12 results about "Plasmodiophora brassicae" patented technology

The invention relates to the technical field of plant disease control, and particularly discloses a screening method of a cruciferous crop clubroot control agent, which comprises the following steps: after accelerating germination of cruciferous crop seeds, transplanting the cruciferous crop seeds into a 1 / 2 MS solution, and culturing the cruciferous crop seeds to a two-leaf one-core stage to obtain seedlings; soaking the roots of the seedlings in a mixed system containing plasmodiophora brassicae spores and a medicament to be detected, and inoculating; and transplanting the inoculated plants into a newly prepared 1 / 2 MS solution for continuous culture, and regularly investigating morbidity and prevention and control effects to screen out the medicament with prevention and control effects on the clubroot of the cruciferae crops. According to the method provided by the invention, continuous and non-destructive dynamic observation on the same plant can be allowed, the disease progress is accurately monitored, and the medicament screening cost is greatly reduced.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a calmodulin-binding protein CBP60g or a coding gene CBP60g thereof in control of clubroot, wherein an amino acid sequence of the calmodulin-binding protein CBP60g is shown as SEQ ID NO. 1. The application can restore the resistance of Arabidopsis thaliana to Plasmodiophora brassicae at high temperature by overexpressing CBP60g, and the same is verified in Brassica napus, and the normal growth, yield and quality of the Brassica napus are not affected, which indicates that the CBP60g gene can resist Plasmodiophora brassicae at high temperature, and increase the recovery of the immune response of crops to high-temperature combined stress, and provides a strategy for molecular breeding in response to climate change.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The present application relates to the technical field of microorganisms, and particularly relates to a bacillus velezensis F0b and application thereof.The bacillus velezensis F0b has been preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, and the preservation number is GDMCC No: 64566.The bacillus velezensis has a preventive and / or control effect on rice blast fungus, plasmodiophora brassicae, fusarium solani, fusarium oxysporum f.sp.cubense, rice leaf spot fungus, pythium sylvaticum, diaporthe phaseolorum, colletotrichum gloeosporioides and curvularia inaequalis, and can be used to prepare a broad-spectrum microbial source bactericide.The bacillus velezensis can produce amylase, carboxymethyl cellulase and protease in fermentation liquor, and the fermentation liquor of the bacillus velezensis has a preventive and / or control effect on vegetable pathogenic fungal diseases, such as cabbage sclerotinia disease or pepper anthracnose disease.

The invention belongs to the field of biological agriculture, and relates to application of helicase PbDDX15-like in prevention and control of clubroot. According to the invention, a core gene of plasmodiophora brassicae ZJ-1 is compared with a brewer's yeast protein sequence, a gene DEAD-box RNA helicase (DEAD-box RNA helicase 15-like, PbDDX15-like) which may have a lethal effect on plasmodiophora brassicae is screened out, an RNAi vector of the gene is constructed by using an HIGS technology, and the RNAi vector is transformed into wild type arabidopsis thaliana. The PbDDX15-like-RNAi transgenic arabidopsis thaliana is subjected to a plasmodiophora brassicae disease resistance experiment, and the result shows that the PbDDX15-like-RNAi transgenic arabidopsis thaliana has remarkable resistance to plasmodiophora brassicae, so that the protein can provide a new gene resource for green prevention and resistance of the rape clubroot.

The invention discloses an application of a targeted ABC transporter PbABCB25 in prevention and control of clubroot of rape. ABC transporter protein ABCB25 (abc transporter b family meber 25, ABCB25) with high-level expression in each stage is screened out from a plasmodiophora brassicae ZJ-1 transcriptome, then an RNAi carrier of the ABC transporter protein ABCB25 is constructed through an HIGS technology, the RNAi carrier is transformed into wild type arabidopsis thaliana, then the resistance of transgenic arabidopsis thaliana to clubroot is tested, the result shows that PbABCB25 has remarkable resistance to plasmodiophora brassicae, and the PbABCB25 can be used for preparing the plasmodiophora brassicae. The result shows that the ABCB25 in the plasmodiophora brassicae can be used as a gene resource for resisting the clubroot of the cruciferae.

The invention discloses bacillus deserticola B-3 with broad-spectrum antibacterial activity and an application of the bacillus deserticola B-3 with the broad-spectrum antibacterial activity. Bacillus deserticola B-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the preservation date is August 28, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.35756. The B3 strain is bacillus deserticola with plasmodiophora brassicae antagonistic capacity reported for the first time, has an antagonistic effect on various phytopathogens including botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii, can be used as a biopesticide, has a good effect on clubroot, and can be applied to the field of biological pesticides. After the B-3 is used, the fresh weight of a plant infected by the plasmodiophora brassicae Pb4 is remarkably increased, and the number of plant root systems is remarkably increased, so that the biocontrol bacterium can effectively prevent and treat clubroot and has the effects of inhibiting bacteria and promoting growth.

The invention belongs to the field of biopesticides, and relates to application of a metabotropic glutamate receptor PbMGR in prevention and control of rape clubroot. The application of the metabotropic glutamate receptor PbMGR gene in improving the clubroot resistance of plants and / or cultivating clubroot-resistant plants is characterized in that the CDS sequence of the gene is as shown in SEQ ID NO.1. The invention also discloses an application of the metabotropic glutamate receptor PbMGR gene in improving the clubroot resistance of plants and / or cultivating clubroot-resistant plants. According to the invention, G protein coupled receptors (metabotropic glutamate receptors, PbMGR) which are expressed at a high level in each stage are screened from a plasmodiophora brassicae ZJ-1 transcriptome, an RNAi carrier of the gene is constructed, corresponding transgenic plants are obtained, and disease resistance tests of plasmodiophora brassicae show that two PbMGR-RNAi transgenic strains both show good disease resistance phenotypes, and the two PbMGR-RNAi transgenic strains show good disease resistance phenotypes. Therefore, the G protein coupled receptor PbMGR of plasmodiophora brassicae can be used as a gene resource for resisting clubroot.

The invention relates to the technical field related to gene engineering, in particular to application of brassica juncea BjuB01. JAL34 in clubroot resistance of brassica napus. The CDS sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.1, and the coded amino acid sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.2. The invention also relates to application of the gene BjuB01. JAL34 in clubroot resistance of brassica napus. According to the invention, the gene is over-expressed in a model plant arabidopsis thaliana and an important commercial crop brassica napus through a genetic transformation technology, so that the resistance of the gene to plasmodiophora brassicae No.4 physiological race can be remarkably improved; the BjuB01. JAL34 gene and the protein coded by the BjuB01. JAL34 gene can be used for culturing broad-spectrum and durable clubroot-resistant brassica napus and other new species of cruciferae crops, and have important theoretical value and wide application prospect.

The invention discloses a method for creating a brassica napus resistant plant by using an R5 monomer addition line, and belongs to the field of molecular marker-assisted breeding. The method for creating the brassica napus resistant plant provided by the invention comprises the following steps: backcrossing a brassica napus-turnip cabbage R5 monomer addition line and brassica napus Zhongdou-11, screening backcrossing offspring by using a molecular marker to obtain a plant with broken R5 chromosome, and then carrying out inoculation verification to obtain a resistant single plant. Experimental results show that the additional R5 chromosome segment of the resistant single plant obtained by the method provided by the invention has outstanding resistance to plasmodiophora brassicae in a plurality of regions. According to the invention, the process of introducing the radish R chromosome segment into the brassica napus can be accelerated, the disease-resistant interval can be positioned, new germplasm resources and technical basis are provided for breeding clubroot-resistant varieties, and the breeding time is shortened.