The invention discloses a preparation and application method of an
enzyme type
capsule time temperature indicator, and relates to the technical field of detection.The preparation and application method comprises the steps that first, first
linear regression equations under different
dilution multiples are established, and then reaction endpoint time under the
dilution multiples is calculated; calculating a standard original
enzyme activity unit and standard reaction time, and establishing a second
linear regression equation under different
dilution multiples by taking the
enzyme activity unit as an independent variable and the logarithm of
absorbance as a dependent variable; adopting a 100 [mu] L capillary tube,
sintering one end of the capillary tube by using an
alcohol blast burner, adding the enzyme liquid with the dilution multiple by using a microsyringe after cooling, clamping the part filled with the enzyme liquid by using a frozen clamp, and
sintering the other end of the capillary tube by using the
alcohol blast burner to obtain the enzyme type
capsule time temperature indicator. An enzyme type
capsule time temperature indicator is used for calculating accumulated heat in the whole heat
sterilization process, the heat sterilization intensity is evaluated, and the experiment cost and time are saved.