site rapid detection and real-time monitoring, and has application value and application prospect.
Owner:NANJING NORMAL UNIVERSITY
Composition for relieving vomitoxin harm of weaned piglets as well as preparation method and application of composition
The invention relates to the technical field of breeding, in particular to a composition for relieving vomitoxin harm of weaned piglets and a preparation method and application of the composition. The composition is prepared from the following components in parts by weight: 50 to 70 parts of glucurolactone, 10 to 15 parts of cynarin, 20 to 40 parts of scutellaria baicalensis flavone, 10 to 20 parts of stevia rebaudiana extract and 80 to 120 parts of a carrier. The composition can be used for effectively relieving the symptoms of diarrhea, weight loss and intestinal dysfunction of weaned piglets.
Owner:ZHUCHENG HAOTIAN PHARMA CO LTD
Vomitoxin hydrolase and method for degrading vomitoxin
The invention provides a vomitoxin hydrolase and a method for degrading vomitoxin. The vomitoxin hydrolase has an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence having more than 70% of homology with the SEQ ID NO: 1. The problem that in the prior art, a hydrolase preparation for degrading vomitoxin is poor in DON degradation effect can be solved, and the hydrolase preparation is suitable for the field of vomitoxin degradation.
Owner:ANGEL YEAST CO LTD +1
Vomitoxin hapten, vomitoxin antigen, vomitoxin antibody, preparation methods of vomitoxin hapten, vomitoxin antigen and vomitoxin antibody, and light-activated chemiluminescence
The invention relates to the technical field of immunoassay, and provides a vomitoxin hapten, an antigen, an antibody, a preparation method and a light-activated chemiluminescence quantitative detection kit. The hapten is obtained by adopting cyclic dicarboxylic anhydride as a reactant and introducing an active group on a C atom of conjugated alkene of vomitoxin molecules through Friedel-Crafts acylation reaction. After the hapten is coupled with a carrier protein, vomitoxin molecules can be fully exposed outside the carrier protein, the immunogenicity of the antigen is enhanced, and an antibody with high titer and high sensitivity can be generated after the hapten is immunized by a host animal. Results of the embodiment show that after the vomitoxin antigen is subjected to mouse injection immunization, the titer of the generated antibody is 1: 20000, and the half inhibitory concentration (IC50) is less than 50ppb / mL. When the obtained vomitoxin antigen and antibody are applied to rapid quantitative detection of light-activated chemiluminescence of vomitoxin, the detection limit, the false negative rate and other dimensions are qualified, the detection steps are simple, and the vomitoxin antigen and antibody have good industrial value.
Owner:CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT +1
Efficient deoxynivalenol degrading enzyme mutant and application thereof
The invention discloses a sorbose dehydrogenase mutant capable of efficiently degrading vomitoxin, and belongs to the technical field of agricultural biology. The mutant is based on wild type SDH, through rational design and a directed evolution strategy, the mutant comprises three mutation sites F103A, T492E and N427A, the amino acid sequence and the nucleotide sequence of the mutant are SEQ ID NO.1 and SEQ ID NO.2 respectively, the mutant SDHF103A / T492E / N427A can efficiently degrade DON without depending on phenazine methyl sulfate (PMS), and the degradation activity of the mutant SDHF103A / T492E is improved by about two times compared with that of a double mutant SDHF103A / T492E under the condition that PMS does not exist. The method can be applied to preparation of the vomitoxin detoxification enzyme, and has a good application prospect in the aspect of biological detoxification of DON in feeds, grains and food.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Dvorstia and method for fermenting corn steep liquor using the same
The present application relates to the technical field of microorganism, and particularly relates to a Dvorst's bacteria and a method for fermenting corn steep liquor by using the same.The present application provides a Dvorst's bacteria capable of efficiently degrading vomitoxin in corn steep liquor, and corn steep liquor is fermented by using the Dvorst's bacteria and Candida krusei in cooperation, so that the pH of the corn steep liquor can be increased, the content of vomitoxin and anti-nutritional factors in the corn steep liquor can be reduced, and the content of choline in the corn steep liquor can be significantly increased, the quality of the corn steep liquor is obviously improved, the corn steep liquor after the cooperative fermentation can be better applied in the field of animal feed, and has a wide application prospect.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
A method of targeted degradation of vomitoxin in feed and a fusion enzyme
ActiveCN117814431BPolypeptide with localisation/targeting motifFood processingSingle-Chain AntibodiesAntiendomysial antibodies
The application belongs to the technical field of biotechnology, and particularly relates to a method for targeted degradation of vomitoxin in feed and a fusion enzyme. The application fuses and expresses a DON degrading enzyme, i.e. a manganese peroxidase, with a single-chain antibody capable of specifically recognizing DON, and the obtained fusion enzyme can efficiently target and degrade vomitoxin in feed, can efficiently degrade mycotoxins, has low cost, and has wide application range, and can be widely used in the field of feed toxin degrading enzymes.
Owner:INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES