55 results about "Biogenic amine formation" patented technology

The invention relates to a porphyrin metal organic framework composite material, a preparation method and a biogenic amine SERS (Surface Enhanced Raman Scattering) array sensor. The preparation method of the composite material comprises the steps that a copper precursor solution is added into a surfactant, heating reaction is conducted, a reducing agent is added, heating reaction is conducted, and copper nanoparticles are obtained; the preparation method comprises the following steps: adding a transition metal salt and a surfactant into an organic solvent, adding a porphyrin ligand, carrying out a solvothermal reaction, carrying out centrifugal separation, washing and drying to obtain a porphyrin metal organic framework material, and dispersing the porphyrin metal organic framework material into the organic solvent to obtain a dispersion liquid A; and dispersing copper nanoparticles in the dispersion liquid A, carrying out a second heating reaction, and carrying out centrifugal separation, washing and drying to obtain the porphyrin metal organic framework composite material. The invention also provides the porphyrin metal organic framework composite material prepared by the preparation method, and the application of the porphyrin metal organic framework composite material as a biogenic amine SERS array sensor. The invention solves the problem that the accuracy of the detection result is influenced because the traditional biogenic amine detection method is difficult to realize field detection.

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

The application discloses a fluorescent label for nondestructive visual monitoring of freshness of fish and meat food and a preparation method thereof. The fluorescent label is characterized in that a key fluorescent substance JDCN specifically interacts with volatile biogenic amines generated in the meat spoilage process, color and fluorescence double optical signal changes occur, the biogenic amine content is judged according to the visual optical characteristics, and thus the freshness of the fish and meat food is monitored. The application has the characteristics of simple preparation, low cost, high sensitivity, high selectivity, fast visual response, and the like, can in-situ rapidly detect the volatile biogenic amines generated in the spoilage of the fish and meat food, provides a simple, convenient and effective means for the rapid visual detection of the freshness of the fish and meat food, is expected to become a preferred method for rapid detection of the content of the volatile biogenic amines in the fish and meat food, provides a powerful technical guarantee for self-monitoring of food manufacturers and market supervision, has great application prospect, and is of great popularization significance.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.

The invention relates to the technical field of wine brewing, in particular to hansenula polymorpha cloud-268, application of the hansenula polymorpha cloud, a fermentation method of dry red wine and a microbial agent. The hansenula polymorpha var. Uvarum cloud-268 is preserved in the China Center for Type Culture Collection on December 13, 2013, and the preservation number of the hansenula polymorpha var. Uvarum cloud-268 is CCTCC NO: M2013658. According to the invention, non-saccharomyces cerevisiae YUN-268 and saccharomyces cerevisiae are preferably selected for synergistic fermentation, and the stability of a fermentation system is maintained by using the fermentation biological protection effect of the non-saccharomyces cerevisiae. Accumulation of potential harmful organism amine is remarkably reduced, generation of aroma substances of the grape wine is promoted, and the aroma of the obtained grape wine is richer. The method provides a feasible and efficient technical scheme for green brewing of low-sulfur and high-quality wine, has the advantages of greenness, safety and high quality, and provides a technical path capable of being popularized for industrial production of the low-sulfur dry red wine.

The invention discloses pediococcus pentosaceus Ppe-YC39 capable of enhancing the nutritional activity and flavor of fish tea and inhibiting formation of biogenic amine and application of the pediococcus pentosaceus Ppe-YC39. Wherein the pediococcus pentosaceus Ppe-YC39 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the pediococcus pentosaceus Ppe-YC39 is GDMCC No: 66920. The pediococcus pentosaceus Ppe-YC39 disclosed by the invention has a probiotic attribute equivalent to or even better than that of a commercial strain lactobacillus plantarum LGG, and when the pediococcus pentosaceus Ppe-YC39 is applied to fish tea fermentation, the content of amino acid and fatty acid in the fermented fish tea can be remarkably increased, and the content of biogenic amine is remarkably reduced.

The application discloses a kind of meat preservation and freshness visual double-effect nano intelligent indicating film and preparation method thereof, belong to food packaging storage and detection technical field.The preparation method of the intelligent indicating film includes the following steps: (1) poly-3-hydroxybutyric acid-co-oxyvalerate (PHBV) and ferulic acid are dissolved in chloroform solution;(2) polyvinyl alcohol (PVA), konjac glucomannan (KGM), alizarin (AL) are mixed, and polyvinyl alcohol-konjac glucomannan-alizarin mixed solution is obtained by removing bubble;(3) PH-F solution is dried by electrospinning technology, and PVA-KGM-AL@PH-F double-layer indicating film is obtained.The application prepares meat freshness indicating film with efficient antibacterial performance and high sensitivity color response performance by regulating the pH of film-forming solution.In the intelligent indicating film, alizarin responds to meat spoilage biogenic amine, and the color of indicating film gradually changes from cream (slightly yellow) to light pink or even deep purple;By comparing the intelligent indicating film after reflection with colorimetric card, the freshness of meat can be judged.

