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127 results about "Biogenic amine" patented technology

A biogenic amine is a biogenic substance with one or more amine groups. They are basic nitrogenous compounds formed mainly by decarboxylation of amino acids or by amination and transamination of aldehydes and ketones. Biogenic amines are organic bases with low molecular weight and are synthesized by microbial, vegetable and animal metabolisms. In food and beverages they are formed by the enzymes of raw material or are generated by microbial decarboxylation of amino acids.

Polyimidazolone compound as well as preparation method and application thereof

The invention discloses a polyimidazolone compound as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a bilateral alkyne ester compound, amidine hydrochloride, a catalyst and a desalting agent in an organic solvent, and carrying out polymerization reaction to obtain the polyimidazolone compound. The polyimidazolone compound with the five-membered ring is selectively generated, and the method has the advantages that monomers are easy to obtain, operation is easy, and by-products are low in toxicity. The polyimidazolone compound prepared by the invention has better machinability and has potential application value in the aspects of metal ion detection and biogenic amine detection.
Owner:SOUTH CHINA UNIV OF TECH

Fluorescent material based on dicyanoethylene as well as preparation method and application of fluorescent material

The invention relates to the technical field of biogenic amine detection, in particular to a dicyanoethylene-based fluorescent material as well as a preparation method and application thereof. After the biogenic amine is in contact with the glass surface fluorescent probe, due to the nucleophilic characteristic of the biogenic amine, the biogenic amine can be subjected to nucleophilic substitution reaction with a probe molecule dicyanovinyl group to generate an imine chemical structure. As the electron-withdrawing capability of dicyanoethylene is stronger than that of imine, the intramolecular charge transfer effect of probe molecules is greatly reduced, so that blue shift of absorption and luminescence properties of the probe molecules is caused, and after the fluorescent probe is loaded on the surface of glass, the fluorescence intensity of the fluorescent probe is improved. The formed dicyanoethylene-based fluorescent material provides a solution for the technical problem that the existing biogenic amine detection mode is inconvenient.
Owner:HUAINAN NORMAL UNIV +1

Chickpea composite tempeh and preparation method thereof

Chickpea with peel is used as a main raw material, black rice and quinoa are compounded, pretreatment is performed through an ultrasonic-assisted enzyme method, efficient softening is achieved, and a three-stage mixed fermentation process is adopted: in the first stage, lactic acid bacteria are utilized for pre-fermentation for acidification, infectious microbes are inhibited, and generation of biogenic amine is controlled; in the second stage, carrying out gradient temperature fermentation by adopting the danbei fungus powder, firstly promoting mycelial growth at low temperature, and then exciting a Maillard reaction at high temperature to improve the flavor; in the third stage, probiotic microcapsules are added for after-ripening, biogenic amine is further degraded, the flavor is improved, the product is endowed with probiotic activity, and a bacteriostatic protective layer is provided. The method successfully solves the problem that the texture is rough due to the fact that the chickpea skin is reserved, and the final product has the comprehensive advantages of being rich in nutrition, good in taste, high in safety and strong in probiotic function and is high in sensory acceptability.
Owner:TARIM UNIV

Porphyrin metal organic framework composite material, preparation method and biogenic amine SERS (Surface Enhanced Raman Scattering) array sensor

The invention relates to a porphyrin metal organic framework composite material, a preparation method and a biogenic amine SERS (Surface Enhanced Raman Scattering) array sensor. The preparation method of the composite material comprises the steps that a copper precursor solution is added into a surfactant, heating reaction is conducted, a reducing agent is added, heating reaction is conducted, and copper nanoparticles are obtained; the preparation method comprises the following steps: adding a transition metal salt and a surfactant into an organic solvent, adding a porphyrin ligand, carrying out a solvothermal reaction, carrying out centrifugal separation, washing and drying to obtain a porphyrin metal organic framework material, and dispersing the porphyrin metal organic framework material into the organic solvent to obtain a dispersion liquid A; and dispersing copper nanoparticles in the dispersion liquid A, carrying out a second heating reaction, and carrying out centrifugal separation, washing and drying to obtain the porphyrin metal organic framework composite material. The invention also provides the porphyrin metal organic framework composite material prepared by the preparation method, and the application of the porphyrin metal organic framework composite material as a biogenic amine SERS array sensor. The invention solves the problem that the accuracy of the detection result is influenced because the traditional biogenic amine detection method is difficult to realize field detection.
Owner:CHONGQING TECH & BUSINESS UNIV

