219 results about "Penicillium" patented technology

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention provides a Penicillium camponotum bacterium and an application of the Penicillium camponotum bacterium in fermentation of agilawood for aroma enhancement. According to the invention, a strain of Penicillium camponotums is obtained by screening from a healthy agilawood tree, and the strain of Penicillium camponotums is named as Penicillium camponotums CX-3, and the strain of Penicillium camponotums is named as Penicillium camponotums CX-3. After fermentation liquor of the strain is added into an agilawood raw material for fermentation, the content of active substances can be increased, the fermentation time has important influence on the content of agilawood alcohol extract, the content of agilawood characteristic substances and the increase of agilawood alcohol extraction antioxidant activity, and it is proved that the strain has wide application prospects in industrial production of agilawood.

The embodiment of the invention discloses a Yarrowia guilliermondii as well as a separation and screening method, an identification method and application of the Yarrowia guilliermondii. The preservation number of the Yarrowia guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No.27603, and the preservation number of the Yarrowia guilliermondii is CGMCC No.27603. The Yarrowia guilliermondii disclosed by the embodiment of the invention has a very good prevention and control effect on postharvest diseases of fruits, and the prevention and control effect of the Yarrowia guilliermondii on the diseases can be enhanced by respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia yeast. Through identification, the antagonistic bacterium is a Yeyrozyma guilliermondii, and the antagonistic bacterium is a Yeyrozyma guilliermondii. The M.guilliermonii has a good control effect on gray mold, penicilliosis, black spot and aspergillus niger of strawberry, apple, pear and tomato fruits, can be rapidly colonized on fruit wounds, and can enhance the resistance-related enzyme activity of the fruits, occupy the wound space and induce the fruit resistance to reduce the morbidity of diseases.

The invention discloses a microbial image recognition method and system based on a dual-input convolutional neural network, and relates to the technical field of microbial image recognition, and the method comprises the steps: 1, collecting a microbial sample image, and carrying out the preprocessing of the sample image; 2, constructing a data set according to the preprocessed sample images; 3, inputting the data set into a backbone network of the double-input convolutional neural network for feature extraction, and forming an independent feature vector; 4, fusing the feature vectors, and introducing an attention mechanism module for optimization to obtain an optimized fused feature vector; and 5, performing classification decision according to the fusion feature vector, and outputting a microbial image recognition classification result. According to the invention, two types of images are used as independent inputs for deep fusion, and morphological features of different levels are complementarily extracted; and the distinguishing capability can be obviously improved in distinguishing morphological similar species (such as penicillium and aspergillus).

The invention relates to the technical field of microorganisms, in particular to Penicillium poplar RS4.5 and application thereof in plant salt resistance. The preservation number of the penicillium poplar RS4.5 is CGMCC (China General Microbiological Culture Collection Center) NO. 3.27774; tests prove that the strain (Penicillium poplar RS4.5) can obviously promote the growth of sainfoin and can also obviously promote the growth of sainfoin under saline-alkali stress, so that the strain has an application prospect in the aspects of improving the stress resistance of plants and the like.

The invention provides an application of a penicillium strain in growth promotion and antibiosis, and particularly relates to an application of a penicillium strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.12767 in growth promotion and antibiosis. The penicillium strain can promote crop growth in Guangdong, Heilongjiang, Shandong and fields, can promote crop growth in fields and prevent and treat eubacterial diseases, is compatible with chemical drugs, can be suitable for soil in different areas and different environments to promote crop growth, and can prevent and treat eubacterial diseases.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of microorganisms, in particular to penicillium LDFST10 and application of the penicillium LDFST10 in promoting plant growth or preventing and treating stem rot. The preservation number of the penicillium LDFST10 is CGMCC (China General Microbiological Culture Collection Center) No. 41960. The application comprises the step of applying the penicillium LDFST10 or the fermentation product thereof to rhizosphere soil of the plant so as to promote the growth of the plant and prevent and treat basal stem rot. The invention researches and screens out the penicillium frutescens LDFST10 capable of promoting plant growth and inhibiting various pathogenic bacteria of stem rot, various growth promoting preparations or bacteriostatic agents or drugs aiming at the stem rot can be developed on the basis of the penicillium frutescens, and the penicillium frutescens LDFST10 has important value in the planting field of various crops such as corn, wheat and the like.

The invention relates to a preparation method and application of antibacterial and starch aging resistant biphase gel, and belongs to the field of food processing. The preparation method is characterized by comprising the following steps: preparing oleogel from monoglyceride, corn oil and curcumin, preparing hydrogel from wheat bran araboxylan and tea polyphenol, mixing the oleogel and the hydrogel, adding lecithin, and homogenizing at a high speed to obtain the gel with a two-phase system. The biphase gel produced by the invention has the characteristics of zero trans-fat, high fiber content, simple preparation method and the like, realizes synergistic stability of oil and water phases, has both the mechanical strength of oil gel and the biocompatibility of hydrogel, can replace shortening in baked food, and has a wide application prospect. And the shelf life of the baked product is prolonged through the synergistic effect of antibiosis and starch aging resistance. The mass ratio of the oil phase to the water phase in the biphase gel is 1: 3-3: 1, the addition amount is 10-20% of the mass of the flour, the bacteriostasis rates on penicillium and rhizopus are 60-85% and 65-90% respectively, the starch crystallinity in the bread storage process is reduced by 10-20%, and the shelf life of the bread is prolonged by 10-20 days.

