12results about How to "Improve synthesis ability" patented technology

The invention discloses a method for regulating and controlling efficient conversion of methanol based on (p) ppGpp signal molecules. Specifically, by regulating synthesis and hydrolysis balance of (p) ppGpp signal molecules in microbial cells and combining promoter engineering and nutrition condition optimization, methanol metabolic pathway key gene expression and amino acid metabolic flux are enhanced, and the methanol utilization efficiency and target product synthesis capacity of microorganisms are remarkably improved. The regulation and control mechanism of the (p) ppGpp on methanol conversion under the stress of a low nitrogen source is defined for the first time, efficient conversion of methanol to organic acids such as acetic acid and butyric acid is realized, a brand new strategy is provided for preparation of high-added-value chemicals through methanol bioconversion, and the method has the advantages of being economical, environmentally friendly and high in conversion efficiency and has a wide application prospect.

The present application relates to the technical fields of synthetic biology and microbial metabolic engineering, in particular to a substrate-specific division of labor Saccharomyces cerevisiae mixed bacterial system, a construction method thereof and application of beta-carotene production, wherein a substrate utilization non-crossing Saccharomyces cerevisiae mixed bacterial system is established by constructing a glucose-specific utilization strain YG1 and a lactic acid-acetic acid-specific utilization strain YA2; wherein the YG1 realizes glucose-specific utilization by knocking out the ADY2 gene, the YA2 realizes efficient utilization of lactic acid and acetic acid by integrating the SO1522 gene and the ACKA-PTA acetic acid assimilation pathway, and the HXT7 and GAL2 sites are modified; the mixed bacterial system constructed by the present application can realize the synergistic utilization of mixed carbon sources such as glucose, lactic acid and acetic acid, and improve the beta-carotene synthesis efficiency and fermentation stability.

The utility model provides a quantitative feeding device for organic polymer synthesis, which comprises a reaction kettle, a blanking hopper and a blanking pipe, and the blanking pipe is fixedly communicated with the bottom of the blanking hopper. The utility model relates to the technical field of organic polymer synthesis. Through the arrangement of the transmission mechanism, after material particles are placed in the discharging hopper, the motor is started to drive the first transmission wheel to rotate, the first transmission wheel drives the second transmission wheel to rotate through the belt, and when the second transmission wheel rotates, the second transmission wheel is driven to rotate through the belt; when the spherical check block rotates to the position where the discharging hole faces upwards, materials in the discharging hopper can pass through the discharging hole and then enter the discharging hopper through the discharging pipe, when the spherical check block rotates to other positions, discharging is not carried out, and intermittent quantitative discharging is achieved through sequential circulation. And material accumulation is avoided, so that the synthesis effect is improved.

The application provides a method for biosynthesis of linoleic acid-rich oil by Yarrowia lipolytica. The recombinant Yarrowia lipolytica is constructed by two levels of gene operation, the first level aims to improve the proportion of linoleic acid in oil, and the second level aims to improve the biosynthesis capacity of total oil. The construction method of the Yarrowia lipolytica provided by the application is simple, and the constructed Yarrowia lipolytica can biosynthesize linoleic acid-rich oil efficiently, on the one hand, the oil synthesis capacity is improved, and on the other hand, the proportion of linoleic acid in oil is also significantly improved, so that the Yarrowia lipolytica can replace the plant-derived linoleic acid oil and be applied in food and industry.

This invention discloses a method for modifying a high-yield L-arginine-producing strain and its application in production, belonging to the field of bioengineering technology. This invention integrates E. coli-derived argininosuccinate synthase at the genomic level of E. coli, constructing an argininosuccinate synthase mutant. argG S130N , argG Y132F , argG K133R , argG Y132F / K133R This invention significantly enhanced the conversion of aspartic acid and citrulline to L-arginine, constructing a highly efficient L-arginine-producing strain that is plasmid-free, contains no resistance genes, and requires no induction. The recombinant strain constructed in this invention accumulated 108.5 g / L of L-arginine in a 5L fermenter after 48 hours of fermentation.