Degradation of harmful biogenic amines in food with a new microbial enzyme derived from glutamicibacter halophytocola

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.
Owner:UNIV HOHENHEIM

Chicken freshness detection method and system combining colorimetric gel label and convolutional neural network

The invention discloses a chicken freshness detection method and system combining a colorimetric gel label and a convolutional neural network, and belongs to the technical field of food safety detection. An indicating gel dot matrix label is prepared from curcumin, anthocyanin and betacyanin, the optimal load concentration of curcumin, anthocyanin and betacyanin is optimized and determined, and sensitive color response is generated to volatile biogenic amine gas such as dimethylamine and trimethylamine; gel label images are collected, the images are divided into fresh samples, secondary fresh samples and decayed samples in combination with the TVB-N content, the samples are subjected to data enhancement and then input into an OfficientNet-B0 model for training, and efficient classification is achieved. According to the method, a detection system combining the natural pigment-based multi-channel colorimetric gel and the lightweight convolutional neural network is constructed, lossless, rapid and intelligent detection of the freshness of the fresh chicken is realized, and a green and efficient solution is provided for real-time monitoring of the freshness of food and application of a mobile terminal.
Owner:BOHAI UNIV

Pet food and processing method thereof

The invention relates to a pet food and a processing method thereof, and relates to the technical field of pet food processing, the pet food comprises the following components in parts by mass: meat meal, prebiotics, animal fat, a mineral additive, a vitamin additive, an edible binder, and a biogenic amine complexation-decarboxylase inhibitor; the processing method comprises the steps of raw material mixing and forming. According to the pet food and the processing method thereof, on the premise that basic nutrition needed by growth and development of pets is met, the effects of blocking generation of biogenic amine, complexing generated biogenic amine and reducing the content of volatile basic nitrogen are achieved, meanwhile, the palatability and storage stability of the pet food are improved, and the pet food is suitable for popularization and application. And the health risks of intestinal discomfort and the like caused by ingestion of biogenic amine by pets are reduced.
Owner:WILPTON (SHANDONG) PET FOOD CO LTD

Lactobacillus paracasei CN23, preparation method of microbial inoculum and preparation method of fermented bean curd

The invention discloses a lactobacillus paracasei CN23, a preparation method of a microbial agent and a preparation method of fermented bean curd, and relates to the technical field of microorganisms. The invention relates to a lactobacillus paracasei CN23, which is preserved in the China General Microbiological Culture Collection Center on September 2, 2024, and the preservation number is CGMCC NO. 31794. The lactobacillus paracasei CN23 is named as the lactobacillus paracasei CN23. The fermented bean curd prepared by fermenting the acid water with the microbial agent containing the casei paracasei CN23 is higher in total acid content and amino acid nitrogen content, richer and more complex in flavor and rich in layering sense, the total biogenic amine content and the white dot ratio are remarkably reduced, and the fermented bean curd has the advantages of being good in taste and flavor, rich in nutrition, good in taste and rich in flavor. The fermented bean curd has the characteristics of no food safety risk, no preservative and coagulator, and significantly improves the quality of the fermented bean curd.
Owner:CHENGDU MOLECULAR POWER BIOTECHNOLOGY CO LTD +1

Fluorescent tag for non-destructive visual monitoring of freshness of fish and meat food products and method for its preparation

