27 results about "Plasmodiophora brassicae" patented technology

The invention relates to the technical field of plant pathogen identification, and provides primers and a method for rapidly detecting and identifying pathotypes of plasmodiophora brassicae based on a PCR (Polymerase Chain Reaction) technology. Comprising an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 3; any one of the upstream primer and the downstream primer forms a primer. Identification of different pathotypes of plasmodiophora brassicae is rapidly completed through the PCR technology, and especially, the pathotype of plasmodiophora brassicae to cruciferae crops is rapidly and accurately identified through the difference of PCR amplification results; the identification result is accurate and repeatable, the operation process is simplified, the detection time is reduced, and the operation threshold is low.

The invention provides an endophytic pseudomonas kirscheri and an application of the endophytic pseudomonas kirscheri. The pseudomonas is endophytic pseudomonas kilonensis Pk-5, and the preservation number of the pseudomonas kilonensis Pk-5 is CGMCC (China General Microbiological Culture Collection Center) No. 32570. The strain can improve the biomass of plants under normal and sick conditions by inhibiting the pathogenic effect of plasmodiophora brassicae; after plants are inoculated with plasmodiophora brassicae, the pseudomonas Pk-5 can obviously promote the growth of the plants.

The invention discloses an anti-clubroot gene BolC6. MYB122 and application thereof, and relates to the technical field of plant biology. The NDA sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 1, and the CDS sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 2. A constructed 35S: BolC6. MYB122-eGFP overexpression vector is transformed into wild type arabidopsis thaliana, an arabidopsis thaliana myb122 mutant is obtained, namely an overexpression transgenic line is obtained, the resistance of overexpression positive regulation of the BolC6. MYB122 gene to plasmodiophora brassicae is clarified through disease resistance identification, and the transgenic line is a transgenic line. And a theoretical basis and gene resources are provided for clubroot-resistant molecular breeding of plants such as brassica napus.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Ataf1. The invention discloses application of inhibiting expression of an arabidopsis S gene Ataf1 in enhancing resistance of plants to pathogenic bacteria. The CDS sequence of the arabidopsis S gene Ataf1 is shown as SEQ ID NO. 1. According to the invention, an S gene Ataf1 of arabidopsis thaliana is selected, the growth phenotype of a homozygous mutant of the gene constructed by a T-DNA insertion method is observed, and inoculation experiments are carried out on the homozygous mutant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Ataf1 mutant is not influenced, the Ataf1 mutant shows obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Ataf1 mutant are obviously lower than those of a wild plant. Therefore, the S gene Ataf1 of arabidopsis thaliana can be used as a potential gene resource for a green prevention and control strategy of sclerotinia rot of colza.

The invention relates to the technical field of plant disease control, and particularly discloses a screening method of a cruciferous crop clubroot control agent, which comprises the following steps: after accelerating germination of cruciferous crop seeds, transplanting the cruciferous crop seeds into a 1 / 2 MS solution, and culturing the cruciferous crop seeds to a two-leaf one-core stage to obtain seedlings; soaking the roots of the seedlings in a mixed system containing plasmodiophora brassicae spores and a medicament to be detected, and inoculating; and transplanting the inoculated plants into a newly prepared 1 / 2 MS solution for continuous culture, and regularly investigating morbidity and prevention and control effects to screen out the medicament with prevention and control effects on the clubroot of the cruciferae crops. According to the method provided by the invention, continuous and non-destructive dynamic observation on the same plant can be allowed, the disease progress is accurately monitored, and the medicament screening cost is greatly reduced.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a calmodulin-binding protein CBP60g or a coding gene CBP60g thereof in control of clubroot, wherein an amino acid sequence of the calmodulin-binding protein CBP60g is shown as SEQ ID NO. 1. The application can restore the resistance of Arabidopsis thaliana to Plasmodiophora brassicae at high temperature by overexpressing CBP60g, and the same is verified in Brassica napus, and the normal growth, yield and quality of the Brassica napus are not affected, which indicates that the CBP60g gene can resist Plasmodiophora brassicae at high temperature, and increase the recovery of the immune response of crops to high-temperature combined stress, and provides a strategy for molecular breeding in response to climate change.

