902 results about "Bacillus amyloliquefaciens" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention provides a southern red soil dry land deacidification soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The preparation method comprises the following steps: embedding bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens to prepare bacterial microspheres, adding the bacterial microspheres into gel formed by carboxymethyl chitosan and silk fibroin peptide to prepare sustained-release bacterial microsphere gel, uniformly mixing the sustained-release bacterial microsphere gel with porous silicon dioxide loaded with basalt and charcoal, and drying to prepare the acid-reducing soil conditioner for the southern red soil dry land. The acid-reducing soil conditioner for the southern red soil dry land not only improves the pH value of the soil, but also can loosen the soil and improve the air permeability of the soil to a certain extent, and promotes the absorption of crops to moisture and nutrient elements by improving the physical and chemical properties of the soil, so that the normal growth of the crops is ensured, and the acid-reducing soil conditioner has a wide application prospect.

The invention relates to bacillus amyloliquefaciens, a biocontrol inoculant prepared from the bacillus amyloliquefaciens and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens and is named as JK2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CMCC No.31376, and the preservation time is July 19, 2024. The bacillus amyloliquefaciens JK2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus amyloliquefaciens JK2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention provides bacillus amyloliquefaciens LYZ1125, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC (China General Microbiological Culture Collection Center) No.31716, and the preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC No.31716. The bacillus amyloliquefaciens LYZ1125 disclosed by the invention has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same species and different species as the verticillium medicago, and pot experiment shows that the bacillus amyloliquefaciens LYZ1125 can promote the growth of alfalfa root systems and plants; the morbidity of the alfalfa verticillium wilt can be remarkably reduced, the good prevention and treatment effect on the alfalfa verticillium wilt is achieved, and an important theoretical basis is provided for biological prevention and treatment of the alfalfa verticillium wilt (V.alfalfae) LYZ0257).

The invention discloses a functional microbial organic fertilizer for saline-alkali soil improvement and a preparation method and application thereof, and belongs to the technical field of fertilizers. In order to solve the technical problem that high salt ion content in saline-alkali soil causes multiple adverse effects on plant growth, the invention provides a functional microbial organic fertilizer for saline-alkali soil improvement. The functional microbial agent is prepared from bacillus amyloliquefaciens, insecticidal cupriavidus, trichoderma harzianum, halophilic bacillus grignard and halotolerant bacillus, and the functional microbial carrier organic fertilizer is added with furfural residues and citric acid residues. The functional microbial organic fertilizer provided by the invention has the functions of improving the microbial abundance of saline-alkali soil, improving the physicochemical properties of the soil, adjusting the pH value of the soil, increasing the proportion of soil aggregates and increasing the contents of rapidly available phosphorus and rapidly available potassium. The functional microbial organic fertilizer provided by the invention can be applied to effectively improving saline-alkali soil.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention discloses bacillus amyloliquefaciens GX0003936 and application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus amyloliquefaciens GX0003936 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center on March 26, 2025, and the preservation number of the bacillus amyloliquefaciens GX0003936 is CGMCC (China General Microbiological Culture Collection Center) No.33987. The bacterial strain has broad-spectrum antibacterial activity, can be used for remarkably inhibiting the growth of mycelia of colletotrichum musae and colletotrichum frutescens, and can be used for remarkably reducing the disease incidence rate of tropical fruits such as bananas, mangoes and the like in the postharvest storage period. Meanwhile, the strain shows a good inhibition effect on various plant pathogenic fungi, and has a potential broad-spectrum biocontrol effect.

