8515 results about "Plantlet" patented technology

The invention discloses application of CRISPR/nCas9 mediated site-directed base substitution in a plant. The invention provides a plant genome site-directed edition system. The system comprises a BE3 plant expression carrier (expressing a fusion protein composed of nCas9(D10A), deaminase and a uracil DNA glycosylase inhibitory protein), and rice OsPDS and OsSBEIIb are taken as target genes for verifying the system. Results show that an expected site-directed mutant plant is respectively obtained in the three selected target spots, accurate site mutation of a base is realized in rice, and the highest efficiency reaches about 20%, so that a feasible and effective base substitution method is provided for crop breeding, the method has strong application potential in the aspect of agricultural breeding, and a foundation is provided for rapidly improving important agronomic traits of crops.

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

The invention discloses a short-spike and root-grafting rapid propagation method for camellia azalea. The short-spike and root-grafting rapid propagation method comprises the following steps: selecting general camellia oleifera seeds, sterilizing the general camellia oleifera seeds with potassium permanganate solution and then carrying out stratification, sprouting and anvil culture; after radicles are 3-4 cm in length, grafting the radicles; selecting one-year branches with strongly-grown crown peripheries, full sprout eyes, no pest and disease damage and half lignification to lignification as scions with the length of 2-3 cm; soaking base parts with a plant growth regulator solution; after the scions are grafted, planting the grafted scions in a container bag filled with nutrient-rich substrate on a seed bed in a sunshelter for culturing; and covering, humidifying, shielding and carrying out standard management to form a high-quality grafted container seedling of the camellia azalea. According to the short-spike and root-grafting rapid propagation method, the grafting is realized at the smooth parts of radicles and the root systems of plants are developed, so that the survival rate and the growth amount of nursery stock can be effectively increased and the culture time of the stock is greatly shortened; and the scions are short, and thus the utilization rate and the propagation coefficient of the scions are improved. The short-spike and root-grafting rapid propagation method has the advantages of convenience for operation, high work efficiency and better economic benefit and social benefit; and according to the method, the scale propagation effect of the camellia azalea stock is ensured.

A total nutrient liquid fertilizer containing amino acids comprises, by weights, 6-12 parts of N, 1-6 parts of P, 3-10 parts of K, 1-3 parts of Ca, 1-3 parts of Mg, 9-13 parts of Zn, 2-10 parts of Mn, 1-2 parts of B, 0.5-2 parts of Cu, 0.2-0.5 parts of Mo, 3-10 parts of Fe, 10-15 parts of amino acids, 1-10 parts of a plant growth regulator, 2-5 parts of an organic chelating agent and 30-40 parts of water. The liquid fertilizer provides comprehensive nutrition for plants, enhances the freeze, drought, salt and disease resistance of the plants, enhances the synthesis and translocation of substances in the plants, promotes the formation of adenosine triphosphate (ATP), promotes the photosynthesis and protein synthesis, induces the formation of chlorophyll, promotes the cell protoplasm flow, improves the cell vitality and accelerates the growth of the plants.

The invention relates to a propagating method of Dendrobium officinale Kimura et Migo seedlings, characterized in that, vigorous Dendrobium officinale Kimura et Migo maternal plant is performed with artificial pollination, the obtained fruit is sanitized and cut, seeds are used for bourgeoning culture medium, causing the seeds to bourgeon protocorm and further form young seedling, then the young seedling propagated in s test tube is cultivated by test tube blossom culture medium, the vigorous plant is selected with artificial pollination in the test tube after blossom, cut after fruit ripening with aseptic seeding, causing the seeds to develop into small plant, which is cultivated on good seedling culture medium, mature shoot is formed, the mature shoot is performed with seedling exercising in the natural light, furthermore cultivated into the seedling. The invention is simple in operation, low in cost, greatly shortened in maturing stage of the fruit of Dendrobium officinale Kimura et Migo, high in efficiency and no plant diseases and insect pests, and transplanting success ratio reaches more than 95%.

The present invention provides an efficient and novel maize transformation and regeneration system based on a novel split-seed explant. Mature maize seeds are split longitudinally to form a split-seed explant. The split-seed explant can then be used in transformations to introduce a gene of interest into the maize genome to produce novel maize lines having desired characteristics. The split-seed explant can also be used to generate calli and/or multiple shoots, and rooted plantlets.

