Composition with anti-hair loss effect as well as application and cosmetic thereof

Abstract

The invention belongs to the field of cosmetics, and discloses a composition with an anti-hair-loss effect and application thereof.The composition is prepared by mixing a cacumen biotae extract, a radix polygoni multiflori extract, an oil control factor and soybean milk fermentation liquor, and the anti-hair-loss effect of the cacumen biotae extract and the radix polygoni multiflori extract is further enhanced through the oil control factor and the soybean milk fermentation liquor; in addition, the invention further discloses a cosmetic which contains 1-10 wt% of the composition.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular to a composition having anti-hair loss efficacy, its use, and cosmetics. Background Art

[0002] Normally, a person has about 100,000 to 150,000 hairs, which have different growth cycles and usually grow or fall out during three stages: growth phase, regression phase, and resting phase. Generally, alopecia refers to an abnormal increase in the number of hairs that fall out due to a shortening of the hair growth rate during the growth phase and an increase in the number of hairs that fall out during the regression phase and resting phase.

[0003] The causes of hair loss known in the art that have been explored may include different influencing factors, such as age, season, physiological factors and diseases, as well as various causes including hyperandrogenism, excessive sebum secretion, deterioration of scalp function due to peroxides and bacteria, genetic factors, aging, stress, etc.

[0004] It is well known that scalp diseases such as dandruff and seborrheic dermatitis accompany the early stages of hair loss as typical early symptoms. Specifically, it is known that scalp inflammation is caused by the following factors: physical factors include, for example, skin hypersensitivity to various pollutants or bacteria, excessive sebum production due to endocrine system dysfunction, autonomic nerve disorders, or circulatory system dysfunction such as blood circulation disorder, nutrient deficiency of hair follicles, allergies, bacterial infections, etc.; genetic factors such as hormone effects; environmental factors include, for example, various pollutions, processed foods as food mainstream, shampoo, soap, mousse, hair gel, hair spray, perm, dyeing, hair dryers, detergents, etc., all of which may jointly affect.

[0005] Chinese patent application 202210335912.9 discloses a drug formula for preventing hair loss and promoting hair growth, a microneedle patch and a preparation method thereof, wherein the drug is composed of the following ingredients: 1-8 parts of Platycladus orientalis leaf extract, 0.2-3 parts of Ligusticum chuanxiong extract, 0.2-4 parts of black tea extract, 0.1-2 parts of clove bud extract, 0.17-2.5 parts of Eclipta prostrata extract, 0.17-2 parts of Cuscuta seed extract, 0.5-5 parts of Ligustrum lucidum fruit extract, 0.5-5.5 parts of Sanguisorba officinalis root extract, and safflower extract. 0.1-2 parts, ginseng extract 0.1-2.5 parts, rehmannia root extract 0.1-1.5 parts, star anise fruit extract 0.1-2 parts, dandelion extract 0.2-3 parts, Polygonum multiflorum extract 0.3-2.5 parts, perilla leaf extract 0.1-3 parts, purslane extract 0.2-2 parts, black mulberry fruit extract 0.1-1.5 parts, ginger root extract 0.1-3 parts, angelica root extract 0.5-8 parts, salvia miltiorrhiza extract 0.1-2 parts, ginkgo leaf extract 0.5-5 parts.

[0006] This program starts with the differentiation of etiology and pathogenesis of traditional Chinese medicine, and the prescription is complete with the main, auxiliary, and guiding ingredients. It has the effects of regulating the liver and kidney, nourishing blood and activating blood circulation, dispelling wind and unblocking meridians, removing dampness and promoting qi, etc. It can stimulate the regeneration of hair follicles, dredge meridians, and stimulate the circulation of qi and blood, thereby achieving the effect of preventing hair loss and promoting hair growth.

[0007] Chinese patent application 202410970071.8 discloses an anti-hair loss composition, consisting of the following ingredients: 3-6 parts of ginger root extract, 2-5 parts of Cladosiphon marinum extract, 3-5 parts of silkworm extract, 3-5 parts of Platycladus orientalis leaf extract, 1-3 parts of ginseng extract, 3-6 parts of Polygonum multiflorum extract, and 0.5-2.6 parts of Ligusticum chuanxiong extract.

