553 results about "Lactobacillus paracasei" patented technology

The invention provides a Lactobacillus paracasei, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The lactobacillus paracasei ID001 provided by the invention has an inhibition effect on enteropathogenic bacteria such as salmonella, escherichia coli, staphylococcus aureus and the like. The probiotic preparation is obtained through fermentation and the dendrobium officinale extract, and the probiotic preparation has the immunoregulation effect through performance verification and screening.

The invention relates to the technical field of microorganisms, in particular to a viable bacterium preparation of a lactobacillus paracasei strain as well as a preparation method and application of the viable bacterium preparation. The viable bacterium preparation comprises a lactobacillus paracasei strain and auxiliary materials, and the preservation number of the lactobacillus paracasei strain is CGMCC (China General Microbiological Culture Collection Center) No.27974. The viable bacterium preparation can inhibit the growth of streptococcus mutans, reduce the formation of a dental plaque biofilm caused by the streptococcus mutans, reduce the content of a mature dental plaque biofilm, inhibit the level of water-insoluble exopolysaccharides generated by the streptococcus mutans, reduce the expression quantity of cariogenic genes gtfB, gtfC, gtfD, ftf and LuxS of the streptococcus mutans, improve the cariogenic activity of the streptococcus mutans and improve the cariogenic activity of the streptococcus mutans. The adhesion rate of streptococcus mutans on a hydroxyapatite tooth model is reduced, and a beneficial in-vitro probiotic function is achieved.

The invention relates to the technical field of oral cavity cleaning and nursing materials, and discloses an oral cavity nursing composition containing plant extract components, which comprises plant extract components, inactivated probiotics, prebiotics and fluoride. The preparation method of the toothpaste product comprises the following steps: step 1, weighing corresponding components according to a formula, adding the cortex magnoliae officinalis extract, the lactobacillus paracasei, the fructo-oligosaccharide, the sucralose, the sodium benzoate and the sodium fluoride into the deionized water, stirring to fully dissolve, then adding the glycerol and the sorbitol, and stirring uniformly to obtain a mixture 1; fully mixing silicon dioxide, cellulose gum, hydroxyethyl cellulose and sodium lauryl sulfate to obtain a mixture 2; compared with the prior art, the oral cavity cariogenic bacteria inhibiting composition has the advantages that various oral cavity cariogenic bacteria are inhibited safely and effectively by using plant extract components, and beneficial bacteria proliferation is promoted by inactivating probiotics agglutinating harmful bacteria and prebiotics, occupation in oral mucosa is increased, colonization of harmful bacteria is reduced, so that the anti-sugar effect is achieved, and caries are prevented.

The invention relates to a preparation method of a tricholoma matsutake fermentation product with anti-wrinkle and firming effects as well as a product and application of the tricholoma matsutake fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, a carbon source, inorganic salt and water, then sterilizing to obtain a sterilized culture medium, inoculating a composite probiotic agent into the medium, and carrying out fermentation culture to obtain the tricholoma matsutake fermentation product with anti-wrinkle and firming effects. Centrifuging, filtering and sterilizing the culture product to obtain a tricholoma matsutake fermentation product; the bacterial strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, a lactobacillus paracasei YK-L003 bacterial strain and a saccharomyces cerevisiae YK-S001 bacterial strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of the three strains has an excellent skin conditioning effect, can effectively improve the skin state and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-wrinkle cosmetics.

The invention discloses a lactobacillus paracasei SX-M1559 strain and application thereof in preparation of drugs for reducing blood sugar and lipid and resisting inflammation, and belongs to the technical field of microorganisms. The invention discloses a lactobacillus paracasei SX-M1559, and the preservation number of the lactobacillus paracasei SX-M1559 is CCTCC (China Center For Type Culture Collection) NO: M 2025030. According to the lactobacillus paracasei SX-M1559 and the application thereof in preparation of the medicine for reducing blood sugar, blood fat and inflammation, the SX-M1559 is separated from fresh feces of long-life old people, teai county, Zhanjiang city, Guangdong province, the glucose content in a zebra fish hyperglycemia model body and the fat content in a zebra fish hyperlipidemia model body can be remarkably reduced, and the effect of reducing blood sugar, blood fat and inflammation is achieved. The invention provides a theoretical reference and a guidance basis for developing a probiotic preparation for preventing or treating hyperlipidemia, diabetes mellitus and inflammatory bowel disease by utilizing the new strain SX-M1559 of the casei paracasei.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei ZY and application thereof, and the preservation number of the lactobacillus paracasei ZY is CGMCC (China General Microbiological Culture Collection Center) No.30303. The invention provides the lactobacillus paracasei ZY capable of regulating the iron balance of intestinal tracts, and the lactobacillus paracasei ZY also can promote the growth of saccharomyces cerevisiae. The animal feed prepared from the lactobacillus paracasei ZY can regulate the iron absorption of intestinal tracts according to the iron content in blood, promote the intestinal tracts to autonomously regulate the iron balance, and effectively solve the problem of iron balance imbalance in large-scale production; a fermented product prepared from the lactobacillus paracasei ZY is good in palatability, digestion is promoted, and the immunity of the organism is enhanced.

