52results about How to "Improve synthesis ability" patented technology

The invention discloses a recombinant neurospora crassicola strain for producing astaxanthin as well as a construction method and application of the recombinant neurospora crassicola strain. The recombinant neurospora crassa contains key enzymes beta-carotene ketolase and beta-carotene hydroxylase in a heterologous astaxanthin synthetic pathway, and through pathway expression of a targeted subcellular compartment, crosstalk among metabolites is effectively prevented, metabolic flux of a biosynthetic pathway is coordinated, and the activity of the neurospora crassa is improved. The effective contact between the heterologous protein and the substrate in the astaxanthin synthetic route is promoted, the sporulation capability of the heterologous protein is improved, the astaxanthin production and storage capability of the neurospora crassiforme is effectively improved, and the method has relatively high popularization and application values.

The invention discloses recombinant escherichia coli for producing N-acetylneuraminic acid as well as a construction method and application of the recombinant escherichia coli, and belongs to the technical field of genetic engineering. According to the invention, 6-phosphofructokinase is knocked out, glycerol kinase is introduced into a glycerol kinase mutant, a diffusion transporter is promoted by glucose to replace PTS phosphotransferase I, and a promoter is optimized, so that recombinant escherichia coli can grow by double carbon sources of glucose and glycerol, N-acetylneuraminic acid is synthesized, and meanwhile, the generation amount of acetic acid is extremely low. On the basis, a 6-phosphofructokinase coding gene pfkB is knocked out, a heterologous ED pathway from pseudomonas mobilis is introduced, and the yield under the pore plate level is further increased to 9.44 g / L through copy number optimization and RBS strength optimization.

The invention discloses a method for promoting diabetic fracture healing by using bone marrow mesenchymal stem cell exosomes, which comprises the following steps: separating MSCs (mesenchymal stem cells) from the bone marrow of a diabetic patient, culturing and extracting the exosomes; the method comprises the following steps: extracting an exosome from a bone fracture part, performing quantitative analysis on the extracted exosome, injecting the exosome into the bone fracture part in a manner of intravenous injection, local injection or minimally invasive injection, continuously treating, promoting proliferation and differentiation of osteoblasts by miRNA or mRNA in the exosome through targeting cells, enhancing angiogenesis, and further accelerating fracture healing. According to the exosome, proliferation and differentiation of osteoblasts are promoted through miRNA or mRNA in the exosome, the synthesis capacity of bone matrixes of the osteoblasts is enhanced, so that fracture healing is accelerated, meanwhile, the exosome provides necessary blood supply for fracture healing by adjusting vascular endothelial cells and promoting angiogenesis, and the repair capacity of fracture parts is effectively improved.

The invention provides a key enzyme for synthesizing butanediamine and application of the key enzyme, and belongs to the technical field of bioengineering. The invention provides a gene for synthesizing ornithine decarboxylase. The nucleotide sequence of the ornithine decarboxylase is shown as SEQ ID NO. 7. By further optimizing the reaction system, the butanediamine synthesis capability is improved, and butanediamine can be synthesized more efficiently.

The invention provides a diagnosis and treatment image data desensitization method based on cross-modal adversarial example generation, and relates to the technical field of image data processing, and the method comprises the steps: obtaining a target category label of a given attacker classifier, and generating a fine-grained text description based on the target category label; converting the text description into specific visual representation by using a cross-modal method; forming an adversarial example image by adding perturbations to the visual representation using different adversarial example generation methods; and covering the diagnosis and treatment image data with the adversarial example image to enable the diagnosis and treatment image data to avoid classification of the classifier in the absence of clean samples. According to the invention, the problem that the existing diagnosis and treatment image data desensitization method has strong dependence on source data and cannot meet fine-grained semantic requirements is solved.