This invention belongs to the fields of agricultural plant pathology and plant protection technology, and relates to a clubroot bacterium Pb034 gene and its application in the control of clubroot disease in cruciferous crops. This invention found that reducing the expression of the clubroot bacterium Pb034 gene using host-induced gene silencing (HIGS) technology or exogenous double-stranded RNA delivery technology is beneficial for enhancing the resistance of cruciferous plants to clubroot disease. This invention provides a new approach and method for the control of clubroot disease in cruciferous crops, and has broad agricultural application prospects and market value.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

This invention relates to the field of Streptomyces technology, and discloses a *Streptomyces nigricans* strain for controlling clubroot disease in cruciferous plants in the field, its screening method, and the application of fermentation broth from derived strains. The *Streptomyces nigricans* strain, named X216, was isolated and screened from soil in cruciferous vegetable fields affected by clubroot disease and deposited at the China Center for Type Culture Collection (CCTCC) on May 10, 2023, with accession number CCTCC No: M2023723. This invention utilizes *Streptomyces nigricans*, an antagonistic strain against indicator fungi, screened from rapeseed fields affected by clubroot disease. Compared to existing chemical pesticides, it effectively increases the lethality of dormant *Plasmodiophora brassicae* spores and reduces the root hair infection rate of dormant *Plasmodiophora brassicae* spores, demonstrating a significant effect on clubroot disease control. Furthermore, its direct application in natural field environments shows significant efficacy in controlling clubroot disease in cruciferous plants.

The present application belongs to the field of agricultural plant pathology and plant protection technology, and relates to a Plasmodiophora brassicae Pb035 gene and application thereof in prevention and control of Plasmodiophora brassicae. The core of the present application is to use host-induced gene silencing (HIGS) technology or exogenous RNA interference fragment delivery technology to silence Plasmodiophora brassicae Pb035 gene expression, thereby enhancing the resistance of Brassicaceae crops to Plasmodiophora brassicae. The present application provides a key technology and method for prevention and control of Plasmodiophora brassicae, and has broad agricultural application prospect and market value.

The present application relates to the technical field of microorganisms, and particularly relates to a bacillus velezensis F0b and application thereof.The bacillus velezensis F0b has been preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, and the preservation number is GDMCC No: 64566.The bacillus velezensis has a preventive and / or control effect on rice blast fungus, plasmodiophora brassicae, fusarium solani, fusarium oxysporum f.sp.cubense, rice leaf spot fungus, pythium sylvaticum, diaporthe phaseolorum, colletotrichum gloeosporioides and curvularia inaequalis, and can be used to prepare a broad-spectrum microbial source bactericide.The bacillus velezensis can produce amylase, carboxymethyl cellulase and protease in fermentation liquor, and the fermentation liquor of the bacillus velezensis has a preventive and / or control effect on vegetable pathogenic fungal diseases, such as cabbage sclerotinia disease or pepper anthracnose disease.

The invention belongs to the field of biological agriculture, and relates to application of helicase PbDDX15-like in prevention and control of clubroot. According to the invention, a core gene of plasmodiophora brassicae ZJ-1 is compared with a brewer's yeast protein sequence, a gene DEAD-box RNA helicase (DEAD-box RNA helicase 15-like, PbDDX15-like) which may have a lethal effect on plasmodiophora brassicae is screened out, an RNAi vector of the gene is constructed by using an HIGS technology, and the RNAi vector is transformed into wild type arabidopsis thaliana. The PbDDX15-like-RNAi transgenic arabidopsis thaliana is subjected to a plasmodiophora brassicae disease resistance experiment, and the result shows that the PbDDX15-like-RNAi transgenic arabidopsis thaliana has remarkable resistance to plasmodiophora brassicae, so that the protein can provide a new gene resource for green prevention and resistance of the rape clubroot.

