51 results about "Biogenic amine" patented technology

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

The invention relates to a pet food and a processing method thereof, and relates to the technical field of pet food processing, the pet food comprises the following components in parts by mass: meat meal, prebiotics, animal fat, a mineral additive, a vitamin additive, an edible binder, and a biogenic amine complexation-decarboxylase inhibitor; the processing method comprises the steps of raw material mixing and forming. According to the pet food and the processing method thereof, on the premise that basic nutrition needed by growth and development of pets is met, the effects of blocking generation of biogenic amine, complexing generated biogenic amine and reducing the content of volatile basic nitrogen are achieved, meanwhile, the palatability and storage stability of the pet food are improved, and the pet food is suitable for popularization and application. And the health risks of intestinal discomfort and the like caused by ingestion of biogenic amine by pets are reduced.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention discloses an SERS (Surface Enhanced Raman Scattering) aptamer sensor based on a double-layer core-satellite magnetic gold nanostructure and a method for detecting biogenic amine in food by using the SERS aptamer sensor. The Fe3O4 (at) Au MNPs nanoparticles are combined with a nucleic acid aptamer, and a specific magnetic capture probe is obtained; the method comprises the following steps: mixing gold nanoparticle solutions with different particle sizes with 4-MBN, incubating to obtain a'biological silence zone 'SERS tag, and combining the'biological silence zone' SERS tag with pretreated sulfydryl modified c-DNA to obtain a specific SERS signal probe; and oscillating and incubating the specific SERS signal probe solution and the specific magnetic capture probe solution to obtain the SERS aptamer sensor based on the double-layer core-satellite magnetic gold nanostructure. According to the method, a'biological silence zone 'SERS label is combined with a magnetic induction technology, so that the problems of poor anti-interference performance and poor accuracy during detection of trace components in a complex matrix by SERS are solved, and high-efficiency detection of tryptamine in biogenic amine is realized.

The invention belongs to the technical field of wine brewing, relates to a natural sweet wine low-sulfur brewing method for delaying white grapes until the white grapes turn red and then harvesting the white grapes, solves the health hidden danger and flavor defects caused by high-sulfur addition in the traditional sweet white wine process, and remarkably improves the flavor quality of the wine at the same time. According to the method, complete and healthy red (total anthocyanin content is greater than or equal to 5mg / kg) fruits are selected through artificial spike selection and grain selection, and the cardinal number of infectious microbes in raw materials is reduced; no commercial yeast is added, natural slow fermentation is achieved by means of microorganisms, and the finished product is good in flavor and quality; gallic acid, condensed tannin and hydrolyzed tannin with a ratio of 1: 1: 1 are compounded to prepare wine-brewing tannin, and the wine-brewing tannin and low-dose sulfur dioxide construct a synergistic system, so that the sulfur consumption of sulfur dioxide is greatly reduced, secondary fermentation of high-sugar wine liquid is prevented, and infectious microbes and wine body oxidation are effectively inhibited. The finished product biogenic amine, pesticide and heavy metal residues all meet the safety standard, mycotoxin is not detected, meanwhile, the original flavor of the wine body is prominent, the storage stability is good, and dual guarantee of health and quality is achieved.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The invention relates to the technical field of wine brewing, in particular to hansenula polymorpha cloud-268, application of the hansenula polymorpha cloud, a fermentation method of dry red wine and a microbial agent. The hansenula polymorpha var. Uvarum cloud-268 is preserved in the China Center for Type Culture Collection on December 13, 2013, and the preservation number of the hansenula polymorpha var. Uvarum cloud-268 is CCTCC NO: M2013658. According to the invention, non-saccharomyces cerevisiae YUN-268 and saccharomyces cerevisiae are preferably selected for synergistic fermentation, and the stability of a fermentation system is maintained by using the fermentation biological protection effect of the non-saccharomyces cerevisiae. Accumulation of potential harmful organism amine is remarkably reduced, generation of aroma substances of the grape wine is promoted, and the aroma of the obtained grape wine is richer. The method provides a feasible and efficient technical scheme for green brewing of low-sulfur and high-quality wine, has the advantages of greenness, safety and high quality, and provides a technical path capable of being popularized for industrial production of the low-sulfur dry red wine.

The application discloses a non-membrane-producing Rhodotorula conjugata capable of inhibiting membrane-producing microorganisms, a microbial inoculum and application, relates to the technical field of microorganisms and food fermentation, and the Rhodotorula conjugata (KDX-69) is preserved in the Guangdong Microbial Culture Collection Center on Nov. 14, 2025, and has a preservation number of GDMCC No: 67296. Zygosaccharomyces rouxii The new strain is obtained from a bean paste, does not produce membranes by itself, and can inhibit the growth of membrane-producing microorganisms; the new strain is applied to a low-salt fresh pepper bean paste fermentation process, can effectively inhibit membrane production, flower production and other spoilage and deterioration, reduce the content of biological amine in a product, improve product safety, improve product flavor, and has high application value.