The invention discloses construction and application of a recombinant escherichia coli engineering strain with high yield of D-pantothenic acid, and belongs to the technical field of genetic engineering and biological engineering. According to the invention, acetic acid branch pathway genes are knocked out, so that accumulation of precursor pyruvic acid is improved; carrying out free expression and integrated expression on panBC respectively; by weakening a cofactor 5, 10-CH2-THF degradation pathway and strengthening an de novo synthesis pathway of the cofactor 5, 10-CH2-THF, supply of 5, 10-CH2-THF is promoted, and the yield of the constructed engineering strain D-pantothenic acid can reach 4.83 g / L. The Escherichia coli recombinant strain with high yield of D-pantothenic acid, constructed by the invention, lays a foundation for subsequent biosynthesis of D-pantothenic acid and derivatives, and has potential value and significance for development of synthetic biology.

The invention provides a shooting method, electronic equipment and a computer readable medium, and the shooting method comprises the steps: reading and playing a basic accompaniment audio under the condition that a shooting starting instruction is received, and shooting a first video; separating the first segment of video to obtain a corresponding video picture and a corresponding audio; under the condition that an instruction of continuing to shoot is received, playing the audio of the ith segment of video, and shooting the (i + 1) th segment of video; separating from the (i + 1) th segment of video to obtain a corresponding video picture and a corresponding audio; wherein i is any integer larger than or equal to 1 and smaller than N, and N is the segment number of the video needing to be shot; under the condition that an instruction of finishing shooting is received, displaying video pictures from the first video segment to the Nth video segment on a display screen, and receiving layout adjustment parameters; and according to the layout adjustment parameter, storing the video picture in the first video segment to the video picture in the Nth video segment and the audio of the Nth video segment as a target video.

The embodiment of the invention provides application of an extract in preparation of a medicine or a non-medicine composition for improving mitochondrial activity. The mitochondria with improved activity can maintain its function and activity when facing pressure to ensure normal operation of cells without adverse effects of external or internal pressure.

The invention discloses a method for regulating and controlling efficient conversion of methanol based on (p) ppGpp signal molecules. Specifically, by regulating synthesis and hydrolysis balance of (p) ppGpp signal molecules in microbial cells and combining promoter engineering and nutrition condition optimization, methanol metabolic pathway key gene expression and amino acid metabolic flux are enhanced, and the methanol utilization efficiency and target product synthesis capacity of microorganisms are remarkably improved. The regulation and control mechanism of the (p) ppGpp on methanol conversion under the stress of a low nitrogen source is defined for the first time, efficient conversion of methanol to organic acids such as acetic acid and butyric acid is realized, a brand new strategy is provided for preparation of high-added-value chemicals through methanol bioconversion, and the method has the advantages of being economical, environmentally friendly and high in conversion efficiency and has a wide application prospect.

The present application relates to the technical fields of synthetic biology and microbial metabolic engineering, in particular to a substrate-specific division of labor Saccharomyces cerevisiae mixed bacterial system, a construction method thereof and application of beta-carotene production, wherein a substrate utilization non-crossing Saccharomyces cerevisiae mixed bacterial system is established by constructing a glucose-specific utilization strain YG1 and a lactic acid-acetic acid-specific utilization strain YA2; wherein the YG1 realizes glucose-specific utilization by knocking out the ADY2 gene, the YA2 realizes efficient utilization of lactic acid and acetic acid by integrating the SO1522 gene and the ACKA-PTA acetic acid assimilation pathway, and the HXT7 and GAL2 sites are modified; the mixed bacterial system constructed by the present application can realize the synergistic utilization of mixed carbon sources such as glucose, lactic acid and acetic acid, and improve the beta-carotene synthesis efficiency and fermentation stability.

