88 results about "Biogenic amine" patented technology

Chickpea with peel is used as a main raw material, black rice and quinoa are compounded, pretreatment is performed through an ultrasonic-assisted enzyme method, efficient softening is achieved, and a three-stage mixed fermentation process is adopted: in the first stage, lactic acid bacteria are utilized for pre-fermentation for acidification, infectious microbes are inhibited, and generation of biogenic amine is controlled; in the second stage, carrying out gradient temperature fermentation by adopting the danbei fungus powder, firstly promoting mycelial growth at low temperature, and then exciting a Maillard reaction at high temperature to improve the flavor; in the third stage, probiotic microcapsules are added for after-ripening, biogenic amine is further degraded, the flavor is improved, the product is endowed with probiotic activity, and a bacteriostatic protective layer is provided. The method successfully solves the problem that the texture is rough due to the fact that the chickpea skin is reserved, and the final product has the comprehensive advantages of being rich in nutrition, good in taste, high in safety and strong in probiotic function and is high in sensory acceptability.

The invention relates to a porphyrin metal organic framework composite material, a preparation method and a biogenic amine SERS (Surface Enhanced Raman Scattering) array sensor. The preparation method of the composite material comprises the steps that a copper precursor solution is added into a surfactant, heating reaction is conducted, a reducing agent is added, heating reaction is conducted, and copper nanoparticles are obtained; the preparation method comprises the following steps: adding a transition metal salt and a surfactant into an organic solvent, adding a porphyrin ligand, carrying out a solvothermal reaction, carrying out centrifugal separation, washing and drying to obtain a porphyrin metal organic framework material, and dispersing the porphyrin metal organic framework material into the organic solvent to obtain a dispersion liquid A; and dispersing copper nanoparticles in the dispersion liquid A, carrying out a second heating reaction, and carrying out centrifugal separation, washing and drying to obtain the porphyrin metal organic framework composite material. The invention also provides the porphyrin metal organic framework composite material prepared by the preparation method, and the application of the porphyrin metal organic framework composite material as a biogenic amine SERS array sensor. The invention solves the problem that the accuracy of the detection result is influenced because the traditional biogenic amine detection method is difficult to realize field detection.

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

The invention relates to a pet food and a processing method thereof, and relates to the technical field of pet food processing, the pet food comprises the following components in parts by mass: meat meal, prebiotics, animal fat, a mineral additive, a vitamin additive, an edible binder, and a biogenic amine complexation-decarboxylase inhibitor; the processing method comprises the steps of raw material mixing and forming. According to the pet food and the processing method thereof, on the premise that basic nutrition needed by growth and development of pets is met, the effects of blocking generation of biogenic amine, complexing generated biogenic amine and reducing the content of volatile basic nitrogen are achieved, meanwhile, the palatability and storage stability of the pet food are improved, and the pet food is suitable for popularization and application. And the health risks of intestinal discomfort and the like caused by ingestion of biogenic amine by pets are reduced.

The application discloses a fluorescent label for nondestructive visual monitoring of freshness of fish and meat food and a preparation method thereof. The fluorescent label is characterized in that a key fluorescent substance JDCN specifically interacts with volatile biogenic amines generated in the meat spoilage process, color and fluorescence double optical signal changes occur, the biogenic amine content is judged according to the visual optical characteristics, and thus the freshness of the fish and meat food is monitored. The application has the characteristics of simple preparation, low cost, high sensitivity, high selectivity, fast visual response, and the like, can in-situ rapidly detect the volatile biogenic amines generated in the spoilage of the fish and meat food, provides a simple, convenient and effective means for the rapid visual detection of the freshness of the fish and meat food, is expected to become a preferred method for rapid detection of the content of the volatile biogenic amines in the fish and meat food, provides a powerful technical guarantee for self-monitoring of food manufacturers and market supervision, has great application prospect, and is of great popularization significance.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention discloses an SERS (Surface Enhanced Raman Scattering) aptamer sensor based on a double-layer core-satellite magnetic gold nanostructure and a method for detecting biogenic amine in food by using the SERS aptamer sensor. The Fe3O4 (at) Au MNPs nanoparticles are combined with a nucleic acid aptamer, and a specific magnetic capture probe is obtained; the method comprises the following steps: mixing gold nanoparticle solutions with different particle sizes with 4-MBN, incubating to obtain a'biological silence zone 'SERS tag, and combining the'biological silence zone' SERS tag with pretreated sulfydryl modified c-DNA to obtain a specific SERS signal probe; and oscillating and incubating the specific SERS signal probe solution and the specific magnetic capture probe solution to obtain the SERS aptamer sensor based on the double-layer core-satellite magnetic gold nanostructure. According to the method, a'biological silence zone 'SERS label is combined with a magnetic induction technology, so that the problems of poor anti-interference performance and poor accuracy during detection of trace components in a complex matrix by SERS are solved, and high-efficiency detection of tryptamine in biogenic amine is realized.