[0008] This solution uses ethyl acetate to extract ginger roots. The resulting ginger root extract greatly retains the original volatile oils, gingerol, curcumin, gingerol, protein, volatile aroma components, and multiple vitamins and minerals. Adding Cladosiphon sea star extract in combination with silkworm extract and ginger root extract can effectively promote the scalp's absorption of its active ingredients. The three together have a significant synergistic effect.

[0009] It is not difficult to see from the above two schemes that Platycladus orientalis leaves, Polygonum multiflorum and ginger root have good beneficial effects in treating and preventing hair loss.

[0010] The problem that this solution needs to solve: How to improve the anti-hair loss effect of Platycladus orientalis leaf extract and Polygonum multiflorum extract. Summary of the invention

[0011] To solve the above problems, the present application provides a composition with anti-hair loss efficacy, its use, and cosmetics. By combining oil control factors with Platycladus orientalis leaf extract and Polygonum multiflorum extract, hair loss can be effectively prevented. By combining it with soy milk fermentation liquid, the anti-hair loss effect of the composition can be further enhanced.

[0012] To achieve the above purpose, the present application discloses a composition with anti-hair loss effect, wherein the composition is prepared by mixing Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil control factor, and the mass ratio of Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil control factor is 5.8-6.2: 0.4-0.6: 0.001-0.1;

[0013] Among them, Platycladus orientalis leaf extract can effectively repair and protect the scalp by promoting the activity of hair follicles, reducing the production of scalp oil, and improving the scalp environment, thereby achieving the purpose of preventing hair loss; Polygonum multiflorum extract can also inhibit scalp inflammation and bacterial growth, while also increasing the blood circulation of the scalp, regulating the metabolism of the scalp, thereby providing more oxygen and nutrients to the hair follicles and reducing hair loss; the oil-control factor can effectively regulate the division and proliferation of sebaceous cells and inhibit the activity of 5α-reductase, thereby reducing the sebum secretion of the scalp, and reducing the damage of free radicals to the hair, protecting the hair from damage, thereby helping to reduce hair loss.

[0014] Further preferably, the composition further comprises soybean milk fermentation liquid, and the mass ratio of soybean milk fermentation liquid to Platycladus orientalis leaf extract is 0.001-0.1:5.8-6.2;

[0015] The protein, vitamins, minerals and other nutrients contained in fermented soy milk further promote the growth of hair. Fermented soy milk can also reduce the damage of free radicals to hair, protect hair from environmental influences, and maintain hair health.

[0016] Preferably, the method for preparing the soymilk fermentation liquid specifically comprises the following steps:

[0017] Step 1: Mix soybeans with water, heat, squeeze, sterilize, and filter to obtain soy milk;

[0018] Step 2: adding the fermentation liquid to soybean milk for fermentation, sterilization, and filtering to obtain a fermentation filtrate, and then mixing the fermentation filtrate with water, butanediol, and 1,2-pentanediol to obtain soybean milk fermentation liquid;

[0019] In step 1, the mass ratio of soybean to water is 1:8-10;

[0020] In step 2, the mass ratio of the fermentation liquid to the soy milk is 1:4-6;

[0021] The fermentation broth in step 2 is Lactobacillus bulgaricus fermentation broth, which is obtained by activating Lactobacillus bulgaricus through an activation medium;

[0022] The activation medium contains 2% by mass of glucose, 2% by mass of protein, 2% by mass of yeast extract, 1% by mass of disodium hydrogen phosphate and 1% by mass of Lactobacillus bulgaricus, and the balance is water;

[0023] In step 2, the mass ratio of the fermentation filtrate to water, butanediol, and 1,2-pentanediol is 9-11:76-80:9-11:1-3;

[0024] Further preferably, the mass ratio of the fermentation filtrate to water, butanediol and 1,2-pentanediol is 10:78:10:2.

