37 results about "Amoxicillin" patented technology

The invention provides a method for evaluating the minimum inhibitory concentration of compound amoxicillin powder, and belongs to the technical field of compound amoxicillin, and the method comprises the following steps: (1) simulating a gastric acid environment, and respectively adding different compound amoxicillin powder samples to obtain different sample solutions; (2) simulating a small intestine environment for different sample solutions obtained in the step (1); and (3) sampling different sample solutions after the step (2), measuring the minimum inhibitory concentration, and comparing the effect of the compound amoxicillin powder sample to evaluate. The invention provides a method for evaluating the minimum inhibitory concentration of compound amoxicillin powder. The method is simple to operate and can quickly and accurately evaluate the effect of the compound amoxicillin powder.

This invention discloses a penicillin G acylase mutant SEQ ID NO:3, which can efficiently catalyze the reaction of substrate 6-APA with DHPGM to produce amoxicillin. The synthesis / hydrolysis ratio S / H reaches a maximum of 18.1. When the side chain to parent nucleus ratio is 1.05:1, the conversion rate of parent nucleus 6-APA reaches more than 99.0%. This mutant has high thermal stability, which helps to improve the efficiency of industrial production of amoxicillin.

This invention belongs to the field of product separation technology in enzymatic antibiotic synthesis, and particularly relates to a method for separating and recovering amoxicillin products from a one-step synthesis catalyzed by a Kluyveromycin β-subunit G385 mutant penicillin acylase. This application uses an immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from potassium penicillin. A method for separating amoxicillin and phenylacetic acid is developed for the obtained reaction mixture. The technical solution mainly includes: firstly, separating the immobilized penicillin acylase mutant from the reaction solution by filtration; then separating amoxicillin by crystallization; and finally, separating and recovering phenylacetic acid by toluene extraction, back-extraction, etc. This separation method can rapidly and efficiently separate amoxicillin with a high yield, with an average crystallization rate of 93.22%; simultaneously, the separation and recovery of phenylacetic acid is good; and the extractant toluene can be recycled.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The invention belongs to the technical field of microorganisms, and particularly relates to multi-drug-resistant klebsiella pneumoniae and application thereof in construction of a pneumonia animal model. Specifically, the multi-drug-resistant klebsiella pneumoniae BKP919 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36301, and the multi-drug-resistant klebsiella pneumoniae BKP919 has wide drug resistance to various antibiotics such as amoxicillin, piperacillin and the like. The bacterial liquid of the strain is used for carrying out one-time intratracheal instillation on a mouse in a nasal instillation manner, and a stable severe pneumonia model can be established within 48-72 hours. The model preparation method is simple to operate and high in efficiency, and the model mouse has remarkable pneumonia symptoms, obvious pathological change and good related index stability, and can be used for pathogenesis research and targeted drug development of multi-drug-resistant klebsiella pneumonia infected pneumonia, so that the model has good practical application value.

This invention discloses an amoxicillin capsule and its preparation method, relating to the field of pharmaceutical technology. The preparation method includes the following steps: S1: Wet ball milling of amoxicillin trihydrate and hydroxypropyl methylcellulose E5, followed by spray drying to obtain surface-modified amoxicillin trihydrate micropowder; S2: Mixing the surface-modified amoxicillin trihydrate with a filler; S3: Fluidized bed granulation, including spray granulation using aqueous ethanol as a wetting agent followed by drying; S4: Granulation and mixing, including granulating the dried particles and then adding a disintegrant, a flow aid, and a lubricant; S5: Capsule filling, including capsule filling with controlled ambient humidity. This invention achieves the goals of maintaining the crystal stability of amoxicillin trihydrate, inhibiting hydrothermal degradation, and ensuring dissolution behavior consistent with the original drug by combining wet ball milling of hydroxypropyl methylcellulose E5 with spray drying for surface modification, fluidized bed granulation with aqueous ethanol, and controlling the humidity of the filling environment.

