72 results about "Amoxicillin" patented technology

The invention provides a method for evaluating the minimum inhibitory concentration of compound amoxicillin powder, and belongs to the technical field of compound amoxicillin, and the method comprises the following steps: (1) simulating a gastric acid environment, and respectively adding different compound amoxicillin powder samples to obtain different sample solutions; (2) simulating a small intestine environment for different sample solutions obtained in the step (1); and (3) sampling different sample solutions after the step (2), measuring the minimum inhibitory concentration, and comparing the effect of the compound amoxicillin powder sample to evaluate. The invention provides a method for evaluating the minimum inhibitory concentration of compound amoxicillin powder. The method is simple to operate and can quickly and accurately evaluate the effect of the compound amoxicillin powder.

This invention discloses a penicillin G acylase mutant SEQ ID NO:3, which can efficiently catalyze the reaction of substrate 6-APA with DHPGM to produce amoxicillin. The synthesis / hydrolysis ratio S / H reaches a maximum of 18.1. When the side chain to parent nucleus ratio is 1.05:1, the conversion rate of parent nucleus 6-APA reaches more than 99.0%. This mutant has high thermal stability, which helps to improve the efficiency of industrial production of amoxicillin.

The invention belongs to the technical field of enzyme catalysis, and particularly relates to recombinant penicillin G acylase, encoding nucleotide and application of the recombinant penicillin G acylase. Compared with an amino acid sequence shown in SEQ ID NO. 1, the mutant has the advantages that the mutation mode is F24 beta R; or F146 [alpha] K amp; f24 [beta] R; or F146 [alpha] K amp; f24 [beta] R amp; f71 [beta] Y; or F146 [alpha] K amp; f24 [beta] R amp; f71 [beta] Y amp; n241 [beta] K amp; and G385 [beta] Y. According to the invention, wild type penicillin acylase is mutated to obtain penicillin acylase with coordinated enzymatic activity of hydrolysis and synthesis. The method can be used for synthesis production of beta-lactam antibiotics, especially for one-step preparation of amoxicillin by catalyzing penicillin potassium salt and the like, and separation of an intermediate 6-APA is avoided. The invention provides a key enzyme for efficient preparation of beta-lactam antibiotics, and greatly promotes the innovation of a beta-lactam antibiotic preparation technology.

This invention provides a MnO2@CDs composite material for the visual detection of amoxicillin and its preparation method, relating to the field of analytical detection technology. The MnO2@CDs constructed in this invention can be used for both fluorescence and colorimetric detection of amoxicillin. Due to the resonant energy transfer between CDs and MnO2, the fluorescence of CDs is quenched. In the presence of AMO, MnO2 is degraded by AMO, releasing CDs, and its blue fluorescence is restored. In colorimetric detection, MnO2@CDs exhibit excellent oxidase-like properties, capable of oxidizing colorless TMB to yellow TMB. 2+ AMO has strong reducing properties and inhibits oxidation reactions. In the presence of AMO, it can promote the oxidation of TMB. 2+ When AMO is reduced to TMB, the solution color gradually changes from yellow to colorless as the AMO concentration increases, resulting in a vibrant color change and enabling the visual detection of AMO.

This invention belongs to the field of product separation technology in enzymatic antibiotic synthesis, and particularly relates to a method for separating and recovering amoxicillin products from a one-step synthesis catalyzed by a Kluyveromycin β-subunit G385 mutant penicillin acylase. This application uses an immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from potassium penicillin. A method for separating amoxicillin and phenylacetic acid is developed for the obtained reaction mixture. The technical solution mainly includes: firstly, separating the immobilized penicillin acylase mutant from the reaction solution by filtration; then separating amoxicillin by crystallization; and finally, separating and recovering phenylacetic acid by toluene extraction, back-extraction, etc. This separation method can rapidly and efficiently separate amoxicillin with a high yield, with an average crystallization rate of 93.22%; simultaneously, the separation and recovery of phenylacetic acid is good; and the extractant toluene can be recycled.