[0025] In addition, the invention also discloses the use of the composition for preparing cosmetics.

[0026] In addition, a cosmetic is disclosed, which contains 1-10 wt % of the composition.

[0027] Preferably, the cosmetic further contains water, a skin conditioner, a surfactant, a moisturizer, a cooling agent, and a preservative;

[0028] The mass ratio of water, composition, skin conditioner, surfactant, moisturizer, cooling agent and preservative is 60:6-7:27.5-28.5:0.8-1:3-5:0.4-0.6:0.2-0.3.

[0029] Preferably, the skin conditioning agent is selected from at least one of ethanol, adenosine, salicylic acid, niacinamide, dextrin, caffeine, cyclodextrin, allantoin, ginseng root fermentation product filtrate, hydrolyzed ginsenosides, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, angelica root extract, liquorice root extract, Centella asiatica extract, Cladosiphon marinum extract, and decapeptide-4;

[0030] The surfactant is selected from at least one of PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, and PEG-35 castor oil;

[0031] The moisturizing agent is selected from at least one of glycerin, sodium polyaspartate, 1,2-pentanediol, panthenol, 1,3-propylene glycol, and butylene glycol;

[0032] The cooling agent is selected from at least one of menthol and menthol lactate;

[0033] The preservative is selected from at least one of 1,2-hexanediol, phenoxyethanol, ethylhexylglycerin and chlorphenesin.

[0034] Preferably, the cosmetic is an emulsion, an essence, a spray, a shampoo or a hair mask.

[0035] The beneficial effects of this application are:

[0036] The present application provides a composition with anti-hair loss efficacy, which is combined with Platycladus orientalis leaf extract and oil-control factor to effectively target the oil secretion of the scalp, inhibit the activity of 5α-reductase, and thereby reduce scalp inflammation and cell growth, and then increase the blood circulation of the scalp through the use of Polygonum multiflorum extract, regulate the metabolism of the scalp, and provide more oxygen and nutrients for the hair follicles. The three synergistically enhance the effect and reduce hair loss; and combined with soy milk fermentation liquid to further promote hair growth, while also reducing the damage to the hair by free radicals, protecting the hair from environmental influences, and maintaining the health of the hair. DETAILED DESCRIPTION

[0037] In the description of this application, it should be noted that if the specific conditions are not specified in the examples, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially.

[0038] Product information:

[0039] Platycladus orientalis leaf extract: provided by Shanghai Huiwen Biotechnology Co., Ltd.;

[0040] Polygonum multiflorum extract: provided by Guangzhou Baoyi Biotechnology Co., Ltd.;

[0041] Oil control factor: provided by Croda Chemicals (Shanghai) Co., Ltd., wherein the oil control factor is composed of Enanthophyllum tomato bark extract, dianthin, and butylene glycol, and the mass ratio of Enanthophyllum tomato bark extract, dianthin, and butylene glycol is 5:0.03:94.97;

[0042] Ginger root extract: provided by Guangzhou Meiyi Biotechnology Co., Ltd.;

[0043] Angelica extract: provided by Beijing Dongfang Miaosen Biotechnology Co., Ltd.;

[0044] Green flower Ennan tomato bark extract: provided by Shanghai Yuyu New Material Technology Co., Ltd., effective substance content: 99%;

[0045] Caryophyllein: provided by Xi'an Hengji Chemical Co., Ltd., product number: HJDT011;

[0046] Lactobacillus bulgaricus: provided by Shaanxi Good Luck Pharmaceutical Technology Co., Ltd., with a Lactobacillus bulgaricus content of 10 billion cfu / g;

[0047] Peptone: provided by Jinan Qingyuyuan New Materials Co., Ltd.;

[0048] Yeast extract: provided by Xi'an Binghe Biotechnology Co., Ltd.

