282 results about "Penicillium" patented technology

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The name of the invention is application of Peniazathilone A as an alpha-glucosidase inhibitor and a preparation method of the Peniazathilone A. The invention belongs to the technical field of medicines, and relates to application of an azone compound as the alpha-glucosidase inhibitor. The compound has the following structure: # imgabs0 #. The compound Peniazathilone A is obtained by extracting, separating and purifying an ethyl acetate extract phase of a fermented product of Penicillium sp from soil, and the alpha-glucosidase inhibition activity of the compound is evaluated by adopting a pNPG method. Experimental results show that the IC50 value of the compound Peniazathilone A is 86.8 [mu] M (the IC50 value of a positive control acarbose is 5.97 mM), and the inhibition rate reaches 92.4% when the concentration of the compound is 0.466 mM. The compound has obvious activity of inhibiting alpha-glucosidase, is expected to be developed into a novel hypoglycemic drug, and has potential medical and health-care values.

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention provides a Penicillium camponotum bacterium and an application of the Penicillium camponotum bacterium in fermentation of agilawood for aroma enhancement. According to the invention, a strain of Penicillium camponotums is obtained by screening from a healthy agilawood tree, and the strain of Penicillium camponotums is named as Penicillium camponotums CX-3, and the strain of Penicillium camponotums is named as Penicillium camponotums CX-3. After fermentation liquor of the strain is added into an agilawood raw material for fermentation, the content of active substances can be increased, the fermentation time has important influence on the content of agilawood alcohol extract, the content of agilawood characteristic substances and the increase of agilawood alcohol extraction antioxidant activity, and it is proved that the strain has wide application prospects in industrial production of agilawood.

The invention discloses a hermetia illucens breeding method for improving the biotransformation capacity of hermetia illucens on agricultural wastes. The hermetia illucens breeding method capable of improving the biotransformation capacity of the hermetia illucens on the agricultural waste comprises the following steps: inoculating larvae of the hermetia illucens into the agricultural waste added with Penicillium nucleatum, breeding, and collecting insect sand and insect bodies. The method disclosed by the invention can be used for improving the biotransformation capability of the hermetia illucens on the agricultural wastes, which is specifically embodied in the aspects of increasing the waste reduction rate, increasing the biotransformation rate, increasing the biomass transformation ratio of the substrate and the hermetia illucens prepupae, increasing the growth rate of the hermetia illucens, increasing the dry matter content and the protein content of hermetia illucens bodies and the like; the method provided by the invention can realize rapid and efficient degradation of wastes, reduces resource loss and environmental pollution, provides a brand new solution for waste treatment, and comprehensively improves the resource utilization efficiency.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

The invention discloses a microbial image recognition method and system based on a dual-input convolutional neural network, and relates to the technical field of microbial image recognition, and the method comprises the steps: 1, collecting a microbial sample image, and carrying out the preprocessing of the sample image; 2, constructing a data set according to the preprocessed sample images; 3, inputting the data set into a backbone network of the double-input convolutional neural network for feature extraction, and forming an independent feature vector; 4, fusing the feature vectors, and introducing an attention mechanism module for optimization to obtain an optimized fused feature vector; and 5, performing classification decision according to the fusion feature vector, and outputting a microbial image recognition classification result. According to the invention, two types of images are used as independent inputs for deep fusion, and morphological features of different levels are complementarily extracted; and the distinguishing capability can be obviously improved in distinguishing morphological similar species (such as penicillium and aspergillus).

The invention relates to the technical field of microorganisms, in particular to Penicillium poplar RS4.5 and application thereof in plant salt resistance. The preservation number of the penicillium poplar RS4.5 is CGMCC (China General Microbiological Culture Collection Center) NO. 3.27774; tests prove that the strain (Penicillium poplar RS4.5) can obviously promote the growth of sainfoin and can also obviously promote the growth of sainfoin under saline-alkali stress, so that the strain has an application prospect in the aspects of improving the stress resistance of plants and the like.

