19 results about "Cefixime" patented technology

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

Neisseria gonorrhoeae is one of the most common bacterial sexually transmitted infections (STI). Diagnosis depends on standard nucleic acid amplification testing (NAAT), which is impracticable in most low-resource settings, where the prevalence of this STI is highest. Consequently, such areas utilize syndromic management, which misses a high proportion of cases and leads to antibiotic overuse, contributing to the troubling rise of resistance to the commonly prescribed ciprofloxacin, cefixime and ceftriaxone antibiotics for the treatment of N. gonorrhoeae infections. A specific, highly sensitive and cost-effective lateral flow assay is disclosed that utilizes CRISPR-Cas orthologs, multiplex SHERLOCK technology and isothermal amplification via recombinase polymerase amplification (RPA) for the rapid detection of antibiotic resistance to ciprofloxacin, cefixime and / or ceftriaxone at the point of care. This approach has the potential to increase treatment efficacy in the field and mitigate the spread of antibiotic resistance.

The application discloses cefixime granules and a preparation method thereof, and the granules contain cefixime, a filling agent, a binder, a wetting agent, a flavoring agent and pharmaceutically acceptable adjuvants, wherein the filling agent is a calcium hydrogen phosphate-hydroxyethyl cellulose compound. The cefixime granules prepared by the method have the advantages of simple preparation method, stable quality under the conditions of moisture and heat, no degradation, no impurities, good suspension performance, good dissolution performance, long-term storage with qualified and stable quality, simple preparation process and suitability for industrial mass production.

The invention discloses a preparation method and application of an aromatic dicarboxylic acid-terbium complex fluorescent sensor array. The fluorescent sensor array comprises three fluorescent probes, the terbium complexes are respectively a 5-methoxy isophthalic acid-terbium complex, a 5-hexadecyloxy isophthalic acid-terbium complex and a 5-(6 '-cholesterol) hexyloxy isophthalic acid-terbium complex, and the preparation method comprises the following steps: (1) dissolving an aromatic dicarboxylic acid ligand in DMSO (dimethylsulfoxide) to obtain an aromatic dicarboxylic acid ligand mother solution; dissolving a terbium source in distilled water to obtain terbium ion mother liquor; and (2) adding the terbium ion mother liquor into an HEPES buffer solution, adding an aromatic dicarboxylic acid ligand mother liquor into the HEPES buffer solution, and carrying out constant-temperature reaction at 30-40 DEG C for 40-95 minutes to obtain the aromatic dicarboxylic acid-terbium complex. The fluorescent sensor array can be used for detecting tetracycline, cefixime, chloramphenicol and ciprofloxacin, and is high in accuracy and sensitive.

The invention belongs to the technical field of acyclic compounds, and particularly relates to a method for preparing cefixime side chain ring-opening acid by an aqueous phase method. The method comprises the following steps: adding a compound I, a catalyst and a citric acid solution into water for deprotection reaction to obtain a water solution containing a compound II and the catalyst; cooling an aqueous solution containing a compound II and a catalyst, adding an N-chlorosuccinimide aqueous solution to perform chlorination, adding alkali to adjust pH, extracting, and layering to obtain an aqueous phase; and adding an acid into the water phase to adjust the pH value, separating out a solid, carrying out suction filtration, and carrying out vacuum drying to obtain the cefixime side chain open-loop acid. According to the method, water is used as a solvent system, the environmental protection problems of acid waste gas generation, VOCs volatilization and the like in the prior art are completely eradicated, side reactions are few, and the product yield is high.

The application belongs to the technical field of pharmacy and relates to a method for preparing cefixime by using an enzyme method. The method comprises the following steps: performing an enzymatic hydrolysis reaction on cefixime alkyl ester in water in the presence of a hydrolytic enzyme at a pH of 7-8.5 to obtain cefixime. According to the method for preparing cefixime by using the enzyme method, the reaction condition becomes milder, the generation of impurities in the reaction process is effectively controlled, and the product quality and yield of cefixime are both improved.

