292results about How to "Improve synthesis ability" patented technology

The invention relates to an injectable crosslinked hyaluronic acid gel and a preparation method thereof. The injectable crosslinked hyaluronic acid gel is prepared by blending hyaluronic acid gel granules and chlorinated sodium phosphate physiological buffer solution; the hyaluronic acid gel granules are prepared by comprising the steps of crosslinking treatment, emulsion crosslinking granulation, purification and drying and swelling, filling and sterilization technologies. The hyaluronic acid gel granules are uniform in granule size, the residual of the crosslinking agent is less than 0.2ppm, the injection pushing is proper, and the in-vivo degradation time can last more than 8-12 months. The implant has an excellent esthetical restoration effect, is applicable to being injected to and subcutaneous dermis deep layer to subcutaneous superficial layer, restoration of moderate to severe wrinkles or folds, and can satisfy the restoration demand of wrinkles or folds caused due to skin aging.

The invention relates to a unit selection speech synthesis method based on an acoustic statistical model. The method consists of an extraction of each acoustic feature in training corpus, a training of the statistical model that is corresponding to all kinds of acoustic features combining with the symbol information as syllabic segment and rhythm of each sentence in training corpus and the requirement of statistical model of each acoustic feature corresponding to each phoneme in awaiting synthesis sentences through the text analysis of input text when synthesizes. Based on the norm of maximumlikelihood value between acoustic parameters in awaiting selection unit sequence and acoustic statistical model of awaiting synthesis sentences, the best awaiting selection unit is searched. A KLD between acoustic statistical models is used for achieving fast beforehand selection of synthetic units. Finally, the synthetic speech of a sentence is acquired through smoothing and splicing of best awaiting selection unit waveform of each phoneme.The invention improves the quality of synthetic speech and synthetic effect of traditional splicing and synthetic method and achieves an automatic establishment of the system and an independence of the language kind.

Synthesis of the second strand of cDNA using pol ∝-primase is provided. Pol ∝-primase is used to initiate primers on a cDNA strand de novo, followed by extension of the primer with pol ∝-primase, a second DNA polymerase or a combination thereof. In a preferred embodiment, pol ∝-primase extends the primer, followed by addition of a second DNA polymerase, preferably Klenow fragment.

The invention discloses a method for positioning and tracking eye corners and mouth corners of a human face and a device thereof. In the invention, firstly a human face detection algorithm is adopted to obtain a position of the human face; an AAM algorithm is adopted to obtain an affined transformation coefficient for the detected human face and preliminary positions of six corner points of the eyes and the mouth on the human face; AdaBoost training models of all the corner points are combined to search the positions of candidate points in a neighborhood, so as to obtain a certain number of candidate points for each corner point; Harris corner point features of all the corner points are calculated, and the number of the candidate points of all the corner points are reduced according to certain rules; the candidate points of the corner points of the left eye, the right eye and the mouth are respectively combined into pairs; the point pairs are gradually eliminated by adopting a plurality of features, and finally an optimum result is returned. The proposal provided by the embodiment of the invention solves the problem of inaccurate positioning of the corner points of the eyes and the mouth of the human face in all kinds of gestures, and realizes the positioning of outer profiles of the eyes and the mouth of the human face, thereby providing a feasible scheme for driving human face two-dimensional and three-dimensional models.

The embodiment of the application relates to an image processing method and device, electronic equipment and a computer readable storage medium. The above method includes the following steps: acquiring at least two frames of first-type images acquired by a first camera, wherein the exposure time of the at least two frames of first-type images is the same; performing registration on the at least two frames of first-type images to obtain alignment parameters; acquiring at least two frames of second-type images acquired by a second camera, wherein the exposure time of the at least two frames of second-type images is different; performing registration on the at least two frames of second-type images according to the alignment parameters; and synthesizing the registered second-type images to generate a high dynamic range image. According to the image processing method and device, the electronic equipment and the computer readable storage medium, the accuracy of image registration can be improved, and the synthesis effect of HDR images can be improved.

The invention provides a rhythm-level prediction model generation method and apparatus, and a rhythm-level prediction method and apparatus. The rhythm-level prediction model generation method comprises: generating an initial rhythm-level prediction model according to a precisely marking data set; collecting speech and corresponding text data, detecting feature information in the speech, and adding the feature information to a corresponding position of the text data; according to the initial rhythm-level prediction model, carrying out rhythm-level prediction on the text data with added feature information to obtain an initial rhythm-level prediction result; and according to the precisely marking data set and the initial rhythm-level prediction result, carrying out training to generate an updated rhythm-level prediction model, and applying the updated rhythm-level prediction model to rhythm-level prediction for speech synthesis. With the method, accuracy of the generated rhythm-level prediction model is improved and thus accuracy of the rhythm-level prediction is enhanced; and the voice synthesis effect is improved.