The invention provides an application of a penicillium strain in growth promotion and antibiosis, and particularly relates to an application of a penicillium strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.12767 in growth promotion and antibiosis. The penicillium strain can promote crop growth in Guangdong, Heilongjiang, Shandong and fields, can promote crop growth in fields and prevent and treat eubacterial diseases, is compatible with chemical drugs, can be suitable for soil in different areas and different environments to promote crop growth, and can prevent and treat eubacterial diseases.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of biological control, in particular to a penicillium strain F5 and application thereof. The invention provides a Penicilliumsp. Strain F5, and the preservation number of the strain is GDMCC No: 65364. The invention also provides a preparation method of the Penicilliumsp. Strain F5. The invention further provides application of the penicillium strain F5 in preventing and treating plant wilt caused by fusarium oxysporum and tobacco black shank caused by phytophthora nicotianae. The penicillium strain F5 not only can obviously inhibit the growth of fusarium oxysporum and phytophthora nicotianae, but also can effectively promote the growth of plants, and has a good application prospect in the prevention and treatment of plant diseases.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The invention relates to the field of environment and agricultural biotechnology, in particular to a phosphorus-solubilizing fungus, a phosphorus-solubilizing microbial inoculum and application thereof.The classification name of a phosphorus-solubilizing fungus strain XF2 is Penicillium deserticola, the strain XF2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number is CGMCC NO: 41748. The bacterial colony characteristic of the strain XF2 is round, the edge is in a winding texture fluffy shape, the bacterial colony is yellow green or grey green, the surface is relatively smooth, and the bacterial colony can grow in a feather shape or a radial shape. Besides, after the strain XF2 is subjected to culture condition optimization, the phosphorus solubilizing amount can reach 874.3 mg / L. Therefore, the phosphorus solubilizing fungus has the advantage of high phosphorus solubilizing capacity, can be used for preparing a phosphorus solubilizing bacterial agent, has a very good application prospect, and particularly has a good application prospect in the aspects of dissolving phosphate ore and developing a phosphorus solubilizing fungus fertilizer.

The invention relates to the technical field of microorganisms, in particular to penicillium LDFST10 and application of the penicillium LDFST10 in promoting plant growth or preventing and treating stem rot. The preservation number of the penicillium LDFST10 is CGMCC (China General Microbiological Culture Collection Center) No. 41960. The application comprises the step of applying the penicillium LDFST10 or the fermentation product thereof to rhizosphere soil of the plant so as to promote the growth of the plant and prevent and treat basal stem rot. The invention researches and screens out the penicillium frutescens LDFST10 capable of promoting plant growth and inhibiting various pathogenic bacteria of stem rot, various growth promoting preparations or bacteriostatic agents or drugs aiming at the stem rot can be developed on the basis of the penicillium frutescens, and the penicillium frutescens LDFST10 has important value in the planting field of various crops such as corn, wheat and the like.

The invention relates to the discovery of a biosynthetic gene cluster of an alkaloid, namely, exopoid C, and the biosynthetic gene cluster of the exopoid. A biosynthetic gene cluster exo of an exopoid compound is obtained from a fungus Penicillium sp.HDN14-431 through genome mining, the gene cluster comprises a heme-dependent oxidase gene exoA which is composed of indole dioxygenase and a cytochrome b5 structural domain, a cytochrome P450 enzyme gene exoB and an isopentenyl transferase gene exoC which is composed of indole dioxygenase and a cytochrome b5 structural domain, and the cytochrome P450 enzyme gene exoB and the isopentenyl transferase gene exoC are used as biosynthetic genes of the exopoid compound. All genes in a gene cluster are expressed through an aspergillus nidulans heterologous expression system, two isopentenyl indole alkaloids are obtained in total, and a compound I is a new compound exopoid C. And the compound II, namely the exopoid, is a known compound. The biosynthesis pathway of the compound is reported for the first time.

