102 results about "Biogenic amine formation" patented technology

The invention discloses a polyimidazolone compound as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a bilateral alkyne ester compound, amidine hydrochloride, a catalyst and a desalting agent in an organic solvent, and carrying out polymerization reaction to obtain the polyimidazolone compound. The polyimidazolone compound with the five-membered ring is selectively generated, and the method has the advantages that monomers are easy to obtain, operation is easy, and by-products are low in toxicity. The polyimidazolone compound prepared by the invention has better machinability and has potential application value in the aspects of metal ion detection and biogenic amine detection.

The invention relates to the technical field of biogenic amine detection, in particular to a dicyanoethylene-based fluorescent material as well as a preparation method and application thereof. After the biogenic amine is in contact with the glass surface fluorescent probe, due to the nucleophilic characteristic of the biogenic amine, the biogenic amine can be subjected to nucleophilic substitution reaction with a probe molecule dicyanovinyl group to generate an imine chemical structure. As the electron-withdrawing capability of dicyanoethylene is stronger than that of imine, the intramolecular charge transfer effect of probe molecules is greatly reduced, so that blue shift of absorption and luminescence properties of the probe molecules is caused, and after the fluorescent probe is loaded on the surface of glass, the fluorescence intensity of the fluorescent probe is improved. The formed dicyanoethylene-based fluorescent material provides a solution for the technical problem that the existing biogenic amine detection mode is inconvenient.

The invention relates to a porphyrin metal organic framework composite material, a preparation method and a biogenic amine SERS (Surface Enhanced Raman Scattering) array sensor. The preparation method of the composite material comprises the steps that a copper precursor solution is added into a surfactant, heating reaction is conducted, a reducing agent is added, heating reaction is conducted, and copper nanoparticles are obtained; the preparation method comprises the following steps: adding a transition metal salt and a surfactant into an organic solvent, adding a porphyrin ligand, carrying out a solvothermal reaction, carrying out centrifugal separation, washing and drying to obtain a porphyrin metal organic framework material, and dispersing the porphyrin metal organic framework material into the organic solvent to obtain a dispersion liquid A; and dispersing copper nanoparticles in the dispersion liquid A, carrying out a second heating reaction, and carrying out centrifugal separation, washing and drying to obtain the porphyrin metal organic framework composite material. The invention also provides the porphyrin metal organic framework composite material prepared by the preparation method, and the application of the porphyrin metal organic framework composite material as a biogenic amine SERS array sensor. The invention solves the problem that the accuracy of the detection result is influenced because the traditional biogenic amine detection method is difficult to realize field detection.

The present invention relates to functional foods and dietary supplements comprising a specific diamine oxidase (DAO) enzyme derived from Glutamicibacter halophytocola, uses of said enzyme and respective methods for the production of biogenic amine-depleted products, uses of said enzyme as a biosensor for the analysis of one or more biogenic amine(s), as well as said enzyme for use in medicine, in particular for use in the prevention or treatment of a condition or disease that is associated with increased levels of biogenic amines.

The invention discloses a lactobacillus paracasei CN23, a preparation method of a microbial agent and a preparation method of fermented bean curd, and relates to the technical field of microorganisms. The invention relates to a lactobacillus paracasei CN23, which is preserved in the China General Microbiological Culture Collection Center on September 2, 2024, and the preservation number is CGMCC NO. 31794. The lactobacillus paracasei CN23 is named as the lactobacillus paracasei CN23. The fermented bean curd prepared by fermenting the acid water with the microbial agent containing the casei paracasei CN23 is higher in total acid content and amino acid nitrogen content, richer and more complex in flavor and rich in layering sense, the total biogenic amine content and the white dot ratio are remarkably reduced, and the fermented bean curd has the advantages of being good in taste and flavor, rich in nutrition, good in taste and rich in flavor. The fermented bean curd has the characteristics of no food safety risk, no preservative and coagulator, and significantly improves the quality of the fermented bean curd.

The application discloses a fluorescent label for nondestructive visual monitoring of freshness of fish and meat food and a preparation method thereof. The fluorescent label is characterized in that a key fluorescent substance JDCN specifically interacts with volatile biogenic amines generated in the meat spoilage process, color and fluorescence double optical signal changes occur, the biogenic amine content is judged according to the visual optical characteristics, and thus the freshness of the fish and meat food is monitored. The application has the characteristics of simple preparation, low cost, high sensitivity, high selectivity, fast visual response, and the like, can in-situ rapidly detect the volatile biogenic amines generated in the spoilage of the fish and meat food, provides a simple, convenient and effective means for the rapid visual detection of the freshness of the fish and meat food, is expected to become a preferred method for rapid detection of the content of the volatile biogenic amines in the fish and meat food, provides a powerful technical guarantee for self-monitoring of food manufacturers and market supervision, has great application prospect, and is of great popularization significance.

