Seedling culture fungicide for improving colonization rate and infection intensity of rice arbuscular mycorrhizal fungi and application of seedling culture fungicide

Abstract

The invention discloses a seedling culture fungicide for improving the colonization rate and the infection intensity of rice arbuscular mycorrhizal fungi and application, relates to the field of microorganisms, and aims to improve the colonization rate and the infection intensity of the arbuscular mycorrhizal fungi on the rice and solve the problems that the colonization rate and the infection intensity are greatly influenced by the soil environment at present, and the arbuscular mycorrhizal fungi fiercely compete with indigenous microorganisms. The invention provides a novel seedling raising method for improving the colonization rate and the infection intensity of the arbuscular mycorrhizal fungi of the rice. According to the method, a culture substrate is conventional soil; the seedling raising method is easy to operate, infection can be conducted in the rice seedling stage, and then field transplanting is conducted; the culture mode is simple, efficient and low in cost; the addition amount of the selenite is strictly controlled within a reasonable selenium-rich soil content range, no adverse effect is caused to the growth environment, and meanwhile the selenium content in rice seedlings is increased.

Description

Technical Field

[0001] The invention belongs to the field of agriculture, and in particular relates to a seedling-growing bacterial agent for improving the colonization rate and infection intensity of rice arbuscular mycorrhizal fungi and an application thereof. Background Art

[0002] Rice is one of the most important food crops in the world, but its growth is often adversely affected by its inability to adapt to changes in the external environment. Arbuscular mycorrhizal fungi (AMF) are widely distributed soil microorganisms, accounting for more than 10% of the soil microbial biomass. They can form a symbiotic relationship with rice, which is essential for the growth and survival of rice under adverse conditions. On the one hand, AMF can directly promote rice growth by optimizing the nutritional status of rice. On the other hand, AMF can provide indirect growth support for rice by improving soil structure and enhancing rice's stress resistance. In order to enable rice to better adapt to various living environments and increase rice yields, the formation of a symbiotic system between rice and arbuscular mycorrhizal fungi is particularly important. However, the current colonization rate of arbuscular mycorrhizal fungi (AMF) on rice is limited. This phenomenon may be attributed to the compatibility issues between rice and AMF, or it may be restricted and affected by specific environmental factors.

[0003] The soil environment has a significant impact on the infection effect of AMF. Physical and chemical properties such as soil pH, organic matter content, available phosphorus and alkaline nitrogen will affect the growth and activity of AMF, which will further affect its infection effect on rice. In addition, AMF will also compete with indigenous microorganisms, affecting the colonization rate and infection intensity of AMF. In summary, the effective way to improve the colonization rate and infection intensity of AMF is to accurately control soil conditions so that the nutritional conditions of the soil are coordinated with the AMF ratio.

[0004] Publication No. CN110028361A, invention name "A planting method for promoting organic selenium enrichment of rice by using mycorrhizal fungi" provides a method for applying sand rich in Glomus selenium during rice planting; the sand rich in Glomus selenium is composed of the following substances by weight: 1-3 parts of sodium selenite, 1-2 parts of Glomus selenium, 20-30 parts of silkworm litter, 30-40 parts of straw powder and 20-30 parts of diatomaceous earth. The planting method proposed by the present invention can use Glomus selenium to It provides effective selenium fertilizer for rice. It can use Glomus mosseae to convert sodium selenite, which is difficult to absorb, into organic selenium fertilizer, which is easy to absorb, to provide selenium for rice. Diatomaceous earth can improve the water retention performance of sand rich in Glomus mosseae fungi and can effectively extend the life of Glomus mosseae. Ultimately, Glomus mosseae can provide sufficient organic selenium fertilizer for rice, and diatomaceous earth can enrich the organic selenium fertilizer and then release it slowly after enrichment to ensure that the selenium element can be fully absorbed and improve the utilization rate of the selenium element.