The invention belongs to the technical field of wine brewing, relates to a natural sweet wine low-sulfur brewing method for delaying white grapes until the white grapes turn red and then harvesting the white grapes, solves the health hidden danger and flavor defects caused by high-sulfur addition in the traditional sweet white wine process, and remarkably improves the flavor quality of the wine at the same time. According to the method, complete and healthy red (total anthocyanin content is greater than or equal to 5mg / kg) fruits are selected through artificial spike selection and grain selection, and the cardinal number of infectious microbes in raw materials is reduced; no commercial yeast is added, natural slow fermentation is achieved by means of microorganisms, and the finished product is good in flavor and quality; gallic acid, condensed tannin and hydrolyzed tannin with a ratio of 1: 1: 1 are compounded to prepare wine-brewing tannin, and the wine-brewing tannin and low-dose sulfur dioxide construct a synergistic system, so that the sulfur consumption of sulfur dioxide is greatly reduced, secondary fermentation of high-sugar wine liquid is prevented, and infectious microbes and wine body oxidation are effectively inhibited. The finished product biogenic amine, pesticide and heavy metal residues all meet the safety standard, mycotoxin is not detected, meanwhile, the original flavor of the wine body is prominent, the storage stability is good, and dual guarantee of health and quality is achieved.

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The invention relates to the technical field of wine brewing, in particular to hansenula polymorpha cloud-268, application of the hansenula polymorpha cloud, a fermentation method of dry red wine and a microbial agent. The hansenula polymorpha var. Uvarum cloud-268 is preserved in the China Center for Type Culture Collection on December 13, 2013, and the preservation number of the hansenula polymorpha var. Uvarum cloud-268 is CCTCC NO: M2013658. According to the invention, non-saccharomyces cerevisiae YUN-268 and saccharomyces cerevisiae are preferably selected for synergistic fermentation, and the stability of a fermentation system is maintained by using the fermentation biological protection effect of the non-saccharomyces cerevisiae. Accumulation of potential harmful organism amine is remarkably reduced, generation of aroma substances of the grape wine is promoted, and the aroma of the obtained grape wine is richer. The method provides a feasible and efficient technical scheme for green brewing of low-sulfur and high-quality wine, has the advantages of greenness, safety and high quality, and provides a technical path capable of being popularized for industrial production of the low-sulfur dry red wine.

The invention discloses pediococcus pentosaceus Ppe-YC39 capable of enhancing the nutritional activity and flavor of fish tea and inhibiting formation of biogenic amine and application of the pediococcus pentosaceus Ppe-YC39. Wherein the pediococcus pentosaceus Ppe-YC39 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the pediococcus pentosaceus Ppe-YC39 is GDMCC No: 66920. The pediococcus pentosaceus Ppe-YC39 disclosed by the invention has a probiotic attribute equivalent to or even better than that of a commercial strain lactobacillus plantarum LGG, and when the pediococcus pentosaceus Ppe-YC39 is applied to fish tea fermentation, the content of amino acid and fatty acid in the fermented fish tea can be remarkably increased, and the content of biogenic amine is remarkably reduced.

The application discloses a non-membrane-producing Rhodotorula conjugata capable of inhibiting membrane-producing microorganisms, a microbial inoculum and application, relates to the technical field of microorganisms and food fermentation, and the Rhodotorula conjugata (KDX-69) is preserved in the Guangdong Microbial Culture Collection Center on Nov. 14, 2025, and has a preservation number of GDMCC No: 67296. Zygosaccharomyces rouxii The new strain is obtained from a bean paste, does not produce membranes by itself, and can inhibit the growth of membrane-producing microorganisms; the new strain is applied to a low-salt fresh pepper bean paste fermentation process, can effectively inhibit membrane production, flower production and other spoilage and deterioration, reduce the content of biological amine in a product, improve product safety, improve product flavor, and has high application value.