The disclosure provides microbial composition and application in reducing biogenic amines, and belongs to the technical field of food processing and manufacturing. The disclosure provides a construction method for an inoculated biogenic amine degrading lactic acid bacteria flora and an application in reducing biogenic amines in cyclic rice steeping and persistently inhibiting invasion of amine producing microorganisms. In the disclosure, a lactic acid bacteria ternary interacting flora that efficiently inhibits invasion of the amine producing microorganisms is constructed. In a rice steeping process in preparation of a brewed product, the lactic acid bacteria ternary interacting flora is inoculated for rice steeping, and a cyclic rice steeping process is improved. The result shows that inoculation of the lactic acid bacteria ternary interacting flora and utilization of the improved rice steeping process may effectively reduce the content of biogenic amines in rice steeping water and completely inhibit invasion of the amine producing microorganisms to realize zero growth of the biogenic amines, thereby creating conditions for the cyclic rice steeping process in mass production in a plant.

The invention relates to the technical field of fermentation, in particular to a hazelnut meal fermented product as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: inoculating Leuconostoc mesenteroides into fermentation liquor containing hazelnut meal, and fermenting, so as to obtain a hazelnut meal fermentation product; the Leuconostoc mesenteroides is preserved in the China Center for Type Culture Collection, and the preservation number of the Leuconostoc mesenteroides is CCTCC (China Center for Type Culture Collection) NO: M2024452. According to the invention, specific leuconostoc mesenteroides is utilized, tyrosine, phenylalanine and the like in metabolites obtained by fermentation are precursors for synthesizing neurotransmitters, and in addition, some biogenic amines obtained by fermentation have a synergistic refreshing function. Meanwhile, in the fermentation process, protein can be decomposed into amino acid and polypeptide, a Maillard reaction is carried out to generate pyrazine substances with flavors of baking, nuts and the like, and the flavor is further enriched by the flavors generated by fermentation of other protein.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention discloses lactobacillus plantarum for overexpressing a luxS gene, a microbial preparation and application of the lactobacillus plantarum, and belongs to the technical field of microorganisms. In order to solve the technical problem that excessive biogenic amines are accumulated in a fermented meat product in the prior art, the invention provides lactobacillus plantarum for overexpressing a luxS gene and a microbial preparation containing the lactobacillus plantarum. The lactobacillus plantarum for overexpressing the luxS gene, provided by the invention, has the capability of highly producing signal molecules AI-2 and has the capability of remarkably improving the activities of diamine oxidase and polyketide oxidase; the lactobacillus plantarum overexpressing the luxS gene and the microbial preparation containing the lactobacillus plantarum can be used as a leavening agent for reducing biogenic amine to be applied to production of fermented meat products, the content of biogenic amine in the air-dried sausages can be effectively reduced, and the quality and safety of the air-dried sausages are improved.

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.

The present invention belongs to the technical field of food biogenic amine detection, and discloses an organic field effect transistor gas sensor, an odor measurement circuit and a method. According to the present invention, the organic field effect transistor and the odor detection circuit sense food biogenic amine, causing changes in the internal characteristics of the organic field effect transistor, which in turn leads to changes in the circuit voltage signal. The voltage gain of the feedback circuit is adjusted according to the voltage signal displayed on the oscilloscope, so that the voltage displayed on the oscilloscope is equal to the source voltage, realizing the adjustment of the voltage gain by the mobile terminal. Based on the relationship between the source voltage and the voltage gain, the changes in the charge carrier mobility and the threshold voltage are determined. Thus, the detection result of food biogenic amine is obtained according to the changes in the charge carrier mobility or the threshold voltage, achieving the purpose of rapid on-site detection.