The invention relates to an acremonium terricola fusion strain and a fermentation culture and application thereof, and belongs to the technical field of strain fermentation. The contents of active components such as cordycepin, polysaccharide and the like in secondary metabolites of the acremonium terricola F2652 are obviously increased, so that the conversion and enrichment of the polysaccharide of the acremonium terricola strain can be realized while the content of a symbolic component cordycepin of cordyceps fungi is increased. The acremonium terricola F2652 is subjected to liquid submerged fermentation to obtain a fermentation culture, the advantages of amphiphilic parents are fused, the excellent compound polysaccharide synthesis capacity is reserved, the good cordycepin accumulation potential is also fused, and the acremonium terricola culture with the high cordycepin content can be developed; the practical application of the feed additive as a functional feed additive in different animals can be enriched.

The utility model provides a quantitative feeding device for organic polymer synthesis, which comprises a reaction kettle, a blanking hopper and a blanking pipe, and the blanking pipe is fixedly communicated with the bottom of the blanking hopper. The utility model relates to the technical field of organic polymer synthesis. Through the arrangement of the transmission mechanism, after material particles are placed in the discharging hopper, the motor is started to drive the first transmission wheel to rotate, the first transmission wheel drives the second transmission wheel to rotate through the belt, and when the second transmission wheel rotates, the second transmission wheel is driven to rotate through the belt; when the spherical check block rotates to the position where the discharging hole faces upwards, materials in the discharging hopper can pass through the discharging hole and then enter the discharging hopper through the discharging pipe, when the spherical check block rotates to other positions, discharging is not carried out, and intermittent quantitative discharging is achieved through sequential circulation. And material accumulation is avoided, so that the synthesis effect is improved.

The application provides a method for biosynthesis of linoleic acid-rich oil by Yarrowia lipolytica. The recombinant Yarrowia lipolytica is constructed by two levels of gene operation, the first level aims to improve the proportion of linoleic acid in oil, and the second level aims to improve the biosynthesis capacity of total oil. The construction method of the Yarrowia lipolytica provided by the application is simple, and the constructed Yarrowia lipolytica can biosynthesize linoleic acid-rich oil efficiently, on the one hand, the oil synthesis capacity is improved, and on the other hand, the proportion of linoleic acid in oil is also significantly improved, so that the Yarrowia lipolytica can replace the plant-derived linoleic acid oil and be applied in food and industry.

The present application discloses a processing method and device for video synthesis based on personalized voice, which obtains data to be processed and a video object in an authorized state, and performs intelligent audit on the data to be processed through intelligent audit technology to obtain an audit result. If the audit result is legal data to be processed, the sound line of the video object is simulated through preset simulation rules to obtain the target voice, and the target voice is synthesized with the video corresponding to the video object to obtain a personalized video. Through the above scheme, there is no need to manually intercept and splice personalized voice word by word. It is only necessary to use a deep learning method to simulate the voice line of the video object to synthesize the personalized voice, and synthesize it with the video corresponding to the subsequent video object, thereby improving the effect, diversity and fun of personalized voice and video synthesis. In addition, the compliance of the content input by the user is reviewed through intelligent audit technology, and there is no need for manual participation in the review, thereby improving the efficiency of the review.

The utility model discloses a 1536-hole DNA (deoxyribonucleic acid) automatic liquid separation synthesis plate. The synthesis plate comprises a body (1), the 1536 synthesis chambers (2) are arranged on the body (1) in a matrix manner, and a slope is formed at the opening part of each synthesis chamber (2); the synthesis column (3) is arranged in the synthesis chamber (2) and comprises an inlet part (31) connected with the bottom of the synthesis chamber (2) and an assembly part (32) used for assembling a CPG carrier, and the diameter of the inlet part (31) is gradually reduced from the joint to the tail end of the synthesis chamber (2); and an outlet part (33). According to the 1536-hole DNA automatic liquid separation synthesis plate provided by the utility model, the synthesis capacity per unit area is greatly improved, and the slope is arranged at the opening part of the synthesis cavity to increase the diameter of the opening part, so that liquid can slide into the synthesis cavity after falling on the body and then smoothly enters the synthesis column, and the liquid separation on the synthesis plate body can be realized; a liquid spraying or immersion type liquid adding mode is compatible, so that the synthesis efficiency is greatly improved.