The application discloses a preparation process of rice wine fermentation and relates to the technical field of rice wine preparation, and comprises the following steps: S1, a circulating fermentation activator is prepared; S2, the circulating fermentation activator is introduced into a digestion treatment procedure, a porous carrier is loaded with non-amine-producing competitive lactic acid bacteria biofilm and covalently immobilized amine oxidase; S3, according to the risk level of a coupling risk value, a corresponding digestion strengthening procedure is triggered; and S4, the circulating fermentation activator treated by the digestion treatment procedure is added back to a new batch of fermentation substrate. The application prepares the circulating fermentation activator by directional complex enzymolysis treatment of the distiller's grains generated after rice wine fermentation, and the non-amine-producing competitive lactic acid bacteria biofilm and the covalently immobilized amine oxidase are loaded on the porous carrier, so that the biological amine in the circulating system is subjected to directional digestion, and the problems of excessive accumulation of biological amine and deterioration of rice wine flavor caused by direct reuse of the distiller's grains in the prior art are solved.

The application discloses a kind of meat preservation and freshness visual double-effect nano intelligent indicating film and preparation method thereof, belong to food packaging storage and detection technical field.The preparation method of the intelligent indicating film includes the following steps: (1) poly-3-hydroxybutyric acid-co-oxyvalerate (PHBV) and ferulic acid are dissolved in chloroform solution;(2) polyvinyl alcohol (PVA), konjac glucomannan (KGM), alizarin (AL) are mixed, and polyvinyl alcohol-konjac glucomannan-alizarin mixed solution is obtained by removing bubble;(3) PH-F solution is dried by electrospinning technology, and PVA-KGM-AL@PH-F double-layer indicating film is obtained.The application prepares meat freshness indicating film with efficient antibacterial performance and high sensitivity color response performance by regulating the pH of film-forming solution.In the intelligent indicating film, alizarin responds to meat spoilage biogenic amine, and the color of indicating film gradually changes from cream (slightly yellow) to light pink or even deep purple;By comparing the intelligent indicating film after reflection with colorimetric card, the freshness of meat can be judged.

The invention discloses a brewing method of yellow rice wine, which adopts lactobacillus sake L.48 with the preservation number of CCTCC NO: M 20221306, the strain deletes histidine decarboxylase gene, the CRISPR (clustered regularly interspaced short palindromic repeats) repetition number is 39, and biogenic amine can be obviously reduced. According to the process, rice cleaned with warm water is taken as a raw material, lactobacillus sake L.48 is inoculated for co-fermentation with yeast and distiller's yeast, primary fermentation is performed at 25-32 DEG C, secondary fermentation is performed at 18-25 DEG C, and the process is completed within 23 days. Compared with a traditional rice soaking method, the production period is shortened, the biogenic amine content in the product is reduced by more than or equal to 60%, and the ester aroma is improved by 2-3 times.

The invention discloses a liquid mass spectrometry detection method for biogenic amines in bee tissues, and relates to the technical field of compound detection. The detection method comprises the following steps: dissecting to obtain a bee brain tissue sample (with a single eye and a compound eye removed), putting the sample into a centrifugal tube, adding an extracting solution, namely a hydrochloric acid aqueous solution, crushing the tissue crushed sample by an electric grinder, carrying out ultrasonic treatment on precipitated protein, transferring supernate into a filter type sample bottle, filtering, and carrying out liquid chromatography-mass spectrometry determination. The invention establishes a method for simultaneously and rapidly detecting four biogenic amines in a bee tissue sample by HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry), the method can simultaneously analyze four chemical substances (biogenic amines) within 8 minutes, the detection time is short, the linear relation is better, the method recovery rate is high, the stability is good, and the analysis requirement is met.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Infantis, a probiotic preparation and application of the probiotic preparation in products for reducing blood sugar and blood lipid. The bifidobacterium longum subsp. Infantis provided by the invention is bifidobacterium longum subsp. Infantis A32-03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32811. The strain does not generate hemolysin, does not have nitrate reductase activity, does not generate any harmful metabolites such as biogenic amines and indoles, is sensitive to various antibiotics, and is good in biological safety. Particularly, the probiotic preparation obtained by fermenting the bifidobacterium longum subsp. Infantis A32-03 in a culture medium containing the auricularia auricula polysaccharide has a prominent inhibition effect on alpha-amylase, alpha-glucosidase and lipase, has an excellent degradation capability on cholesterol, has a good blood sugar and blood fat regulation effect, and can be used for preparing the probiotic preparation. The method has good popularization value in oral administration of medicines for reducing blood sugar and blood fat or health-care food for regulating blood sugar and blood fat.

