Microbial time temperature indicator used for table grape quality perception

A time-temperature, fresh-table grape technology is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., to achieve low production cost, favorable for large-scale production, and simple method. Effect

Active Publication Date: 2015-10-07
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Lactobacillus paracasei has not been used to make microbial TTI

Method used

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  • Microbial time temperature indicator used for table grape quality perception
  • Microbial time temperature indicator used for table grape quality perception
  • Microbial time temperature indicator used for table grape quality perception

Examples

Experimental program
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Embodiment 1

[0035] Preparation of Lactobacillus paracasei bacterial liquid: activate the dry powder of Lactobacillus paracasei twice, then culture at 37°C for 24 hours, centrifuge the bacterial liquid, wash twice with sterile normal saline to obtain a precipitate, add sterile normal saline , prepared to a concentration of 10 -8 ~10 -9 CFU / ml microbial bacterial solution, for later use.

[0036] Preparation of growth substrate: preparation of MRS broth.

[0037] Prepare the MRS solution mixed with the pH indicator: add 0.04 g of bromocresol green powder per liter of MRS broth.

[0038] The microbial bacterium liquid and the MRS solution mixed with the pH indicator have a volume ratio of 10 -5 : 1 mix evenly, divide into container, promptly make microbial TTI.

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Abstract

The present invention provides a microbial time temperature indicator used for table grape quality perception, which includes a container, the container contains an evenly-mixed microorganism microbial broth, a growth medium and a pH indicator; the microorganism is Lactobacillus paracasei bacilli; the growth medium is MRS bouillon culture-medium; and the pH indicator is bromocresol green. The activation energy of the time temperature indicator is close to the activation energy of fruits and vegetables, can be used to monitor quality changes of the fresh fruits and vegetables in cold chain logistics, and in particular, can be used for accurate quality perception and monitor of table grapes.

Description

technical field [0001] The invention relates to the technical field of time-temperature-shelf life indication and quality monitoring, in particular to a microbial time-temperature indicator for quality perception of table grapes. Background technique [0002] Time Temperature Indicator (TTI) is a kind of time and temperature history that can record and track the fresh agricultural product logistics process, and directly reflect all or part of the shelf life of the monitored product through mechanical deformation, color change or color movement. Identification and perception technology. The monitoring results are more reliable than the traditional preset shelf life, the latest sale date and other labels, ensuring consumer safety. According to the working principle, TTI can be divided into chemical type, enzyme type, microbial type, physicochemical type, diffusion type, polymer type, solid-state reaction type, and light-sensitive TTI. Past studies mainly focused on enzymatic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 张小栓孙格格肖新清刘苑睿朱志强
Owner CHINA AGRI UNIV
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