2564 results about "Plantlet" patented technology

The invention relates to the technical field of gene editing, and particularly provides a method for creating herbicide-resistant rice through gene editing of an OsEPSPS gene promoter region, and the method is characterized in that WT / 29bp single allelic deletion mutation is introduced into the OsEPSPS gene promoter region, i.e., A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; 29bp is deleted from the 112nd site to the 1150th site at the upstream of the initiation codon ATG on one allele, and the other allele is kept unchanged. By introducing the single allelic deletion mutation, the rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants, and the application value is high for guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention provides a planting device for releasing speed-controlled aquatic plants. The planting device comprises a planting pot for planting submerged plants; the main body is of a double-ship structure; the clamping and putting mechanism is arranged on the main body, the clamping and putting mechanism comprises a rack, a conveying mechanism and a grabbing mechanism used for grabbing the planting pots, the grabbing mechanism is arranged on the rack in a sliding mode so as to move left and right, the conveying mechanism conveys the planting pots to the position below the grabbing mechanism, and the grabbing mechanism grabs the planting pots to the planting mechanism; the planting mechanism comprises a hollow sleeve, submerged plants of the conveying mechanism are moved to the position over the hollow sleeve through a grabbing mechanism, an annular pipe and air bags are further embedded in the inner wall of the hollow sleeve, the air bags are evenly and annularly distributed on the inner circumferential wall of the hollow sleeve, and the annular pipe is communicated with the air bags; the air pump is started to convey air into the annular pipe to inflate the air bag, and the air bag expands after being inflated to extrude the planting pot in the hollow sleeve, so that the planting pot slowly descends, and the planting work of plants is completed.

The invention relates to the technical field of plant genetic engineering and genetic breeding, in particular to a method for regulating and controlling thermal morphogenesis of arabidopsis thaliana and application. The method is realized by regulating and controlling an ATG8 gene in arabidopsis thaliana, and the ATG8 gene is a combination of ATG8b and ATG8e, or a combination of ATG8f and ATG8h. The application comprises a thermal morphogenesis response weakening plant obtained through cultivation, or a seed thereof, a propagation material, and a kit for identifying the thermal morphogenesis ability of arabidopsis thaliana and weakening the thermal morphogenesis ability of arabidopsis thaliana. The invention discloses a new function of the ATG8 gene for the first time, and rigorous experiments prove that a plurality of members (ATG8b, ATG8e, ATG8f and ATG8h) of the ATG8 gene family positively regulate thermal morphological establishment of arabidopsis thaliana, so that people's understanding of the effect of autophagy in plant temperature sensing and adaptive growth is expanded. The function of the autophagy core gene ATG8 is combined with the breeding target of crop heat-resistant plant type improvement for the first time, and a brand new technical path is provided.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

A soybean cultivar designated 37050315 is disclosed. The invention relates to the seeds of soybean cultivar 37050315, to the plants of soybean cultivar 37050315, to the plant parts of soybean cultivar 37050315, and to methods for producing progeny of soybean cultivar 37050315. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 37050315. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 37050315, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 37050315 with another soybean cultivar.