The invention discloses a recombinant vector and a non-GMO gene editing plant screening method. An initial carrier in the recombinant vector is a CRISPR/Cas9 carrier which contains sgRNA gene, Cas9 gene and screening label gene and is used for plant gene editing, the recombinant vector carries a BelRNAi expression element, and a hairpin RNA interference fragment interfering with bentazone resistance gene is generated through transcription of the BelRNAi expression element. By introducing the BelRNAi expression element to the initial carrier and conducting bentazone herbicide screening on future generation plants, offspring with target gene mutated are reserved, and it is also ensured that the mutated offspring do not contain transgenic fragments; screening method is cheaper, simpler and more effective.

The invention discloses a rapid propagation method of culturing medical anoectochilus roxburghii tissue through which seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and inoculation, and when a tissue culture sterilized seedling is used as an explant, the sterilized inoculation is directly carried out; thirdly, cultivation; and fourthly, transplantation. The invention uses the middle stem of an anoectochilus roxburghii plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average seedling number of each explant is 4.8; the average net increased number of the leaves of each plant is 3.6; the average increased root number is 2.5 and the average net increased fresh weight is 525.6mg. The growing condition of the tissue culture seedling is quite good after being transplanted to substrate. The survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the tissue culture seedling time of anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing anoectochilus roxburghii seedlings.

The invention provides a Dendrobium offcinale tissue cultured seedling training and domesticating method and aims at using a traditional Chinese medicine material culture and seedling raising technology to conduct three-month effective seedling training and domesticating management to Dendrobium offcinale tissue cultured seedlings in a seedling training greenhouse. Control and management are conducted mainly in six aspects, i.e. the design and the construction of the facilities of the seedling training greenhouse, the selection and the management of seedling training mediums, the management before the tissue cultured seedlings come out of bottles in the seedling training greenhouse, the treatment after the tissue cultured seedlings come out of the bottles in the seedling training greenhouse, the training and the field planting of the tissue cultured seedlings, and the management after the training and the field planting of the tissue cultured seedlings. The survival rate of the tissue cultured seedlings during seedling training is effectively improved to approximately 95 percent from the traditional 70 percent and the survival rate of the Dendrobium offcinale tissue cultured seedlings during seedling training is greatly improved. The seedlings which are trained and domesticated for three months can basically adapt to the growth environment conditions of the greenhouse, the leaves of plants obviously become thicker, the color becomes deeper, the stalks become stronger, the root systems become more robust, the survival rate of the domesticated seedlings after transplanting reaches more than 98 percent and the economic benefit and social benefit are produced very greatly.

The invention discloses a no-pollution standardized high-yield method for cultivating guava, which comprises the steps of field selection, orchard establishment, pruning of guava plants, solid manure management, water management, pest control, and flower and fruit management, wherein in the plant pruning, the plant is pruned into a short-trunk multi-bough round head shape so as to culture a stunting crown; in the solid manure management, basic manure and supplementary fertilizer are combined, wherein the basic manure is applied by combining deep tillage after harvesting in spring, the supplementary organic fertilizer is applied in time after fruit setting, and trace elements are supplemented by spraying fertilizer on the foliage; in the water management, weeds are covered within the range of crown to preserve moisture, and the water-saving irrigation system combining spray irrigation and trickle irrigation is utilized; in the pest control, the comprehensive agricultural measures are utilized to cooperate with spraying of high-efficiency low-toxicity low-residual pesticide, orchard management in winter and spring is actively developed, and physical pesticide control technology is popularized; and in the flower and fruit management, flower thinning and fruit thinning are carried out according to the tree vigor and nutritional status, and the fruits in the whole orchard are filled in bags. The invention has the characteristics of low pesticide residue, high fruit quality and low environmental pollution.

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH355718. The invention thus relates to the plants, seeds and tissue cultures of the variety CH355718, and to methods for producing a corn plant produced by crossing a corn plant of variety CH355718 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH355718.