The application discloses a kind of meat preservation and freshness visual double-effect nano intelligent indicating film and preparation method thereof, belong to food packaging storage and detection technical field.The preparation method of the intelligent indicating film includes the following steps: (1) poly-3-hydroxybutyric acid-co-oxyvalerate (PHBV) and ferulic acid are dissolved in chloroform solution;(2) polyvinyl alcohol (PVA), konjac glucomannan (KGM), alizarin (AL) are mixed, and polyvinyl alcohol-konjac glucomannan-alizarin mixed solution is obtained by removing bubble;(3) PH-F solution is dried by electrospinning technology, and PVA-KGM-AL@PH-F double-layer indicating film is obtained.The application prepares meat freshness indicating film with efficient antibacterial performance and high sensitivity color response performance by regulating the pH of film-forming solution.In the intelligent indicating film, alizarin responds to meat spoilage biogenic amine, and the color of indicating film gradually changes from cream (slightly yellow) to light pink or even deep purple;By comparing the intelligent indicating film after reflection with colorimetric card, the freshness of meat can be judged.

This invention provides a rare earth metal-based ternary composite antibacterial agent and its preparation method. The antibacterial agent is prepared from raw materials including GL-22, Eu-containing polyoxometalate clusters, and Spm. The rare earth metal-based ternary composite antibacterial agent of this invention, after fractional compounding of Eu-containing polyoxometalate clusters with peptides and biogenic amines, exhibits highly efficient antibacterial function against Escherichia coli and Staphylococcus aureus. The ternary assembly EuW... 10 The fluorescence emission of / GL-22 / Spm is enhanced by 30-40 times, and it is effective against Escherichia coli (E. coli). E. coli The antibacterial properties of the drug have been increased from 40%~30% to 90%~95%, and its efficacy against bacteria has been improved. E. coli The inhibition rate of biofilm (BME) formation was 21.8%.

This invention discloses a method for preparing a highly antioxidant, color-changing, intelligent active food preservation film containing bilberry anthocyanins, belonging to the field of food preservation technology. An ACNs-CHC / CMC-WPI nanocomposite is constructed through the encapsulation of chitosan hydrochloride (CHC), carboxymethyl chitosan (CMC), and whey protein isolate (WPI). Using corn starch, chitosan, and 30 wt% glycerol as a base material, the anthocyanin-loaded nanocomposite is introduced as a functional component and formed on the surface of an acrylic sheet via solution casting. This food preservation film exhibits excellent mechanical properties and effectively inhibits spoilage and the proliferation of pathogenic bacteria in high-protein aquatic foods (such as white shrimp), reduces the formation rate of biogenic amines, and significantly slows down the deterioration process of food quality. Furthermore, the surface color of the film changes with the pH value of the target meat, providing a visual monitoring method for food freshness and can also be used for rapid detection of food freshness.

The invention discloses a camellia chicken source bacillus velezensis CH3-2 and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis CH3-2 obtained through separation shows high tolerance to acid and cholate, has excellent adhesion capacity, and shows broad-spectrum antibacterial and excellent antioxidant activity. Safety evaluation proves that the strain CH3-2 has no hemolytic activity, does not generate biogenic amine and does not contain virulence genes. Six antibiotic resistance genes which are inherent and coded by chromosomes and carried by the gene have no risk of horizontal transfer. A mouse acute oral toxicity test further verifies the in-vivo safety of the strain, the strain has no adverse effect on Kunming mouse tissues, and an excellent strain resource is provided for developing functional probiotic preparations or feed additives for poultry.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

The application discloses a kind of meat freshness rapid test paper, belong to food rapid detection technical field.The test paper is with water absorption filter paper as base material, and is loaded by the reaction color developing system consisting of o-Phthaldehyde, mercapto glycolic acid, sodium acetate buffer and fast blue B salt, can occur specific color reaction with the biological amine (putrescine) generated in the meat spoilage process, and realizes freshness rapid judgment by color ladder change.The detection time of the application is only 3-5 minutes, without large instrument, simple operation, high sensitivity, low cost, wide spectrum, can be used for on-site rapid screening of pork, beef, mutton, frozen shrimp, frozen chicken breast and other various meats, and is widely applicable to raw material acceptance of meat processing enterprises, market supervision law enforcement and cold chain logistics quality monitoring.