The invention relates to the technical field of feeds, and particularly discloses a cattle fattening feed which is technically characterized by comprising the following components in percentage by weight: 20-60 parts of a fermented mixture, 10-16 parts of modified coconut fiber, 0.5-1.5 parts of an additive and 5-10 parts of an additive, the fermented material mixture is prepared from soybean meal, corn, rice bran, pennisetum purpureum, hops, an aspergillus oryzae culture, saccharomycetes, bacillus subtilis and bacillus amyloliquefaciens; the mass ratio of the soybean meal to the corn to the rice bran to the pennisetum purpureum to the hops is (2-4): (1-3): (3-5): (2-4): (0.4-0.9); the additive is fructus momordicae residues; the additive is a mixture of codonopsis pilosula and rhizoma atractylodis. The feed disclosed by the invention contains the siraitia grosvenorii residues, so that the apparent digestibility, the growth performance, the serum biochemical index and the antioxidant index of the fattening cattle can be improved, and the quality of the fattening cattle can be effectively improved.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

Methods are provided for enhancing the health, growth and / or sturdiness of turf grasses by applying a combination of microbes and / or their growth by-products to the turf grass roots and / or to soil. Specifically, in one embodiment, the subject invention utilizes Trichoderma harzianum and a strain of Bacillus amyloliquefaciens. The methods can further be used to improve maintenance and upkeep of natural turf grass fields used in, for example, athletics and recreation, as well as to increase the functionality and safety of these natural turf grass fields.

The invention relates to bacillus amyloliquefaciens which is classified and named as bacillus amyloliquefaciens LDB1, the preservation number of the bacillus amyloliquefaciens is CCTCC No: M 20231897, and the preservation date is October 16, 2023. The invention relates to application of bacillus amyloliquefaciens in citrus waste compost. The invention relates to a microbial agent, which comprises bacillus amyloliquefaciens LDB1, bacillus amyloliquefaciens, bacillus subtilis and bacillus velezensis. The invention relates to application of a microbial agent in citrus waste compost. The method has the beneficial effects that lignin peroxidase, laccase and manganese peroxidase can be produced, good lignin degradation capability is achieved, degradation of lignocellulose in citrus waste compost can be effectively promoted by matching with other strains, the degradation rates of hemicellulose, cellulose and lignin are 33.8%, 36.5% and 38.5% respectively, and the method is suitable for industrial production. The strain and the microbial inoculum thereof have important application value in the aspect of recycling of high-lignin fiber organic wastes.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention provides bacillus amyloliquefaciens as well as a construction method and application thereof. Wherein the bacillus amyloliquefaciens is the bacillus amyloliquefaciens of which the stress response transcription factor glcR gene is knocked out, and the bacillus amyloliquefaciens is a bacillus amyloliquefaciens of which the stress response transcription factor glcR gene is knocked out; the nucleotide sequence of the glcR gene is as shown in SEQ ID NO: 1. By knocking out a stress response transcription factor glcR gene in bacillus amyloliquefaciens, the level of glutaminase expression by using the bacillus amyloliquefaciens can be enhanced, the enzyme activity of the glutaminase obtained by expression is relatively high, and the activity of the enzyme can be further improved by using the modified strain; powerful technical support is provided for commercialized production of glutaminase, and great economic benefits and application prospects are achieved.

The invention discloses a carbon dioxide injection and production well cementation cement paste and a preparation method and application thereof, the well cementation cement paste comprises the following components by weight: 95-105 parts of oil well cement, 44-48 parts of water, 4-6 parts of a polymer emulsion, 4-6 parts of a self-repairing material, 1.5-2.5 parts of a drag reducer, 0.5-1.5 parts of a fluid loss agent and 0.2-0.8 part of an antifoaming agent; wherein the polymer emulsion is an emulsion which is formed by taking methyl methacrylate, vinyl trimethoxy silane and propylene carbonate as monomers through polymerization; the self-repairing material is a microcapsule in which thiobacillus ferrooxidans thalli and bacillus amyloliquefaciens thalli are embedded. The well cementation cement paste keeps good strength and relatively low permeability in a carbon dioxide environment, is good in corrosion resistance and has self-repairing capability.

