64results about How to "Improve colonization rate" patented technology

The invention discloses a method for rapid mycorrhization of a DSE strain and blueberry tissue culture seedlings, and belongs to the technical field of biology. A strain is R16, and the preservation number is CGMCC No. 13885. The invention also establishes the method for the rapid mycorrhization of the strain and the blueberry tissue culture seedlings. The system uses sterilized moss as a co-culture medium under an aseptic condition, and DSE inoculant adopts a liquid culture method. Compared with an existing method of inoculation by fungus lumps, the strain R16 cultivated by inoculated liquid grows fast and contacts with blueberry root fungus fully, the co-culture time is short and the planting rate is high. The DSE strain R16 has a significantly promoting effect on the growth of the blueberry tissue culture seedlings. Compared with the uninoculated control, the growth of the blueberry seedlings inoculated with DSE strain is significant, the leaves are large and dark green, the root system is developed and the survival rate of transplanting is high.

The invention relates to a high-concentration microbial inoculum containing polyglutamic acid and a preparation method and application of the high-concentration microbial inoculum. The high-concentration microbial inoculum containing polyglutamic acid is prepared from mixed microbial fermentation powder, light calcium carbonate, polyglutamic acid, magnesium sulfate, boric acid and zinc sulfate. Polyglutamic acid is added in the microbial inoculum, since polyglutamic acid contains a large number of super hydrophilic groups, namely, carboxyl groups, therefore, the microbial inoculum can keep the soil moisture, improve the porosity and bulking intensity of the soil, improve the water-retention capacity and fertilizer-rentention capacity of the soil and enhance the phosphorus releasing ability and the potassium releasing ability, and at the same time, the microbial colonization rate can be greatly improved by adding polyglutamic acid in the microbial inoculum.

Provided is a method for reducing the cadmium content in tobacco leaves. The method comprises the specific steps that (1) nanometer material zinc oxide is used for replacing ZnSO4.7H2O in original Kafer culture media, and 10 mg of nanometer material zinc oxide is added into 1 liter of culture media; (2) activated Piriformospora indica is inoculated to the liquid Kafer culture media in the step (1) for culture, then mycelia are collected, Piriformospora indica mycelium liquid is made and poured to roots of tobacco seedlings, and root-irrigation is conducted so that the Piriformospora indica can be colonized to the roots of the tobacco seedlings; (3) the tobacco seedlings are transplanted after the Piriformospora indica is colonized, and conventional water and fertilizer management are conducted so that the tobacco seedlings can grow normally. The growth status of the tobacco seedlings can be improved in the method, the amount of cadmium moving to overground parts of the tobacco seedlings from root systems is reduced, heavy metal residue on the tobacco leaves is reduced, and the method is easy to master, low in cost, simple, practical and environmentally friendly, and can not case secondary pollution.

The invention discloses saline-alkaline tolerant enterobacter cloacae and a production method and application of a viable bacterium preparation of the saline-alkaline tolerant enterobacter cloacae. The saline-alkaline tolerant enterobacter cloacae ZTS-10 is separated from rhizosphere soil of chili peppers in saline-alkaline land, the preservation number of the bacterial strain in the China GeneralMicrobiological Culture Collection Center (CGMCC) is CGMCC No.20181, and the preservation date is 3 July, 2020. The bacterial strain is high in saline-alkaline tolerance and high in field planting rate in the saline-alkaline soil, has the capacity of producing IAA, degrading phosphorus and degrading potassium, can promote growth of the chili peppers, has good antagonism effects on pathogenic bacteria of chili pepper epidemic diseases, can be used for producing microorganism fertilizers especially microorganism fertilizers special for the chili peppers in the saline-alkaline land, can reduce the generation of chili pepper epidemic diseases, and can increase yield and quality.

The invention provides an economical and environment-friendly vine felt for cultivation of eel grass. Environmentally friendly plastic in a viscous flow state is processed into three-dimensional circular filament vine felt with the customizable thickness, wherein multiple irregular filaments are staggered disorderly and bound together at the contact points. The vine felt is an elastic planting bedwith very low apparent density and can overcome the limitation of climate and hydrological conditions in the existing cultivation technology of eel grass, and eel grass plants can planted into the cultivation holes indoors. Under the condition of high transparency water in deep winter or early spring, large-scale, large-area, economical and fast throwing and planting of eel grass is carried out without draining water. The method has great practical values in water and soil conservation, aquatic ecological restoration and construction, ecological aquaculture, ornamental waterscape pools and water cluster boxes in China.

