Method for preparing seaweed fermentation solution by virtue of probiotics fermentation and application of seaweed fermentation solution in cosmetics

A technology of fermented liquid and probiotics, which is applied to the preparation of seaweed fermented liquid by probiotics fermentation and the application field of cosmetics, which can solve the problems of environmental pollution damage, low added value of products, and low technical content, so as to reduce pollution and promote collagen production. Protein synthesis ability, effect of increasing the type and content of active ingredients

Active Publication Date: 2018-10-16
山东福瑞达生物股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the development of kelp in my country is currently mainly focused on the extraction of kelp food, seaweed (belt) fertilizers, and chemical raw materials such as iodine, alginic acid, and mannitol. Deep processing of seaweed, efficient and comprehensive utilization of active substances in seaweed
[0003] Seaweed has the advantages of pure growth environment, strong vitality, and high content of active ingredients. It has become the main functional raw material in the field of skin care products widely recognized in European and American countries. Many first-line skin care brands have developed seaweed element skin care products. Most of the extractions are based on acid and alkali methods. This method not only has a low extraction rate, but more importantly, the use of acid and alkali brings a series of waste treatment problems, which increases the cost and seriously damages the environment.

Method used

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  • Method for preparing seaweed fermentation solution by virtue of probiotics fermentation and application of seaweed fermentation solution in cosmetics
  • Method for preparing seaweed fermentation solution by virtue of probiotics fermentation and application of seaweed fermentation solution in cosmetics
  • Method for preparing seaweed fermentation solution by virtue of probiotics fermentation and application of seaweed fermentation solution in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Soak the dried kelp in pure water, take 1 part of kelp and 15 parts of water; after fully soaking the dried kelp, rinse it repeatedly with pure water to wash off the sediment and salt on the surface of the kelp;

[0043] 2) The clean kelp is dried at low temperature, and the particle size of the dried kelp ultrafine powder is less than 50 μm;

[0044] 3) Activating the bacterial strains used for fermentation, wherein the bacterial strains used for fermentation are Bacillus amyloliquefaciens and Bacillus licheniformis;

[0045] 4) sterilizing the kelp dried at low temperature by irradiation, and irradiating at 1000-2500 kilorads for 2 hours;

[0046] 5) The activated Bacillus amyloliquefaciens was cultured with a fermentation medium, and the medium formula was: 1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract. Bacillus was cultured until the number of viable cells reached 10 9 After cfu / mL, add the ultrafinely pulverized and sterilized...

Embodiment 2

[0056] 1) Soak the dried kelp in pure water, take 1 part of kelp and 10 parts of water; after fully soaking the dried kelp, rinse it repeatedly with pure water to wash off the sediment and salt on the surface of the kelp;

[0057] 2) The clean kelp is dried at low temperature, and the particle size of the dried kelp ultrafine powder is less than 70um;

[0058] 3) Activating the bacterial strains used for fermentation, wherein the bacterial strains used for fermentation are Bacillus natto; Rhodotorula viscose, Pichia powder; Lactobacillus gasseri, Bifidobacterium;

[0059] 4) sterilizing the kelp dried at low temperature by irradiation, and irradiating at 1000-2500 kilorads for 2 hours;

[0060] 5) Cultivate the activated Bacillus natto with a fermentation medium, and the medium formula is: 1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract. Bacillus natto was cultured until the number of viable cells reached 10 9 After cfu / mL, add the ultrafinely...

Embodiment 3

[0069] 1) Soak the dried kelp in pure water, take 1 part of kelp and 17 parts of water; after fully soaking the dried kelp, rinse it repeatedly with pure water to wash off the sediment and salt on the surface of the kelp;

[0070] 2) The clean kelp is dried at low temperature, and the particle size of the dried kelp ultrafine powder is less than 100um;

[0071] 3) Activating the bacterial strain used for fermentation, wherein the bacterial strain used for fermentation is Bacillus amyloliquefaciens; Candida utilis; Streptococcus thermophilus, Lactobacillus casei;

[0072] 4) sterilizing the kelp dried at low temperature by irradiation, and irradiating at 1000-2500 kilorads for 2 hours;

[0073] 5) The activated Bacillus amyloliquefaciens was cultured with a fermentation medium, and the medium formula was: 1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract. Bacillus amyloliquefaciens was cultured until the number of viable cells reached 10 9 After ...

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Abstract

The invention provides a method for preparing a seaweed fermentation solution by virtue of probiotics fermentation and application of the seaweed fermentation solution in preparing cosmetics, which belongs to the technical field of microbial fermentation of seaweeds. The method comprises the following steps: (1) superfine grinding seaweeds, and obtaining seaweed powder with the granularity of 1 to100 micrometers; (2) mixing the seaweed powder and a bacillus fermentation solution, performing the primary fermentation, and obtaining a primary seaweed fermentation solution; (3) inoculating saccharomycetes into the primary seaweed fermentation solution, performing the secondary fermentation, and obtaining a secondary seaweed fermentation solution; (4) inoculating lactobacillus into the secondary seaweed fermentation solution, performing the tertiary fermentation, and obtaining a tertiary seaweed fermentation solution; and (5) standing the tertiary seaweed fermentation solution at 8 to 12 DEG C, fermenting for 15 to 60 days, separating solid and liquid, wherein the liquid component is the seaweed fermentation solution. The method is high in safety, pollution-free, good in skincare effect and high in stability.

Description

technical field [0001] The invention belongs to the technical field of microbial fermented seaweed, and in particular relates to a method for preparing seaweed fermentation liquid by probiotic fermentation and an application of the seaweed fermentation liquid in preparing cosmetics. Background technique [0002] There are many kinds of seaweed and abundant resources, which are important marine resources. In my country, seaweed is mainly kelp. Kelp, also known as kombu and lunbu, are often used as raw materials for food and traditional Chinese medicine. Rich in physiologically active substances such as unsaturated fatty acid, fucoxanthin and sterol compounds. However, the development of kelp in my country is currently mainly focused on the extraction of kelp food, seaweed (belt) fertilizers, and chemical raw materials such as iodine, alginic acid, and mannitol. Deep processing of seaweed, efficient and comprehensive utilization of active substances in seaweed. [0003] Sea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9711A61K8/9706A61Q19/08
CPCA61K8/9706A61K8/9711A61K2800/85A61Q19/08
Inventor 王婷赵林李昆仑梁艳岳秋林
Owner 山东福瑞达生物股份有限公司
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