The invention discloses a Lactobacillus paracasei strain with a uric acid reducing function and application thereof, the Lactobacillus paracasei strain is named as Lactobacillus paracasei C125, and is preserved in China Center for Type Culture Collection on February 17, 2025, the preservation number is CCTCC M 2025231, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The fermentation supernatant of the casei paracasei has the functions of reducing uric acid, resisting oxidation and inhibiting bacteria, and has a wide application prospect.

The invention discloses a composition for treating insomnia. Comprising probiotic compounds: lactobacillus plantarum, bifidobacterium lactis, lactobacillus crispatus, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus reuteri, lactobacillus casei, lactobacillus bulgaricus, lactobacillus fermentum, lactobacillus gasseri, lactococcus lactis, bifidobacterium longum, lactobacillus salivarius, bifidobacterium breve, streptococcus thermophilus and lactobacillus paracasei; lactobacillus helveticus; and traditional Chinese medicine components and prebiotics. Probiotics are matched with traditional Chinese medicines with homology of medicine and food, and corresponding prebiotics are supplemented, so that the balance of intestinal flora can be adjusted and information transmission between intestines and cranial nerves can be promoted through the synergistic effect of the probiotics and the corresponding prebiotics, and then gastrointestinal inflammation, ulcer, helicobacter pylori infection, constipation and insomnia are treated or improved; and treating or improving spleen and stomach disharmony, liver qi stagnation, excessive heart fire, fire excess from yin deficiency, heart-kidney disharmony caused by emotion, pressure, diseases and the like, and insomnia caused by the symptoms.

The present invention discloses a probiotic postbiotic composition for improving the scalp environment, a preparation method thereof and an application thereof, wherein the probiotic postbiotic composition for improving the scalp environment comprises postbiotics obtained by fermenting two strains of Lactobacillus paracasei ProSci-101 and Lactobacillus fermentum ProSci-602; the Lactobacillus paracasei ProSci-101 has a preservation number of CGMCC No.26528; the Lactobacillus fermentum ProSci-602 has a preservation number of CGMCC NO.25451. The probiotic postbiotic composition for improving the scalp environment of the present invention has the effects of cell repair, enhancing elastin and renewing the metabolism of the dermis, can slow down cell aging, and promote the proliferation of stratum corneum cells; thereby effectively achieving the purpose of improving the scalp environment and preventing hair loss.

The invention discloses a probiotic composition for relieving acute drunkenness as well as a preparation method and application of the probiotic composition, belongs to the technical field of probiotic application, and provides a probiotic composition for relieving acute drunkenness, and is characterized in that the probiotic composition comprises lactobacillus paracasei STB and lactobacillus reuteri Cre360, the lactobacillus paracasei STB comprises lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus reuteri Cre360, lactobacillus paracasei, lactobacillus The preservation number of the lactobacillus paracasei STB is CCTCC (China Center For Type Culture Collection) NO: M2022881; the preservation number of the lactobacillus reuteri Crea360 is CCTCC (China Center for Type Culture Collection) NO: M2025682. The probiotic combination group is formed by combining lactobacillus paracasei STB and lactobacillus reuteri mucus Cre360, so that the symptom of acute drunkenness can be effectively relieved, and the probiotic combination group has the effects of resisting oxidation and resisting inflammation.

The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus paracasei strain and application thereof. The invention discloses a Lactobacillus paracasei strain, the name of the Lactobacillus paracasei strain is Lactobacillus paracasei WG-Lpc 63, the Lactobacillus paracasei strain is preserved in China General Microbiological Culture Collection Center on August 27th, 2024, and the preservation number is CGMCC No. 31768. The Lactobacillus paracasei strain is named as Lactobacillus paracasei WG-Lpc 63, and the Lactobacillus paracasei strain is named as Lactobacillus paracasei WG-Lpc 63. The strain disclosed by the invention has good acid production capacity, cholate tolerance and intestinal tract adhesion capacity, and has the effects of regulating intestinal flora balance and relieving intestinal inflammation.