The invention relates to the technical field of wine brewing, in particular to hansenula polymorpha cloud-268, application of the hansenula polymorpha cloud, a fermentation method of dry red wine and a microbial agent. The hansenula polymorpha var. Uvarum cloud-268 is preserved in the China Center for Type Culture Collection on December 13, 2013, and the preservation number of the hansenula polymorpha var. Uvarum cloud-268 is CCTCC NO: M2013658. According to the invention, non-saccharomyces cerevisiae YUN-268 and saccharomyces cerevisiae are preferably selected for synergistic fermentation, and the stability of a fermentation system is maintained by using the fermentation biological protection effect of the non-saccharomyces cerevisiae. Accumulation of potential harmful organism amine is remarkably reduced, generation of aroma substances of the grape wine is promoted, and the aroma of the obtained grape wine is richer. The method provides a feasible and efficient technical scheme for green brewing of low-sulfur and high-quality wine, has the advantages of greenness, safety and high quality, and provides a technical path capable of being popularized for industrial production of the low-sulfur dry red wine.

The present invention provides for methods of treating biogenic amine related conditions. Further provided are methods of detecting biogenic amine related conditions, and selection of treatment for patients in need thereof.

The invention discloses pediococcus pentosaceus Ppe-YC39 capable of enhancing the nutritional activity and flavor of fish tea and inhibiting formation of biogenic amine and application of the pediococcus pentosaceus Ppe-YC39. Wherein the pediococcus pentosaceus Ppe-YC39 is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the pediococcus pentosaceus Ppe-YC39 is GDMCC No: 66920. The pediococcus pentosaceus Ppe-YC39 disclosed by the invention has a probiotic attribute equivalent to or even better than that of a commercial strain lactobacillus plantarum LGG, and when the pediococcus pentosaceus Ppe-YC39 is applied to fish tea fermentation, the content of amino acid and fatty acid in the fermented fish tea can be remarkably increased, and the content of biogenic amine is remarkably reduced.

The invention discloses lactobacillus plantarum XHQ-007 capable of degrading biogenic amine and application of the lactobacillus plantarum XHQ-007. The lactobacillus plantarum XHQ-007 is preserved in Guangdong Microbial Culture Collection Center on March 14, 2025, and the preservation number is GDMCC No: 66016. The lactobacillus plantarum XHQ-007 can be used for degrading biogenic amine. The strain has no biogenic amine synthesis capability, has a biogenic amine degradation function, can effectively reduce the content of biogenic amine in fermented food, has a tyramine degradation rate of 79.94%, a histamine degradation rate of 59.01% and a tryptamine degradation rate of 75.23%, can effectively control the content of biogenic amine in the production process of fermented food such as fruit wine and the like, and has a good application prospect. And a healthy and safe fermented product is provided for consumers.

The application discloses a kind of meat preservation and freshness visual double-effect nano intelligent indicating film and preparation method thereof, belong to food packaging storage and detection technical field.The preparation method of the intelligent indicating film includes the following steps: (1) poly-3-hydroxybutyric acid-co-oxyvalerate (PHBV) and ferulic acid are dissolved in chloroform solution;(2) polyvinyl alcohol (PVA), konjac glucomannan (KGM), alizarin (AL) are mixed, and polyvinyl alcohol-konjac glucomannan-alizarin mixed solution is obtained by removing bubble;(3) PH-F solution is dried by electrospinning technology, and PVA-KGM-AL@PH-F double-layer indicating film is obtained.The application prepares meat freshness indicating film with efficient antibacterial performance and high sensitivity color response performance by regulating the pH of film-forming solution.In the intelligent indicating film, alizarin responds to meat spoilage biogenic amine, and the color of indicating film gradually changes from cream (slightly yellow) to light pink or even deep purple;By comparing the intelligent indicating film after reflection with colorimetric card, the freshness of meat can be judged.

The invention discloses a brewing method of yellow rice wine, which adopts lactobacillus sake L.48 with the preservation number of CCTCC NO: M 20221306, the strain deletes histidine decarboxylase gene, the CRISPR (clustered regularly interspaced short palindromic repeats) repetition number is 39, and biogenic amine can be obviously reduced. According to the process, rice cleaned with warm water is taken as a raw material, lactobacillus sake L.48 is inoculated for co-fermentation with yeast and distiller's yeast, primary fermentation is performed at 25-32 DEG C, secondary fermentation is performed at 18-25 DEG C, and the process is completed within 23 days. Compared with a traditional rice soaking method, the production period is shortened, the biogenic amine content in the product is reduced by more than or equal to 60%, and the ester aroma is improved by 2-3 times.