The invention relates to a gene, mutant plasmid and engineering bacteria which have improved synthesis performance to penicillin G acylase and are obtained by a gene site-directed mutagenesis method, and mutant enzyme can also be obtained with improved synthesis performance to penicillin G acylase by fermenting and purifying the engineering bacteria. Two enzymes Kpn I and Pst I are firstly used for cutting pUC18 by the invention, then T4 polymerase is adopted to make the ends blunt, and pZ01 is obtained through self-linkage; the enzyme of EcoR I is used for cutting pZ01, and then connected with pEES102 that is also cut by the enzyme of EcoR I, thereby obtaining the recombinant plasmid pY020; the pY020 is adopted as a template plasmid, and TaKaRa MuTanBEST Kit is utilized for conducting the site-directed mutagenesis to B.megaterium PGA, thereby obtaining the mutant plasmid with improved synthesis performance to the penicillin G acylase. The mutant plasmid is transformed to bacillus subtilis to obtain the required engineering bacteria. The engineering bacteria are amplified and fermented, and the mutant enzyme with improved maximum conversion rate of 7-ADCA and the ratio of synthetic product / hydrolysate can be obtained after the engineering bacteria are purified.

The invention discloses an optimized in vitro cell-free protein synthesis system. The system comprises a cell extract, a carbohydrate material, a phosphate compound, a buffering agent and a DNA molecular template for encoding an exogenous protein, wherein the cell extract is a yeast cell extract inserted into a T7 RNA polymerase gene; the carbohydrate material is a mixture of glucose and maltodextrin; the buffering agent is a trihydroxymethyl aminomethane buffering agent; and the DNA molecule template is prepared by a nucleic acid isothermal amplification method, and a sequence as shown in SEQID NO.1 is inserted into the upstream of the coding sequence of the exogenous protein in the DNA molecular template. By optimizing, the cost of in vitro protein synthesis is reduced and the yield ofthe target protein is increased.

The invention discloses an image processing method and apparatus, an electronic device and a storage medium. The method comprises the steps of performing feature extraction processing on a to-be-processed image to obtain feature data of a first target object in the to-be-processed image; and according to the feature data of the first target object, performing image synthesis to obtain a first synthesized image. According to the image processing method and apparatus, the feature data of the target object in the to-be-processed image is extracted, and the synthesized image is obtained accordingto the feature data, so that the image synthesis effect is improved.

Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and / or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.

The invention provides a method for preparing a seaweed fermentation solution by virtue of probiotics fermentation and application of the seaweed fermentation solution in preparing cosmetics, which belongs to the technical field of microbial fermentation of seaweeds. The method comprises the following steps: (1) superfine grinding seaweeds, and obtaining seaweed powder with the granularity of 1 to100 micrometers; (2) mixing the seaweed powder and a bacillus fermentation solution, performing the primary fermentation, and obtaining a primary seaweed fermentation solution; (3) inoculating saccharomycetes into the primary seaweed fermentation solution, performing the secondary fermentation, and obtaining a secondary seaweed fermentation solution; (4) inoculating lactobacillus into the secondary seaweed fermentation solution, performing the tertiary fermentation, and obtaining a tertiary seaweed fermentation solution; and (5) standing the tertiary seaweed fermentation solution at 8 to 12 DEG C, fermenting for 15 to 60 days, separating solid and liquid, wherein the liquid component is the seaweed fermentation solution. The method is high in safety, pollution-free, good in skincare effect and high in stability.

The invention belongs to the skin care field and in particular relates to a skin care product which is added with medicinal plant extract and keratin. The skin care product is made from the components with the following weight percentage of 1-20% of the medicinal plant extract, 0.2-10% of the keratin, 5-15% of esters, 5-11% of oils, 1-4% of high fatty alcohol, 2-8% of emulsifier, 0.1-0.4% of carbomer, 0.1-0.4% of triethanolamine (99%), 0.1-1.0% of preservative and 0.1-1.0% of essence, and the rest is water. The medicinal plant extract added in the skin care product has the efficacies of promoting blood circulation, removing blood stasis, benefiting vital energy and strengthening essence, delays skin aging process, increases the skin smoothness and elasticity and has good antibacterial and antiphlogistic functions. The added keratin can promote the cell enlargement and the collagen synthesis, reduce skin fine cracks and wrinkles, keep skin moisture and strengthen the skin compactness and elasticity.