The application discloses a fluorescent label for nondestructive visual monitoring of freshness of fish and meat food and a preparation method thereof. The fluorescent label is characterized in that a key fluorescent substance JDCN specifically interacts with volatile biogenic amines generated in the meat spoilage process, color and fluorescence double optical signal changes occur, the biogenic amine content is judged according to the visual optical characteristics, and thus the freshness of the fish and meat food is monitored. The application has the characteristics of simple preparation, low cost, high sensitivity, high selectivity, fast visual response, and the like, can in-situ rapidly detect the volatile biogenic amines generated in the spoilage of the fish and meat food, provides a simple, convenient and effective means for the rapid visual detection of the freshness of the fish and meat food, is expected to become a preferred method for rapid detection of the content of the volatile biogenic amines in the fish and meat food, provides a powerful technical guarantee for self-monitoring of food manufacturers and market supervision, has great application prospect, and is of great popularization significance.
Owner:GUANGXI UNIV

Hazelnut meal fermented product as well as preparation method and application thereof

The invention relates to the technical field of fermentation, in particular to a hazelnut meal fermented product as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: inoculating Leuconostoc mesenteroides into fermentation liquor containing hazelnut meal, and fermenting, so as to obtain a hazelnut meal fermentation product; the Leuconostoc mesenteroides is preserved in the China Center for Type Culture Collection, and the preservation number of the Leuconostoc mesenteroides is CCTCC (China Center for Type Culture Collection) NO: M2024452. According to the invention, specific leuconostoc mesenteroides is utilized, tyrosine, phenylalanine and the like in metabolites obtained by fermentation are precursors for synthesizing neurotransmitters, and in addition, some biogenic amines obtained by fermentation have a synergistic refreshing function. Meanwhile, in the fermentation process, protein can be decomposed into amino acid and polypeptide, a Maillard reaction is carried out to generate pyrazine substances with flavors of baking, nuts and the like, and the flavor is further enriched by the flavors generated by fermentation of other protein.
Owner:LIAONING INST OF ECONOMIC FORESTRY +1

Self-assembled supramolecular peptide nanoscale enzyme material, and preparation method and application thereof

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.
Owner:BEIJING TECH & BUSINESS UNIV

SERS (Surface Enhanced Raman Scattering) aptamer sensor based on double-layer core-satellite magnetic gold nanostructure and method for detecting biogenic amine in food by using SERS aptamer sensor

The invention discloses an SERS (Surface Enhanced Raman Scattering) aptamer sensor based on a double-layer core-satellite magnetic gold nanostructure and a method for detecting biogenic amine in food by using the SERS aptamer sensor. The Fe3O4 (at) Au MNPs nanoparticles are combined with a nucleic acid aptamer, and a specific magnetic capture probe is obtained; the method comprises the following steps: mixing gold nanoparticle solutions with different particle sizes with 4-MBN, incubating to obtain a'biological silence zone 'SERS tag, and combining the'biological silence zone' SERS tag with pretreated sulfydryl modified c-DNA to obtain a specific SERS signal probe; and oscillating and incubating the specific SERS signal probe solution and the specific magnetic capture probe solution to obtain the SERS aptamer sensor based on the double-layer core-satellite magnetic gold nanostructure. According to the method, a'biological silence zone 'SERS label is combined with a magnetic induction technology, so that the problems of poor anti-interference performance and poor accuracy during detection of trace components in a complex matrix by SERS are solved, and high-efficiency detection of tryptamine in biogenic amine is realized.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Natural sweet wine low-sulfur brewing method for harvesting white grapes after white grapes turn red