The application provides a preparation method of a magnetic multi-level hole iron-nitrogen-carbon catalyst and belongs to the field of new advanced oxidation catalyst materials. The preparation method is as follows: (1) dissolving iron nitrate nonahydrate in N,N-dimethylformamide, dissolving 2-amino terephthalic acid in N,N-dimethylformamide, and then mixing the two liquids; (2) transferring the mixed liquid into a closed reaction container and heating into a gel in a blast drying oven; (3) washing with N,N-dimethylformamide and ethanol, drying in a fume hood at room temperature to obtain a multi-level hole metal organic framework gel FeBDC-NH2; (4) transferring into a porcelain boat and placing into a tube furnace to react at high temperature under an anaerobic atmosphere, so that the magnetic multi-level hole iron-nitrogen-carbon catalyst is obtained. The catalyst prepared by the method has rich defects and excellent electron transfer capacity, the multi-level hole structure of the material accelerates the mass transfer efficiency of the catalytic reaction, the material magnetism facilitates recycling, and the material can directionally catalyze the degradation of amoxicillin by a non-free radical path through persulfate.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The application provides a penicillin G acylase Ax The PGA mutant and the expression plasmid, the genetically engineered bacteria and the application belong to the technical field of biological catalysts. The PGA mutant is obtained by mutation of any one or more of the following positions of the amino acid sequence shown in SEQ ID NO: 1: (1) methionine at the 103th position is replaced by cysteine, (2) tyrosine at the 424th position is replaced by phenylalanine, (3) tyrosine at the 442th position is replaced by phenylalanine, and (4) tyrosine at the 451th position is replaced by alanine. The obtained penicillin G acylase Ax The PGA mutant has high enzyme activity and can be applied to high-conversion-rate and high-yield synthesis of beta-lactam antibiotics such as benzylpenicillin and amoxicillin.

The invention belongs to the technical field of enzyme catalysis, and particularly relates to penicillin acylase for synthesizing beta-lactam antibiotics. Compared with an amino acid sequence shown in SEQ ID NO. 1, the mutant has the advantages that the mutation mode is F146 alphaK; or F146 [alpha] K amp; g385 [beta] Y; or F146 [alpha] K amp; g385 [beta] R is shown in the description. According to the penicillin acylase mutant disclosed by the invention, penicillin acylase from kluyvera citrophila (Kluyvera citrophila) is mutated, so that the penicillin acylase mutant which is strong in hydrolytic activity and synthetic activity and is coordinated in two enzyme activities is obtained. The method can be used for synthesis production of beta-lactam antibiotics, especially for one-step preparation of amoxicillin by catalyzing penicillin potassium salt and the like, and separation of an intermediate 6-APA is avoided. The invention provides a key enzyme for efficient preparation of beta-lactam antibiotics, and greatly promotes the innovation of a beta-lactam antibiotic preparation technology.

The invention belongs to the technical field of microorganisms, and particularly relates to multi-drug-resistant klebsiella pneumoniae and application thereof in construction of a pneumonia animal model. Specifically, the multi-drug-resistant klebsiella pneumoniae BKP919 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36301, and the multi-drug-resistant klebsiella pneumoniae BKP919 has wide drug resistance to various antibiotics such as amoxicillin, piperacillin and the like. The bacterial liquid of the strain is used for carrying out one-time intratracheal instillation on a mouse in a nasal instillation manner, and a stable severe pneumonia model can be established within 48-72 hours. The model preparation method is simple to operate and high in efficiency, and the model mouse has remarkable pneumonia symptoms, obvious pathological change and good related index stability, and can be used for pathogenesis research and targeted drug development of multi-drug-resistant klebsiella pneumonia infected pneumonia, so that the model has good practical application value.

This invention discloses an amoxicillin capsule and its preparation method, relating to the field of pharmaceutical technology. The preparation method includes the following steps: S1: Wet ball milling of amoxicillin trihydrate and hydroxypropyl methylcellulose E5, followed by spray drying to obtain surface-modified amoxicillin trihydrate micropowder; S2: Mixing the surface-modified amoxicillin trihydrate with a filler; S3: Fluidized bed granulation, including spray granulation using aqueous ethanol as a wetting agent followed by drying; S4: Granulation and mixing, including granulating the dried particles and then adding a disintegrant, a flow aid, and a lubricant; S5: Capsule filling, including capsule filling with controlled ambient humidity. This invention achieves the goals of maintaining the crystal stability of amoxicillin trihydrate, inhibiting hydrothermal degradation, and ensuring dissolution behavior consistent with the original drug by combining wet ball milling of hydroxypropyl methylcellulose E5 with spray drying for surface modification, fluidized bed granulation with aqueous ethanol, and controlling the humidity of the filling environment.