The utility model discloses a cefixime bulk drug mixing device, which belongs to the technical field of mixing equipment and comprises a base, a first screw rod used for auxiliary adjustment is mounted on the inner side of the base, a servo motor used for providing adjustment power for the first screw rod is mounted on the side face of the base, and an auxiliary block is in threaded connection with the periphery of the first screw rod. The top end of the auxiliary block penetrates through a limiting groove formed in the top end of the base to be connected with a containing table, the top end of the containing table is connected with a linkage plate through a hinge, an open type processing barrel is installed at the top end of the linkage plate, and a second lead screw used for auxiliary adjustment is installed on the inner side of the containing table. A forward and reverse motor providing adjusting power for the second lead screw is installed on the vertical side face of the containing table, the periphery of the second lead screw is in threaded connection with a movable block slidably connected with the inner bottom wall of the containing table, and the top end of the movable block is connected with a movable rod through a hinge. According to the cefixime bulk drug mixing device, automatic and rapid discharging can be achieved, and it is guaranteed that the overall processing speed of the device is not affected.

The invention discloses an archaia adheris DLW198 and application of the archaia adheris DLW198. Belongs to the technical field of antibiotic degradation. According to the present invention, the cefoperazone concentration tolerable by the concorchus chinensis DLW198 is 50-250 mg / L, the inoculation amount of the concorchus chinensis is 5-25%, the degradation temperature is 20-40 DEG C, the pH value is 5-9, and the concorchus chinensis can effectively degrade the cefoperazone under the conditions of the concorchus chinensis DLW198, under the conditions that the temperature is 30 DEG C, the pH value is 7, the inoculum size is 20% and the rotating speed is 200 r / min, the degradation rate of the strain DLW198 to 200 mg / L cefoperazone reaches 92.98% within 16 h. In addition, the strain DLW198 also can be used for degrading cefalexin, ceftriaxone sodium, cefixime and amoxicillin. The strain is relatively high in biological safety, does not harm human health or pollute the environment in the application process, and has important application value.

This invention relates to the field of pharmaceutical chemical synthesis technology, specifically disclosing a method for preparing a stable cefixime side-chain acid active ester. The invention involves adding dibenzothiazole disulfide, cefixime side-chain acid, an organic amine, and triethyl phosphite to a reaction solvent for a condensation reaction; then adding a crystallization solvent to the reaction system, cooling to allow crystallization, separating the solid and liquid phases, and washing the resulting solid with a phosphate buffer to obtain the cefixime side-chain acid active ester; the particle size of the dibenzothiazole disulfide is D90 = 20 μm~45 μm; the pH of the phosphate buffer is neutral. This invention couples the condensation reaction and crystallization process into one step, synergistically adding an organic amine and triethyl phosphite, purifying with a specific neutral phosphate buffer, and controlling the particle size of the raw material DM, achieving product quality assurance throughout the entire process from reaction source to purification. Accelerated testing has confirmed that the sample exhibits high stability.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to cefixime granules and a preparation method thereof. The cefixime granules provided by the invention are prepared from cefixime, a dissolution-promoting and stability-maintaining agent and pharmaceutically acceptable auxiliary materials, wherein the dissolution-promoting and stability-maintaining agent is a combination of sodium caprylate and meglumine; meanwhile, the dissolution-promoting stability-maintaining agent and the cefixime are subjected to a specific pretreatment process, and then the cefixime particles are prepared through a wet granulation process. According to the technical scheme, the dissolution performance and the stability of the cefixime granules are synergistically optimized, and the preparation process is simple, convenient and controllable, does not need to depend on a special high-energy-consumption process, has relatively high industrial feasibility and is beneficial to large-scale production and market promotion.

The application belongs to the technical field of acyclic compounds, and particularly relates to a method for preparing cefixime side chain ring-opening acid by a water phase method. Compound I, a catalyst and a citric acid solution are added in water to perform a deprotection reaction, so as to obtain an aqueous solution containing compound II and the catalyst; the aqueous solution containing compound II and the catalyst is cooled, and then N-chlorosuccinimide aqueous solution is added to perform a chlorination reaction; alkali is added to adjust the pH value; extraction, separation and layering are performed to obtain an aqueous phase; acid is added in the aqueous phase to adjust the pH value, so that solid is precipitated; suction filtration and vacuum drying are performed to obtain cefixime side chain ring-opening acid. The application uses water as a solvent system, so that environmental problems such as generation of acidic waste gas and VOCs volatilization in the existing process are eliminated, the number of side reactions is small, and the product yield is high.