The invention provides a halite-containing probiotic preparation for improving gastrointestinal environment and a preparation method, the halite-containing probiotic preparation comprises the following components in parts by weight: 60-70 parts of a probiotic composition, the probiotic composition comprises lactobacillus casei Zhang, animal bifidobacterium subsp. Lactis V9, lactobacillus plantarum P-01, animal bifidobacterium subsp. Lactis B-01 and casei paracasei LC-11, the weight ratio is 3: 2: 2: 1: 1. 1.2 to 3.5 parts of nanoscale refined halite; 10 to 15 parts of a microcapsule embedding material; 0.6 to 1.2 parts of an antioxidant system; 15 to 30 parts of auxiliary materials; the probiotic preparation disclosed by the invention has the characteristics of obvious strain combination advantage, nano-scale halite protection and synergism, accurate release of a multi-layer microcapsule structure, synergistic effect of an antioxidant system and the like, the survival rate of probiotics, gastrointestinal tract tolerance and intestinal colonization capacity are remarkably improved, and a new technical scheme is provided for improving gastrointestinal health.

The invention discloses a strain of Lactobacillus paracasei SX-G1520 and an application of the Lactobacillus paracasei SX-G1520 in preparation of a lipid-lowering, anti-inflammatory and / or antioxidant product, the preservation number of the Lactobacillus paracasei SX-G1520 is CCTCC (China Center for Type Culture Collection) No: M 2025032, and the Lactobacillus paracasei SX-G1520 is preserved in the China Center for Type Culture Collection on January 6, 2025. The Lactobacillus paracasei SX-G1520 strain provided by the invention has lipid-lowering and antioxidant functions, significantly reduces the content of intestinal neutrophils of a zebra fish inflammatory bowel disease model, shows a significant effect on relieving intestinal inflammation, is good in safety, and has good application prospects. And a new technical choice is provided for developing probiotic preparations for regulating fat metabolism, resisting oxidative stress and preventing and treating inflammatory bowel diseases.

The present invention relates to the technical field of fermented Chinese medicine products, and discloses a fermented Chinese medicine product for regulating spleen and stomach function and a preparation method thereof. The fermented Chinese medicine product is prepared by a Chinese medicine extract, a fermentation medium and Lactobacillus paracasei LBR-002 of the same origin of medicine and food, and the mass ratio of the Chinese medicine extract, the fermentation medium and Lactobacillus paracasei LBR-002 is 1000:965:20-90, and the Chinese medicine extract comprises the following components in parts by weight: 25-60 parts of yam, 20-50 parts of liquorice, 20-45 parts of ganoderma lucidum, 20-42 parts of tuckahoe, 20-50 parts of polygonatum, 20-48 parts of codonopsis, 18-25 parts of dendrobium officinale, 8-17 parts of daidaihua, 8-16 parts of American ginseng, 5-20 parts of coix seeds, 6-15 parts of amomum, and 8-20 parts of hawthorn. The fermented Chinese medicine product has the characteristics of effective regulation, green safety and the like to spleen and stomach function, and can significantly improve spleen and stomach function.

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention relates to the technical field of microorganisms and food biology, and provides a lactobacillus paracasei and tagatose composition capable of synergistically treating functional constipation, the active ingredients of the composition are lactobacillus paracasei ZYhyy-004 and tagatose, the lactobacillus paracasei is named as ZYhyy-004, the tagatose is named as ZYhyy-004, the tagatose is named as ZYhyy-004, and the tagatose is named as ZYhyy-004. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the classification name of the strain is Lacticaseibacillus paracasei, the preservation number of the strain is CGMCC No.31554, and the preservation date of the strain is August 5, 2024. Experiments prove that in the composition of the lactobacillus paracasei and the tagatose, the lactobacillus paracasei ZYhyy-004 and the tagatose have a synergistic effect in treating and / or improving the functional constipation of animals, and can be used for improving or treating the functional constipation.

The invention relates to a Lactobacillus paracasei LC97, and an application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus paracasei LC97 for relieving allergy is CGMCC (China General Microbiological Culture Collection Center) NO. 32421. Based on the strain, the invention also provides application and related products of the strain. The LC97 strain has broad-spectrum antibacterial performance, has the functions of enhancing immune function and relieving atopic dermatitis, and can relieve atopic dermatitis by relieving skin lesion, inhibiting inflammation, reducing ear swelling degree and regulating cell factor level. And by reducing the total levels of serum IgE and IL-4, the levels of IFN-gamma and IL-10 are improved, and the levels of cytokines are regulated, so that the immunity is enhanced.