The invention discloses a liquid mass spectrometry detection method for biogenic amines in bee tissues, and relates to the technical field of compound detection. The detection method comprises the following steps: dissecting to obtain a bee brain tissue sample (with a single eye and a compound eye removed), putting the sample into a centrifugal tube, adding an extracting solution, namely a hydrochloric acid aqueous solution, crushing the tissue crushed sample by an electric grinder, carrying out ultrasonic treatment on precipitated protein, transferring supernate into a filter type sample bottle, filtering, and carrying out liquid chromatography-mass spectrometry determination. The invention establishes a method for simultaneously and rapidly detecting four biogenic amines in a bee tissue sample by HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry), the method can simultaneously analyze four chemical substances (biogenic amines) within 8 minutes, the detection time is short, the linear relation is better, the method recovery rate is high, the stability is good, and the analysis requirement is met.

The invention relates to the technical field of biology, in particular to lactic acid bacteria with a preservative function and application of the lactic acid bacteria, the lactic acid bacteria are Lactobacillus plantarum GXSS-0004, the strain preservation number of the lactic acid bacteria is GDMCC No.65451, the lactic acid bacteria are preserved in Guangdong Microbiological Culture Collection Center on November 8, 2024, and the preservation unit address is Institute of Microbiology, Guangdong Academy of Sciences, Floor 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The Lactobacillus plantarum GXSS-0004 disclosed by the invention can be used for fermenting and producing acid by utilizing saccharides under a culture medium and a growth condition, so that the growth and reproduction of the Lactobacillus plantarum GXSS-0004 are promoted, and the Lactobacillus plantarum GXSS-0004 has the characteristics of antibacterial activity and drug resistance, also has the capability of degrading nitrite and generating biogenic amine, and can play a role in corrosion prevention in preparation of pickled leaf mustard, raphanic acid, pickled Chinese cabbage and pickled pawpaw.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Infantis, a probiotic preparation and application of the probiotic preparation in products for reducing blood sugar and blood lipid. The bifidobacterium longum subsp. Infantis provided by the invention is bifidobacterium longum subsp. Infantis A32-03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32811. The strain does not generate hemolysin, does not have nitrate reductase activity, does not generate any harmful metabolites such as biogenic amines and indoles, is sensitive to various antibiotics, and is good in biological safety. Particularly, the probiotic preparation obtained by fermenting the bifidobacterium longum subsp. Infantis A32-03 in a culture medium containing the auricularia auricula polysaccharide has a prominent inhibition effect on alpha-amylase, alpha-glucosidase and lipase, has an excellent degradation capability on cholesterol, has a good blood sugar and blood fat regulation effect, and can be used for preparing the probiotic preparation. The method has good popularization value in oral administration of medicines for reducing blood sugar and blood fat or health-care food for regulating blood sugar and blood fat.

A low-temperature cured bio-based high-toughness epoxy asphalt material relates to the technical field of preparation of road engineering materials, and is prepared from the following raw materials in parts by mass: bisphenol A type epoxy resin, bio-based epoxy resin, a biogenic amine curing agent, an epoxy diluent, a catalyst and matrix asphalt. The asphalt comprises the following components in parts by weight: 15-30 parts of bisphenol A type epoxy resin, 5-18 parts of bio-based epoxy resin, 10-20 parts of a biogenic amine curing agent, 1-5 parts of an epoxy diluent, 0.5-3 parts of a catalyst and 35-55 parts of matrix asphalt. The preparation method of the low-temperature cured bio-based high-toughness epoxy asphalt material comprises four steps. The preparation raw materials mainly come from renewable biomass resources, the production does not depend on non-renewable petroleum resources, and the prepared finished product has good degradability, reduces the risk of environmental pollution, has certain chemical stability and physical properties, and is suitable for industrial production. The pavement performance such as high-temperature rut resistance, low-temperature crack resistance and water damage resistance of the road asphalt mixture can be improved, and the overall quality of the road mixture can be improved.

This invention provides a rare earth metal-based ternary composite antibacterial agent and its preparation method. The antibacterial agent is prepared from raw materials including GL-22, Eu-containing polyoxometalate clusters, and Spm. The rare earth metal-based ternary composite antibacterial agent of this invention, after fractional compounding of Eu-containing polyoxometalate clusters with peptides and biogenic amines, exhibits highly efficient antibacterial function against Escherichia coli and Staphylococcus aureus. The ternary assembly EuW... 10 The fluorescence emission of / GL-22 / Spm is enhanced by 30-40 times, and it is effective against Escherichia coli (E. coli). E. coli The antibacterial properties of the drug have been increased from 40%~30% to 90%~95%, and its efficacy against bacteria has been improved. E. coli The inhibition rate of biofilm (BME) formation was 21.8%.