The application belongs to the technical field of medicine, and particularly relates to a compound montmorillonite particle for treating Helicobacter pylori infection and a preparation method thereof. The compound montmorillonite particle for treating Helicobacter pylori infection comprises A component and B component, wherein the A component is prepared from the following raw materials according to weight fractions: montmorillonite 50-65 parts; filler a 5-15 parts; binder a 0.1-1.5 parts; disintegrant 2-7 parts; the B component is prepared from the following raw materials according to weight fractions: amoxicillin 5-10 parts; rifabutin 0.2-0.5 parts; filler b 4-15 parts; binder b 1-6 parts; disintegrant 2-7 parts. The compound montmorillonite particle for treating Helicobacter pylori infection provided by the application has no drug resistance problem, and improves the compliance of patients with dysphagia and children to drug administration; and the application further provides a preparation method thereof, which is simple to operate and stable in process.

The utility model relates to the technical field of canning and weighing, and discloses amoxicillin powder preparation canning and weighing equipment which comprises a base, a frame is fixedly installed at the top of the base, a bottom block is fixedly assembled on the outer wall of the base, a filling hopper is installed at the top of the bottom block, and the filling hopper is fixedly assembled on the outer wall of the base. A cross beam and a controller are fixedly assembled on the outer wall of the frame, and a weighing assembly is arranged on the outer wall of the frame. The bottom of the weighing hopper can be in lap joint with the top of the weighing machine, the weighing machine can weigh the weighing hopper and display the weight on the outer wall of the display screen, and when a filling numerical value is reached, a signal can be transmitted through the controller, so that the driving motor starts to work, and the rotating shaft can drive the rotating handle to rotate; and the clamping block can rotate on the outer wall of the rotating shaft, the weighing hopper is in a dumping shape, amoxicillin powder on the inner wall weighed by the weighing hopper is dumped, and the subpackaging accuracy and the automation degree are improved.

The application belongs to the technical field of enzyme catalysis, and particularly relates to a penicillin acylase for synthesizing beta-lactam antibiotics. Compared with the amino acid sequence shown in SEQ ID NO. 1, the mutation mode of the mutant is F146aK; or F146aK&G385bY; or F146aK&G385bR. The application obtains a penicillin acylase mutant with high hydrolysis activity and synthesis activity and coordinated two enzyme activities by mutating the penicillin acylase from Kluyvera citrophila. The mutant can be used for the synthesis and production of beta-lactam antibiotics, and particularly catalyzes the one-step preparation of amoxicillin from penicillin potassium salt, thereby avoiding the separation of the intermediate 6-APA. The application provides a key enzyme for the efficient preparation of beta-lactam antibiotics, and will greatly promote the innovation of the preparation technology of beta-lactam antibiotics.

This invention belongs to the field of biopharmaceutical technology, and particularly relates to a one-pot method for synthesizing amoxicillin. This invention provides a novel enzymatic synthesis route for amoxicillin, specifically including the following steps: (1) Pretreatment of immobilized penicillin G acylase – soaking PGA washed with deionized water in phosphate buffer; (2) dissolving potassium penicillin G and L-p-hydroxyphenylglycine methyl ester in a phosphate buffer and hexane system to obtain an initial reaction solution; (3) mixing the immobilized penicillin G acylase with the initial reaction solution and reacting to obtain an amoxicillin suspension; (4) filtering out the immobilized penicillin G acylase and washing with deionized water, adding hydrochloric acid until the amoxicillin suspension becomes clear, filtration, pH adjustment, cooling crystallization, washing and drying to obtain crude amoxicillin. The entire preparation process is simple, easy to operate, environmentally friendly, has good substrate conversion effect, and a high amoxicillin yield.

