57 results about "Bacillus amyloliquefaciens" patented technology

The application provides preparation of tobacco stalk hydrolysis fermentation and application thereof in crop salt resistance, and relates to the technical field of agriculture.The tobacco stalk hydrolysis fermentation of the application is obtained by microwave-assisted hydrolysis of tobacco stalk to obtain a hydrolysis product, then the hydrolysis product is subjected to fermentation treatment by using bacillus amyloliquefaciens Cas02 to obtain a fermentation crude extract, and after freeze-drying, ethyl acetate extraction is carried out, and the residual solid phase insoluble in ethyl acetate is the tobacco stalk hydrolysis fermentation. The hydrolysis fermentation can improve the salt tolerance of crops, and can effectively alleviate the inhibition of saline-alkali stress on the growth of crops.

This invention provides a biological compound preparation for repelling insects or reducing the oviposition of flying insects, its preparation method, and its application. The biological compound preparation for repelling insects or reducing the oviposition of flying insects includes a chemical reagent and a biological agent; wherein the chemical reagent includes 2-phenylethanol, and the biological agent includes *Bacillus amyloliquefaciens* and *Bacillus brevis*; the preservation number of *Bacillus amyloliquefaciens* is CGMCC No. 35162, and the preservation number of *Bacillus brevis* is CGMCC No. 35161. The core advantage of this invention lies in the synergistic effect of 2-phenylethanol (chemical reagent) with *Bacillus amyloliquefaciens* (CGMCC No. 35162) and *Bacillus brevis* (CGMCC No. 35161) (biological agent). Through complementary and mutually reinforcing mechanisms, the two significantly enhance the control effect.

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The present application relates to the technical field of microbial degradation of rhizoma polygoni multifidi residues, and discloses a composite microbial agent and a preparation method and application thereof.The composite microbial agent contains trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis; wherein the proportion of the spores or spores of the trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis in the composite microbial agent is 1-6:1-6:1-6:1-6:1-6:1-6:1-6.Based on this, the composite microbial agent has good degradation efficiency on rhizoma polygoni multifidi residues, can accelerate the composting process of rhizoma polygoni multifidi residues, shorten the composting period of rhizoma polygoni multifidi residues, and the composting product after composting is stable in quality and is a high-quality organic fertilizer, so that effective utilization of resources is realized.

This invention discloses a Bacillus amyloliquefaciens strain expressing bovine lactoferrin, its construction method, and its applications, belonging to the field of bioengineering technology. This invention utilizes the highly efficient protein secretion capacity of Bacillus amyloliquefaciens NFΔSE and the constitutive expression vector pMA5 for recombinant expression of bovine lactoferrin. Further, extracellular secretory expression of lactoferrin is achieved through signal peptide screening, and efficient fermentation synthesis of lactoferrin is realized in a fermenter through strategies such as optimized combined nutrient conditions and enhanced iron supply. Compared to traditional extraction processes, this process can produce lactoferrin through fermentation using this recombinant strain, effectively improving lactoferrin production capacity and demonstrating good industrialization potential.

This invention discloses a plant-derived preservative for Akebia quinata, its preparation method, and its application, belonging to the field of fruit and vegetable preservation technology. The preservative uses young Akebia quinata fruit as the main raw material, combined with Sophora flavescens, Phellodendron chinense, and Zanthoxylum bungeanum, and is prepared through primary yeast fermentation and secondary fermentation with Lactobacillus plantarum and Bacillus amyloliquefaciens. The preservative significantly reduces polyphenol oxidase (PPO) activity, effectively inhibiting enzymatic browning in Akebia quinata; after 14 days of storage at room temperature, the rot rate decreases to 64%-66%, with effects comparable to imazalil; it delays fruit cracking, reduces the cracking rate, and decreases the length and width of the ventral suture; it also has a significant inhibitory effect on Escherichia coli and Staphylococcus aureus. The product of this invention is purely plant-derived, with no chemical residues, and possesses four functions: inhibiting browning, preventing rot, preventing fruit cracking, and broad-spectrum antibacterial activity. The process is stable, and the application is simple, making it suitable for post-harvest green preservation of Akebia quinata and showing good industrialization prospects.