The invention belongs to the technical field of feed processing, and particularly provides a preparation method of a bacterium-enzyme synergistic fermentation liquid feed, which comprises the following steps: adding water into feed raw materials, mixing, and adding a compound enzyme preparation for enzymolysis; the compound enzyme preparation comprises alkaline protease and xylanase; cooling the material liquid after enzymolysis, and inoculating a complex microbial inoculant for fermentation; the complex microbial inoculants comprise lactobacillus plantarum, enterococcus faecium and KM71 pichia pastoris; and after the fermentation is finished, sterilizing to obtain the bacterium-enzyme synergistic fermentation liquid feed. According to the preparation method provided by the invention, the pre-enzymolysis of macromolecular substances of the feed and the fermentation effect of microbial flora are cooperated, so that the content of nutrient substances in the feed is remarkably increased. Through sequential addition of an enzyme preparation and a microbial agent, two-stage precise temperature control fermentation and in-situ low-temperature pasteurization technology, fermentation is terminated on the premise of not destroying nutrition, and the problems of nutrition improvement, biogenic amine control and product shelf life are solved.

The invention discloses a salt-tolerant Bacillus velezensis strain capable of efficiently degrading protein and biogenic amine and an application of the salt-tolerant Bacillus velezensis strain. The bacillus velezensis is a strain FJNU-SIO1, is classified and named as bacillus velezensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is September 26, 2025, the preservation number is CGMCC No.36086, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The strain FJNU-SIO1 is wide in growth adaptation range, can tolerate the salt concentration of 1%-10%, and can still efficiently degrade protein and nine common biogenic amines including putrescine, cadaverine, histamine and tyramine under the high salt concentration of 10%, the degradation rate of the protein can reach 47.68%, and the degradation rate of the total biogenic amines can reach 32.77%. The strain FJNU-SIO1 provided by the invention has the outstanding advantages of simplicity and convenience in operation, low cost, wide applicability and the like when being applied to degradation treatment of proteins and biogenic amines, and has an important value for guaranteeing safe production of fermented foods.

The invention belongs to the field of food packaging and food detection, and particularly relates to a method for preparing an amine response type nanofiber indicating film through electrostatic spinning and application of the amine response type nanofiber indicating film to food freshness detection. The method comprises the following steps that polycaprolactone serves as a film forming base material, polydiacetylene serves as an indicator, polyacrylic acid serves as an auxiliary agent, and the amine response type nanofiber indicating film is prepared through electrostatic spinning; the preparation method comprises the following steps: preparing a nanofiber membrane by adopting an electrostatic spinning technology, and preparing an amine response type nanofiber indication membrane through irradiation of an ultraviolet lamp; the prepared amine response type nanofiber indicating film has good biological safety and mechanical performance, the indicating film added with polydiacetylene and polyacrylic acid can generate obvious color change on low-concentration biogenic amine, and the freshness change condition of meat food such as shrimps in the transportation and storage period can be effectively indicated. The intelligent food freshness indicating film prepared by the method has wide application value in the field of intelligent food packaging.

The application discloses a compound Cl-BODIPY used as a fluorescent probe for rapid nondestructive detection of food freshness, and also establishes a corresponding preparation method and detection method. The probe produces a color and fluorescent dual optical signal change through specific interaction of a recognition group with volatile biogenic amines, and provides a simple and convenient detection method for rapid detection of volatile biogenic amines. The fluorescent probe is simple to prepare, low in cost, and has the characteristics of high sensitivity, high selectivity and rapid response, can in-situ rapidly detect volatile biogenic amines produced by fish and meat food spoilage, and judges the freshness (biogenic amine content) of fish and meat through colorimetric and fluorescent dual visual signal changes, thereby providing an effective means for rapid nondestructive detection of food freshness, having great application prospect and great popularization significance.

Disease modification of specific neurodevelopmental disorders is demonstrated for preschool children through modulation of specific biogenic amine neurotransmitters and y-aminobutyric acid (GABA) / glutamine. In specific, cumulative reductions in maladaptive behaviors that interfere with activities of daily living for preschool populations are achieved through staged, long term, treatment. Additionally, a prophylaxis for certain neurodegenerative disorders is presented. Provided herein are compositions comprising digestive enzyme preparations and methods of using same for treating an Autism Spectrum Disorder (ASD).