[0049] Unless otherwise specified, the preparation method of the soy milk fermentation liquid in the embodiments and comparative examples is as follows: soybeans and water are mixed in a mass ratio of 1:9, heated to 60°C, squeezed, sterilized, and filtered to obtain soy milk; then the bacterial liquid of Lactobacillus bulgaricus and soy milk are mixed in a mass ratio of 1:5, fermented at 37°C for 48h, sterilized, and filtered to obtain a fermentation filtrate, and then the fermentation filtrate is mixed with water, butanediol, and 1,2-pentanediol in a mass ratio of 10:78:10:2 to obtain a soy milk fermentation liquid;

[0050] The bacterial liquid of Lactobacillus bulgaricus is prepared by adding Lactobacillus bulgaricus to an activation medium and fermenting at a constant temperature of 28°C for 24 hours, wherein the activation medium contains 2% by mass of glucose, 2% by mass of protein, 2% by mass of yeast extract, 1% by mass of disodium hydrogen phosphate and 1% by mass of Lactobacillus bulgaricus, and the balance is water.

[0051] Example 1

[0052] The Platycladus orientalis leaf extract, Polygonum multiflorum extract and oil control factor are mixed in a mass ratio of 6:0.5:0.01, and stirred to obtain a composition with anti-hair loss effect.

[0053] Example 2

[0054] The Platycladus orientalis leaf extract, the Polygonum multiflorum extract and the oil control factor are mixed in a mass ratio of 6.2:0.4:0.1, and stirred to obtain a composition with anti-hair loss effect.

[0055] Example 3

[0056] The Platycladus orientalis leaf extract, the Polygonum multiflorum extract and the oil control factor are mixed in a mass ratio of 5.8:0.6:0.001, and stirred to obtain a composition with anti-hair loss effect.

[0057] Example 4

[0058] The method is basically the same as Example 1, except that the Platycladus orientalis leaf extract, Polygonum multiflorum extract, oil control factor, and soybean milk fermentation liquid are mixed and stirred at a mass ratio of 6:0.5:0.01:0.01 to obtain a composition with anti-hair loss effect.

[0059] Example 5

[0060] The method is basically the same as Example 1, except that the Platycladus orientalis leaf extract, Polygonum multiflorum extract, oil control factor, and soybean milk fermentation liquid are mixed and stirred at a mass ratio of 6:0.5:0.01:0.001 to obtain a composition with anti-hair loss effect.

[0061] Example 6

[0062] The method is basically the same as Example 1, except that the Platycladus orientalis leaf extract, Polygonum multiflorum extract, oil control factor, and soybean milk fermentation liquid are mixed and stirred at a mass ratio of 6:0.5:0.01:0.1 to obtain a composition with anti-hair loss effect.

[0063] Example 7

[0064] The method is basically the same as Example 4, except that commercially available bifidobacterium (with a content of 10 billion cfu / g) is used instead of Lactobacillus bulgaricus to be added to the activation medium and fermented at 28°C for 24 hours, wherein the activation medium contains 2% by mass of glucose, 2% by mass of protein, 2% by mass of yeast extract, 1% by mass of disodium hydrogen phosphate and 1% by mass of bifidobacterium, and the remainder is water; finally, a bacterial solution of bifidobacterium is obtained and the bacterial solution of Lactobacillus bulgaricus is replaced with the bacterial solution of the same mass of bifidobacterium and the soy milk is fermented.

[0065] Example 8

[0066] The method is basically the same as Example 4, except that commercially available plant lactobacillus (content is 10,000,000,000 cfu / g) is used to replace Bacillus bulgaricus and add into activation medium for constant temperature fermentation for 24 h at 28 ° C, wherein the activation medium contains 2% glucose by mass, 2% protein by mass, 2% yeast extract by mass, 1% sodium hydrogen phosphate by mass and 1% plant lactobacillus by mass, and the remainder is water; the bacterial liquid of plant lactobacillus is finally obtained and the bacterial liquid of Lactobacillus bulgaricus is replaced by the bacterial liquid of Lactobacillus plantarum of the same mass and soy milk is fermented.

