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136 results about "Cephalosporin Antibiotic" patented technology

A synthetic or semi-synthetic beta-lactam antibiotic derived from Acremonium (Cephalosporium) fungi with bactericidal activity. Cephalosporin antibiotics bind to and inactivate penicillin-binding proteins (PBPs) located on the inner membrane of the bacterial cell wall. Inactivation of PBPs interferes with the cross-linkage of peptidoglycan chains necessary for bacterial cell wall strength and rigidity. This interrupts bacterial cell wall synthesis and results in the weakening of the bacterial cell wall, eventually causing cell lysis.

Concentration detection method for mixed solution of cephalosporin antibiotics based on Raman spectrum

The invention discloses a concentration detection method for a mixed solution of cephalosporin antibiotics based on Raman spectra. The concentration detection method comprises the following steps: (1) measuring a Raman spectrum of a mixed solution of cephalosporin antibiotics with known concentration; (2) preprocessing the Raman spectrum of the mixed solution of the cephalosporin antibiotics with the known concentration in the step (1); (3) processing the preprocessed Raman spectrum of the mixed solution of the cephalosporin antibiotics in the step (2) by a partial least square method so as to obtain a corrected prediction model; (4) acquiring a Raman spectrum of a mixed solution of to-be-measured cephalosporin antibiotics, and carrying out preprocessing; (5) inputting the Raman spectrum preprocessed in the step (4) into the prediction model so as to obtain the concentration of the mixed solution of the cephalosporin antibiotics. According to the concentration detection method, the concentration of the mixed solution of the multivariable cephalosporin antibiotics is detected by virtue of the Raman spectra. The method is quick and accurate, overcomes the limitations of the prior art effectively, and has important significances on fast food safety inspection and real-time monitoring.
Owner:TIANJIN UNIV

Method for synthesizing 7-phenylacetylamino-3-chloromethyl cephalosporin alkyl acid p-methoxybenzyl ester

The invention belongs to the field of cephalosporin antibiotic medicaments, and in particular relates to a method for synthesizing 7-phenylacetylamino-3-chloromethyl cephalosporin alkyl acid p-methoxybenzyl ester (GCLE). The technical proposal is that the method comprises the following steps: reacting penicillin sulfoxide ester with ammonium benzene sulfinate and 2-mercaptobenzothiazole in dichloromethane, and steaming out a solvent at normal pressure to generate aza-cyclobutanone thiosulfinate intermediate; adding dichloromethane to the intermediate after cooling, stirring and introducing saturated brine ice for cooling, adding trichloro isocyanic acid for reaction so as to generate an allylic chlorination product of the aza-cyclobutanone thiosulfinate; and reducing the pressure and drying the allylic chlorination product by distillation, adding dimethyl formamide to the product, stirring and introducing saturated brine ice for cooling, adding ammonia for reaction, adding water and dichloromethane to the mixture, mixing and stirring the mixture, standing for layering, transferring a dichloromethane layer at the bottom layer to another reactor, and steaming out the solvent at the normal pressure to obtain a dry product which is the GCLE. The method has the advantages of mild reaction condition, few reaction steps, simple operation, short production cycle, and improved production efficiency.
Owner:SHANDONG FANGXING SCI & TECH DEV

Method for preparing cephalosporium acremonium proteome

InactiveCN102443047AOptimized Molecular BreedingOptimal Metabolic EngineeringPeptide preparation methodsTotal proteinAntibiotic Y
The invention discloses a method for preparing cephalosporium acremonium proteome, which comprises the following steps that: cephalosporium acremonium thalli are ground into powder with liquid nitrogen, and then, total protein samples are extracted by a trichloroacetic acid (TCA)-acetone precipitation method; the obtained total protein samples are mixed with hydrated solution, hydration sampling is carried out, immobilized pH gradient (IPG) solid phase rubber strips are adopted, next, isoelectric focusing is carried out, the rubber strips are balanced after the isoelectric focusing completion and are then transferred onto sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, electrophoresis is carried out, the electrophoresis is stopped when bromophenol blue indicators reach the bottom edge, and the gel is obtained; and the transmission scanning is carried out with a gel scanning instrument after the obtained gel is dyed, and the obtained images are analyzed by using software. The cephalosporin C has the important place in the cephalosporin antibiotics production, so the study on the cephalosporium acremonium proteome can be carried out, the foundation is laid for optimizing the molecular breeding and the metabolic engineering of cephalosporin producing strains, and the important significance is realized.
Owner:SHANGHAI INST OF PHARMA IND

Detection method for antibiotic resistance genes

The invention provides a detection method for antibiotic resistance genes. The method can simultaneously detect the presence or level of multiple antibiotic resistance genes (such as KPC and other genes capable of causing bacterial drug resistance to carbapenem antibiotics, CTX-M and other genes capable of causing drug resistance to beta-lactam antibiotics, AAC and other genes capable of causing drug resistance to cephalosporin antibiotics, and drug resistance genes capable of causing bacterial drug resistance to other types of antibiotics) in nucleic acid molecules in samples. A probe set anda kit including the probe set of one or more kinds are also provided. The probe set and kit can be used for implementing the method. In addition, the kit is also provided; the kit can simultaneouslydetect the presence or level of the multiple antibiotic resistance genes (such as KPC and other genes capable of causing bacterial drug resistance to carbapenem antibiotics, CTX-M and other genes capable of causing drug resistance to beta-lactam antibiotics, AAC and other genes capable of causing drug resistance to cephalosporin antibiotics and the drug resistance genes capable of causing bacterial drug resistance to other types of antibiotics) in the nucleic acid molecules in the samples in a round of reaction.
Owner:XIAMEN UNIV

Original development quality ceftazidime and medicine preparation thereof

The invention discloses original development quality ceftazidime and a medicine preparation thereof. The third-generation cephalosporin antibiotics active ester midbody key technology and industrialization obtains the second prize of National Scientific and Technological Progress Award. The cephalosporin antibiotics active ester belongs to a key factor for influencing the internal quality of the cephalosporin. A preparation method comprises the following steps that (a) mixed solvents are added into ceftazidime side chain acid, dibenzothiazyl disulfide, aniline and 2-picoline; triethyl phosphate is dripped for reaction; (b) a coarse product is refined to obtain ceftazidime side chain acid active ester, and the first mother liquid is recovered; (c) the material is added into a mixed solvent for neutralizing 7-APCA; triethylamine is dripped; the temperature reduction is performed for crystal separation and filtering to obtain ceftazidime tert-butyl ester; the second mother liquid is recovered; (d) the ceftazidime tert-butyl ester is subjected to hydrolysis and purification, and then, the ceftazidime is obtained. The original development quality ceftazidime has the advantages that high-toxicity triphenylphosphine is not used; waste liquid and waste slag can be sufficiently recovered and reutilized; the method is safe; the cost is low; the yield is high; the industrial production is facilitated.
Owner:广东金城金素制药有限公司 +1
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