The invention belongs to the technical field of enzyme catalysis, and particularly relates to a high-activity penicillin G acylase mutant, encoding nucleotide and application of the high-activity penicillin G acylase mutant. Compared with an amino acid sequence shown in SEQ ID NO. 1, the mutant has the advantages that the mutation mode is F71betaY; or F146 [alpha] K amp; f71 [beta] Y; or F146 [alpha] K amp; f71 [beta] Y amp; n241 [beta] K; or F146 [alpha] K amp; f71 [beta] Y amp; n241 [beta] K amp; and G385 [beta] Y. Through site-directed mutagenesis, the penicillin acylase mutant with strong hydrolytic activity and synthetic activity and coordinated two enzyme activities is obtained. The method can be used for synthesis production of beta-lactam antibiotics, especially for one-step preparation of amoxicillin by catalyzing penicillin potassium salt and the like, and separation of an intermediate 6-APA is avoided. The invention provides a key enzyme for efficient preparation of beta-lactam antibiotics, and greatly promotes the innovation of a beta-lactam antibiotic preparation technology.

The application belongs to the technical field of medicine, and particularly relates to a compound montmorillonite particle for treating Helicobacter pylori infection and a preparation method thereof. The compound montmorillonite particle for treating Helicobacter pylori infection comprises A component and B component, wherein the A component is prepared from the following raw materials according to weight fractions: montmorillonite 50-65 parts; filler a 5-15 parts; binder a 0.1-1.5 parts; disintegrant 2-7 parts; the B component is prepared from the following raw materials according to weight fractions: amoxicillin 5-10 parts; rifabutin 0.2-0.5 parts; filler b 4-15 parts; binder b 1-6 parts; disintegrant 2-7 parts. The compound montmorillonite particle for treating Helicobacter pylori infection provided by the application has no drug resistance problem, and improves the compliance of patients with dysphagia and children to drug administration; and the application further provides a preparation method thereof, which is simple to operate and stable in process.

The utility model relates to the technical field of canning and weighing, and discloses amoxicillin powder preparation canning and weighing equipment which comprises a base, a frame is fixedly installed at the top of the base, a bottom block is fixedly assembled on the outer wall of the base, a filling hopper is installed at the top of the bottom block, and the filling hopper is fixedly assembled on the outer wall of the base. A cross beam and a controller are fixedly assembled on the outer wall of the frame, and a weighing assembly is arranged on the outer wall of the frame. The bottom of the weighing hopper can be in lap joint with the top of the weighing machine, the weighing machine can weigh the weighing hopper and display the weight on the outer wall of the display screen, and when a filling numerical value is reached, a signal can be transmitted through the controller, so that the driving motor starts to work, and the rotating shaft can drive the rotating handle to rotate; and the clamping block can rotate on the outer wall of the rotating shaft, the weighing hopper is in a dumping shape, amoxicillin powder on the inner wall weighed by the weighing hopper is dumped, and the subpackaging accuracy and the automation degree are improved.

The invention discloses a colorimetric / Tyndall effect dual-signal mode detection system based on nanogold, which comprises a light source part, a cuvette and a spectrograph, under the action of the light source part, transmission light and scattered light of a reaction system in the cuvette are collected through the spectrograph, and an absorption spectrum and a scattered spectrum are obtained; a reaction system is contained in a cuvette, the reaction system comprises nucleic acid aptamer functionalized nanogold, a buffer solution and an analyte, and the nucleic acid aptamer functionalized nanogold is obtained by modifying nanogold with oligonucleotide PolyA10-T5-Apt. A nucleic acid aptamer corresponding to amoxicillin is embedded into a three-section type oligonucleotide sequence, namely a polyadenine-polythymine-nucleic acid aptamer (polyA-T-Apt), oligonucleotide is modified on the surface of nanogold through a polyA fragment, the amoxicillin is detected at the same time by utilizing the colorimetric characteristic and the Tyndall effect of the nanogold, and dual-signal mode output is achieved.

