2results about How to "Environmentally safe" patented technology

The present application relates to the technical field of microbiology and animal vaccine cross, and particularly relates to a gene deletion attenuated mutant of wild strain of Pseudomonas plecoglossicida, a construction method and application thereof, and provides the gene deletion attenuated mutant based on deletion of the coding genes of proteins participating in maintaining cell membrane lipid asymmetry of Pseudomonas plecoglossicida strain on the wild strain of Pseudomonas plecoglossicida mlaE , the coding gene of response regulator protein of envZ / ompR two-component system ompR , and the coding gene of DeoR family transcriptional regulator deoR The mutant strain has obvious low toxicity, can effectively protect the test fish from the invasion of Pseudomonas plecoglossicida causing the disease of Pseudomonas plecoglossicida visceral white spot disease, and researches show that the vaccine prepared based on the gene deletion attenuated mutant has significant immunization effect and excellent prevention and treatment effect on the disease of Pseudomonas plecoglossicida visceral white spot disease in aquaculture.

This invention discloses a method for remediating halogenated hydrocarbon pollution based on functional plasmid transformation, belonging to the field of environmental microbial remediation technology. The method first extracts plasmid DNA carrying functional genes from organobacteria with reductive dechlorination capabilities (organobacteria with halogen respiration). Then, the functional plasmid DNA and electron donors are directly added to halogenated hydrocarbon-contaminated samples containing indigenous microorganisms to construct a bio-enhanced remediation system. Finally, the system is cultured under anaerobic conditions to transform the functional plasmid into the indigenous microorganisms, thereby endowing or enhancing the indigenous microorganisms with the ability to reductively dechlorinate halogenated hydrocarbon pollutants. This invention avoids the ecological risks of directly introducing exogenous live bacteria. Through plasmid-mediated horizontal gene transfer, it rapidly activates and enhances the function of in-situ microbial communities, significantly improving the degradation rate and thoroughness of halogenated hydrocarbon pollutants, and providing an efficient, safe, and universally applicable technical means for in-situ bioremediation of contaminated sites.