The invention discloses an application of a targeted ABC transporter PbABCB25 in prevention and control of clubroot of rape. ABC transporter protein ABCB25 (abc transporter b family meber 25, ABCB25) with high-level expression in each stage is screened out from a plasmodiophora brassicae ZJ-1 transcriptome, then an RNAi carrier of the ABC transporter protein ABCB25 is constructed through an HIGS technology, the RNAi carrier is transformed into wild type arabidopsis thaliana, then the resistance of transgenic arabidopsis thaliana to clubroot is tested, the result shows that PbABCB25 has remarkable resistance to plasmodiophora brassicae, and the PbABCB25 can be used for preparing the plasmodiophora brassicae. The result shows that the ABCB25 in the plasmodiophora brassicae can be used as a gene resource for resisting the clubroot of the cruciferae.

The invention provides a method for improving clubroot resistance of cruciferous plants. It is found for the first time that probenazole treatment can improve the resistance of Chinese cabbages to clubroot and inhibit the vitality of plasmodiophora brassicae spores. The morbidity and the disease index of the Chinese cabbage clubroot can be reduced through pretreatment with probenazole of different concentrations and different dosage forms, the disease development is delayed, and adverse effects on the growth of Chinese cabbages cannot be caused. The probenazole suspending agent and the nanocapsule suspending agent prepared by the invention have outstanding prevention and control effects on clubroot of cruciferous plants.

The application belongs to the technical field of genetic engineering, and particularly relates to application of BnCDS1 gene in plant disease prevention and control and / or growth and an sgRNA combination. The application edits the BnCDS1 gene of rapeseed, and the mutant rapeseed is not different from the wild type Westar in aspects of seedling stage plant phenotype, chlorophyll content, light energy conversion efficiency, nitrogen content and flowering time, but the plant height becomes shorter with the increase of the copy editing number, and the silique becomes smaller and shorter. It is indicated that BnCDS1 is involved in the regulation of rapeseed growth, seed setting and other traits. The edited BnCDS1 rapeseed mutant is inoculated with Sclerotinia sclerotiorum, Botrytis cinerea and Plasmodiophora brassicae, and it is found that the edited rapeseed has enhanced resistance to Sclerotinia sclerotiorum, Botrytis cinerea and Plasmodiophora brassicae, and the higher the editing copy number is, the stronger the resistance is. The BnCDS1 gene edited mutant shows a certain broad-spectrum disease resistance, and the BnCDS1 gene can be used as a new gene resource for green prevention and control of rapeseed diseases.

The invention discloses a primer group for plasmodiophora brassicae as well as a detection method and application of the primer group. The invention provides a primer group. The primer group comprises at least one of a Pb06745-R / F primer group, a Pb08600-R / F primer group and a Pb03781-R / F primer group. The invention provides three qPCR primer groups for quantitative detection of plasmodiophora brassicae of rape seeds, can specifically detect 10 resting spores of plasmodiophora brassicae, has the advantages of high sensitivity and high specificity, can detect different physiological race types of plasmodiophora brassicae, and has the advantage of high stability. A safe and reliable detection technology is provided for oilseed rape production, whether oilseed rape seeds are polluted by plasmodiophora brassicae or not and the bacterium carrying amount can be rapidly and accurately judged, rapid spreading of oilseed rape clubroot is relieved, clubroot is prevented and controlled, and meanwhile the method has guiding significance in inspection and quarantine of entry and exit related plants and products.