This invention discloses a method for modifying a high-yield L-arginine-producing strain and its application in production, belonging to the field of bioengineering technology. This invention integrates E. coli-derived argininosuccinate synthase at the genomic level of E. coli, constructing an argininosuccinate synthase mutant. argG S130N , argG Y132F , argG K133R , argG Y132F / K133R This invention significantly enhanced the conversion of aspartic acid and citrulline to L-arginine, constructing a highly efficient L-arginine-producing strain that is plasmid-free, contains no resistance genes, and requires no induction. The recombinant strain constructed in this invention accumulated 108.5 g / L of L-arginine in a 5L fermenter after 48 hours of fermentation.

The invention discloses a targeted compound probiotic preparation for producing SOD (superoxide dismutase) eggs, which is prepared from the following active ingredients in percentage by mass: 5 to 15 percent of bacillus licheniformis, 3 to 10 percent of pseudomonas strain NJ-62, 15 to 30 percent of saccharomyces boulardii, 10 to 20 percent of lactobacillus plantarum, 8 to 15 percent of bacillus subtilis, 10 to 20 percent of candida utilis and 5 to 15 percent of lactobacillus acidophilus. The invention relates to the technical field of healthy breeding of livestock and poultry and production of functional food, in particular to integrated production of'safety 'and'function', specifically, targeted biological detoxification and nutrition metabolism induction technologies are deeply integrated for the first time, and complex probiotic groups remove antibiotics, heavy metals and other endogenous and exogenous pollutants in advance, so that a'clean 'physiological environment is created for laying hens; on the basis, efficient synthesis of SOD is specifically induced, and the problem of potential safety hazards of the functional eggs is fundamentally solved.

The invention discloses a solid electrolyte sulfide synthesis device with an automatic proportioning function, and belongs to the technical field of solid electrolyte processing. Various pre-prepared raw materials are put into a plurality of feeding cylinders, and a double-shaft motor is controlled to work to drive a top cover to rotate through a fixed cover; the top cover drives a plurality of feeding barrels to rotate around the sulfide synthesis tank, gears outside the feeding barrels are meshed with gear rings, so that the gears can drive the feeding barrels to rotate, and feeding spiral blades can uniformly convey raw materials downwards until the raw materials fall into the sulfide synthesis tank; the feeding cylinder can rotate around the sulfide synthesis tank and also can rotate, so that the raw materials can be uniformly distributed on the periphery in the sulfide synthesis tank, the traditional mode of pouring various raw materials into the sulfide synthesis tank together is abandoned, the various raw materials can be in full contact, the mixing effect is improved, the reaction time is greatly shortened, and the production efficiency is improved. The efficient and stable synthesis work of the solid electrolyte sulfide is facilitated.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

The utility model discloses a gravity liquid supply turning blowing device for a chip synthesizer, which comprises a cabin body, and a chip placing seat, a turning cover mechanism, a rotating shaft, a turning driving unit, a gas and liquid supply supporting mechanism and a locking mechanism which are arranged in the cabin body, two ends of the rotating shaft are rotatably connected with two ends of the cabin body, and the turning cover mechanism is driven by the turning driving unit to rotate; the chip placing seat is fixed relative to the cabin body through a horizontal positioning mechanism, and the horizontal positioning mechanism is mounted on the outer wall of the cabin body; the cover turning mechanism comprises a cover frame, an upper cover and a plurality of pressing units, and acting force can be applied to the upper cover in the direction perpendicular to the plane where the cover frame is located through the pressing units. The locking mechanism is clamped into the groove to clamp the cover turning mechanism on the cabin body, and the cover turning mechanism is located above the chip placing seat. The chip synthesis device is high in function integration, and multiple functions such as chip cleaning, public reagent supply, reagent replacement, reagent cleaning, chip blowing and chip drying required in the chip synthesis process are achieved.