[0067] Example 9

[0068] The method is basically the same as Example 4, except that red beans are used instead of soybeans for fermentation.

[0069] Example 10

[0070] The method is basically the same as Example 4, except that corn is used instead of soybean for fermentation.

[0071] Comparative Example 1

[0072] The method is basically the same as Example 1, except that, with the total mass of the composition remaining unchanged, the composition is composed of a mixture of Platycladus orientalis leaf extract and oil-controlling factor in a mass ratio of 6:0.01.

[0073] Comparative Example 2

[0074] The method is basically the same as Example 1, except that, with the total mass of the composition remaining unchanged, the composition is composed of a mixture of Polygonum multiflorum extract and an oil-controlling factor in a ratio of 0.5:0.01.

[0075] Comparative Example 3

[0076] The method is basically the same as Example 1, except that, with the total mass of the composition remaining unchanged, the composition is composed of a mixture of Platycladus orientalis leaf extract and Polygonum multiflorum extract in a mass ratio of 6:0.5.

[0077] Comparative Example 4

[0078] The method is substantially the same as Example 1, except that ginger root extract is used instead of Platycladus orientalis leaf extract.

[0079] Comparative Example 5

[0080] The method is substantially the same as Example 1, except that the extract of Angelica sinensis is used instead of the extract of Polygonum multiflorum.

[0081] Comparative Example 6

[0082] The method is substantially the same as Example 1, except that Nv. enans tomato bark extract is used instead of the oil control factor.

[0083] Comparative Example 7

[0084] The method is basically the same as Example 1, except that oleanol is used instead of the oil-control factor.

[0085] Application Example 1-17

[0086] A cosmetic is prepared by using the compositions prepared in Examples 1-10 and Comparative Examples 1-7 respectively;

[0087] The preparation method comprises: mixing water, a composition, a skin conditioner, a surfactant, a moisturizer, a cooling agent, and a preservative in a mass ratio of 60:6.5:28:0.9:4:0.4:0.2, dispersing, and obtaining a cosmetic;

[0088] The skin conditioning agent is a mixture of ethanol, niacinamide, allantoin and Centella asiatica extract, and the mass ratio of ethanol, niacinamide, allantoin and Centella asiatica extract is 26.6:0.25:0.15:1;

[0089] The surfactant is PEG-40 hydrogenated castor oil;

[0090] The moisturizer is a mixture of glycerin, butylene glycol and sodium polyaspartate, and the mass ratio of glycerin, butylene glycol and sodium polyaspartate is 2:1:1;

[0091] The cooling agent is menthol;

[0092] The preservative is a mixture of phenoxyethanol and ethylhexylglycerin, and the mass ratio of phenoxyethanol to ethylhexylglycerin is 9:1.

[0093] Application Example 18

[0094] The invention is basically the same as Application Example 4, except that a mixture of licorice root extract and Centella asiatica extract is used in the skin conditioner instead of the Centella asiatica extract, and the mass ratio of the licorice root extract to the Centella asiatica extract is 1:1.

[0095] Application Example 19

[0096] It is basically the same as Application Example 4, except that a mixture of salicylic acid and adenosine is used in the skin conditioner instead of niacinamide, and the mass ratio of salicylic acid to adenosine is 1:1.

[0097] Application Example 20

[0098] It is basically the same as Application Example 4, except that the mass ratio of the water, the composition, the skin conditioner, the surfactant, the moisturizer, the coolant, and the preservative is 60:6.0:28:0.9:4:0.4:0.2.

[0099] Application Example 21

[0100] It is basically the same as Application Example 4, except that the mass ratio of the water, the composition, the skin conditioner, the surfactant, the moisturizer, the cooling agent, and the preservative is 60:7.0:28:0.9:4:0.4:0.2.

[0101] Application Example 22

[0102] It is basically the same as Application Example 4, except that the cosmetic does not contain the composition prepared in Example 4.

