The invention discloses an
aldehyde group mesoporous
molecular sieve used for immobilization of a biological
enzyme and a preparation method thereof. In the preparation method, a 60 Co-
gamma ray pre-
irradiation grafting acrolein technique or gamma-
aldehyde propyl trimethoxy
silane is used for performing functionalized modification on the surface of the mesoporous
molecular sieve, an
aldehyde propyl functional group with a shorter
chain length is introduced to the surface of the mesoporous
molecular sieve, the influences, caused by the surface functionalizing process, on the aperture, the specific area and the pore volume of the mesoporous molecular
sieve are reduced as much as possible, and further activation is not needed to ensure that the biological
enzyme is directly immobilized on the surface of the mesoporous molecular
sieve in a covalent bonding mode, and the performance of the
immobilized enzyme is improved. The aldehyde group mesoporous molecular
sieve can be applied to the immobilization of water-soluble enzymes such as
penicillin acylase, glucose
isomerase, transglucosidase, trypsase,
amylase and the like, and is particularly suitable for the immobilization of the
penicillin acylase; and the obtained
immobilized enzyme has the activity of 8895 U / g, and after 10 times of recycling, the
immobilized enzyme maintains 92 percent of the initial activity.