[0005] Although this patent uses a combination of sodium selenite and Glomus mosseae, it does not study the effect of sodium selenite on the colonization rate of Glomus mosseae. Summary of the invention

[0006] The purpose of the present invention is to provide a new seedling raising method for improving the colonization rate and infection intensity of rice arbuscular mycorrhizal fungi, so as to solve the problems that the colonization rate and infection intensity of AMF-infected rice are greatly affected by the soil environment, the competition with indigenous microorganisms is fierce, and the operation is difficult.

[0007] The invention discloses a seedling cultivation microbial agent for improving the colonization rate and infection strength of rice arbuscular mycorrhizal fungi. The microbial agent is composed of Pipepogon monsesiae and selenite. The Pipepogon monsesiae is Pipepogon monsesiae with a preservation number of CGMCC No.3012.

[0008] Furthermore, the selenite content in the bacterial agent is 0.1-1.0 mg / kg, and the spore amount of Moses's spores is 60-70 / g.

[0009] The invention discloses an application of a bacterial agent for raising seedlings for improving the colonization rate and infection intensity of arbuscular mycorrhizal fungi of rice, which is used for improving the colonization rate and infection intensity of arbuscular mycorrhizal fungi of rice root system.

[0010] Furthermore, the rice root system is a rice seedling root system.

[0011] Furthermore, the method of increasing the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the roots of rice seedlings is specifically as follows:

[0012] The rice seeds after germination treatment are sown in a culture medium, and then a bacterial agent is added and cultured to the rice seedling stage, thereby completing the improvement of the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the root system of the rice seedlings.

[0013] Furthermore, the germination treatment is to germinate the seeds until one-third of the seeds are white and then sow them in the culture medium.

[0014] The present invention has the following beneficial effects:

[0015] The arbuscular mycorrhizal fungi used in the present invention is Glomus mosseae, numbered: HDSF1, and deposited: CGMCC No.3012. The Glomus mosseae fungi selected in the present invention is different from other arbuscular mycorrhizal fungi in that the fungus species can efficiently infect plant roots and form a huge mycelium network system, thereby more effectively promoting the absorption and utilization of nutrients by plants. After the fungus is synergistically applied with selenite, the colonization rate of the roots of rice seedlings is significantly improved, effectively promoting the growth and development of plants.

[0016] The method of the present invention has the advantages of high colonization efficiency and small application amount of bacterial agent and selenium salt. After colonization is completed, the biomass of rice seedlings is significantly increased.

[0017] The present invention uses easily accessible soil as a substrate, is easy to operate, and can be easily transplanted to the field after colonization in a small-scale seedling tray. By accurately controlling the application amount of the microbial agent and selenium salt, the dual advantages of low risk and low cost are obtained. The present invention not only effectively improves the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the root system of rice seedlings by applying an appropriate amount of selenite (0.5 mg / kg), but also properly solves the related problems currently faced by rice selenium enrichment.

[0018] The culture substrate of the method of the present invention is conventional soil; the seedling raising method is simple to operate, and the rice seedlings can be infected first, and then transplanted into the field; the culture mode is simple, efficient and low-cost. In addition, the content of selenite added by the present invention can be accurately controlled within the content range of reasonable selenium-rich soil, has no adverse effect on the growth environment of rice, and at the same time increases the selenium content in the rice seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a diagram of the experimental device used in the method of the present invention to cultivate seedlings; Figure 1 (a) Inoculation and addition of selenite (0.5 mg / kg), (b) Inoculation but no addition of selenite;

[0020] Figure 2 This is a microscopic observation picture of rice after being infected by the method of the present invention; Figure 2 (a) Inoculation with bacteria and addition of selenite (0.5 mg / kg), (b) Inoculation without addition of selenite. DETAILED DESCRIPTION

[0021] In order to make the objectives, technical solutions and advantages of the embodiments of the present invention more clearly understood, the spirit of the contents disclosed by the present invention will be described in detail below. After understanding the embodiments of the contents of the present invention, any technician in the relevant technical field can change and modify them according to the techniques taught by the contents of the present invention without departing from the spirit and scope of the contents of the present invention.

[0022] The exemplary embodiments of the present invention and the description thereof are used to explain the present invention but are not intended to limit the present invention.