[0103] Performance Test:

[0104] 1. Anti-hair loss test

[0105] According to the inclusion criteria, patients with seborrheic alopecia were collected as subjects, aged 23-50 years, with the longest duration of hair loss being 3 years and 2 months, the shortest being 3 months, and the average being 8 months. The patients with seborrheic alopecia were randomly divided into groups, with 10 people in each group, for a total of 22 groups.

[0106] 1. Eligibility criteria:

[0107] (1) Patients with seborrheic alopecia aged 20-50 years old;

[0108] (2) Have not used hormones, immunosuppressants, hair growth drugs or other hair growth cosmetics recently;

[0109] (3) Those who have not participated in other clinical trials and voluntarily participate in this trial and complete the required contents;

[0110] (4) Fill out the informed consent form according to the clinical trial requirements.

[0111] 2. Exclusion conditions:

[0112] (1) Those who do not meet the inclusion criteria;

[0113] (2) Those who have ever been allergic to alcohol preparations or other hair growth products;

[0114] (3) Patients currently suffering from infectious or inflammatory diseases of the head or other skin diseases.

[0115] 3. Test methods:

[0116] The cosmetics obtained in Application Examples 1-22 were evenly applied to the scalp and hair roots, and the scalp was gently massaged until absorbed, twice a day, morning and evening, one month as a course of treatment, and three courses in total. The trial was a double-blind trial. If more than or equal to 7 people in each experimental group had the same therapeutic effect, it was deemed to be the final therapeutic effect of the group. The experimental results are shown in Table 1.

[0117] The criteria for judging the efficacy are as follows: (1) No change: No improvement in oily hair, dandruff, and hair loss, no sprouting of fine hair, and no obvious improvement in appearance;

[0118] (2) Slight improvement: The itching sensation is reduced, dandruff and hair loss are alleviated, the scalp environment is partially improved, and no new hair is seen.

[0119] (3) Significant improvement: Dandruff and itching are reduced, hair loss is reduced, new hair sprouts, excessive sebum secretion is inhibited, appearance is improved, and the scalp environment is effectively improved.

[0120] Table 1

[0121]

[0122]

[0123] Conclusion analysis:

[0124] As can be seen from Table 1, in Application Examples 1-6, when the composition is used to prepare cosmetics, it can significantly improve the hair loss and sebum secretion of patients; observing Application Examples 7-10, when other bacterial species are used to ferment soy milk or other grains are used to replace soybeans for fermentation, the prepared cosmetics can only slightly improve the hair loss of patients; then observing Application Examples 11-13, when only Platycladus orientalis leaf extract and oil control factor, Polygonum multiflorum extract and oil control factor, or Platycladus orientalis leaf extract and Polygonum multiflorum extract are used to prepare the composition, the prepared cosmetics can also only slightly improve the hair loss of patients; observing Application Examples 14-17, when other substances are used to replace Platycladus orientalis leaf extract, Polygonum multiflorum extract or oil control factor, the improvement effect of the cosmetics will change from significant improvement to slight improvement; finally, observing Application Examples 18-22, when the skin conditioner in the cosmetics is replaced or the content of the composition in the cosmetics is changed, the hair loss of patients can also be significantly improved, but when the cosmetics are prepared without adding the composition, there is no change in the hair loss of patients.

[0125] 2. 5α-reductase inhibition rate

[0126] 1 Test Principle

[0127] This test refers to the laboratory method (HMC-WI-0315α-reductase inhibition rate) and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0128] 2 Test indicators

[0129] 5α-reductase inhibition rate determination standard: If the 5α-reductase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0130] 3 Experimental materials and methods

[0131] 3.1 Instruments and Equipment

[0132] BSA224S analytical balance;

[0133] Agilent 1220 high performance liquid chromatograph.

[0134] 3.2 Reagents

[0135] testosterone, 98%;

[0136] NADPH, 90%;

[0137] Anhydrous ethanol.