The invention belongs to the technical field of wine brewing, relates to a natural sweet wine low-sulfur brewing method for delaying white grapes until the white grapes turn red and then harvesting the white grapes, solves the health hidden danger and flavor defects caused by high-sulfur addition in the traditional sweet white wine process, and remarkably improves the flavor quality of the wine at the same time. According to the method, complete and healthy red (total anthocyanin content is greater than or equal to 5mg / kg) fruits are selected through artificial spike selection and grain selection, and the cardinal number of infectious microbes in raw materials is reduced; no commercial yeast is added, natural slow fermentation is achieved by means of microorganisms, and the finished product is good in flavor and quality; gallic acid, condensed tannin and hydrolyzed tannin with a ratio of 1: 1: 1 are compounded to prepare wine-brewing tannin, and the wine-brewing tannin and low-dose sulfur dioxide construct a synergistic system, so that the sulfur consumption of sulfur dioxide is greatly reduced, secondary fermentation of high-sugar wine liquid is prevented, and infectious microbes and wine body oxidation are effectively inhibited. The finished product biogenic amine, pesticide and heavy metal residues all meet the safety standard, mycotoxin is not detected, meanwhile, the original flavor of the wine body is prominent, the storage stability is good, and dual guarantee of health and quality is achieved.
Owner:NINGXIA UNIVERSITY

Lactobacillus plantarum with overexpressed luxS gene, microbial preparation and application of lactobacillus plantarum

The invention discloses lactobacillus plantarum for overexpressing a luxS gene, a microbial preparation and application of the lactobacillus plantarum, and belongs to the technical field of microorganisms. In order to solve the technical problem that excessive biogenic amines are accumulated in a fermented meat product in the prior art, the invention provides lactobacillus plantarum for overexpressing a luxS gene and a microbial preparation containing the lactobacillus plantarum. The lactobacillus plantarum for overexpressing the luxS gene, provided by the invention, has the capability of highly producing signal molecules AI-2 and has the capability of remarkably improving the activities of diamine oxidase and polyketide oxidase; the lactobacillus plantarum overexpressing the luxS gene and the microbial preparation containing the lactobacillus plantarum can be used as a leavening agent for reducing biogenic amine to be applied to production of fermented meat products, the content of biogenic amine in the air-dried sausages can be effectively reduced, and the quality and safety of the air-dried sausages are improved.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of multi-copper oxidase mutant

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.
Owner:FUJIAN NORMAL UNIV

Composite nanofiltration membrane and preparation method thereof

The invention discloses a composite nanofiltration membrane which comprises a base membrane and a functional layer, the functional layer comprises a polymer, polymerization unit monomers of the polymer comprise amine monomers and cyanuric chloride derivatives, the amine monomers comprise aliphatic amine and aromatic amine, the aliphatic amine comprises at least three primary amine or secondary amine groups, and the aromatic amine comprises at least three secondary amine groups. The cyanuric chloride derivative comprises at least one of a compound shown in a formula I and a compound shown in a formula II, and # imgabs0. The nanofiltration membrane provided by the invention has relatively high water flux.
Owner:CHINA LUCKY GROUP CORP

Organic Field-Effect Transistor Gas Sensor, Odor Measurement Circuit and Method

The present invention belongs to the technical field of food biogenic amine detection, and discloses an organic field effect transistor gas sensor, an odor measurement circuit and a method. According to the present invention, the organic field effect transistor and the odor detection circuit sense food biogenic amine, causing changes in the internal characteristics of the organic field effect transistor, which in turn leads to changes in the circuit voltage signal. The voltage gain of the feedback circuit is adjusted according to the voltage signal displayed on the oscilloscope, so that the voltage displayed on the oscilloscope is equal to the source voltage, realizing the adjustment of the voltage gain by the mobile terminal. Based on the relationship between the source voltage and the voltage gain, the changes in the charge carrier mobility and the threshold voltage are determined. Thus, the detection result of food biogenic amine is obtained according to the changes in the charge carrier mobility or the threshold voltage, achieving the purpose of rapid on-site detection.
Owner:WUHAN POLYTECHNIC UNIVERSITY

A carbonyl-containing coupling rigid fragment compound, a preparation method and a sterilization use

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.
Owner:EAST CHINA UNIV OF SCI & TECH

Hansenula polymorpha cloud-268, application thereof, dry red wine fermentation method and microbial agent