The invention discloses a probiotics solid beverage containing mushroom polysaccharides. The probiotics solid beverage containing mushroom polysaccharides is characterized by comprising the following components in percentages by weight: pleurotus eryngii polysaccharide 3-4.5%, tremella polysaccharide 4.5-5%, probiotics powder 4-6%, oligomeric galactose 10-15%, oligofructose 5-10%, water-soluble dietary fibers 60-70% and sucralose 0.05-0.1%.

The invention discloses a direct field-returning and decomposition promoting method for rice stubble and wheat straw in an area along Huaihe river, which comprises the following operating steps: (1) straw smashing and direct returning to a field; (2) application of a base fertilizer and C/N ratio regulation; (3) application of a straw decomposition agent and field-planting promoting with degradingbacteria; (4) soil rotary tillage and irrigation; and (5) transplanting of seedlings, topdressing and activation of degrading bacterium activity. The invention provides the direct field-returning anddecomposition promoting method for the rice stubble and wheat straw in the area along Huaihe river, the method has good effects of returning the straw to the field and promoting decomposition and isbeneficial to the accumulation of soil nitrogen and the increase of the crop yield.

The invention provides a method for reducing cadmium content of an overground part of paddy rice. The method comprises the following specific steps: (1) by use of utilizing nano material ZnO to replace ZnSO4.7H2O in an original Kafer culture medium, adding 10mg of nano material ZnO into per litre of the culture medium; (2) inoculating the activated piriformospora indica into the liquid Kafer culture medium obtained in the step (1) for culturing, then collecting mycelia, preparing a piriformospora indica mycelium solution, then pouring the piriformospora indica mycelium solution to the root of a paddy rice seedling, and performing root-irrigation treatment so as to colonize the piriformospora indica at the root of the paddy rice seedling; (3) after performing piriformospora indica colonization, transplanting the paddy rice into a large field, and then performing routine water and fertilizer management so as to enable the paddy rice to grow normally. The method is easy to master, relatively low in cost, simple and practical, environment-friendly, incapable of causing secondary pollution, applicable to production of chemical-free rice, not only applicable to planting of rice in heavy metal contaminated soil, and also applicable to uncontaminated soil.

The invention relates to the technical field of agricultural seedling raising, in particular to a degradable seedling raising pot for breeding rhizophora apiculata. The seedling raising pot is specially designed for a rhizophora apiculata growth environment, and comprises a seedling raising pot body, a base and an anchoring portion. In order to facilitate a root system to penetrate sand during planting with the pot body, an air hole is formed in the bottom of the seedling raising pot; in order to further fasten nursery stocks and prevent the same from lodging and rotting or being flushed awayby sweater, the seedling raising pot is provided with the anchoring portion and the base; the anchoring portion is arranged at the mouth of the pot and can rotate, the base is connected with the pot body through a connecting rod, and two ends of the base can be demounted. The degradable seedling raising pot is integrally made from a decomposable organic material, the pot body does not need to be taken out during planting, convenience, fastness and environment protection are achieved, the survival rate after planting is high, and the work efficiency is improved.

The invention discloses saline-alkaline-tolerant Bacillus aerius and a preparation method and application of a living bacteria preparation of the saline-alkaline-tolerant Bacillus aerius. According tothe invention, the saline-alkaline tolerant Bacillus aerius PAPM-14 is separated from rhizosphere soil of corn. The strain is preserved in China General Microbiological Culture Collection Center on June 22, 2020, and the preservation number of the strain is CGMCC No. 20118. The strain has strong salt and alkali tolerance; the strain has strong IAA production, phosphate solubilization and potassium solubilization capabilities, has a strong antagonistic effect on pathogenic bacteria such as corn sheath blight disease, can be used for producing microbial fertilizers, especially microbial fertilizers special for saline-alkali soil corn, is used for biological prevention and control of crop diseases such as corn sheath blight disease, reduces the occurrence of diseases, and improves the yieldand quality.