This application relates to the field of continuous crystallization technology and provides a method for continuous crystallization of cefixime. The method includes: preheating a cefixime-containing solution to be crystallized, adding an aqueous hydrochloric acid solution for mixing to obtain a primary mixture; adjusting the pH of the primary mixture and stirring for a first crystallization; continuing to add an aqueous hydrochloric acid solution for mixing to obtain a secondary mixture; adjusting the pH of the secondary mixture, cooling, stirring, and performing a second crystallization to obtain a crystalline liquid; centrifuging the crystalline liquid to obtain a mother liquor; cooling the mother liquor, stirring, and performing a third crystallization to obtain the finished product; or cooling the crystalline liquid, stirring, performing a third crystallization, centrifuging, and then obtaining the finished product. The continuous crystallization method for cefixime described in this application can improve production yield and continuously produce cefixime raw materials with a required particle size distribution.

The invention relates to the technical field of chromatographic detection, in particular to a method for determining the concentration of cefixime in K2EDTA human plasma through liquid chromatography-tandem mass spectrometry. Comprising the following steps: preparing a standard curve solution containing cefixime-13C-15N2 by using K2EDTA (Ethylene Diamine Tetraacetic Acid) human plasma as a matrix, preparing a test solution containing cefixime, and precipitating the solutions by using acetonitrile; determining the concentration of the cefixime test solution after precipitation by using an internal standard method; a liquid chromatography tandem mass spectrometer is used for determination, a mobile phase of liquid chromatography is composed of a mobile phase A and a mobile phase B, the mobile phase A is an aqueous solution containing 10 mM ammonium acetate, and the mobile phase B is acetonitrile. The invention provides an efficient and accurate cefixime concentration detection method by optimizing chromatographic conditions and mass spectrum parameters, so that the accuracy and reliability of data are ensured, and the requirements of biological sample analysis in clinical tests are met.

The invention relates to a preparation method of a cefixime intermediate, which belongs to the technical field of drug intermediate synthesis, and comprises the following steps: 1, synthesizing an oxime ether intermediate by using tert-butyl acetoacetate as an initial raw material and using a two-step one-pot method; in the second step, a chlorination-cyclization one-pot reaction is adopted, wherein the oxime ether intermediate reacts with NOCl generated by hydrochloric acid and tert-butyl nitrite, and a chlorination product is generated; carrying out cyclization reaction with thiourea and triethylamine to obtain an acid intermediate; and 3, in order to avoid decomposition of the product, respectively pulping the acid intermediates-dichloromethane, DM-triethylamine and triphenylphosphine-dichloromethane, and mixing and reacting in the microchannel to finally obtain the cefixime intermediate. The whole synthesis route avoids strong acid and other raw materials with great environmental pollution, avoids generation of byproducts, obtains high-yield and high-purity products, and shows better economic advantages.

The invention relates to a method for preparing a stabilized pharmaceutical composition based on a cefixime crystal form III, and relates to the technical field of medicine preparation. The method comprises the following steps: adding a cefixime crude product into a solvent A to obtain a cefixime solution; adding a seed crystal of the cefixime crystal form III into the cefixime solution, and stirring and mixing; stirring, cooling and crystallizing the cefixime solution containing the seed crystal; carrying out solid-liquid separation on the crystallized solution, collecting solids, washing the solids with a solvent B, and carrying out vacuum drying to obtain the cefixime crystal form III, the preparation method comprises the following steps: uniformly stirring and mixing cefixime crystal form III, a filler, a terpolymer disintegrating agent, an adhesive and a lubricant, preparing particles by adopting a wet granulation process, and then tabletting to obtain the stabilized pharmaceutical composition tablet. The cefixime crystal form III prepared by the invention is high in purity, the degradation rate of effective components of the stabilized pharmaceutical composition is low, and the effectiveness and safety of the medicine can be guaranteed.