The invention discloses bacillus deserticola B-3 with broad-spectrum antibacterial activity and an application of the bacillus deserticola B-3 with the broad-spectrum antibacterial activity. Bacillus deserticola B-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the preservation date is August 28, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.35756. The B3 strain is bacillus deserticola with plasmodiophora brassicae antagonistic capacity reported for the first time, has an antagonistic effect on various phytopathogens including botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii, can be used as a biopesticide, has a good effect on clubroot, and can be applied to the field of biological pesticides. After the B-3 is used, the fresh weight of a plant infected by the plasmodiophora brassicae Pb4 is remarkably increased, and the number of plant root systems is remarkably increased, so that the biocontrol bacterium can effectively prevent and treat clubroot and has the effects of inhibiting bacteria and promoting growth.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

The invention belongs to the field of biopesticides, and relates to application of a metabotropic glutamate receptor PbMGR in prevention and control of rape clubroot. The application of the metabotropic glutamate receptor PbMGR gene in improving the clubroot resistance of plants and / or cultivating clubroot-resistant plants is characterized in that the CDS sequence of the gene is as shown in SEQ ID NO.1. The invention also discloses an application of the metabotropic glutamate receptor PbMGR gene in improving the clubroot resistance of plants and / or cultivating clubroot-resistant plants. According to the invention, G protein coupled receptors (metabotropic glutamate receptors, PbMGR) which are expressed at a high level in each stage are screened from a plasmodiophora brassicae ZJ-1 transcriptome, an RNAi carrier of the gene is constructed, corresponding transgenic plants are obtained, and disease resistance tests of plasmodiophora brassicae show that two PbMGR-RNAi transgenic strains both show good disease resistance phenotypes, and the two PbMGR-RNAi transgenic strains show good disease resistance phenotypes. Therefore, the G protein coupled receptor PbMGR of plasmodiophora brassicae can be used as a gene resource for resisting clubroot.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Atbisr6. The knockout arabidopsis Atbisr6 gene is applied to improvement of the resistance of plants to pathogenic bacteria. A homozygous mutant plant of the gene is obtained by screening and identifying a mutant of the gene constructed by a T-DNA insertion method, the growth phenotype of the mutant plant is observed, and inoculation experiments are performed on the mutant plant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Atbisr6 mutant is not influenced, the Atbisr6 mutant has obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Atbisr6 mutant are obviously lower than those of a wild plant. Therefore, the S gene Atbis6 of arabidopsis thaliana can be used as a potential gene resource, and is applied to a green prevention and control strategy of sclerotinia rot of colza by knocking out the modified gene or inhibiting the expression of the modified gene.

The invention relates to the technical field related to gene engineering, in particular to application of brassica juncea BjuB01. JAL34 in clubroot resistance of brassica napus. The CDS sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.1, and the coded amino acid sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.2. The invention also relates to application of the gene BjuB01. JAL34 in clubroot resistance of brassica napus. According to the invention, the gene is over-expressed in a model plant arabidopsis thaliana and an important commercial crop brassica napus through a genetic transformation technology, so that the resistance of the gene to plasmodiophora brassicae No.4 physiological race can be remarkably improved; the BjuB01. JAL34 gene and the protein coded by the BjuB01. JAL34 gene can be used for culturing broad-spectrum and durable clubroot-resistant brassica napus and other new species of cruciferae crops, and have important theoretical value and wide application prospect.

The invention discloses a compound microorganism bacterium agent for preventing and treating clubroot of cruciferae crops. The compound microorganism bacterium agent comprises a streptomyces roseosporus bacterium agent with the serial number of B98035, a trichoderma citrinoviride bacterium agent with the serial number of BMZ102895, and a bacillus sororensis bacterium agent with the serial number of BMZ065932, and the compound microorganism bacterium agent is prepared from a compound microorganism bacterium agent with the serial number of BMZ065932, a compound microorganism bacterium agent with the serial number of BMZ065932, a compound microorganism bacterium agent with the serial number of BMZ065932 and a compound microorganism bacterium agent with the serial number of BMZ065932 and a compound microorganism bacterium agent with the serial number of BMZ065932. The volume ratio of the streptomyces roseosporus microbial inoculum to the trichoderma citrinoviride microbial inoculum to the bacillus sorola is (1: 2)-(4: 2), the number of effective viable bacteria in the streptomyces roseosporus microbial inoculum is not less than 1 * 10 < 8 > CFU / ml, the number of effective viable bacteria in the trichoderma citrinoviride microbial inoculum is not less than 1 * 10 < 8 > CFU / ml, and the number of effective viable bacteria in the bacillus sorola microbial inoculum is not less than 1 * 10 < 9 > CFU / ml. The complex microbial inoculant disclosed by the invention can be used for effectively preventing and treating the clubroot of the cruciferae crops, which is caused by plasmodiophora brassicae, and can be used for preventing and treating the clubroot of the cruciferae crops, which is caused by the plasmodiophora brassicae.