The invention provides a method for raising seedlings of camellia chrysantha plants by top grafting. By the aid of the method, study for supporting technologies for raising the seedlings of the camellia chrysantha plants by top grafting is carried out in the aspects such as scion, rootstock selection and processing, the grafting time, culture conditions and matters needing attention, and in order to achieve purposes of increasing a survival rate and cultivating excellent strains with both ornamental value and medicinal value, a plurality of key links are improved in an emphasized manner and include that (1), large tea-oil trees at tree ages of 60-80 years are selected as rootstocks; (2), scion cuts are subjected to wax sealing processing in a scion storage period; and (3), as the rootstocks are large and are high in drying degree and crowns of the rootstocks are large, an independent spatial protection measure is adopted after grafting, in other words, protective covers are manufactured according to sizes of the trees. The method has the advantages that problems of low quantity of existing wild camellia chrysantha, extremely low fruit rate, long seedling stage duration, low emergence rate and the like in the prior art are solved, the reproductive cycle of camellia chrysantha can be shortened, seedlings can be yielded from a garden in a period ranging from half a year to a year, the survival rate can reach 95% at least, and the excellent strains with both the ornamental value and the medicinal value can be cultivated within the shortest time.

The invention discloses a method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds, comprising seed bourgeoning, proliferation and differentiation, and sound seedling rootage. A culture medium for the seed bourgeoning includes 1/4 of MS, 15 percent (in volume) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; a culture medium for the proliferation and differentiation comprises 1/2 of MS, 0.5-2.0 mg/L of 6-BA, 0.2 mg/L of NAA, 2 g/L of tryptone, 15 percent (V/V) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; and a culture medium for the sound seedling rootage comprises 1/2 of MS, 1.0 mg/L of NAA, 15 percent (in volume) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4. The method achieves over 45 percent of seed bourgeoning rate and over 90 percent of planting rate and transplantation survival rate.

The invention relates to a transplant-free cutting propagation method. The method is characterized in that a cutting medium and a culture medium are held in different containers, wherein, the container holding the cutting medium is bottomless and the upper caliber of the container is identical with the lower caliber of the container, then the bottomless container holding the cutting medium is placed on a container with a bottom for holding the culture medium, the two containers are separated by a separation device to prevent pathogen in the culture medium from infecting the cutting medium, the separation device can be removed 10-20 days after cutting shoots root, new roots of the cutting shoots in the cutting medium stretch downwards into the culture medium without transplantation, and rooted plants absorb nutrition from the culture medium and continue to grow, thus ensuring 95-100% survival rate of the rooted plants.

The invention discloses a shoot cutting propagation method of robinia pseudoacacia aurea. The shoot cutting propagation method comprises the following steps of: (1) selecting appropriate cutting time; (2) preparing a cutting matrix; (3) preparing a rooting agent; (4) preparing cutting slips; and (5) performing management and (6) transplanting after cuttage. The cutting propagation method provided by the invention is capable of remaining excellent fancy points of the stock plant of robinia pseudoacacia aurea; and the cutting propagation method is high in propagation coefficient, and simple and convenient to operate, and the plants grow strong and tidily; therefore, the shoot cutting propagation method of robinia pseudoacacia aurea has great practice and popularization significance in propagation production of ornamental nursery stocks of robinia pseudoacacia aurea.

The invention relates to a tissue culture and rapid propagation method of acer palmatum. The method comprises the following steps of: after a series of sterilization processes, carrying out isolated culture on acer palmatum stems by adopting NN69 as a basic culture medium; and establishing a rapid propagation system of the acer palmatum by utilizing hormone regulation and control to successfully culture an integrated rooting regeneration plant with a transplant survival rate of about 90 percent, wherein a starting culture medium for inoculating a primary generation comprises the NN69, 0.1mg/L of NAA (Naphthylacetic Acid), 0.3mg/L of TDZ (Thidiazuron) and 30g/L of sucrose with a pH value of 5.8; an enrichment culture medium comprises the NN69, 30g/L of sucrose, 0.1mg/L of NAA and 0.8mg/L of KT-30; and a rooting culture medium comprises 1/2 NN69, 0.2mg/L of NAA, 20g/L of sucrose and 0.2g/L of active carbon. The method overcomes the defects of long conventional seedling culturing period of the acer palmatum, low cuttage propagation coefficient, and the like and realizes the industrial production of acer palmatum nursery stocks.

