Antiallergic probiotic composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-allergic probiotic composition as well as a preparation method and application thereof. The probiotic composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of lactobacillus reuteri PG-LRL116, 10 to 50 parts of lactobacillus acidophilus, 10 to 50 parts of lactobacillus rhamnosus, 10 to 30 parts of lactobacillus gasseri, 10 to 30 parts of lactobacillus salivarius, 5 to 15 parts of cordyceps militaris aqueous extract, 5 to 15 parts of rosemary aqueous extract and 5 to 15 parts of dendrobium huoshanense yeast. The preparation method of the probiotic composition comprises the following steps: preparing the cordyceps militaris aqueous extract, the rosemary aqueous extract and the dendrobium huoshanense yeast; the preparation method comprises the following steps: uniformly mixing lactobacillus reuteri PG-LRL116, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus gasseri, lactobacillus salivarius, a cordyceps militaris aqueous extract, a rosemary aqueous extract and a dendrobium huoshanense fermentation product, so as to prepare the anti-allergic probiotic composition. The probiotic composition disclosed by the invention can be used for effectively inhibiting the occurrence of food sensitization reaction.

Description

Technical Field

[0001] The present invention relates to the technical field of probiotics, and in particular to an anti-allergic probiotic composition, a preparation method and application thereof. Background Art

[0002] Allergy is an immune disease, which is a condition in which the immune function of the human body is imbalanced. At present, allergic diseases caused by food allergens have received widespread attention.

[0003] Allergies caused by food allergens are IgE-mediated type I hypersensitivity reactions. When allergens enter the body, dendritic cells (DCs) will differentiate after being stimulated by TSLP, IL-25, and IL-33 secreted by intestinal epithelial cells. The differentiated cells present the allergens to the initial Th cells, which differentiate into Th2 cells after being stimulated by IL-4. On the one hand, B lymphocytes will be activated, and on the other hand, specific IGE will be produced, putting the body in the sensitization stage.

[0004] Probiotics with anti-allergic function can produce substances such as hydrogen peroxide, organic acids and free fatty acids, which can affect the body's nonspecific immune function, thereby increasing the activity of natural killer (NK) cells, enhancing the secretion of immunoglobulin IgA, and improving the barrier function formed on the skin surface or between cells.

[0005] Therefore, the object of the present invention is to develop a probiotic composition with anti-allergic effect. Summary of the invention

[0006] Based on the technical problems existing in the background technology, the present invention proposes an anti-allergic probiotic composition, a preparation method and an application, which effectively inhibit the occurrence of food allergic reactions.

[0007] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0008] 10-50 parts of Lactobacillus reuteri PG-LRL116, 10-50 parts of Lactobacillus acidophilus, 10-50 parts of Lactobacillus rhamnosus, 10-30 parts of Lactobacillus gasseri, 10-30 parts of Lactobacillus salivarius, 5-15 parts of Cordyceps militaris water extract, 5-15 parts of rosemary water extract, and 5-15 parts of Huoshan Dendrobium officinale fermentation product.

[0009] Preferably, the method for preparing the fermented product of Dendrobium huoshanense comprises the following steps:

[0010] S11: Cut the Huoshan Dendrobium into segments, beat, grind and filter, and sterilize the filtrate for later use;

[0011] S12: inoculating the activated Lactobacillus acidophilus and Lactobacillus salivarius into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0012] Preferably, the mass ratio of Lactobacillus acidophilus to Lactobacillus salivarius is 2:1-4, the added amount of the strain is 0.1-0.5% of the mass of the filtrate; the fermentation temperature is 40-50° C., and the fermentation time is 3-6 hours.

[0013] Preferably, the method for preparing the Cordyceps militaris water extract has the following steps: subjecting the Cordyceps militaris subjected to vacuum low-temperature puffing treatment to microwave water extraction, and concentrating and drying the water extract to obtain the Cordyceps militaris water extract.

[0014] Preferably, the material-liquid ratio is 1:5-15; the microwave power is 300-500W, the time is 30-60min, and the temperature is 40-60°C; the vacuum low-temperature puffing temperature is 40-50°C, the time is 1-2h, and the vacuum pumping times are 5-10 times.