The application belongs to the technical field of enzyme catalysis, and particularly relates to a penicillin acylase for synthesizing beta-lactam antibiotics. Compared with the amino acid sequence shown in SEQ ID NO. 1, the mutation mode of the mutant is F146aK; or F146aK&G385bY; or F146aK&G385bR. The application obtains a penicillin acylase mutant with high hydrolysis activity and synthesis activity and coordinated two enzyme activities by mutating the penicillin acylase from Kluyvera citrophila. The mutant can be used for the synthesis and production of beta-lactam antibiotics, and particularly catalyzes the one-step preparation of amoxicillin from penicillin potassium salt, thereby avoiding the separation of the intermediate 6-APA. The application provides a key enzyme for the efficient preparation of beta-lactam antibiotics, and will greatly promote the innovation of the preparation technology of beta-lactam antibiotics.

The present invention relates to a compound pharmaceutical composition and its application in preventing Helicobacter pylori infection, and more specifically, to the compound pharmaceutical composition, pharmaceutical preparation, preparation method and use. The composition comprises at least two selected from the following components: (I) vonoprazan or a pharmaceutically acceptable salt thereof; (II) amoxicillin or a pharmaceutically acceptable salt thereof; (III) clarithromycin; and (IV) a bismuth agent. The composition prepared by compounding different anti-infective active ingredients can not only effectively solve the problems of patient compliance and medication convenience, such as reducing the dosage of tablets and avoiding patients from taking the wrong tablets, missing a dose, or taking more than one dose, but also unexpectedly improve the stability and safety of the preparation, and is particularly conducive to reducing the content of harmful impurities.

This invention belongs to the field of biopharmaceutical technology, and particularly relates to a one-pot method for synthesizing amoxicillin. This invention provides a novel enzymatic synthesis route for amoxicillin, specifically including the following steps: (1) Pretreatment of immobilized penicillin G acylase – soaking PGA washed with deionized water in phosphate buffer; (2) dissolving potassium penicillin G and L-p-hydroxyphenylglycine methyl ester in a phosphate buffer and hexane system to obtain an initial reaction solution; (3) mixing the immobilized penicillin G acylase with the initial reaction solution and reacting to obtain an amoxicillin suspension; (4) filtering out the immobilized penicillin G acylase and washing with deionized water, adding hydrochloric acid until the amoxicillin suspension becomes clear, filtration, pH adjustment, cooling crystallization, washing and drying to obtain crude amoxicillin. The entire preparation process is simple, easy to operate, environmentally friendly, has good substrate conversion effect, and a high amoxicillin yield.

A preparation method of amoxicillin micro-powder belongs to the field of preparation of amoxicillin micro-powder, and comprises the following steps: step 1, adding amoxicillin dissolved clear liquid and ammonia water into a first ultrasonic mixer in a hedging manner; 2, when the liquid level in the first ultrasonic mixer reaches the specified height, mixed liquid in the first ultrasonic mixer is fed into a second mixer; 3, the mixed liquid in the first ultrasonic mixer is injected into a second mixer and collides with obstacles in the second mixer; 4, after all mixed liquid in the first ultrasonic mixer in the second mixer is injected, ammonia water is added again for mixing, and crystallization is continued; and step 5, filtering after the crystallization is completed, and drying the solid to obtain the amoxicillin micro powder. By introducing the ultrasonic oscillator, the mixing efficiency of amoxicillin-dissolved clear liquid and ammonia water can be effectively improved, meanwhile, the crystallization liquid in the first mixer is subjected to ultrasonic crushing, and crystals can be further crushed after the crystallization liquid entering the second mixer along with the mixed liquid in the second mixer collides with a barrier at a high speed.

The application discloses a kind of amoxicillin solubility visual detection methods based on quantitative color scale, specifically related to drug detection field, the present application aims at solving the problems of large standard gradient interval, unstable color development and lack of correction in the existing visual comparison method, method includes: with 0.2M potassium dihydrogen phosphate solution as solvent, preparation amoxicillin saturated solution;Linear regression equation is established, by precisely preparing 9 concentration gradient amoxicillin standard solution, after adding copper sulfate solution, form stable yellow-green turbidity gradient, and draw linear regression equation with absorbance value;Sample determination, the sample liquid is compared with standard gradient and turbidimetric analysis is carried out, the concentration is determined, and the solubility is calculated by combining dilution multiple, the present application converts visual estimation into accurate quantification by high-density gradient standard and linear regression model, significantly reduces matching error, improves anti-interference ability and accuracy, and is suitable for rapid detection in drug research and development and quality control.

