4 results about "Response regulator" patented technology

The application discloses an Escherichia coli genetically engineered strain for producing pyruvic acid derivative compounds, and discloses a method for improving the yield of pyruvic acid derivative compounds in the Escherichia coli, and discloses the application of the Escherichia coli genetically engineered strain in biosynthesis of poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene. The application can widely enhance the synthetic metabolic flow with pyruvic acid as a key node by knocking out a leucine response regulator gene, and the modification can effectively strengthen the synthesis capacity of the engineered strain for the four target products, i.e., poly-3-hydroxybutyric acid lactate, 3-hydroxypropionic acid, free fatty acid and lycopene under the condition of various carbon sources. Moreover, the knockout of the gene can specifically promote the lactic acid precursor flux, thereby improving the molar proportion of lactic acid monomers in the product P(3HB-LA). lrp co ​​

The invention provides an application of a cytokinin response regulatory factor ZmARR2 gene and an encoding protein thereof in regulating plant salt tolerance, and belongs to the field of plant genetic engineering breeding. The invention discloses the key effect of ZmARR2 in regulating and controlling the salt tolerance of corn for the first time, and compared with a wild type, a ZmARR2 overexpression strain shows stronger salt stress tolerance, and the survival rate in the seedling stage is obviously improved; on the contrary, gene editing strains and EMS mutants with deletion of functions are more sensitive to salt stress, and the survival rate is remarkably reduced. The ZmARR2 is an important positive regulatory factor in plant salt tolerance, and the research result provides valuable gene resources and theoretical basis for further analyzing a plant salt tolerance molecular mechanism and developing a new breeding strategy for improving the plant salt tolerance, and has important significance for guaranteeing seedling formation of crops in a salinization region. And a new research direction is provided for finally improving the yield stability.

The application relates to the technical field of bamboo fiber biological degumming, and provides an application process of a low-energy-consumption temperature-sensitive enzyme preparation for bamboo fiber degumming. The process adopts a temperature-sensitive enzyme preparation containing pectinase, hemicellulase, xylanase and a temperature-sensitive response regulator, carries out a degumming reaction on bamboo fiber under low-temperature conditions of 30-40 DEG C, makes the enzyme preparation maintain high enzyme activity to realize efficient degumming, when the reaction reaches a predetermined degree, the temperature is increased to not less than 50 DEG C to trigger the phase transition of the temperature-sensitive response regulator, the enzyme preparation is rapidly inactivated, and therefore the degumming reaction can be controllably terminated. The process avoids the problems of high energy consumption and fiber damage caused by traditional medium-high-temperature enzyme methods or long-time reaction, can ensure the degumming effect and fiber performance stability, and can control the energy consumption of unit mass bamboo fiber at a low level.

The application provides a tomato SlRRB9 gene and application of a knockout vector of the tomato SlRRB9 gene in tomatoes. The gene number of the tomato response regulator gene SlRRB9 is LOC101258668, and the nucleotide sequence is shown as SEQ ID NO. 1. The knockout vector of the tomato response regulator gene SlRRB9 and an engineering bacterium comprising the knockout vector, the knockout vector targets an sgRNA-1 sequence shown as SEQ ID NO. 2 and an sgRNA-2 sequence shown as SEQ ID NO. 3. The application is selected from 1) and / or 2): 1) increasing the fruit size of tomatoes; 2) increasing the fruit weight of tomatoes. The application can obtain a tomato plant with increased fruit size by CRISPR / Cas9 gene editing of the SlRRB9 gene, which is of great significance for promoting high yield of tomatoes and reducing breeding costs.