The invention relates to the technical field of biology, and discloses a coenzyme-Q10-production engineered bacteria construction method, engineered bacteria, and an application thereof. The method comprises the steps that: a, total genomic DNA is extracted from Rhodobacter sphaeroides cacterial liquid; b, bchG homologous gene is obtained by amplification with a polymerase chain reaction; c, the amplified homologous gene is connected with broad-host plasmid, such that recombinant vector is constructed; d, the recombinant vector is transferred to Escherichia coli S17-1; and e, the Escherichia coli S17-1 is subjected to conjugal transfer with the Rhodobacter sphaeroides, such that the engineered bacteria with chlorophyll synthesis gene bchG knocked out are obtained. According to the method provided by the invention for improving coenzyme Q10 yield through knocking out Rhodobacter sphaeroides chlorophyll synthesis gene, coenzyme Q10 synthesis capacity can be improved by higher than approximately 15%. The method is suitable for coenzyme Q10 large-scale industrial production.

The invention discloses a method for producing glutathione by fermentation of recombinant Escherichia coli. The method comprises the following stages of: (1) carrying out batch culture or fed-batch culture to obtain a great deal of recombinant Escherichia coli cells expressing exogenous bifunctional glutathione synthetase, wherein the bifunctional glutathione synthetase is protein which simultaneously has activities of gamma-glutamyl cysteine synthetase and glutathione synthetase; (2) carrying out over-expression on the bifunctional glutathione synthetase obtained in the stage (1); and (3) adding L-glutamic acid, L-cysteine and glycine precursor amino acids to realize high-efficiency production of glutathione by fermentation. According to the invention, the recombinant Escherichia coli strain expressing the exogenous bifunctional glutathione synthetase is used for producing glutathione by fermentation, so that the method not only has high GSH (Glutathione) synthesizing capability, but also is free from GSH feedback inhibition, and has the advantages of rapid reaction, high glutathione yield and short production period.

The invention discloses a single shot mixed reality realization method combining environment mapping and global illumination rendering. The method comprises the steps of positioning a virtual object and generating a realistic scene model; voxelizing and filtering the real scene model and virtual object; generating an environment map, and segmenting the realistic scene image by uniform distribution, and then inverting and mapping the image to form the environment map; carrying out cone-tracking rendering, and using the environment mapping of the cone-tracking rendering output to become a composite image. The method can utilize the powerful graphics computing power of the GPU to enhance the output effect of a general mixed reality system, and can simulate the reflection effect of a bright object on the surrounding environment under the condition of a single lens.

The invention provides a human and scene image synthesis method based on rotary camera lens photographing. The method includes the steps that a rotary camera lens is set to be in a front preview state, a self-photographing image is obtained in the preview state and a figure image is extracted; the rotary camera lens is set to be in a rear photographing state and photographs a scene image; the figure image and the scene image are synthesized to obtain a human and scene synthesis image. The invention further provides a human and scene image synthesis system based on rotary camera lens photographing. Due to the fact that the synthesized images are photographed through the same camera lens, image resolutions are the same, so that synthesis efficiency can be improved, the phenomenon that the human and scene synthesis image is distorted after figures are processed is avoided, and the synthesis effect is improved.

The invention provides a 3D size measuring method. The method comprises the following steps of acquiring information of multiple images of a first target at different angles; building a first point cloud 3D model of the first target according to the information of the multiple images; and comparing the first point cloud 3D model with point cloud 3D model samples bound with identity information andpre-stored in a first sample database one by one, finding the point cloud 3D model sample matched with the first point cloud 3D model, and measuring the geometric difference between the first point cloud 3D model and the point cloud 3D model sample, wherein the three images in the multiple images at least have parts representing the same region of a target object, and a point cloud is a sparse point cloud or a dense point cloud. It is proposed that the sparse or dense point cloud of the target object and the sample are used for performing measurement / comparison, so that the measurement / comparison speed is increased. It is creatively proposed that the picture quality influences the 3D measurement / comparison precision and speed. By means of optimizing a relation between adjacent shot pictures, the obtained picture is guaranteed to be high in synthesis precision.