The invention belongs to the field of microorganisms, and particularly provides a non-pathogenic penicillium ochrochloron strain which is derived from garlic rhizosphere soil, is classified and identified as penicillium ochrochloron, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC 41151. The strain has broad-spectrum bacteriostatic activity and can antagonize various plant pathogens; the plant growth promoting agent has the growth promoting characteristic, can secrete siderophores and phospholytic enzymes, and improves plant iron and phosphorus nutrition; the composition is safe and free of pathogenicity to crops. Tests show that the P4 strain can relieve plant iron deficiency yellowing and promote crop growth. The invention also provides application of the strain in biological control of plant diseases and plant growth promotion.

The present invention generally relates to an isolated Penicillium strain, named Penicillium olsonii TLL1, with a preservation number of NRRL 68252, and to the use thereof in promoting plant growth, and to the use of the Penicillium strain, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1. Specifically, the present invention provides compositions suitable for promoting plant growth comprising the isolated fungal strains that can mitigate Al toxicity 5 and increase phosphate effectiveness, as well as methods of their promotion of plant growth.

The invention relates to a preparation method of a plant disease control fungicide, which comprises the following steps: taking traditional Chinese medicines with an antibacterial effect as raw materials, and carrying out first-step fermentation by adopting Trichoderma harzianum; beauveria bassiana and Penicillium baylii are subjected to second-step fermentation, bacillus velezensis is subjected to third-step fermentation, fermentation liquor is collected, a filter cake is further subjected to reflux extraction, an extracting solution and the fermentation liquor are collected, combined and concentrated into clear paste, an ethanol solution is added, standing is carried out, supernate is taken, borneol, gelatin and cellulose acetate are added to prepare mixed liquor, ultrafiltration is carried out, ultrafiltrate is collected, and freeze-dried powder is obtained. The microbial inoculum required by the invention is obtained. According to the invention, specific traditional Chinese medicines are adopted, an excellent broad-spectrum antibacterial system is formed through specific microbial fermentation, and the antibacterial system is compounded with borneol, gelatin, cellulose acetate and other components to form the fungicide, so that the effectiveness duration and the effect taking rate of the fungicide on the surface of a plant are effectively improved, and efficient prevention and treatment on various diseases such as fungi, bacteria and the like are formed.

The invention relates to the technical field of agricultural microorganisms, and discloses a complex microbial inoculant for improving soil micro-ecology and promoting plant growth and application thereof.The complex microbial inoculant is prepared from 30-40 parts of bacillus subtilis, 20-40 parts of pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of trichoderma and 5-10 parts of penicillium; wherein the bacillus subtilis is bacillus subtilis L11 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.31274, the preservation date is July 11, 2024, and the bacillus subtilis is classified and named as bacillus subtilis. The complex microbial inoculant for improving the soil micro-ecology and promoting the plant growth is adopted, a multi-bacterium synergistic community is constructed, the complementary metabolism advantage of different strains at the rhizosphere is utilized, the nutrient conversion and stress resistance characteristics are improved, and therefore the soil micro-ecology is improved, and the plant root development is promoted.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The invention discloses a method for converting phosphorus in bone black into soluble phosphorus by adopting penicillium desert, and belongs to the technical field of waste conversion and utilization. According to the method, the indissolvable phosphorus in the bone black is efficiently and stably converted into the soluble phosphorus by utilizing the specifically preserved Penicillium desertorum, the phosphorus dissolving amount within 10 days is up to 2187.50 mg / L, the process is environment-friendly, the raw material adaptability is high, and a feasible microbial conversion way is provided for high-valued utilization of bone black phosphorus resources.