The application discloses a marine source beta-lactam antibiotic biodegradation bacterium and application thereof. The marine source beta-lactam antibiotic biodegradation bacterium is isolated and identified from coastal seawater and has the ability of degrading beta-lactam antibiotics. Research shows that the DT01 strain has certain degradation effect on beta-lactam antibiotics, such as penicillin potassium, amoxicillin and ceftriaxone sodium, wherein the DT01 has excellent degradation capacity on penicillin potassium; and the DT01 strain has good temperature and pH adaptability and penicillin potassium resistance, can grow rapidly in a harsh environment and can be better used for the treatment of antibiotic environmental pollution, thereby providing a new effective repair scheme for beta-lactam antibiotic pollution and making contribution to the biological treatment of antibiotic pollution.

The utility model relates to the technical field of medicine processing, and discloses an anti-caking amoxicillin powder mixing device which comprises four supporting legs, the top ends of the four supporting legs are fixedly connected with a processing tank, a motor drives a rotating rod to rotate clockwise to drive a first stirring blade to stir amoxicillin powder in a processing cavity, and the processing tank is fixedly connected with a second stirring blade. A rotating rod rotates clockwise to drive a driving gear to rotate, so that the driving gear drives a first driven gear to rotate, the first driven gear rotates to drive a first inner gear to enable an upper rotating ring to rotate anticlockwise, and the upper rotating ring rotates anticlockwise to drive two transmission rods and a lower rotating ring to rotate; and in the rotating process of two rotating rods, through the meshing effect of a first driven gear and a second inner gear, the transmission rods can rotate clockwise besides rotating anticlockwise around an upper rotating ring, so that a plurality of second stirring blades rotate along with the transmission rods, and amoxicillin powder in a processing cavity is stirred.

The invention provides a method for evaluating compound amoxicillin powder, which belongs to the technical field of medicine detection, and comprises the following steps: detecting the content of an effective component of a medicine, and recording the content as the content A of the effective component; enabling the medicine to sequentially pass through the simulated gastric acid environment and the simulated small intestine environment, detecting the active ingredient content of the medicine after passing through the simulated gastric acid environment, recording the active ingredient content as an active ingredient content B, and detecting the active ingredient content of the medicine after passing through the simulated small intestine environment, recording the active ingredient content as an active ingredient content C; and counting the content change of the effective components of the medicine, and evaluating the compound amoxicillin powder. According to the technical scheme provided by the invention, the purposes of simplifying the evaluation method and reducing the cost are achieved.

The present application relates to a kind of amoxicillin gastric retention sustained-release preparation, which contains amoxicillin, sustained-release material, swelling agent and other pharmaceutical adjuvant, preferably also contains stabilizer or ion regulator.The combination of the above-mentioned adjuvant can be retained in the stomach for up to 24 hours, the complete release time is 10-24h, which can significantly prolong the retention time of amoxicillin in the stomach, has the advantages of fast floating, high effective concentration of drug in the stomach, long stable concentration time, and is beneficial to reduce the frequency of drug administration of patients;Further preferably, it is made into a double-layer tablet containing immediate-release layer, which can quickly reach effective concentration and also can be released slowly and continuously, maintaining effective concentration for a long time;When combined with proton pump inhibitors such as vonolabian, etc., combination kit or compound, only 1-2 times per day is needed, which can further improve the therapeutic effect and patient compliance.

The invention relates to a method for treating medical waste through cooperation of spectrums and steam, and belongs to the technical field of medical waste treatment. According to the treatment method, the medical waste is treated through the cooperation of ultraviolet rays, near infrared rays and steam, the microorganism inactivation rate of the pet medical waste can be remarkably increased, the degradation rate of amoxicillin can be increased, biological membranes and chemical pollutants on the surfaces of the sharp instruments can be effectively removed, and the biological loading capacity of the treated sharp instruments is smaller than or equal to 10 CFU / g; meanwhile, compared with a traditional high-temperature treatment method, the treatment method has the advantages that the treatment period is shortened, energy consumption is reduced, and the method is suitable for on-site treatment of medical waste.