[0138] 3.3 Test methods

[0139] (1) Treatment of control and test samples

[0140] Sample: The compositions prepared in Examples 1-10 and Comparative Examples 1-7 were diluted with pure water to a concentration of 6.5%, and the cosmetics prepared in Application Examples 4 and Application Examples 18-22;

[0141] Positive control (finasteride, purity ≥98%): dilute with anhydrous ethanol to a positive control concentration of 0.1%;

[0142] Negative control: PBS solution.

[0143] (2) Test operation steps

[0144] A sample group, a positive control group, a negative control group and a blank group were set up. Three parallels were set up for each group. Different reagent solutions were added to the 26 groups respectively, and the solutions were shaken well. Each group was subjected to HPLC analysis to determine the testosterone content in each tube.

[0145] (3) The calculation formula is shown in Formula 1:

[0146]

[0147] (4) Data analysis

[0148] SPSS was used as the statistical analysis software, and the independent sample t-test was used to compare the 5α-reductase inhibition rates among the test samples, positive controls, and negative controls.

[0149] The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 means there is no significant difference between the two groups; P<0.05 means there is a significant difference between the two groups.

[0150] According to the above test method, the anti-hair loss compositions obtained in Examples 1-10 and Comparative Examples 1-7, and the cosmetics prepared in Application Examples 4 and Application Examples 18-22, which were diluted with pure water to a concentration of 6.5%, were tested. The results are shown in Table 2.

[0151] Table 2

[0152]

[0153]

[0154] Conclusion analysis:

[0155] 1. It can be seen from Examples 1-3 that when the ratio of Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil control factor in the composition changes, the inhibition rate of 5α-reductase will also change;

[0156] Furthermore, when observing Example 1 and Example 4, when Platycladus orientalis leaf extract, Polygonum multiflorum extract, oil control factor and soybean milk fermentation liquid form a composition, the inhibition rate of 5α-reductase reaches 48.536%, which is further improved compared with Example 1;

[0157] Further observation of Examples 4-6 shows that as the content of soybean milk fermentation liquid continues to increase, the inhibition rate of the composition on 5α-reductase is also continuously enhanced, but the subsequent enhanced effect will gradually weaken.

[0158] 2. It can be seen from Examples 4 and 7-10 that when soy milk is fermented with bacterial liquid of other strains or the fermented liquid obtained by replacing soybeans with other grains, the inhibitory effect of the formed composition on 5α-reductase is not significantly improved. It can be seen that not all fermented liquids obtained by fermenting soy milk with bacterial liquid of all strains or fermented liquids obtained by fermenting other grains with bacterial liquid of Lactobacillus bulgaricus can be combined with Platycladus orientalis leaf extract, Polygonum multiflorum extract and oil-control factor to achieve the effect of significantly improving the inhibition rate of the composition on 5α-reductase.

[0159] 3. From Example 1 and Comparative Examples 1-3, it can be seen that when the Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil-controlling factor form a composition, the inhibition rate of 5α-reductase can reach 40.265%. From Comparative Examples 1-3, it can be seen that when the Platycladus orientalis leaf extract, Polygonum multiflorum extract, or oil-controlling factor are missing from the composition, the inhibition rate of the composition for 5α-reductase shows a relatively large downward trend;

[0160] Further observation of comparative examples 1-3 shows that the inhibition rate of the composition formed by Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil-controlling factor for 5α-reductase should be 30.592%. However, by observing Example 1, its inhibition rate for 5α-reductase reached 39.265%. It can be seen that there is a synergistic effect between Platycladus orientalis leaf extract, Polygonum multiflorum extract, and oil-controlling factor, which can further enhance the inhibition rate of the composition for 5α-reductase.

[0161] 4. By observing comparative examples 4-7 in combination with Table 2, it can be seen that when ginger root extract and angelica extract are used to replace Platycladus orientalis leaf extract and Polygonum multiflorum extract, respectively, or Enanthophyllum bark extract and dianthus are used to replace the oil-control factor, respectively, the inhibition rate of the obtained composition on 5α-reductase is lower than that in Example 1. It can be seen that Platycladus orientalis leaf extract, Polygonum multiflorum extract and oil-control factor are irreplaceable.