The present invention provides a biological seed treatment agent, which comprises: a microbial agent, which is selected from at least one of Bacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound. The biological seed treatment agent of the present invention has the advantages of increasing seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, reducing fertilizer usage, etc., and is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot and alfalfa damping-off, and has good application prospects.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention belongs to the technical field of microorganisms, and particularly relates to rhizobium jangularis LN-ZDJ-1, a complex microbial inoculant containing the rhizobium jangularis LN-ZDJ-2 and application of the rhizobium jangularis LN-ZDJ-1 and the complex microbial inoculant. The rhizobium jangularis LN-ZDJ-1 is preserved in the China Center for Type Culture Collection on July 22, 2024, and the preservation number of the rhizobium jangularis LN-ZDJ-1 is CCTCC (China Center for Type Culture Collection) NO: M20241652; the bacillus amyloliquefaciens LN-ZDJ-2 is preserved in the China Center for Type Culture Collection on July 22, 2024, and the preservation number of the bacillus amyloliquefaciens LN-ZDJ-2 is CCTCC (China Center for Type Culture Collection): M 20241651; the compound microbial agent provided by the invention not only can remarkably improve the growth and yield of the Achyranthes bidentata, but also can improve the nutrient absorption capacity of plants, further promotes the healthy growth and resistance of the plants by secreting active substances such as auxin and siderophores, and is beneficial to improving the quality of pasture.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.

The invention provides bacillus amyloliquefaciens capable of improving color and luster of soy sauce, inhibiting bacteria and enhancing aroma and application of the bacillus amyloliquefaciens, and belongs to the field of microorganisms, the bacillus amyloliquefaciens Z-WQ20 is preserved in China General Microbiological Culture Collection Center on June 18, 2024, the preservation number is CGMCC NO.30996, and the preservation number is CGMCC NO.30996. The bacillus amyloliquefaciens Z-WQ20 is preserved in the China General Microbiological Culture Collection Center on June 18, 2024. The preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the strain disclosed by the invention can inhibit bacteria, remove black, improve color and luster and increase ester in the soy sauce fermentation production process, has the efficacy of enhancing aroma, and has a good application prospect.

The invention discloses bacillus for preventing and treating anthracnose of camellia oleifera and application of the bacillus. The preservation number of the bacillus CH-1-7 provided by the invention is GDMCC (Glucose Digital Microorganism Center) No. 66219; the bacillus amyloliquefaciens has the capability of generating living-promoting substances, can generate organic phosphorus and siderophores, has the nitrogen fixation capability, can inhibit the growth of various pathogenic bacteria, especially colletotrichum gloeosporioides, can be used for preventing and treating camellia oleifera anthracnose, and has important application value in the field of plant protection.

The invention discloses bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in blocking of root infection of pear fire blight bacteria, the bacillus amyloliquefaciens is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.28054, and the preservation date is July 28, 2023. The bacillus amyloliquefaciens has the advantages that the bacillus amyloliquefaciens can be applied to prevention of root infection of pear fire blight bacteria; the bacillus amyloliquefaciens provided by the invention solves the problems of trunk morbidity and tree withering caused by pear fire blight self-root infection.

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

The invention discloses microbial combined bacteria and a method for improving fertility of newly-cultivated land. The method comprises the following steps: combining every two of bacillus amyloliquefaciens WK1, white rot fungi NDM3-2 and trichoderma helichum HJR7 to form three combined bacteria; the three combined bacteria are applied to soil containing straw and lentinus edodes residues respectively, and a pot experiment (rice and white clover crop rotation) for two years is carried out with no addition of any bacteria as a contrast; analyzing the physical and chemical properties of the soil and the microbiological indexes of the soil; and according to an analysis result, the combination of the white rot fungi NDM3-2 and the trichoderma helichum HJR7 is confirmed to be an ideal scheme for improving the fertility of the newly-cultivated land.

The invention relates to the technical field of biology, in particular to recombinant bacillus for heterologous synthesis of ovotransferrin as well as a construction method and application of the recombinant bacillus. According to the invention, bacillus (bacillus subtilis and bacillus amyloliquefaciens) is taken as a host, and the OVT gene is heterologously expressed by using a genetic engineering technology, so that heterologous secretory expression of OVT1 and OVT2 from different sources in chassis cells is realized; meanwhile, the invention also provides a method for more economically and efficiently increasing the expression level of ovotransferrin. Three different molecular chaperones PrsA, DnaK and CasA are further selected for co-expression with the ovotransferrin, correct folding of the ovotransferrin in host cells is promoted, the soluble secretory expression quantity of the ovotransferrin is increased, and the expression efficiency of the ovotransferrin is improved.