The invention discloses a method for reducing the content of biogenic amines in meat products through composite vacuum rapid pickling, and belongs to the technical field of food processing. According to the method disclosed by the invention, the pork tenderloin, the chicken breast and the beef tendon are pickled by adopting the pickling liquid prepared by compounding the anthocyanin and the lipopeptide and assisting ultrasonic and vacuum modes, so that the pickling time of the meat product is shortened, the quality of the meat product is improved, the generation of biogenic amine is also inhibited, histamine and tyramine are not detected in the pork tenderloin pickled by the method, and the pork tenderloin is free of toxic and side effects. The content of cadaverine and spermidine is remarkably reduced, and the product is uniform in meat quality, excellent in flavor and high in safety. The method provides a theoretical basis for controlling the safety of meat products and optimizing the processing technology while improving the meat pickling efficiency, and has a key guiding significance for developing low-salt and low-biogenic-amine meat products.

The present disclosure relates to a formulation for iron chelation therapy comprising: a water-soluble polyphenol-biogenic amine complex comprising of a polyphenol and an amine, and a 0.9% of sodium chloride; optionally one or more additive, wherein water-soluble polyphenol-biogenic amine complex effectively chelates the excess iron and enable its elimination. The present disclosure also provides a method of preparation of a formulation for iron chelation therapy comprising: a) adding 0.005 to 5% w / w of a polyphenol and 0.005 to 5% w / w of an amine followed by adjusting to pH-7 by adding HCl / NaHCO3 to form a mixture; and b) mixing the mixture of step a) with suitable proportion in 0.9% sodium chloride and optionally one or more additive to form a formulation for iron chelation therapy, wherein water-soluble polyphenol-biogenic amine complex effectively chelates the excess iron and enable its elimination.

The invention discloses an application of a reagent containing multi-copper oxidase in reducing the biogenic amine content of a fermented meat product, recombinant multi-copper oxidase is obtained through a genetic engineering technology, and the multi-copper oxidase is developed into an enzyme reagent for degrading biogenic amine in Sichuan sausages. Results show that the addition of the reagent containing the multi-copper oxidase and the diamine oxidase has no influence on the eating quality and the sensory quality of the Sichuan sausages, in terms of the biogenic amine content, after the reagent is added, the degradation rates of histamine, tyramine, cadaverine and putrescine are at least 49.63%, 38.51%, 38.26% and 35.40%, and the degradation rates of the reagent containing the multi-copper oxidase and the diamine oxidase are at least 49.63%, 38.51%, 38.26% and 35.40%. The reagent provided by the invention has a good degradation effect on four main biogenic amines in Sichuan sausages.

The present application belongs to the technical field of high polymer materials, and relates to a multifunctional cellulose-based fluorescent material as well as a preparation method and application thereof. The cellulose-based fluorescent material has a molecular structure shown in the following formula I: wherein a molecular main chain is cellulose, R2 is H or formula II, and x is an integer of 0-2. The molecular main chain of the cellulose-based fluorescent material is cellulose, and the cellulose skeleton contains three types of substituent groups. The first type is a cellulose ester or cellulose ether with R1 structure; the second type is a cellulose carbonate, which endows the cellulose-based fluorescent material with a Fe 3+ fluorescent response performance; and the third type is H on a coumarin or a substituent group as shown in formula II, which can endow the cellulose-based fluorescent material with a response performance and other special performances. Therefore, the multifunctional cellulose-based fluorescent material can simultaneously realize detection of two different target substances, i.e., Fe 3+ and alkaline substances such as biological amines.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.

The invention discloses a non-film-producing zygosaccharomyces rouxii strain capable of inhibiting film-producing microorganisms, a microbial agent and application, and relates to the technical field of microorganisms and food fermentation, Zygosaccharomyces rouxii KDX-69 is preserved in the Guangdong Microbial Culture Collection Center on November 14, 2025, the preservation date is November 14, 2025, and the preservation number is GDMCC No: 67296. The invention further discloses a preparation method of the non-film-producing zygosaccharomyces rouxii strain capable of inhibiting the film-producing microorganisms, a microbial agent and application of the non-film-producing zygosaccharomyces rouxii strain capable of inhibiting the film-producing microorganisms, the microbial agent and the application of the non-film-producing zygosaccharomyces rouxii strain. According to the invention, a new strain which does not produce a film and can inhibit the growth of film-producing microorganisms is screened from the thick broad-bean sauce; when the new strain is applied to the fermentation process of low-salt fresh pepper bean halves, spoilage and deterioration such as film production and flower production can be effectively inhibited, the content of biogenic amine in the product is reduced, the safety of the product is improved, the flavor of the product is improved, and the application value is very high.