The application discloses a bacillus zoogleoides BZ-32 and application of metabolites of the bacillus zoogleoides BZ-32 in vegetable stress resistance, and an effective component of the application is composed of bacillus zoogleoides fermentation liquor or metabolites, choline chloride and lentinan, wherein the weight percentage of the bacillus zoogleoides, choline chloride and lentinan is respectively 5-30% of bacillus zoogleoides metabolites, 5-30% of choline chloride and 0.2-5.0% of lentinan. The plant stress resistance composition can prevent and treat diseases such as root rot, anthracnose and powdery mildew of vegetables, and can prevent stress caused by excessively low temperature in winter and spring frost, and improve disease resistance and stress resistance of crops. The application can also be used for preventing and treating various corn diseases and strawberry diseases, and promoting growth and improving stress resistance.

This invention belongs to the field of biotechnology and discloses a strain of Bacillus amyloliquefaciens ZJ02 that inhibits bacterial growth and reduces ammonia nitrogen. Its accession number is CCTCC NO: M20252399, the accession date is October 30, 2025, and the depositary institution is the China Center for Type Culture Collection, located at Wuhan University, Wuhan, China. ZJ02 exhibits significant antibacterial effects against Escherichia coli and Salmonella, with an inhibition zone size of 18.11 mm against Salmonella (SA) after 24 hours. Compared to other strains, it demonstrates superior ammonia nitrogen degradation under the same conditions, achieving a degradation rate of 76.58%, reaching as high as 87.57% at an initial ammonia nitrogen concentration of 500 mg / L. Furthermore, it possesses excellent ability to reduce ammonia release from animal feces. In ammonia removal tests conducted in pig farm environments, compared to the control group, the NH3 concentration decreased by 60.27% on day 6, significantly outperforming Bacillus subtilis and Bacillus licheniformis. This invention also provides the application of this Bacillus amyloliquefaciens.

This invention relates to the field of agricultural plant protection technology, specifically to an integrated pest management method for rapeseed suitable for aerial application, comprising the following steps: mixing Bacillus amyloliquefaciens Bam22 with imidacloprid and cyazofamid for aerial application; wherein, the Bacillus amyloliquefaciens Bam22 bacterial solution is diluted to a concentration of (2.5-5.0) × 10⁻⁶. 7 The dosage of cfu / mL was reduced to (6-9) g / mu for imidacloprid and (18-26) g / mu for boscalid. This invention achieves a synergistic effect by combining the pathogen-inhibiting effect of the Bam22 strain with the targeted control function of chemical agents. This significantly reduces the concentration of chemical agents and avoids pesticide damage from aerial spraying while ensuring that the efficacy of pest and disease control is not reduced. At the same time, the growth-promoting function of the Bam22 strain is used to restore the growth status of rapeseed, enhance the plant's stress resistance, and further ensure the yield and quality of rapeseed.

This invention provides a method for constructing a high-yield ergothionein-producing Bacillus amyloliquefaciens engineered strain and its application. The engineered strain uses the food-grade safe strain Bacillus amyloliquefaciens HZ12 as the starting strain. Through genetic engineering, a key enzyme gene for ergothionein biosynthesis is heterologously expressed in Bacillus amyloliquefaciens. An RBS co-expression strategy is employed to express the mutant gene NcEgt1 derived from Neurospora crassa. Y174F / E51A Co-expression of the EgtE gene derived from *Mycobacterium smegmatis* successfully achieved heterologous synthesis of ergothioneine in *Bacillus amyloliquefaciens*. Under these conditions, the yield of ergothioneine reached 402.68 mg / L, marking the achievement of ergothioneine production in *Bacillus amyloliquefaciens* from scratch. Subsequently, through chassis strain modification, the yield of ergothioneine was further increased to 690.43 mg / L.

This invention discloses a cultivation method for increasing the starch content of duckweed, relating to the field of plant culture technology, comprising the following steps: taking pig farm biogas slurry, adjusting ammonia nitrogen to 10 mg / L~20 mg / L, total phosphorus to 0.1 mg / L~2.0 mg / L, and pH to 5.0~6.0, then inoculating initial duckweed into it and culturing for 5~7 days to obtain acclimatized duckweed; taking pig farm biogas slurry, adjusting ammonia nitrogen to 40~50 mg / L, total phosphorus to 0.4 mg / L~5.0 mg / L, and pH to 5.0~6.0, then adding sodium pyrophosphate and Bacillus amyloliquefaciens, then inoculating the acclimatized duckweed into it and culturing for 2~3 days to obtain high-starch duckweed. This invention constructs a two-step cultivation system of acclimatization and stress through culture solutions with differentiated nitrogen and phosphorus concentrations, and with the synergistic effect of sodium pyrophosphate and Bacillus amyloliquefaciens, achieves efficient and stable accumulation of duckweed starch.