This invention provides a rare earth metal-based ternary composite antibacterial agent and its preparation method. The antibacterial agent is prepared from raw materials including GL-22, Eu-containing polyoxometalate clusters, and Spm. The rare earth metal-based ternary composite antibacterial agent of this invention, after fractional compounding of Eu-containing polyoxometalate clusters with peptides and biogenic amines, exhibits highly efficient antibacterial function against Escherichia coli and Staphylococcus aureus. The ternary assembly EuW... 10 The fluorescence emission of / GL-22 / Spm is enhanced by 30-40 times, and it is effective against Escherichia coli (E. coli). E. coli The antibacterial properties of the drug have been increased from 40%~30% to 90%~95%, and its efficacy against bacteria has been improved. E. coli The inhibition rate of biofilm (BME) formation was 21.8%.

In meat freshness evaluation, biogenic amines, especially cadaverine, are key indexes. The novel fluorescent probe ZY1 provided by the invention reacts with cadaverine to realize efficient and rapid detection of meat freshness. The probe has excellent selectivity and ultralow detection limit (LDD = 13.8 nM), and is accompanied by remarkable fluorescence color conversion (light red to bright green). Based on the characteristic, the ZY1 is innovatively integrated with RGB analysis software of a smart phone, and a reliable linear relation between a G / B value and food preservation time is established by shooting a meat extracting solution loaded with the ZY1, so that real-time and portable detection of the food freshness is realized. In view of specific up-regulation of cadaverine biosynthesis in ulcerative colitis (UC), the research further proves the application potential of ZY1 in UC noninvasive auxiliary diagnosis. In addition, ZY1 also shows expanded application in the technical fields of preparation of novel fluorescent materials and information transmission.

This invention discloses a method for preparing a highly antioxidant, color-changing, intelligent active food preservation film containing bilberry anthocyanins, belonging to the field of food preservation technology. An ACNs-CHC / CMC-WPI nanocomposite is constructed through the encapsulation of chitosan hydrochloride (CHC), carboxymethyl chitosan (CMC), and whey protein isolate (WPI). Using corn starch, chitosan, and 30 wt% glycerol as a base material, the anthocyanin-loaded nanocomposite is introduced as a functional component and formed on the surface of an acrylic sheet via solution casting. This food preservation film exhibits excellent mechanical properties and effectively inhibits spoilage and the proliferation of pathogenic bacteria in high-protein aquatic foods (such as white shrimp), reduces the formation rate of biogenic amines, and significantly slows down the deterioration process of food quality. Furthermore, the surface color of the film changes with the pH value of the target meat, providing a visual monitoring method for food freshness and can also be used for rapid detection of food freshness.

The invention discloses a camellia chicken source bacillus velezensis CH3-2 and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis CH3-2 obtained through separation shows high tolerance to acid and cholate, has excellent adhesion capacity, and shows broad-spectrum antibacterial and excellent antioxidant activity. Safety evaluation proves that the strain CH3-2 has no hemolytic activity, does not generate biogenic amine and does not contain virulence genes. Six antibiotic resistance genes which are inherent and coded by chromosomes and carried by the gene have no risk of horizontal transfer. A mouse acute oral toxicity test further verifies the in-vivo safety of the strain, the strain has no adverse effect on Kunming mouse tissues, and an excellent strain resource is provided for developing functional probiotic preparations or feed additives for poultry.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

The application discloses a kind of meat freshness rapid test paper, belong to food rapid detection technical field.The test paper is with water absorption filter paper as base material, and is loaded by the reaction color developing system consisting of o-Phthaldehyde, mercapto glycolic acid, sodium acetate buffer and fast blue B salt, can occur specific color reaction with the biological amine (putrescine) generated in the meat spoilage process, and realizes freshness rapid judgment by color ladder change.The detection time of the application is only 3-5 minutes, without large instrument, simple operation, high sensitivity, low cost, wide spectrum, can be used for on-site rapid screening of pork, beef, mutton, frozen shrimp, frozen chicken breast and other various meats, and is widely applicable to raw material acceptance of meat processing enterprises, market supervision law enforcement and cold chain logistics quality monitoring.

The invention belongs to the technical field of feed processing, and particularly provides a preparation method of a bacterium-enzyme synergistic fermentation liquid feed, which comprises the following steps: adding water into feed raw materials, mixing, and adding a compound enzyme preparation for enzymolysis; the compound enzyme preparation comprises alkaline protease and xylanase; cooling the material liquid after enzymolysis, and inoculating a complex microbial inoculant for fermentation; the complex microbial inoculants comprise lactobacillus plantarum, enterococcus faecium and KM71 pichia pastoris; and after the fermentation is finished, sterilizing to obtain the bacterium-enzyme synergistic fermentation liquid feed. According to the preparation method provided by the invention, the pre-enzymolysis of macromolecular substances of the feed and the fermentation effect of microbial flora are cooperated, so that the content of nutrient substances in the feed is remarkably increased. Through sequential addition of an enzyme preparation and a microbial agent, two-stage precise temperature control fermentation and in-situ low-temperature pasteurization technology, fermentation is terminated on the premise of not destroying nutrition, and the problems of nutrition improvement, biogenic amine control and product shelf life are solved.