The invention relates to the technical field of intelligent food production, in particular to a probiotic plant beverage production method and system.The method comprises the steps that mildewed and damaged areas of penthorum chinense pursh, gastrodia elata and other plant raw materials are recognized and removed, the content of reducing sugar is detected, and a traceability identifier is generated; the preparation method comprises the following steps: compounding lactobacillus plantarum A, lactobacillus plantarum B and lactobacillus paracasei, embedding by adopting a sporopollen-calcium alginate core-shell structure, and adjusting the thickness of a shell layer and the concentration of carboxymethyl pachymaran; correcting Arrhenius equation activation energy Ea through bacterial colony density data so as to calculate the attenuation rate k value in the shelf life; after the fermentation liquor is embedded for the second time, QR code labels are generated for products qualified in primary inspection, and RFID temperature and humidity sensing chips are integrated; and pushing real-time shelf lives of qualified finished products, probiotic flora activity reports and traceability identifiers to consumers. According to the invention, the problems of low efficiency, lag in fermentation regulation, backward traceability technology and isolated intelligent technology of the traditional process are solved.

The invention discloses a Lactobacillus paracasei metagen with a constipation relieving effect and application thereof, and belongs to the field of microorganisms. The lactobacillus paracasei CCFM1452 provided by the invention is relatively good in activity, and has certain acid and alkali resistance and adhesion. According to the present invention, with the application of the lactobacillus paracasei CCFM1452, the inflammation level in the colon tissue can be significantly reduced, the pathological damage of the colon tissue can be improved, the levels of the proinflammatory factor and the anti-inflammatory factor can be adjusted, the aquaporin expression level can be reduced, and the motilin MTL content in the colon tissue can be increased; the content of vasoactive intestinal peptide VIP in the colon tissue is reduced; the compound has the advantages of enhancing intestinal tract movement, shortening intestinal tract transfer time and regulating intestinal tract steady state, and has a huge application prospect in preparation of medicines for relieving constipation.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention belongs to the field of microorganisms, and particularly relates to application of lactobacillus paracasei L9 in preparation of a preparation for improving pulmonary fibrosis of an aged mouse. According to the lactobacillus paracasei L9 disclosed by the invention, the synthesis of I-type collagen is reduced by regulating and controlling gene expression, so that pulmonary fibrosis is relieved; meanwhile, the L9 improves the content of propionic acid and butyric acid in intestinal tracts and serum by regulating the abundance of SCFA-producing microbial flora in the intestinal tracts, so that pulmonary fibrosis is inhibited, and the L9 is a potential ideal preparation for improving or treating aging-related pulmonary fibrosis.

The invention discloses a Lactobacillus paracasei grx06 and an application thereof in the aspects of dispelling the effects of alcohol and protecting the liver, the strain number of the Lactobacillus paracasei is grx06, the Lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center on February 18, 2025, and the preservation number is CGMCC No: 33560. The lactobacillus paracasei obtained by screening in the invention has the capability of producing ethanol dehydrogenase and acetaldehyde dehydrogenase at high yield, and is high in ethanol tolerance. In addition, the traditional Chinese medicine composition has the effects of promoting ethanol metabolism in rats and recovering liver injury. Wide application prospects are realized in the field of anti-alcohol and liver-protecting products.

The present invention provides a novel antioxidant Lactiplantibacillus paracasei TDM-2, its cell-free extract and applications, belonging to the technical field of lactic acid bacteria. The Lactiplantibacillus paracasei TDM-2 provided by the present invention is deposited in the China General Microbiological Culture Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is February 20, 2023, and the deposit number is CGMCC NO. 26579. The Lactiplantibacillus paracasei TDM-2 and its cell-free extract provided by the present invention have good scavenging rates of DPPH free radicals and tolerance to H2O2, and also have good reducing ability and scavenging ability for OH ‑ ions.

The invention belongs to the technical field of probiotic particles, and particularly relates to probiotic particles for improving intestinal functions as well as a preparation method and application of the probiotic particles. The probiotic particles capable of improving the intestinal function comprise a probiotic core, a first embedding layer, a second embedding layer and a third embedding layer, the probiotic inner core is prepared from plant lactobacillus, bifidobacterium animalis, lactobacillus acidophilus, bifidobacterium longum, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus fermentum, lactobacillus helveticus, streptococcus thermophilus, plant lactobacillus, lactobacillus helveticus and lactobacillus rhamnosus. According to the present invention, the twelve probiotics are contained, and the latest three-layer embedding technology is adopted so as to enhance the storage property and the digestive tract tolerance, such that the product can maintain the beneficial bacterium activity for a long time without refrigeration, and the product is suitable for the crowd with the alteration of the intestinal flora.