The invention belongs to the field of strains, and particularly relates to a botryosphaeria dothidea attenuated strain ZMXX551-1 containing a penicillium-producing virus BdCV2 and application of the botryosphaeria dothidea attenuated strain. The attenuated strain ZMXX551-1 is preserved in the China General Microbiological Culture Collection Center on June 25, 2025, the preservation number is CGMCC NO.42040, the genome of the penicillium virus BdCV2 is composed of four dsRNAs, the penicillium virus BdCV2 has a remarkable effect in the aspect of weakening the pathogenicity of botryosphaeria dothidea, the attenuated strain ZMXX551-1 is used for treating peach seedlings, the length of scabs is inhibited by 8.63%, and the length of the scabs is inhibited by 8.63%. After 3 months, the scab of the treatment group begins to heal, and the scab of the control group is continuously increased, which indicates that the peach seedling inoculated with the strain ZMXX551-1 grows well; therefore, the strain ZMXX551-1 can inhibit expansion of peach gummosis scabs and improve plant growth, and has biological control potential.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The embodiment of the invention discloses yarrowia caribeckii as well as a separation and screening method, an identification method and application of the yarrowia caribeckii. The preservation number of the yarrowia caribica is CGMCC (China General Microbiological Culture Collection Center) No.27604, and the preservation number of the yarrowia caribica is CGMCC No.27604. The Caribik Yarrowia spp. Provided by the embodiment of the invention has a good antagonistic effect on gray mold, penicilliosis, black spot and aspergillus niger after fruits are picked, and can obviously reduce the disease morbidity of the fruits of strawberries, apples, tomatoes and pears. And the antagonistic bacterium is identified to be Meyerozyma caribbica, and the antagonistic bacterium can be used as the Meyerozyma caribbica. By respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris, the disease prevention and control effects of Meyerozyma caribbica can be enhanced. The disease prevention and control effects of the Meyerozyma caribbica can be enhanced by adding the auxiliary factors of calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The invention relates to a method for preparing a Ginkgo biloba leaf extract highly concentrated in proanthocyanidins, and practically free of flavonoids and terpene trilactones. The invention also relates to the use of cheese fungal strains such as Penicillium Roquefortii PA and Geotrichum Candidum Geo 15, to catabolize high molecular weight proanthocyanidin polymers into small molecules in order to have beneficial effects on health.

The invention provides a genetic engineering strain of high-yield immunosuppressant mycophenolic acid as well as a preparation method and application of the genetic engineering strain. The invention firstly provides a method for constructing the penicillium with the effect of high yield of mycophenolic acid. The method comprises the step of replacing an indigenous promoter of acyl-coenzyme A oxidase PbACOX323 protein participating in beta oxidation in peroxisome of a penicillium strain with a promoter PgpdA of 3-phosphoglycerol dehydrogenase with an amino acid sequence of SEQ ID NO: 1. According to the method disclosed by the invention, the genetically engineered bacterium with high yield of mycophenolic acid is constructed, the yield of mycophenolic acid is positively increased by replacing a promoter of acyl-coenzyme A oxidase PbACOX323, and the method has a good industrial application value.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

The present application belongs to the technical field of postharvest disease control, and particularly relates to a biocontrol Serratia marcescens and its use for postharvest disease control and storage of pears. The steps are as follows: the Serratia marcescens is activated and cultured, and the bacterial cells are obtained by centrifugation after the culture; the bacterial cells are resuspended in sterile physiological saline to obtain a bacterial suspension; the bacterial suspension is injected into the holes punched on the surface of the pears, and then an equal volume of Penicillium expansum spore suspension is injected, so that the effective control of the Penicillium disease caused by Penicillium expansum is achieved; or the bacterial suspension of the Serratia marcescens is uniformly sprayed on the surface of the pears, and then naturally dried, so that the use for postharvest disease control and storage of the pears is achieved; the present application can significantly reduce the occurrence of the postharvest Penicillium disease of the pears, and reduce the natural rot rate of the pears after harvest, and is safe and environmentally friendly, and not only has no significant adverse effect on the main quality indicators of the postharvest pears, but also can maintain the storage quality of the pears, and achieves a significant technical effect, and has a wide application prospect.

The invention belongs to the technical field of microorganisms, and discloses an amorphophallus bulbifer southern blight infection influence analysis method based on metabonomics and microbiomics. Conjoint analysis shows that core florae (such as Streptomyces, Penicillium and Bauldia) are in significant positive correlation with phenylpropanoids and glutathione (succinic acid, coniferyl alcohol and glutamic acid), succinic acid is in positive correlation with Kribbella and Terrimonas, 2 ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', 2' ', the 3, 3 '-cycle CMP is negatively correlated with pathogen-related bacteria, which indicates that the metabolite regulates and controls the abundance of specific bacteria through positive / negative feedback so as to drive the microecological balance of rhizosphere, and a collaborative defense strategy that plants recruit beneficial microorganisms and inhibit pathogenic bacteria through metabolism reprogramming is disclosed.

This invention discloses a compound fungal agent containing Penicillium citrinum and Trichoderma guizhouense, wherein the Penicillium citrinum is Penicillium citrinum (… Penicillium citrinum NJAU-T106, the Guizhou Trichoderma is Guizhou Trichoderma ( Trichoderma guizhouense NJAU4742. This invention also discloses its preparation method and its application in promoting banana plant growth, increasing banana yield, and controlling banana wilt disease. This invention screened Penicillium citrinum (NJAU4742) from banana disease-suppressing soil to obtain a strain with good biocontrol and growth-promoting effects. Penicillium citrinum The study used NJAU-T106 and innovatively combined it with Trichoderma guiyuanensis NJAU4742 to construct a synergistic system of Penicillium citrinum and Trichoderma guiyuanensis. This effectively reduced the incidence of banana wilt disease while better promoting banana plant growth and increasing banana yield, thereby driving the green and sustainable development of the banana industry.