The present invention belongs to the technical field of wastewater treatment, and in particular to a method for recycling and treating wastewater produced by active thioester of cefixime side chain. The method comprises the following steps: adjusting the pH of wastewater 1, adding a decolorizing agent and a flocculant to stir, filtering, distilling and recovering acetonitrile by decompression of the filtrate, and concentrating the remaining wastewater to the original 20-30% by negative pressure, centrifuging, and collecting sodium chloride solid; the liquid obtained after centrifugation is concentrated by negative pressure again, and the remaining liquid is collected; water is added to the remaining liquid for extraction, the organic phase is collected, wastewater 2 is added to the organic phase for temperature rise and dissolution, activated carbon is added for filtration, hydrogen peroxide is added dropwise to the filtrate for crystallization, and the reaction is kept warm, and after centrifugation, the solid is collected, methanol is added to the solid for washing, and after centrifugation again, the solid is collected, and 2,2'-dithiodibenzothiazole is dried; the present invention recovers 2,2'-dithiodibenzothiazole from the synthetic residual liquid, reduces the discharge of solid waste in the solvent recovery process, realizes energy conservation and emission reduction, and reduces production costs.

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of cefixime side chain acid active ester based on a supergravity reaction, which comprises the following steps: S1, sequentially adding an organic solvent, cefixime side chain acid, a catalyst and dibenzothiazyl disulfide to the outer side of a rotating packed bed of a supergravity reactor through a liquid inlet, starting the supergravity reactor, and reacting for 2-4 hours; adding a first batch of triethyl phosphite into the middle part of a rotating packed bed of the supergravity reactor through a feeding spray pipe, and carrying out a first-stage supergravity reaction; s2, carrying out a second-stage supergravity reaction; s3, carrying out a third-stage supergravity reaction; and S4, after the third-stage supergravity reaction, obtaining a cefixime side chain acid active ester crude product, and separating and drying to obtain the cefixime side chain acid active ester. According to the present invention, the power energy consumption is low, the methanol is not required to refine after the cefixime side chain acid active ester is synthesized, the loss is reduced, and the obtained product has advantages of high purity, high yield and short reaction time.

The present invention relates to a method for preparing a stabilized pharmaceutical composition based on cefixime crystal form III, and relates to the technical field of drug preparation. A crude cefixime product is added to a solvent A to obtain a cefixime solution; seed crystals of cefixime crystal form III are added to the cefixime solution and stirred and mixed; the cefixime solution containing the seed crystals is stirred, cooled, and crystallized; the crystallized solution is subjected to solid-liquid separation, a solid is collected, the solid is washed with a solvent B, and vacuum dried to obtain cefixime crystal form III; the cefixime crystal form III, a filler, a terpolymer disintegrant, a binder, and a lubricant are stirred and mixed uniformly, prepared into granules using a wet granulation process, and then tabletted to obtain stabilized pharmaceutical composition tablets. The cefixime crystal form III prepared by the present invention has high purity, and the degradation rate of the active ingredients of the stabilized pharmaceutical composition is low, thereby ensuring the effectiveness and safety of the drug.

The application discloses a kind of adhering arrow bacteria DLW198 and application.It belongs to the technical field of antibiotic degradation.The adhering arrow bacteria DLW198 of the application can tolerate the concentration of cefoperazone is 50-250mg / L, the inoculation amount of adhering arrow bacteria is 5%-25%, the degradation temperature is 20-40 DEG C, and pH is 5-9, under the above conditions, adhering arrow bacteria can effectively degrade cefoperazone.Under the conditions of temperature 30 DEG C, pH 7, inoculation amount 20%, and rotation speed 200r / min, the degradation rate of strain DLW198 to 200mg / L cefoperazone reaches 92.98% in 16h.In addition, strain DLW198 can also degrade cephalexin, ceftriaxone sodium, cefixime and amoxicillin.The strain has higher biological safety, and will not endanger human health or pollute the environment in the application process, and has important application value.