Unlock instant, AI-driven research and patent intelligence for your innovation.
A strain of Geotrichum candidum cm1 degrading cephalosporin antibiotics and its application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of cephalosporins and antibiotics, applied in the field of microorganisms, can solve problems such as scarcity of degrading strains
Active Publication Date: 2020-07-21
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, there are very few research reports on the biodegradation of cephalosporin antibiotics at present, and the degrading strains screened are even scarcer, mainly bacteria, which can only degrade one of the cephalosporin antibiotics.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0026] The source of bacteria samples: fresh bacteria residues from an antibiotic manufacturer.
[0027] Potato dextrose medium (PDA): 200g potatoes, 20g glucose, 1L distilled water, natural pH, sterilized at 115°C for 30min. Add 10-15g of agar to the solid medium.
[0028] Martin's Medium: KH 2 PO 4 1.0g, MgSO 4 7H 2 O 0.5g, glucose 10.0g, peptone 5.0g, distilled water 1L, pH natural, sterilized at 115°C for 15min. Add 10g-15g of agar to the solid medium.
[0029] Inorganic salt medium: (NH 4 ) 2 NO 4 1.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, CaCl 2 0.1g, MnSO 4 h 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, pH7.0, sterilize at 121℃ for 15-20min. Add 10g-15g of agar to the solid medium.
[0030] Medium No. Ⅱ: beef extract 1.5g, peptone 6.0g, yeast extract powder 6.0g, glucose 1.0g, water 1L, sterilized at 115°C...
Embodiment 2
[0064] Growth and degradation characteristics of strain CM1
[0065] 1. Growth of strain CM1
[0066] 1. Growth of strain CM1 on different media
[0067] It has been found through experiments that the strain CM1 can grow in various media, such as Martin's medium, potato medium, Chase medium, and inorganic salt medium. Fast growing and easy to cultivate. Streak the strain CM1 on the potato solid medium, and culture it at room temperature (20°C-37°C) for 5d-10d, the strain CM1 can cover a 9cm plate, and its growth curve is shown in Figure 4 .
[0068] 2. Growth experiment of strain CM1 on medium with different concentrations of cephalosporin antibiotics
[0069] Strain CM1 can grow well in the medium with cephalosporin antibiotics as the only carbon source. Prepare cephradine, cephalexin, and cefuroximesodium as the sole carbon source and the concentrations of the three cephalosporin antibiotics are 50mg / L, 100mg / L, 500mg / L, 1000mg / L, 5000mg / L, and 10000mg / L respectively....
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to a strain of Galactomyces candidum CM1 capable of degrading cephalosporinantibiotics, belonging to the technical field of microorganisms. The strain was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection on November 10, 2017, with the preservation number CGMCC NO.14633. The strain is easy to cultivate, grows fast, has the function of degrading cephalosporinantibiotics, and can degrade and eliminate the toxicity and inhibitory effect of cephalosporinantibiotics on Gram-positive bacteria. It can be applied to the production of bacterial agents, to deal with the waste of cephalosporin antibiotic production enterprises and the residual cephalosporin antibiotics in the environment, and to reduce the harm of cephalosporin antibiotics to the environment and human beings.
Description
technical field [0001] The invention relates to a strain of Geotrichum candidum (Galactomyces candidum) CM1 capable of degrading cephalosporin antibiotics, belonging to the technical field of microorganisms. Background technique [0002] Cephalosporin antibiotics are an important class of β-lactam antibiotics discovered after penicillin. They have the advantages of strong antibacterial activity, broad antibacterial spectrum and resistance to penicillinase, and are widely used in the prevention and treatment of human diseases. According to reports, after antibiotics enter the human body and animals, they cannot be completely metabolized and absorbed. 75%-90% of antibiotics will enter the environment with excreta in the form of drug prototypes or metabolites, and the toxicity of some antibiotic metabolites is even higher than matrix. On the other hand, during the fermentation process of cephalosporin antibiotics, a large amount of bacteria residue and waste water will be prod...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