The application discloses an anti-reversibility microbial agent and application thereof in multi-element composite pollution prevention and control, and belongs to the technical field of environmental microbiology and soil remediation. The microbial agent is prepared from a modified bran carrier and a composite bacterial liquid of Bacillus dabanchenensis and Bacillus amyloliquefaciens. After application in saline-alkali and heavy metal composite pollution soil, the microbial agent can significantly improve rhizosphere colonization capacity of the functional strains, greatly reduce soil available Cd and Pb contents, improve soil tillage, inhibit heavy metal eluviation and migration, reduce Cd and Pb contents in corn kernels, and significantly improve crop yield, so that the synergistic effect of the two strains, environmental remediation and agricultural safety are realized.

This invention relates to the field of agricultural microbial fertilizer technology, specifically to a method for preparing and applying a microbial fertilizer for corn cultivation. The microbial fertilizer uses *Bacillus amyloliquefaciens* (BAM). Bacillus amyloliquefaciens B2 is the core functional microorganism, and its fermentation broth is inoculated into a fermentation system containing animal manure, straw powder, modified biochar, and urea for composting fermentation. The modified biochar is obtained by modifying corn straw biochar with magnesium chloride hexahydrate, attapulgite, dicyandiamide, Gly-Pro, and a combination of ferulic acid and tannic acid. This microbial fertilizer can improve the activity of microorganisms and nutrient conversion efficiency during corn cultivation, promoting plant growth. It can also improve the activity of microorganisms and nutrient conversion efficiency in the soil, improve the rhizosphere microecological environment, promote the absorption and utilization of nutrients by corn, thereby promoting plant growth, increasing biomass accumulation, reducing the amount of chemical fertilizer used, and improving the soil ecological environment.

This invention belongs to the field of agricultural microbiology technology, specifically relating to a strain of *Bacillus amyloliquefaciens* and its application in controlling lenticular spot disease in olives. The *Bacillus amyloliquefaciens* disclosed in this invention (…) Bacillus amyloliquefaciens This strain was deposited on April 22, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 37475. The 16S rDNA sequence of this strain is shown in SEQ ID NO:1, and it possesses nitrogen-fixing and phosphorus-solubilizing abilities, exhibiting activity against the pathogen of olive peacock spot disease (…). Cycloconium oleaginum It exhibits significant antagonistic effects, with an inhibition rate of 94.8% in a three-point confrontation test and a field prevention effect of 64.63%. This invention also provides a microbial agent containing this strain and a method for preparing its fermentation broth. Foliar spraying can effectively reduce the disease index of olive peacock spot disease, exhibiting environmental friendliness and significant control efficacy, making it suitable for green control systems for olives.

The application provides a composite microbial liquid for saline soil improvement and crop growth promotion and application thereof, and the composite microbial liquid comprises Bacillus amyloliquefaciens S1-13 liquid, Bacillus thuringiensis SP1-14 liquid, Pseudomonas putida SE1-6 liquid and Bacillus pacificus RC3-7 liquid. Each strain in the composite microbial liquid has specific functions, and after reasonable compounding, the composite microbial liquid can significantly reduce the pH of the soil, improve the soil microbial activity and increase the crop yield.

The application discloses a feed for feeding bullfrog, which comprises photosynthetic bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protein hydrolytic enzyme; the sugar component comprises fructose and glucose; the bone source component is bovine bone powder; and the auxiliary nutritional component is at least one selected from vitamin premix, mineral premix and amino acid premix. The formula is scientific and reasonable, and the feed is comprehensive and balanced in nutrition. The biological bacteria, sugar, bone source and various auxiliary nutritional components synergistically act to meet the requirements of bullfrog at various growth stages. The added biological bacteria and enzymes can improve the digestion and utilization rate, reduce the nutrition waste and breeding cost. The antioxidant and the mildew-proof agent guarantee the good storage performance of the feed, prolong the shelf life and ensure the edible safety. The raw materials and production are strictly controlled, the biological safety is enhanced, and the disease risk is reduced.