The invention provides a preparation method of strong aromatic Chinese spirits pit mud by adding red yeast esterified enzyme, and relates to the technical field of spirits making. The preparation method comprises the following steps of: adding 10kg of red yeast esterified enzyme and 10kg of yeast powder into mixed infiltrating body of 35kg of yellow clay, 3kg of old pit mud, 20kg of mud carbon, 5kg of bean cake powder, 5kg of double-wheel bottom flavour grains, 0.35kg of silkworm chrysalis powder, 2kg of yellow water, 10kg of hot vinasse, 10kg of 20 to 22-degree cocktail and 5kg of nutrient solution which respectively can pass a screen mesh of 10 and have the pH value of 5.5-6.0; vaccinating 10kg of compound hexanoic acid bacteria-enriched liquid, stirring and mixing to prepare pit mud grains; sealing the periphery of the pit mud grains by a plastic film; and cultivating for 30-35 days under the anaerobic condition at the temperature of 33-35 DEG C, and detecting the components and the beneficial microbe bacterial counts of the pit mud to obtain the strong aromatic Chinese spirits pit mud by adding the red yeast esterified enzyme. The preparation method is used for cultivating the pit mud in a strong aromatic Chinese spirits preparation technology. The preparation highlights a nitrogen source, trace element and the red yeast esterified enzyme which influences aroma-producing substances and flavor-producing substances of the Chinese spirits.

The embodiment of the invention discloses an image data processing method and device; the method comprises the following steps: obtaining user image characteristics matched with a user image and sent by a server; obtaining to-be-filled image characteristics matched with a to-be-filled area in a preset image, and searching a display image area matched with the to-be-filled image characteristics from the user image according to the user image characteristics; adding the image data of the display image area to the to-be-filled area, and synthesizing the image data of the display image area with the preset image, thus obtaining a synthesis image. The image data processing method can prevent artificial synthesis errors, thus improving the synthesis image synthesis effect.

The present invention discloses an in-vitro cell-free protein synthesis system. The system comprises cell extract, carbohydrate and a phosphate compound, wherein the carbohydrate is maltodextrin, lactose, or a combination of the maltodextrin and glucose, or a combination of the lactose and the glucose, or a combination of the maltodextrin, the lactose and the glucose. ATP is provided for an in-vitro reaction by low-cost substances such as the glucose, the maltodextrin and the lactose instead of energy sources such as phosphoenolpyruvic acid, phosphocreatine and acetyl phosphate, so that whilethe cost is reduced, a mode of providing energy by slow release prolongs the reaction time and increases the yield of target protein.

The invention provides a preparation method of 2-(2', 2'-difluoroethoxyl)-6-trifluoromethyl phenyl propyl sulfide, having the following processes: reacting m-trifluoromethyl phenol with p-toluenesulfonyl chloride, trifluoromethanesulfonic anhydride or 3, 5-dinitrobenzol sulfonyl chloride in a solvent containing organic base or a solvent containing triethylamine hydrochloride and organic base, andthen, collecting a compound having a formula (III) from reaction products, dissolving the compound in butylene oxide, adding tetramethyl ethylene diamine and diisopropylamine, at a temperature range from -25 DEG C to -100 DEG C, adding butyl lithium cyclohexane solution for reaction, and then, adding dipropyldisulfide, and collecting a compound having a formula (II); reacting the compound having the formula (II) with 4-nitrogen, nitrogen-dimethyl pyridine and difluoroethanol for catalytic reaction in the solvent, and then, collecting a compound having a formula (I) from the reaction products.The invention simplifies reaction conditions, optimizes the technology, reduces production cost, and improves synthesis effects. The general formula of the reaction is as follows.

The invention discloses a genetic engineering bacterium for high-yield phloroglucinol as well as a construction method and an application of the genetic engineering bacterium and belongs to the technical field of genetic engineering. The genetic engineering bacterium over-expresses carbon storage and regulation factor gene CsrB shown by SEQ ID NO.1 and co-expresses a polyketide synthase gene phlD, a multiple resistance activating factor gene marA and acetyl CoA carboxylase gene ACCase. The invention also provides the construction method and the application of the genetic engineering bacterium for the high-yield phloroglucinol. With the adoption of the genetic engineering bacterium, the yield of the phloroglucinol is firstly increased by 115.6 percent by adopting a mode of globally regulating carbon metabolism after post-transcriptional level of the genetic engineering bacterium, and the genetic engineering bacterium has a high industrial application value.