[0023] Example 1: In 2024, rice planting was carried out in the Key Laboratory of Cold Region Ecological Restoration and Resource Utilization of Heilongjiang University:

[0024] 1. Uncontaminated farmland plough layer soil (0-20 cm) was collected from the Hulan Campus of Heilongjiang University (longitude 126°25′, latitude 45°49′) as the test matrix;

[0025] 2. The rice seeds were surface sterilized with 3% (v / v) hydrogen peroxide for 30 min, then rinsed with deionized water, and then germinated on moist filter paper at 20°C to complete the rice seed treatment;

[0026] 3. Sow the treated rice seeds in a seedling tray filled with the test substrate, the seedling tray has an upper diameter of 65 mm, a lower diameter of 28 mm, and a depth of 65 mm, and sow 3 seeds per pot. Add arbuscular mycorrhizal fungi (Glomus mosseae, accession number: CGMCC No. 3012) inoculant (5% (v / v)) and sodium selenite (0.5 mg / kg) to the culture substrate at the same time; the spore count of Glomus mosseae is 67 spores / g.

[0027] 4. Laboratory management: On the 30th day of cultivation, the rice seedlings were taken out, the roots were cleaned, and the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the roots of rice seedlings were determined by trypan blue staining.

[0028] The colonization rate and infection intensity of arbuscular mycorrhizal fungi on the roots of rice seedlings were tested, and the results are shown in Table 1.

[0029] Table 1

[0030]

[0031]

[0032] Through statistical analysis of multiple sets of sampling data, after adding selenite, the colonization rate of rice seedlings reached 82.2%, which is 1.16 times higher than that without adding selenite, and the infection intensity reached the highest level. At this time, the amount of selenite added is within the content range of selenium-rich soil, which is environmentally friendly and risk-free, and the selenium content in the stems of rice seedlings is also significantly improved.

[0033] In order to further investigate the cooperative effect of selenite and Pipepophysalis mosseae, the colonization rate and infection intensity of arbuscular mycorrhizal fungi on the roots of rice seedlings at different concentrations of selenite were investigated, as shown in Table 2. As can be seen from Table 2, the colonization rate does not increase with the increase of selenite concentration. The present invention found that the addition of 0.1-1.0 mg / kg of selenite can achieve the ideal colonization rate and infection intensity. At this concentration, the combination of selenite and Pipepophysalis mosseae can improve the colonization rate and infection intensity. The addition of 0.5 mg / kg of selenite has the highest colonization rate and infection intensity.

[0034] Table 2

[0035]

[0036] Note: Inoculation refers to Glomus mosseae, accession number: CGMCC No.3012.

[0037] Infection intensity: +(0-10); ++(10-20); +++(20-30); ++++(30-40); +++++(40-50).

Claims

1. A seedling-growing inoculant for improving the colonization rate and infection intensity of rice arbuscular mycorrhizal fungi, characterized in that The bacterial agent is composed of Pipestilens mosseae and selenite; the Pipestilens mosseae is Pipestilens mosseae with a preservation number of CGMCC No.3012.

2. A seedling-growing bacterial agent for improving the colonization rate and infection intensity of rice arbuscular mycorrhizal fungi according to claim 1, characterized in that The selenite content in the bacterial agent is 0.1-1.0 mg / kg, and the spore quantity of the moses spore is 60-70 spores / g.

3. The use of a seedling-growing inoculant for improving the colonization rate and infection intensity of rice arbuscular mycorrhizal fungi as claimed in claim 1, characterized in that The bacterial agent is used for improving the colonization rate and infection intensity of arbuscular mycorrhizal fungi in rice root systems.

4. The use according to claim 3, characterized in that The rice root system is a rice seedling root system.

5. The use according to claim 3 or 4, characterized in that The method for improving the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the root system of rice seedlings is specifically as follows: The rice seeds after germination treatment are sown in a culture medium, and then a bacterial agent is added and cultured to the rice seedling stage, thereby completing the improvement of the colonization rate and infection intensity of arbuscular mycorrhizal fungi in the root system of the rice seedlings.

6. The use according to claim 5, characterized in that The germination treatment is to germinate the seeds until one third of the leaves are white and then sow them in the culture medium.

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