[0015] Preferably, the method for preparing the rosemary water extract comprises the following steps: crushing rosemary leaves and then subjecting the leaves to ultrasonic water extraction, and concentrating and drying the water extract to obtain the rosemary water extract.

[0016] Preferably, the material-liquid ratio is 1:10-20; the ultrasonic power is 100-200 W, the time is 20-40 min, and the temperature is 40-60° C.

[0017] The preparation method of the anti-allergic probiotic composition proposed by the present invention comprises the following steps:

[0018] S21: Preparation of Cordyceps militaris water extract, rosemary water extract and Huoshan Dendrobium officinale fermentation product;

[0019] S22: Evenly mix Lactobacillus reuteri PG-LRL116, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus salivarius, Cordyceps militaris water extract, rosemary water extract and Huoshan dendrobium officinale fermentation product to prepare an anti-allergic probiotic composition.

[0020] The present invention provides an application of the anti-allergic probiotic composition in oral tablets.

[0021] Preferably, the raw materials are included in parts by weight: 100 parts of anti-allergic probiotic composition, 50-100 parts of mannitol cross-linked copolymerized wheat straw co-processed product 500, 40-60 parts of sodium carboxymethyl starch, 1-10 parts of hydroxypropyl methylcellulose phthalate, 1-10 parts of citric acid and 1-10 parts of povidone K30.

[0022] Beneficial technical effects of the present invention:

[0023] (1) The probiotic composition of the present invention comprises Lactobacillus reuteri PG-LRL116, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri and Lactobacillus salivarius, and has good anti-food allergy performance; at the same time, the probiotic composition also comprises Cordyceps militaris water extract, rosemary water extract and Huoshan dendrobium fermentation product, which can further improve the anti-allergic performance of the probiotic composition, and can be applied to anti-allergic preparations such as oral tablets.

[0024] (2) The oral tablets prepared from the probiotic composition of the present invention also include mannitol cross-linked copolymerized wheat starch co-processed product 500 (disintegrant), sodium carboxymethyl starch (disintegrant), hydroxypropyl methylcellulose phthalate (coating material), citric acid (flavoring agent) and povidone K30 (binder). Mannitol cross-linked copolymerized wheat starch co-processed product 500 and sodium carboxymethyl starch as disintegrants can improve the solubility of the oral tablets, thereby facilitating absorption by the human body.

[0025] (3) The Cordyceps militaris water extract of the present invention can induce the expression of iNOS and COX-2 by activating macrophage NF-κB, thereby enhancing the production of NO and the expression of pro-inflammatory cytokines. These cytokines and molecules play a key role in immune response and can enhance the body's immune response; in addition, the Cordyceps militaris water extract can also promote the expression of co-stimulatory molecules, which help to enhance the interaction between T cells and antigen presenting cells, thereby further promoting the immune response. Rosemary water extract is rich in a variety of antioxidants, anti-inflammatory and antibacterial components, such as rosmarinic acid, carnosic acid, ursolic acid, etc.; these components give rosemary extract excellent antioxidant, anti-inflammatory and antibacterial biological activities; in terms of anti-inflammatory, rosemary extract can inhibit the release of inflammatory mediators and the activation of inflammatory cells, thereby reducing inflammatory response; in addition, rosemary extract also has an immunomodulatory effect, which can affect the activation and differentiation of immune cells, thereby regulating the body's immune response. The main component of the fermented product of Dendrobium huoshanense is polysaccharide, and the fermented polysaccharide of Dendrobium huoshanense significantly enhances the scavenging ability of hydroxyl radicals and DPPH radicals, and can inhibit the secretion of cytokines, thereby achieving an anti-allergic effect. The present invention uses the combination of Cordyceps militaris water extract, rosemary water extract and the fermented product of Dendrobium huoshanense to play a synergistic role in improving the anti-allergic effect. The relevant mechanism may be that on the one hand, the three components act together through different signal pathways and molecular mechanisms to affect the activation and differentiation of immune cells, thereby enhancing the body's immune response and allergic reactions; on the other hand, it can also scavenge free radicals, inhibit the release of inflammatory mediators and the differentiation of inflammatory cells, thereby alleviating inflammatory reactions and allergic symptoms. DETAILED DESCRIPTION

[0026] The present invention will be further explained below in conjunction with specific embodiments.