A preparation method of amoxicillin micro-powder belongs to the field of preparation of amoxicillin micro-powder, and comprises the following steps: step 1, adding amoxicillin dissolved clear liquid and ammonia water into a first ultrasonic mixer in a hedging manner; 2, when the liquid level in the first ultrasonic mixer reaches the specified height, mixed liquid in the first ultrasonic mixer is fed into a second mixer; 3, the mixed liquid in the first ultrasonic mixer is injected into a second mixer and collides with obstacles in the second mixer; 4, after all mixed liquid in the first ultrasonic mixer in the second mixer is injected, ammonia water is added again for mixing, and crystallization is continued; and step 5, filtering after the crystallization is completed, and drying the solid to obtain the amoxicillin micro powder. By introducing the ultrasonic oscillator, the mixing efficiency of amoxicillin-dissolved clear liquid and ammonia water can be effectively improved, meanwhile, the crystallization liquid in the first mixer is subjected to ultrasonic crushing, and crystals can be further crushed after the crystallization liquid entering the second mixer along with the mixed liquid in the second mixer collides with a barrier at a high speed.

The application discloses a kind of amoxicillin solubility visual detection methods based on quantitative color scale, specifically related to drug detection field, the present application aims at solving the problems of large standard gradient interval, unstable color development and lack of correction in the existing visual comparison method, method includes: with 0.2M potassium dihydrogen phosphate solution as solvent, preparation amoxicillin saturated solution;Linear regression equation is established, by precisely preparing 9 concentration gradient amoxicillin standard solution, after adding copper sulfate solution, form stable yellow-green turbidity gradient, and draw linear regression equation with absorbance value;Sample determination, the sample liquid is compared with standard gradient and turbidimetric analysis is carried out, the concentration is determined, and the solubility is calculated by combining dilution multiple, the present application converts visual estimation into accurate quantification by high-density gradient standard and linear regression model, significantly reduces matching error, improves anti-interference ability and accuracy, and is suitable for rapid detection in drug research and development and quality control.

The invention provides a method for evaluating compound amoxicillin powder, which belongs to the technical field of medicine detection, and comprises the following steps: detecting the content of an effective component of a medicine, and recording the content as the content A of the effective component; enabling the medicine to sequentially pass through the simulated gastric acid environment and the simulated small intestine environment, detecting the active ingredient content of the medicine after passing through the simulated gastric acid environment, recording the active ingredient content as an active ingredient content B, and detecting the active ingredient content of the medicine after passing through the simulated small intestine environment, recording the active ingredient content as an active ingredient content C; and counting the content change of the effective components of the medicine, and evaluating the compound amoxicillin powder. According to the technical scheme provided by the invention, the purposes of simplifying the evaluation method and reducing the cost are achieved.

The present application relates to a kind of amoxicillin gastric retention sustained-release preparation, which contains amoxicillin, sustained-release material, swelling agent and other pharmaceutical adjuvant, preferably also contains stabilizer or ion regulator.The combination of the above-mentioned adjuvant can be retained in the stomach for up to 24 hours, the complete release time is 10-24h, which can significantly prolong the retention time of amoxicillin in the stomach, has the advantages of fast floating, high effective concentration of drug in the stomach, long stable concentration time, and is beneficial to reduce the frequency of drug administration of patients;Further preferably, it is made into a double-layer tablet containing immediate-release layer, which can quickly reach effective concentration and also can be released slowly and continuously, maintaining effective concentration for a long time;When combined with proton pump inhibitors such as vonolabian, etc., combination kit or compound, only 1-2 times per day is needed, which can further improve the therapeutic effect and patient compliance.

A preparation method of low-water-activity amoxicillin belongs to the field of low-water-activity amoxicillin production, and comprises the following steps: step 1, punching amoxicillin dissolved clear liquid and ammonia water into a first crystallizer for mixing; secondly, the first crystallization liquid obtained in the first crystallizer and ammonia water are injected into a second crystallizer in a hedging mode to be mixed; step 3, transferring a second crystalline liquid obtained in the second crystallizer into a third crystallizer for crystal growing; 4, after crystal growing is completed, centrifugal discharging is conducted, and obtained solids are washed with deionized water; and 5, drying the washed solid to obtain the low-water-activity amoxicillin. According to the preparation method disclosed by the invention, the amoxicillin with relatively high purity and relatively low water activity can be obtained, so that the quality of the amoxicillin can be improved.