The present application belongs to the technical field of microorganisms, and particularly relates to a microbial agent and application thereof in prevention and treatment of persimmon anthracnose. The microbial agent comprises Bacillus amyloliquefaciens QGFCH3-3 and Bacillus atrophaeus HHQGTS13-1. The microbial agent can effectively inhibit the persimmon anthracnose pathogen and has a good prevention and treatment effect on the persimmon anthracnose.

This invention relates to the field of medicinal plant cultivation technology, specifically a cultivation method and application for significantly increasing the mannan content of Dendrobium officinale. The method includes: selecting a suitable cedar forest environment, fixing Dendrobium officinale seedlings using a soilless planting method, watering regularly, and spraying with a 200-fold diluted indigenous probiotic cultivation enzyme every 10 days, for a total of 10 sprays. The enzyme is fermented from native microorganisms in natural forest soil and plant materials, containing Bacillus amyloliquefaciens, yeast, active enzymes, and various minerals. This invention can significantly increase the yield of Dendrobium officinale and the content of active ingredients such as mannan and total amino acids, achieving green, efficient, and sustainable cultivation.

The application discloses a kind of production lipopeptide surfactant and utilize the remediation method of the surfactant to oil hydrocarbon contaminated soil.The preparation method of the surfactant includes: to Bacillus amyloliquefaciens A3 strain is carried out fermentation culture, obtain the fermentation broth containing surfactant;Bacillus amyloliquefaciens A3 strain, preservation number is CGMCC No.6676.The optimal conditions for producing surfactant are: carbon source glycerol, nitrogen source urea, temperature is 25 DEG C, pH is 8.0, carbon source content is 40g / L.The maximum yield of surfactant can be obtained at 150rpm, 72h cultivation is 1.4114g / L.The product of the application has good emulsifying activity and surface activity, and the stability under harsh conditions is also very high, and has very good leaching effect in simulated in-situ remediation soil column leaching experiment compared with control group, and promotes the biodegradation of in-situ soil, and can be used for the remediation of oil hydrocarbon contaminated soil.

The present application belongs to the technical field of biological medicine, and particularly relates to a composition for improving cognitive function, a preparation method, products and applications thereof. The composition is prepared from soybean meal powder, whey protein peptide powder and bovine collagen peptide powder in a mass ratio of 1:3:2 as a peptide source raw material, in combination with AMY001, Lactobacillus plantarum LP-90 and Clostridium butyricum (3:1:1) composite strains, through Bacillus amyloliquefaciens enzyme production self-digestion, peptide-bacteria anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. The composition can work synergistically through multiple mechanisms such as clearing hippocampal Aβ1-42 protein, increasing BDNF content and inhibiting IL-6 expression, has high memory improvement rate and Aβ1-42 clearance rate, and can significantly repair cognitive impairment. The composition has high stability and bioavailability, the preparation process is controllable, and is suitable for the development of drugs for preventing or assisting in improving cognitive impairment related diseases.

The present application relates to the technical field of food fermentation, in particular to a method for improving the taste of corn noodles by multi-microbial synergistic fermentation of corn and application thereof. The method provided by the present application uses lactic acid bacteria, Bacillus amyloliquefaciens and yeast to synergistically ferment corn to prepare fermented corn flour. Multi-microbial fermentation can effectively improve the processing characteristics of corn flour and enhance the antioxidant, free radical removal and other health and probiotic functions of corn flour. Further, the fermented corn flour is mixed with puffed corn flour and corn starch at a specific ratio to prepare pure corn noodles, which have significant advantages in viscoelasticity and ductility and better comprehensive texture characteristics and sensory scores.

This invention provides a composite protectant for spray drying of *Bacillus amyloliquefaciens*, composed of the following raw materials in the indicated mass fractions: 60.0% soy protein isolate, 30.0% lactose, and the balance being gum arabic. A method for preparing *Bacillus amyloliquefaciens* powder using this composite protectant is also provided. The composite protectant is mixed with light calcium carbonate to obtain a solid, which is then added to a *Bacillus amyloliquefaciens* bacterial solution. After stirring and mixing, the mixture is spray-dried to obtain *Bacillus amyloliquefaciens* powder. The prepared *Bacillus amyloliquefaciens* powder is used for the biological control of tomato nematode disease. The composite protectant of this invention can significantly increase the viable count of *Bacillus amyloliquefaciens* after spray drying, reduce production costs, minimize resource waste, and provide technical support for the industrial-scale preparation of microbial agents.