The invention discloses an epsilon-polylysine fed batch fermentation method for enhancing the cell growth and the bioprocess efficiency. The method comprises the following steps: carrying out fermenting culture of epsilon-polylysine producing strains by a fermenting culture medium, supplementing 0.0.25g-0.5g of yeast powder or other organic nitrogen sources to each liter of the fermenting culture medium in a culture system each hour when the pH value of the culture system reduces to 3.6-4.1 until fermentation ending, maintaining the pH value of the culture system in a range of 3.6-4.1 by ammonia water until fermentation ending, supplementing a disinfected fed culture medium containing glucose and ammonium sulfate, and maintaining the content of glucose in the culture system in a range of 5g / L-30g / L until fermentation ending. The fast entrance of cells to the epsilon-polylysine synthesis phase avoids the cell activity loss before the cell synthesis phase and prolongs the cell epsilon-polylysine synthesis time, so the cell epsilon-polylysine synthesis capacity is substantially improved.

The invention discloses a strain for generating 3-hydroxy butanone and the application thereof, which belong to the field of bioengineering technology. The stain is named as Paenibacillus polymyxa ME-J25 and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.2505. The strain can be fermented to generate 3-hydroxy butanone with higher concentration, higher transformation efficiency and extremely low content of the reduced byproduct 2,3-butanediol of 3-hydroxy butanone, and is suitable for industrial production of 3-hydroxy butanone.

Described herein is a method of cloning synthetic oligos (including in situ synthesized oligos) into an (one or more) expression vector for library (e.g., shRNA library) production. The oligos are synthesized with one portion of the first stem of the hairpin, followed by a first loop sequence, the complete second stem, a second loop sequence, and finished with the remaining portion of the first stem of the hairpin. The two portions of the first stem anneal to the second stem, juxtaposing the 5′ end close to the 3′ end of the oligo. The methods described herein selected for hairpins with perfectly base-paired stems. After annealing, a ligase is added to the annealed oligos and the base-paired hairpins are preferentially annealed, and ligated, creating closed circular oligos. The now circularized hairpins served as templates for rolling circle amplification using a polymerase with high processivity. One or more primers complementary to the two strands of the amplified double stranded circular hairpins initiate the rolling circle amplification in the presence of a polymerase. Using primers (e.g., a sense and antisense primer), the rolling circle amplification yields double stranded hairpin sequences. These can be digested (e.g., using restriction enzymes) to produce a double-stranded hairpin fragment encoding a single hairpin. The fragment can be cloned into an appropriately digested vector for a variety of uses including expression.

The invention discloses a Bacillus alcalophilus SD 7903 with the preservation number of CGMCC No. 3158, belonging to the field of microbial technology; the invention also discloses a culture method thereof and an application in producing Ectoine. The Bacillus alcalophilus has higher Ectoine synthesis capability and is a strain of Gram positive bacteria, thus expanding biological resource utilization space and having good industrial application prospect in respect to production of Ectoine mainly by applying Gram negative bacteria in the current industry.

The invention provides a gene modification method of n eukaryotes. In the genome of a eukaryote, an eIF2B delta gene is fused with a T7 RNA polymerase gene and / or an eIF2B epsilon gene is fused with the T7 RNA polymerase gene through a gene editing technology. Tests prove that the protein expression capacity of a cell-free system can be remarkably improved by respectively fusing the T7 RNA polymerase with two subunits, namely eIF2B delta and eIF2B epsilon of an eIF2B complex. Meanwhile, the invention provides a genetically engineered cell modified by using the gene modification method, and aneukaryotic acellular protein synthesis system containing a cell extract prepared from the genetically engineered cell.

The invention discloses a file combining method and a file combining device. The method includes the steps: reading one or multiple to-be-converted files in a to-be-converted file directory of current equipment; dividing all the to-be-converted files into one or multiple to-be-converted file group according the file converting number, wherein the file converting number is determined according to performance parameters of the current equipment; sequentially performing format conversion of all the to-be-converted files in each to-be-converted file group, and combining format conversion results into a target format file. By the aid of the method, the files of various formats can be converted and combined by computer equipment, errors are difficult to occur, and format conversion efficiency and combining performance are improved.

The invention discloses a method for efficiently synthesizing PHB by knocking out flagellum and pilus genes, which belongs to the field of gene engineering and fermentation engineering. According to the invention, three flagellum gene clusters flhE-motA, fliY-fliR and flgN-flgL on an escherichia coli genome are knocked out to obtain a flagellum simplified strain WJW010, and a fimbriae gene clusterfimB-fimH is knocked out to obtain a fimbriae simplified strain WJW011, the three genes synthesized by PHB can be transformed into strains WJW010 and WJW011, and the recombinant bacteria WJW010 / pBHR68 and the recombinant bacteria WJW011 / pBHR68 are obtained; pHB with the cell dry weight of 19.2% and 2.8% can be synthesized under the normal fermentation condition, and the volume yield of PHB is 9.6times and 1.4 times that of wild control bacteria W3110 / pBHR68 (2%).