The invention relates to a preparation method and application of antibacterial and starch aging resistant biphase gel, and belongs to the field of food processing. The preparation method is characterized by comprising the following steps: preparing oleogel from monoglyceride, corn oil and curcumin, preparing hydrogel from wheat bran araboxylan and tea polyphenol, mixing the oleogel and the hydrogel, adding lecithin, and homogenizing at a high speed to obtain the gel with a two-phase system. The biphase gel produced by the invention has the characteristics of zero trans-fat, high fiber content, simple preparation method and the like, realizes synergistic stability of oil and water phases, has both the mechanical strength of oil gel and the biocompatibility of hydrogel, can replace shortening in baked food, and has a wide application prospect. And the shelf life of the baked product is prolonged through the synergistic effect of antibiosis and starch aging resistance. The mass ratio of the oil phase to the water phase in the biphase gel is 1: 3-3: 1, the addition amount is 10-20% of the mass of the flour, the bacteriostasis rates on penicillium and rhizopus are 60-85% and 65-90% respectively, the starch crystallinity in the bread storage process is reduced by 10-20%, and the shelf life of the bread is prolonged by 10-20 days.

The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacterium bacillus velezensis SJD33 and application thereof. The preservation number of the bacillus velezensis SJD33 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.29456. The bacillus velezensis SJD33 provided by the invention has the advantages that the preservation number is CGMCC No.29456; the strain has a remarkable inhibition effect on pear fruit rot pathogens (physalospora piricola, penicillium, alternaria alternata, athelia fruit rot pathogens, botrytis cinerea and colletotrichum gloeosporioides) and other fruit pathogenic bacteria (apple core pathogens, brown rot pathogens and pomegranate dry rot pathogens). The SJD33 can obviously reduce the natural rotting rate of the pear fruits; the SJD33 can be rapidly colonized in fruit wounds; the SJD33 does not influence the fruit quality and can delay the reduction of the fruit hardness and the L-ascorbic acid; the SJD33 secondary metabolite is stable in activity and has great application potential. The strain provides a new strain resource for a fruit disease biocontrol resource library.

The invention belongs to the field of microorganisms, and particularly provides a non-pathogenic penicillium ochrochloron strain which is derived from garlic rhizosphere soil, is classified and identified as penicillium ochrochloron, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC 41151. The strain has broad-spectrum bacteriostatic activity and can antagonize various plant pathogens; the plant growth promoting agent has the growth promoting characteristic, can secrete siderophores and phospholytic enzymes, and improves plant iron and phosphorus nutrition; the composition is safe and free of pathogenicity to crops. Tests show that the P4 strain can relieve plant iron deficiency yellowing and promote crop growth. The invention also provides application of the strain in biological control of plant diseases and plant growth promotion.

The present invention generally relates to an isolated Penicillium strain, named Penicillium olsonii TLL1, with a preservation number of NRRL 68252, and to the use thereof in promoting plant growth, and to the use of the Penicillium strain, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1. Specifically, the present invention provides compositions suitable for promoting plant growth comprising the isolated fungal strains that can mitigate Al toxicity 5 and increase phosphate effectiveness, as well as methods of their promotion of plant growth.

The invention discloses a strawberry and grape fresh-keeping composite coating film, a preparation method and application, the fresh-keeping composite coating film mainly comprises chitosan, oxidized starch, phytic acid and carvacrol, the rotting rate of strawberries and grapes is reduced, the chromaticity and hardness of the strawberries and the grapes are maintained, the aging of the strawberries and the grapes is delayed, and the fresh-keeping effect of the strawberries and the grapes is improved. The growth of rot-causing fungi alternaria alternata and Penicillium Orsenii in strawberries and grapes is inhibited, the quality of strawberry and grape fruits can be maintained, and the storage period of the strawberries and the grapes can be prolonged.