The invention discloses a preparation method of a dark septate endomycorrhiza fungal inoculant combination. The preparation method specifically comprises the steps of plant root system selecting and processing, cultivating system preparation, isolation and culture of strains, purifying culture of the strains, preparation and fermental cultivation of seed solutions of the strains, biocontrol bacteria powder preparation, and preparation of dark septate endomycorrhiza fungal inoculant combination. The dark septate endomycorrhiza fungal inoculant combination prepared by the invention has the advantages that the inoculant combination is good in quality, high in infection rate, high in colonization frequency, long in expiration date, and stable in infection effect, the preparation method can bewidely applied to the fields of scientific research, ecological control, soil improvement and restoration and the like, large-scale factory operation is achieved, and the industry requirement is met.

The invention discloses a preparation method of an apple tree canker antibiological inoculant. The method comprises the steps that apple tree canker pathogen hyphae can be directly adopted as nutrition for growth and breeding, apple tree canker pathogen hyphae can be induced to produce various exocellular enzymes for degrading pathogen cell walls, streptomyces albidoflavus Actin-1 colonized at apple tree canker scab for a long time is activated to produce spores, a spore suspension is prepared, then, the spore suspension is inoculated into a liquid seed culture medium to be fermented to obtain a fermentation seed solution, finally, the fermentation seed solution is inoculated into a solid fermentation culture medium for solid fermentation to obtain spore raw powder, and then, the spore raw powder, an ultraviolet protectant, a dispersing agent, a carrier and other auxiliaries are scientifically screened and compounded to obtain the antibiological inoculant with the multiple advantages of being good in control efficiency, high in efficiency, low in recurrence rate, high in environment adaptability, high in stability, not likely to generate drug resistance and the like; the antibiological inoculant is the first choice for biological control, and is of great significance in improving the control effect of garden stuff pathogenic bacteria such as apple tree canker, preventing pathogenic bacterium relapse and achieving environmental protection.

The invention relates to the technical field of medical preparations and processes, and particularly discloses an intestinal flora capsule as well as a preparation method and application thereof. Through excrement collection, flora extraction, preparation preparation, preparation filling and other technologies, intestinal flora is prepared into capsules in the form of freeze-dried powder, intestinal flora transplantation is performed by taking the capsules by a human body, and compared with an existing method, the method has the advantages of being safe, convenient, high in flora colonizationrate and the like. A preparation auxiliary liquid used during preparation of the intestinal flora capsule can effectively protect intestinal flora, reduce the influence of excrement on flora composition, improve the survival rate (more than 90%) of the intestinal flora in freeze-dried powder, and improve the colonization rate of the flora in intestinal tracts, and the obtained capsule can be stably stored in an environment of subzero 20 DEG C for a long time and is convenient for patients to take in daily life, and a new effective intestinal flora transplanting method is provided for maintaining the daily good mental state of the human body.

The invention provides an application of lactobacillus rhamnosus LRa05 in preparation of a preparation for improving irritable bowel syndrome. The lactobacillus rhamnosus LRa05 freeze-dried powder provided by the invention has a remarkable curative effect on the irritable bowel syndrome, and can be used for reducing serum endotoxin, reducing proinflammatory factors, increasing the level of anti-inflammatory factors, relieving oxidative stress and adjusting intestinal flora disorder, so that the intestinal flora is healthy. The lactobacillus rhamnosum LRa05 freeze-dried powder product provided by the invention is simple to prepare and suitable for large-scale production, has the characteristics of simple treatment, high remission rate, no toxic or side effect and the like as an auxiliary medicine of IBS, and is relatively high in practicability.

The invention belongs to the technical field of daily health care of livestock and poultry, and particularly discloses a device and a method for preparation of lactobacillus acid water and the application of the lactobacillus acid water. The method for the preparation of the lactobacillus acid water includes hydrolysis of waste residue of agricultural and sideline products, activation of lactobacillus liquid, lactobacillus fermentation of the waste residue of the agricultural and sideline products, and solid-liquid separation for preparing the lactobacillus acid water. Compared with acid water which is prepared through inorganic acid and organic acid or composite acid in the prior art, the lactobacillus acid water prepared through the device and the method for the preparation of the lactobacillus acid water not only can regulate digestive systems and gastrointestinal acid-base environments of the livestock and the poultry, but also has a large quantity of living lactic acid bacteria, immune systems of breeding pigs can be effectively improved, and the morbidity and the incidence rate of diarrhea are lowered. In addition, the device and the method for the preparation of the lactobacillus acid water are simple, high in operability and low in production cost, can effectively utilize the waste residue resources of the agricultural and sideline products, enable the waste residue of the agricultural and sideline products to be further degraded, and solve the prominent problems, such as waste of resources and environmental pollution, to a certain extent.