This invention discloses a method for preparing a highly antioxidant, color-changing, intelligent active food preservation film containing bilberry anthocyanins, belonging to the field of food preservation technology. An ACNs-CHC / CMC-WPI nanocomposite is constructed through the encapsulation of chitosan hydrochloride (CHC), carboxymethyl chitosan (CMC), and whey protein isolate (WPI). Using corn starch, chitosan, and 30 wt% glycerol as a base material, the anthocyanin-loaded nanocomposite is introduced as a functional component and formed on the surface of an acrylic sheet via solution casting. This food preservation film exhibits excellent mechanical properties and effectively inhibits spoilage and the proliferation of pathogenic bacteria in high-protein aquatic foods (such as white shrimp), reduces the formation rate of biogenic amines, and significantly slows down the deterioration process of food quality. Furthermore, the surface color of the film changes with the pH value of the target meat, providing a visual monitoring method for food freshness and can also be used for rapid detection of food freshness.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The invention discloses application of a multi-copper oxidase mutant in biogenic amine degradation, and belongs to the technical field of food biology. The amino acid sequence of the multi-copper oxidase mutant is shown as SEQ ID NO.1. The application is to degrade biogenic amines in aquatic products, and the biogenic amines comprise tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine. The multi-copper oxidase mutant disclosed by the invention not only can effectively reduce the content of biogenic amines in aquatic products and reduce the generation of reaction byproducts, but also can maintain the freshness and nutritional value of the aquatic products, and has remarkable application value in the fields of aquatic product processing and food safety.

The invention relates to the field of microorganisms, and provides a plant lactobacillus FVPGZP2101 and application thereof, the plant lactobacillus FVPGZP2101 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32871. The invention also provides a preparation method of the plant lactobacillus FVPGZP2101. The new strain of the plant lactobacillus obtained by screening has the advantages of strong growth ability, high acid production efficiency, strong acid resistance, capability of degrading nitrite, tryptamine, tyramine, spermidine and spermine, no generation of biogenic amine, no degradation of pectin, antibacterial activity on pathogenic bacteria, oxidation resistance of extracellular metabolites, and no pollution to the environment. According to the present invention, the new strain has advantages of high yield, key volatile substance production and non-volatile substance production, and the fermented food prepared by using the new strain has characteristics of low salt content, rich lactic acid bacteria content, nutrition, health and unique flavor; a food fermentation process developed by utilizing the new strain disclosed by the invention is high in fermentation efficiency, short in production period and high in application value.

The invention belongs to the field of cell culture, and discloses a mesenchymal stem cell culture medium with limited chemical components, which can be used for cell culture and exosome harvesting. The culture medium consists of a basic culture medium and an additive component, wherein the additive component is selected from one or more of a growth factor substance, a binding protein substance, an adherent factor substance, a cell factor substance, a hormone substance, a trace element-containing substance, a biogenic amine substance, an amino acid substance and a nucleoside substance. The culture medium is free of serum, human source and animal source components, the contained components are completely known, the motility rate, proliferation and cell quality of the mesenchymal stem cells in cell culture can be remarkably improved, and therefore the technical problem that in the prior art, an existing mesenchymal stem cell limited component culture medium is poor in culture effect is solved.

The present invention discloses a ratiometric near-infrared fluorescent probe, its synthesis method and applications, belonging to the field of organic small molecule ratiometric fluorescent probes. The molecular formula of the near-infrared fluorescent probe is C 32 H 31 N2O3 + , labeled as FS-B1. The maximum absorption wavelength of the near-infrared fluorescent probe in acetonitrile and PBS (30% ACN) is 640 nm, the fluorescence spectrum wavelength is 765 nm, the fluorescence performance is stable, and the response time is fast. In acetonitrile, as the concentration of organic amine increases, and in PBS (30% ACN), as the concentration of NaHSO3 increases, the emission peak of the near-infrared fluorescent probe at 765 nm gradually decreases, the emission peak at 483 nm gradually increases, the absorption peak at 640 nm gradually decreases, the absorption peak at 400 nm gradually increases, and the color of the solution changes significantly and can be distinguished by the naked eye. The near-infrared fluorescent probe realizes the detection of trace amounts of organic amines and NaHSO3 with high sensitivity, and can efficiently and selectively detect trace amounts of biogenic amines produced during food spoilage, showing good application prospects in the field of food safety.