[0027] The Lactobacillus reuteri PG-LRL116 of the present invention was purchased from Shandong Jiacheng Biotechnology Co., Ltd.; Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri and Lactobacillus salivarius were all purchased from Yuanpeptide Biotechnology (Tianjin) Co., Ltd.; Cordyceps militaris, rosemary leaves and Huoshan dendrobium are all commercially available.

[0028] Example 1

[0029] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0030] 30 parts of Lactobacillus reuteri PG-LRL116, 30 parts of Lactobacillus acidophilus, 30 parts of Lactobacillus rhamnosus, 20 parts of Lactobacillus gasseri, 20 parts of Lactobacillus salivarius, 10 parts of Cordyceps militaris water extract, 10 parts of rosemary water extract, and 10 parts of Huoshan Dendrobium fermentation product.

[0031] The steps of preparing the fermented product of Dendrobium huoshanense are as follows:

[0032] S11: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0033] S12: inoculating the activated Lactobacillus acidophilus and Lactobacillus salivarius into the sterilized filtrate of S1 for fermentation. The fermented solution is concentrated and dried to obtain the fermented product of Dendrobium huoshanense. The activation method refers to the instruction manual of the strain.

[0034] The mass ratio of Lactobacillus acidophilus to Lactobacillus salivarius is 1:1, and the added amount of the strain is 0.3% of the mass of the filtrate; the fermentation temperature is 45° C. and the time is 4 hours.

[0035] The method for preparing the water extract of Cordyceps militaris is as follows: extracting the Cordyceps militaris after vacuum low-temperature expansion by microwave, concentrating and drying the water extract to obtain the water extract of Cordyceps militaris. The solid-liquid ratio is 1:10; the microwave power is 400W, the time is 45min, and the temperature is 50°C; the vacuum low-temperature expansion temperature is 45°C, the time is 1.5h, and the vacuum pumping times are 7 times.

[0036] The preparation method of rosemary water extract is as follows: crush rosemary leaves, extract them by ultrasonic water, concentrate and dry the water extract to obtain rosemary water extract, wherein the material-liquid ratio is 1:15, the ultrasonic power is 150W, the time is 30min, and the temperature is 50°C.

[0037] The preparation method of the anti-allergic probiotic composition proposed by the present invention comprises the following steps:

[0038] S21: Preparation of Cordyceps militaris water extract, rosemary water extract and Huoshan Dendrobium officinale fermentation product;

[0039] S22: Evenly mix Lactobacillus reuteri PG-LRL116, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus salivarius, Cordyceps militaris water extract, rosemary water extract and Huoshan dendrobium officinale fermentation product to prepare an anti-allergic probiotic composition.

[0040] Example 2

[0041] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0042] 10 parts of Lactobacillus reuteri PG-LRL116, 10 parts of Lactobacillus acidophilus, 10 parts of Lactobacillus rhamnosus, 10 parts of Lactobacillus gasseri, 10 parts of Lactobacillus salivarius, 5 parts of Cordyceps militaris water extract, 5 parts of rosemary water extract, and 5 parts of Huoshan Dendrobium officinale fermentation product.

[0043] The steps of preparing the fermented product of Dendrobium huoshanense are as follows:

[0044] S11: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0045] S12: inoculating the activated Lactobacillus acidophilus and Lactobacillus salivarius into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0046] The mass ratio of Lactobacillus acidophilus to Lactobacillus salivarius is 2:1, and the added amount of the strain is 0.1% of the mass of the filtrate; the fermentation temperature is 40° C. and the time is 3 hours.

[0047] The method for preparing the water extract of Cordyceps militaris is as follows: extracting the Cordyceps militaris after vacuum low-temperature expansion by microwave, concentrating and drying the water extract to obtain the water extract of Cordyceps militaris. The solid-liquid ratio is 1:5; the microwave power is 300W, the time is 30min, and the temperature is 40°C; the vacuum low-temperature expansion temperature is 40°C, the time is 1h, and the vacuum pumping times are 5 times.