The invention discloses a targeted compound probiotic preparation for producing SOD (superoxide dismutase) eggs, which is prepared from the following active ingredients in percentage by mass: 5 to 15 percent of bacillus licheniformis, 3 to 10 percent of pseudomonas strain NJ-62, 15 to 30 percent of saccharomyces boulardii, 10 to 20 percent of lactobacillus plantarum, 8 to 15 percent of bacillus subtilis, 10 to 20 percent of candida utilis and 5 to 15 percent of lactobacillus acidophilus. The invention relates to the technical field of healthy breeding of livestock and poultry and production of functional food, in particular to integrated production of'safety 'and'function', specifically, targeted biological detoxification and nutrition metabolism induction technologies are deeply integrated for the first time, and complex probiotic groups remove antibiotics, heavy metals and other endogenous and exogenous pollutants in advance, so that a'clean 'physiological environment is created for laying hens; on the basis, efficient synthesis of SOD is specifically induced, and the problem of potential safety hazards of the functional eggs is fundamentally solved.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

The application discloses a live broadcast background fusion method and system, a storage medium and a live broadcast device, which are used for realizing higher quality of synthesis effect and enhancing the reality and immersion of live broadcast content. The method comprises the following steps: acquiring a recording and broadcasting material, and performing image segmentation on the recording and broadcasting material to obtain a foreground object and a background image in the recording and broadcasting material; performing image understanding on the foreground object, and converting the foreground object into a first 3D point cloud according to the result of image understanding; acquiring a real-time portrait, and acquiring a second 3D point cloud of the real-time portrait; and synthesizing a target live broadcast image according to the first 3D point cloud, the second 3D point cloud and the background image.

This invention relates to a composition for repairing the lipid barrier and its application in sensitive skin care, belonging to the field of cosmetic technology. The composition includes *Plasmodium purpureus* extract, flaxseed extract, senna-based glycosides, and oat extract. The rational combination of these four components not only promotes lipid synthesis but also ensures effective lipid transport and prevents the oxidative degradation of newly synthesized lipids. By covering the entire lipid metabolism process, it achieves a long-lasting and stable barrier repair effect, and can be applied to the repair of the lipid barrier in people with sensitive skin. Efficacy testing shows that the *Plasmodium purpureus* extract, flaxseed extract, senna-based glycosides, and oat extract in the composition can synergistically enhance the composition's antioxidant and lipid synthesis-promoting capabilities, while effectively promoting skin barrier repair and increasing skin moisture content, exhibiting significant skin-soothing and repairing effects, making it highly suitable for people with sensitive skin.

The application discloses a strain for significantly improving HSAF yield under iron limitation and application thereof, and the genetically engineered bacterium is obtained by knocking out or silencing an iron response transcription factor Fur gene of L.enzymogenes OH11, and the nucleotide sequence of the Fur gene is shown as SEQ ID NO.1.Compared with a wild type strain, the mutant strain has no significant difference in growth in an iron deficiency or low-iron culture medium, and the HSAF synthesis capacity is greatly improved.The HSAF yield of the mutant strain in the iron deficiency culture medium reaches 162.34+5.84 mg / L, which is 3.60 times of that of the wild type strain.Therefore, the technical scheme of the application provides a more direct and effective way for improving the HSAF yield of L.enzymogenes OH11 in the iron deficiency or low-iron environment.

The invention discloses a core-shell structure carbon-coated nickel-neodymium alloy catalyst as well as a preparation method and application thereof, and relates to the technical field of catalysts. The preparation method comprises the following steps: dispersing a soluble nickel salt, a soluble neodymium salt, glucose and titanium dioxide in a mixed solvent of water and ethylene glycol, carrying out a heating reaction, and roasting the product of the heating reaction to obtain the core-shell structure carbon-coated nickel-neodymium alloy catalyst. According to the core-shell structure carbon-coated nickel-neodymium alloy catalyst disclosed by the invention, nano titanium dioxide is taken as a substrate, and directional regulation and control of adsorption and desorption behaviors of reactants on the surface of the catalyst are realized by finely regulating and controlling electronic microenvironments of nickel and neodymium active centers. The reaction path is effectively guided to proceed in an expected direction, so that the synthesis efficiency of the high-carbon alcohol is remarkably enhanced. Experimental results show that the catalyst has excellent catalytic performance in an ethanol water phase coupling reaction, the yield of C4 and higher alcohols can reach 61.1%, and the selectivity can reach 98.8%.

The present application relates to the technical field of metabolic engineering bacteria, and provides an engineered bacterium for efficiently utilizing methanol to produce pyrroloquinoline quinone and a construction method thereof. MutS Then, the bacterium is inoculated into a culture medium containing methanol, and is directionally cultured by increasing the methanol concentration in a 2 g / L gradient MutS The engineered bacterium obtained by gene back complementation. The engineered bacterium strain has not only significantly improved the tolerance and utilization efficiency of methanol, but also realized the significant improvement of PQQ yield by enhancing the synthesis capacity of methanol dehydrogenase coenzyme PQQ. The obtained engineered bacterium strain effectively solves the problem of slow growth of the original strain under the condition of high-concentration methanol, and has important industrial application value and prospect.