The invention relates to a potting technology for mangrove forest seedling cultivation, which is characterized by comprising the steps of 1) selecting a seedling cultivation field, wherein muddy inning intertidal zones with weak storm, low salinity and fresh water supply are selected; 2) cultivating the seedlings in a nutrition bag, wherein a seedling cultivation matrix is filled in the nutrition bag, and direct seedling cultivation with the nutrition bag is adopted for vivipary mangrove forest plants; 3) transferring the bagged seedlings into pots, wherein plantlet grows up after the seedling is cultivated for 0.5-1 year in bags according to the method of step 2, and then the plantlet is transplanted to a plastic seedling cultivation pot for seedling cultivation; 4) cultivating the potted seedling, wherein the seedling cultivation is carried out after the plantlet is transplanted into the plastic cultivation pot and arranged in a nursery garden, and water and fertilizer management, reshaping and trimming as well as pest control are strengthened in the seeding cultivation process; and 5) outplanting of the seedling, wherein the mangrove forest seedling is cultivated after the plantlet transplanted in step 4) grow for 1-2 years in the plastic seedling cultivation pot. At the time, the seedlings can be transferred out of the nursery garden to be used for recovery of the mangrove forest of the inshore intertidal zone and greening of the coastal intertidal zone. The seedlings cultivated by the technology disclosed by the invention has the characteristics of being high in degree of lignification of the stem, complete and dense in roots and the like; and compared with plantlets of the mangrove forest, the seedlings have stronger adaptive capacity to hard intertidal zones and severe growing environments.

The invention belongs to the field of potato stock seed culture and particularly relates to a method for culturing potato virus-free test-tube plantlets in a water culture mode. The method comprises the steps of seedling exercising, rooting treatment, nutrition liquid preparation, field planting, culture and transplanting. In aspects of variety, culture density, nutrition liquid concentration and rooting agent concentration, the propagating factor and the strong seedling quantity are increased, corresponding measures are adopted for handling in each link, the propagating factor of virus-free seedlings is improved, healthy and strong virus-free seedlings are obtained, and the soilless culture mode requirements of fog culture, substrates and the like are met, so the healthy and efficient potato stock seed production is reached. The original virus-free, germless and insect-free characteristics of the plantlets are maintained, the characteristics of simple technology, low cost, strong germchits and the like are also realized, in addition, the survival rate of the water culture potato seedlings transplanted to the substrates or other soilless culture (such as fog culture) is very high and can sometimes reach 99 percent, and the production efficiency of potato stock seeds is improved.

The invention relates to a method using the cotyledon node of soybeans as explant to perform soybean organogenesis and plant regeneration, belonging to the technical field of agricultural production. The invention is characterized in that based on the traditional tissue culture, the cotyledon node of soybeans is used as explant to perfect the regeneration system of organogenesis means. The invention has the advantages that based on the selection of effective disinfection technology for soybean seeds, the improvement and the optimization of various medium compositions is outstanding; through the verification of the tissue culture of conventional soybean varieties, the induction, the elongation and the rooting of multiple shoot are effectively promoted; the quality of aseptic seedlings and the differentiation rate of the multiple shoot are improved; the organogenesis and plant regeneration process of the cotyledon node of majority varieties are perfectly realized; solid technical support is provided for the genetic transformation and the molecular improvement of soybeans; and the invention has important practical significance to the effective breeding and health production of the soybeans in China.

The invention discloses a fast tissue culture reproducing method of actinidia eriantha, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) tissue culture medium preparation; (2) explant selection and sterilization; (3) inducing culture; (4) proliferation culture; (5) rooting culture; (6) tissue culture plantlet acclimatization and transplant, and the like. The fast tissue culture reproducing method has high culture medium pertinence and good applicability, the inductivity of an initial sprout of an explant reaches more than 80 percent, the proliferation rate of a successive sprout reaches 2-7 times, the plantlet height can reach 2-5 centimeters for 20-30 days, the quality of tissue culture plantlets is enhanced, the rooting rate reachesmore than 95 percent, and the transplant survival rate reaches more than 95 percent. The invention can be popularized and applied to fruit tree plantlet enterprises.