The invention relates to a fermentation method for reducing biogenic amine in fish sauce, which comprises the following steps: 1) taking fresh fish meat, adding 30-50% of sterile water, 18% of salt, 0.5-2% of neutral protease with activity of 2 * 10 < 4 > U / g and 0.03% of sodium pyrophosphate by mass, uniformly mixing and crushing, and bottling; (2) fermenting at the constant temperature of 35 DEG C for 72 days, determining the oxidation-reduction potential in the fermentation process of the fish sauce by using an oxidation-reduction potential depolarization method automatic determinator in the fermentation process, adding a proper amount of hydrogen peroxide solution with the mass fraction of 0.5% every three days, controlling the oxidation-reduction potential of the fish sauce fermentation liquid to be 300-340mV until the fermentation end point, and during the fermentation period, adding a proper amount of water into the fish sauce fermentation liquid, water is supplemented once every three days. The fermentation method is simple to operate and easy to implement, the content of biogenic amine, especially tyramine, of the fish sauce is remarkably reduced, and the quality of the obtained fish sauce is good.

The invention provides a tissue culture and rapid propagation method suitable for ornamental taro, and relates to the technical field of plant tissue culture. Comprising the following steps: step 1, preparing materials, namely selecting healthy ornamental taro plants without diseases and insect pests, taking tender lateral buds, stem tips or leaves of the ornamental taro plants as explants, cleaning the explants, disinfecting and cutting the explants; step 2, induction culture: taking MS as a basic culture medium, adding biogenic amine, NAA, TDZ, coconut milk, cane sugar and ascorbic acid to prepare an induction culture medium, and then culturing the explant obtained in the step 1 in a constant-temperature and light-dark culture environment until visible green buds germinate. Through ultrasonic-assisted combined disinfection of the explant, the pollution rate is obviously reduced and the induction culture effect is improved on the premise of reducing the damage of the explant, and through the induction culture of a modulated light source and on the basis of inhibiting spindling, the bud is more robust, the chlorophyll content is increased, and the bud can be continuously subcultured for 8-10 times after multiplication culture without obvious degeneration.

The application discloses a kind of biogenic amine response fluorescent color-changing hydrogel microneedle patch, and preparation method and application thereof, belong to food detection technical field.The method for preparing biogenic amine response fluorescent color-changing microneedle patch of the application includes the following steps: (1) two fluorescent dyes are dissolved in dimethyl sulfoxide and ultrapure water respectively, after mixing according to proportion, obtain solution A that responds to biogenic amine;(2) solution A is added to hydrogel material, horseradish peroxidase (HRP), hydrogen peroxide (H2O2) to prepare mixed fluorescent hydrogel solution, which is dropped on the mold containing patch needle tip prepared;By drying, fluorescent color-changing hydrogel microneedle patch is prepared.The microneedle patch prepared by the method of the application is applied to rapid biogenic amine response, can respond to color in the storage process of fresh meat produced by biogenic amine, suitable for consumer on-site detection of fresh meat, so as to purchase healthy, high-quality food, with wide application prospect.

The invention provides a fluorescence detection method for detecting histamine, hydrogel and a preparation method and application thereof, and belongs to the technical field of biogenic amine detection. In particular to a fluorescence detection method for detecting histamine, which mainly comprises the following steps: mixing a copper nano-cluster solution, a carbon dot solution and histamine solutions with different concentrations, then fixing the volume, detecting the fluorescence emission spectrum of each system under the excitation wavelength of 365 nm, and calculating the fluorescence intensity of each system by taking the ratio F437 / F605 of the fluorescence intensity at the wavelength of 437 nm and 605 nm as the ordinate. Making a standard curve by taking the concentration of histamine in each system as an abscissa, and detecting the concentration of histamine in a sample to be detected; the invention also provides a hydrogel which is used for detecting histamine. The fluorescence detection method provided by the invention is used for detecting histamine, the histamine detection selectivity is strong, the specificity is good, and the hydrogel prepared by the invention realizes visual rapid detection of histamine.

The application provides a fluorescent probe for biological amine ratio type detection and a preparation method and application thereof, and belongs to the technical field of fluorescence sensing. The A-D-A type organic fluorescent small molecule material of the application adopts a conjugated structure containing a hydrogen bond binding site as a donor, and can form an intramolecular charge transfer (ICT) state after introducing an electron-withdrawing group. The purpose of fluorescence detection of biological amine is achieved by the change of ICT properties before and after the hydrogen bond action. The fluorescence detection of biological amine by the material has the advantages of high sensitivity, rapid response, significant color change and reusability. Further, the application also constructs a portable detection platform based on a smart phone. Through digital analysis of the fluorescence image, the visual and quantitative detection of food freshness is realized.