The invention discloses a liquid mass spectrometry detection method for biogenic amines in bee tissues, and relates to the technical field of compound detection. The detection method comprises the following steps: dissecting to obtain a bee brain tissue sample (with a single eye and a compound eye removed), putting the sample into a centrifugal tube, adding an extracting solution, namely a hydrochloric acid aqueous solution, crushing the tissue crushed sample by an electric grinder, carrying out ultrasonic treatment on precipitated protein, transferring supernate into a filter type sample bottle, filtering, and carrying out liquid chromatography-mass spectrometry determination. The invention establishes a method for simultaneously and rapidly detecting four biogenic amines in a bee tissue sample by HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry), the method can simultaneously analyze four chemical substances (biogenic amines) within 8 minutes, the detection time is short, the linear relation is better, the method recovery rate is high, the stability is good, and the analysis requirement is met.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Infantis, a probiotic preparation and application of the probiotic preparation in products for reducing blood sugar and blood lipid. The bifidobacterium longum subsp. Infantis provided by the invention is bifidobacterium longum subsp. Infantis A32-03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32811. The strain does not generate hemolysin, does not have nitrate reductase activity, does not generate any harmful metabolites such as biogenic amines and indoles, is sensitive to various antibiotics, and is good in biological safety. Particularly, the probiotic preparation obtained by fermenting the bifidobacterium longum subsp. Infantis A32-03 in a culture medium containing the auricularia auricula polysaccharide has a prominent inhibition effect on alpha-amylase, alpha-glucosidase and lipase, has an excellent degradation capability on cholesterol, has a good blood sugar and blood fat regulation effect, and can be used for preparing the probiotic preparation. The method has good popularization value in oral administration of medicines for reducing blood sugar and blood fat or health-care food for regulating blood sugar and blood fat.

This invention provides a rare earth metal-based ternary composite antibacterial agent and its preparation method. The antibacterial agent is prepared from raw materials including GL-22, Eu-containing polyoxometalate clusters, and Spm. The rare earth metal-based ternary composite antibacterial agent of this invention, after fractional compounding of Eu-containing polyoxometalate clusters with peptides and biogenic amines, exhibits highly efficient antibacterial function against Escherichia coli and Staphylococcus aureus. The ternary assembly EuW... 10 The fluorescence emission of / GL-22 / Spm is enhanced by 30-40 times, and it is effective against Escherichia coli (E. coli). E. coli The antibacterial properties of the drug have been increased from 40%~30% to 90%~95%, and its efficacy against bacteria has been improved. E. coli The inhibition rate of biofilm (BME) formation was 21.8%.

This invention discloses a method for preparing a highly antioxidant, color-changing, intelligent active food preservation film containing bilberry anthocyanins, belonging to the field of food preservation technology. An ACNs-CHC / CMC-WPI nanocomposite is constructed through the encapsulation of chitosan hydrochloride (CHC), carboxymethyl chitosan (CMC), and whey protein isolate (WPI). Using corn starch, chitosan, and 30 wt% glycerol as a base material, the anthocyanin-loaded nanocomposite is introduced as a functional component and formed on the surface of an acrylic sheet via solution casting. This food preservation film exhibits excellent mechanical properties and effectively inhibits spoilage and the proliferation of pathogenic bacteria in high-protein aquatic foods (such as white shrimp), reduces the formation rate of biogenic amines, and significantly slows down the deterioration process of food quality. Furthermore, the surface color of the film changes with the pH value of the target meat, providing a visual monitoring method for food freshness and can also be used for rapid detection of food freshness.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The invention discloses application of a multi-copper oxidase mutant in biogenic amine degradation, and belongs to the technical field of food biology. The amino acid sequence of the multi-copper oxidase mutant is shown as SEQ ID NO.1. The application is to degrade biogenic amines in aquatic products, and the biogenic amines comprise tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine. The multi-copper oxidase mutant disclosed by the invention not only can effectively reduce the content of biogenic amines in aquatic products and reduce the generation of reaction byproducts, but also can maintain the freshness and nutritional value of the aquatic products, and has remarkable application value in the fields of aquatic product processing and food safety.