This invention discloses a gelling material for treating gastric diseases and its preparation method, belonging to the field of pharmaceutical preparation technology. The gelling material is composed of 8-10g of Codonopsis pilosula extract, 6-10g of Bletilla striata extract, 6-8g of Dioscorea opposita extract, 2-4g of Citrus reticulata peel extract, 2-3g of gelatin, 2-4g of xylitol, 0.1-0.2g of citric acid, and 60.8-73.9g of purified water. As a carrier matrix, the gelling material of this invention exhibits good compatibility with commonly used antacids (such as calcium carbonate), PPIs (such as omeprazole), antibiotics (such as amoxicillin and clarithromycin), and traditional Chinese medicine extracts. During preparation, the above-mentioned active pharmaceutical ingredients (APIs) can be uniformly dispersed in the gel matrix, achieving integrated synergistic treatment of "mucosal protection + acid suppression / bactericidal action" without compromising their chemical stability or causing burst release problems, providing an ideal platform for developing compound gastric disease preparations.

A mutant penicillin acylase and uses thereof are provided. Compared to the amino acid sequence set forth in SEQ ID NO: 1, the mutant includes at least one mutation selected from the group consisting of: F146αK, F24βR, F71βY, N241βK, G385βY, and G385βR. By introducing mutations into the penicillin acylase derived from Kluyvera citrophila, the invention obtains a mutant enzyme exhibiting enhanced and well-coordinated hydrolytic and synthetic activities. It can be used for the synthesis of β-lactam antibiotics, particularly for the one-step preparation of amoxicillin from penicillin potassium salt, thereby avoiding the isolation of the intermediate 6-APA. The present invention provides a key enzyme for the efficient production of β-lactam antibiotics and is poised to significantly advance the innovation of their manufacturing technology.

The utility model provides a veterinary amoxicillin powder mixing device, which belongs to the technical field of veterinary drug powder mixing, and comprises a stirring shell, and the upper inner wall of the stirring shell is rotatably connected with a plurality of stirring rods through a rotating shaft; the motor is fixedly connected to the upper end of the stirring shell, and the output end of the motor is fixedly connected with the rotating shaft; when amoxicillin and other materials need to be mixed, the materials are poured into the stirring shell, the rotating shaft and the multiple stirring rods are driven by the motor to rotate, the multiple materials in the stirring shell are stirred and mixed, then the materials are discharged through the pouring-out groove, and in the process, the materials are filtered and screened through the filter screen, so that the materials are mixed uniformly. And larger particles and impurities can stay at the upper end of the filter screen to finish screening and filtering.

The application discloses a high water-soluble amoxicillin soluble powder and a preparation method thereof. The application belongs to the technical field of veterinary drug preparations. The application adopts supercritical CO2 eutectic of amoxicillin trihydrate and L-malic acid, and is matched with gas explosion modified starch, hydrophobic nano-SiO2 and polyaspartic acid sodium. The supercritical eutectic and the gas explosion modified starch are combined to make the powder have super-high solubility; the PASP competitively combines metal ions, and the nano-SiO2 synergistically adsorbs metal ions to improve the hard water stability of the powder; the eutectic hydrogen bond protection and the nitrogen filling packaging make the L-malic acid hydrogen bond stabilize the beta-lactam ring, the nitrogen environment inhibits oxidation to improve long-term stability and reduce the degradation rate; the high-pressure homogenization microspheres and the spray drying porous structure improve the water penetration and the rapid dissolution performance of the powder, and provide technical support for the preparation of the high water-soluble stable amoxicillin powder.