[0048] The preparation method of rosemary water extract is as follows: crush rosemary leaves, extract them by ultrasonic water, concentrate and dry the water extract to obtain rosemary water extract, wherein the solid-liquid ratio is 1:10, the ultrasonic power is 100W, the time is 20min, and the temperature is 40°C.

[0049] The preparation method of the anti-allergic probiotic composition of this embodiment is the same as that of Example 1.

[0050] Example 3

[0051] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0052] 50 parts of Lactobacillus reuteri PG-LRL116, 50 parts of Lactobacillus acidophilus, 50 parts of Lactobacillus rhamnosus, 30 parts of Lactobacillus gasseri, 30 parts of Lactobacillus salivarius, 15 parts of Cordyceps militaris water extract, 15 parts of rosemary water extract, and 15 parts of Huoshan Dendrobium fermentation product.

[0053] The steps of preparing the fermented product of Dendrobium huoshanense are as follows:

[0054] S11: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0055] S12: inoculating the activated Lactobacillus acidophilus and Lactobacillus salivarius into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0056] The mass ratio of Lactobacillus acidophilus to Lactobacillus salivarius is 1:2, and the added amount of the strain is 0.5% of the mass of the filtrate; the fermentation temperature is 50° C. and the time is 6 hours.

[0057] The method for preparing the water extract of Cordyceps militaris is as follows: extracting the Cordyceps militaris after vacuum low-temperature expansion by microwave, concentrating and drying the water extract to obtain the water extract of Cordyceps militaris. The solid-liquid ratio is 1:15; the microwave power is 500W, the time is 60min, and the temperature is 60℃; the vacuum low-temperature expansion temperature is 50℃, the time is 2h, and the vacuum pumping times are 10 times.

[0058] The preparation method of rosemary water extract is as follows: crush rosemary leaves, extract them by ultrasonic water, concentrate and dry the water extract to obtain rosemary water extract, wherein the material-liquid ratio is 1:20, the ultrasonic power is 200W, the time is 40min, and the temperature is 60℃.

[0059] The preparation method of the anti-allergic probiotic composition of this embodiment is the same as that of Example 1.

[0060] Example 4

[0061] The oral tablet containing the anti-allergic probiotic composition provided by the present invention comprises: 100 parts of the anti-allergic probiotic composition, 80 parts of mannitol cross-linked copolymerization wheat and safflower co-processed product 500, 50 parts of sodium carboxymethyl starch, 5 parts of hydroxypropyl methylcellulose phthalate, 5 parts of citric acid and 5 parts of povidone K30.

[0062] Among them, the anti-allergic probiotic composition is prepared according to Example 1.

[0063] Comparative Example 1

[0064] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0065] 30 parts of Lactobacillus reuteri PG-LRL116, 30 parts of Lactobacillus acidophilus, 30 parts of Lactobacillus rhamnosus, 20 parts of Lactobacillus gasseri, 20 parts of Lactobacillus salivarius, 15 parts of Cordyceps militaris water extract, and 15 parts of rosemary water extract.

[0066] The remaining conditions are the same as those in Example 1.

[0067] Comparative Example 2

[0068] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0069] 30 parts of Lactobacillus reuteri PG-LRL116, 30 parts of Lactobacillus acidophilus, 30 parts of Lactobacillus rhamnosus, 20 parts of Lactobacillus gasseri, 20 parts of Lactobacillus salivarius, 15 parts of Cordyceps militaris water extract, and 15 parts of Huoshan Dendrobium officinale fermentation product.

[0070] The remaining conditions are the same as those in Example 1.

[0071] Comparative Example 3

[0072] The anti-allergic probiotic composition proposed by the present invention comprises the following raw materials in parts by weight:

[0073] 30 parts of Lactobacillus reuteri PG-LRL116, 30 parts of Lactobacillus acidophilus, 30 parts of Lactobacillus rhamnosus, 20 parts of Lactobacillus gasseri, 20 parts of Lactobacillus salivarius, 15 parts of rosemary water extract, and 15 parts of Huoshan dendrobium fermentation product.