The invention relates to a preparation method of a plant disease control fungicide, which comprises the following steps: taking traditional Chinese medicines with an antibacterial effect as raw materials, and carrying out first-step fermentation by adopting Trichoderma harzianum; beauveria bassiana and Penicillium baylii are subjected to second-step fermentation, bacillus velezensis is subjected to third-step fermentation, fermentation liquor is collected, a filter cake is further subjected to reflux extraction, an extracting solution and the fermentation liquor are collected, combined and concentrated into clear paste, an ethanol solution is added, standing is carried out, supernate is taken, borneol, gelatin and cellulose acetate are added to prepare mixed liquor, ultrafiltration is carried out, ultrafiltrate is collected, and freeze-dried powder is obtained. The microbial inoculum required by the invention is obtained. According to the invention, specific traditional Chinese medicines are adopted, an excellent broad-spectrum antibacterial system is formed through specific microbial fermentation, and the antibacterial system is compounded with borneol, gelatin, cellulose acetate and other components to form the fungicide, so that the effectiveness duration and the effect taking rate of the fungicide on the surface of a plant are effectively improved, and efficient prevention and treatment on various diseases such as fungi, bacteria and the like are formed.

The invention discloses a sandy soil conditioner and application thereof. The sandy soil conditioner is prepared from the following raw materials in parts by mass: 1 to 5 parts of sodium carboxymethyl cellulose, 100 to 126 parts of biochar, 15 to 30 parts of humic acid and 0.5 to 1.5 parts of microbial agent. The microbial agent is prepared from bacillus subtilis and penicillium baylii according to the mass ratio of (1 to 3): (0.5 to 1.5). According to the invention, sodium carboxymethyl cellulose, biochar, humic acid and a specific microbial agent are scientifically proportioned, the addition of biochar significantly enhances the water and fertilizer retention ability of soil, the porous structure effectively adsorbs and retains a large amount of water and nutrients, the nutrient loss is reduced, and the soil fertility is improved; the humic acid is rich in organic matters and various nutrient elements, so that the organic matter content of the soil is effectively increased, the soil structure is improved, a stable granular structure is formed, and the air permeability and the water-retaining property of the soil are enhanced; sodium carboxymethyl cellulose is used as a soil conditioner, so that the agglomeration and the stability of the soil are further enhanced.

The invention relates to the technical field of agricultural microorganisms, and discloses a complex microbial inoculant for improving soil micro-ecology and promoting plant growth and application thereof.The complex microbial inoculant is prepared from 30-40 parts of bacillus subtilis, 20-40 parts of pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of trichoderma and 5-10 parts of penicillium; wherein the bacillus subtilis is bacillus subtilis L11 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.31274, the preservation date is July 11, 2024, and the bacillus subtilis is classified and named as bacillus subtilis. The complex microbial inoculant for improving the soil micro-ecology and promoting the plant growth is adopted, a multi-bacterium synergistic community is constructed, the complementary metabolism advantage of different strains at the rhizosphere is utilized, the nutrient conversion and stress resistance characteristics are improved, and therefore the soil micro-ecology is improved, and the plant root development is promoted.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention discloses a penicillium oxalicum strain and application thereof. The strain is named as Penicillium oxalicum HYC2101, the Penicillium oxalicum HYC2101 is registered and preserved in the China General Microbiological Culture Collection Center on August 3, 2022, the preservation number is CGMCC No.40263, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also discloses application of the strain in prevention and treatment of plant pests and preparation of a citrus pathogenic fungus inhibitor.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention belongs to the technical field of agricultural production, and particularly relates to a biochar-based fertilizer for promoting yield increase of pepper and a preparation method thereof.The biochar-based fertilizer is prepared from, by weight, 700-800 kg of biochar, 100-150 parts of urea, 100-150 parts of potassium sulfate, 2-4 parts of bio-enzyme and 3-5 parts of functional microbial flora; the biological enzyme comprises polypeptidase and protease; the functional microorganism bacteria comprise bacillus subtilis, trichoderma harzianum and penicillium wuelyi. In the pepper planting process, 10%-20% of a conventional chemical fertilizer is replaced with the charcoal-based fertilizer, the plant height, the plant width and the stem height can be remarkably increased, and the pepper yield is increased; experiments show that the functional microbial flora and the biological enzyme in the fertilizer have a synergistic effect, the preparation method is simple, the yield of the pepper is obviously increased, and the fertilizer is suitable for large-scale popularization and application and wide in market prospect.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.