The invention discloses a lactobacillus plantarum YH-17 strain and an application of the lactobacillus plantarum YH-17 strain in wine brewing, and belongs to the technical field of microorganisms. The strain has the advantages of excellent alcohol and SO2 tolerance, prominent acid reduction capability, fast growth speed, high safety, no generation of biological amine, and no degradation of glycerin and citric acid. The strain is used for malic acid-lactic acid fermentation of wine, the fermentation speed is high, the deacidification effect is good, the aroma producing capacity is excellent, the aroma level of the wine can be enriched, the taste roundness is improved, and the problems of wine homogenization and high cost caused by current commercial Oenococcus oeni can be effectively solved.

The invention relates to the field of microorganisms, and provides a plant lactobacillus FVPGZP2101 and application thereof, the plant lactobacillus FVPGZP2101 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32871. The invention also provides a preparation method of the plant lactobacillus FVPGZP2101. The new strain of the plant lactobacillus obtained by screening has the advantages of strong growth ability, high acid production efficiency, strong acid resistance, capability of degrading nitrite, tryptamine, tyramine, spermidine and spermine, no generation of biogenic amine, no degradation of pectin, antibacterial activity on pathogenic bacteria, oxidation resistance of extracellular metabolites, and no pollution to the environment. According to the present invention, the new strain has advantages of high yield, key volatile substance production and non-volatile substance production, and the fermented food prepared by using the new strain has characteristics of low salt content, rich lactic acid bacteria content, nutrition, health and unique flavor; a food fermentation process developed by utilizing the new strain disclosed by the invention is high in fermentation efficiency, short in production period and high in application value.

The invention discloses a salt-tolerant Bacillus velezensis strain capable of efficiently degrading protein and biogenic amine and an application of the salt-tolerant Bacillus velezensis strain. The bacillus velezensis is a strain FJNU-SIO1, is classified and named as bacillus velezensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is September 26, 2025, the preservation number is CGMCC No.36086, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The strain FJNU-SIO1 is wide in growth adaptation range, can tolerate the salt concentration of 1%-10%, and can still efficiently degrade protein and nine common biogenic amines including putrescine, cadaverine, histamine and tyramine under the high salt concentration of 10%, the degradation rate of the protein can reach 47.68%, and the degradation rate of the total biogenic amines can reach 32.77%. The strain FJNU-SIO1 provided by the invention has the outstanding advantages of simplicity and convenience in operation, low cost, wide applicability and the like when being applied to degradation treatment of proteins and biogenic amines, and has an important value for guaranteeing safe production of fermented foods.

The invention belongs to the field of food packaging and food detection, and particularly relates to a method for preparing an amine response type nanofiber indicating film through electrostatic spinning and application of the amine response type nanofiber indicating film to food freshness detection. The method comprises the following steps that polycaprolactone serves as a film forming base material, polydiacetylene serves as an indicator, polyacrylic acid serves as an auxiliary agent, and the amine response type nanofiber indicating film is prepared through electrostatic spinning; the preparation method comprises the following steps: preparing a nanofiber membrane by adopting an electrostatic spinning technology, and preparing an amine response type nanofiber indication membrane through irradiation of an ultraviolet lamp; the prepared amine response type nanofiber indicating film has good biological safety and mechanical performance, the indicating film added with polydiacetylene and polyacrylic acid can generate obvious color change on low-concentration biogenic amine, and the freshness change condition of meat food such as shrimps in the transportation and storage period can be effectively indicated. The intelligent food freshness indicating film prepared by the method has wide application value in the field of intelligent food packaging.

The application discloses a compound Cl-BODIPY used as a fluorescent probe for rapid nondestructive detection of food freshness, and also establishes a corresponding preparation method and detection method. The probe produces a color and fluorescent dual optical signal change through specific interaction of a recognition group with volatile biogenic amines, and provides a simple and convenient detection method for rapid detection of volatile biogenic amines. The fluorescent probe is simple to prepare, low in cost, and has the characteristics of high sensitivity, high selectivity and rapid response, can in-situ rapidly detect volatile biogenic amines produced by fish and meat food spoilage, and judges the freshness (biogenic amine content) of fish and meat through colorimetric and fluorescent dual visual signal changes, thereby providing an effective means for rapid nondestructive detection of food freshness, having great application prospect and great popularization significance.