[0074] The remaining conditions are the same as those in Example 1.

[0075] Comparative Example 4

[0076] The oral tablet containing the anti-allergic probiotic composition provided by the present invention comprises: 100 parts of the anti-allergic probiotic composition, 130 parts of mannitol cross-linked copolymerization maize co-processing material 500, 5 parts of hydroxypropyl methylcellulose phthalate, 5 parts of citric acid and 5 parts of povidone K30.

[0077] Among them, the anti-allergic probiotic composition is prepared according to Example 1.

[0078] Comparative Example 5

[0079] The oral tablet containing the anti-allergic probiotic composition provided by the present invention comprises: 100 parts of the anti-allergic probiotic composition, 130 parts of sodium carboxymethyl starch, 5 parts of hydroxypropyl methylcellulose phthalate, 5 parts of citric acid and 5 parts of povidone K30.

[0080] Among them, the anti-allergic probiotic composition is prepared according to Example 1.

[0081] The anti-allergic properties of the probiotic compositions prepared in Examples 1-3 and Comparative Examples 1-3 were tested.

[0082] The test method for anti-allergic performance is as follows: 80 BALB / c female mice (weight 18-22g) are used as experimental subjects. After 7 days of adaptive feeding, OVA is used as an allergen to establish a food allergy model. The mice are divided into a blank group, a model group and an experimental group (the ratio is the same as that of Example 1-3 and Comparative Example 1-3), with 10 mice in each group.

[0083] On the 1st and 14th days after 7 days of adaptive feeding, the blank group mice were intraperitoneally injected with 200 μL / mouse of a mixture of PBS: aluminum adjuvant = 1:1, and the model group and the experimental group were intraperitoneally injected with 200 μL / mouse of a mixture of OVA (5 mg / kg): PBS: aluminum adjuvant = 2:1:1. From the 27th day, the blank group and the model group mice were gavaged with 200 μL / 20 g of PBS every day. In the experimental group, 20 mg of the probiotic composition prepared in Example 1-3 and Comparative Example 1-3 was dissolved in 200 μL of PBS, and then the experimental group mice were gavaged at 200 μL / 20 g.

[0084] Allergic challenges were performed on the 28th, 32nd, 36th, 40th and 44th days respectively. The mice were fasted for 2 hours before the allergic challenge. The model group and the experimental group were gavaged with 200 μL / 20g of OVA (2.5g / kg, PBS as solvent), and the blank group was gavaged with 200 μL / 20g of PBS. The tail vein serum of the mice was collected within 1 hour after the fifth allergic challenge and refrigerated at -80 degrees Celsius for future use; the eyeball serum of the mice was collected on the 45th day and refrigerated at -80℃ for future use.

[0085] The specific IgE content in the mouse eye serum, and the histamine and mMCP-1 content in the mouse tail vein serum were measured using the Finetest kit. The test results are shown in Table 1.

[0086] Table 1 Anti-allergic performance test results

[0087] Group Specific IgE (ng / mL) Histamine (ng / mL) mMCP-1(pg / mL) Blank Group 37.7 452.4 12.3 Model Group 28328.8 2489.7 942.1 Example 1 7069.3 808.5 464.8 Example 2 7726.1 881.6 476.5 Example 3 7592.4 864.4 471.2 Comparative Example 1 13381.6 1280.9 591.9 Comparative Example 2 12637.2 1318.4 634.7 Comparative Example 3 12302.1 1193.3 607.3