The invention discloses a singing play cavity synthesis method, relates to the field of singing play cavity synthesis, solves the problems that data is deficient due to the lack of annotation data in existing singing play synthesis, and local feature modeling of data by a voice synthesis model and a singing sound synthesis model is insufficient, and provides the following scheme. The method comprises the following steps of: sampling hidden representation similar to the output characteristics of an encoder from a codebook of EnCodec by utilizing the strong capability of the neural audio codec EnCodec, and reconstructing the hidden representation into audio, so that a song play data set is successfully expanded, and more data support is provided for model training; according to the method, the FG-GAN model is proposed, a GConformer module is proposed, and a grouping depth separable convolution module is proposed on the basis of a Conformer architecture, so that the receptive field of the model to a spectrogram is expanded, and the capture capability of the model to local features is enhanced. In addition, the FG-GAN also provides a frame-level implicit representation modeling module based on a stream model, so as to better simulate the change of audio in a phoneme or pitch.

The application discloses a live broadcast background fusion method and system, a storage medium and a live broadcast device, which are used for realizing higher quality of synthesis effect and enhancing the reality and immersion of live broadcast content. The method comprises the following steps: acquiring a recording and broadcasting material, and performing image segmentation on the recording and broadcasting material to obtain a foreground object and a background image in the recording and broadcasting material; performing image understanding on the foreground object, and converting the foreground object into a first 3D point cloud according to the result of image understanding; acquiring a real-time portrait, and acquiring a second 3D point cloud of the real-time portrait; and synthesizing a target live broadcast image according to the first 3D point cloud, the second 3D point cloud and the background image.

The invention discloses an MRI (Magnetic Resonance Imaging)-PET (Positron Emission Tomography) medical image synthesis method and device, and the method comprises the steps: constructing a forward modal attribute decoupling mechanism: decoupling a corresponding appearance vector and an anatomical structure vector from an MRI modal image and a PET modal image, and synthesizing the PET modal image through the appearance vector of the PET modal image and the anatomical structure vector of the MRI modal image; constructing a backward modal attribute decoupling mechanism: learning distribution of appearance vectors of PET modal images by using Gaussian vectors, and guiding MRI to PET medical image synthesis by using the Gaussian vectors as the appearance vectors of the PET modal images in a test stage; and designing a diagnosis-oriented modal attribute optimization strategy: predicting more accurate diagnosis information by effectively polymerizing complementary disease-related information between the MRI and the PET modal image so as to guide the network to synthesize a high-quality PET modal image. The device comprises a processor and a memory. According to the method, modal attribute information contained in different imaging modes is fully mined to guide the network to synthesize a high-quality PET modal image.

The invention relates to the field of video image synthesis, and particularly discloses a video image intelligent synthesis method based on artificial intelligence, which comprises the following steps: acquiring video image data to be synthesized and preprocessing; according to the data size and the diversification degree of the to-be-synthesized video image data, the target and the demand of the video image synthesis task, and the attribute of each artificial intelligence model of the video image synthesis, the adaptive artificial intelligence model of the video image synthesis is screened, and the synthesis effect of the video image is improved; basic parameters of video image synthesis are further set, indexes such as a peak signal-to-noise ratio, a structural similarity index, a feature similarity index and information entropy of video image synthesis are detected, the effect of video image synthesis is evaluated, whether video image synthesis needs to be optimized or not is judged, corresponding processing is carried out, and then intelligent synthesis of video images is achieved. The efficiency of video image synthesis is improved, and the quality of video image synthesis is guaranteed.

