The invention provides a preparation for degrading printing and dyeing pollution in a natural fabric. The preparation is prepared from, by weight, 10-20 parts of a citrus extract, 5-15 parts of a bacterium degradation preparation, 3-10 parts of maltose, 2-5 parts of betaine, 1-3 parts of baking soda, 2-5 parts of glucose oxidase, 1-3 parts of pectinase, 1-2 parts of cellulase, 2-5 parts of a metalchelating agent, 1-2 parts of tween-80, 1-2 parts of span-80 and 100-120 parts of deionized water. The glucose oxidase is separated from penicillium notatum, the pectinase is separated from aspergillus niger, and the cellulase is separated from basophilic bacteria. The preparation method of the preparation is simple, the raw material sources are wide, the cost is low, and the preparation has a good degradation effect on the printing and dyeing pollution of the natural fabric, is free of pollution, toxicity and harm, has fragrance and is popular among mass consumers.