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The invention provides a hydrogen-rich water concentration dynamic adjustment method, and relates to the field of concentration adaptive adjustment, and the method comprises the following steps: S1, data acquisition; step S2, data preprocessing; s3, key influence factors are extracted; s4, acquiring the initial concentration of the hydrogen-rich water; and S5, obtaining the optimized concentration of the hydrogen-rich water: obtaining a dynamic concentration adjustment amount, an extreme environment adjustment amount and an implicit stress adjustment amount through reinforcement learning dynamic optimization, extreme environment adaptation and implicit stress early warning, and obtaining the final concentration of the hydrogen-rich water. According to the method, self-adaptive adjustment of the concentration of the hydrogen-rich water can be performed according to the growth state of the plant and environmental factors, the accuracy of the concentration of the applied hydrogen-rich water is ensured, and the problem that normal growth of the plant is affected due to too high or too low concentration of the hydrogen-rich water is avoided.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention discloses an infrared corn drought identification method based on wavelet boundary enhancement, and belongs to the field of agricultural informatization and plant phenotype identification, and the method comprises the following steps: S1, obtaining an infrared image of a corn plant; s2, executing two-dimensional discrete wavelet transform to generate a boundary response diagram; s3, generating a binary mask according to the characteristics of the boundary response diagram to the blade edge; s4, connected domain analysis is executed, candidate leaves are obtained, and independent single-leaf masks are generated; s5, the small holes are removed, a mask is obtained, and the blade curvature is quantified; s6, constructing a multi-dimensional feature vector reflecting the curling characteristics of the blade; and S7, outputting the drought grade corresponding to the corn leaf through the multi-layer perceptron model. By adopting the method, automatic identification and early warning of the early-stage water shortage state of the corn are realized.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention discloses an image processing-based intelligent detection method and system for the degree of a grape air burning disease, and the method comprises the steps: automatically collecting a visible light image of a grape plant, segmenting and recognizing a leaf and fruit region, extracting the color and texture features, and comparing the color and texture features with a pre-stored air burning disease symptom feature library, thereby recognizing an early damaged abnormal region. By calculating the proportion and morphological characteristics of affected areas, a single leaf affected index and a fruit affected grade are generated, the quantitative disease degree grade of the whole plant is obtained through comprehensive evaluation, and a disease development trend obtained through historical image sequence analysis and real-time temperature and humidity data collected by an environment sensor are fused. The early warning of the disease diffusion risk is realized, and the targeted grading farming operation suggestions are generated according to the disease grade, the spatial distribution characteristics and the prediction result. According to the invention, diagnosis and early warning of the grape air cautery are realized, the problems of lagging and strong subjectivity of traditional manual inspection are effectively overcome, and decision support is provided for accurate prevention and control.

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

A novel soybean variety, designated 5PFBT27 is provided. Also provided are the seeds of soybean variety 5PFBT27, cells from soybean variety 5PFBT27, plants of soybean 5PFBT27, and plant parts of soybean variety 5PFBT27. Methods provided include producing a soybean plant by crossing soybean variety 5PFBT27 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and / or a native trait into soybean variety 5PFBT27, methods for producing other soybean varieties or plant parts derived from soybean variety 5PFBT27, and methods of characterizing soybean variety 5PFBT27. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PFBT27 are further provided.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention relates to the technical field of plant protection monitoring, in particular to an alien weed invasion ground-air cooperative confidence grading early warning method and system, and the method comprises the following steps: collecting plant multi-source sensing data of a target region through plant ground-air cooperative sensing equipment; performing preliminary identification on the plant multi-source sensing data to output screening confidence, and screening out a suspected foreign weed region; finely sensing the suspected alien weed area by using the plant ground-air cooperative sensing equipment to obtain multi-dimensional feature data, and determining alien weed plants through classification and recognition; constructing a weed invasion risk diffusion prediction model, and obtaining a normalized invasion risk index of the exotic weed plant; and dividing weed invasion grades according to the normalized invasion risk index, and obtaining early warning confidence of the weed invasion grades to generate foreign weed invasion early warning information. According to the invention, precise monitoring, dynamic early warning and reliable decision making of foreign weed invasion are realized through ground-air cooperation.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

A soybean cultivar designated 38480715 is disclosed. The invention relates to the seeds of soybean cultivar 38480715, to the plants of soybean cultivar 38480715, to the plant parts of soybean cultivar 38480715, and to methods for producing progeny of soybean cultivar 38480715. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 38480715. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 38480715, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 38480715 with another soybean cultivar.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention belongs to the technical field of biology, and particularly relates to an Indel molecular marker related to a corn plant height character and application of the Indel molecular marker. The plant height of a corn plant containing the Indel molecular marker is higher than that of a corn plant without the Indel molecular marker. An effective and reliable molecular marker is provided for identification of the plant height character of corn and prediction of a screening machine, the molecular marker is applied to improved inheritance and breeding of the plant height character of corn, the breeding period can be effectively shortened, the breeding process is accelerated, the breeding efficiency is improved, and the process of screening excellent germplasm resources is accelerated; a new direction is provided for reducing the breeding cost, for example, pollination and breed conservation can be carried out on a corn single plant without the InDel molecular marker, and corn seed production can be carried out. The invention provides a new direction for screening, identifying, breeding and the like of the dwarf corn through a molecular assisted selection means.