The invention relates to a compound microorganism deodorization fungicide as well as a preparation method and application thereof, and belongs to the technical field of microorganism application. The invention provides a compound microorganism deodorization bacterium agent. The compound microorganism deodorization bacterium agent is prepared from bacillus cereus GXMU-Q5.3, streptomyces beihaiensis GXMU-J15 and lactobacillus paracasei GXMU-G1, the viable count ratio of the bacillus cereus GXMU-Q5.3 to the streptomyces beihaiensis GXMU-J15 to the lactobacillus paracasei GXMU-G1 is (1 to 2): (1 to 2): (1 to 2). The strain of the composite microbial deodorant agent is screened from soil and water at the stacking place of the marine product processing waste, the decomposition and deodorization effects on the marine product processing waste are obvious, the bacteria have a synergistic effect, and the deodorization effect of the composite microbial agent is improved by 40% or above compared with that of a single strain; the cost is low, the efficiency is high, the application range is wide, the deodorization period is short, and the safety is high.

The invention provides application of lactobacillus paracasei IOB413 and a metagen thereof in preparation of a medicine for improving radioactive hematopoietic injury, and relates to the field of microorganisms. The lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center on June 29, 2018, and the preservation number of the lactobacillus paracasei IOB413 is CGMCC (China General Microbiological Culture Collection Center) No.16022. The lactobacillus paracasei IOB413 has the advantages that the lactobacillus paracasei IOB413 is preserved in the China General Microbiological Culture Collection Center; the lactobacillus paracasei IOB413 and the metaplasm thereof can improve the damage condition of immune organs, increase the number of lymphocytes, increase the number of red blood cells and blood platelets and recover the immune function and hematopoietic function of the organism, so that the radioactive hematopoietic damage is improved.

The invention discloses a method for quantitatively detecting viable bacteria of lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus acidophilus. The novel molecular detection target disclosed by the invention has high specificity on target bacteria. The disclosed PMA-qPCR method comprises the steps of target detection, qPCR primer and probe design, qPCR detection system, PMA treatment condition and viable bacteria quantitative standard curve. After qPCR amplification, if a Cq value appears, the dissolution peak is single and the dissolution temperature is consistent with that of a positive sample (only aiming at a dye method), the lactic acid bacteria are identified as target lactic acid bacteria, and the number of viable bacteria can be calculated according to a corresponding standard curve. The method can effectively detect and count viable bacteria of Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei and Lactobacillus acidophilus, has the advantages of rapidness, accuracy, simple operation and the like, and is convenient for food enterprises and inspection institutions to use.

The invention relates to the technical field of microorganisms, in particular to a probiotic composition for improving sleep and application, and the probiotic composition comprises lactobacillus paracasei BMC-2020, lactobacillus plantarum MCA-3066, lactobacillus rhamnosus, lactobacillus fermentum and lactobacillus casei. The probiotic composition provided by the invention is safe and efficient, has a prospect of developing a new medicine for improving sleep, and has a clinical practical value; the probiotic composition is used for fermenting the traditional Chinese medicine composition, the obtained traditional Chinese medicine fermentation composition contains probiotics and traditional Chinese medicine fermentation liquor, the probiotics and the traditional Chinese medicine fermentation liquor can effectively achieve a synergistic effect, and the sleep improving efficacy of a traditional Chinese medicine fermentation product is promoted; the traditional Chinese medicine fermented composition can reduce the awakening activity amount and the total awakening time, and has the effect of improving sleep.

The present invention relates to novel lactic acid bacteria, Bifidobacterium bifidum P61 KCCM13367P, Lactobacillus paracasei P62 KCCM13368P, or a mixture thereof. The novel lactic acid bacteria and the mixture thereof of the present invention change the composition of intestinal microorganisms to inhibit the expression of sarcopenia markers (MuRF-1, MAFbx / Atrogin-1, etc.), increase muscle strength and muscle mass, and promote muscle formation, thereby alleviating sarcopenia. In addition, the present invention improves cognitive dysfunction by inhibiting behavior similar to cognitive dysfunction and regulating the expression of factors (BDNF, IL-10, etc.) associated with said dysfunction. In addition, the present invention has the effect of treating inflammation by reducing the expression of inflammatory markers (TNF-α, IL-6, IL-1ß, and MPO) and increasing the expression of an anti-inflammatory marker (IL-10).