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Aramagnetic aldehyde group mesoporous molecular sieve for immobilized biological enzymes, and preparation method thereof

A mesoporous molecular sieve, aldehyde-based mesoporous technology, applied in biochemical equipment and methods, immobilized enzymes, molecular sieve compounds, etc., to reduce clogging, large specific surface area, and improve separation efficiency.

Inactive Publication Date: 2012-10-03
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through a selective etching process, large-scale nanometer γ-Fe was fabricated using mesoporous silicon 2 o 3 Particle coating to obtain hollow magnetic mesoporous silicon microsphere composite material, which can be used as an adsorbent to quickly separate two antibiotics, tetracycline and sulfamethazine, in the water system. The pore size of the synthesized material is about 3nm, which is also not suitable for macromolecular enzymes Adsorption

Method used

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  • Aramagnetic aldehyde group mesoporous molecular sieve for immobilized biological enzymes, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 0.365g FeCl 3 ·6H 2 O and 0.208g FeSO 4 ·7H 2 O(Fe 3+ / Fe 2+ The molar ratio is 1.8:1) dissolved in 10mL of water and heated to 80°C, adjusted to a pH of about 11 with concentrated ammonia water, aged at this temperature for 2 hours, cooled to room temperature and washed with water until neutral to obtain 0.15g of Fe 3 o 4 Nanoparticles are then dispersed in 150mL of ethanol solution, 0.290g of γ-aminopropyltriethoxysilane is added, and reacted at room temperature under vigorous stirring conditions for 10 hours, and finally the ethanol solution is removed under an external magnetic field to obtain aminopropyl triethoxysilane surface-modified Fe 3 o 4 Nanoparticles.

[0041] 0.85g of the aldehyde-based mesoporous molecular sieve prepared in the comparative example and 0.15g of aminopropyl surface-modified Fe 3 o 4 The nanoparticles were respectively dispersed in 25mL of water, and then mixed uniformly and reacted at room temperature for 10 hours. After the reac...

Embodiment 2

[0043] Change the weight of the aldehyde-based mesoporous molecular sieve into 0.45g in Example 1, and other preparation processes are the same as in Example 1, and the prepared paramagnetic aldehyde-based mesoporous molecular sieve is used for the immobilization of penicillin acylase, and the obtained The activity of the immobilized enzyme was 9621U / g, and after 10 cycles of use, the immobilized enzyme retained 87.6% of its initial activity.

Embodiment 3

[0045] Change the weight of the aldehyde-based mesoporous molecular sieve into 0.55g and the Fe of aminopropyl group surface modification in embodiment 1 3 o 4 The weight of the nanoparticles was changed to 0.30g, and other preparation processes were the same as in Example 1. The prepared paramagnetic aldehyde-based mesoporous molecular sieve was used for the immobilization of penicillin acylase, and the activity of the immobilized enzyme obtained was 8733U / g , after 10 cycles of use, the immobilized enzyme retained 86.6% of its initial activity.

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Abstract

The invention belongs to the technical field of biology, and discloses an aramagnetic aldehyde group mesoporous molecular sieve for immobilizing biological enzymes, and a preparation method thereof. According to the invention, gamma aldehyde glycidoxy propyl trimethoxy silane is utilized to introduce aldehyde groups onto the surface of the mesoporous molecular sieve, and then a covalent bond is utilized to immobilize aramagnetic Fe3O4 nano particles and biological enzymes onto the outer surface and the inner surface of the mesoporous molecular sieve respectively, wherein the aramagnetic Fe3O4 nano particles are subjected to gamma-amino propyl triethoxy silane surface modification and have the particle sizes larger than the pore diameter of the mesoporous molecular sieve, as a result, specially-structured aramagnetic immobilized enzymes can be prepared and are separated from the liquid phase system easily under the action of an external magnetic field, and further, the property and the separation efficiency of the immobilized enzymes are improved. The aramagnetic aldehyde group mesoporous molecular sieve can be used for immobilization of water-soluble biological enzymes such as penicillin acylase, glucose isomerase, glucosylase, trypsin and amylase, the activity of the prepared aramagnetic immobilized penicillin acylase is 9901 U / g, and 91.2% of the initial activity is retained after ten times of cycle use.

Description

technical field [0001] The invention relates to a paramagnetic aldehyde-based mesoporous molecular sieve used for immobilizing biological enzymes and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Penicillin acylase (EC 3.5.1.11, enzyme molecular size: ) is the most critical enzyme in the production of semi-synthetic β-lactam antibiotics. It can catalyze the hydrolysis of penicillin and its cyclic acid to remove the side chain, and produce the important intermediate 6-aminopenicillium of semi-synthetic β-lactam antibiotics Alkanoic acid (6-APA) and 7-amino-3-desacetoxycephalosporanic acid (7-ADCA) can also catalyze the condensation of 6-APA and 7-ADCA with side chains to produce a variety of semi-synthetic β-endo Amide antibiotics (such as Ampicillin, Amoxicillin, Cephalexin and Cefadroxil, etc.). [0003] With a large population in my country, there is a great demand for semi-synthetic β-lactam antibiotics. The produc...

Claims

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Application Information

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IPC IPC(8): C12N11/00C01B37/00C01B39/00
Inventor 郭杨龙杨玲陈林一弘高振源卢冠忠詹望成王筠松郭耘张志刚
Owner EAST CHINA UNIV OF SCI & TECH
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