A celery cultivar designated TBG 52 is disclosed. The invention relates to the seeds of celery cultivar TBG 52, to the plants of celery cultivar TBG 52, and to methods for producing a celery plant by crossing the cultivar TBG 52 with itself or another celery cultivar. The invention further relates to methods for producing a celery plant containing in its genetic material one or more transgenes and to the transgenic celery plants and plant parts produced by those methods. This invention also relates to celery cultivars or breeding cultivars and plant parts derived from celery cultivar TBG 52, to methods for producing other celery cultivars, lines or plant parts derived from celery cultivar TBG 52, and to the celery plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid celery seeds, plants, and plant parts produced by crossing cultivar TBG 52 with another celery cultivar.

The invention discloses a pennisetum purpureum PpDREB1D-like gene and application thereof in improving cold resistance and biomass of plants, and relates to the technical field of plant genetic engineering. The PpDREB1D-like gene derived from the pennisetum purpureum is cloned and functionally identified for the first time, and experimental verification shows that the PpDREB1D-like gene can increase the cold resistance of plants, the growth state of the plants under cold stress is remarkably improved through overexpression of the PpDREB1D-like gene, the cold resistance of the plants is remarkably reduced through silence of the PpDREB1D-like gene, and the PpDREB1D-like gene can be used for improving the cold resistance of the plants. A molecular foundation is laid for popularization and planting of pasture and energy crops in cold regions, and the method has remarkable agricultural production and ecological value.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The invention discloses a growth-promoting halophilic Clariella sackii BWT 1.2507 strain and a preparation method of the growth-promoting halophilic Clariella sackii BWT 1.2507 strain. The growth-promoting halophilic Clariella sackii BWT 1.2507 strain has the advantages that the growth- The invention relates to a bacillus subtilis BWT1.2507, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on September 28, 2025, the address of the GDMCC is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong province, the postcode is 510070, and the preservation number is GDMCC No: 67048. The halophilic Sacchaffia halmapaa BWT1.2507 is separated and screened from the corn rhizosphere saline-alkali soil, has the functional characteristics of saline-alkali resistance growth, nitrogen fixation, organic phosphorus dissolution, IAA secretion and siderophore production, has obvious application effects in promotion of healthy growth of corn plants and alkali and salt reduction of saline-alkali soil, can be developed as an agricultural microbial agent, and has broad application prospects. The application potential in improvement of saline-alkali soil is huge.

A novel soybean variety, designated 5PKTK65 is provided. Also provided are the seeds of soybean variety 5PKTK65, cells from soybean variety 5PKTK65, plants of soybean 5PKTK65, and plant parts of soybean variety 5PKTK65. Methods provided include producing a soybean plant by crossing soybean variety 5PKTK65 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and / or a native trait into soybean variety 5PKTK65, methods for producing other soybean varieties or plant parts derived from soybean variety 5PKTK65, and methods of characterizing soybean variety 5PKTK65. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PKTK65 are further provided.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention discloses a limonium bicolor gene LbZFP8 and application thereof, and relates to the technical field of biological genetic engineering. The invention relates to a limonium bicolor gene LbZFP8 and application thereof. The limonium bicolor gene LbZFP8 is a gene for coding a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; the biological material is recombinant DNA, an expression cassette, a transposon, a plasmid vector, a virus vector or engineering bacteria. The gene provided by the invention can significantly improve the salt tolerance and oxidation resistance of a target plant by transforming plant cells. Experiments show that under the salt stress condition, the survival rate of the LbZFP8 overexpressed arabidopsis thaliana plant is increased by about 45% compared with that of a control group. Meanwhile, the compound plays a key role in regulating and controlling an active oxygen scavenging system, and the activity of SOD and POD enzymes can be effectively enhanced. The technology provides an important gene resource for cultivating new varieties of crops with strong stress resistance, and has a wide application prospect.

The invention discloses a soybean GmAGO10 gene and an application of the soybean GmAGO10 gene. Wherein the nucleotide sequence of the soybean GmAGO10 gene is as shown in SEQ ID NO: 3. The invention also relates to a protein coded by the soybean GmAGO10 gene, and the amino acid sequence of the protein is shown as SEQ ID NO: 4. The invention further relates to application of the soybean GmAGO10 gene in cultivation of a single-plant high-yield soybean plant, the GmAGO10 mutant soybean plant shows an obvious phenotype of flowering number increase and branch number increase, and the single-plant yield of the GmAGO10 mutant soybean plant is remarkably improved by 79% compared with that of a wild type.