The invention relates to the field of microorganisms, and provides a plant lactobacillus FVPGZP2101 and application thereof, the plant lactobacillus FVPGZP2101 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32871. The invention also provides a preparation method of the plant lactobacillus FVPGZP2101. The new strain of the plant lactobacillus obtained by screening has the advantages of strong growth ability, high acid production efficiency, strong acid resistance, capability of degrading nitrite, tryptamine, tyramine, spermidine and spermine, no generation of biogenic amine, no degradation of pectin, antibacterial activity on pathogenic bacteria, oxidation resistance of extracellular metabolites, and no pollution to the environment. According to the present invention, the new strain has advantages of high yield, key volatile substance production and non-volatile substance production, and the fermented food prepared by using the new strain has characteristics of low salt content, rich lactic acid bacteria content, nutrition, health and unique flavor; a food fermentation process developed by utilizing the new strain disclosed by the invention is high in fermentation efficiency, short in production period and high in application value.

The application discloses a compound Cl-BODIPY used as a fluorescent probe for rapid nondestructive detection of food freshness, and also establishes a corresponding preparation method and detection method. The probe produces a color and fluorescent dual optical signal change through specific interaction of a recognition group with volatile biogenic amines, and provides a simple and convenient detection method for rapid detection of volatile biogenic amines. The fluorescent probe is simple to prepare, low in cost, and has the characteristics of high sensitivity, high selectivity and rapid response, can in-situ rapidly detect volatile biogenic amines produced by fish and meat food spoilage, and judges the freshness (biogenic amine content) of fish and meat through colorimetric and fluorescent dual visual signal changes, thereby providing an effective means for rapid nondestructive detection of food freshness, having great application prospect and great popularization significance.

Disease modification of specific neurodevelopmental disorders is demonstrated for preschool children through modulation of specific biogenic amine neurotransmitters and y-aminobutyric acid (GABA) / glutamine. In specific, cumulative reductions in maladaptive behaviors that interfere with activities of daily living for preschool populations are achieved through staged, long term, treatment. Additionally, a prophylaxis for certain neurodegenerative disorders is presented. Provided herein are compositions comprising digestive enzyme preparations and methods of using same for treating an Autism Spectrum Disorder (ASD).

The invention discloses application of AtTyrDC in catalyzing aromatic amino acid to synthesize biogenic amine. A TyrDC gene (AtTyrDC) is screened and identified in an arabidopsis thaliana genome by utilizing a bioinformatics method, and the TyrDC gene is further verified to have tryptophan decarboxylase activity through in-vitro enzyme activity analysis. In addition, the invention also provides an AtTyrDC mutant, and compared with the sequence shown in SEQ ID NO.1, the mutant comprises the substitution of Q369L. The invention finds that a Q369L substituted AtTyrDC mutant obviously enhances the decarboxylation activity on a substrate aromatic amino acid. The invention lays a foundation for improving metabolic pathways to enhance the production of biogenic amines in crops, and has wide application prospects.

The invention discloses lactococcus lactis subsp.hordei JYZ4 with biogenic amine degradation capacity, and belongs to the technical field of microorganisms. The preservation number of the lactococcus lactis subsp. Houdei JYZ4 of the strain is CGMCC (China General Microbiological Culture Collection Center) No.17892, and the lactococcus lactis subsp. Houdei JYZ4 shows good safety in the aspects of hemolysis, drug resistance, virulence genes and the like. In a culture medium system, the strain can simultaneously degrade eight biogenic amines (tryptamine, beta-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine), the highest tyramine degradation rate (49.74% + / -1.89%) is achieved, and the degradation rate is remarkably higher than that of a control strain. In a high biogenic amine pickle fermentation system, the degradation rates of eight biogenic amines are obviously higher than those of a control strain within a fermentation period of 1-7 days. The lactococcus lactis subsp.hordei JYZ4 disclosed by the invention can effectively reduce the biogenic amine content of a fermented product and improve the safety of fermented food.

The invention discloses a method for reducing the content of biogenic amines in meat products through composite vacuum rapid pickling, and belongs to the technical field of food processing. According to the method disclosed by the invention, the pork tenderloin, the chicken breast and the beef tendon are pickled by adopting the pickling liquid prepared by compounding the anthocyanin and the lipopeptide and assisting ultrasonic and vacuum modes, so that the pickling time of the meat product is shortened, the quality of the meat product is improved, the generation of biogenic amine is also inhibited, histamine and tyramine are not detected in the pork tenderloin pickled by the method, and the pork tenderloin is free of toxic and side effects. The content of cadaverine and spermidine is remarkably reduced, and the product is uniform in meat quality, excellent in flavor and high in safety. The method provides a theoretical basis for controlling the safety of meat products and optimizing the processing technology while improving the meat pickling efficiency, and has a key guiding significance for developing low-salt and low-biogenic-amine meat products.

