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Grape cytokinin response regulator VvRR gene and encoded protein and application thereof

A technology of grape cells and regulatory factors, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of reducing fruit setting rate, affecting fruit setting and young fruit development, abnormal seed development, etc., and achieving the goal of increasing fruit setting rate Effect

Active Publication Date: 2019-08-02
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GID1s mutation affects fruit setting and young fruit development, gid1a fruit setting rate is significantly reduced, gid1b and gid1c fruit size changes, and seed development is abnormal
Map-based cloning found that the fwf site contains an ARF8 gene, which leads to the inability to initiate the production of fruit and seed development signals, and acts as a repressor to prevent the development of carpels, thereby reducing the fruit set rate

Method used

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  • Grape cytokinin response regulator VvRR gene and encoded protein and application thereof
  • Grape cytokinin response regulator VvRR gene and encoded protein and application thereof
  • Grape cytokinin response regulator VvRR gene and encoded protein and application thereof

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Embodiment 1

[0033] The nucleotide sequence of the grape cytokinin response regulator VvRR gene in this example is shown in SEQ ID NO.1.

[0034] The cloning of the grape cytokinin response regulator VvRR gene in this embodiment comprises the following steps:

[0035] (1) Design of 5'RACE primers: 5'RACE primers VvRR5'RACE-R were designed according to the partial sequence obtained by transcriptome sequencing, wherein:

[0036] VvNAC5'RACE-R: 5'-TTCTTCTGCTCCTTCTTCTTAAGC-3' (shown in SEQ ID NO.3).

[0037] (2) Extract the total RNA in the young fruit before and after fruit setting of 'Jingxiu' grapes, according to TaKaRa company RACE5'Kit instructions for reverse transcription;

[0038] (3) 5' RACE technology to clone the full length of VvRR gene: according to TaKaRa company The RACE 5'Kit manual was used for PCR reaction, in which the forward primer was UPM and the reverse primer was VvRR5'RACE-R, and the reaction system and reaction procedures were carried out according to the kit m...

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Abstract

The invention relates to a grape cytokinin response regulator VvRR gene and an encoded protein and application thereof, and belongs to the technical field of plant genetic engineering. The invention utilizes the transgenic technology of a driving principle by a strong promoter to transfer an over-expression vector of the VvRR gene into a tomato, thereby obtaining transgenic tomato plants. Experimental results show that, compared with wild-type tomato plants, the fruit setting rate of transgenic tomato plants with over-expressed VvRR gene is significantly increased. This indicates that the VvRRgene is closely related to the fruit setting rate, and therefore, the VvRR gene may be used to improve the fruit setting rate of plants.

Description

technical field [0001] The invention relates to a grape cytokinin response regulator VvRR gene, its encoded protein and its application, and belongs to the technical field of plant genetic engineering. Background technique [0002] Grapes and wine are rich in minerals, vitamins and various essential amino acids that are beneficial to the human body. Among them, resveratrol has the effects of anti-cancer, anti-inflammation, anti-aging and improving immunity, which has attracted worldwide attention and promoted the development of the world's grape industry. American grapes or European and American grape hybrids have excellent fruit quality and strong stress resistance, and occupy a dominant position in the table grape industry. However, American grapes or European and American hybrid grapes show the characteristics of low fruit setting rate, and a large amount of plant growth regulators are used in the production process to promote grape fruit setting. The use of plant growt...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/08A01H6/82
CPCC07K14/415C12N15/8261
Inventor 余义和郭大龙孟祥轩李旭飞杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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