This invention discloses amoxicillin-loaded self-assembled nanoparticles, their preparation method, and applications, belonging to the field of biomedical material preparation technology. The preparation method includes the following steps: mixing ginkgolic acid and anhydrous N,N-dimethylformamide, adding a condensing agent, and stirring to obtain a reactant; adding D-citrulline, adjusting the pH to alkaline by adding an organic base, reacting at room temperature, adding ultrapure water to the reaction solution, adjusting the pH to acidic, extracting, collecting the organic phase, washing, drying, filtering, concentrating under reduced pressure, dialysis, and lyophilizing to obtain a ginkgolic acid-D-citrulline conjugate; mixing with a self-assembly carrier material and amoxicillin, forming a nano-dispersion through self-assembly in a solvent system, and lyophilizing to obtain amoxicillin-loaded self-assembled nanoparticles. These amoxicillin-loaded self-assembled nanoparticles have small particle size, good dispersibility, and high stability, which can improve the delivery effect and application performance of amoxicillin in Staphylococcus aureus biofilm-associated infections.

The invention discloses a construction method and application of a multi-organ development injury animal model based on non-coding long-chain RNA (Ribonucleic Acid). According to the present invention, it is found that the expression level of LncRNA URS0000A7E830 is significantly increased compared to the normal control group in the embryonic period amoxicillin exposed zebra fish model, and the URS0000A7E830 siRNA microinjection intervention is performed on the fertilized one-cell period zebra fish embryo based on the embryonic period amoxicillin exposed zebra fish model so as to effectively improve the multi-organ differentiation damage. The model established by the invention is novel, reliable, simple and convenient, and has important significance for illuminating the occurrence mechanism of the development injury of multiple organs and determining clinical early warning and intervention targets.

The application provides a kit for detecting penicillin and amoxicillin resistance of streptococcus suis and application thereof, and belongs to the technical field of molecular biology detection. The kit comprises a primer pair and AlwNI restriction endonuclease; the primer pair comprises primers PBP1a-2083F and PBP1a-2083R, and the sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The kit is used for detecting penicillin and amoxicillin resistance of streptococcus suis, has the advantages of high specificity, high sensitivity, low detection cost and low technical requirement, and provides reliable technology for monitoring penicillin and amoxicillin resistance of streptococcus suis in clinic.

The utility model relates to the field of natural compound chemistry and pharmacology, namely to 2-(4,5-Dichloroisothiazole-3-carbonyl)-7-methoxy-1methyl-1,2,3,4-tetrahydroisoquinolin-6-yl 4,5-dichloroisothiazole-3-carboxylate (1). The purpose of the utility model is to expand the arsenal of domestic natural medicines based on alkaloids and their derivatives with antimicrobial activity. Based on the available alkaloid salsolin, its derivative, 2-(4,5-Dichloroisothiazole-3-carbonyl)-7-methoxy-1-methyl1,2,3,4-tetrahydroisoquinolin-6-yl 4,5-dichloroisothiazole-3-carboxylate, which has pronounced antimicrobial activity, was synthesized. It has been experimentally established that 2-(4,5-Dichloroisothiazole-3-carbonyl)-7-methoxy-1-methyl1,2,3,4-tetrahydroisoquinolin-6-yl 4,5-dichloroisothiazole-3-carboxylate has pronounced antimicrobial activity against the gram-positive test strain Staphylococcus aureus and exceeds the comparison drug amoxicillin by 1.6 times.

The invention relates to the field of production equipment, and discloses compound amoxicillin powder bag sorting equipment which comprises a mounting frame, a conveying belt is mounted in the mounting frame, a deviation rectifying assembly is arranged in the mounting frame and used for rectifying deviation of packaged products, and a flattening assembly is arranged outside the mounting frame and used for flattening the packaged products. The deviation rectifying assembly is arranged on the front side of the middle of the mounting frame, the flattening assembly is used for flattening the packaging products, the driving assembly is arranged outside the mounting frame and used for driving the deviation rectifying assembly and the flattening assembly, and the sorting assembly is arranged on the front side of the middle of the mounting frame and used for sorting the unqualified packaging products detected by the flattening assembly. The technical scheme of linkage of the flattening assembly and the deviation rectifying assembly is adopted, the effect of automatically adjusting the flatness of the packaging products is achieved, more accurate and stable packaging product conveying can be achieved through a linkage mechanism, and the production efficiency and the automation degree are improved.