The invention relates to the technical field of wine brewing, in particular to hansenula polymorpha cloud-268, application of the hansenula polymorpha cloud, a fermentation method of dry red wine and a microbial agent. The hansenula polymorpha var. Uvarum cloud-268 is preserved in the China Center for Type Culture Collection on December 13, 2013, and the preservation number of the hansenula polymorpha var. Uvarum cloud-268 is CCTCC NO: M2013658. According to the invention, non-saccharomyces cerevisiae YUN-268 and saccharomyces cerevisiae are preferably selected for synergistic fermentation, and the stability of a fermentation system is maintained by using the fermentation biological protection effect of the non-saccharomyces cerevisiae. Accumulation of potential harmful organism amine is remarkably reduced, generation of aroma substances of the grape wine is promoted, and the aroma of the obtained grape wine is richer. The method provides a feasible and efficient technical scheme for green brewing of low-sulfur and high-quality wine, has the advantages of greenness, safety and high quality, and provides a technical path capable of being popularized for industrial production of the low-sulfur dry red wine.
Owner:NORTHWEST A & F UNIV +1

Methods and compositions for suppression of biogenic amines produced by the gut microbiome

The present invention provides for methods of treating biogenic amine related conditions. Further provided are methods of detecting biogenic amine related conditions, and selection of treatment for patients in need thereof.
Owner:CEDARS SINAI MEDICAL CENT

Pediococcus pentosaceus Ppee-YC39 capable of enhancing nutritional activity and flavor of fish tea and inhibiting formation of biogenic amine and application of Pediococcus pentosaceus Ppee-YC39

The invention discloses pediococcus pentosaceus Ppe-YC39 capable of enhancing the nutritional activity and flavor of fish tea and inhibiting formation of biogenic amine and application of the pediococcus pentosaceus Ppe-YC39. Wherein the pediococcus pentosaceus Ppe-YC39 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the pediococcus pentosaceus Ppe-YC39 is GDMCC No: 66920. The pediococcus pentosaceus Ppe-YC39 disclosed by the invention has a probiotic attribute equivalent to or even better than that of a commercial strain lactobacillus plantarum LGG, and when the pediococcus pentosaceus Ppe-YC39 is applied to fish tea fermentation, the content of amino acid and fatty acid in the fermented fish tea can be remarkably increased, and the content of biogenic amine is remarkably reduced.
Owner:SHENZHEN UNIV

Non-mycelium-producing rouxellia yeast capable of inhibiting mycelium-producing microorganisms, microbial inoculant and application thereof

The application discloses a non-membrane-producing Rhodotorula conjugata capable of inhibiting membrane-producing microorganisms, a microbial inoculum and application, relates to the technical field of microorganisms and food fermentation, and the Rhodotorula conjugata (KDX-69) is preserved in the Guangdong Microbial Culture Collection Center on Nov. 14, 2025, and has a preservation number of GDMCC No: 67296. Zygosaccharomyces rouxii The new strain is obtained from a bean paste, does not produce membranes by itself, and can inhibit the growth of membrane-producing microorganisms; the new strain is applied to a low-salt fresh pepper bean paste fermentation process, can effectively inhibit membrane production, flower production and other spoilage and deterioration, reduce the content of biological amine in a product, improve product safety, improve product flavor, and has high application value.
Owner:SICHUAN KOUDAXIANG FOOD CO LTD +1

Preparation process of rice wine fermentation

PendingCN122648186ABiofilmDigestion Treatment
The application discloses a preparation process of rice wine fermentation and relates to the technical field of rice wine preparation, and comprises the following steps: S1, a circulating fermentation activator is prepared; S2, the circulating fermentation activator is introduced into a digestion treatment procedure, a porous carrier is loaded with non-amine-producing competitive lactic acid bacteria biofilm and covalently immobilized amine oxidase; S3, according to the risk level of a coupling risk value, a corresponding digestion strengthening procedure is triggered; and S4, the circulating fermentation activator treated by the digestion treatment procedure is added back to a new batch of fermentation substrate. The application prepares the circulating fermentation activator by directional complex enzymolysis treatment of the distiller's grains generated after rice wine fermentation, and the non-amine-producing competitive lactic acid bacteria biofilm and the covalently immobilized amine oxidase are loaded on the porous carrier, so that the biological amine in the circulating system is subjected to directional digestion, and the problems of excessive accumulation of biological amine and deterioration of rice wine flavor caused by direct reuse of the distiller's grains in the prior art are solved.
Owner:JILIN DINGHONG TECHNOLOGY CO LTD