The present application discloses a cross-language speech synthesis method, device, electronic device and storage medium, the method comprising: obtaining speech data of a target object and speech data of an object to be synthesized; wherein the speech data of the target object is in a first language; the speech data of the object to be synthesized is in a second language; the second language and the first language are different languages; obtaining a timbre feature matrix of the target object; and encoding the phonemes, pitches and phoneme durations of the speech data of the object to be synthesized, and adding a set note at the phonemes with the same pronunciation or fuzzy pronunciation to obtain processed encoded data; decoding the timbre feature matrix and the encoded data to obtain synthesized speech data based on the second language with the timbre feature matrix. The present application can make the synthesized speech data have the timbre of the target object while retaining the pronunciation habits of the second language, so as to improve the transferability of the language, and then improve the cross-language speech synthesis effect.

The invention relates to the field of cosmetics, in particular to a composition with a function of activating endogenous synthesis of hyaluronic acid in skin cells. The composition can activate endogenesis of skin cells to synthesize hyaluronic acid, and comprises 0.05%-3% (w / w) of a hyaluronic acid synthesis inducing component and 0.0001%-2.5% (w / w) of a mitochondrial autophagy activating component, wherein the hyaluronic acid synthesis inducing component comprises hydroxypropyl tetrahydropyrantriol and / or sodium polyglutamate, and the mitochondrial autophagy activating composition is selected from one or more than two of nicotinamide, nicotinamide adenine dinucleotide, carnosine and hexapeptide-11. The composition can efficiently promote endogenous synthesis and secretion of hyaluronic acid in skin cells, the absorption process of exogenous hyaluronic acid is omitted, and the problem that the absorptivity of the exogenous hyaluronic acid is low is solved.

The invention belongs to the technical field of pharmaceutical preparations, and discloses a collagen regeneration preparation. Comprising SRT1720 and auxiliary materials. The product can promote skin fibroblasts to synthesize more endogenous I-type and III-type collagen, increase the skin compactness and quickly improve the facial appearance.

This invention relates to a composition for repairing the lipid barrier and its application in sensitive skin care, belonging to the field of cosmetic technology. The composition includes *Plasmodium purpureus* extract, flaxseed extract, senna-based glycosides, and oat extract. The rational combination of these four components not only promotes lipid synthesis but also ensures effective lipid transport and prevents the oxidative degradation of newly synthesized lipids. By covering the entire lipid metabolism process, it achieves a long-lasting and stable barrier repair effect, and can be applied to the repair of the lipid barrier in people with sensitive skin. Efficacy testing shows that the *Plasmodium purpureus* extract, flaxseed extract, senna-based glycosides, and oat extract in the composition can synergistically enhance the composition's antioxidant and lipid synthesis-promoting capabilities, while effectively promoting skin barrier repair and increasing skin moisture content, exhibiting significant skin-soothing and repairing effects, making it highly suitable for people with sensitive skin.

The invention relates to a genetic engineering strain, a preparation method thereof and an application in cell-free protein synthesis, the genetic engineering strain is provided, the expression or activity of a PAT1 gene in the strain is reduced, the modified strain is used for an in-vitro cell-free synthesis system, and verification shows that the PAT1 gene in the strain can be converted into the PAT1 gene in the cell-free synthesis system. The cell-free synthesis system using the strain can significantly improve the in-vitro cell-free protein synthesis ability.

The present application relates to a method, device, equipment and storage medium for improving the drugability of a compound molecule. The method: obtain a lead compound molecule, slice the lead compound molecule according to a preset slicing rule, obtain all chemical environments and replaceable molecular fragment combinations corresponding to the lead compound molecule, match the corresponding substitution rules in the matching molecule pair database based on the chemical environment, replace the molecular fragments according to the substitution rules, and generate multiple candidate compound molecules, wherein the matching molecule pair database contains multiple substitution rules, each substitution rule is about the change direction of ADMET drugability, and multiple candidate compound molecules are screened according to the preset rules to obtain multiple target compound molecules. Since the substitution rules contained in the matching molecule pair database are about the change direction of ADMET drugability, it is possible to imitate the design ideas of pharmaceutical chemists and improve and optimize the drugability of compound molecules.