[0162] 5. It can be seen from Example 4 and Application Example 4 that when the composition is used to make cosmetics, the skin conditioner in the cosmetics works together with the composition to increase the inhibition rate of 5α-reductase; and from observing Application Examples 18-19, it can be seen that in the skin conditioner, when a mixture of licorice root extract and Centella asiatica extract is used instead of Centella asiatica extract or a mixture of salicylic acid and adenosine is used instead of niacinamide, the inhibition rate of cosmetics on 5α-reductase can be further increased.

[0163] Further observation of Application Examples 4 and Application Examples 20-21 shows that as the content of the composition increases, the inhibition rate of the cosmetic on 5α-reductase also increases, but when the content increases to a certain level, the increase will gradually decrease and tend to be stable.

[0164] The above embodiments are preferred implementation modes of the present application, but the implementation modes of the present application are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present application shall be equivalent replacement methods and shall be included in the protection scope of the present application.

Claims

1. A composition having an anti-hair loss effect, characterized in that: The composition is prepared by mixing Platycladus orientalis leaf extract, Polygonum multiflorum extract and oil-controlling factor, and the mass ratio of Platycladus orientalis leaf extract, Polygonum multiflorum extract and oil-controlling factor is 5.8-6.2:0.4-0.6:0.001-0.

1.

2. The composition according to claim 1, characterized in that The composition also includes soybean milk fermentation liquid; The mass ratio of the fermented soybean milk liquid to the Platycladus orientalis leaf extract is 0.001-0.1:5.8-6.

2.

3. The composition according to claim 2, characterized in that The preparation method of the soymilk fermented liquid specifically comprises the following steps: Step 1: Mix soybeans with water, heat, squeeze, sterilize, and filter to obtain soy milk; Step 2: adding the bacterial liquid to soybean milk for fermentation, sterilization, and filtering to obtain a fermentation filtrate, and then mixing the fermentation filtrate with water, butanediol, and 1,2-pentanediol to obtain soybean milk fermentation liquid; In step 1, the mass ratio of soybean to water is 1:8-10; In step 2, the mass ratio of bacterial liquid to soy milk is 1:4-6; The bacterial liquid in step 2 is a fermentation liquid of Lactobacillus bulgaricus, and the fermentation liquid of Lactobacillus bulgaricus is obtained by activating Lactobacillus bulgaricus through an activation medium; In step 2, the mass ratio of the fermentation filtrate to water, butanediol and 1,2-pentanediol is 9-11:76-80:9-11:1-3.

4. Use of the composition according to any one of claims 1 to 3 in preparing cosmetics.

5. A cosmetic, characterized in that: Contains 1 to 10 wt % of the composition according to any one of claims 1 to 3.

6. The cosmetic according to claim 5, characterized in that: The cosmetics also contain water, skin conditioners, surfactants, moisturizers, cooling agents, and preservatives; The mass ratio of water, composition, skin conditioner, surfactant, moisturizer, cooling agent and preservative is 60:6-7:27.5-28.5:0.8-1:3-5:0.4-0.6:0.2-0.

3.

7. The cosmetic according to claim 6, characterized in that: The skin conditioning agent is selected from at least one of ethanol, adenosine, salicylic acid, niacinamide, dextrin, caffeine, cyclodextrin, allantoin, ginseng root fermentation product filtrate, hydrolyzed ginsenosides, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, angelica root extract, liquorice root extract, Centella asiatica extract, Cladosiphon marinum extract, and decapeptide-4; The surfactant is selected from at least one of PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, and PEG-35 castor oil; The moisturizing agent is selected from at least one of glycerin, sodium polyaspartate, 1,2-pentanediol, panthenol, 1,3-propylene glycol, and butylene glycol; The cooling agent is selected from at least one of menthol and menthol lactate; The preservative is selected from at least one of 1,2-hexanediol, phenoxyethanol, ethylhexylglycerin and chlorphenesin.

8. The cosmetic according to claim 5, characterized in that: The cosmetic is emulsion, essence, spray, shampoo or hair mask.

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