The present disclosure relates to a formulation for iron chelation therapy comprising: a water-soluble polyphenol-biogenic amine complex comprising of a polyphenol and an amine, and a 0.9% of sodium chloride; optionally one or more additive, wherein water-soluble polyphenol-biogenic amine complex effectively chelates the excess iron and enable its elimination. The present disclosure also provides a method of preparation of a formulation for iron chelation therapy comprising: a) adding 0.005 to 5% w / w of a polyphenol and 0.005 to 5% w / w of an amine followed by adjusting to pH-7 by adding HCl / NaHCO3 to form a mixture; and b) mixing the mixture of step a) with suitable proportion in 0.9% sodium chloride and optionally one or more additive to form a formulation for iron chelation therapy, wherein water-soluble polyphenol-biogenic amine complex effectively chelates the excess iron and enable its elimination.

The invention discloses an application of a reagent containing multi-copper oxidase in reducing the biogenic amine content of a fermented meat product, recombinant multi-copper oxidase is obtained through a genetic engineering technology, and the multi-copper oxidase is developed into an enzyme reagent for degrading biogenic amine in Sichuan sausages. Results show that the addition of the reagent containing the multi-copper oxidase and the diamine oxidase has no influence on the eating quality and the sensory quality of the Sichuan sausages, in terms of the biogenic amine content, after the reagent is added, the degradation rates of histamine, tyramine, cadaverine and putrescine are at least 49.63%, 38.51%, 38.26% and 35.40%, and the degradation rates of the reagent containing the multi-copper oxidase and the diamine oxidase are at least 49.63%, 38.51%, 38.26% and 35.40%. The reagent provided by the invention has a good degradation effect on four main biogenic amines in Sichuan sausages.

This invention relates to a method for evaluating the spectra of biological material of animal, plant, or mixture thereof, the method comprising the steps of: a) detecting spectrometers in a network formed by at least one spectrometer and an input / output device; b) requesting the respective status of each spectrometer in the network of step a), and displaying the detected spectrometers and their status on the input / output device, wherein the status reflects whether the spectrometer is available for recording spectra; c) receiving a selection from spectrometers available for recording spectra on the input / output device; d) recording the spectrum of the sample material of animal, plant, or mixture thereof on the spectrometer selected in step c); e) predicting at least one parameter from the spectrum of step d) using at least one calibration function and / or calibration plot suitable for predicting the value of the parameter. The values ​​of the parameters, wherein the at least one parameter is selected from the content of at least one amino acid, crude protein content, ammonia content, content of all amino acids containing ammonia, content of all amino acids not containing ammonia, crude fat content, dry matter content, crude ash content, energy content, content of at least one biogenic amine, content of at least one anti-nutritional factor, content of at least one sugar, starch content, crude fiber content, neutral detergent fiber content, acid detergent fiber content, total phosphorus content, phytic acid phosphorus content, reactive lysine content, total lysine content, ratio of reactive lysine content to total lysine content, protein dispersibility index, protein solubility, trypsin inhibitor activity, urease activity, and processing condition index (PCI); and f) displaying the predicted results of step e) on the input / output device.

The invention relates to a fermentation method for reducing biogenic amine in fish sauce, which comprises the following steps: 1) taking fresh fish meat, adding 30-50% of sterile water, 18% of salt, 0.5-2% of neutral protease with activity of 2 * 10 < 4 > U / g and 0.03% of sodium pyrophosphate by mass, uniformly mixing and crushing, and bottling; (2) fermenting at the constant temperature of 35 DEG C for 72 days, determining the oxidation-reduction potential in the fermentation process of the fish sauce by using an oxidation-reduction potential depolarization method automatic determinator in the fermentation process, adding a proper amount of hydrogen peroxide solution with the mass fraction of 0.5% every three days, controlling the oxidation-reduction potential of the fish sauce fermentation liquid to be 300-340mV until the fermentation end point, and during the fermentation period, adding a proper amount of water into the fish sauce fermentation liquid, water is supplemented once every three days. The fermentation method is simple to operate and easy to implement, the content of biogenic amine, especially tyramine, of the fish sauce is remarkably reduced, and the quality of the obtained fish sauce is good.