A preparation method of low-water-activity amoxicillin belongs to the field of low-water-activity amoxicillin production, and comprises the following steps: step 1, punching amoxicillin dissolved clear liquid and ammonia water into a first crystallizer for mixing; secondly, the first crystallization liquid obtained in the first crystallizer and ammonia water are injected into a second crystallizer in a hedging mode to be mixed; step 3, transferring a second crystalline liquid obtained in the second crystallizer into a third crystallizer for crystal growing; 4, after crystal growing is completed, centrifugal discharging is conducted, and obtained solids are washed with deionized water; and 5, drying the washed solid to obtain the low-water-activity amoxicillin. According to the preparation method disclosed by the invention, the amoxicillin with relatively high purity and relatively low water activity can be obtained, so that the quality of the amoxicillin can be improved.

The present invention discloses a strain of Stenotrophomonas maltophilia and its application in the treatment of polluted environments. The present invention is the first to isolate and identify a strain of Stenotrophomonas maltophilia capable of degrading antibiotics from seawater samples. Studies have shown that the DA02 strain can degrade the antibiotics penicillin potassium, amoxicillin and ceftriaxone sodium, among which it has a highly efficient degradation effect on ceftriaxone sodium antibiotics, and also has a good degradation effect in high-concentration antibiotic drugs. Its degradation rate increases with the increase of antibiotic concentration, and the degradation effect remains stable over time. It shows that the DA02 strain, as a highly efficient ceftriaxone sodium degrading bacterium, is not affected by the concentration of the drug, and can be better applied to the degradation of high-concentration antibiotics in antibiotic-contaminated environments, providing more efficient treatment methods and approaches for antibiotic-contaminated environments.

This invention discloses a gelling material for treating gastric diseases and its preparation method, belonging to the field of pharmaceutical preparation technology. The gelling material is composed of 8-10g of Codonopsis pilosula extract, 6-10g of Bletilla striata extract, 6-8g of Dioscorea opposita extract, 2-4g of Citrus reticulata peel extract, 2-3g of gelatin, 2-4g of xylitol, 0.1-0.2g of citric acid, and 60.8-73.9g of purified water. As a carrier matrix, the gelling material of this invention exhibits good compatibility with commonly used antacids (such as calcium carbonate), PPIs (such as omeprazole), antibiotics (such as amoxicillin and clarithromycin), and traditional Chinese medicine extracts. During preparation, the above-mentioned active pharmaceutical ingredients (APIs) can be uniformly dispersed in the gel matrix, achieving integrated synergistic treatment of "mucosal protection + acid suppression / bactericidal action" without compromising their chemical stability or causing burst release problems, providing an ideal platform for developing compound gastric disease preparations.

The invention belongs to the technical field of sterilization and bacteriostasis products, and particularly discloses a composition of vitamin B7 (biotin) and compound amoxicillin and application of the composition. Vitamin B7 basically has no inhibition effect on Escherichia coli BW25113, but the effect of vitamin B7 combined with compound amoxicillin is more remarkable than that of single compound amoxicillin, and the use amount of compound amoxicillin can be reduced and the bactericidal effect can be guaranteed through combination of vitamin B7 and compound amoxicillin. Therefore, the composition provided by the invention can reduce the use amount of compound amoxicillin, and reduce the use of antibiotics and the generation of drug resistance. The effects are also applicable to drug-resistant escherichia coli, the application of the compound amoxicillin in sterilization is further expanded, and the compound amoxicillin has good popularization value.

A mutant penicillin acylase and uses thereof are provided. Compared to the amino acid sequence set forth in SEQ ID NO: 1, the mutant includes at least one mutation selected from the group consisting of: F146αK, F24βR, F71βY, N241βK, G385βY, and G385βR. By introducing mutations into the penicillin acylase derived from Kluyvera citrophila, the invention obtains a mutant enzyme exhibiting enhanced and well-coordinated hydrolytic and synthetic activities. It can be used for the synthesis of β-lactam antibiotics, particularly for the one-step preparation of amoxicillin from penicillin potassium salt, thereby avoiding the isolation of the intermediate 6-APA. The present invention provides a key enzyme for the efficient production of β-lactam antibiotics and is poised to significantly advance the innovation of their manufacturing technology.