This invention discloses a Bacillus microbial agent for controlling tobacco diseases, its preparation method, and its application. The microbial agent is prepared by compounding Bacillus amyloliquefaciens and Bacillus bellis in a volume ratio of 0.8-1.2:1, resulting in a total effective viable count ≥2×10¹⁰ CFU / mL. This invention utilizes independently isolated specific Bacillus amyloliquefaciens and Bacillus bellis; the compounding of these two agents creates a synergistic effect. Bacillus amyloliquefaciens secretes antibacterial substances that directly inhibit the growth of pathogens, while Bacillus bellis induces resistance in tobacco plants and enhances rhizosphere colonization, synergistically improving the control effect against tobacco bacterial wilt and black shank. Field trials show that this microbial agent achieves a control efficacy of over 75% against tobacco bacterial wilt and over 85% against black shank, significantly higher than single-strain agents. The strains used in this invention are isolated from the rhizosphere soil of tobacco plants in different tobacco-growing areas of Baoshan. The addition of a special stabilizer after compounding enhances the strains' resistance, extends shelf life at room temperature, and maintains stable efficacy.

The present application relates to a kind of suitable for poplar growth regulating composition and biological preparation, including functional bacteria agent and chemical trigger complex;Functional bacteria agent includes Bacillus amyloliquefaciens and poplar canker antagonistic Trichoderma asperellum;Chemical trigger complex includes the directional metabolic promotion group for promoting the directional synthesis of functional bacteria agent plant hormone, the metal cofactor signal group for strengthening the effect of directional metabolism, and the fermentation microenvironment regulation group for providing initial microenvironment support for the fermentation of functional bacteria agent under poplar;Directional metabolic promotion group includes L-tryptophan and adenine;Metal cofactor signal group includes manganese ion signal and magnesium ion signal;Fermentation microenvironment regulation group includes humic acid salt, mineral phosphorus source, clay mineral carrier and sugar protective agent.The present application can solve the problems existing in the prior art, such as the difficulty of regulating composition to realize the directional growth of poplar trunk, the need for multiple applications, the high risk of ecological residue, and the difficulty of cost to adapt to the large-area cultivation of poplar.

The present application relates to the technical field of pet skin care, and specifically discloses a preparation for preventing or treating pet skin fungal infection and a preparation method thereof, which adopts an aerobic-anaerobic ladder type composite fermentation process, takes coix seed, mugwort leaf and purslane as substrates, inoculates bacillus amyloliquefaciens for aerobic fermentation, utilizes enzyme production of the bacillus amyloliquefaciens to break the wall of the herbal components and enriches lipopeptide substances, then inoculates lactobacillus rhamnosus and propionibacterium freudenreichii for anaerobic fermentation, releases free glycoside bases through mutual metabolism, synthesizes short-chain fatty acids such as propionic acid, and obtains the preparation after centrifugal sterilization. The present application realizes the organic integration of the herbal efficacy and the microecological metabolism, can effectively inhibit pathogenic fungi such as microsporum canis, has multiple effects of anti-inflammatory, antipruritic and repairing pet skin barrier, is safe and mild, and has a stable and controllable process.

This invention discloses a method for promoting rice growth using a compound microbial agent and humic acid. Humic acid and the compound microbial agent are added sequentially to rice-grown soil. The amount of humic acid added is 0.5-2% of the soil mass, preferably 1% of the soil mass. The solid-liquid ratio of humic acid to the compound microbial agent (g:ml) is 0.5-1.1:15-30, preferably 1:20. The compound microbial agent is obtained by mixing any two or more monofunctional bacteria in equal volume ratios. The monofunctional bacteria include *Bacillus amyloliquefaciens* FH1, *Bacillus shortwave diversicolor* NH1, *Glucosidobacterium tumefaciens* QZR14, *Bacillus cereus* S112, and *Pantothecinus flocculationensis*. 、 The compound microbial agent of this invention, along with humic acid, can effectively promote rice growth, optimize soil microbial community and function, and provide a scientific basis for the research and application of new compound microbial fertilizers, thus contributing to the development of sustainable agriculture.