The invention discloses a preparation method for reducing biogenic amine in red intestines by using lactobacillus plantarum diamine oxidase. The preparation method comprises the following steps: culturing lactobacillus plantarum SH7, extracting and purifying the diamine oxidase and applying the diamine oxidase to the red intestines. High-activity enzyme powder is obtained through ammonium sulfate salting-out and anion exchange chromatography and added into the sausage raw material, biogenic amine is effectively degraded, the safety of the sausage is improved, and the product quality is not affected. The method is simple in process and suitable for industrial production, and provides a new way for biogenic amine control of fermented meat products.

The application discloses a kind of biogenic amine response fluorescent color-changing hydrogel microneedle patch, and preparation method and application thereof, belong to food detection technical field.The method for preparing biogenic amine response fluorescent color-changing microneedle patch of the application includes the following steps: (1) two fluorescent dyes are dissolved in dimethyl sulfoxide and ultrapure water respectively, after mixing according to proportion, obtain solution A that responds to biogenic amine;(2) solution A is added to hydrogel material, horseradish peroxidase (HRP), hydrogen peroxide (H2O2) to prepare mixed fluorescent hydrogel solution, which is dropped on the mold containing patch needle tip prepared;By drying, fluorescent color-changing hydrogel microneedle patch is prepared.The microneedle patch prepared by the method of the application is applied to rapid biogenic amine response, can respond to color in the storage process of fresh meat produced by biogenic amine, suitable for consumer on-site detection of fresh meat, so as to purchase healthy, high-quality food, with wide application prospect.

This invention discloses a method for simultaneously and accurately determining the content of multiple biogenic amines in baijiu (Chinese liquor). Based on gas chromatography-triple quadrupole mass spectrometry (GC-MS), it detects multiple biogenic amines in baijiu, including nicotinamide, β-phenylethylamine, guanidine, and putrescine. In the pretreatment process, 1,7-diaminoheptane is added as an internal standard, and a mixture of N,O-bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane is used as a derivatization reagent, effectively simplifying the pretreatment process. By controlling the conditions of GC and MS, accurate, rapid, and highly sensitive determination of multiple biogenic amines in baijiu is achieved. In this invention, baijiu samples do not require extraction with organic solvents or pH adjustment; the pretreatment operation is extremely simple, and the sensitivity and quantitative accuracy of biogenic amines are high, with good stability and reliability. This effectively improves the problem of complex pretreatment operations and poor detection accuracy in the quantitative determination of biogenic amines in baijiu.

The invention provides a fluorescence detection method for detecting histamine, hydrogel and a preparation method and application thereof, and belongs to the technical field of biogenic amine detection. In particular to a fluorescence detection method for detecting histamine, which mainly comprises the following steps: mixing a copper nano-cluster solution, a carbon dot solution and histamine solutions with different concentrations, then fixing the volume, detecting the fluorescence emission spectrum of each system under the excitation wavelength of 365 nm, and calculating the fluorescence intensity of each system by taking the ratio F437 / F605 of the fluorescence intensity at the wavelength of 437 nm and 605 nm as the ordinate. Making a standard curve by taking the concentration of histamine in each system as an abscissa, and detecting the concentration of histamine in a sample to be detected; the invention also provides a hydrogel which is used for detecting histamine. The fluorescence detection method provided by the invention is used for detecting histamine, the histamine detection selectivity is strong, the specificity is good, and the hydrogel prepared by the invention realizes visual rapid detection of histamine.

The invention discloses a method for preparing germinated wheat by using physical field assisted salt stress, which optimizes the component change in the wheat germination process through the synergistic effect of ultrasound, salt stress and heat treatment. Researches show that the process can significantly improve the functionality and trophism of the germinated wheat flour: functional components (resistant starch, GABA and total phenols) are accumulated first and then stabilized, the peak value of the resistant starch reaches 5.32%, the peak values of the GABA and the total phenols respectively reach 5.29 mg / g and 2.53 mg / g, and the degradation in the later stage is slow; protein (13.74%) and dietary fiber (4.37%) are continuously accumulated, and the peak value of the germination rate reaches 92%-93%. Meanwhile, the process can effectively control the content of harmful substances, and DON, aflatoxin and biogenic amine are finally reduced to 75.82 mu g / kg, 0.58-0.65 mu g / kg and 2.04-2.25 mg / kg respectively, which are far superior to those of single stress or natural germination treatment. Through cooperation of multiple processes, synchronous improvement of functionality, nutrition and safety of the germinated wheat flour is realized, and the germinated wheat flour has relatively high industrial application value.