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Bacterial strain capable of degrading mold toxin and formulation preparation method thereof

A technology for mycotoxins and preparations, which is applied in the field of preparation of strains capable of degrading mycotoxins and preparations thereof, can solve problems such as mycotoxins contamination, and achieve the effects of ensuring food safety, low production and use costs, and obvious detoxification effects.

Active Publication Date: 2010-06-09
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to develop and develop bacterial strains capable of degrading mycotoxins and their preparations in view of the widespread contamination of mycotoxins in grain production and feed processing and storage.

Method used

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  • Bacterial strain capable of degrading mold toxin and formulation preparation method thereof
  • Bacterial strain capable of degrading mold toxin and formulation preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Acquisition of A16

[0024] Soil samples were collected from the wheat field of Jiangsu Academy of Agricultural Sciences, and deoxynivalenol was used for multiple subculture enrichment cultures in the laboratory (30°C, once a week), and deoxynivalenol could be degraded. Bacterial suspension of enol, spread the bacterial suspension on LB medium (30°C, 7d), pick different colonies on the plate, and verify the degradation effect of deoxynivalenol one by one Finally, a strain capable of degrading deoxynivalenol was obtained, added glycerol, and stored in a -70°C refrigerator. For this strain, it is defined as A16.

[0025] By measuring the physiological and biochemical characteristics of A16, cloning the 16SrDNA sequence of A16 and sequencing the 16SrDNA sequence, and comparing the sequencing results with BLAST in Genbank, the phylogenetic evolutionary status of A16 was determined. Through the results of the physiological and biochemical characteristics of A16 and the res...

Embodiment 2

[0028] Preparation of liquid inoculum

[0029] Medium: Glucose 1.0%, (NH 4 ) 2 SO 4 0.1%, K 2 HPO 4 0.2%, MgSO 4 0.02%, NaCl 0.01%, CaCO 3 0.3%, peptone 0.05%, the rest is distilled water; the pH value of the medium is 7.2-7.5.

[0030] The A16 strain was activated on a petri dish, and the degradation performance was measured, and it was inoculated on the inclined surface of the test tube to obtain a test tube seed for future use.

[0031] The test tube seed was inoculated into a 1000 mL shake flask containing 200 mL LB, and cultured with constant temperature shaking at 30°C to the logarithmic phase to obtain the strain.

[0032] Use a 500-liter seed tank with a medium feeding volume of 400 liters. After the feeding is completed, 121 ° C high-pressure damp heat sterilization, after cooling to below 35 ° C, inoculate the above-mentioned cultured strains with 10% of the medium in the seed tank. Put it into a seed tank, cultivate it to the logarithmic growth phase, and ob...

Embodiment 3

[0035] Preparation of solid bacterial agent

[0036] The A16 liquid bacterial agent produced in Example 2 is mixed with the sun-dried peat into a solid state, thereby making the A16 solid microbial agent. The peat is provided by Heilongjiang Huamei Peat Co., Ltd.

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Abstract

The invention relates to a mycotoxin degrading bacterial strain and a method for preparing the preparation of the same, and is characterized in that the accession number of the bacterial strain is CCTCC M 208087; the bacterium is short-rod shaped, non spore forming, gram-negative, ivory when cultured in colony in a TSA culture medium and aerobic; catalase-positive, oxidase-negative,urease-positiveand indophenol reaction negative, capable of hydrolysising esculin, incapable of dual hydrolysising Arginine, casein and starch, incapable of decomposing tyrosine and liquefying glutin, capable of using glucose, incapable of using L-arabinose, D-mannose and maltose; the accession number of the strain 16S rDNA in Genbank is EU794908. The invention also provides the method for preparing liquid microbial inoculums, solid microbial inoculums and mycotoxin absorbents with the strain.

Description

technical field [0001] The invention relates to a strain capable of degrading toxins and a preparation method thereof, in particular to a strain capable of degrading mycotoxins and an application thereof. Background technique [0002] Mycotoxins are a class of toxic secondary metabolites with a wide range of chemical structures produced by phytopathogenic fungi infecting cereal crops. They have a wide range of toxic effects on humans and animals, and can cause various symptoms such as cancer and liver toxicity in humans and animals. . At present, there are more than 150 kinds of molds known to produce mycotoxins, and about 300 kinds of mycotoxins. Most of the mycotoxins that are important in feed hygiene come from Aspergilus, Fusarium, Penicillium, etc. These molds can produce aflatoxin, aflatoxin, ochratoxin, zearalenone, deoxynivalenol (DON), T-2 toxin, fumonisin, etc. Mycotoxins. It is estimated that 25% of the world's food crops are contaminated with mycotoxins every...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A23K1/16C12R1/01A23K10/18
Inventor 徐剑宏史建荣陆琼娴祭芳
Owner JIANGSU ACAD OF AGRI SCI
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