A nano intelligent indicating film with dual effects of meat preservation and freshness visualization and a preparation method thereof

The application discloses a kind of meat preservation and freshness visual double-effect nano intelligent indicating film and preparation method thereof, belong to food packaging storage and detection technical field.The preparation method of the intelligent indicating film includes the following steps: (1) poly-3-hydroxybutyric acid-co-oxyvalerate (PHBV) and ferulic acid are dissolved in chloroform solution;(2) polyvinyl alcohol (PVA), konjac glucomannan (KGM), alizarin (AL) are mixed, and polyvinyl alcohol-konjac glucomannan-alizarin mixed solution is obtained by removing bubble;(3) PH-F solution is dried by electrospinning technology, and PVA-KGM-AL@PH-F double-layer indicating film is obtained.The application prepares meat freshness indicating film with efficient antibacterial performance and high sensitivity color response performance by regulating the pH of film-forming solution.In the intelligent indicating film, alizarin responds to meat spoilage biogenic amine, and the color of indicating film gradually changes from cream (slightly yellow) to light pink or even deep purple;By comparing the intelligent indicating film after reflection with colorimetric card, the freshness of meat can be judged.
Owner:CHENGDU UNIV

Brewing method of yellow wine

The invention discloses a brewing method of yellow rice wine, which adopts lactobacillus sake L.48 with the preservation number of CCTCC NO: M 20221306, the strain deletes histidine decarboxylase gene, the CRISPR (clustered regularly interspaced short palindromic repeats) repetition number is 39, and biogenic amine can be obviously reduced. According to the process, rice cleaned with warm water is taken as a raw material, lactobacillus sake L.48 is inoculated for co-fermentation with yeast and distiller's yeast, primary fermentation is performed at 25-32 DEG C, secondary fermentation is performed at 18-25 DEG C, and the process is completed within 23 days. Compared with a traditional rice soaking method, the production period is shortened, the biogenic amine content in the product is reduced by more than or equal to 60%, and the ester aroma is improved by 2-3 times.
Owner:HEFEI UNIV OF TECH

Liquid mass spectrometry detection method for biogenic amine in bee tissue

The invention discloses a liquid mass spectrometry detection method for biogenic amines in bee tissues, and relates to the technical field of compound detection. The detection method comprises the following steps: dissecting to obtain a bee brain tissue sample (with a single eye and a compound eye removed), putting the sample into a centrifugal tube, adding an extracting solution, namely a hydrochloric acid aqueous solution, crushing the tissue crushed sample by an electric grinder, carrying out ultrasonic treatment on precipitated protein, transferring supernate into a filter type sample bottle, filtering, and carrying out liquid chromatography-mass spectrometry determination. The invention establishes a method for simultaneously and rapidly detecting four biogenic amines in a bee tissue sample by HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry), the method can simultaneously analyze four chemical substances (biogenic amines) within 8 minutes, the detection time is short, the linear relation is better, the method recovery rate is high, the stability is good, and the analysis requirement is met.
Owner:JIANGXI APICULTURE RES INST

Bifidobacterium longum subsp. Infantis, probiotic preparation and application of probiotic preparation in hypoglycemic and hypolipidemic products