The invention discloses a colza sclerotiniose clubroot resistance evaluation method, and relates to the technical field of stress-resistant colza screening and evaluation. Comprising the following steps: 8-10 days after rape seedling emergence, diluting the plasmodiophora brassicae spore concentration to 2 * 10 < 6 > / mL to 3 * 10 < 6 > / mL by using sterile water, then mixing a plasmodiophora brassicae spore suspension and a sclerotinia sclerotiorum hypha suspension in equal proportion, and enabling the bacterial suspension liquid to slowly flow down along plant stems and be immersed into root soil along the positions 0.5-1 cm away from the basal parts of rape plant stems; the method comprises the following steps of: culturing at the constant temperature of 25 DEG C and the relative humidity of more than 90% in the presence of light for 16 hours and darkness for 8 hours alternately, observing the symptoms of roots, basal stems or petioles after 30 days, carrying out disease grading of the clubroot and the sclerotiniose, then calculating disease indexes according to the grading and the number of diseased plants at each stage, and further evaluating the resistance grades of the sclerotiniose and the clubroot according to the disease indexes. According to the evaluation method disclosed by the invention, disease occurrence conditions can be created under indoor conditions, so that sclerotinia sclerotiorum and plasmodiophora brassicae infect hosts at the same time, the evaluation time can be shortened, and meanwhile, the defect that the resistance evaluation of different diseases in different growth periods of the rape cannot truly reflect the resistance level of the hosts is overcome.

The invention discloses an application of targeted metal ABC transporter permease PbMABC in prevention and control of clubroot of rape. According to the invention, metal ABC transporter permease MABC (metal abc transporter permelase) with high-level expression at each stage is screened out from a plasmodiophora brassicae ZJ-1 transcriptome, then an RNAi carrier of the metal ABC transporter permease MABC is constructed through an HIGS technology, the RNAi carrier is transformed into wild-type arabidopsis thaliana, then the resistance of transgenic arabidopsis thaliana to clubroot is tested, the result shows that PbMABC has remarkable resistance to plasmodiophora brassicae, and the PbMABC can be used for preparing the plasmodiophora brassicae. The MABC in the plasmodiophora brassicae can be used as a gene resource for resisting the clubroot of the cruciferae.

The invention discloses a method for creating a brassica napus resistant plant by using an R5 monomer addition line, and belongs to the field of molecular marker-assisted breeding. The method for creating the brassica napus resistant plant provided by the invention comprises the following steps: backcrossing a brassica napus-turnip cabbage R5 monomer addition line and brassica napus Zhongdou-11, screening backcrossing offspring by using a molecular marker to obtain a plant with broken R5 chromosome, and then carrying out inoculation verification to obtain a resistant single plant. Experimental results show that the additional R5 chromosome segment of the resistant single plant obtained by the method provided by the invention has outstanding resistance to plasmodiophora brassicae in a plurality of regions. According to the invention, the process of introducing the radish R chromosome segment into the brassica napus can be accelerated, the disease-resistant interval can be positioned, new germplasm resources and technical basis are provided for breeding clubroot-resistant varieties, and the breeding time is shortened.