This invention discloses a
visual detection method and application of biogenic amines based on a
carbon dot /
quantum dot ratio fluorescent probe, belonging to the field of
food safety detection technology. Addressing the problems of existing
biogenic amine detection methods, such as complex operation, difficulty in rapid on-site screening, and susceptibility to interference from single fluorescent signals, this invention prepares a ratio fluorescent probe based on
graphene carbon dots (G-CDs) and near-
infrared quantum dots (QD700). This probe requires no
chemical modification and achieves a synergistic response of G-CDs
fluorescence enhancement and QD700
fluorescence quenching through the Spe-induced aggregation effect, achieving a
detection limit of 0.0097 μM and a
response time faster than 2 seconds. Combined with the DEIM intelligent model, it can accurately distinguish
spermine,
putrescine,
cadaverine,
histamine, and
tyramine. Integrated into a paper-based smartphone platform, it enables
visual identification of
shrimp freshness (red for fresh, green for spoiled), showing a high correlation with the TVB-N standard method (R²=0.95), suitable for rapid on-
site monitoring.