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Infantis, a probiotic preparation and application of the probiotic preparation in products for reducing blood sugar and blood lipid. The bifidobacterium longum subsp. Infantis provided by the invention is bifidobacterium longum subsp. Infantis A32-03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32811. The strain does not generate hemolysin, does not have nitrate reductase activity, does not generate any harmful metabolites such as biogenic amines and indoles, is sensitive to various antibiotics, and is good in biological safety. Particularly, the probiotic preparation obtained by fermenting the bifidobacterium longum subsp. Infantis A32-03 in a culture medium containing the auricularia auricula polysaccharide has a prominent inhibition effect on alpha-amylase, alpha-glucosidase and lipase, has an excellent degradation capability on cholesterol, has a good blood sugar and blood fat regulation effect, and can be used for preparing the probiotic preparation. The method has good popularization value in oral administration of medicines for reducing blood sugar and blood fat or health-care food for regulating blood sugar and blood fat.
Owner:1 HEILONGJIANG GREEN FOOD SCIENCE RESEARCH INSTITUTE +2

Low-temperature cured bio-based high-toughness epoxy asphalt material and preparation method thereof

A low-temperature cured bio-based high-toughness epoxy asphalt material relates to the technical field of preparation of road engineering materials, and is prepared from the following raw materials in parts by mass: bisphenol A type epoxy resin, bio-based epoxy resin, a biogenic amine curing agent, an epoxy diluent, a catalyst and matrix asphalt. The asphalt comprises the following components in parts by weight: 15-30 parts of bisphenol A type epoxy resin, 5-18 parts of bio-based epoxy resin, 10-20 parts of a biogenic amine curing agent, 1-5 parts of an epoxy diluent, 0.5-3 parts of a catalyst and 35-55 parts of matrix asphalt. The preparation method of the low-temperature cured bio-based high-toughness epoxy asphalt material comprises four steps. The preparation raw materials mainly come from renewable biomass resources, the production does not depend on non-renewable petroleum resources, and the prepared finished product has good degradability, reduces the risk of environmental pollution, has certain chemical stability and physical properties, and is suitable for industrial production. The pavement performance such as high-temperature rut resistance, low-temperature crack resistance and water damage resistance of the road asphalt mixture can be improved, and the overall quality of the road mixture can be improved.
Owner:ANZHENGHANG NEW MATERIALS TECHNOLOGY (SHANGHAI) CO LTD

A rare earth metal-based ternary composite antibacterial agent and its preparation method

This invention provides a rare earth metal-based ternary composite antibacterial agent and its preparation method. The antibacterial agent is prepared from raw materials including GL-22, Eu-containing polyoxometalate clusters, and Spm. The rare earth metal-based ternary composite antibacterial agent of this invention, after fractional compounding of Eu-containing polyoxometalate clusters with peptides and biogenic amines, exhibits highly efficient antibacterial function against Escherichia coli and Staphylococcus aureus. The ternary assembly EuW... 10 The fluorescence emission of / GL-22 / Spm is enhanced by 30-40 times, and it is effective against Escherichia coli (E. coli). E. coli The antibacterial properties of the drug have been increased from 40%~30% to 90%~95%, and its efficacy against bacteria has been improved. E. coli The inhibition rate of biofilm (BME) formation was 21.8%.
Owner:TIANJIN BAOGANG RES INST OF RARE EARTHS CO LTD

Fluorescent probe for detecting cadaverine as well as preparation method and application of fluorescent probe

In meat freshness evaluation, biogenic amines, especially cadaverine, are key indexes. The novel fluorescent probe ZY1 provided by the invention reacts with cadaverine to realize efficient and rapid detection of meat freshness. The probe has excellent selectivity and ultralow detection limit (LDD = 13.8 nM), and is accompanied by remarkable fluorescence color conversion (light red to bright green). Based on the characteristic, the ZY1 is innovatively integrated with RGB analysis software of a smart phone, and a reliable linear relation between a G / B value and food preservation time is established by shooting a meat extracting solution loaded with the ZY1, so that real-time and portable detection of the food freshness is realized. In view of specific up-regulation of cadaverine biosynthesis in ulcerative colitis (UC), the research further proves the application potential of ZY1 in UC noninvasive auxiliary diagnosis. In addition, ZY1 also shows expanded application in the technical fields of preparation of novel fluorescent materials and information transmission.
Owner:CHONGQING MEDICAL UNIVERSITY