36 results about "Copper oxidase" patented technology

The invention discloses a multi-copper oxidase mutant with improved salt tolerance. The present invention mutates the 317th threonine into asparagine, the 386th leucine into tyrosine, and the 427th serine into glutamic acid in the wild-type multi-copper oxidase WT by site-directed mutation, forming Mutant T317N‑L386Y‑S427E. Compared with WT, T317N‑L386Y‑S427E had improved tolerance to 6%, 9%, 12%, 15%, and 18% NaCl (W / V).

The present invention relates to an amine oxidase ACAO, and the amino acid sequence of the amine oxidase ACAO is SEQ ID NO.1. The amine oxidase ACAO of the present invention has a degradation rate of 100% to HFB1; the amine oxidase ACAO of the present invention has excellent properties, and the enzyme can be applied to industries such as agriculture, feed and food to reduce the harm of fumonisin FB1 to animal and human health .

The invention discloses a maize multi-copper oxidase coding gene ZmDEK559-2. The CDS sequence of the gene is shown in SEQ ID No.1, and the encoded protein sequence is shown in SEQ ID No.2. The invention also discloses the application of the maize polycopper oxidase coding gene ZmDEK559‑2 or the plant expression vector containing the gene in breeding for improving crop tolerance to drought or high-salt adversity stress. Experiments have confirmed that the drought resistance and salt tolerance of maize overexpressing the ZmDEK559‑2 gene have been significantly improved compared with the untransformed wild-type control, and it can maintain relatively good growth when encountering adversity stress such as drought and high salinity, thereby promoting crop growth. The increase in yield indicates that the invention has broad application prospects in crop stress resistance genetic breeding improvement.

The invention discloses a method for enzymatically producing 4-hydroxyphenylacetaldehyde, which belongs to the field of biotechnology. The method comprises cloning a highly active primary amine oxidase gene, connecting an expression vector and transforming Escherichia coli competent cells, and constructing a primary amine oxidase gene. Amine oxidase engineering bacteria, and then use the fermented primary amine oxidase to catalyze the oxidation of tyramine to produce 4-hydroxyphenylacetaldehyde. The invention adopts an enzymatic method to produce 4-hydroxyphenylacetaldehyde, the reaction conditions are mild, the environment is friendly, and the specificity of the biological enzyme catalytic reaction is strong. The two-phase catalytic system is used to reduce the inhibitory effect of the product on the enzyme, the reaction yield is high, and the catalysis is compatible with the enzyme. Separation and coupling are beneficial to product extraction, and at the same time, the raw materials used in the production method of the present invention are cheap and easy to obtain, and the production process is simple and easy, and the production cost is low.

The invention discloses a monoamine oxidase A inhibitor, which is obtained by using cheap ruthenium carbon as a catalyst to catalyze the synthesis of thioether at room temperature, specifically: using the compound represented by formula (III) as the reaction raw material, Ru / C as catalyst, NaIO 4 is an oxidant, water is a solvent, and the reaction is carried out at room temperature. After the reaction is completed, the reaction liquid is post-treated to obtain the sulfone compound shown in formula (IV). The method of the invention uses water as a solvent, oxidizes cheap ruthenium carbon with sodium periodate to generate ruthenium tetroxide, and further oxidizes the sulfide compound to synthesize the target product, and the preparation method is more efficient, quick, environmentally friendly and mild. The prepared monoamine oxidase A inhibitor has good monoamine oxidase A inhibitory activity, can be used to prepare a drug for inhibiting the activity of monoamine oxidase A, and has broad application prospects in drug development, in order to provide a more effective way for the treatment of related diseases.