The invention relates to the technical field of preservatives, in particular to a preservative for prolonging the shelf life of grapes or fresh cut flowers and a preparation method and a use method thereof. Raw materials include water, cane sugar, pectin, hydrophilic resin, abscisic acid, gibberellin and a plant antibacterial agent. The preservative for prolonging the shelf life of grapes or fresh cut flowers has a good fresh-keeping effect, can prolong the shelf life of table grapes by 3-5 days, and can prolong the flowering phase of the fresh cut flowers by 2-3 days compared with in-vitro fresh flowers; meanwhile, a portable capsule-shaped fresh-keeping bottle can be conveniently arranged on fresh grape stems or fresh cut flower branches, and the preservative for prolonging the shelf life of grapes or fresh cut flowers is in a jelly form, so that the preservative for prolonging the shelf life of grapes or fresh cut flowers does not leak no matter how the portable capsule-shaped fresh-keeping bottle is placed during transportation. The preservative for prolonging the shelf life of grapes or fresh cut flowers can replenish moisture and nutrition for fresh grapes or fresh cut flowers without causing waste.