The present application relates to the technical field of pharmaceutical preparation, and in particular to a preparation method of amoxicillin dispersible tablets, comprising the following steps: S1, mixing based on an internal complex cross-linking disintegrant system to form an internal water absorption channel structure, and obtaining first mixed material; S2, granulating after adjusting granulation parameters based on the bulk density and first angle of repose of the first mixed material, and obtaining first mixed granules; S3, mixing based on an external complex cross-linking disintegrant system to form a granule inter-disintegration network structure, and obtaining second mixed material; and S4, tabletting after adjusting the tabletting pressure based on the second angle of repose of the second mixed material and the particle size distribution of the first mixed granules. The present application significantly improves the disintegration performance, dissolution rate and quality stability of the amoxicillin dispersible tablets by constructing intelligent process control logic.

The invention discloses application of Fe-Cu bimetallic nano-enzyme to detection of beta-lactam antibiotics in food, and belongs to the technical field of nanotechnology and colorimetric sensing. The Fe-Cu bimetallic nano material prepared by the invention has three enzyme activities of peroxidase-like, laccase-like and oxidases-like, and a three-enzyme-activity channel colorimetric sensing array is constructed based on the inhibition effect of different beta-lactam antibiotics on the oxidation of a chromogenic substrate; the simple, convenient, rapid and reliable detection on the four beta-lactam antibiotics such as penicillin G, amoxicillin, oxacillin and carbenicillin is realized. The method specifically comprises the following steps: A, preparing Fe-Cu bimetallic nano-enzyme; b, optimizing reaction conditions of the sensing array; c, on the basis of the prepared Fe-Cu bimetallic nano-enzyme, constructing a beta-lactam antibiotic colorimetric sensing detection array; d, carrying out discriminant analysis on the colorimetric fingerprint spectrum obtained by detection by adopting a classical pattern recognition method; and E, detecting an actual sample.

The invention relates to a heterotrophic nitrification-aerobic denitrification strain capable of synchronously degrading antibiotics and application of the heterotrophic nitrification-aerobic denitrification strain, belonging to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 8, 2025, and the preservation number is CGMCC No.33884. The invention also discloses a method for preparing the strain. Klebsiella oxytoca TY is a bacterium capable of realizing synchronous denitrification and antibiotic degradation by utilizing multiple substrates, is high in growth and propagation speed, and can realize effective removal of inorganic nitrogen and organic nitrogen in wastewater by utilizing an organic carbon source and phosphate under the conditions that the C / N (mass ratio) is 35, the initial ammonia nitrogen concentration is 80mg / L, the P / N (mass ratio) is 0.1, the pH is 7.5 and the temperature is 30 DEG C; meanwhile, the strain achieves the purpose of removing 30 mg / L of amoxicillin through biological adsorption and biological degradation. A theoretical basis is provided for the strain in the fields of denitrification of antibiotic-containing wastewater, antibiotic degradation, water body remediation and the like.

This invention belongs to the field of enzyme catalysis technology, and particularly relates to an artificially designed penicillin G acylase, its encoding nucleotide, and its applications. Compared with the amino acid sequence shown in SEQ ID NO.1, the mutant exhibits the following mutations: G385βY; or G385βR; or F146αK&N241βK&G385βY. This invention, through mutation of penicillin acylase derived from Kluyvera citrophila, yielded a penicillin acylase mutant with strong hydrolytic and synthetic activities, exhibiting coordinated activities of both enzymes. It can be used in the synthetic production of β-lactam antibiotics, particularly catalyzing the one-step preparation of amoxicillin from potassium penicillin, avoiding the separation of the intermediate 6-APA. This invention provides a key enzyme for the efficient preparation of β-lactam antibiotics, which will greatly advance the technological innovation of β-lactam antibiotic preparation.