[0088] It can be seen from the test results of Examples 1-3 in Table 1 that the probiotic composition of the present invention can significantly reduce the content of specific IgE, histamine and mMCP-1 in serum, and embodies excellent anti-allergic performance. This is because the probiotic composition of the present invention includes Lactobacillus reuteri PG-LRL116, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri and Lactobacillus salivarius, and has good anti-food allergy performance; at the same time, the probiotic composition also includes Cordyceps militaris water extract, rosemary water extract and Huoshan Dendrobium fermentation product, which can further improve the anti-allergic performance of the probiotic composition. It can be seen from the test results of Example 1 and Comparative Examples 1-3 that the Cordyceps militaris water extract, rosemary water extract and Huoshan Dendrobium fermentation product in the probiotic composition of the present invention have a synergistic promoting effect in improving the anti-allergic effect. The relevant mechanism may be that on the one hand, the three components work together through different signal pathways and molecular mechanisms to affect the activation and differentiation of immune cells, thereby enhancing the body's immune response and reducing allergic reactions; on the other hand, they can also scavenge free radicals, inhibit the release of inflammatory mediators and the differentiation of inflammatory cells, thereby alleviating inflammatory reactions and allergic symptoms.

Claims

1. An anti-allergic probiotic composition, characterized in that: Contains the following raw materials in parts by weight: 10-50 parts of Lactobacillus reuteri PG-LRL116, 10-50 parts of Lactobacillus acidophilus, 10-50 parts of Lactobacillus rhamnosus, 10-30 parts of Lactobacillus gasseri, 10-30 parts of Lactobacillus salivarius, 5-15 parts of Cordyceps militaris water extract, 5-15 parts of rosemary water extract, and 5-15 parts of Huoshan Dendrobium officinale fermentation product.

2. The anti-allergic probiotic composition according to claim 1, characterized in that: The method steps for preparing the Huoshan Dendrobium fermented product are as follows: S11: Cut the Huoshan Dendrobium into segments, beat, grind and filter, and sterilize the filtrate for later use; S12: inoculating the activated Lactobacillus acidophilus and Lactobacillus salivarius into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

3. The anti-allergic probiotic composition according to claim 2, characterized in that: The mass ratio of Lactobacillus acidophilus to Lactobacillus salivarius is 2:1-4, and the added amount of the strains is 0.1-0.5% of the mass of the filtrate; the fermentation temperature is 40-50°C, and the fermentation time is 3-6 hours.

4. The anti-allergic probiotic composition according to claim 1, characterized in that: The method for preparing the Cordyceps militaris water extract comprises the following steps: extracting the Cordyceps militaris after vacuum low-temperature expansion with microwaves, and concentrating and drying the water extract to obtain the Cordyceps militaris water extract.

5. The anti-allergic probiotic composition according to claim 4, characterized in that: The material-liquid ratio is 1:5-15; the microwave power is 300-500W, the time is 30-60min, and the temperature is 40-60°C; the vacuum low-temperature puffing temperature is 40-50°C, the time is 1-2h, and the vacuum pumping times are 5-10 times.

6. The anti-allergic probiotic composition according to claim 1, characterized in that: The method for preparing the rosemary water extract comprises the following steps: crushing rosemary leaves, performing ultrasonic water extraction, and concentrating and drying the water extract to obtain the rosemary water extract.

7. The anti-allergic probiotic composition according to claim 6, characterized in that: The material-liquid ratio is 1:10-20; the ultrasonic power is 100-200W, the time is 20-40min, and the temperature is 40-60℃.

8. The method for preparing the anti-allergic probiotic composition according to any one of claims 1 to 7, characterized in that: The steps are as follows: S21: Preparation of Cordyceps militaris water extract, Rosemary water extract and Huoshan Dendrobium officinale fermentation product; S22: Evenly mix Lactobacillus reuteri PG-LRL116, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus salivarius, Cordyceps militaris water extract, rosemary water extract and Huoshan dendrobium officinale fermentation product to prepare an anti-allergic probiotic composition.

9. Use of the anti-allergic probiotic composition according to any one of claims 1 to 7 in oral tablets.

10. Use of the anti-allergic probiotic composition in oral tablets according to claim 9, characterized in that: The invention comprises the following raw materials in parts by weight: 100 parts of an anti-allergic probiotic composition, 50-100 parts of mannitol cross-linked copolymerized wheat straw co-processed product 500, 40-60 parts of sodium carboxymethyl starch, 1-10 parts of hydroxypropyl methylcellulose phthalate, 1-10 parts of citric acid and 1-10 parts of povidone K30.