10 results about "Response regulator" patented technology

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

The invention discloses an application of a soybean B-type response regulatory factor gene GmRR1 in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean B-type response regulatory factor gene GmRR1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean B-type response regulatory factor gene GmRR1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The present invention discloses a Bacillus subtilis autoinducing gene expression system based on a two-component quorum sensing element, belonging to the field of genetic engineering technology. A long-lasting and high-expression autoinducing gene expression system is developed. The present invention provides an autoinducing gene expression system, wherein the expression element comprises a constitutive promoter and a target promoter, the RBS sequence of the agrA gene and / or the RBS sequence of the agrD gene, the gene agrA encoding a response regulator protein, the gene agrD encoding the precursor of the signal molecule AIP, the gene agrB encoding a signal processing protein, and the gene agrC encoding a sensor protein that binds to the signal molecule and initiates signal transduction. The present invention provides a new approach and method for designing a recombinant protein expression system based on Bacillus subtilis.

The application discloses an Escherichia coli genetically engineered strain for producing pyruvic acid derivative compounds, and discloses a method for improving the yield of pyruvic acid derivative compounds in the Escherichia coli, and discloses the application of the Escherichia coli genetically engineered strain in biosynthesis of poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene. The application can widely enhance the synthetic metabolic flow with pyruvic acid as a key node by knocking out a leucine response regulator gene, and the modification can effectively strengthen the synthesis capacity of the engineered strain for the four target products, i.e., poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene under the condition of various carbon sources. Moreover, the knockout of the gene can specifically promote the lactic acid precursor flux, thereby improving the molar proportion of lactic acid monomers in the product P(3HB-LA). lrp co ​​

The application belongs to the technical field of plant genetic engineering, and particularly relates to a rice cytokinin response regulator gene OsRR11 and application. Research finds that OsRR11 encodes a conserved protein containing an aspartic acid signal receiving domain, and is a specific response regulator of cZ in rice; through research, it is found that OsRR11 has a positive regulation function in the resistance of rice to brown planthopper. Through agrobacterium-mediated genetic transformation, OsRR11 is overexpressed in japonica OsRR11 and is knocked out in Bph6-NIL, the resistance of the overexpression plant to brown planthopper is significantly enhanced, and the resistance of the knockout mutant plant to brown planthopper is significantly weakened. The gene of the application provides a good theoretical basis for studying how cytokinin participates in the insect resistance of rice through a response regulator, has a reference significance for studying the molecular function of a gene, and provides a gene resource for molecular design breeding of rice insect resistance.

The application discloses a rhamnolipid-producing genetically engineered bacterium and application thereof, and belongs to the technical field of microorganisms and genetic engineering. A Psl polysaccharide coding gene cluster and a Pel polysaccharide coding gene cluster of Pseudomonas aeruginosa AB93066 (a starting strain) are knocked out, a coding gene FleQ of a cyclic diguanylate receptor is further knocked out, and a response regulator BfmS is overexpressed to obtain the rhamnolipid-producing genetically engineered bacterium. The strain has the advantages of a short fermentation cycle, strong rhamnolipid synthesis capacity and weak biofilm formation capacity, can effectively improve the production efficiency of rhamnolipid, reduces the biological safety risk, and is suitable for industrial production and application.

The invention provides an application of a cytokinin response regulatory factor ZmARR2 gene and an encoding protein thereof in regulating plant salt tolerance, and belongs to the field of plant genetic engineering breeding. The invention discloses the key effect of ZmARR2 in regulating and controlling the salt tolerance of corn for the first time, and compared with a wild type, a ZmARR2 overexpression strain shows stronger salt stress tolerance, and the survival rate in the seedling stage is obviously improved; on the contrary, gene editing strains and EMS mutants with deletion of functions are more sensitive to salt stress, and the survival rate is remarkably reduced. The ZmARR2 is an important positive regulatory factor in plant salt tolerance, and the research result provides valuable gene resources and theoretical basis for further analyzing a plant salt tolerance molecular mechanism and developing a new breeding strategy for improving the plant salt tolerance, and has important significance for guaranteeing seedling formation of crops in a salinization region. And a new research direction is provided for finally improving the yield stability.

The application relates to the technical field of bamboo fiber biological degumming, and provides an application process of a low-energy-consumption temperature-sensitive enzyme preparation for bamboo fiber degumming. The process adopts a temperature-sensitive enzyme preparation containing pectinase, hemicellulase, xylanase and a temperature-sensitive response regulator, carries out a degumming reaction on bamboo fiber under low-temperature conditions of 30-40 DEG C, makes the enzyme preparation maintain high enzyme activity to realize efficient degumming, when the reaction reaches a predetermined degree, the temperature is increased to not less than 50 DEG C to trigger the phase transition of the temperature-sensitive response regulator, the enzyme preparation is rapidly inactivated, and therefore the degumming reaction can be controllably terminated. The process avoids the problems of high energy consumption and fiber damage caused by traditional medium-high-temperature enzyme methods or long-time reaction, can ensure the degumming effect and fiber performance stability, and can control the energy consumption of unit mass bamboo fiber at a low level.

The application provides a tomato SlRRB9 gene and application of a knockout vector of the tomato SlRRB9 gene in tomatoes. The gene number of the tomato response regulator gene SlRRB9 is LOC101258668, and the nucleotide sequence is shown as SEQ ID NO. 1. The knockout vector of the tomato response regulator gene SlRRB9 and an engineering bacterium comprising the knockout vector, the knockout vector targets an sgRNA-1 sequence shown as SEQ ID NO. 2 and an sgRNA-2 sequence shown as SEQ ID NO. 3. The application is selected from 1) and / or 2): 1) increasing the fruit size of tomatoes; 2) increasing the fruit weight of tomatoes. The application can obtain a tomato plant with increased fruit size by CRISPR / Cas9 gene editing of the SlRRB9 gene, which is of great significance for promoting high yield of tomatoes and reducing breeding costs.

The invention discloses a genetically engineered bacterium for producing rhamnolipid and application of the genetically engineered bacterium, and belongs to the technical field of microorganisms and genetic engineering. According to the invention, a Psl polysaccharide coding gene cluster and a Pel polysaccharide coding gene cluster of Pseudomonas aeruginosa AB93066 (starting strain) are knocked out, a coding gene FleQ of a cyclic diguanylate receptor is further knocked out, and a response regulation factor BfmS is over-expressed, so that the genetically engineered bacterium for producing rhamnolipid is obtained. The strain has the advantages of short fermentation period, strong rhamnolipid synthesis capability and weak biofilm formation capability, can effectively improve the production efficiency of rhamnolipid and reduce the biosafety risk, and is suitable for industrial production and application.